WO2023282732A1 - Therapeutic and prophylactic toothpaste composition - Google Patents
Therapeutic and prophylactic toothpaste composition Download PDFInfo
- Publication number
- WO2023282732A1 WO2023282732A1 PCT/KZ2021/000020 KZ2021000020W WO2023282732A1 WO 2023282732 A1 WO2023282732 A1 WO 2023282732A1 KZ 2021000020 W KZ2021000020 W KZ 2021000020W WO 2023282732 A1 WO2023282732 A1 WO 2023282732A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- extract
- therapeutic
- toothpaste
- prophylactic
- sodium bicarbonate
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 11
- 239000000606 toothpaste Substances 0.000 title abstract description 14
- 229940034610 toothpaste Drugs 0.000 title abstract description 14
- 230000001225 therapeutic effect Effects 0.000 title abstract description 6
- 230000000069 prophylactic effect Effects 0.000 title abstract description 5
- 239000000284 extract Substances 0.000 claims abstract description 30
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 16
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 12
- 235000002020 sage Nutrition 0.000 claims abstract description 11
- 235000014134 echinacea Nutrition 0.000 claims abstract description 10
- 244000042664 Matricaria chamomilla Species 0.000 claims abstract description 9
- 235000007232 Matricaria chamomilla Nutrition 0.000 claims abstract description 9
- 235000011187 glycerol Nutrition 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229940073507 cocamidopropyl betaine Drugs 0.000 claims abstract description 7
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910052622 kaolinite Inorganic materials 0.000 claims abstract description 7
- 239000000341 volatile oil Substances 0.000 claims abstract description 7
- 239000000230 xanthan gum Substances 0.000 claims abstract description 7
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 7
- 229940082509 xanthan gum Drugs 0.000 claims abstract description 7
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 7
- 239000008213 purified water Substances 0.000 claims abstract description 6
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 6
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 6
- 240000004530 Echinacea purpurea Species 0.000 claims description 9
- 235000007866 Chamaemelum nobile Nutrition 0.000 claims description 7
- 241000721662 Juniperus Species 0.000 claims description 7
- 235000013399 edible fruits Nutrition 0.000 claims description 7
- 210000000214 mouth Anatomy 0.000 claims description 7
- 244000246386 Mentha pulegium Species 0.000 claims description 5
- 235000016257 Mentha pulegium Nutrition 0.000 claims description 5
- 235000004357 Mentha x piperita Nutrition 0.000 claims description 5
- 235000001050 hortel pimenta Nutrition 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 230000002265 prevention Effects 0.000 claims description 3
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 6
- 235000006679 Mentha X verticillata Nutrition 0.000 abstract description 3
- 235000002899 Mentha suaveolens Nutrition 0.000 abstract description 3
- 235000001636 Mentha x rotundifolia Nutrition 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 240000007164 Salvia officinalis Species 0.000 abstract description 2
- 235000002912 Salvia officinalis Nutrition 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000002087 whitening effect Effects 0.000 abstract description 2
- 241000911175 Citharexylum caudatum Species 0.000 abstract 1
- 244000133098 Echinacea angustifolia Species 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000000177 juniperus communis l. berry Substances 0.000 abstract 1
- 230000000144 pharmacologic effect Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 210000002919 epithelial cell Anatomy 0.000 description 6
- 239000012141 concentrate Substances 0.000 description 5
- 210000005087 mononuclear cell Anatomy 0.000 description 5
- 210000000440 neutrophil Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000000202 analgesic effect Effects 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 210000002200 mouth mucosa Anatomy 0.000 description 4
- 239000006072 paste Substances 0.000 description 4
- 230000007170 pathology Effects 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000000845 anti-microbial effect Effects 0.000 description 3
- 230000002380 cytological effect Effects 0.000 description 3
- 238000000265 homogenisation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000004909 Moisturizer Substances 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 229940070641 chamomile flowers Drugs 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 230000001333 moisturizer Effects 0.000 description 2
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 244000205574 Acorus calamus Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000015763 Artemisia ludoviciana Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000011996 Calamus deerratus Nutrition 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 208000006558 Dental Calculus Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000195955 Equisetum hyemale Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010018276 Gingival bleeding Diseases 0.000 description 1
- 241000608847 Gnaphalium Species 0.000 description 1
- 244000141009 Hypericum perforatum Species 0.000 description 1
- 235000017309 Hypericum perforatum Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- QOSMNYMQXIVWKY-UHFFFAOYSA-N Propyl levulinate Chemical compound CCCOC(=O)CCC(C)=O QOSMNYMQXIVWKY-UHFFFAOYSA-N 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 240000009089 Quercus robur Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910021486 amorphous silicon dioxide Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 210000003298 dental enamel Anatomy 0.000 description 1
- 239000000551 dentifrice Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 239000006153 eosin methylene blue Substances 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 208000011759 gum bleeding Diseases 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000003239 periodontal effect Effects 0.000 description 1
- 210000004261 periodontium Anatomy 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 231100000701 toxic element Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/534—Mentha (mint)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
Definitions
- the present invention relates to the field of dentistry and concerns oral care products containing a stabilizer, a surfactant, an abrasive, a moisturizer, an antiseptic and analgesic component, a solvent, and extracts of medicinal plant materials.
