WO2024034866A1 - Composition for preventing hair loss and promoting hair growth including complex fermentation product containing natural vitamins - Google Patents

Abstract

The present invention pertains to a cosmetic composition. A cosmetic composition according to an aspect increases the expression of VEGF and FGF7 in human follicular dermal papilla cells (HFDPCs), and thus can promote the delivery of nutrients to follicular dermal papilla cells and induce the growth of hair root cells and the proliferation of follicular dermal papilla cells. Accordingly, the cosmetic composition is expected to promote hair growth and prevent hair loss.

Description

Composition for preventing hair loss and promoting hair growth of complex fermentation containing natural vitamins

The present invention relates to cosmetic compositions.

In the human body, hair grows from hair follicles on the scalp, and throughout life, the cycle of growth (Anagen), catagen (catagen), and telogen (Telogen) is repeated continuously and goes through creation, growth, and shedding. In the catagen phase of hair follicles, the growth of the hair follicle stops and apoptosis and reorganization of the extracellular matrix occur. As the number of hair follicle cells in the catagen and resting phases increases, hair falls out and does not grow again, resulting in hair loss.

Hair is divided into a visible hair shaft and an invisible hair root. Among the hair roots, the root from which hair grows is called the hair bulb, and the hollow part at the bottom is called the dermal papilla. The dermal papilla is the most important part in the creation and growth of hair. It contains blood vessels and nerves, which promote hair growth by delivering nutrients and growth factors to the hair. In other words, since the death and growth reduction of dermal papilla cells leads to a decrease in the number of hairs and hair loss, proliferation of dermal papilla cells is necessary to maintain and increase normal hair.

Meanwhile, vascular endothelial growth factor (VEGF) is a glycoprotein that promotes angiogenesis by inducing the growth of vascular endothelial cells. VEGF attaches to VEGF receptor-2 (VEGFR-2) present in the dermal papilla and acts as a VEGF- It induces proliferation of dermal papilla cells by activating the 2/ERK pathway. In addition, fibroblast growth factor 7 (FGF 7) is a proliferation factor that regulates the hair production cycle by participating in the growth of the outer part of the hair follicle and the part of the hair that appears on the scalp surface. Therefore, increasing gene expression of VEGF and FGF7 in dermal papilla cells can be of great help in inducing hair growth.

Under this background, the present inventors applied bioconversion technology to soy milk, rye, and soybeans to ferment them and then completed a complex fermentation product by mixing them at a certain ratio, which consists of 7 types of vitamin B group and separated from natural products. Since it contains various active ingredients and has the effect of increasing the expression of VEGF and FGF7 in human dermal papilla cells (HFDPC), it is expected to be used as a cosmetic composition for preventing hair loss and promoting hair growth.

One aspect provides a cosmetic composition for preventing hair loss and/or promoting hair growth, which includes as an active ingredient a complex fermentation product obtained by bioconverting and mixing soymilk, rye, and soybeans.

Another aspect provides a composition for external skin application for preventing hair loss and/or promoting hair growth, comprising the complex fermentation product as an active ingredient.

Another aspect provides a pharmaceutical composition for preventing or treating hair loss, comprising the complex fermentation product as an active ingredient.

Another aspect provides a method for preventing hair loss and promoting hair growth, comprising administering to an individual a composition containing the complex fermentation product as an active ingredient.

One aspect is to provide a cosmetic composition for preventing hair loss and/or promoting hair growth, which includes as an active ingredient a complex fermentation product obtained by bioconverting soymilk, rye, and soybeans and then mixing them.

The term "soymilk" in this specification refers to a colloidal (milk) beverage made by grinding soybeans. It can be made by grinding soybeans soaked in water, pouring water, boiling, and then filtering, or processed commercially available products. You can use soy milk.

The term "rye (Secale cereale)" in this specification refers to an annual crop of the Triticeae family, and more specifically, may refer to the powder of rye fruit (rye powder).

The term “soybean” in this specification refers to the annual herb of the legume family and its fruit, and is widely cultivated as a food crop.

The term "bioconversion" in this specification refers to a method of converting an initial material into a desired material using microorganisms or enzymes. In the present invention, natural products such as soy milk, rye and soybeans are mixed with Lactobacillus . ) may refer to the process of obtaining fermented products through fermentation by strains of the genus or Bacillus .

