WO2024119809A1

WO2024119809A1 - Phenylketonuria-related gene mutation test kit

Info

Publication number: WO2024119809A1
Application number: PCT/CN2023/105259
Authority: WO
Inventors: 戴俊程; 江玥; 解艳芳; 吴雪琴
Original assignee: 南京亿科人群健康研究院有限公司
Priority date: 2022-12-09
Filing date: 2023-06-30
Publication date: 2024-06-13

Abstract

A phenylketonuria-related gene mutation test kit, comprising a kit body (1) and a kit cover (2), wherein a holding platform (3) is fixedly mounted in the kit body (1), and a plurality of test tube holders are provided at an upper end of the holding platform (3). A pressing bag (9) and an exhaust bag (4) are additionally provided to exhaust air in the sealed kit. By means of applying force to the pressing bag (9) for squeezing, the exhaust bag (4) is inflated and expanded to fill up empty space in the kit and squeeze out the air; and at the same time, a blocking block (7) is used to block an exhaust hole (6) to prevent air from entering from the exhaust hole (6).

Description

A phenylketonuria-related gene mutation detection kit

Technical Field

The present invention relates to the technical field of kits, and more specifically to a kit for detecting phenylketonuria-related gene mutations.

Background technique

Phenylketonuria (PKU) is one of the most common amino acid metabolism disorders and is an autosomal recessive genetic disease. Children with this disease are normal at birth; symptoms usually begin to appear at 3 to 6 months old, and are obvious at 1 year old, with the following manifestations: ① Nervous system: Intellectual development is the most prominent, with an IQ lower than normal, and abnormal behavior, such as excitement, restlessness, depression, etc. There may be minor epileptic seizures, and a few will show increased muscle tone and hyperreflexia of tendons; ② Skin: A few months after birth, due to insufficient melanin synthesis, the hair of the child turns from black to yellow and the skin becomes white; skin eczema is more common; ③ Body odor: Due to the excretion of more phenylacetic acid in urine and sweat, there may be a distinct smell of rat urine.

A test kit is a box used to hold chemical reagents for detecting chemical components, drug residues, virus types, etc. The purpose of the test kit is to enable experimenters to get rid of the tedious reagent preparation and optimization process, so the test kit is generally equipped with corresponding instructions for use. Users can get satisfactory results with no or only a small amount of optimization according to the instructions. Test kits are most widely used in medical and scientific research experiments.

However, at present, there are sealing problems in the use of phenylketonuria-related gene mutation detection kits. Since the reagents stored in the kit need to minimize contact with air, the existing kits cannot effectively expel the air in the kit, and also cannot effectively seal the kit.

Summary of the invention

1. Technical problems to be solved

In view of the problems existing in the prior art, the present invention aims to provide a phenylketonuria-related gene mutation detection kit, which can realize the sealing of the reagent by adding a pressing bag and an exhaust bag. The air in the box is discharged by applying force to the pressing bag to squeeze it, so that the air in the exhaust bag expands, thereby filling the gap in the test kit and squeezing out the air. At the same time, the exhaust hole is blocked by a blocking block to prevent air from entering from the exhaust hole. If air enters from the exhaust hole, it will generate heat when it reacts with oxygen through the oxidation block, and the heat conduction block will conduct the heat to the volatile liquid to vaporize and expand it, thereby further blocking the exhaust hole and preventing air from entering, effectively improving the sealing of the test kit.

2. Technical solution

To solve the above problems, the present invention adopts the following technical solutions.