- Patent RU 2203032C1 proposes an oral care composition.
- the components are taken in the following ratio, wt.%:
- Aromatic composition - 0.8 - 2.0;
- SC-CO2-echinacea concentrate - 0.05 - 0.50;
- This dentifrice (toothpaste) has an insufficient therapeutic and prophylactic effect on the tissues and organs of the oral cavity, in particular, it has a delayed anti-inflammatory effect, and is also not effective enough against plaque.
- the objective of the present invention is to create a composition for the treatment of diseases of the oral cavity, care of teeth and oral cavity, showing a high degree of anti-inflammatory, antimicrobial and whitening action.
- Example N1 including components in the following ratio, wt.%:
- Example N2 including components in the following ratio, wt.%:
- Example N3 including components in the following ratio, wt.%:
- the toothpaste has a yellowish color, a pronounced mint smell, a strong mint taste and is a homogeneous jelly-like mass without grains.
- the pH of a 25% aqueous solution of the resulting toothpaste is 8.62.
- Chamomile flower extract has anti-inflammatory and regenerating properties.
- the analgesic property is due to the content of essential oils and flavonoids.
- An extract of chamomile flowers is obtained by extracting dried chamomile flowers (Matricaria recutita) with 70% ethyl alcohol.
- Echinacea purpurea herb extract has immunomodulatory and antimicrobial properties and inhibits the growth of many viruses, fungi, microorganisms, promotes wound healing. Contains sodium, potassium, manganese, iron, copper, nickel and other elements. Increases immunity and is a source of trace elements.
- An extract of Echinacea purpurea herb is obtained by extracting dry Echinacea purpurea herb with 70% ethyl alcohol.
- Salvia officinalis leaf extract has antimicrobial and anti-inflammatory properties and exhibits astringent, disinfectant and phytoncidal properties, actively acting on capillaries, reducing the permeability of the walls and reducing gum bleeding, suppresses gram-positive strains of bacteria.
- An extract of sage officinalis leaves is obtained by extracting dry sage leaves with 70% ethyl alcohol.
- Juniper fruit extract has analgesic and disinfectant properties.
- An extract of juniper fruits is obtained by extracting dry juniper fruits with 70% ethyl alcohol.
- Xanthan gum is a stabilizer, helps to maintain the consistency of toothpaste (binder).
- Cocamidopropyl betaine is a surfactant used as a foam enhancer (detergent).
- Kaolinite clays remove tartar and strengthen enamel (abrasive).
- Sodium bicarbonate has a bleaching effect (abrasive).
- Glycerin gives the paste plasticity, form a consistency, ensures the retention of moisture at a certain level (moisturizer).
- Peppermint essential oil has a cooling effect, has a pleasant smell and is used as a fragrance.
- Purified water is used as a solvent (solvent).
- Dispersion from Latin dispersio - scattering
- Fine grinding of solids or liquids from Latin dispersio - scattering
- Homogenization is a technological process performed on a two- or multi-phase system, during which the degree of heterogeneity in the distribution of chemicals and phases over the volume of the heterophase system is reduced. Determination of microbiological purity and limiting content of toxic elements of this toothpaste was carried out on the basis of the Testing Laboratories of LLP "NUTRITEST". The test results are shown in Table N°l.
- the following method was used: the studied microbial culture was sown on the surface of the nutrient lawn in Petri dishes. Then, disks impregnated with the test base of the therapeutic and prophylactic paste were placed on the surface of the cultures. Petri dishes were placed in a thermostat for incubation for 24 hours at 37°C. After that, the presence or absence of growth of microorganisms was taken into account with an accuracy of 0.1 mm.
- the conducted studies show the following results: the diameter of the zone of inhibition of the growth of microorganisms was in the range from 8.5 to 13.2 mm. Number sensitive strains amounted to 80.4%. The results obtained indicate a significant antibacterial effectiveness of the claimed toothpaste.
- test tubes For immunological studies in patients of the control group and in patients (comparison group), the studied liquid of the oral mucosa was taken into test tubes in an amount of 10 ml.
- cytograms of the epithelium of the oral mucosa of patients revealed a large number of segmented neutrophils, the number of which varies within 59.2 ⁇ 2.6%.
- the number of mononuclear cells increased significantly - 14.1 ⁇ 3.8%.
- the total number of epithelial cells of all types per 1000 cells decreased due to a sharp increase in the number of neutrophils and mononuclear cells.
