WO2023240165A3 - Methods and apparatus for a twenty-five-color fluorescence-based assay and flow cytometry panel - Google Patents

Methods and apparatus for a twenty-five-color fluorescence-based assay and flow cytometry panel Download PDF

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Publication number
WO2023240165A3
WO2023240165A3 PCT/US2023/068101 US2023068101W WO2023240165A3 WO 2023240165 A3 WO2023240165 A3 WO 2023240165A3 US 2023068101 W US2023068101 W US 2023068101W WO 2023240165 A3 WO2023240165 A3 WO 2023240165A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorochromes
flow cytometry
fluorochrome
twenty
methods
Prior art date
Application number
PCT/US2023/068101
Other languages
French (fr)
Other versions
WO2023240165A2 (en
Inventor
Lily PARK
Maria JAIMES
Original Assignee
Cytek Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytek Biosciences, Inc. filed Critical Cytek Biosciences, Inc.
Priority to PCT/US2023/068101 priority Critical patent/WO2023240165A2/en
Publication of WO2023240165A2 publication Critical patent/WO2023240165A2/en
Publication of WO2023240165A3 publication Critical patent/WO2023240165A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/23—Clustering techniques
    • G06F18/231—Hierarchical techniques, i.e. dividing or merging pattern sets so as to obtain a dendrogram
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data

Abstract

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with four excitation lasers and forty-eight color detectors. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
PCT/US2023/068101 2022-06-07 2023-06-07 Methods and apparatus for a twenty-five-color fluorescence-based assay and flow cytometry panel WO2023240165A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/US2023/068101 WO2023240165A2 (en) 2022-06-07 2023-06-07 Methods and apparatus for a twenty-five-color fluorescence-based assay and flow cytometry panel

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63/336,010 2022-06-07
PCT/US2023/068101 WO2023240165A2 (en) 2022-06-07 2023-06-07 Methods and apparatus for a twenty-five-color fluorescence-based assay and flow cytometry panel

Publications (2)

Publication Number Publication Date
WO2023240165A2 WO2023240165A2 (en) 2023-12-14
WO2023240165A3 true WO2023240165A3 (en) 2024-03-14

Family

ID=89119137

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/068101 WO2023240165A2 (en) 2022-06-07 2023-06-07 Methods and apparatus for a twenty-five-color fluorescence-based assay and flow cytometry panel

Country Status (1)

Country Link
WO (1) WO2023240165A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220082488A1 (en) * 2020-06-26 2022-03-17 Cytek Biosciences, Inc. Methods of forming multi-color fluorescence-based flow cytometry panel

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220082488A1 (en) * 2020-06-26 2022-03-17 Cytek Biosciences, Inc. Methods of forming multi-color fluorescence-based flow cytometry panel

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FERRER‐FONT LAURA, PELLEFIGUES CHRISTOPHE, MAYER JOHANNES U., SMALL SAM J., JAIMES MARIA C., PRICE KYLIE M.: "Panel Design and Optimization for High‐Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry", CURRENT PROTOCOLS IN CYTOMETRY, JOHN WILEY & SONS, INC., US, vol. 92, no. 1, 1 March 2020 (2020-03-01), US , pages e70 - e70-25, XP009553767, ISSN: 1934-9297, DOI: 10.1002/cpcy.70 *

Also Published As

Publication number Publication date
WO2023240165A2 (en) 2023-12-14

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