A phenylketonuria-related gene mutation detection kit comprises a kit body and a kit cover, wherein a placement platform is fixedly installed in the kit body, a plurality of test tube slots are provided at the upper end of the placement platform, test tubes are placed in the test tube slots, a groove is provided at the lower end of the kit cover, an exhaust bag is fixedly connected in the groove, exhaust plates are fixedly connected at both left and right ends of the exhaust bag, exhaust holes are provided in the inner walls of both left and right ends of the groove, a blocking block matching the exhaust hole is threadedly connected at one end of the exhaust plate close to the exhaust hole, a pre-release groove is provided at the upper end of the kit cover, a pressing bag is fixedly connected at the inner end of the pre-release groove, a top plate is provided in the pressing bag, and the top plate passes through the pressing bag, the kit cover and the exhaust bag and extends to In the exhaust bag, the pressing bag is communicated with the exhaust bag, and the upper end of the reagent box cover is slidably connected with a pair of sliding covers, and the pair of sliding covers are respectively located at the left and right ends of the pressing bag, so that the air in the sealed reagent box can be discharged by adding the pressing bag and the exhaust bag, and the air intake in the exhaust bag is expanded by applying force to the pressing bag to fill the gap in the reagent box and squeeze out the air, and at the same time, the exhaust hole is blocked by a blocking block to prevent air from entering from the exhaust hole. If air enters from the exhaust hole, it generates heat by reacting with oxygen through the oxidation block, and the heat is transferred to the volatile liquid by the heat conductive block to make it vaporize and expand, thereby further blocking the exhaust hole to prevent air from entering, effectively improving the sealing of the reagent box.

Further, the blocking block includes a threaded block, the threaded block is threadedly connected to the exhaust plate, an end of the threaded block away from the exhaust plate is fixedly connected to an oxygen consumption block, and an end of the oxygen consumption block away from the threaded block is fixedly connected to an expansion bag, and the blocking block is connected to the exhaust plate through the threaded block, so as to facilitate subsequent replacement. At the same time, the oxygen-consuming block is used to consume the leaked air, and the expansion bag is used to further block the exhaust hole to prevent air from leaking in.

Furthermore, the oxygen-consuming block includes a hollow block, which is fixedly connected to the threaded block. A plurality of through holes are provided at the outer end of the hollow block. A placement cavity is provided in the middle of the hollow block, and the placement cavity is connected to the through holes. An oxidation block is installed in the placement cavity, and air enters the through holes and contacts the oxidation block to consume the air.

Furthermore, the oxidation block is made of a self-heating material, and the oxidation block emits heat while encountering air and consuming oxygen.

Furthermore, the expansion bag includes an expansion membrane, which is fixedly connected to the oxygen-consuming block. The expansion membrane is filled with volatile liquid. The expansion membrane is fixedly connected to a heat-conducting block at one end close to the oxygen-consuming block, and the heat-conducting block is in contact with the oxidation block. Heat is dissipated through the oxidation block and conducted to the volatile liquid through the heat-conducting block, so that the volatile liquid expands due to the heat.

Furthermore, the volatile liquid is made of ammonium bicarbonate solution, and the ammonium bicarbonate solution is vaporized after being heated to expand the expansion membrane, thereby further blocking the exhaust hole.

Furthermore, the top plate includes a short connecting plate, which is arranged in the pressing bag. The lower end of the short connecting plate is fixedly connected to a plurality of vertical plates, and the lower end of the vertical plate is fixedly connected to a long connecting plate, and the long connecting plate is located in the exhaust bag. The short connecting plate is subjected to force against the vertical plate so that the long connecting plate is subjected to force to press down the pressing bag, so that the pressing bag is pre-expanded downward.

Furthermore, the sliding cover includes a cover plate, which is slidably connected to the reagent box cover, and a magnetic block is fixedly connected to one end of the cover plate near the pressing capsule. A limiting groove is provided at the upper end of the reagent box cover, and a limiting block is slidably connected in the limiting groove, and the limiting block is fixedly connected to the cover plate. The limiting block and the cross-sectional end of the limiting groove are both T-shaped. After the pressing capsule is squeezed into the pre-release groove, a pair of sliding covers are moved to slide close to each other, and at the same time, a pair of magnetic blocks are used to adsorb and fix each other.