- the ratio of the number of cells of the 1st and 2nd types to the number of cells of the 3rd and 4th types increased in the cytogram, reaching a value of 0.29 ⁇ 0.03, which is observed during inflammation and indicates the predominance of proliferation processes over the processes differentiation.
- the treatment carried out by new drugs caused changes in the cytological picture of smears-prints in patients with pathology of the periodontium and oral mucosa. Positive dynamics in relation to all indicators, compared with baseline indicators, is presented by statistical processing of the analysis results (P ⁇ 0.001).
- the picture of the cytogram in patients with periodontal pathology differed significantly from the data of the cytogram before treatment: against the background of a decrease in epithelial cells of the 1st and 2nd types, an increase in epithelial cells of the 3rd and 4th types was noted. The number of phage cells, mononuclear cells, and bare-nuclear monocytes decreased.
- stage 1 eosin-methylene blue for 15 minutes.
- Stage 2 using azur-eosin according to Romanovsky for 35 minutes.
- Types I, II, III, and IV epithelial cells, contaminated, phage cells, mononuclear cells, including bare nuclear cells, neutrophils, and dystrophically altered cells per 1000 cells were counted.
- cytological examination of smears with the help of a professional package of statistical programs StatSoft "Statistica - 6"
- digital material was processed, followed by analysis of the data obtained. The significance of differences in mean values was assessed using Student's t-test.
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Medical Informatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Alternative & Traditional Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Birds (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Cosmetics (AREA)
Abstract
Field of use: the invention relates to medicine, and more particularly to dentistry, and has a therapeutic and prophylactic purpose. The proposed therapeutic and prophylactic toothpaste composition contains: 1. wild chamomile flower extract - 0.7 wt%; 2. common sage leaf extract - 0.7 wt%; 3. purple echinacea herb extract 0.7 wt%; 4. juniper berry extract - 0.7 wt%; 5. xanthan gum - 1.5 wt%; 6. cocamidopropyl betaine - 0.5 wt%; 7. kaolinite clays - 15.0 wt%; 8. sodium bicarbonate - 10.0 wt%; 9. glycerine - 17.0 wt%; 10. mint essential oil - 0.9 wt%; 11. purified water - the balance. The compatibility of individual ingredients of the claimed composition provides a synergistic effect that gives rise to novel properties and to a broad range of pharmacological and cosmetic effects, which becomes apparent during use. The toothpaste complies with sanitary rules and norms governing hygienic properties, and has a therapeutic, whitening and anti-inflammatory effect.
Description
Описание изобретения Description of the invention
Настоящее изобретение относится к области стоматологии и касается средств по уходу за полостью рта, содержащей стабилизатор, ПАВ, абразив, увлажнитель, антисептический и обезболивающий компонент, растворитель и экстракты лекарственного растительного сырья. The present invention relates to the field of dentistry and concerns oral care products containing a stabilizer, a surfactant, an abrasive, a moisturizer, an antiseptic and analgesic component, a solvent, and extracts of medicinal plant materials.
В патенте RU 2203032С1 предлагается композиция по уходу за полостью рта. В патенте, для приготовления пасты компоненты берут в следующем соотношении, мас.%:Patent RU 2203032C1 proposes an oral care composition. In the patent, for the preparation of the paste, the components are taken in the following ratio, wt.%:
1. Глицерин дистиллированный - 10,0 - 25,0; 1. Distilled glycerin - 10.0 - 25.0;
2. Мел химически осажденный - 14,0 - 20,0; 2. Chemically precipitated chalk - 14.0 - 20.0;
3. Сорбитол - 12,5 - 25,0; 3. Sorbitol - 12.5 - 25.0;
4. Синтетическая тонкодиспергированная аморфная двуокись кремния - 5,0 - 15,0; 4. Synthetic finely dispersed amorphous silicon dioxide - 5.0 - 15.0;
5. ПЭГ-12 или пропиленгликоль - 2,0 - 10,0; 5. PEG-12 or propylene glycol - 2.0 - 10.0;
6. Натрий карбоксиметилцеллюлоза - 0,5 - 2,0; 6. Sodium carboxymethylcellulose - 0.5 - 2.0;
7. Натрия лаурилсульфат - 1,0 - 2,0; 7. Sodium lauryl sulfate - 1.0 - 2.0;
8. Ароматическая композиция - 0,8 - 2,0; 8. Aromatic composition - 0.8 - 2.0;
9. Натрия бензоат пищевой, или метиловый эфир параоксибензойной кислоты, или пропиловый эфир параоксибензойной кислоты - 0,1 - 0,6; 9. Sodium benzoate food, or methyl ester of paraoxybenzoic acid, or propyl ester of paraoxybenzoic acid - 0.1 - 0.6;
10. Двуокись титана пигментная - 0,3 - 0,6; 10. Pigmentary titanium dioxide - 0.3 - 0.6;
11. Сахарин или сахаринат натрия - 0,08 - 0,20; 11. Saccharin or sodium saccharin - 0.08 - 0.20;
12. СК-С02-концентрат шалфея - 0,05 - 0,50; 12. SK-C02-concentrate of sage - 0.05 - 0.50;
13. СК-С02-концентрат грецкого ореха - 0,05 - 0,50; 13. SC-C02 walnut concentrate - 0.05 - 0.50;
14. СК-С02-концентрат хвоща полевого - 0,05 - 0,50; 14. SK-C02-concentrate of horsetail - 0.05 - 0.50;
15. СК-С02-концентрат эхинацеи - 0,05 - 0,50; 15. SC-CO2-echinacea concentrate - 0.05 - 0.50;
16. СК-С02-концентрат розмарина - 0,05 - 0,50; 16. SC-C02-rosemary concentrate - 0.05 - 0.50;
17. Вода питьевая - остальное. 17. Drinking water - the rest.