As used herein, the term “complex fermentation product” may refer to a mixture of fermentation products obtained by fermenting two or more natural products. Here, “fermented product” refers to the result of inoculating natural products such as soy milk, rye, and soybeans with microorganisms and fermenting them under predetermined conditions. The fermented product is a fermented extract extracted with a specific solvent and filtered by physical/chemical methods. Includes all fermentation filtrate, etc. According to one embodiment, the complex fermentation product may refer to a mixture of fermentation product, fermentation extract, or fermentation filtrate obtained by fermenting soy milk, rye, and soybeans with lactic acid bacteria or Bacillus subtilis, respectively.

More specifically, the complex fermentation product may be a useful substance itself produced by extracting the fermentation product, a fermentation product obtained by centrifuging and removing fermentation microorganisms from a culture medium in which the fermentation microorganisms and the fermentation product coexist, and fermentation from the culture medium. A fermented product obtained by filtering microorganisms, a fermented product obtained by sterilizing and filtering fermented microorganisms from the culture medium, a diluted liquid obtained by diluting the fermented product, a material produced by extracting the dried product obtained by drying the fermented product, or any combination thereof. Can include all.

As a strain for producing the fermented product or complex fermented product, strains of the Lactobacillus genus or Bacillus genus may be used. Specifically, the Lactobacillus genus strains include, for example, Lactobacillus casei , Lactobacillus aci. Lactobacillus acidophilus , Lactobacillus plantarum, Lactobacillus fermentum , Lactobacillus curvatus , Lactobacillus confusus , Lactobacillus gasseri ( It may be Lactobacillus gasseri ), Lactobacillus rhamnosus , or any combination thereof, and more specifically, it may be Lactobacillus plantarum, but is not particularly limited thereto.

The Bacillus genus strains include, for example, Bacillus subtilis , Bacillus cereus , Bacillus licheniformis , Bacillus toyoi , and Bacillus coagulans . ), Bacillus polyfermenticus, Bacillus amyloliquefaciens , or any combination thereof, and more specifically, Bacillus subtilis, but is not particularly limited thereto.

According to one embodiment, in the present invention, riboflavin (Vitamin B2), pyridoxine (Vitamin B6), and cyanocobalamin (Vitamin B12) are contained in the lactic acid bacteria/soymilk fermentation filtrate fermented by inoculating soymilk with Lactobacillus plantarum. ), Lactic acid bacteria fermented by inoculating rye powder with Lactobacillus plantarum/thiamine (Vitamin B1), nicotinic acid (Vitamin B3), and pantothenic acid (Vitamin B5) in the fermented rye powder, and Bacillus subtilis fermented on soybeans. It was confirmed that folic acid (Vitamin B9) was contained in the Bacillus subtilis/soybean fermentation extract, and a complex fermentation product was prepared by mixing the fermented products at different ratios.

The complex fermentation product includes cyanocobalamin, pyridoxine, riboflavin, thiamine, nicotinic acid, pantothenic acid, and folic acid. For example, the complex fermentation product may include soymilk fermentation filtrate, rye fermentation, and soybean fermentation extract. . As a more specific example, the complex fermentation product may include 1 to 5 parts by weight of soy milk fermentation filtrate, 1 to 5 parts by weight of rye fermentation product, and 1 to 5 parts by weight of soybean fermentation extract, based on 100 parts by weight of the total complex fermentation product, More specifically, each fermented product may be included in 1:1:1 parts by weight. According to one example, it was confirmed that the effect of increasing VEGF and FGF7 gene expression in human hair papilla cells (HFDPC) was the best when soy milk fermentation filtrate, rye fermentation, and soybean fermentation extract were included in equal parts by weight.

In this specification, the term "natural vitamin" refers to vitamins manufactured through the process of extracting and refining vitamins contained in natural products such as grains, vegetables, and fish, without going through chemical processes, and containing artificial flavors, synthetic colorings, or synthetic preservatives. It is characterized by not doing so. The complex fermented product contains natural vitamins thiamine (Vitamin B1), riboflavin (Vitamin B2), nicotinic acid (Vitamin B3), pantothenic acid (Vitamin B5), pyridoxine (Vitamin B6), and folic acid ( Vitamin B9) and cyanocobalamin (Vitamin B12). In this specification, the term 'natural vitamin' may be used interchangeably with 'fermented vitamin'.

