WO2023288275A3 - Systems and methods for assessment of nucleobase modifications - Google Patents

Systems and methods for assessment of nucleobase modifications Download PDF

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Publication number
WO2023288275A3
WO2023288275A3 PCT/US2022/073737 US2022073737W WO2023288275A3 WO 2023288275 A3 WO2023288275 A3 WO 2023288275A3 US 2022073737 W US2022073737 W US 2022073737W WO 2023288275 A3 WO2023288275 A3 WO 2023288275A3
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WO
WIPO (PCT)
Prior art keywords
interest
fret
protein
methods
acceptor
Prior art date
Application number
PCT/US2022/073737
Other languages
French (fr)
Other versions
WO2023288275A9 (en
WO2023288275A2 (en
Inventor
Alex CHIALASTRI
Siddharth S. DEY
Original Assignee
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Publication of WO2023288275A2 publication Critical patent/WO2023288275A2/en
Publication of WO2023288275A3 publication Critical patent/WO2023288275A3/en
Publication of WO2023288275A9 publication Critical patent/WO2023288275A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present application provides compounds and methods for identifying a modulator of a protein of interest using TR-FRET donor attached to the protein of interest and a tracer containing TR-FRET acceptor. Disclosed assay platforms utilize TR-FRET donors as well as small-molecule ligands labeled with a FRET-acceptor that are both capable of binding to a protein of interest. These platforms address unmet needs and greatly enable drug development efforts for various pharmacologically relevant targets. Small molecule modulators of proteins of interest that were discovered on the basis of the novel assay platforms are also disclosed.
PCT/US2022/073737 2021-07-14 2022-07-14 Systems and methods for assessment of nucleobase modifications WO2023288275A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163221643P 2021-07-14 2021-07-14
US63/221,643 2021-07-14

Publications (3)

Publication Number Publication Date
WO2023288275A2 WO2023288275A2 (en) 2023-01-19
WO2023288275A3 true WO2023288275A3 (en) 2023-03-23
WO2023288275A9 WO2023288275A9 (en) 2024-02-29

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Family Applications (1)

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PCT/US2022/073737 WO2023288275A2 (en) 2021-07-14 2022-07-14 Systems and methods for assessment of nucleobase modifications

Country Status (1)

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WO (1) WO2023288275A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150322107A1 (en) * 2011-05-18 2015-11-12 Cayman Chemical Company, Incorporated Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins
WO2016201374A1 (en) * 2015-06-11 2016-12-15 University Of Florida Research Foundation, Incorporated Sulfanide adenosine derivatives and uses thereof
US20170283453A1 (en) * 2014-08-29 2017-10-05 Katholieke Universiteit Leuven Cofactor analogues for methyltransferases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150322107A1 (en) * 2011-05-18 2015-11-12 Cayman Chemical Company, Incorporated Fluorescent molecular probes for use in assays that measure test compound competitive binding with sam-utilizing proteins
US20170283453A1 (en) * 2014-08-29 2017-10-05 Katholieke Universiteit Leuven Cofactor analogues for methyltransferases
WO2016201374A1 (en) * 2015-06-11 2016-12-15 University Of Florida Research Foundation, Incorporated Sulfanide adenosine derivatives and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FEDERICO STEPHANIE, LASSIANI LUCIA, SPALLUTO GIAMPIERO: "Chemical Probes for the Adenosine Receptors", PHARMACEUTICALS, M D P I AG, CH, vol. 12, no. 4, 1 December 2019 (2019-12-01), CH , pages 168 - 21, XP093049922, ISSN: 1424-8247, DOI: 10.3390/ph12040168 *
URI ASKO, RAIDARU GERDA, SUBBI JUHAN, PADARI KÄRT, POOGA MARGUS: "Identification of the ability of highly charged nanomolar inhibitors of protein kinases to cross plasma membranes and carry a protein into cells", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, ELSEVIER, AMSTERDAM NL, vol. 12, no. 16, 1 August 2002 (2002-08-01), Amsterdam NL , pages 2117 - 2120, XP093049925, ISSN: 0960-894X, DOI: 10.1016/S0960-894X(02)00337-2 *
YANG XUE; HEITMAN LAURA H.; IJZERMAN ADRIAAN P.; VAN DER ES DAAN: "Molecular probes for the human adenosine receptors", PURINERGIC SIGNALLING, SPRINGER VERLAG,, DE, vol. 17, no. 1, 12 December 2020 (2020-12-12), DE , pages 85 - 108, XP037449507, ISSN: 1573-9538, DOI: 10.1007/s11302-020-09753-8 *

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Publication number Publication date
WO2023288275A9 (en) 2024-02-29
WO2023288275A2 (en) 2023-01-19

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