WO2023234891A2 - Novel acetylcholinesterase inhibitors - Google Patents
Novel acetylcholinesterase inhibitors Download PDFInfo
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- WO2023234891A2 WO2023234891A2 PCT/TR2023/050100 TR2023050100W WO2023234891A2 WO 2023234891 A2 WO2023234891 A2 WO 2023234891A2 TR 2023050100 W TR2023050100 W TR 2023050100W WO 2023234891 A2 WO2023234891 A2 WO 2023234891A2
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- piperazine
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- 239000000544 cholinesterase inhibitor Substances 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 7
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 claims description 73
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims description 68
- -1 aryl piperidine Chemical compound 0.000 claims description 34
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 26
- 208000024827 Alzheimer disease Diseases 0.000 claims description 15
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 13
- 235000015320 potassium carbonate Nutrition 0.000 claims description 11
- 239000002904 solvent Substances 0.000 claims description 10
- 229940100578 Acetylcholinesterase inhibitor Drugs 0.000 claims description 8
- 229940073608 benzyl chloride Drugs 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- NQRYJNQNLNOLGT-UHFFFAOYSA-N tetrahydropyridine hydrochloride Natural products C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 claims description 5
- AGEZXYOZHKGVCM-UHFFFAOYSA-N benzyl bromide Chemical compound BrCC1=CC=CC=C1 AGEZXYOZHKGVCM-UHFFFAOYSA-N 0.000 claims description 4
- XJTQJERLRPWUGL-UHFFFAOYSA-N iodomethylbenzene Chemical compound ICC1=CC=CC=C1 XJTQJERLRPWUGL-UHFFFAOYSA-N 0.000 claims description 4
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 4
- KCXMKQUNVWSEMD-UHFFFAOYSA-N benzyl chloride Chemical group ClCC1=CC=CC=C1 KCXMKQUNVWSEMD-UHFFFAOYSA-N 0.000 claims description 3
- 229940088679 drug related substance Drugs 0.000 claims description 3
- IQXXEPZFOOTTBA-UHFFFAOYSA-N 1-benzylpiperazine Chemical class C=1C=CC=CC=1CN1CCNCC1 IQXXEPZFOOTTBA-UHFFFAOYSA-N 0.000 claims description 2
- 150000005524 benzylchlorides Chemical class 0.000 claims description 2
- 229940068031 l-formula Drugs 0.000 claims 3
- 239000008186 active pharmaceutical agent Substances 0.000 claims 1
- IAZDPXIOMUYVGZ-WFGJKAKNSA-N Dimethyl sulfoxide Chemical compound [2H]C([2H])([2H])S(=O)C([2H])([2H])[2H] IAZDPXIOMUYVGZ-WFGJKAKNSA-N 0.000 description 88
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 48
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 23
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 22
- 238000005160 1H NMR spectroscopy Methods 0.000 description 21
- ADEBPBSSDYVVLD-UHFFFAOYSA-N donepezil Chemical compound O=C1C=2C=C(OC)C(OC)=CC=2CC1CC(CC1)CCN1CC1=CC=CC=C1 ADEBPBSSDYVVLD-UHFFFAOYSA-N 0.000 description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
- 230000003993 interaction Effects 0.000 description 11
- 229960003530 donepezil Drugs 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- IANQTJSKSUMEQM-UHFFFAOYSA-N 1-benzofuran Chemical compound C1=CC=C2OC=CC2=C1 IANQTJSKSUMEQM-UHFFFAOYSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 102100033639 Acetylcholinesterase Human genes 0.000 description 5
- 108010022752 Acetylcholinesterase Proteins 0.000 description 5
- 229940022698 acetylcholinesterase Drugs 0.000 description 5
- 125000003118 aryl group Chemical group 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- ASUTZQLVASHGKV-JDFRZJQESA-N galanthamine Chemical compound O1C(=C23)C(OC)=CC=C2CN(C)CC[C@]23[C@@H]1C[C@@H](O)C=C2 ASUTZQLVASHGKV-JDFRZJQESA-N 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 238000001308 synthesis method Methods 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000010992 reflux Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 208000037259 Amyloid Plaque Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- XSVMFMHYUFZWBK-NSHDSACASA-N Rivastigmine Chemical compound CCN(C)C(=O)OC1=CC=CC([C@H](C)N(C)C)=C1 XSVMFMHYUFZWBK-NSHDSACASA-N 0.000 description 2
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 229960003980 galantamine Drugs 0.000 description 2
- ASUTZQLVASHGKV-UHFFFAOYSA-N galanthamine hydrochloride Natural products O1C(=C23)C(OC)=CC=C2CN(C)CCC23C1CC(O)C=C2 ASUTZQLVASHGKV-UHFFFAOYSA-N 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- SNWQUNCRDLUDEX-UHFFFAOYSA-N inden-1-one Chemical group C1=CC=C2C(=O)C=CC2=C1 SNWQUNCRDLUDEX-UHFFFAOYSA-N 0.000 description 2
- CKJNUZNMWOVDFN-UHFFFAOYSA-N methanone Chemical compound O=[CH-] CKJNUZNMWOVDFN-UHFFFAOYSA-N 0.000 description 2
- 238000000302 molecular modelling Methods 0.000 description 2
- 229960004136 rivastigmine Drugs 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- UIXIUTHWAOTWJY-UHFFFAOYSA-N (4-fluorophenyl)-(6-methoxy-1-benzofuran-2-yl)methanone Chemical compound O1C2=CC(OC)=CC=C2C=C1C(=O)C1=CC=C(F)C=C1 UIXIUTHWAOTWJY-UHFFFAOYSA-N 0.000 description 1
- SFVGCTLMFVDKTP-UHFFFAOYSA-N 1-benzofuran-2-yl-(4-fluorophenyl)methanone Chemical compound C1=CC(F)=CC=C1C(=O)C1=CC2=CC=CC=C2O1 SFVGCTLMFVDKTP-UHFFFAOYSA-N 0.000 description 1
- ZJFWCELATJMDNO-UHFFFAOYSA-N 2-bromo-1-(4-fluorophenyl)ethanone Chemical compound FC1=CC=C(C(=O)CBr)C=C1 ZJFWCELATJMDNO-UHFFFAOYSA-N 0.000 description 1
- SMQUZDBALVYZAC-HOSYLAQJSA-N 2-hydroxybenzaldehyde Chemical class OC1=CC=CC=C1[13CH]=O SMQUZDBALVYZAC-HOSYLAQJSA-N 0.000 description 1
- 125000006283 4-chlorobenzyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1Cl)C([H])([H])* 0.000 description 1
- 108010053652 Butyrylcholinesterase Proteins 0.000 description 1
- 102100032404 Cholinesterase Human genes 0.000 description 1
- 102000003914 Cholinesterases Human genes 0.000 description 1
- 108090000322 Cholinesterases Proteins 0.000 description 1
- GSNUFIFRDBKVIE-UHFFFAOYSA-N DMF Natural products CC1=CC=C(C)O1 GSNUFIFRDBKVIE-UHFFFAOYSA-N 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 208000009668 Neurobehavioral Manifestations Diseases 0.000 description 1
- PIJVFDBKTWXHHD-UHFFFAOYSA-N Physostigmine Natural products C12=CC(OC(=O)NC)=CC=C2N(C)C2C1(C)CCN2C PIJVFDBKTWXHHD-UHFFFAOYSA-N 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- HHRFWSALGNYPHA-UHFFFAOYSA-N [N].C1CNCCN1 Chemical group [N].C1CNCCN1 HHRFWSALGNYPHA-UHFFFAOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 208000006218 bradycardia Diseases 0.000 description 1
- 230000036471 bradycardia Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000001364 causal effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229940048961 cholinesterase Drugs 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 150000004677 hydrates Chemical class 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000012866 low blood pressure Diseases 0.000 description 1
- 230000002132 lysosomal effect Effects 0.000 description 1
- BUGYDGFZZOZRHP-UHFFFAOYSA-N memantine Chemical compound C1C(C2)CC3(C)CC1(C)CC2(N)C3 BUGYDGFZZOZRHP-UHFFFAOYSA-N 0.000 description 1
- 229960004640 memantine Drugs 0.000 description 1
- 206010027175 memory impairment Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 230000003955 neuronal function Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000006186 oral dosage form Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229960001697 physostigmine Drugs 0.000 description 1
- PIJVFDBKTWXHHD-HIFRSBDPSA-N physostigmine Chemical compound C12=CC(OC(=O)NC)=CC=C2N(C)[C@@H]2[C@@]1(C)CCN2C PIJVFDBKTWXHHD-HIFRSBDPSA-N 0.000 description 1
- 235000011181 potassium carbonates Nutrition 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000012453 solvate Substances 0.000 description 1
- 238000005556 structure-activity relationship Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000016978 synaptic transmission, cholinergic Effects 0.000 description 1
- 229960001685 tacrine Drugs 0.000 description 1
- YLJREFDVOIBQDA-UHFFFAOYSA-N tacrine Chemical compound C1=CC=C2C(N)=C(CCCC3)C3=NC2=C1 YLJREFDVOIBQDA-UHFFFAOYSA-N 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/4965—Non-condensed pyrazines
- A61K31/497—Non-condensed pyrazines containing further heterocyclic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/77—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D307/78—Benzo [b] furans; Hydrogenated benzo [b] furans
- C07D307/79—Benzo [b] furans; Hydrogenated benzo [b] furans with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to carbon atoms of the hetero ring
- C07D307/80—Radicals substituted by oxygen atoms
Definitions
- the present invention relates to novel acetylcholinesterase inhibitors, methods of preparing acetylcholinesterase inhibitors of the invention, and pharmaceutical compositions comprising the said acetylcholinesterase inhibitors.