Furthermore, a sealing gasket is provided at the joint between the reagent box body and the reagent box cover, and the sealing gasket can make the overall sealing of the reagent box better and effectively prevent air from entering.

Furthermore, both left and right ends of the reagent box body are provided with carrying grooves, and anti-skid rubber pads are fixedly connected in the carrying grooves. The carrying grooves are provided to facilitate users to carry the reagent box.

3. Beneficial effects

Compared with the prior art, the advantages of the present invention are:

(1) This solution can achieve the goal of exhausting the air in the sealed test kit by adding a pressing bag and an exhaust bag. By applying force to the pressing bag to squeeze it, the air in the exhaust bag expands, thereby filling the gap in the test kit and squeezing the air out. At the same time, the exhaust hole is blocked by a blocking block to prevent air from entering from the exhaust hole. If air enters from the exhaust hole, it will generate heat when it reacts with oxygen through the oxidation block. The heat is then transferred to the volatile liquid by the heat conductive block to cause it to vaporize and expand, thereby further blocking the exhaust hole and preventing air from entering, thereby effectively improving the sealing of the test kit.

(2) In this solution, the blocking block includes a threaded block, which is threadedly connected to the exhaust plate. An oxygen-consuming block is fixedly connected to one end of the threaded block away from the exhaust plate. An expansion bag is fixedly connected to one end of the oxygen-consuming block away from the threaded block. The blocking block is connected to the exhaust plate through the threaded block to facilitate subsequent replacement. At the same time, the oxygen-consuming block is used to consume the leaked air, and the expansion bag is used to further block the exhaust hole to prevent air from leaking in.

(3) In this scheme, the oxygen-consuming block includes a hollow block, which is fixedly connected to the threaded block. A plurality of through holes are provided at the outer end of the hollow block, and a placement cavity is provided in the middle of the hollow block, which is connected to the through holes. An oxidation block is installed in the placement cavity, and air enters the through holes and contacts the oxidation block, thereby consuming the air.

(4) In this solution, the oxidation block is made of self-heating material, and the oxidation block dissipates heat while encountering air and consuming oxygen.

(5) In this scheme, the expansion bag includes an expansion membrane, which is fixedly connected to the oxygen-consuming block. The expansion membrane is filled with volatile liquid. The expansion membrane is fixedly connected to a heat-conducting block at one end close to the oxygen-consuming block, and the heat-conducting block is in contact with the oxidation block. The heat dissipated by the oxidation block is conducted to the volatile liquid by the heat-conducting block, so that the volatile liquid expands due to the heat.

(6) In this scheme, the volatile liquid is made of ammonium bicarbonate solution, and the ammonium bicarbonate solution is vaporized after being heated to expand the expansion membrane, thereby further blocking the exhaust hole.

(7) In this solution, the top plate includes a short connecting plate, which is arranged in the pressing bag. The lower end of the short connecting plate is fixedly connected to a plurality of vertical plates, and the lower end of the vertical plate is fixedly connected to a long connecting plate, and the long connecting plate is located in the exhaust bag. The short connecting plate is subjected to force to press against the vertical plate so that the long connecting plate is subjected to force to press down the pressing bag, so that the pressing bag is pre-expanded downward.

(8) In the present solution, the sliding cover includes a cover plate, which is slidably connected to the reagent box cover, and a magnetic block is fixedly connected to one end of the cover plate near the pressing capsule. A limiting groove is provided at the upper end of the reagent box cover, and a limiting block is slidably connected in the limiting groove, and the limiting block is fixedly connected to the cover plate. The cross-sectional ends of the limiting block and the limiting groove are both T-shaped. After the pressing capsule is squeezed into the pre-release groove, a pair of sliding covers are moved to slide close to each other, and at the same time, a pair of magnetic blocks are used to adsorb and fix each other.