As used herein, the term "Alopecia" refers to the phenomenon of hair falling out from the scalp or the condition in which hair becomes sparse or thin, and the term "Hair Loss Prevention" refers to suppressing or preventing hair loss caused by a decrease in the proportion of hair in the growth phase. Or it means any act of treatment. The hair loss includes Alopecia Areata, Androgenetic Alopecia, Telogen Effluvium, Traumatic Alopecia, Trichotillomania, Pressure Alopecia, Anagen Alopecia, Alopecia Pityriasis, and Alopecia Pityriasis. It may be one or more selected from the group consisting of Alopecia Syphlltiac, Alopecia Seborrhecia, symptomatic alopecia, cicatricial alopecia and congenital alopecia.

The term “hair growth promotion” as used herein refers to any act that promotes the production and growth of hair and ultimately increases the proportion of growing hair in the total hair, and includes all acts of preventing or treating hair loss. The cosmetic composition for preventing hair loss and promoting hair growth is effective in preventing hair loss and promoting hair growth caused by exogenous or endogenous factors. The extrinsic factors include excessive pressure, pulling, hair treatment, ultraviolet rays, etc., and the endogenous factors include genetic factors. Factors include hormonal changes, cell death of dermal papilla cells caused by telomeres, and oxidative stress due to cell function decline.

In addition to the active ingredients disclosed herein, the cosmetic composition may further include functional additives and ingredients included in general cosmetic compositions, and may include commonly used antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, etc. It may further contain auxiliary ingredients and carriers. For example, the cosmetic composition may further include auxiliary ingredients such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, squalane, sodium citrate, and allantoin, and the solvent may include Hexanediol, purified water, etc. may be included. Additionally, the functional additive may include ingredients selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohol, pigments, fragrances, and blood circulation agents. There may be accelerators, cooling agents, restrictors, purified water, etc.

The cosmetic composition according to one aspect includes, for example, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, Hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dressing. It may include formulations of hair lacquer, hair moisturizer, hair mousse, or hair spray, and specifically, shampoo, cleanser, conditioner, treatment, hair pack, rinse, scalp pack, tonic, essence, lotion, cream, oil, It may be a mist or spray formulation, but is not particularly limited thereto.

When the formulation of the cosmetic composition according to one aspect is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or oxidized carrier ingredient. Zinc, etc. may be used.

If the formulation of the cosmetic composition according to one aspect is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as the carrier ingredient, and in the case of a spray, chlorofluoro may be additionally used. May contain propellants such as hydrocarbons, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition according to one aspect is a solution or emulsion, a solvent, solvating agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, and benzyl benzoate. , propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or sorbitan fatty acid ester, etc. can be used.

When the formulation of the composition according to one aspect is a suspension, the carrier component may include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

Another aspect is to provide a composition for external skin application for preventing hair loss and/or promoting hair growth, comprising the complex fermentation product as an active ingredient. The same parts as described above also apply to the external skin composition.

The external skin composition may include anything applied from the outside of the skin, for example, a group consisting of creams, gels, patches, sprays, ointments, warning agents, lotions, linear agents, pasta agents, and cataplasma agents. It may be any one selected from, but is not particularly limited thereto.

In addition to the active ingredients, the external skin preparations include, for example, aqueous ingredients, oil-based ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, skin nutrients or these. Combinations may be included, and these may be appropriately combined by a person skilled in the art as needed. In addition, the skin external preparations include metal sequestrants such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belafamil, licorice extract, glablidin, and calin. hot water extract of fruits, various herbal medicines, medicines such as tocopherol acetate, glythylitic acid, tranexamic acid and its derivatives or salts, vitamin C, magnesium ascorbate phosphate, ascorbate glucoside, arbutin, kojic acid, glucose, frug Sugars such as toss and trehalose may be mixed, but are not particularly limited thereto.

Another aspect is to provide a pharmaceutical composition for preventing or treating hair loss, comprising the complex fermentation product as an active ingredient. The same parts as described above also apply to the pharmaceutical composition.

In addition to the active ingredients disclosed herein, the pharmaceutical composition may further contain pharmaceutical auxiliaries such as preservatives, stabilizers, wetting agents or emulsification accelerators, salts and/or buffers for osmotic pressure adjustment, and other therapeutically useful substances. , can be formulated into various oral or parenteral dosage forms according to conventional methods.

