WO2023225651A3 - Pre-amplification-free target sequence detection with colormetric readout - Google Patents
Pre-amplification-free target sequence detection with colormetric readout Download PDFInfo
- Publication number
- WO2023225651A3 WO2023225651A3 PCT/US2023/067243 US2023067243W WO2023225651A3 WO 2023225651 A3 WO2023225651 A3 WO 2023225651A3 US 2023067243 W US2023067243 W US 2023067243W WO 2023225651 A3 WO2023225651 A3 WO 2023225651A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- systems
- nucleic acids
- amplification
- layer
- module
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
Abstract
Systems comprising a translation module, an amplification module comprising one or more sensing modalities, and a detection module configured to accept the amplifier output and methods of using the same are provided. The systems or methods can be capable of detecting trace amounts of nucleic acid molecules with high sensitivity and specificity in a device-free system with naked-eye readable output. The systems can comprise three layers incorporated into a lateral flow assay and/or a paper strip assay. In such systems, the first layer can comprise the translation module, the second layer can comprise the amplification module, and the third layer can comprise the detection module. These systems can be self-catalytic (auto-catalytic), and may employ cryptic nucleic acids to enhance signal. The cryptic nucleic acids may be split trigger nucleic acids and/or modified nucleic acids.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263343931P | 2022-05-19 | 2022-05-19 | |
| US63/343,931 | 2022-05-19 | ||
| US202318252869A | 2023-05-12 | 2023-05-12 | |
| US18/252,869 | 2023-05-12 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2023225651A2 WO2023225651A2 (en) | 2023-11-23 |
| WO2023225651A3 true WO2023225651A3 (en) | 2023-12-28 |
| WO2023225651A9 WO2023225651A9 (en) | 2024-04-04 |
Family
ID=88836192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2023/067243 WO2023225651A2 (en) | 2022-05-19 | 2023-05-19 | Pre-amplification-free target sequence detection with colormetric readout |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2023225651A2 (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160083785A1 (en) * | 2012-06-18 | 2016-03-24 | Speedx Pty Ltd | Target detection and signal amplification |
| US20190309357A1 (en) * | 2016-12-09 | 2019-10-10 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
-
2023
- 2023-05-19 WO PCT/US2023/067243 patent/WO2023225651A2/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160083785A1 (en) * | 2012-06-18 | 2016-03-24 | Speedx Pty Ltd | Target detection and signal amplification |
| US20190309357A1 (en) * | 2016-12-09 | 2019-10-10 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023225651A9 (en) | 2024-04-04 |
| WO2023225651A2 (en) | 2023-11-23 |
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