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• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/20—Bacteria; Culture media therefor
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/20—Bacteria; Culture media therefor
  • C12N1/205—Bacterial isolates
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
  • C12R2001/00—Microorganisms ; Processes using microorganisms
  • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Definitions

• the present disclosure pertains to reduction of greenhouse gas emission.
• the present disclosure provides an environment friendly, non-toxic (green) methanotroph formulation for mitigation of methane emission from lowland rice; and a method of preparing the methanotroph formulation.
• GHGs greenhouse gases
• CO2 carbon dioxide
• N2O nitrous oxide
• Agricultural sector contributes about 20% of the total GHG emissions.
• Rice paddy contributes about 30% of global agricultural CH4 emissions.
• methanogens methane producing archaea
• Methanotrophs methane oxidizing bacteria
• methanogenesis methane producing archaea
• the present invention discloses the use of a novel methanotroph composition for reducing methane emission from lowland ecosystem.
• Objects of the present invention is to reduce methane emission from lowland rice.
• An object of the present invention is to provide an environment friendly, non- toxic formulation for reducing methane emission from lowland rice.
• An object of the present invention is to provide an environment friendly, nontoxic methanotroph formulation for reducing methane emission from lowland rice.
• Another object of the present invention is to provide an environment friendly, non-toxic methanotroph formulation comprising Methylobacterium sp. (MT22_NRRI) for reducing methane emission from lowland rice.
• MT22_NRRI Methylobacterium sp.
• Yet another object of the present invention is to provide a method for reducing methane emission from lowland ecosystem.
• aspects of the present disclosure pertains to reduction of greenhouse gas emission.
• the present disclosure provides an environment friendly, non-toxic (green) methanotroph formulation for mitigation of methane emission from lowland rice; and a method of preparing the methanotroph formulation.
• the present disclosure relates to an environment friendly, non-toxic methanotroph formulation comprising Methylobacterium sp. (MT22_NRRI) for reducing methane emission from lowland rice.
• MT22_NRRI Methylobacterium sp.
• the present disclosure relates to an environment friendly, non-toxic methanotroph formulation for reducing methane emission from lowland rice, said formulation comprises: a) a methanotroph broth in a volume of about 38% - 42% v/w of the formulation; b) tamarind seed coat powder in an amount of 18%-22% wt. of the formulation; c) gum acacia in an amount of 18%-22% wt. of the formulation; d) arrowroot powder in an amount of 4%-5% wt. of the formulation; and e) saw dust in an amount of 45%-55% wt. of the formulation.
• the present disclosure relates to a method for producing an environment friendly, non-toxic methanotroph formulation, said method comprises the steps of: a) providing a methanotroph broth; b) adding and mixing arrowroot to the methanotroph broth; c) adding and mixing gum acacia and tamarind seed coat powder to the arrowroot added methanotroph broth to obtain a pre-final formulation; d) adding saw dust to the pre-final formulation in plurality of parts, followed by slow mixing to obtain powdery form of the methanotroph formulation; and e) air-drying the methanotroph formulation, followed by freeze storing.
• the present disclosure relates to a method for reducing methane emission from rice-based cropping system, said method comprises the step of: applying the methanotroph formulation as claimed in claim 1 at 7 and 45 days after transplanting of rice at a dose of 1.0- 1.5 kg per acre of paddy field.
• FIG. 1 represents the step-wise method of producing the Methanotroph Formulation of the present invention.
• FIG. 2 represents the bar graph of Methane reduction percentage by the application of the Methanotroph Formulation of the present invention as compared to Control recommended dose of fertilizers (RDF) in rice-based cropping system.
• RDF Control recommended dose of fertilizers
• inventive subject matter provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.
• the present disclosure pertains to reduction of greenhouse gas emission.
• the present disclosure provides an environment friendly, non-toxic methanotroph formulation for mitigation of methane emission from lowland ecosystem; and a method of preparing the methanotroph formulation.