Данное средство для чистки зубов (зубная паста) оказывает недостаточное лечебно-профилактическое влияние на ткани и органы полости рта, в частности оказывает замедленное противовоспалительное действие, а также недостаточно эффективна против зубного налета. This dentifrice (toothpaste) has an insufficient therapeutic and prophylactic effect on the tissues and organs of the oral cavity, in particular, it has a delayed anti-inflammatory effect, and is also not effective enough against plaque.
Наиболее близким является патент РФ 2180555, содержащий компоненты в следующей композиции (мас.%): The closest is RF patent 2180555 containing components in the following composition (wt.%):
1. Трава ромашки аптечной - 22,0 - 25,0; 1. Chamomile herb - 22.0 - 25.0;
2. Трава зверобоя продырявленного - 22,0 - 25,0; 2. St. John's wort grass - 22.0 - 25.0;
3. Трава сушеницы топяной - 20,0 - 25,0; 3. Grass cudweed swamp - 20.0 - 25.0;
4. Трава эхинацеи пурпурной - 12,0 - 15,0;
5. Листья шалфея лекарственного - 8,0 - 10,0; 4. Grass Echinacea purpurea - 12.0 - 15.0; 5. Leaves of sage officinalis - 8.0 - 10.0;
6. Кора дуба обыкновенного - 15,0 - 20,0; 6. Common oak bark - 15.0 - 20.0;
7. Корневище аира болотного - 8,0 - 10,0; 7. Rhizome of calamus - 8.0 - 10.0;
8. Вода - остальное. 8. Water - the rest.
Недостатком этого средства является его недостаточное обезболивающее и антисептическое действие. The disadvantage of this remedy is its insufficient analgesic and antiseptic effect.
Решаемой задачей настоящего изобретения является создание состава для лечения заболеваний полости рта, ухода за зубами и полостью рта, проявляющего высокую степень противовоспалительного, антимикробного и отбеливающего действия. The objective of the present invention is to create a composition for the treatment of diseases of the oral cavity, care of teeth and oral cavity, showing a high degree of anti-inflammatory, antimicrobial and whitening action.
Поставленная задача решается подбором состава для профилактики и лечения заболеваний полости рта и ухода за зубами и полостью рта, представленными следующими примерами: The problem is solved by the selection of the composition for the prevention and treatment of diseases of the oral cavity and care of the teeth and oral cavity, represented by the following examples:
Пример N1, включающей компоненты в следующем соотношении, мас.%: Example N1, including components in the following ratio, wt.%:
1. Экстракт цветков ромашки аптечной - 0,7 1. Chamomile flower extract - 0.7
2. Экстракт листьев шалфея лекарственного - 0,7 2. Leaf extract of sage officinalis - 0.7
3. Экстракт травы эхинацеи пурпурной - 0,7 3. Echinacea purpurea herb extract - 0.7
4. Экстракт плодов можжевельника - 0,7 4. Juniper fruit extract - 0.7
5. Ксантановая камедь - 1,5 5. Xanthan gum - 1.5
6. Кокамидопропил бетаин - 0,5 6. Cocamidopropyl betaine - 0.5
7. Каолинитовые глины - 6,0 7. Kaolinite clays - 6.0
8. Натрия бикарбонат - 20,0 8. Sodium bicarbonate - 20.0
9. Глицерин - 20,0 9. Glycerin - 20.0
10. Эфирное масло мяты - 0,9 10. Peppermint essential oil - 0.9
11. Вода очищенная - остальное. 11. Purified water - the rest.
По примеру N 1 получилась жидкая масса, чем в остальных пропорциях. According to example N 1, a liquid mass was obtained than in other proportions.
