WO2023200404A3 - Method for determining cfdna fragment size ratio and fragment size distribution - Google Patents

Method for determining cfdna fragment size ratio and fragment size distribution Download PDF

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Publication number
WO2023200404A3
WO2023200404A3 PCT/SG2023/050252 SG2023050252W WO2023200404A3 WO 2023200404 A3 WO2023200404 A3 WO 2023200404A3 SG 2023050252 W SG2023050252 W SG 2023050252W WO 2023200404 A3 WO2023200404 A3 WO 2023200404A3
Authority
WO
WIPO (PCT)
Prior art keywords
fragment size
determining
cfdna
size distribution
size ratio
Prior art date
Application number
PCT/SG2023/050252
Other languages
French (fr)
Other versions
WO2023200404A2 (en
Inventor
Yukti CHOUDHURY
Min-Han Tan
Original Assignee
Lucence Life Sciences Pte. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lucence Life Sciences Pte. Ltd. filed Critical Lucence Life Sciences Pte. Ltd.
Publication of WO2023200404A2 publication Critical patent/WO2023200404A2/en
Publication of WO2023200404A3 publication Critical patent/WO2023200404A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed is a method of determining cell-free DNA (cfDNA) fragment size ratio and/or fragment size distribution in a biological sample. Also disclosed is a kit for determining cfDNA fragment size ratio and/or fragment size distribution in a biological sample.
PCT/SG2023/050252 2022-04-13 2023-04-13 Method for determining cfdna fragment size ratio and fragment size distribution WO2023200404A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10202203861P 2022-04-13
SG10202203861P 2022-04-13

Publications (2)

Publication Number Publication Date
WO2023200404A2 WO2023200404A2 (en) 2023-10-19
WO2023200404A3 true WO2023200404A3 (en) 2023-11-23

Family

ID=88330447

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG2023/050252 WO2023200404A2 (en) 2022-04-13 2023-04-13 Method for determining cfdna fragment size ratio and fragment size distribution

Country Status (1)

Country Link
WO (1) WO2023200404A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060127922A1 (en) * 2001-01-05 2006-06-15 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
US20080248471A1 (en) * 1999-12-07 2008-10-09 Shuber Anthony P Methods for disease detection
US20100151478A1 (en) * 1999-09-08 2010-06-17 Genzyme Corporation Methods for disease detection
WO2020172566A1 (en) * 2019-02-22 2020-08-27 Accuragen Holdings Limited Methods and compositions for early cancer detection
US20210198747A1 (en) * 2018-05-18 2021-07-01 The Johns Hopkins University Cell-free dna for assessing and/or treating cancer

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100151478A1 (en) * 1999-09-08 2010-06-17 Genzyme Corporation Methods for disease detection
US20080248471A1 (en) * 1999-12-07 2008-10-09 Shuber Anthony P Methods for disease detection
US20060127922A1 (en) * 2001-01-05 2006-06-15 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
US20210198747A1 (en) * 2018-05-18 2021-07-01 The Johns Hopkins University Cell-free dna for assessing and/or treating cancer
WO2020172566A1 (en) * 2019-02-22 2020-08-27 Accuragen Holdings Limited Methods and compositions for early cancer detection

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHOUDHURY YUKTI, PINWEHA PANNAPA, POH JONATHAN, LIM JING SHAN, TAN MIN-HAN: "Early detection of cancer using cell-free DNA (cfDNA) size analysis on a multiplexed amplicon-based next-generation sequencing (NGS) platform.", JOURNAL OF CLINICAL ONCOLOGY, AMERICAN SOCIETY OF CLINICAL ONCOLOGY, US, vol. 40, no. 16_suppl, 1 June 2022 (2022-06-01), US , pages 3041 - 3041, XP093113647, ISSN: 0732-183X, DOI: 10.1200/JCO.2022.40.16_suppl.3041 *
UDOMRUK SASIMOL; ORRAPIN SANTHASIRI; PRUKSAKORN DUMNOENSUN; CHAIYAWAT PARUNYA: "Size distribution of cell-free DNA in oncology", CRITICAL REVIEWS IN ONCOLOGY/HEMATOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 166, 28 August 2021 (2021-08-28), AMSTERDAM, NL , XP086812493, ISSN: 1040-8428, DOI: 10.1016/j.critrevonc.2021.103455 *

Also Published As

Publication number Publication date
WO2023200404A2 (en) 2023-10-19

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