(9) In this solution, a sealing gasket is provided at the joint between the reagent box body and the reagent box cover. The sealing gasket can make the overall sealing of the reagent box better and effectively prevent air from entering.

(10) In this solution, both left and right ends of the reagent box body are provided with carrying slots, and non-slip rubber pads are fixedly connected to the carrying slots. The opening of the carrying slots makes it convenient for users to carry the reagent box.

BRIEF DESCRIPTION OF THE DRAWINGS

Fig. 1 is a schematic diagram of the overall structure of the present invention;

FIG2 is a front cross-sectional structural schematic diagram of the present invention;

FIG3 is a schematic diagram of the structure of the blocking block of the present invention;

FIG. 4 is a schematic diagram of a side cross-sectional structure of a blocking block according to the present invention.

Description of the numbers in the figure:
1. Test kit body; 2. Test kit cover; 3. Placement platform; 4. Exhaust bag; 5. Exhaust plate; 6.
Exhaust hole; 7. Blocking block; 71. Threaded block; 72. Oxygen-consuming block; 721. Hollow block; 722. Through hole; 723. Oxidation block; 73. Expansion bladder; 731. Expansion membrane; 732. Volatile liquid; 733. Heat-conducting block; 8. Pre-release groove; 9. Pressing bladder; 10. Top plate; 101. Short connecting plate; 102. Vertical plate; 103. Long connecting plate; 11. Sliding cover; 111. Cover plate; 112. Magnetic block; 113. Limiting block; 12. Hand-held slot.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making creative work are within the scope of protection of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "mounted/connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

Embodiment 1:

Please refer to Figures 1-2, a phenylketonuria-related gene mutation detection kit includes a kit body 1 and a kit cover 2, a placement platform 3 is fixedly installed in the kit body 1, a plurality of test tube slots are provided on the upper end of the placement platform 3, test tubes are placed in the test tube slots, a groove is provided at the lower end of the kit cover 2, an exhaust bag 4 is fixedly connected in the groove, exhaust plates 5 are fixedly connected at both ends of the exhaust bag 4, exhaust holes 6 are provided on the inner walls of both ends of the groove, a blocking block 7 matching the exhaust hole 6 is threadedly connected at one end of the exhaust plate 5 close to the exhaust hole 6, a pre-release groove 8 is provided at the upper end of the kit cover 2, a pressing bag 9 is fixedly connected at the inner end of the pre-release groove 8, a top plate 10 is provided in the pressing bag 9, and the top plate 10 passes through the pressing bag 9, the kit cover 2 and the exhaust bag 4 and extends into the exhaust bag 4, the pressing bag 9 is communicated with the exhaust bag 4, and the pressing bag 9 is connected to the exhaust bag 4. The exhaust bag 4 is filled with inert gas, and a pair of sliding covers 11 are slidably connected to the upper end of the reagent box cover 2, and the pair of sliding covers 11 are respectively located at the left and right ends of the pressing bag 9. The air in the sealed reagent box is discharged by adding the pressing bag 9 and the exhaust bag 4, and the air intake in the exhaust bag 4 is expanded by applying force to the pressing bag 9 to fill the gap in the reagent box and squeeze out the air. At the same time, the exhaust hole 6 is blocked by the blocking block 7 to prevent air from entering from the exhaust hole 6.

Please refer to Figure 3. The blocking block 7 includes a threaded block 71, which is threadedly connected to the exhaust plate 5. An oxygen-consuming block 72 is fixedly connected to one end of the threaded block 71 away from the exhaust plate 5, and an expansion bag 73 is fixedly connected to one end of the oxygen-consuming block 72 away from the threaded block 71. The blocking block 7 is connected to the exhaust plate 5 through the threaded block 71 to facilitate subsequent replacement. At the same time, the oxygen-consuming block 72 is used to consume the leaked air, and the expansion bag 73 is used to further block the exhaust hole 6 to prevent air from leaking in.

