WO2024091831A3 - A recombinant spider silk protein and method for tag-free and time-saving purification thereof - Google Patents

A recombinant spider silk protein and method for tag-free and time-saving purification thereof Download PDF

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Publication number
WO2024091831A3
WO2024091831A3 PCT/US2023/077242 US2023077242W WO2024091831A3 WO 2024091831 A3 WO2024091831 A3 WO 2024091831A3 US 2023077242 W US2023077242 W US 2023077242W WO 2024091831 A3 WO2024091831 A3 WO 2024091831A3
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WO
WIPO (PCT)
Prior art keywords
time
spider silk
saving
free
recombinant spider
Prior art date
Application number
PCT/US2023/077242
Other languages
French (fr)
Other versions
WO2024091831A2 (en
Inventor
Hsuan-Chen Wu
Ruei-Ci Wang
Original Assignee
National Taiwan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Taiwan University filed Critical National Taiwan University
Publication of WO2024091831A2 publication Critical patent/WO2024091831A2/en
Publication of WO2024091831A3 publication Critical patent/WO2024091831A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention discloses novel recombinant spider silk proteins that may be purified via tag-free and time-saving methods as well as the method for production and purification thereof. More particularly, the present invention discloses amino acid sequences that may be used to produce recombinant spider silk proteins that provide advantages such as self-healing capabilities, tensile properties comparable with native spider silks, high molecular weight, the ability to form micelle-like structures spontaneously, and the ability to be purified using a cost-saving and time-saving method that does not involve tags or diffusion- and/or affinity-based processes, and produces purified proteins which are not denatured and retain a micelle-like form.
PCT/US2023/077242 2022-10-28 2023-10-19 A recombinant spider silk protein and method for tag-free and time-saving purification thereof WO2024091831A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263420103P 2022-10-28 2022-10-28
US63/420,103 2022-10-28

Publications (2)

Publication Number Publication Date
WO2024091831A2 WO2024091831A2 (en) 2024-05-02
WO2024091831A3 true WO2024091831A3 (en) 2024-06-06

Family

ID=90831833

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/077242 WO2024091831A2 (en) 2022-10-28 2023-10-19 A recombinant spider silk protein and method for tag-free and time-saving purification thereof

Country Status (1)

Country Link
WO (1) WO2024091831A2 (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6620917B1 (en) * 2000-01-20 2003-09-16 The United States Of America As Represented By The Secretary Of The Army Method for the purification and aqueous fiber spinning of spider silks and other structural proteins
US20130023655A1 (en) * 2010-04-08 2013-01-24 Qiagen Gmbh Method for precipitating anionic surfactant ions in the presence of nucleic acids
US8642734B2 (en) * 2009-04-22 2014-02-04 Spiber Technologies Ab Method of producing polymers of spider silk proteins
US9233067B2 (en) * 2009-11-30 2016-01-12 Amsilk Gmbh Silk particles for controlled and sustained delivery of compounds
WO2016172716A2 (en) * 2015-04-24 2016-10-27 The Penn State Research Foundation De novo structural protein design for manufacturing high strength materials
WO2017081239A1 (en) * 2015-11-13 2017-05-18 Spiber Technologies Ab Charge-reversed n-terminal spider silk protein domain and uses thereof
US20200354414A1 (en) * 2018-12-13 2020-11-12 Washington University Split intein mediated protein polymerization for microbial production of materials
WO2021055903A1 (en) * 2019-09-20 2021-03-25 Evolved By Nature, Inc. Edible silk and methods of making and using thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6620917B1 (en) * 2000-01-20 2003-09-16 The United States Of America As Represented By The Secretary Of The Army Method for the purification and aqueous fiber spinning of spider silks and other structural proteins
US8642734B2 (en) * 2009-04-22 2014-02-04 Spiber Technologies Ab Method of producing polymers of spider silk proteins
US9233067B2 (en) * 2009-11-30 2016-01-12 Amsilk Gmbh Silk particles for controlled and sustained delivery of compounds
US20130023655A1 (en) * 2010-04-08 2013-01-24 Qiagen Gmbh Method for precipitating anionic surfactant ions in the presence of nucleic acids
WO2016172716A2 (en) * 2015-04-24 2016-10-27 The Penn State Research Foundation De novo structural protein design for manufacturing high strength materials
WO2017081239A1 (en) * 2015-11-13 2017-05-18 Spiber Technologies Ab Charge-reversed n-terminal spider silk protein domain and uses thereof
US20200354414A1 (en) * 2018-12-13 2020-11-12 Washington University Split intein mediated protein polymerization for microbial production of materials
WO2021055903A1 (en) * 2019-09-20 2021-03-25 Evolved By Nature, Inc. Edible silk and methods of making and using thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PEPE GERALD J, MARCIA G. BURCH, EUGENE D. ALBRECHT: "Localization and Developmental Regulation of 11 -Hydroxysteroid Dehydrogenase-1 and -2 in the Baboon Syncytiotrophoblast*", ENDOCRINOLOGY, vol. 142, no. 1, 1 January 2021 (2021-01-01), pages 68 - 80, XP093181136, DOI: 10.1210/endo.142.1.7877 *
SCHMUCK BENJAMIN; GRECO GABRIELE; BARTH ANDREAS; PUGNO NICOLA M.; JOHANSSON JAN; RISING ANNA: "High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials", MATERIALS TODAY, vol. 50, 8 August 2021 (2021-08-08), AMSTERDAM, NL , pages 16 - 23, XP086893940, ISSN: 1369-7021, DOI: 10.1016/j.mattod.2021.07.020 *

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Publication number Publication date
WO2024091831A2 (en) 2024-05-02

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