- Alzheimer's disease is a neurodegenerative disorder that causes cognitive symptoms such as forgetfulness and behavioral disorders as a result of impaired neuronal function in the brain.
- the disease has not yet been fully resolved and therefore treatment is palliative.
- theories such as 0- amyloid plaques, senile plaques, lysosomal dysfunction, and impaired cholinergic transmission explain the basis and symptoms of the disease. All these theories have been clearly shown to have a causal or consequential role in the disease. Therefore, drug development efforts in this area are mainly focused on targeting these pathways to develop effective small molecules.
- Physostigmine a natural alkaloid isolated in the late 19th century, was the main drug used to treat Alzheimer's disease.
- tacrine was previously developed for respiratory diseases, it was later used to treat Alzheimer's disease. Rivastigmine, galantamine, and donepezil were subsequently approved. Today, donepezil, memantine, galantamine, and rivastigmine are the main drugs prescribed in the treatment of mild to moderate AD. All these drugs are reported favorably in terms of efficacy and side effects. Among them, donepezil shows the highest affinity at low micromolar levels but also has significant side effects such as extrapyramidal effects, low blood pressure, severe vomiting, bradycardia, and arrhythmia.
- An object of the invention is to develop new acetylcholinesterase inhibitor molecules suitable for use in the treatment of Alzheimer's disease.
- Another object of the invention is to develop new molecules that have the potential to be used in the treatment of Alzheimer's disease and preferably have an acetylcholinesterase inhibitor effect.
- the inventors have determined that the new molecules shown in Formula I below exhibit high acetylcholinesterase inhibitor activity.
- the present invention relates to novel acetylcholinesterase derivatives molecules with potential for use in the treatment of Alzheimer's disease, and the inventive molecules are shown in Formula I below, wherein; X: -H, -OCH 3
- Y -H, -Cl, OCH 3 or X and Y can be -O-CH2-O- forming a combined ring and selected from
- T -H, -OCH 3 R: -H, 2-CH 3 , 3-CH 3 , 4-CH 3 , 2-OCH 3 , 3-OCH 3 , 4-OCH 3 , 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO 2 , 3-NO2, 4-NO2, n: 0 or 1.
- the molecules according to the invention are shown in Formula 1.1-1.95 in a preferred embodiment of the invention, and the variables X, Y, Z, T, R, and n of the said molecules are given in the table below (Table 1).
- a preferred embodiment of the invention relates to pharmaceutically acceptable derivatives of molecules represented by Formula I and/or Formula 1.1 - Formula 1.95.
- the pharmaceutically acceptable derivatives mentioned herein may be salts, solvates, esters, hydrates of the molecules indicated by Formula I or Formula 1.1 - Formula 1.95.
- the phrase "pharmaceutically acceptable derivatives" in this application refers to chemical or physical modifications to increase the solubility or facilitate the formulation or increase the bioavailability of molecules shown in Formula I and/or Formula 1.1 - Formula 1.95 that do not result in a change in the therapeutic activity of the molecule.
- An embodiment of the invention relates to novel acetylcholinesterase inhibitor derivative molecules suitable for use in the treatment of Alzheimer's disease, and the molecules of the invention are denoted by Formula 1.1, Formula 1.2, Formula 1.3, Formula 1.4, Formula 1.5, Formula 1.6, Formula 1.7, Formula 1.8, Formula 1.9, Formula 1.10, Formula 1.11, Formula 1.12, Formula 1.13, Formula 1.14, Formula 1.15, Formula 1.16, Formula 1.17, Formula 1.18, Formula 1.19, Formula 1.20.
- An embodiment of the invention relates to novel acetylcholinesterase inhibitor derivative molecules suitable for use in the treatment of Alzheimer's disease, and the molecules of the invention are denoted by Formula 1.1, Formula 1.2, Formula 1.3, Formula 1.4, Formula 1.5, Formula 1.7, Formula 1.8, Formula 1.9, Formula 1.10, Formula 1.11, Formula 1.12, Formula 1.13, Formula 1.14, Formula 1.15, Formula 1.19, Formula 1.20.
- An embodiment of the invention relates to novel acetylcholinesterase inhibitor-derived molecules suitable for use in the treatment of Alzheimer's disease, and the molecules of the invention are denoted by Formula 1.1, Formula 1.2, Formula 1.3, Formula 1.4, Formula 1.5, Formula 1.7, Formula 1.8, Formula 1.9, Formula 1.10, Formula 1.11, Formula 1.12, Formula 1.13, Formula 1.14, Formula 1.15, Formula 1.19, Formula 1.20, Formula 1.21, Formula 1.22, Formula 1.23, Formula 1.24, Formula 1.25, Formula 1.26, Formula 1.27, Formula 1.28, Formula 1.29, Formula 1.30, Formula 1.31, Formula 1.32, Formula 1.32, Formula 1.33, Formula 1.34, Formula 1.35, Formula 1.36, Formula 1.37, Formula 1.38, Formula 1.39, Formula 1.40, Formula 1.41, Formula 1.42, Formula 1.43, Formula 1.44, Formula 1.45, Formula 1.46, Formula 1.47, Formula 1.48, Formula 1.49, Formula 1.50, Formula 1.51, Formula 1.52, Formula 1.53, Formula 1.54, Formula 1.55, Formula 1.56, Formula 1.57,
- a further embodiment of the invention relates to pharmaceutical compositions comprising molecules indicated by Formula I and/or Formula 1.1 - Formula 1.95.
- compositions according to the invention may be formulated in oral dosage forms.
- pharmaceutically acceptable excipients may be used in addition to the molecules shown in Formula I and/or Formula 1.1 - Formula 1.95 used as drug substances.
- excipient refers to substances that are used to formulate drug substances according to the invention and have no therapeutic efficacy.
- the said excipients can be selected from agents known to be used in pharmaceutical technology.
- the invention relates to a method (Method 1) for the synthesis of the molecules indicated by formula I, preferably formula 1.1, formula 1.2, formula 1.3, formula 1.4, formula 1.5, formula 1.6, formula 1.7, formula 1.8, formula 1.9, formula 1.10, formula 1.11, formula 1.12, formula 1.13, formula 1.14, formula 1.15, formula 1.16, the said method comprising the following steps: a) The molecules denoted by Formula II and Formula III, Formula II
- Formula IV b) comprising the steps of reacting Formula IV in the presence of aryl piperazine or benzyl piperazine derivative, solvent 1, and K2CO3 to obtain Formula I or preferably Formula 1.1-Formula 1.95, particularly preferably Formula 1.1-Formula 1.16, wherein
- Y -H, -OCH3, Cl or X and Y can be -O-CH2-O- forming a combined ring and selected from
- the invention relates to a method (Method 2) for the synthesis of molecules shown in formula I, preferably molecules shown in formula 1.17 to formula 1.95, the said method comprising the following steps: 1.
- Method 2 for the synthesis of molecules shown in formula I, preferably molecules shown in formula 1.17 to formula 1.95, the said method comprising the following steps: 1.