Please refer to Figure 4. The oxygen-consuming block 72 includes a hollow block 721, which is fixedly connected to the threaded block 71. A plurality of through holes 722 are provided at the outer end of the hollow block 721. A placement cavity is provided in the middle of the hollow block 721, and the placement cavity is communicated with the through hole 722. An oxidation block 723 is installed in the placement cavity. Air enters the through hole 722 and contacts the oxidation block 723 to consume the air. The oxidation block 723 is made of self-heating material. When the oxidation block 723 encounters air and consumes oxygen, it dissipates heat.

The expansion bag 73 includes an expansion membrane 731, which is fixedly connected to the oxygen-consuming block 72. The expansion membrane 731 is filled with a volatile liquid 732. A heat-conducting block 733 is fixedly connected to one end of the expansion membrane 731 close to the oxygen-consuming block 72, and the heat-conducting block 733 abuts against the oxidation block 723. The heat dissipated by the oxidation block 723 is transferred to the volatile liquid 732 by the heat-conducting block 733, so that the volatile liquid 732 expands due to the heat. The volatile liquid 732 is made of ammonium bicarbonate solution, and the expansion membrane 731 expands due to the vaporization of the ammonium bicarbonate solution after being heated, thereby further blocking the exhaust hole 6.

Please continue to refer to Figure 2. The top plate 10 includes a short connecting plate 101, which is arranged in the pressing bag 9. The lower end of the short connecting plate 101 is fixedly connected to a plurality of vertical plates 102. The lower end of the vertical plate 102 is fixedly connected to a long connecting plate 103, and the long connecting plate 103 is located in the exhaust bag 4. The short connecting plate 101 is forced to press against the vertical plate 102 so that the long connecting plate 103 is forced to press down the pressing bag 9, so that the pressing bag 9 is pre-expanded downward.

The sliding cover 11 includes a cover plate 111, which is slidably connected to the reagent box cover 2. The cover plate 111 is fixedly connected to a magnetic block 112 at one end close to the pressing capsule 9. A limiting groove is provided at the upper end of the reagent box cover 2. A limiting block 113 is slidably connected in the limiting groove, and the limiting block 113 is fixedly connected to the cover plate 111. The limiting block 113 and the cross-sectional end of the limiting groove are both T-shaped. After the pressing capsule 9 is squeezed into the pre-release groove 8, a pair of sliding covers 11 are moved to slide close to each other, and at the same time, a pair of magnetic blocks 112 are used to adsorb and fix each other.

Please continue to refer to FIG. 1 . A sealing gasket is provided at the joint between the reagent box body 1 and the reagent box cover 2 . The sealing gasket can make the reagent box as a whole more sealed and effectively prevent air from entering.

Both left and right ends of the reagent box body 1 are provided with carrying grooves 12, and anti-skid rubber pads are fixedly connected in the carrying grooves 12. The carrying grooves 12 are provided to facilitate users to carry the reagent box.

The present invention applies force to the pressing bag 9 to squeeze the air in the exhaust bag 4, so as to fill the gap in the reagent box and squeeze out the air. At the same time, the exhaust hole 6 is blocked by the blocking block 7 to prevent air from entering from the exhaust hole 6. If air enters from the exhaust hole 6, it will generate heat when it meets oxygen through the oxidation block 723. The heat is then conducted to the volatile liquid 732 by the heat conducting block 733 to vaporize and expand it, thereby further blocking the exhaust hole 6 to prevent air from entering, thereby effectively improving the sealing of the reagent box.