The oral administration agents include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, powders, powders, fine granules, granules, pellets, etc., and these dosage forms contain, in addition to the active ingredient, the surface Activators, diluents (e.g. lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (e.g. silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycol) may include. The tablet may contain a binder such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, and may additionally contain a binder such as starch, agar, alginic acid or its sodium salt. It may contain pharmaceutical additives such as disintegrants, absorbents, colorants, flavoring agents, and sweeteners. Additionally, the tablets can be manufactured by conventional mixing, granulating or coating methods.

The parenteral administration may be in a transdermal dosage form, for example, injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, patches, etc. However, it is not particularly limited thereto.

The pharmaceutical composition may be administered parenterally, rectally, topically, transdermally, subcutaneously, etc., and more specifically, may be administered topically to the scalp.

Determination of the dosage of the pharmaceutical composition is within the skill level of a person skilled in the art, and the daily dosage can be appropriately selected by a person skilled in the art in consideration of various factors such as the degree of progression, onset time, age, health condition, and complications of the subject to be administered. there is.

Another aspect provides a method for preventing hair loss and promoting hair growth, comprising administering to an individual a composition containing as an active ingredient a complex fermentation product obtained by bioconverting soymilk, rye, and soybeans. The same parts as described above also apply to the above method.

Specifically, the composition containing the complex fermentation product of the present invention may be administered to an individual as a cosmetic composition, external skin preparation composition, pharmaceutical composition, or quasi-drug composition. In the administration step, the administration method is not particularly limited and may be appropriately selected depending on the formulation of the composition and those skilled in the art. Specifically, the composition can be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, etc., and more specifically, it can be applied to the scalp or administered topically.

The cosmetic composition according to one aspect increases the expression of VEGF and FGF7 in human follicular dermal papilla cells (HFDPC), promotes nutrient delivery to dermal papilla cells, induces growth of hair root cells, and induces proliferation of dermal papilla cells. This can be expected to promote hair growth and prevent hair loss.

Figure 1 shows the results confirming the effect of increasing VEGF gene expression according to the concentration of the complex fermentation product of the present invention compared to the positive control group AS2P.

Figure 2 shows the results confirming the effect of increasing FGF7 gene expression according to the concentration of the complex fermentation product of the present invention compared to the positive control group AS2P.

Figure 3 shows the results of confirming the effect of increasing VEGF gene expression of the complex fermentation products of Examples 1 to 4 compared to the lactic acid bacteria or Bacillus subtilis culture and natural raw materials (soy milk, rye powder, soybean).

Figure 4 shows the results of confirming the effect of increasing FGF7 gene expression of the complex fermentation products of Examples 1 to 4 compared to lactic acid bacteria or Bacillus subtilis culture and natural raw materials (soy milk, rye powder, soybean).

Figure 5 shows the results confirming the effect of increasing VEGF gene expression of the complex fermented product of Example 1 compared to the vitamin B group (Vitamin B1, B2, B3, B5, B6, B9, B12) and their mixed group.

Figure 6 shows the results confirming the effect of increasing FGF7 gene expression of the complex fermentation of Example 1 compared to the vitamin B group (Vitamin B1, B2, B3, B5, B6, B9, B12) and their mixed group.

Hereinafter, it will be described in more detail through an experimental example. However, these experimental examples are for illustrative purposes only and the scope of the present invention is not limited to these experimental examples.

Experimental Example 1: Preparation of vitamins from fermented extract

Fermented vitamins were manufactured by introducing bio-conversion technology, which combines microbial fermentation with natural products.

Experimental Example 1-1: Lactic acid bacteria/soymilk fermentation filtrate

Soy milk was used by adding 10 times the weight of water to soybeans, pulverizing them in a blender, and filtering them through a 150 mesh filter (104 micron). The fermentation medium was prepared with the following ingredients: 2.0% glucose, 1.0% Na ₂ HPO ₄ , and 0.5% NaH ₂ PO ₄ , and then sterilized by adding 90% soy milk. Lactobacillus plantarum was inoculated into the fermentation medium, and stationary fermentation was performed at 30°C for 24 hours. After completion of fermentation, insoluble substances such as remaining bacterial cells were removed using a continuous centrifuge. The recovered fermented product was neutralized with 2N KOH (pH 6.0), preservative, and filtered for sterilization. Afterwards, it was confirmed that the fermentation filtrate contained riboflavin (Vitamin B2), pyridoxine (Vitamin B6), and cyanocobalamin (Vitamin B12).