- Formula I preferably Formula 1.1-Formula 1.95, particularly preferably Formula I.17-formula 1.95, wherein;
- Y -H, -Cl, OCH 3 or X and Y can be -O-CH2-O- forming a combined ring and selected from
- R -H, 2-CH 3 , 3-CH 3 , 4-CH 3 , 2-OCH 3 , 3-OCH 3 , 4-OCH 3 , 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO2, 3-NO2, 4-NO2, and n: 0 or 1.
- Solvent 1 and solvent 2 of Method 1 and Method 2 may be independently selected from the group consisting of DMF, DMSO, ethanol, and methanol.
- the benzyl halide or substituted benzyl halide agents in the above method can be benzyl chloride or benzyl bromide or benzyl iodide, or substituted benzyl chloride, substituted benzyl bromide, or substituted benzyl iodide.
- the invention relates, in a further aspect, to a molecule of Formula V which is used as an intermediate to obtain the molecules of Formula I and/or Formula 1.1-Formula 1.95 according to the invention.
- Y -H, Cl, OCH 3 or X and Y can be -O-CH2-O- forming a combined ring and selected from
- R -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO 2 , 3-NO2, 4-NO2.
- a preferred embodiment of the invention relates to the molecule of Formula V.1 used as an intermediate in obtaining the molecules of Formula I and/or Formula 1.1 -Formula 1.95 according to the invention.
- Formula V.l A preferred embodiment of the invention relates to the molecule of Formula V.2 used as an intermediate in obtaining the molecules of Formula I and/or Formula 1.1 -Formula 1.95 according to the invention.
- the invention relates to molecules of formula I and/or molecules of formula 1.1- Formula 1.95 according to the invention for use in the treatment of Alzheimer's disease.
- the invention relates to molecules of Formula I and/or Formula 1.1 -Formula 1.95 for use as acetylcholinesterase inhibitors.
- Example 6 Results of Molecular Modeling Studies of Formula 1.19 and Formula 1.20
- protein data bank data coded 4EY7 was used. This file was opened and processed in the Maestr Schrodinger program, its active region was defined, and the donepezil was docked and validated with a rmsd value of 0.45. It has been observed that there are similar and compatible interactions with donepezil-active site interactions described in the introduction.
- active site interactions of Formula 1.19 are examined, it is seen that the benzyl ring attached to piperazine is in aromatic interaction with Trp86.
- the carbonyl group attached to the benzofuran ring is in a hydrogen bond interaction with Phe295, similar to the carbonyl in donepezil.
- the methoxy group has an H bond interaction with the NH group of Trp286 via a double water bridge.
- the piperazine nitrogen group appears to be located in the region flanked by Tyr337, Tyr341, and Phe338, similar to piperidine in donepezil. It is also seen that the benzofuran ring has an aromatic interaction with Trp286, similar to the indenone ring in donepezil.
Abstract
The present invention relates to novel acetylcholinesterase inhibitors, methods of preparing acetylcholinesterase inhibitors according to the invention, and pharmaceutical compositions comprising the said acetylcholinesterase inhibitors.
Description
DESCRIPTION
NOVEL ACETYLCHOLINESTERASE INHIBITORS
Technical Field
The present invention relates to novel acetylcholinesterase inhibitors, methods of preparing acetylcholinesterase inhibitors of the invention, and pharmaceutical compositions comprising the said acetylcholinesterase inhibitors.
State of the Art
Alzheimer's disease (AD) is a neurodegenerative disorder that causes cognitive symptoms such as forgetfulness and behavioral disorders as a result of impaired neuronal function in the brain. The disease has not yet been fully resolved and therefore treatment is palliative. Theories such as 0- amyloid plaques, senile plaques, lysosomal dysfunction, and impaired cholinergic transmission explain the basis and symptoms of the disease. All these theories have been clearly shown to have a causal or consequential role in the disease. Therefore, drug development efforts in this area are mainly focused on targeting these pathways to develop effective small molecules. Physostigmine, a natural alkaloid isolated in the late 19th century, was the main drug used to treat Alzheimer's disease. Although tacrine was previously developed for respiratory diseases, it was later used to treat Alzheimer's disease. Rivastigmine, galantamine, and donepezil were subsequently approved. Today, donepezil, memantine, galantamine, and rivastigmine are the main drugs prescribed in the treatment of mild to moderate AD. All these drugs are reported favorably in terms of efficacy and side effects. Among them, donepezil shows the highest affinity at low micromolar levels but also has significant side effects such as extrapyramidal effects, low blood pressure, severe vomiting, bradycardia, and arrhythmia.
There is a need for alternative molecules with acetylcholinesterase inhibitor activity for the treatment of Alzheimer's disease, which is becoming more common with the prolongation of human life.
The Objects and Brief Description of the Invention
An object of the invention is to develop new acetylcholinesterase inhibitor molecules suitable for use in the treatment of Alzheimer's disease.
Another object of the invention is to develop new molecules that have the potential to be used in the treatment of Alzheimer's disease and preferably have an acetylcholinesterase inhibitor effect. In the studies carried out for this purpose, the inventors have determined that the new molecules shown in Formula I below exhibit high acetylcholinesterase inhibitor activity.
Formula I
Detailed Description of the Invention
The present invention relates to novel acetylcholinesterase derivatives molecules with potential for use in the treatment of Alzheimer's disease, and the inventive molecules are shown in Formula I below,
wherein; X: -H, -OCH3
Y: -H, -Cl, OCH3 or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3 R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO2, 3-NO2, 4-NO2, n: 0 or 1.
The molecules according to the invention are shown in Formula 1.1-1.95 in a preferred embodiment of the invention, and the variables X, Y, Z, T, R, and n of the said molecules are given in the table below (Table 1).
The molecules in Table 1 are provided only to illustrate the invention and the invention is not strictly limited to these examples. The chemical structure of the molecule according to the invention shown in Formula 1.83 is provided for a better understanding of the invention.
Formula 1.83
A preferred embodiment of the invention relates to pharmaceutically acceptable derivatives of molecules represented by Formula I and/or Formula 1.1 - Formula 1.95. The pharmaceutically acceptable derivatives mentioned herein may be salts, solvates, esters, hydrates of the molecules indicated by Formula I or Formula 1.1 - Formula 1.95. The phrase "pharmaceutically acceptable
derivatives" in this application refers to chemical or physical modifications to increase the solubility or facilitate the formulation or increase the bioavailability of molecules shown in Formula I and/or Formula 1.1 - Formula 1.95 that do not result in a change in the therapeutic activity of the molecule.
An embodiment of the invention relates to novel acetylcholinesterase inhibitor derivative molecules suitable for use in the treatment of Alzheimer's disease, and the molecules of the invention are denoted by Formula 1.1, Formula 1.2, Formula 1.3, Formula 1.4, Formula 1.5, Formula 1.6, Formula 1.7, Formula 1.8, Formula 1.9, Formula 1.10, Formula 1.11, Formula 1.12, Formula 1.13, Formula 1.14, Formula 1.15, Formula 1.16, Formula 1.17, Formula 1.18, Formula 1.19, Formula 1.20.
An embodiment of the invention relates to novel acetylcholinesterase inhibitor derivative molecules suitable for use in the treatment of Alzheimer's disease, and the molecules of the invention are denoted by Formula 1.1, Formula 1.2, Formula 1.3, Formula 1.4, Formula 1.5, Formula 1.7, Formula 1.8, Formula 1.9, Formula 1.10, Formula 1.11, Formula 1.12, Formula 1.13, Formula 1.14, Formula 1.15, Formula 1.19, Formula 1.20.