Пример N2, включающей компоненты в следующем соотношении, мас.%: Example N2, including components in the following ratio, wt.%:
1. Экстракт цветков ромашки аптечной - 0,7 1. Chamomile flower extract - 0.7
2. Экстракт листьев шалфея лекарственного - 0,7 2. Leaf extract of sage officinalis - 0.7
3. Экстракт травы эхинацеи пурпурной - 0,7 3. Echinacea purpurea herb extract - 0.7
4. Экстракт плодов можжевельника - 0,7 4. Juniper fruit extract - 0.7
5. Ксантановая камедь - 1,5 5. Xanthan gum - 1.5
6. Кокамидопропил бетаин - 0,5 6. Cocamidopropyl betaine - 0.5
7. Каолинитовые глины - 15,0 7. Kaolinite clays - 15.0
8. Натрия бикарбонат - 10,0 8. Sodium bicarbonate - 10.0
9. Глицерин - 17,0
10. Эфирное масло мяты - 0,9 9. Glycerin - 17.0 10. Peppermint essential oil - 0.9
11. Вода очищенная - остальное. 11. Purified water - the rest.
По примеру N2, получилась неоднородная творожная масса. Following the example of N2, a heterogeneous curd mass was obtained.
Пример N3, включающей компоненты в следующем соотношении, мас.%: Example N3, including components in the following ratio, wt.%:
1. Экстракт цветков ромашки аптечной - 0,07 1. Chamomile flower extract - 0.07
2. Экстракт листьев шалфея лекарственного - 0,07 2. Leaf extract of sage officinalis - 0.07
3. Экстракт травы эхинацеи пурпурной - 0,07 3. Echinacea purpurea herb extract - 0.07
4. Экстракт плодов можжевельника - 0,07 4. Juniper fruit extract - 0.07
5. Ксантановая камедь - 1,5 5. Xanthan gum - 1.5
6. Кокамидопропил бетаин - 0,5 6. Cocamidopropyl betaine - 0.5
7. Каолинитовые глины - 10,0 7. Kaolinite clays - 10.0
8. Натрия бикарбонат - 10,0 8. Sodium bicarbonate - 10.0
9. Глицерин - 15,0 9. Glycerin - 15.0
10. Эфирное масло мяты - 0,9 10. Peppermint essential oil - 0.9
11. Вода очищенная - остальное. 11. Purified water - the rest.
По примеру N3, зубная паста имеет желтоватый цвет, ярко-выраженный мятный запах, сильный мятный вкус и представляет однородную желеобразную массу без крупинок. pH 25 % водного раствора полученной зубной пасты составляет 8,62. According to example N3, the toothpaste has a yellowish color, a pronounced mint smell, a strong mint taste and is a homogeneous jelly-like mass without grains. The pH of a 25% aqueous solution of the resulting toothpaste is 8.62.
Свойства компонентов настоящей зубной пасты: Properties of the components of this toothpaste:
Экстракт цветков ромашки аптечной обладает противовоспалительным и регенерирующим свойствами. Обезболивающее свойство обусловливается содержанием эфирных масел и флавоноидов. Получают экстракт цветков ромашки аптечной путем экстракции сухих цветков ромашки аптечной (Matricaria recutita) 70 % этиловым спиртом. Chamomile flower extract has anti-inflammatory and regenerating properties. The analgesic property is due to the content of essential oils and flavonoids. An extract of chamomile flowers is obtained by extracting dried chamomile flowers (Matricaria recutita) with 70% ethyl alcohol.
Экстракт травы эхинацеи пурпурной обладает иммуномодулирующим и антимикробным свойствами и подавляет рост многих вирусов, грибков, микроорганизмов, способствует заживлению ран. Содержит натрий, калий, марганец, железо, медь, никель и другие элементы. Повышает иммунитет и является источником микроэлементов. Получают экстракт травы эхинацеи пурпурной путем экстракции сухой травы эхинацеи пурпурной 70 % этиловым спиртом. Echinacea purpurea herb extract has immunomodulatory and antimicrobial properties and inhibits the growth of many viruses, fungi, microorganisms, promotes wound healing. Contains sodium, potassium, manganese, iron, copper, nickel and other elements. Increases immunity and is a source of trace elements. An extract of Echinacea purpurea herb is obtained by extracting dry Echinacea purpurea herb with 70% ethyl alcohol.
Экстракт листьев шалфея лекарственного обладает антимикробным и противовоспалительным свойствами и проявляет вяжущие, дезинфицирующие и фитонцидные свойства, активно действуя на капилляры, снижая проницаемость стенок и уменьшая кровоточивость десен, подавляет граммположительные штаммы бактерий. Получают экстракт листьев шалфея лекарственного путем экстракции сухих листьев шалфея лекарственного 70 % этиловым спиртом.
