WO2023287363A3 - High sensitivity lateral flow immunoassay for detection of analyte in samples - Google Patents

High sensitivity lateral flow immunoassay for detection of analyte in samples Download PDF

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Publication number
WO2023287363A3
WO2023287363A3 PCT/SG2022/050500 SG2022050500W WO2023287363A3 WO 2023287363 A3 WO2023287363 A3 WO 2023287363A3 SG 2022050500 W SG2022050500 W SG 2022050500W WO 2023287363 A3 WO2023287363 A3 WO 2023287363A3
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WO
WIPO (PCT)
Prior art keywords
analyte
binding molecule
analyte binding
samples
detection
Prior art date
Application number
PCT/SG2022/050500
Other languages
French (fr)
Other versions
WO2023287363A2 (en
Inventor
Jian Hang Danny TNG
Khee Chee Soo
Eng Eong Ooi
Lee Kiang Melvin CHUA
Yong Zhang
Guek-Hong Jenny LOW
Original Assignee
National University Of Singapore
Singapore Health Services Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Of Singapore, Singapore Health Services Pte Ltd filed Critical National University Of Singapore
Publication of WO2023287363A2 publication Critical patent/WO2023287363A2/en
Publication of WO2023287363A3 publication Critical patent/WO2023287363A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method and device for detecting an analyte in a liquid sample, including applying the liquid sample on a permeable material defining at least a first channel in a first portion, and a second portion, said first portion being the site for application of the liquid sample, and for a set of conjugates movably supported therein, wherein said set of conjugates each comprises a first analyte binding molecule coupled to a detectable particle, said second portion being the site for detecting the presence of the detectable particle and comprising a second analyte binding molecule immobilized therein which specifically binds to the analyte, wherein the first analyte binding molecule or the second analyte binding molecule comprises two or more different analyte binding molecules directed to the same analyte and/or analyte binding molecule. The first portion may comprise two parallel flow channels, wherein the second channel contains amplification nanoparticles.
PCT/SG2022/050500 2021-07-16 2022-07-15 High sensitivity lateral flow immunoassay for detection of analyte in samples WO2023287363A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10202107837T 2021-07-16
SG10202107837T 2021-07-16

Publications (2)

Publication Number Publication Date
WO2023287363A2 WO2023287363A2 (en) 2023-01-19
WO2023287363A3 true WO2023287363A3 (en) 2023-03-23

Family

ID=84922597

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG2022/050500 WO2023287363A2 (en) 2021-07-16 2022-07-15 High sensitivity lateral flow immunoassay for detection of analyte in samples

Country Status (1)

Country Link
WO (1) WO2023287363A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019187271A1 (en) * 2018-03-30 2019-10-03 株式会社バイオメディカル研究所 Testing kit and testing method
CN111303254A (en) * 2020-02-20 2020-06-19 北京新创生物工程有限公司 Novel coronavirus (SARS-CoV-2) antigen detection kit
CN112415201A (en) * 2020-08-21 2021-02-26 北京现代高达生物技术有限责任公司 Novel coronavirus S protein and N protein joint detection colloidal gold test strip and preparation method and application thereof
US20210072239A1 (en) * 2018-04-23 2021-03-11 Zeus Co., Ltd. Biosensor comprising linker material and quantum dot beads, and target antigen detection method using same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019187271A1 (en) * 2018-03-30 2019-10-03 株式会社バイオメディカル研究所 Testing kit and testing method
US20210072239A1 (en) * 2018-04-23 2021-03-11 Zeus Co., Ltd. Biosensor comprising linker material and quantum dot beads, and target antigen detection method using same
CN111303254A (en) * 2020-02-20 2020-06-19 北京新创生物工程有限公司 Novel coronavirus (SARS-CoV-2) antigen detection kit
CN112415201A (en) * 2020-08-21 2021-02-26 北京现代高达生物技术有限责任公司 Novel coronavirus S protein and N protein joint detection colloidal gold test strip and preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TNG, D.J.H. ET AL.: "Amplified parallel antigen rapid test for point-of-care salivary detection of SARS-CoV-2 with improved sensitivity", MICROCHIMICA ACTA, vol. 189, no. 1, 6 December 2021 (2021-12-06), pages 14, XP037636199, [retrieved on 20230210], DOI: 10.1007/S00604-021-05113-4 *

Also Published As

Publication number Publication date
WO2023287363A2 (en) 2023-01-19

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DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
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