An embodiment of the invention relates to novel acetylcholinesterase inhibitor-derived molecules suitable for use in the treatment of Alzheimer's disease, and the molecules of the invention are denoted by Formula 1.1, Formula 1.2, Formula 1.3, Formula 1.4, Formula 1.5, Formula 1.7, Formula 1.8, Formula 1.9, Formula 1.10, Formula 1.11, Formula 1.12, Formula 1.13, Formula 1.14, Formula 1.15, Formula 1.19, Formula 1.20, Formula 1.21, Formula 1.22, Formula 1.23, Formula 1.24, Formula 1.25, Formula 1.26, Formula 1.27, Formula 1.28, Formula 1.29, Formula 1.30, Formula 1.31, Formula 1.32, Formula 1.32, Formula 1.33, Formula 1.34, Formula 1.35, Formula 1.36, Formula 1.37, Formula 1.38, Formula 1.39, Formula 1.40, Formula 1.41, Formula 1.42, Formula 1.43, Formula 1.44, Formula 1.45, Formula 1.46, Formula 1.47, Formula 1.48, Formula 1.49, Formula 1.50, Formula 1.51, Formula 1.52, Formula 1.53, Formula 1.54, Formula 1.55, Formula 1.56, Formula 1.57, Formula 1.58, Formula 1.59, Formula 1.60, Formula 1.61, Formula 1.62, Formula 1.63, Formula 1.64, Formula 1.65, Formula 1.66, Formula 1.67, Formula 1.68, Formula 1.69, Formula 1.70, Formula 1.71, Formula 1.72, Formula 1.73, Formula 1.74, Formula
1.75, Formula 1.76, Formula 1.77, Formula 1.78, Formula 1.79, Formula 1.80, Formula 1.81, Formula 1.82, Formula 1.82, Formula 1.83 Formula 1.84, Formula 1.85, Formula 1.86, Formula 1.87, Formula 1.88, Formula 1.89, Formula 1.90, Formula 1.91, Formula 1.92, Formula 1.93, Formula 1.94, Formula 1.95.
A further embodiment of the invention relates to pharmaceutical compositions comprising molecules indicated by Formula I and/or Formula 1.1 - Formula 1.95.
The pharmaceutical compositions according to the invention may be formulated in oral dosage forms. In pharmaceutical compositions according to the invention, pharmaceutically acceptable excipients may be used in addition to the molecules shown in Formula I and/or Formula 1.1 - Formula 1.95 used as drug substances.
The term excipient refers to substances that are used to formulate drug substances according to the invention and have no therapeutic efficacy. The said excipients can be selected from agents known to be used in pharmaceutical technology.
In another aspect, the invention relates to a method (Method 1) for the synthesis of the molecules indicated by formula I, preferably formula 1.1, formula 1.2, formula 1.3, formula 1.4, formula 1.5, formula 1.6, formula 1.7, formula 1.8, formula 1.9, formula 1.10, formula 1.11, formula 1.12, formula 1.13, formula 1.14, formula 1.15, formula 1.16, the said method comprising the following steps: a) The molecules denoted by Formula II and Formula III,
Formula II
Formula IV b) comprising the steps of reacting Formula IV in the presence of aryl piperazine or benzyl piperazine derivative, solvent 1, and K2CO3 to obtain Formula I or preferably Formula 1.1-Formula 1.95, particularly preferably Formula 1.1-Formula 1.16, wherein
X: -H, -OCH3
Y: -H, -OCH3, Cl or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3
R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO2, 3-NO2, 4-NO2, and n: 0 or 1.
In another aspect, the invention relates to a method (Method 2) for the synthesis of molecules shown in formula I, preferably molecules shown in formula 1.17 to formula 1.95, the said method comprising the following steps: 1. The molecules denoted by Formula II and Formula III,
Formula IV 2. obtaining Formula V by reacting Formula IV in the presence of piperazine, solvent 1, and
K2CO3; and
Formula V
3. reacting Formula V with benzyl halide or substituted benzyl halide in the presence of solvent 2 and K2CO3 to give Formula I, preferably Formula 1.1-Formula 1.95, particularly preferably Formula I.17-formula 1.95, wherein;
X: -H, -OCH3
Y: -H, -Cl, OCH3 or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3
R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO2, 3-NO2, 4-NO2, and n: 0 or 1.
Solvent 1 and solvent 2 of Method 1 and Method 2 may be independently selected from the group consisting of DMF, DMSO, ethanol, and methanol.
The benzyl halide or substituted benzyl halide agents in the above method can be benzyl chloride or benzyl bromide or benzyl iodide, or substituted benzyl chloride, substituted benzyl bromide, or substituted benzyl iodide.
The invention relates, in a further aspect, to a molecule of Formula V which is used as an intermediate to obtain the molecules of Formula I and/or Formula 1.1-Formula 1.95 according to the invention.
wherein;
X: -H, -OCH3
Y: -H, Cl, OCH3 or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3
R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO2, 3-NO2, 4-NO2.
A preferred embodiment of the invention relates to the molecule of Formula V.1 used as an intermediate in obtaining the molecules of Formula I and/or Formula 1.1 -Formula 1.95 according to the invention.
Formula V.l
A preferred embodiment of the invention relates to the molecule of Formula V.2 used as an intermediate in obtaining the molecules of Formula I and/or Formula 1.1 -Formula 1.95 according to the invention.
In another aspect, the invention relates to molecules of formula I and/or molecules of formula 1.1- Formula 1.95 according to the invention for use in the treatment of Alzheimer's disease.
In another aspect, the invention relates to molecules of Formula I and/or Formula 1.1 -Formula 1.95 for use as acetylcholinesterase inhibitors.
All of the inventive features contained herein may be combined if necessary. The invention will now be illustrated by way of example only for the purpose of a better understanding of the invention, without limiting the scope of the invention to the examples given herein.
EXAMPLES:
Example 1: General synthesis method of molecules indicated by Formula IV
2-hydroxy benzaldehyde derivatives (Formula II, 1 equivalence) were reacted with 4-fluoro phenacyl bromide (Formula III) and potassium carbonate (1 equivalence) in a microwave synthesizer at 850W and for 1 minute without solvent. The crude product was washed with water and then recrystallized from ethanol. This is a microwave application of the Rap-Stoermer reaction.
Benzofuran-2-yl(4-fluorophenyl)methanone
Yield: 85% e.n.: 125-127°C. Lit: 133-135°C [26, 27], FT-IR vmax (cm 1): 3127.03 (C-H), 1648.79 (C=O)
(6-Methoxybenzofuran-2-yl)(4-fluorophenyl)methanone
Yield: 80% e.n.: 163-166°C (ethanol). Lit: 156-158°C (ethyl acetate)[28], FT-IR vmax (cm 1):
3116.81-2839.36 (C-H), 1617.94 (C=O)
(5-Chlorobenzofuran-2-yl)(4-fluorophenyl)methanone
Yield: 78% e.n.: 159 - 162°C. FT-IR vmax (cm 1): 3115.32 (C-H), 1642.07 (C=O)
Example 2: General synthesis method of Formula v.l and Formula V.2 molecules
4-fluorophenyl benzofuran methanone derivatives (1 equivalence) were reacted with piperazine (2 equivalence) and K2CO3 (1 equivalence) in N, N-DMF under reflux for 8 hours until the reaction was complete. The mixture was cooled, water was added, and the resulting precipitate was filtered. It was washed with water, dried, and recrystallized from ethanol.
Benzofuran-2-yl(4-piperazine-l-yl)phenyl)methanone (Formula V.l)
Yield: 74% e.n: 263-265°C.FT-IR vmax (cm 1): 2925.18 (N-H), 2674.29-2471.31 (C-H), 1600.87 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.23 (4H, brs, pip-CH2), 3.65 (4H, brs, pip-CH2), 7.14 (2H, d, J: 8.90 Hz, Ar), 7.39 (1H, t, J: 7.66 Hz, Ar), 7.55 (1H, t, J: 7.66 Hz, Ar), 7.71-7.80 (2H, m, Ar), 7.85 (1H, d, J: 8.07 Hz, Ar), 8.01 (2H, d, J: 8.47 Hz, Ar), 9.37 (1H, s, NH). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 42.70 (piperazine CH2), 44.25 (piperazine CH2), 112.65, 114.37, 115.58, 115.63, 124.00, 124.47, 125.41, 127.06, 127.34, 128.55, 131.95, 152.65, 153.61, 155.44, 181.64 (C=O). MS (M+H): For C19H18N2O2 calculated: 307, found: 307.
(6-methoxybenzofuran-2-yl)(4-(piperazine-l-yl)phenyl)methanone (Formula V.2)
Yield: 68% e.n.: 109-114°C. FT-IR vmax (cm 1): 3263.86 (N-H), 2934.18-2753.27 (C-H), 1610.05 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 2.85 (1H, s, NH), 3.30 (4H, brs, pip- CH2), 3.62 (4H, brs, pip-CH2), 6.97-7.05 (3H, m, Ar), 7.33 (1H, d, J: 1.74 Hz, Ar), 7.62 (1H, d, J: 0.67 Hz, Ar), 7.69 (1H, d, J: 8.69 Hz, Ar), 7.94 (1H, d, J: 8.95 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 45.62 (piperazine CH2), 47.83 (piperazine CH2), 56.26 (O-CH3), 96.23, 113.50, 114.49, 115.82, 115.86, 120.54, 124.24, 126.04, 131.77, 152.40, 154.65, 157.01, 160.87, 180.88 (C=O). HRMS (M+H): For C2oH2oN203 calculated: 337.1547, found: 337.1551.