Experimental Example 1-2: Lactic acid bacteria/rye powder fermentation product

Rye powder was wet-sterilized after adding 5 times the weight of water. Afterwards, in order to decompose the starch in the rye powder, 0.1% of the weight was added using amylase, and the starch was decomposed at 50-60°C for 24 hours and then used for fermentation. Fermentation medium was prepared with the following ingredients: 2.0% glucose, 1.0% Na ₂ HPO ₄ , and 0.5% NaH ₂ PO ₄ , and then 90% rye powder was added and sterilized. Lactobacillus plantarum was inoculated into the fermentation medium, and stationary fermentation was performed at 30°C for 48 hours. After completion of fermentation, insoluble substances such as remaining bacterial cells were removed using a continuous centrifuge. The recovered fermented product was neutralized with 2N KOH (pH 6.0), preservative, and filtered for sterilization. Afterwards, it was confirmed that the fermented product contained thiamine (Vitamin B1), nicotinic acid (Vitamin B3), and pantothenic acid (Vitamin B5).

Experimental Example 1-3: Bacillus subtilis/soybean fermentation extract

5.0% soybeans were added to purified water and heated at 100°C for 1 hour. Afterwards, it was cooled to remove the solid content and only the extract was recovered. Fermentation medium was prepared with ingredients of 2.0% glucose, 1.0% Na ₂ HPO ₄ and 0.5% NaH ₂ PO ₄ and sterilized by adding 90% soybean extract and 0.5% folic acid. Bacillus subtilis was inoculated into the fermentation medium, and stationary fermentation was performed at 45°C for 72 hours. After completion of fermentation, insoluble substances such as remaining bacterial cells were removed using a continuous centrifuge, and the pH was adjusted to 4 using 2M HCl. Afterwards, 2 times the amount of ethanol was added to recover the solid content of the Bacillus subtilis/soybean fermentation extract. The recovered solid content was neutralized with 2N KOH (pH 6.0), preservative, and filtered for sterilization. Afterwards, it was confirmed that the fermented extract contained high molecular form folic acid (Vitamin B9).

We attempted to confirm the effect of promoting hair growth in complex fermentations in which each fermentation product was mixed at different ratios. Accordingly, the composition ratios of each complex fermentation product (Examples 1 to 4) containing vitamin B group are shown in Table 1 below. It was.

	Lactic acid bacteria/soy milk fermentation product	Lactic acid bacteria/fermented rye powder	Bacillus subtilis/soybean fermentation
Example 1	One	One	One
Example 2	2	One	One
Example 3	One	2	One
Example 4	One	One	2

Experimental Example 2: Confirmation of the effect of increasing the expression of hair growth factors (VEGF and FGF7) of complex fermentation

Experimental Example 2-1: Confirmation of increased expression of VEGF and FGF7 using Real-time PCR (real-time polymerase chain reaction) in HFDPC

To evaluate whether the expression of VEGF and FGF7, factors that induce hair growth in human hair papilla cells (HFDPC), can be induced by processing each complex fermentation product prepared in Experimental Example 1, real-time polymerase chain reaction was used. (Quantitative Real-Time Polymerase Chain Reaction: qRT-PCR) was performed. First, HFDPC was dispensed into a 6-well plate at 3×10 ⁵ cells/well and then cultured for 24 hours under cell culture conditions. After 24 hours of incubation, the medium was discarded and washed with PBS, and the cells were starved using medium containing no medium supplements. The next day, medium containing nothing and the complex fermentation products of Examples 1 to 4 were used. After replacing with the medium containing the culture, additional culture was performed for 24 hours. Afterwards, RNA was isolated from cells using the RNeasy Mini Kit (Qiagen), RNA was quantified at 260 nm using a nanodrop, and 2 ㎍ of RNA was used each to be cycled using an amplifier (C1000 Thermal Cycler, Bio- cDNA was synthesized at Rad, USA). By performing a real-time polymerase chain reaction on a real-time PCR machine using a mixture of synthesized cDNA with target protein VEGF and FGF7 primers and cyanine dye Cybergreen (SYBR Green supermis, Applied Biosystems, USA), the final The expression levels of VEGF and FGF7 genes were evaluated. The sequences of the primers used and reaction conditions are shown in Table 2 below, and the expression level of each gene was finally analyzed through correction for the β-actin gene. AS2P (Ascorbic-acid 2-phosphate, 2.5mM) was used as a positive control.