Экстракт плодов можжевельника обладает болеутоляющим и дезинфицирующим свойствами. Получают экстракт плодов можжевельника путем экстракции сухих плодов можжевельника 70 % этиловым спиртом. Salvia officinalis leaf extract has antimicrobial and anti-inflammatory properties and exhibits astringent, disinfectant and phytoncidal properties, actively acting on capillaries, reducing the permeability of the walls and reducing gum bleeding, suppresses gram-positive strains of bacteria. An extract of sage officinalis leaves is obtained by extracting dry sage leaves with 70% ethyl alcohol. Juniper fruit extract has analgesic and disinfectant properties. An extract of juniper fruits is obtained by extracting dry juniper fruits with 70% ethyl alcohol.
Ксантановая камедь является стабилизатором, способствуют сохранению консистенции зубной пасты (связывающее вещество). Xanthan gum is a stabilizer, helps to maintain the consistency of toothpaste (binder).
Кокамидопропил бетаин является поверхностно-активным веществом, применяется в качестве усилителя пены (детергент). Cocamidopropyl betaine is a surfactant used as a foam enhancer (detergent).
Каолинитовые глины снимают зубные камни и укрепляет эмаль (абразив). Kaolinite clays remove tartar and strengthen enamel (abrasive).
Натрия бикарбонат оказывает отбеливающее воздействие (абразив). Sodium bicarbonate has a bleaching effect (abrasive).
Глицерин придает пасте пластичность, формируют консистенцию, обеспечивает удержание влаги на определенном уровне (увлажнитель). Glycerin gives the paste plasticity, form a consistency, ensures the retention of moisture at a certain level (moisturizer).
Эфирное масло мяты обладают охлаждающим эффектом, имеет приятный запах и применяется в качестве ароматизатора. Peppermint essential oil has a cooling effect, has a pleasant smell and is used as a fragrance.
Вода очищенная применяется в качестве растворителя (растворитель). Purified water is used as a solvent (solvent).
Способ получения заявленной композиции: Method for obtaining the claimed composition:
1. Смешивание установленных количеств натрия бикарбоната, ксантановой камеди, диспергирование (10 мин); 1. Mixing prescribed amounts of sodium bicarbonate, xanthan gum, dispersion (10 min);
2. Добавление установленного количества воды, глицерина и получения геля (15-20 мин);2. Adding a specified amount of water, glycerin and obtaining a gel (15-20 min);
3. Введение установленного количества каолинитовых глин, гомогенизация (25-30 мин);3. Introduction of a fixed amount of kaolinite clays, homogenization (25-30 min);
4. Введение установленных количеств экстрактов ромашки аптечной, шалфея лекарственного, эхинацеи пурпурной и перемешивают до полного растворения. 4. The introduction of established amounts of extracts of chamomile, medicinal sage, echinacea purpurea and mix until completely dissolved.
5. Введение установленного количества кокамидопропил бетаина, гомогенизация (20 мин); 5. Introduction of a prescribed amount of cocamidopropyl betaine, homogenization (20 min);
6. Созревание пасты в течение 5 суток. 6. Maturation of the paste for 5 days.
7. Расфасовка зубной пасты по тубам. 7. Packaging of toothpaste in tubes.
Примечание: Note:
Диспергирование (от лат. dispersio - рассеяние) - тонкое измельчение твёрдых веществ или жидкостей. Dispersion (from Latin dispersio - scattering) - fine grinding of solids or liquids.
Гомогенизация (от греч. όmogenήV - однородный) - технологический процесс, производимый над двух - или многофазной системой, в ходе которого уменьшается степень неоднородности распределения химических веществ и фаз по объёму гетерофазной системы.
Определение микробиологической чистоты и предельного содержания токсичных элементов настоящей зубной пасты производилось на базе Испытательной лабораторий ТОО «НУТРИТЕСТ». Результаты испытаний приведены в таблице N°l. Homogenization (from the Greek όmogenήV - homogeneous) is a technological process performed on a two- or multi-phase system, during which the degree of heterogeneity in the distribution of chemicals and phases over the volume of the heterophase system is reduced. Determination of microbiological purity and limiting content of toxic elements of this toothpaste was carried out on the basis of the Testing Laboratories of LLP "NUTRITEST". The test results are shown in Table N°l.