Example 3; Formula 1.1 - General synthesis method of Formula 1.16
4-fluorophenyl benzofuran methanone (1 equivalence) was reacted with 50 mmol suitable arylpiperazine derivative (1 equivalence) and K2CO3 (1 equivalence) in DMSO for 8 hours until
the reaction was complete (under reflux). The mixture was cooled, water was added, and the precipitate was filtered. It was washed with water, dried, and recrystallized from the appropriate solvent given in the characterization.
Benzofuran-2-yl(4-(4-phenylpiperazine-l-yl)phenyl)methanone (Formula LI)
Yield: 70%, e.n.: 165-167°C (EtOH). FT-IR vmax (cm 1): 3054.08-2825.09 (C-H), 1628.45 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.30 (4H, brs, piperazine), 3.57 (4H, t, J: 4.5 Hz, piperazine), 6.82 (1H, t, J: 7.25 Hz, Ar), 7.00 (2H, d, J: 7.97 Hz, Ar), 7.13 (2H, d, J: 8.81 Hz, Ar), 7.25 (2H, dd, J: 8.98 Hz, j: 7.37 Hz, Ar), 7.38 (1H, t, J: 7.20 Hz, Ar), 7.54 (1H, td, J: 7.88 Hz, j: 1.31 Hz, Ar), 7.72 (1H, s, benzofuran), 7.76 (1H, d, J: 8.90 Hz, Ar), 7.85 (1H, d, J: 7.70 Hz, Ar), 8.01 (2H, d, J: 8.99 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.87 (piperazine CH2), 48.43 (piperazine CH2), 112.61, 113.74, 115.24, 116.09, 119.67, 123.92, 124.41, 126.08, 127.37, 128.40, 129.48, 132.02, 151.20, 152.86, 154.33, 155.40, 181.42 (C=O). HRMS (M+H): For C25H22N2O2 calculated: 383.1754, found: 383.1741.
Benzofuran-2-yl(4-(4-(o-tolyl)piperazine-l-yl)phenyl)methanone (Formula 1.2)
Yield: 65%, e.n.: 131-133°C (DMSO). FT-IR vmax (cm 1): 3124.31-2827.27 (C-H), 1629.44 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 2.31 (3H, s, CH3), 2.99 (4H, brs, piperazine), 3.54 (4H, brs, piperazine), 6.96-7.21 (6H, m, Ar), 7.35-7.42 (1H, m, Ar), 7.54 (1H, tq, J: 7.68 Hz, j: 1.31 Hz, Ar), 7.71 (2H, m, Ar), 7.84 (1H, d, J: 6.94 Hz, Ar), 8.01 (2H, dd, J: 8.96 Hz,j: 2.57 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 18.08 (CH3), 47.52 (piperazine CH2), 51.62 (piperazine CH2), 112.62, 113.74, 115.25, 119.31, 123.64, 123.92, 124.41, 126.09, 127.09, 127.37, 128.39, 131.36, 132.02, 132.33, 151.38, 152.87, 154.56, 155.40, 181.40 (C=O). HRMS (M+H): For C26H24N2O2 calculated: 397.1911, found: 397.1904.
Benzofuran-2-yl(4-(4-(m-tolyl)piperazine-l-yl)phenyl)methanone (Formula 1.3)
Yield: 66%, e.n.: 146-149°C (DMSO). FT-IR vmax (cm 1): 3105.75-2834.64 (C-H), 1625.69 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 2.27 (3H, s, CH3), 3.28 (4H, t, J: 4.83 Hz,
piperazine), 3.55 (4H, t, J: 5.05 Hz, piperazine), 6.64 (1H, d, J: 7.36 Hz, Ar), 6.77-6.83 (2H, m, Ar), 7.10-7.16 (3H, m, Ar), 7.38 (1H, t, J: 7.53 Hz, Ar), 7.54 (1H, td, J: 7.72 Hz,j: 1.26 Hz, Ar), 7.71 (1H, s, benzofuran), 7.75 (1H, d, J: 8.43 Hz, Ar), 7.85 (1H, d, J: 7.60 Hz, Ar), 8.01 (2H, d, J: 8.99 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 21.89 (CH3), 46.88 (piperazine CH2), 48.55 (piperazine CH2), 112.61, 113.36, 113.74, 115.23, 115.26, 116.79, 120.57, 123.92, 124.41, 126.08, 127.37, 128.40, 129.31, 132.02, 138.57, 151.20, 152.86, 154.34, 155.40, 181.41 (C=O). HRMS (M+H): For C26H24N2O2, calculated: 397.1911, found: 397.1906.
Benzofuran-2-yl(4-(4-(p-tolyl)piperazine-l-yl)phenyl)methanone (Formula 1.4)
Yield: 53%, e.n.: 194-196°C (DMSO). FT-IR Umax (cm 1): 3059-2827.83 (C-H), 1626.90 (C=O). 1H NMR (300 MHz) DMSO-d6) 6 (ppm): 2.22 (3H, s, CH3), 3.24 (4H, t, J: 4.83 Hz, piperazine), 3.56 (4H, t, J: 5.05 Hz, piperazine), 6.91 (2H, d, J: 8.73 Hz, Ar), 7.06 (2H, d, J: 8.50 Hz, Ar), 7.14 (2H, d, J: 9.07 Hz, Ar), 7.39 (1H, t, J: 7.17 Hz, Ar), 7.55 (1H, td, J: 7.77 Hz, 1.19 Hz, Ar), 7.72 (1H, s, Ar), 7.77 (1H, d, J: 8.48 Hz, Ar), 7.85 (1H, d, J: 7.68 Hz, Ar), 8.01 (2H, d, J: 8.96 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 20.55 (CH3), 46.94 (piperazine CH2), 49.02 (piperazine CH2), 112.62, 113.78, 115.27, 116.46, 123.92, 124.42, 126.07, 127.37, 128.40, 128.60, 129.92, 132.02, 149.17, 152.85, 154.37, 155.40, 181.42 (C=O). HRMS (M+H): C26H24N2O2 calculated: 397.1911, found: 397.1916.
Benzofuran-2-yl(4-(4-(2-methoxyphenyl)piperazine-l-yl)phenyl)methanone (Formula 1.5)
Yield: 78%, e.n.: 100-101°C (DMSO). FT-IR vmax (cm 1): 3109.64-2766.46 (C-H), 1629.09 (C=O). 1H NMR (300 MHz) DMSO-d6) δ (ppm): 3.17 (4H, t, J: 4.63 Hz, piperazine), 3.60 (4H, t, J: 4.66 Hz, piperazine-CH2), 3.87 (3H, s, CH3), 6.91-7.07 (4H, m, Ar), 7.19 (2H, d, J: 9.02 Hz, Ar), 7.44 (1H, d, J: 7.51 Hz, Ar), 7.60 (2H, td, J: 7.72 Hz, 1.05 Hz, Ar), 7.78 (1H, s, Ar), 7.82 (1H, d, J: 8.35 Hz, Ar), 8.06 (2H, d, J: 8.94 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 47.26 (piperazine-CH2), 50.33 (piperazine- CH2), 55.87 (OCH3), 112.38, 112.62, 113.69, 115.21, 118.56, 121.33, 123.29, 123.92, 124.41, 126.00, 127.38, 128.39, 132.02, 141.26, 152.48, 152.87, 154.53, 155.39, 181.39 (C=O). HRMS (M+H): For C26H24N2O3 calculated: 413.1860, found: 413.1839.
