WO2024050465A3 - Compositions and methods for improved grapes with reduced polyphenol oxidase - Google Patents

Compositions and methods for improved grapes with reduced polyphenol oxidase Download PDF

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Publication number
WO2024050465A3
WO2024050465A3 PCT/US2023/073224 US2023073224W WO2024050465A3 WO 2024050465 A3 WO2024050465 A3 WO 2024050465A3 US 2023073224 W US2023073224 W US 2023073224W WO 2024050465 A3 WO2024050465 A3 WO 2024050465A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
grapes
compositions
improved
polyphenol oxidase
Prior art date
Application number
PCT/US2023/073224
Other languages
French (fr)
Other versions
WO2024050465A2 (en
Inventor
Arianne Tremblay
Jeffrey TOUCHMAN
Walter VISS
Shiv Tiwari
Original Assignee
Greenvenus Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenvenus Llc filed Critical Greenvenus Llc
Publication of WO2024050465A2 publication Critical patent/WO2024050465A2/en
Publication of WO2024050465A3 publication Critical patent/WO2024050465A3/en

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/88Vitaceae, e.g. Vitus [grape]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present disclosure is directed to grape cells, plants, plant parts, seeds and fruit comprising a mutation in at least one PPO gene, where said mutations reduce the activity of PPO protein compared to a wild-type. The present disclosure is also directed to beverages and food products made from said plants, plant parts, seeds and fruit, and methods for making the same.
PCT/US2023/073224 2022-09-01 2023-08-31 Compositions and methods for improved grapes with reduced polyphenol oxidase WO2024050465A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263403100P 2022-09-01 2022-09-01
US63/403,100 2022-09-01

Publications (2)

Publication Number Publication Date
WO2024050465A2 WO2024050465A2 (en) 2024-03-07
WO2024050465A3 true WO2024050465A3 (en) 2024-04-25

Family

ID=90098756

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/073224 WO2024050465A2 (en) 2022-09-01 2023-08-31 Compositions and methods for improved grapes with reduced polyphenol oxidase

Country Status (1)

Country Link
WO (1) WO2024050465A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022036074A2 (en) * 2020-08-12 2022-02-17 Greenvenus Llc Rapid generation of plants with desired traits
US20220243217A1 (en) * 2019-06-11 2022-08-04 Pairwise Plants Services, Inc. Methods of producing plants with altered fruit development and plants derived therefrom

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220243217A1 (en) * 2019-06-11 2022-08-04 Pairwise Plants Services, Inc. Methods of producing plants with altered fruit development and plants derived therefrom
WO2022036074A2 (en) * 2020-08-12 2022-02-17 Greenvenus Llc Rapid generation of plants with desired traits

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE NUCLEOTIDE 18 January 1997 (1997-01-18), ANONYMOUS: "Vitis vinifera polyphenol oxidase mRNA, complete cds", XP093167480, Database accession no. U83274 *

Also Published As

Publication number Publication date
WO2024050465A2 (en) 2024-03-07

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