Таблица 1 - Определение показателей качества зубной пасты
Table 1 - Definition of toothpaste quality indicators
Изучение микробиологической активности настоящей зубной пасты производилось на базе Института им. Б. Атчабарова в опытах in vitro на основании динамики роста патогенных микроорганизмов методом диффузии в агар. В качестве патогенных микроорганизмов были взяты музейные штаммы: Pseudomonas aeruginosa, Staphylococcus aureus (стафилококк золотистый), Esherichia сой (кишечная палочка), Candida (дрожжеподобный гриб). В качестве метода исследования использовался диско- диффузионный метод. В качестве контроля роста бактерий использовали образцы, содержащие среду и тестируемые штаммы без стандартного лекарственного препарата. Применялась следующая методика: на поверхность питательного газона в чашках Петри засевалась исследуемая культура микроорганизмов. Затем на поверхности культур накладывали диски, пропитанные исследуемой основой лечебно-профилактической пасты. Чашки Петри помещали в термостат для инкубирования на 24 часа при 37 °С. После этого учитывали наличие или отсутствие роста микроорганизмов с точностью до 0,1 мм. Проведенные исследования показывают следующие результаты: диаметр зоны задержки роста микроорганизмов находился в интервале от 8,5 до 13,2 мм. Число
чувствительных штаммов составило 80,4 %. Полученные результаты свидетельствуют о значительной антибактериальной эффективности заявленной зубной пасты. The study of the microbiological activity of this toothpaste was carried out on the basis of the Institute. B. Atchabarova in experiments in vitro based on the dynamics of growth of pathogenic microorganisms by the method of diffusion into agar. Museum strains were taken as pathogenic microorganisms: Pseudomonas aeruginosa, Staphylococcus aureus (staphylococcus aureus), Esherichia soy (E. coli), Candida (yeast-like fungus). The disk diffusion method was used as a research method. As a control for bacterial growth, samples containing the medium and test strains without a standard drug were used. The following method was used: the studied microbial culture was sown on the surface of the nutrient lawn in Petri dishes. Then, disks impregnated with the test base of the therapeutic and prophylactic paste were placed on the surface of the cultures. Petri dishes were placed in a thermostat for incubation for 24 hours at 37°C. After that, the presence or absence of growth of microorganisms was taken into account with an accuracy of 0.1 mm. The conducted studies show the following results: the diameter of the zone of inhibition of the growth of microorganisms was in the range from 8.5 to 13.2 mm. Number sensitive strains amounted to 80.4%. The results obtained indicate a significant antibacterial effectiveness of the claimed toothpaste.
Для иммунологических исследований у пациентов контрольной группы и у больных (группа сравнения) была взята в пробирки исследуемая жидкость слизистой оболочки полости рта в количестве 10 мл. For immunological studies in patients of the control group and in patients (comparison group), the studied liquid of the oral mucosa was taken into test tubes in an amount of 10 ml.
При патологии в цитограммах эпителия слизистой оболочки рта больных выявлялось большое количество сегментоядерных нейтрофилов, количество которых варьирует в пределах 59,2 ± 2,6 %. Значительно выросло количество мононуклеаров - 14,1 ± 3,8 %. Суммарное количество эпителиальных клеток всех типов на 1000 клеток снижалось за счет резкого увеличения числа нейтрофилов и мононуклеаров. При этом в цитограмме возрастало отношение количества клеток 1-го и 2-го типов к количеству клеток 3-го и 4-го типов, достигая величины 0,29 ±0,03, что наблюдается при воспалении и свидетельствует о преобладании процессов пролиферации над процессами дифференцировки. Лечение, проведенное новыми лекарственными средствами, вызывало изменения в цитологической картине мазков-отпечатков у больных с патологией пародонта и слизистой оболочки полости рта. Положительная динамика в отношении всех показателей, по сравнению с исходными показателями, представлена по статистической обработке результатов анализа (Р < 0,001). Картина цитограммы у больных с патологией пародонта существенно отличалась от данных цитограммы до лечения: на фоне понижения эпителиальных клеток 1-го и 2-го типов, отмечалось повышение эпителиальных клеток 3-го и 4-го типов. Уменьшалось количество фагирующих клеток, мононуклеаров, голоядерных моноцитов. Но наиболее значимым было понижение сегментоядерных нейтрофилов - почти в 1,5 раза, что свидетельствует о положительной динамике профилактики. Положительная картина цитограмм отмечалась у больных с патологией слизистой оболочки рта: наблюдалось резкое понижение количества сегментоядерных нейтрофилов, мононуклеаров, фагирующих клеток и контаминированных эпителиоцитов 2-го, 3-го и 4-го типов, но они не достигали нормы. Наглядно наблюдалась тенденция повышения эпителиальных клеток 3-го и 4-го типов. In pathology, cytograms of the epithelium of the oral mucosa of patients revealed a large number of segmented neutrophils, the number of which varies within 59.2 ± 2.6%. The number of mononuclear cells increased significantly - 14.1 ± 3.8%. The total number of epithelial cells of all types per 1000 cells decreased due to a sharp increase in the number of neutrophils and mononuclear cells. At the same time, the ratio of the number of cells of the 1st and 2nd types to the number of cells of the 3rd and 4th types increased in the cytogram, reaching a value of 0.29 ± 0.03, which is observed during inflammation and indicates the predominance of proliferation processes over the processes differentiation. The treatment carried out by new drugs caused changes in the cytological picture of smears-prints in patients with pathology of the periodontium and oral mucosa. Positive dynamics in relation to all indicators, compared with baseline indicators, is presented by statistical processing of the analysis results (P < 0.001). The picture of the cytogram in patients with periodontal pathology differed significantly from the data of the cytogram before treatment: against the background of a decrease in epithelial cells of the 1st and 2nd types, an increase in epithelial cells of the 3rd and 4th types was noted. The number of phage cells, mononuclear cells, and bare-nuclear monocytes decreased. But the most significant was the decrease in segmented neutrophils - almost 1.5 times, which indicates a positive trend in prevention. A positive picture of cytograms was noted in patients with pathology of the oral mucosa: there was a sharp decrease in the number of segmented neutrophils, mononuclear cells, phage cells and contaminated epithelial cells of the 2nd, 3rd and 4th types, but they did not reach the norm. There was a clear tendency to increase epithelial cells of the 3rd and 4th types.