Benzofuran-2-yl(4-(4-(3-methoxyphenyl)piperazine-l-yl)phenyl)methanone (Formula 1.6)
Yield: 81%, e.n.: 139-141°C (EtOH). FT-IR vmax (cm 1): 3114.97-2834.27 (C-H), 1635.89 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.31 (4H, m, piperazine), 3.56 (4H, t, J: 5.17 Hz, piperazine-CH2), 3.74 (3H, s, CH3), 6.41 (1H, dd, J: 8.02 Hz, 1.98 Hz, Ar), 6.52 (1H, t, J: 2.29 Hz, Ar), 6.59 (1H, dd, J: 8.13 Hz, 1.82 Hz, Ar), 7.11-7.18 (3H, m, Ar), 7.39 (1H, dd, J: 7.55 Hz, 0.88 Hz, Ar), 7.55 (1H, td, J: 7.58 Hz, 1.30 Hz, Ar), 7.72 (2H, d, J: 0.82 Hz, Ar), 7.74 (1H, d, J: 8.40 Hz, 0.82 Hz, Ar), 7.85 (1H, d, J: 7.57 Hz, Ar), 8.01 (2H, d, J: 8.95 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.82 (piperazine-CH2), 48.37(piperazine-CH2), 55.39 (OCH3), 102.15, 105.01, 108.65, 112.62, 113.73, 115.25, 123.92, 124.42, 126.06, 127.37, 128.41, 130.19, 132.03, 152.56, 152.85, 154.31, 155.40, 160.71, 181.42 (C=O). HRMS (M+H): For C26H24N2O3 calculated: 413.1860, found: 413.1841.
Benzofuran-2-yl(4-(4-(4-methoxyphenyl)piperazine-l-yl)phenyl)methanone (Formula 1.7) Yield: 74%, e.n.: 184.5-186°C (DMSO). FT-IR vmax (cm 1): 3114.72-2768.64 (C-H), 1621.43 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.17 (4H, t, J: 4.94 Hz, piperazine), 3.57 (4H, t, J: 4.94 Hz, piperazine- CH2), 3.70 (3H, s, CH3), 6.85 (2H, d, J: 9.10 Hz, Ar), 6.97 (2H, d, J: 9.10 Hz, Ar), 7,14 (2H, d, J: 9.10 Hz, Ar), 7.39 (1H, td, J: 7.53 Hz, 0.87 Hz, Ar), 7.55 (1H, td, J: 7.79 Hz, 1.30 Hz, Ar), 7.72 (1H, d, J: 1.03 Hz, Ar), 7.76 (1H, dd, J: 8.41 Hz, 0.77 Hz, Ar), 7.85 (2H, d, J: 7.61 Hz, Ar), 8.01 (2H, d, J: 9.00 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 47.06 (piperazine-CH2), 50.02 (piperazine- CH2), 55.67 (OCH3), 112.62, 113.80, 114.79, 115.26, 118.29, 123.93, 124.42, 126.07, 127.37, 128.41, 132.02, 145.59, 152.84, 153.73, 154.41, 155.39, 181.42 (C=O). HRMS (M+H): For C26H24N2O3 calculated: 413.1860, found: 413.1840.
Benzofuran-2-yl(4-(4-(2-chlorophenyl)piperazine-l-yl)phenyl)methanone (Formula 1.8)
Yield: 66%, e.n.: 153.4-156°C (DMSO)F.T-IR vmax (cm 1): 3075.73-2764.49 (C-H), 1625.19 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.14 (4H, t, J: 4.75 Hz, piperazine), 3.57 (4H, t, J: 4.91 Hz, piperazine-CH2), 7.08 (1H, td, J: 7.58 Hz, 1.57 Hz, Ar), 7.14 (2H, d, J: 9.18 Hz, Ar), 7.21 (1H, dd, J: 8.12 Hz, 1.55 Hz, Ar), 7.30-7.39 (2H, m, Ar), 7.44 (1H, td, J: 8.37 Hz, 1.52
Hz, Ar), 7.54 (1H, td, J: 7.8 Hz, 1.28 Hz, Ar), 7.73 (1H, d, J: 0.92 Hz, Ar), 7.74 (1H, dd, J: 8.40 Hz, 0.80 Hz, Ar), 7.85 (1H, d, J: 7.63 Hz, Ar), 8.01 (2H, d, J: 9.01 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 5 (ppm): 47.27 (piperazine-CH2), 51.07 (piperazine-CH2), 112.62, 113.80, 115.28, 121.44, 123.93, 124.41, 124.70, 126.17, 127.37, 128.13, 128.41, 128.64, 130.86, 132.01, 149.11, 152.83, 154.47, 155.40, 181.43 (C=O). HRMS (M+H): For C25H21C1N2O2 calculated: 417.1364, found: 417.1376.
Benzofuran-2-yl(4-(4-(3-chlorophenyl)piperazine-l-yl)phenyl)methanone (Formula L9)
Yield: 80%, e.n.: 167-170°C (DMSO). FT-IR vmax (cm 1): 3144.85-2819.94 (C-H), 1602.76 (C=O). NMR (300 MHz) DMSO-d6 5 (ppm): 3.37 (4H, m, piperazine), 3.56 (4H, t, J: 4.97 Hz, piperazine-CH2), 6.82 (1H, td, J: 7.88 Hz, 1.35 Hz, Ar), 6.96 (1H, dd, J: 8.38 Hz, 1.85 Hz, Ar), 7.01 (1H, t, J: 2.04 Hz, Ar), 7.12 (1H, d, J: 9.12 Hz, Ar), 7.25 (1H, t, J: 8.15 Hz, Ar), 7.38 (1H, td, J: 7.48 Hz, 0.85 Hz, Ar), 7.54 (1H, td, J: 7.81 Hz, 1.28 Hz, Ar), 7.72 (1H, d, J: 0.86 Hz, Ar), 7.76 (1H, dd, J: 8.40 Hz, 0.74 Hz, Ar), 7.85 (1H, d, J: 7.65 Hz, Ar), 8.01 (2H, d, J: 8.98 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 5 (ppm): 46.62 (piperazine-CH2), 47.71 (piperazine-CH2), 112.62, 113.71, 114.22, 115.12, 115.26, 118.77, 123.92, 124.42, 126.09, 127.36, 128.41, 130.97, 132.03, 134.34, 152.37, 152.84, 154.21, 155.40, 181.42 (C=O). HRMS (M+H): For C25H2IC1N2O2 calculated: 417.1364 found: 417.1353.
Benzofuran-2-yl(4-(4-(4-chlorophenyl)piperazine-l-yl)phenyl)methanone (Formula LIO)
Yield: 76%, e.n.: 200-202°C (DMSO). FT-IR vmax (cm -1): 3069.82-2837.21 (C-H), 1623.42 (C=O). 1H NMR (300 MHz) DMSO-d6 5 (ppm): 3.29 (4H, m, piperazine CH2), 3.57 (4H, t, J: 5.07 Hz, piperazine CH2), 7.02 (2H, d, J: 9.11 Hz, Ar), 7.13 (2H, d, J: 9.11 Hz, Ar), 7.27 (2H, d, J: 7.28 Hz, Ar), 7.39 (1H, td, J: 8.00 Hz, 0.77 Hz, Ar), 7.55 (1H, td, J: 7.81 Hz, 1.22 Hz, Ar), 7.72 (1H, d, J: 0.88 Hz, Ar), 7.76 (1H, dd, J: 8.37 Hz, 0.68 Hz, Ar), 7.85 (1H, d, J: 7.72 Hz, Ar), 8.01 (2H, d, J: 9.04 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 5 (ppm): 46.71 (piperazine-CH2), 48.17 (piperazine-CH2), 112.62, 113.76, 115.29, 117.49, 123.12, 123.93, 124.42, 126.10, 127.36, 128.41, 129.17, 132.02, 149.99, 152.83, 154.25, 155.39, 181.43 (C=O). HRMS (M+H): For C25H2IC1N2O2 calculated: 417.1364 found: 417.1380.
(6-Methoxybenzofuran-2-yl) (4-(4-phenylpiperazine-l-yl)phenyl)methanone (Formula Lil)
Yield: 77%, e.n.: 184.5-186°C (DMSO). FT-IR vmax (cm 1): 3124.61-2830.25 (C-H), 1590.05 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.30 (4H, m, piperazine CH2), 3.55 (4H, t, J: 4.99 Hz, piperazine CH2), 3.86 (3H, s, CH3), 6.82 (1H, d, J: 7.23 Hz, Ar), 7.01 (3H, dd, J: 8.69 Hz, 2.08 Hz, Ar), 7.12 (2H, d, J: 9.05 Hz, Ar), 7.25 (2H, t, J: 7.97 Hz, Ar), 7.35 (1H, d, J: 1.57 Hz, Ar), 7.66 (1H, d, J: 0.79 Hz, Ar), 7.71 (1H, d, J: 8.67 Hz, Ar), 7.98 (2H, d, J: 8.92 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.95 (piperazine-CH2), 48.46 (piperazine-CH2), 56.24 (OCH3), 96.26, 113.80, 114.54, 115.99, 116.10, 119.68, 120.55, 124.29, 126.43, 129.49, 131.80, 151.21, 152.36, 154.19, 157.05, 160.92, 180.98 (C=O). HRMS (M+H): For C26H24N2O3 calculated: 413.1860, found: 413.1843.
