WO2024011263A3 - Producing sesquiterpenes and other terpenes using plant-based biomasses - Google Patents

Producing sesquiterpenes and other terpenes using plant-based biomasses Download PDF

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Publication number
WO2024011263A3
WO2024011263A3 PCT/US2023/069879 US2023069879W WO2024011263A3 WO 2024011263 A3 WO2024011263 A3 WO 2024011263A3 US 2023069879 W US2023069879 W US 2023069879W WO 2024011263 A3 WO2024011263 A3 WO 2024011263A3
Authority
WO
WIPO (PCT)
Prior art keywords
plant
terpenes
sesquiterpene
production
nucleotide sequence
Prior art date
Application number
PCT/US2023/069879
Other languages
French (fr)
Other versions
WO2024011263A2 (en
Inventor
Zachary DEMOREST
Quentin Dudley
Jonathan Mayers
Brady KURTZ
Lauren Harrison
Annalisa JORDAN
Rachelle LAPHAM
Original Assignee
Cibus Us Llc
Cibus Europe B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cibus Us Llc, Cibus Europe B.V. filed Critical Cibus Us Llc
Publication of WO2024011263A2 publication Critical patent/WO2024011263A2/en
Publication of WO2024011263A3 publication Critical patent/WO2024011263A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Abstract

Example embodiments are directed to methods for contacting a plant part with a nucleotide sequence comprising a rol gene that induces formation of a plant-based biomass comprising a hairy root structure and a nucleotide sequence encoding an enzyme associated with production of a sesquiterpene, and culturing the plant part to enhance the production of the sesquiterpene and/or another terpene, wherein the production is enhanced as compared to a plant part that has not been contacted with the nucleotide sequence encoding the enzyme. Compositions comprising a sesquiterpene and/or another terpene and methods of using the compositions are also provided.
PCT/US2023/069879 2022-07-08 2023-07-10 Producing sesquiterpenes and other terpenes using plant-based biomasses WO2024011263A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263367991P 2022-07-08 2022-07-08
US63/367,991 2022-07-08

Publications (2)

Publication Number Publication Date
WO2024011263A2 WO2024011263A2 (en) 2024-01-11
WO2024011263A3 true WO2024011263A3 (en) 2024-03-14

Family

ID=89454249

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/069879 WO2024011263A2 (en) 2022-07-08 2023-07-10 Producing sesquiterpenes and other terpenes using plant-based biomasses

Country Status (1)

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WO (1) WO2024011263A2 (en)

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0164888B1 (en) * 1984-05-04 1989-03-15 Japan Synthetic Rubber Co., Ltd. Cell and tissue culture process and apparatus
US20020012976A1 (en) * 1993-11-15 2002-01-31 Yukihito Yukimune Method of producing a taxane-type diterpene and a method of obtaining cultured cells which produce the taxane-type dilterpene at a high rate
US20030106437A1 (en) * 2001-10-19 2003-06-12 Pajunen Esko Juhani Method and apparatus for the continuous biocatalytic conversion of aqueous solutions, having one or more degassing stages
US20150141714A1 (en) * 2012-01-13 2015-05-21 Chromatin, Inc. Engineering plants with rate limiting farnesene metabolic genes
US20160312255A1 (en) * 2013-12-02 2016-10-27 Phyton Holdings, Llc Production of thapsigargins by thapsia cell suspension culture
CN108893482A (en) * 2018-06-22 2018-11-27 中国医学科学院药用植物研究所 Radix Salviae Miltiorrhizae Terpene synthase gene SmTPS8, its cloning primer, expression vector, catalysate and application
US20200123583A1 (en) * 2011-08-08 2020-04-23 Evolva Sa Recombinant Production of Steviol Glycosides
US20200224211A1 (en) * 2005-05-10 2020-07-16 Monsanto Technology Llc Genes and uses for plant improvement
US20210147895A1 (en) * 2014-06-13 2021-05-20 Deinove Method of producing terpenes or terpenoids
US20210180081A1 (en) * 2018-08-24 2021-06-17 Flagship Pioneering Innovations Vi, Llc Methods for manufacturing plant messenger packs
US20210395763A1 (en) * 2018-08-08 2021-12-23 Board Of Trustees Of Michigan State University Improved production of terpenoids using enzymes anchored to lipid droplet surface proteins

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0164888B1 (en) * 1984-05-04 1989-03-15 Japan Synthetic Rubber Co., Ltd. Cell and tissue culture process and apparatus
US20020012976A1 (en) * 1993-11-15 2002-01-31 Yukihito Yukimune Method of producing a taxane-type diterpene and a method of obtaining cultured cells which produce the taxane-type dilterpene at a high rate
US20030106437A1 (en) * 2001-10-19 2003-06-12 Pajunen Esko Juhani Method and apparatus for the continuous biocatalytic conversion of aqueous solutions, having one or more degassing stages
US20200224211A1 (en) * 2005-05-10 2020-07-16 Monsanto Technology Llc Genes and uses for plant improvement
US20200123583A1 (en) * 2011-08-08 2020-04-23 Evolva Sa Recombinant Production of Steviol Glycosides
US20150141714A1 (en) * 2012-01-13 2015-05-21 Chromatin, Inc. Engineering plants with rate limiting farnesene metabolic genes
US20160312255A1 (en) * 2013-12-02 2016-10-27 Phyton Holdings, Llc Production of thapsigargins by thapsia cell suspension culture
US20210147895A1 (en) * 2014-06-13 2021-05-20 Deinove Method of producing terpenes or terpenoids
CN108893482A (en) * 2018-06-22 2018-11-27 中国医学科学院药用植物研究所 Radix Salviae Miltiorrhizae Terpene synthase gene SmTPS8, its cloning primer, expression vector, catalysate and application
US20210395763A1 (en) * 2018-08-08 2021-12-23 Board Of Trustees Of Michigan State University Improved production of terpenoids using enzymes anchored to lipid droplet surface proteins
US20210180081A1 (en) * 2018-08-24 2021-06-17 Flagship Pioneering Innovations Vi, Llc Methods for manufacturing plant messenger packs

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ABOU EL-LEEL OMNIA F, EMAM KHALED A, IBRAHIM HEBA M M: "Genetic study, chemical composition and volatile oil of two parsley cultivars (Petroselinumcrispum L.) Used in botanical gardens and cultivated under Egyptian conditions", NATURE AND SCIENCE, MARSLAND PRESS, EAST LANSING, MI, US, vol. 15, no. 11, 1 January 2017 (2017-01-01), US , pages 83 - 94, XP093150924, ISSN: 1545-0740, DOI: 10.7537/marsnsj151117.11 *

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