WO2024050533A3 - Compositions and methods for producing rebaudioside m - Google Patents
Compositions and methods for producing rebaudioside m Download PDFInfo
- Publication number
- WO2024050533A3 WO2024050533A3 PCT/US2023/073344 US2023073344W WO2024050533A3 WO 2024050533 A3 WO2024050533 A3 WO 2024050533A3 US 2023073344 W US2023073344 W US 2023073344W WO 2024050533 A3 WO2024050533 A3 WO 2024050533A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- beta
- compositions
- methods
- reb
- producing rebaudioside
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- GSGVXNMGMKBGQU-PHESRWQRSA-N rebaudioside M Chemical compound C[C@@]12CCC[C@](C)([C@H]1CC[C@@]13CC(=C)[C@@](C1)(CC[C@@H]23)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)C(=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GSGVXNMGMKBGQU-PHESRWQRSA-N 0.000 title 1
- 235000019202 steviosides Nutrition 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 239000004383 Steviol glycoside Substances 0.000 abstract 2
- 229930182488 steviol glycoside Natural products 0.000 abstract 2
- 235000019411 steviol glycoside Nutrition 0.000 abstract 2
- 150000008144 steviol glycosides Chemical class 0.000 abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 abstract 1
- 108010043934 Sucrose synthase Proteins 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 239000000178 monomer Substances 0.000 abstract 1
- 238000005580 one pot reaction Methods 0.000 abstract 1
- 229940013618 stevioside Drugs 0.000 abstract 1
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. The designed beta-1,3-glycosyltransferases can add beta-1,3 linked glucose monomers to steviol glycosides. Specifically, the designed beta-1,3-glycosyltransfersases are used in a one-pot reaction with a beta-1,2-glycosyltransferase and sucrose synthase to convert stevioside and/or Reb A into Reb M.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263374461P | 2022-09-02 | 2022-09-02 | |
| US63/374,461 | 2022-09-02 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2024050533A2 WO2024050533A2 (en) | 2024-03-07 |
| WO2024050533A3 true WO2024050533A3 (en) | 2024-05-10 |
Family
ID=90098800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2023/073344 WO2024050533A2 (en) | 2022-09-02 | 2023-09-01 | Compositions and methods for producing rebaudioside m |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2024050533A2 (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180223264A1 (en) * | 2017-02-03 | 2018-08-09 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| US20200032227A1 (en) * | 2018-07-30 | 2020-01-30 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| WO2022034077A1 (en) * | 2020-08-10 | 2022-02-17 | Inbiose N.V. | Production of alpha-1,3 glycosylated form of fuc-a1,2-gal-r |
| WO2022178145A1 (en) * | 2021-02-17 | 2022-08-25 | Arzeda Corp. | Compositions and methods for producing rebaudioside d |
-
2023
- 2023-09-01 WO PCT/US2023/073344 patent/WO2024050533A2/en unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180223264A1 (en) * | 2017-02-03 | 2018-08-09 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| US20200032227A1 (en) * | 2018-07-30 | 2020-01-30 | Codexis, Inc. | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| WO2022034077A1 (en) * | 2020-08-10 | 2022-02-17 | Inbiose N.V. | Production of alpha-1,3 glycosylated form of fuc-a1,2-gal-r |
| WO2022178145A1 (en) * | 2021-02-17 | 2022-08-25 | Arzeda Corp. | Compositions and methods for producing rebaudioside d |
Non-Patent Citations (2)
| Title |
|---|
| DATABASE Protein 22 May 2013 (2013-05-22), ANONYMOUS: "UDP-glycosyltransferase 76G1 [Stevia rebaudiana]", XP093171529, retrieved from Genbank Database accession no. AGL95113 * |
| DATABASE Protein 23 July 2022 (2022-07-23), ANONYMOUS: "UDP-glycosyltransferase 76G1, partial [Stevia rebaudiana]", XP093171532, retrieved from Genbank Database accession no. UTQ11823 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2024050533A2 (en) | 2024-03-07 |
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