(6-Methoxybenzofuran-2-yl) (4-(4-(4-methoxyphenyl)piperazine-l-yl)phenyl)methanone (Formula 1.12)
Yield: 68%, e.n.: 202-204°C (DMSO). FT-IR vmax (cm 1): 2959.30-2831.36 (C-H), 1614.84 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.17 (4H, brs, piperazine CH2), 3.54 (4H, brs, piperazine CH2), 3.70 (3H, s, CH3), 3.87 (3H, s, CH3), 6.86 (2H, d, J: 9.26 Hz, Ar), 6.92-7.06 (3H, m, Ar), 7.13 (2H, d, J: 8.63 Hz, Ar), 7.35 (1H, s, Ar), 7.66 (1H, s, Ar), 7.71 (1H, d, J: 8.63 Hz, Ar), 7.97 (2H, d, J: 8.88 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 47.14 (piperazine- CH2), 50.03 (piperazine-CH2), 55.75 (OCH3), 96.25, 113.85, 114.54, 114.78, 118.28, 124.29, 126.42, 131.78, 145.60, 154.26, 160.92, 180.97 (C=O). HRMS (M+H): C27H26N2O4 calculated: 443.1965, found: 443.1971.
(4-(4-(4-Chlorophenyl)piperazine-l-yl)phenyl) (6-methoxybenzofuran-2-yl)methanone (Formula 1.13)
Yield: 80%, e.n.: 205-207°C (DMSO). FT-IR vmax (cm 1): 3120.72-2830.72 (C-H), 1613.78 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.30 (4H, m, piperazine CH2), 3.55 (4H, t, J: 5.08 Hz, piperazine CH2), 3.86 (3H, s, OCH3), 6.96-7.06 (3H, m, Ar), 7.12 (2H, d, J: 9.04 Hz,
Ar), 7.27 (2H, d, J: 9.04 Hz, Ar), 7.35 (1H, d, J: 1.57 Hz, Ar), 7.66 (1H, d, J: 0.83 Hz, Ar), 7.71 (1H, d, J: 8.64 Hz, Ar), 7.97 (2H, d, J: 8.89 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.80 (piperazine-CH2), 48.19 (piperazine-CH2), 58.85 (OCH3), 96.26, 113.82, 114.55, 117.51, 124.29, 129.17, 131.80, 147.83, 150.11, 154.11, 160.98, 182.81 (C=O). HRMS (M+H): For C26H23C1N2O3 calculated: 447.1470, found: 447.1488.
(5-Chlorobenzofuran-2-yl) (4-(4-phenylpiperazine-l-yl)phenyl)methanone (Formula 1.14)
Yield: 65%, e.n.: 201.5-203°C (DMSOF).T-IR vmax (cm 1): 3109.92-2839 (C-H), 1626.47 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.57 (4H, m, piperazine CH2), 3.74 (4H, m, piperazine CH2), 6.82 (1H, t, J: 7.18 Hz, Ar), 7.01 (1H, d, J: 7.20 Hz, Ar), 7.14 (2H, dd, J: 9.10 Hz, 1.75 Hz, Ar), 7.25 (2H, td, J: 8.07 Hz, 1.93 Hz, Ar), 7.57 (1H, td, J: 8.83 Hz, 2.12 Hz, Ar), 7.68 (1H, m, Ar), 7.81 (1H, d, J: 9.37 Hz, Ar), 7.92 (1H, t, J: 1.95 Hz, Ar), 8.00 (2H, dd, J: 8.99 Hz, 1.86 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.8 (piperazine-CH2), 48.42 (piperazine- CH2), 113.71, 114.41, 114.52, 116.09, 119.68, 123.14, 125.71, 128.29, 128.68, 128.94, 129.49, 132.11, 151.18, 154.44, 180.97 (C=O). HRMS (M+H): For C25H2iCl2N2O2 calculated: 417.1364, found: 417.1359.
(5-Chlorobenzofuran-2-yl) (4-(4-(4-methoxyphenyl)piperazine-l-yl)phenyl)methanone (Formula 1.15)
Yield: 80%, e.n.: 198-200°C (DMSO). FT-IR vmax (cm 1): 3000-2835.73 (C-H), 1626.21 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.16 (4H, brs, piperazine CH2), 3.57 (4H, brs, piperazine CH2), 3.70 (3H, s, OCH3), 6.85 (2H, d, J: 8.67 Hz, Ar), 6.97 (2H, d, J: 8.67 Hz, Ar), 7.14 (2H, d, J: 8.86 Hz, Ar), 7.57 (1H, d, J: 8.65 Hz, Ar), 7.68 (1H, s, Ar), 7.81 (1H, d, J: 8.88 Hz, Ar), 7.93 (1H, s, Ar), 8.00 (2H, d, J: 8.65 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 47.00 (piperazine-CH2), 50.01 (piperazine-CH2), 113.77, 114.77, 118.29, 121.81, 122.38, 123.23, 125.92, 128.30, 132.11, 134.13, 136.54, 138.67, 145.75, 154.67, 181.30 (C=O). HRMS (M+H): For C26H23C1N2O3 calculated: 447.1470, found: 447.1471.
(5-Chlorobenzofuran-2-yl) (4-(4-(4-chlorophenyl)piperazine-l-yl)phenyl)methanone
(Formula 1.16)
Yield: 74%, e.n.: 210-212°C (DMSO). FT-IR vmax (cm 1): 3107.79-2826.69 (C-H), 1626.39 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.30 (4H, m, piperazine CH2), 3.58 (4H, brs, piperazine CH2), 7.02 (2H, d, J: 9.08 Hz, Ar), 7.14 (2H, d, J: 8.48 Hz, Ar), 7.27 (2H, d, J: 8.96 Hz, Ar), 7.56 (1H, d, J: 8.72 Hz, 2.18 Hz, Ar), 7.67 (1H, s, Ar), 7.81 (1H, d, J: 8.72 Hz, Ar), 7.93 (1H, d, J: 2.08 Hz, Ar), 8.00 (2H, d, J: 8.92 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.65 (piperazine-CH2), 48.15 (piperazine-CH2), 88.05, 113.72, 114.40, 114.55, 117.48, 123.12, 128.29, 129.17, 132.11, 149.11, 152.21, 153.98, 167.70, 184.51 (C=O). HRMS (M+H): For C25H20Cl2N2O2 calculated: 451.0975, found: 451.0981.
Example 4: General Synthesis Method of Formula L17-Formula 1.95
The molecules indicated by formula V (1 equivalence) are reacted in DMSO using benzyl chloride or 4-chlorobenzyl (1 equivalence) under reflux for 6 hours in the presence of FGCO-, (1 equivalence). After the reaction is completed, water is added, and the resulting precipitate is collected. The collected precipitate is washed twice with water and recrystallized from ethanol.
Benzofuran-2-yl(4-(4-benzylpiperazine-l-yl)phenyl)methanone (Formula 1.17)
Yield: 68% e.n.: 164-167°C. FT-IR vmax (cm 1): 3124.17 - 2776.43 (C-H), 1591.07 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 2.52 (4H, m, piperazine CH2), 3.40 (4H, t, J: 4.60 Hz, piperazine CH2), 3.53 ;(2H, s, CH2), 7.23-7.44 (6H, m, Ar), 7.53 (1H, d, J: 7.81 Hz, Ar), 7.71 (1H, s, Ar), 7.75 (1H, d, J: 8.44 Hz, Ar), 7.84 (1H, d, J: 7.81 Hz, Ar), 7.97 (2H, d, J: 8.86 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.95 (piperazine-CH2), 52.69 (piperazine-CH2), 62.41 (benzyl-CH2), 112.61, 113.60, 115.20, 115.24, 123.91, 124.40, 125.84, 127.36, 127.51, 128.38, 128.70, 129.41, 131.99, 138.36, 152.84, 154.47, 155.37, 181.36 (C=O). HRMS (M+H): For C26H24N2O2 calculated: 397.1911, found: 397.1921.
