WO2024064787A3 - Method for determination of double-stranded rna impurity - Google Patents

Method for determination of double-stranded rna impurity Download PDF

Info

Publication number
WO2024064787A3
WO2024064787A3 PCT/US2023/074734 US2023074734W WO2024064787A3 WO 2024064787 A3 WO2024064787 A3 WO 2024064787A3 US 2023074734 W US2023074734 W US 2023074734W WO 2024064787 A3 WO2024064787 A3 WO 2024064787A3
Authority
WO
WIPO (PCT)
Prior art keywords
stranded rna
double
determination
dsrna
methods
Prior art date
Application number
PCT/US2023/074734
Other languages
French (fr)
Other versions
WO2024064787A2 (en
Inventor
Eddie Wang
Zheng MENG
Original Assignee
Ultragenyx Pharmaceutical Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ultragenyx Pharmaceutical Inc. filed Critical Ultragenyx Pharmaceutical Inc.
Publication of WO2024064787A2 publication Critical patent/WO2024064787A2/en
Publication of WO2024064787A3 publication Critical patent/WO2024064787A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided are ELISA based methods and compositions and kits for use in said methods, for quantitatively detecting the presence of double stranded RNA (dsRNA) in a sample solution, e.g., a sample solution that comprises dsRNA and messenger RNA (mRNA). Also provided are methods of correlating the presence of dsRNA with potential immunogenicity in a host.
PCT/US2023/074734 2022-09-21 2023-09-21 Method for determination of double-stranded rna impurity WO2024064787A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263376544P 2022-09-21 2022-09-21
US63/376,544 2022-09-21

Publications (2)

Publication Number Publication Date
WO2024064787A2 WO2024064787A2 (en) 2024-03-28
WO2024064787A3 true WO2024064787A3 (en) 2024-05-02

Family

ID=90455249

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/074734 WO2024064787A2 (en) 2022-09-21 2023-09-21 Method for determination of double-stranded rna impurity

Country Status (1)

Country Link
WO (1) WO2024064787A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190187031A1 (en) * 2017-12-14 2019-06-20 The Regents Of The University Of Michigan Concentration of analytes
US20190360030A1 (en) * 2002-02-21 2019-11-28 Alere San Diego Inc. Recombinase polymerase amplification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190360030A1 (en) * 2002-02-21 2019-11-28 Alere San Diego Inc. Recombinase polymerase amplification
US20190187031A1 (en) * 2017-12-14 2019-06-20 The Regents Of The University Of Michigan Concentration of analytes

Also Published As

Publication number Publication date
WO2024064787A2 (en) 2024-03-28

Similar Documents

Publication Publication Date Title
EP4293126A3 (en) Analysis of multiple analytes using a single assay
WO2019071051A1 (en) Crispr effector system based diagnostics
KR20180084671A (en) Method of identifying genome-wide off-target sites of base editors by detecting single strand breaks in genomic DNA
WO2018187496A8 (en) Plasma based protein profiling for early stage lung cancer prognosis
EP3988668A3 (en) Kit or device for detecting early stage pancreatic cancer or pancreatic cancer precursor lesions and detection method therefor
CR20220063A (en) Targeted rna editing by leveraging endogenous adar using engineered rnas
WO2022212711A3 (en) Methods for identification and ratio determination of rna species in multivalent rna compositions
PH12014502581B1 (en) Aptamer-based multiplexed assays
WO2018170614A8 (en) New technique for genomic large fragment direct cloning and dna multi-molecular assembly
WO2006017083A3 (en) Methods and compositions for phenotype identification based on nucleic acid methylation
EP3967766A4 (en) Primer probe combination, kit, detection method and application for candida species detection
WO2004111190A3 (en) IN VIVO HIGH THROUGHPUT SELECTION OF EFFECTIVE RNAi PROBES
MX2014006118A (en) Detecting analytes.
AU2013252445A8 (en) Assays, methods and apparatus for assessing RNA disruption
Nilsson et al. High salt buffer improves integrity of RNA after fluorescence-activated cell sorting of intracellular labeled cells
Keller et al. Proteomic and functional analysis of the noncanonical poly (A) polymerase Cid14
MX2020010315A (en) Colocalization-by-linkage sandwich assays.
CA3156351A1 (en) Analyte measurement system
EP3185013A3 (en) Biomarkers for determining an allograft tolerant phenotype
CN103060429A (en) Flight mass spectrum biochip for innate potential assessment and its detection method
Haldrup et al. Large-scale evaluation of SLC18A2 in prostate cancer reveals diagnostic and prognostic biomarker potential at three molecular levels
MX2022000627A (en) Method for determining at least one parameter of a sample composition comprising nucleic acid, such as rna, and optionally particles.
WO2020132316A3 (en) Methods for nucleic acid target enrichment
Mitash et al. MicroRNA-21 could be a molecular marker to predict the recurrence of nonmuscle invasive bladder cancer
WO2024064787A3 (en) Method for determination of double-stranded rna impurity

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23869167

Country of ref document: EP

Kind code of ref document: A2