WO2023217767A3 - Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning - Google Patents

Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning Download PDF

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Publication number
WO2023217767A3
WO2023217767A3 PCT/EP2023/062247 EP2023062247W WO2023217767A3 WO 2023217767 A3 WO2023217767 A3 WO 2023217767A3 EP 2023062247 W EP2023062247 W EP 2023062247W WO 2023217767 A3 WO2023217767 A3 WO 2023217767A3
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WIPO (PCT)
Prior art keywords
organoid
farms
regional
patterning
aggregation
Prior art date
Application number
PCT/EP2023/062247
Other languages
French (fr)
Other versions
WO2023217767A2 (en
Inventor
Christopher Lawrence GRIGSBY
Kaja I. RITZAU-REID
Richard Wang
Ruoxiao XIE
Daniel REUMANN
Jürgen A. KNOBLICH
James P. Armstrong
Jonathon YEOW
Molly M. Stevens
Original Assignee
Imperial College Innovations Limited
Institut Für Molekulare Biotechnologie Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Imperial College Innovations Limited, Institut Für Molekulare Biotechnologie Gmbh filed Critical Imperial College Innovations Limited
Publication of WO2023217767A2 publication Critical patent/WO2023217767A2/en
Publication of WO2023217767A3 publication Critical patent/WO2023217767A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y70/00—Materials specially adapted for additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y80/00—Products made by additive manufacturing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/72—Chitin, chitosan

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Manufacturing & Machinery (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Materials Engineering (AREA)
  • Immunology (AREA)
  • Transplantation (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods and products for preparing organoids in regular arrays on scaffolds are proposed.
PCT/EP2023/062247 2022-05-09 2023-05-09 Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning WO2023217767A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2206768.0 2022-05-09
GB202206768 2022-05-09

Publications (2)

Publication Number Publication Date
WO2023217767A2 WO2023217767A2 (en) 2023-11-16
WO2023217767A3 true WO2023217767A3 (en) 2024-02-15

Family

ID=86497451

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2023/062247 WO2023217767A2 (en) 2022-05-09 2023-05-09 Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning

Country Status (1)

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WO (1) WO2023217767A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011008986A2 (en) * 2009-07-15 2011-01-20 California Institute Of Technology A method applying hemodynamic forcing and klf2 to initiate the growth and development of cardiac valves
US20170246350A1 (en) * 2010-03-04 2017-08-31 University Of Pittsburgh - Of The Commonwealth System Of Higher Education "Bioengineered Human Corneal Stromal Tissue"
US20180243480A1 (en) * 2015-08-17 2018-08-30 The Johns Hopkins University Mesenchymal cell-binding composite material for tissue restoration
US20190241849A1 (en) * 2016-08-31 2019-08-08 University Of Kansas Expandable cell culture substrate
US20190367884A1 (en) * 2017-01-08 2019-12-05 Ramot At Tel-Aviv University Ltd. Three-dimensional tumor models, methods of manufacturing same and uses thereof
US20210403850A1 (en) * 2017-01-18 2021-12-30 National Agriculture And Food Research Organization Semipermeable membrane and uses thereof
WO2022076519A1 (en) * 2020-10-08 2022-04-14 Corning Incorporated Cell culture harvest and reseed methods and systems using dissolvable substrates

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011008986A2 (en) * 2009-07-15 2011-01-20 California Institute Of Technology A method applying hemodynamic forcing and klf2 to initiate the growth and development of cardiac valves
US20170246350A1 (en) * 2010-03-04 2017-08-31 University Of Pittsburgh - Of The Commonwealth System Of Higher Education "Bioengineered Human Corneal Stromal Tissue"
US20180243480A1 (en) * 2015-08-17 2018-08-30 The Johns Hopkins University Mesenchymal cell-binding composite material for tissue restoration
US20190241849A1 (en) * 2016-08-31 2019-08-08 University Of Kansas Expandable cell culture substrate
US20190367884A1 (en) * 2017-01-08 2019-12-05 Ramot At Tel-Aviv University Ltd. Three-dimensional tumor models, methods of manufacturing same and uses thereof
US20210403850A1 (en) * 2017-01-18 2021-12-30 National Agriculture And Food Research Organization Semipermeable membrane and uses thereof
WO2022076519A1 (en) * 2020-10-08 2022-04-14 Corning Incorporated Cell culture harvest and reseed methods and systems using dissolvable substrates

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Publication number Publication date
WO2023217767A2 (en) 2023-11-16

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