Benzofuran-2-yl(4-(4-chlorobenzyl)piperazine- l-yl)phenyl)methanone (F ormula 1.18)
Yield: 70% e.n.: 177-179°C. FT-IR vmax (cm 1): 3104.42-2828.01 (C-H), 1602.01 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 3.30-3.71 (10H, m, piperazine CH2, benzyl CH2), 6.89- 8.18 (12H, m, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 46.94 (piperazine-CH2), 52.62 (piperazine- CH2), 61.42 (benzyl-CH2), 112.62, 113.63, 115.22, 123.92, 124.41, 125.87, 127.36, 128.39, 128.68, 131.17, 132.00, 137.50, 152.82, 154.45, 155.37, 181.37 (C=O). HRMS (M+H): For C26H23C1N2O2 calculated: 431.1521, found: 431.1541.
(4-(4-benzylpiperazine-l-yl)phenyl) (6-methoxybenzofuran-2-yl)methanone (Formula 1.19);
Yield: 70% e.n.: 158-161°C. FT-IR vmax (cm 1): 3107.96-2773.77 (C-H), 1602.95 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 2.52 (4H, m, piperazine CH2), 3.38 (4H, t, J: 4.60 Hz, piperazine CH2), 3.53 ;(2H, s, CH2), 3.85 (3H, s, Ar), 6.94-7.10 (3H, m, Ar), 7.22-7.41 (6H, m, Ar), 7.64 (1H, s, Ar), 7.70 (1H, d, J: 8.37 Hz, Ar), 7.94 (2H, d, J: 8.86 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 47.01 (piperazine-CH2), 52.70 (piperazine-CH2), 56.26 (OCH3), 62.42 (benzyl-CH2), 96.24, 113.64, 114.52, 115.92, 120.54, 124.26, 126.19, 127.50, 128.70, 129.41, 131.76, 138.37, 152.37, 154.32, 157.02, 160.89, 180.92 (C=O). HRMS (M+H): For C27H26N2O3 calculated: 427.2016, found: 427.2016.
(4-(4-(4-chlorobenzyl)piperazine-l-yl)phenyl) (6-methoxybenzofuran-2-yl)methanone (Formula 1.20)
Yield: 75% e.n.: 218-220°C. FT-IR vmax (cm 1): 3108.20-2835.19 (C-H), 1602.28 (C=O). 1H NMR (300 MHz) DMSO-d6 δ (ppm): 2.54 (4H, m, piperazine CH2), 3.341 (4H, m, piperazine CH2), 3.53 ;(2H, s, CH2), 3.86 (3H, s, Ar), 6.97-7.09 (3H, m, Ar), 7.32-7.47 (5H, m, Ar), 7.65 (1H, s, Ar), 7.70 (1H, d, J: 8.65 Hz, Ar), 7.94 (2H, d, J: 9.14 Hz, Ar). 13C NMR (75 MHz) DMSO-d6 δ (ppm): 52.64 (OCH3), 60.31 (piperazine CH2), 94.88, 113.67, 128.68, 131.17, 131.77, 142.13, 148.28, 151.35, 154.04, 156.35, 175.17, 187.85 (C=O). HRMS (M+H): For C27H25C1N2O3 calculated: 461.1626, found: 461.1632.
Example 5: Analysis of Acetylcholinesterase (AchE) and Butyl cholinesterase (BuChE)
Enzyme Inhibition of Formula 1.1 - Formula 1.22
When the enzyme activities of the compounds were analyzed, it was observed that they did not affect butyrylcholinesterase, while they were effective on acetylcholinesterase. The main reason for this is believed to be a binding mode similar to donepezil, which is the binding mode specific to acetylcholinesterase. According to these results, the structure-activity evaluation obtained from the compounds is quite parallel to donepezil structure-activity relationships.
Example 6: Results of Molecular Modeling Studies of Formula 1.19 and Formula 1.20
In molecular modeling studies, protein data bank data coded 4EY7 was used. This file was opened and processed in the Maestr Schrodinger program, its active region was defined, and the donepezil was docked and validated with a rmsd value of 0.45. It has been observed that there are similar and compatible interactions with donepezil-active site interactions described in the introduction. When the active site interactions of Formula 1.19 are examined, it is seen that the benzyl ring attached to piperazine is in aromatic interaction with Trp86. The carbonyl group attached to the benzofuran ring is in a hydrogen bond interaction with Phe295, similar to the carbonyl in donepezil. The methoxy group has an H bond interaction with the NH group of Trp286 via a double water bridge. The piperazine nitrogen group appears to be located in the region flanked by Tyr337, Tyr341, and Phe338, similar to piperidine in donepezil. It is also seen that the benzofuran ring has an aromatic interaction with Trp286, similar to the indenone ring in donepezil.
When the active site interactions of Formula 1.20 are examined, it is seen that the benzyl ring attached to piperazine is in aromatic interaction with Trp86. The nitrogen group of piperazine is in 7t- cation interaction with Tyr 337 and is also located in the region surrounded by Tyr337, Tyr341, and Phe338, similar to piperidine in donepezil. It is also seen that the benzofuran ring is in aromatic interaction with Trp286, similar to the indenone ring in donepezil. The methoxy group attached to the benzofuran ring appears to be positioned to hydrogen bond with the water molecules HOH793 and HOH953.
All these results demonstrate the potential of the compounds of Formula I according to the invention to be acetylcholinesterase inhibitors.
Claims
CLAIMS A molecule suitable for use in the treatment of Alzheimer's disease and characterized in that it is shown by following Formula I,
Formula I wherein,
X: -H, -OCH3
Y: -H, -OCH3, Cl or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3
R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2- NO2, 3-NO2, 4-NO2, n: 0 or 1. Pharmaceutical compositions comprising as drug substance the molecule indicated by Formula I according to Claim 1. A pharmaceutical composition according to Claim 2, characterized in that it comprises a pharmaceutically acceptable excipient. A method (Method 1) to be used in the preparation of molecules indicated by Formula I according to Claim 1, characterized in that it comprises the following:
a) The molecules denoted by Formula II and Formula III,
F
Formula III b) reacting in the presence of K2CO3 to obtain the molecule indicated by Formula IV; and
Formula IV c) comprising the steps of reacting Formula IV in the presence of aryl piperidine or benzyl piperazine derivative, solvent 1, and K2CO3 to obtain Formula I or preferably Formula I.l-Formula 1.95 or particularly preferably Formula I.l-Formula 1.16, wherein;
X: -H, -OCH3
Y: -H, -OCH3, Cl or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3
R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO2, 3-NO2, 4-NO2, and n: 0 or 1. A method (Method 2) to be used in the preparation of molecules indicated by Formula I according to Claim 1, characterized in that it comprises the following: a) The molecules denoted by Formula II and Formula III,
b) reacting in the presence of K2CO3 to obtain the molecule indicated by Formula IV; and
c) obtaining Formula V by reacting Formula IV in the presence of piperazine, solvent 1, and
Formula V d) the reaction of Formula V with benzyl halide or substituted benzyl halide in the presence of solvent 2 and K2CO3 to give Formula I, preferably Formula I.l-Formula 1.95, wherein;
X: -H, -OCH3
Y: -H, -OCH3, Cl or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3
R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-NO2, 3-NO2, 4-NO2, and n: 0 or 1. A method according to Claim 5, characterized in that the benzyl halide is selected from benzyl chloride, benzyl bromide, benzyl iodide or substituted benzyl chloride or substituted benzyl bromide or substituted benzyl iodide. A molecule of Formula V for use as an intermediate in the preparation of molecules according to Claim 1 ;
X: -H, -OCH3
Y: -H, -OCH3, Cl or X and Y can be -O-CH2-O- forming a combined ring and selected from
Z: -H, -OCH3
T: -H, -OCH3
R: -H, 2-CH3, 3-CH3, 4-CH3, 2-OCH3, 3-OCH3, 4-OCH3, 2-C1, 3-C1, 4-C1, 2-F, 3-F, 4-F, 2-
NO2, 3-NO2, 4-NO2.
8. A molecule according to Claim 7, characterized in that it is represented by Formula V.l and/or Formula V.2.
Formula V.2
9. Molecules indicated by Formula I according to Claim 1 for use as an acetylcholinesterase inhibitor.
10. Molecules indicated by Formula I according to Claim 1 for use in the treatment of Alzheimer's disease.
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