WO2023230604A3 - Methods of preparing rna samples for sequencing, sequencing rna, and preparing rna molecules with modified nucleic acids - Google Patents
Methods of preparing rna samples for sequencing, sequencing rna, and preparing rna molecules with modified nucleic acids Download PDFInfo
- Publication number
- WO2023230604A3 WO2023230604A3 PCT/US2023/067546 US2023067546W WO2023230604A3 WO 2023230604 A3 WO2023230604 A3 WO 2023230604A3 US 2023067546 W US2023067546 W US 2023067546W WO 2023230604 A3 WO2023230604 A3 WO 2023230604A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- rna
- sequencing
- preparing
- methods
- sample
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 7
- 238000012163 sequencing technique Methods 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108060004795 Methyltransferase Proteins 0.000 abstract 3
- 239000013614 RNA sample Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Disclosed herein are methods of preparing an RNA sample for sequencing. In certain embodiments, the method includes contacting an RNA molecule in the sample with an RNA- dependent RNA polymerase (RdRp) such that the RdRp extends the RNA molecule from the 3' end of the RNA molecule using the RNA molecule as a template. Also disclosed herein are kits for preparing an RNA sample for sequencing according to certain methods, as well as methods of sequencing RNA molecules using the prepared sample. Also disclosed herein are methods of preparing an RNA molecule with a modified base. In certain embodiments, the method includes ligating a left-arm RNA segment, a middle RNA segment including the modified base, and a right-arm RNA segment in the presence of a DNA splint and DNA disruptors.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263346650P | 2022-05-27 | 2022-05-27 | |
| US63/346,650 | 2022-05-27 | ||
| US202263433180P | 2022-12-16 | 2022-12-16 | |
| US63/433,180 | 2022-12-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2023230604A2 WO2023230604A2 (en) | 2023-11-30 |
| WO2023230604A3 true WO2023230604A3 (en) | 2024-02-08 |
Family
ID=88920130
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2023/067546 WO2023230604A2 (en) | 2022-05-27 | 2023-05-26 | Methods of preparing rna samples for sequencing, methods of sequencing rna, and methods of preparing rna molecules with modified mucleic acids |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2023230604A2 (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150072869A1 (en) * | 2013-09-06 | 2015-03-12 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
-
2023
- 2023-05-26 WO PCT/US2023/067546 patent/WO2023230604A2/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150072869A1 (en) * | 2013-09-06 | 2015-03-12 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
Non-Patent Citations (1)
| Title |
|---|
| MAIDA: "De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase", MOLECULAR AND CELLULAR BIOLOGY, 1 April 2016 (2016-04-01), pages 1248 - 1259, XP055543463, DOI: 10.1128/MCB.01021-15 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023230604A2 (en) | 2023-11-30 |
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