Для цитологических исследований применялась методика Романовского - Гимза. Окрашивание проводилось различными смешанными индикаторами в 2 этапа: 1 этап - эозин-метиленовый синий в течение 15 минут. На этом этапе окрашиваются ядра клеток. 2 этап - с помощью азур-эозин по Романовскому в течение 35 минут. Подсчитывали эпителиальные клетки I, II, III и IV типов, контаминированные, фагирующие клетки, мононуклеары, в том числе голоядерные, нейтрофилы, а также дистрофически измененные клетки из расчета на 1000 клеток. При цитологическом исследовании мазков
с помощью профессионального пакета статистических программ StatSoft «Statistica - 6» проводилась обработка цифрового материала, последующий анализ полученных данных. Оценку достоверности различий средних значении проводили с использованием критерия Стьюдента. For cytological studies, the Romanovsky-Giemsa method was used. Staining was carried out with various mixed indicators in 2 stages: stage 1 - eosin-methylene blue for 15 minutes. At this stage, the nuclei of the cells are stained. Stage 2 - using azur-eosin according to Romanovsky for 35 minutes. Types I, II, III, and IV epithelial cells, contaminated, phage cells, mononuclear cells, including bare nuclear cells, neutrophils, and dystrophically altered cells per 1000 cells were counted. During cytological examination of smears with the help of a professional package of statistical programs StatSoft "Statistica - 6", digital material was processed, followed by analysis of the data obtained. The significance of differences in mean values was assessed using Student's t-test.
Результаты лабораторных испытаний показывает, что заявленная зубная паста обладает противовоспалительным, антибактериальным, ранозаживляющим и иммуностимулирующим действиями.
The results of laboratory tests show that the claimed toothpaste has anti-inflammatory, antibacterial, wound healing and immunostimulating effects.
Claims
1. Экстракт цветков ромашки аптечной - 0,07 1. Chamomile flower extract - 0.07
2. Экстракт листьев шалфея лекарственного - 0,07 2. Leaf extract of sage officinalis - 0.07
3. Экстракт травы эхинацеи пурпурной - 0,07 3. Echinacea purpurea herb extract - 0.07
4. Экстракт плодов можжевельника - 0,07 4. Juniper fruit extract - 0.07
5. Ксантановая камедь - 1,5 5. Xanthan gum - 1.5
6. Кокамидопропил бетаин - 0,5 6. Cocamidopropyl betaine - 0.5
7. Каолинитовые глины - 10,0 7. Kaolinite clays - 10.0
8. Натрия бикарбонат - 10,0 8. Sodium bicarbonate - 10.0
9. Глицерин - 15,0 9. Glycerin - 15.0
10. Эфирное масло мяты - 0,9 10. Peppermint essential oil - 0.9
11. Вода очищенная - остальное.
11. Purified water - the rest.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2808887C1 (en) * | 2023-05-29 | 2023-12-05 | Федеральное государственное автономное образовательное учреждение высшего образования "Белгородский государственный национальный исследовательский университет" (НИУ "БелГУ") | Therapeutic composition for treatment of periodontal tissue |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2014105805A (en) * | 2013-06-07 | 2015-08-27 | Валерий Валентинович Правдин | MULTIFUNCTIONAL DENTAL PASTE |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2014105805A (en) * | 2013-06-07 | 2015-08-27 | Валерий Валентинович Правдин | MULTIFUNCTIONAL DENTAL PASTE |
Non-Patent Citations (1)
| Title |
|---|
| SCHEMEHORN BR1, MOORE MH, PUTT MS.: "Abrasion, polishing, and stain removal characteristics of various commercial dentifrices in vitro.", JOURNAL OF CLINICAL DENTISTRY., PROFESSIONAL AUDIENCE COMMUNICATIONS, YARDLEY, PA., US, vol. 22, no. 1, 1 January 2011 (2011-01-01), US , pages 11 - 18, XP009188828, ISSN: 0895-8831 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2808887C1 (en) * | 2023-05-29 | 2023-12-05 | Федеральное государственное автономное образовательное учреждение высшего образования "Белгородский государственный национальный исследовательский университет" (НИУ "БелГУ") | Therapeutic composition for treatment of periodontal tissue |
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