The above is only a preferred specific implementation of the present invention; however, the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and its improved conception within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

A phenylketonuria-related gene mutation detection kit comprises a kit body (1) and a kit cover (2), characterized in that: a placement platform (3) is fixedly installed in the kit body (1), a plurality of test tube slots are provided at the upper end of the placement platform (3), test tubes are placed in the test tube slots, a groove is provided at the lower end of the kit cover (2), an exhaust bag (4) is fixedly connected in the groove, exhaust plates (5) are fixedly connected to the left and right ends of the exhaust bag (4), exhaust holes (6) are provided on the inner walls of the left and right ends of the groove, and the exhaust plates (5) are close to the exhaust holes (6). One end is threadedly connected with a blocking block (7) matching the exhaust hole (6); the upper end of the reagent box cover (2) is provided with a pre-release groove (8); the inner end of the pre-release groove (8) is fixedly connected with a pressing bag (9); a top plate (10) is provided in the pressing bag (9); and the top plate (10) penetrates the pressing bag (9), the reagent box cover (2) and the exhaust bag (4) and extends into the exhaust bag (4); the pressing bag (9) is communicated with the exhaust bag (4); the upper end of the reagent box cover (2) is slidably connected with a pair of sliding covers (11), and the pair of sliding covers (11) are respectively located at the left and right ends of the pressing bag (9).
A phenylketonuria-related gene mutation detection kit according to claim 1, characterized in that: the blocking block (7) comprises a threaded block (71), the threaded block (71) is threadedly connected to the exhaust plate (5), the end of the threaded block (71) away from the exhaust plate (5) is fixedly connected to an oxygen consumption block (72), and the end of the oxygen consumption block (72) away from the threaded block (71) is fixedly connected to an expansion bag (73).
A phenylketonuria-related gene mutation detection kit according to claim 2, characterized in that: the oxygen consumption block (72) includes a hollow block (721), the hollow block (721) is fixedly connected to the threaded block (71), a plurality of through holes (722) are provided at the outer end of the hollow block (721), a placement cavity is provided in the middle of the hollow block (721), and the placement cavity is connected to the through hole (722), and an oxidation block (723) is installed in the placement cavity.
The phenylketonuria-related gene mutation detection kit according to claim 3 is characterized in that the oxidation block (723) is made of a self-heating material.
The phenylketonuria-related gene mutation detection kit according to claim 2 is characterized in that: the expansion capsule (73) comprises an expansion membrane (731), and the expansion membrane (731) is connected to the consumable The oxygen block (72) is fixedly connected, the expansion membrane (731) is filled with volatile liquid (732), and the expansion membrane (731) is fixedly connected with a heat conducting block (733) at one end close to the oxygen consuming block (72), and the heat conducting block (733) is in contact with the oxidation block (723).
The phenylketonuria-related gene mutation detection kit according to claim 5, characterized in that the volatile liquid (732) is made of ammonium bicarbonate solution.
A phenylketonuria-related gene mutation detection kit according to claim 1, characterized in that: the top plate (10) includes a short connecting plate (101), the short connecting plate (101) is arranged in the pressing bag (9), the lower end of the short connecting plate (101) is fixedly connected to a plurality of vertical plates (102), the lower end of the vertical plate (102) is fixedly connected to a long connecting plate (103), and the long connecting plate (103) is located in the exhaust bag (4).
A phenylketonuria-related gene mutation detection kit according to claim 1, characterized in that: the sliding cover (11) includes a cover plate (111), the cover plate (111) is slidably connected to the kit cover (2), the cover plate (111) is fixedly connected with a magnetic block (112) at one end close to the pressing capsule (9), a limiting groove is provided at the upper end of the kit cover (2), a limiting block (113) is slidably connected in the limiting groove, and the limiting block (113) is fixedly connected to the cover plate (111), and the limiting block (113) and the cross-sectional end of the limiting groove are both T-shaped.
The phenylketonuria-related gene mutation detection kit according to claim 8 is characterized in that a sealing gasket is provided at the joint between the kit body (1) and the kit cover (2).
A phenylketonuria-related gene mutation detection kit according to claim 1, characterized in that: both left and right ends of the kit body (1) are provided with carrying grooves (12), and anti-slip rubber pads are fixedly connected in the carrying grooves (12).