• the present invention provides an environment friendly, nontoxic methanotroph formulation reduces methane emission by 1-25% on the overall emission. For example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 18%, 20%, 22%, 25%., Most preferably 4% to 15%.
• the present invention provides an environment friendly, nontoxic methanotroph formulation comprising methanotrophic bacterium for mitigation of methane emission from lowland ecosystem, wherein the bacterium is Methylobacterium sp. (MT22_NRRI) with National Center for Biotechnology Information (NCBI) accession number: MZ683316.
• MT22_NRRI Methylobacterium sp.
• NCBI National Center for Biotechnology Information
• the methanotrophic bacterium is incubated in Nitrate Mineral Salts medium, and the resulting broth after 5 days of incubation having 1 CFU/ml to 15 CFU/ml is used in the methanotroph formulation.
• the methanotrophic broth is used based on the total weight of the formulation in a volume of about at least 29.6%, arrowroot powder 3.7%, tamarind seed coat powder 14.8%, gum acacia 14.8%, and saw dust 37% of the formulation to preparation of methanotroph formulation.
• the methanotroph formulation further comprises tamarind seed coat powder (inhibit methanogenic activities), gum acacia (used as adhesive materials and also as substrate for bacteria), arrowroot powder (as binding agent), and saw dust (used as a filler material).
• 135 g of formulation contains 5 gm of arrowroot powder, 20g of tamarind seed coat powder, 20g of gum acacia, and 50g of saw dust.
• the present invention provides a non-toxic methanotroph formulation for reducing methane emission from lowland rice, said formulation comprises: a) a methanotroph broth in a volume of about 29.6% v/w of the formulation; b) tamarind seed coat powder in an amount of 14.8% wt. of the formulation; c) gum acacia in an amount of 14.8% wt. of the formulation; d) arrow root powder in an amount of 3.7% wt. of the formulation; and e) saw dust in an amount of 37% wt. of the formulation.
• the methanotroph formulation is granular in size ranging about 0.5 mm-2.5 mm.
• the rice-based cropping system is lowland ecosystem.
• the present invention provides a method for producing an environment friendly, non-toxic methanotroph formulation, said method comprises the steps of: a) providing a methanotroph broth; b) adding and mixing arrowroot to the methanotroph broth; c) adding and mixing gum acacia and tamarind seed coat powder to the arrowroot added methanotroph broth to obtain a pre-final formulation; d) adding saw dust to the pre-final formulation in plurality of parts, followed by slow mixing to obtain powdery form of the methanotroph formulation; and e) air-drying the methanotroph formulation, followed by freeze storing.
• the present invention provides a method for reducing methane emission from rice-based cropping system, said method comprises the step of: applying the methanotroph formulation at 7 and 45 days after transplanting of rice at a dose of 1-1.5 kg per acre of paddy field.
• FIG. 1 provides the preparation in a step-wise manner.
• 40 mL of methanotroph broth (after 5 days of incubation in nitrate minimal salts (NMS) media) (10 cfu mL’ ) was taken in a 500 mL of beaker.
• 5 g of arrowroot was added to the broth and mixed properly by using glass rod.
• gum-acacia and tamarind seed coat powder (20 g each) were added and mixed gently.
• 50 g of saw dust (10g saw dust added at a time to the broth and repeated it for 5 times) was added and mixed slowly (restrict mechanical injury), followed by air drying the same overnight in room temperature (25 °C).
• the final product was stored in freeze condition (at 4°C).
• Example 2 Reduction of methane emission by the application of ‘Tamarind- Acacia-based Methanotroph Formulation’ in rice
• ‘Tamarind- Acacia-based Methanotroph Formulation’ was applied in the rice-based cropping system (rice-rice and rice-green gram) in lowland for both the kharif and rabi seasons.
• the percentage of methane emission reduction due to the application of tamarind-acacia-based methanotroph formulation was ranged from 4.6 to 12.1% as compared to the recommended doses of fertilizers (used as control) treatment (FIG. 2).

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Citations (3)

• 2022
  • 2022-11-09 IN IN202211063833A patent/IN202211063833A/en unknown
• 2023
  • 2023-10-17 WO PCT/IB2023/060450 patent/WO2024100477A1/en not_active Ceased

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