primer sequence		sequence number	order	reaction conditions
VEGF	F	One	5'-GTGCCCACTGAGGAGTTCAAC-3'	After activating the polymerase at 94°C for 5 minutes, polymerization reaction was performed in 40 cycles at 95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds.
	R	2	5'-CCCTATGTGCTGGCCTTGAT-3'
FGF7		F	3		5'-TCCTGCCAACTTTGCTCTACA-3'
	R	4	5'-CAGGGCTGGAACAGTTCACAT-3'
β-actin	F	5	5'-GGCCATCTCTTGCTCGAAGT-3'
	R	6	5'-GAGACCTTCAACACCCCAGC-3'

Experimental Example 2-2: Confirmation of increase in VEGF and FGF7 expression according to concentration of complex fermentation product

As confirmed in Figures 1 and 2, an increase in VEGF and FGF7 gene expression was confirmed in the group treated with the complex fermentation product of the present invention (Example 1), and in particular, when the complex fermentation product was treated at a concentration of 1%, it was positive. It was confirmed that there was a significant increase in VEGF and FGF7 gene expression compared to the control AS2P.

Experimental Example 2-3: Confirmation of the synergistic effect of increasing VEGF and FGF7 expression according to the content ratio of soymilk fermentation filtrate, rye fermentation, and soybean fermentation extract

The complex fermented products of Examples 1 to 4, which were mixed with the fermented products of Experimental Example 1 at different ratios, had a significantly better effect of increasing VEGF and FGF7 gene expression compared to the lactic acid bacteria or Bacillus subtilis culture and natural raw materials (soy milk, rye powder, soybeans). It was confirmed that, except for Example 2, there was a superior effect of increasing VEGF and FGF7 gene expression compared to AS2P, which was a positive control (FIGS. 3 and 4). In addition, in the case of the complex fermentation product of Example 1, which was shown to be the most effective among them, each vitamin B group (Vitamin B1, B2, B3, B5, B6, B9, B12) and their mixture group (1 each: It was confirmed that there was a significantly better effect of increasing VEGF and FGF7 gene expression compared to (included in 1:1:1:1:1:1 parts by weight) (FIGS. 5 and 6).

The description of the present invention described above is for illustrative purposes, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. will be. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive.

Claims (12)

1. A cosmetic composition for preventing hair loss and promoting hair growth, comprising as an active ingredient a complex fermentation product obtained by bioconversion of soy milk, rye, and soybeans.
2. The cosmetic composition of claim 1, wherein the complex fermentation product includes soymilk fermentation filtrate, rye fermentation product, and soybean fermentation extract.
3. The cosmetic composition of claim 2, wherein the complex fermentation product includes 1 to 5 parts by weight of the soymilk fermentation filtrate, 1 to 5 parts by weight of the rye fermentation product, and 1 to 5 parts by weight of the soybean fermentation extract.
4. The cosmetic composition according to claim 2, wherein the complex fermentation product includes the soymilk fermentation filtrate, rye fermentation product, and soybean fermentation extract in a weight ratio of 1:1:1.
5. The cosmetic composition of claim 2, wherein the soymilk fermentation filtrate is obtained by inoculating the soymilk with Lactobacillus sp. and fermenting it.
6. The cosmetic composition according to claim 2, wherein the fermented rye product is fermented by inoculating rye powder with a Lactobacillus sp. strain.
7. The cosmetic composition of claim 2, wherein the fermented soybean extract is fermented by inoculating the soybean with a Bacillus sp. strain.
8. The method of claim 1, wherein the complex fermentation product contains natural vitamins thiamine (Vitamin B1), riboflavin (Vitamin B2), nicotinic acid (Vitamin B3), pantothenic acid (Vitamin B5), pyridoxine (Vitamin B6), folic acid (Vitamin B9), and cyanoacid. A cosmetic composition containing nocobalamin (Vitamin B12).
9. The cosmetic composition of claim 1, wherein the complex fermentation product increases the expression of VEGF and FGF7 in human follicular dermal papilla cells (HFDPC).
10. A composition for external application for skin for preventing hair loss and promoting hair growth, comprising as an active ingredient a complex fermentation product obtained by bioconverting soymilk, rye, and soybeans.
11. A pharmaceutical composition for preventing or treating hair loss, comprising as an active ingredient a complex fermentation product obtained by bioconverting soymilk, rye, and soybeans.
12. A method for preventing hair loss and promoting hair growth, comprising administering to an individual a composition containing as an active ingredient a complex fermentation product obtained by bioconverting soymilk, rye, and soybeans.

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