WO2023189782A1 - Plant disease control composition, preparation, and plant disease control method - Google Patents

Abstract

A plant disease control composition containing tebufloquin and an agent for inducing plant resistance as active ingredients.

Description

Plant disease control compositions, formulations, and plant disease control methods

The present disclosure relates to a plant disease control composition, a formulation, and a plant disease control method.

Rice blast is the most damaging disease in rice cultivation caused by the rice blast fungus (Pyricularia oryzae), and many control agents using fungicidal compounds have been reported so far.

In rice blast, many bacteria that are resistant to existing fungicidal compounds have appeared, and there are an increasing number of fields in which a single fungicidal component cannot provide sufficient control effects (Non-Patent Document 1).
Furthermore, although there are some disclosures about compositions using tebufloquine, which is one of the bactericidal ingredients (for example, Patent Documents 1 to 5), there is no disclosure whatsoever about a mixture using tebufloquine and a resistance inducer in combination. .

Patent Document 1: Japanese Patent Application Publication No. 2003-55114 Patent Document 2: International Publication No. 2004/039156 Patent Document 3: International Publication No. 2006/106811 Patent Document 4: Japanese Patent Application Publication No. 2011-201858 Patent Document 5: Japanese Patent Application Publication No. 2011-201858 Publication No. 2007-112760

Non-patent document 1: Latest trends in drug-resistant bacteria research and strengthening of countermeasures, Journal of the Japanese Society of Pesticides, No. 39, Volume 1, pp. 53-57, 2014

In view of the above, there is an increasing demand for fungicides with multiple activities.
Furthermore, it has been found that as a measure to prevent further emergence of fungicide-resistant bacteria, it is preferable to mix substances with different activities to control plant diseases. Furthermore, from the perspective of reducing the environmental burden caused by pesticides, it has been found that it is preferable to utilize resistance inducers that are highly effective in controlling plant diseases by increasing the resistance that plants themselves have.

The problem to be solved by an embodiment of the present disclosure is to exhibit an excellent control effect on plant diseases compared to a plant disease control composition containing tebufloquine alone as an active ingredient, and to reduce the amount of the drug applied. An object of the present invention is to provide a plant disease control composition that reduces environmental burden.
A problem to be solved by other embodiments of the present disclosure is to provide a formulation using a plant disease control composition and a method for controlling plant diseases.

The present inventors have discovered that tebufloquine and a plant resistance inducer exhibit synergistic plant disease control effects, and have completed the present invention.
That is, specific means for solving the problem include the following aspects.

(1) A plant disease control composition containing tebufloquine and a plant resistance inducer as active ingredients.
(2) The plant disease control composition according to (1), wherein the plant disease is rice blast disease.
(3) The plant resistance inducer is jasmonic acid, salicylic acid, probenazole, isotianil, tiadinyl, acibenzolar S-methyl, dichlorbenziazox, laminarin, Japanese knotweed extract, Bacillus mycoides isolate J, Saccharomyces cerevisiae LAS117, The plant disease control composition according to (1), comprising at least one selected from fosetyl, phosphorous acid or a salt thereof, and a compound represented by the following formula (I).

In formula (I), X ₁ and X ₄ each independently represent a hydrogen atom, a fluorine atom, a chlorine atom, or a trifluoromethyl group, and X ₂ and X ₃ each independently represent a hydrogen atom, a fluorine atom, Represents a chlorine atom or a methyl group, A is a halogen atom, a hydroxyl group, an amino group, a methylthio group, an alkyl group having 1 to 4 carbon atoms optionally substituted with 1 to 3 halogen atoms, 1 to 3 Alkyloxy group having 1 to 4 carbon atoms which may be substituted with a halogen atom, alkylcarbonyl group having 1 to 4 carbon atoms, methoxycarbonyl group, ethoxycarbonyl group, benzylaminocarbonyl group, acetoxy group, nitro group, cyano group represents a phenyl group optionally substituted with 1 to 5 groups selected from the group consisting of a phenoxy group, a phenoxy group, and a benzyl group, B represents an oxygen atom or a sulfur atom, and Q represents the formula: -NH- or Represents a divalent group represented by -O-.

(4) A formulation comprising the plant disease control composition according to any one of (1) to (3).
(5) From wettable powders, emulsions, flowables, hydrated granules, dry flowables, powders, DL powders, FD powders, microcapsules, granules, powder granules, jumbo granules, packs, and aerosols. The formulation according to (4) is selected.
(6) A method for controlling plant diseases, which comprises applying the plant disease control composition according to any one of (1) to (3) to plant diseases.
(7) The method for controlling a plant disease according to (6), wherein the plant disease is a rice blast disease.
(8) The method for controlling plant diseases according to (6) or (7), wherein the application includes at least one of foliage spraying and seedling box treatment.

According to an embodiment of the present disclosure, compared to a plant disease control composition containing tebufloquine alone as an active ingredient, it exhibits superior control effects against plant diseases, and also reduces the amount of application of the drug and causes environmental burden. Provided is a plant disease control composition that aims at alleviating.
According to other embodiments of the present disclosure, formulations and methods for controlling plant diseases using plant disease control compositions are provided.

Embodiments of the present disclosure will be described below. Note that these descriptions and examples are provided only for the purpose of illustrating the embodiments of the present disclosure, and do not limit the scope of the present disclosure.

In this specification, "~" indicating a numerical range is used to include the numerical values written before and after it as the lower limit and upper limit.

In the numerical ranges described step by step in this specification, the upper limit value or lower limit value described in one numerical range may be replaced with the upper limit value or lower limit value of another numerical range described step by step. good. Further, in the numerical ranges described in this specification, the upper limit or lower limit of the numerical range may be replaced with the value shown in the Examples.

In this specification, if there are multiple types of substances corresponding to each component in the target object, unless otherwise specified, the amount of each component in the target object is the amount of the multiple types of substances present in the target object. means the total content rate or amount of

One embodiment of the present disclosure relates to a plant disease control composition containing tebufloquine and a plant resistance inducer as active ingredients.

Plant resistance inducers as used herein are substances that do not exhibit direct antibacterial activity against plant pathogens, or substances that exhibit some antibacterial activity but whose activity is not the main activity against plant pathogens that require control. means. More specifically, a plant resistance inducer refers to a substance classified by the Fungicide Resistance Control Committee (FRAC) as mode of action P (induction of host plant resistance) in the FRAC code table.

Although the mechanism by which the plant disease control composition according to the present disclosure exerts its effects is not necessarily clear, it is presumed as follows.
The plant disease control composition according to the present disclosure improves fungicidal action and induces resistance by applying a fungicide that does not have a sufficient control effect on plant diseases alone in combination with a compound having a different action. It has a synergistic effect, can efficiently control plant diseases such as rice blast, and can reduce the number of times of spraying of fungicidal agents. Moreover, the plant disease control composition according to the present disclosure can reduce the risk of emergence of fungicide-resistant bacteria. Furthermore, the plant disease control composition according to the present disclosure can reduce the amount of fungicide applied per cropping season, thereby reducing the environmental load.

The plant disease control composition according to the present disclosure makes a significant contribution to stabilizing agricultural production and protecting the environment.

The plant resistance inducers in the present disclosure include jasmonic acid, salicylic acid, probenazole, isotianil, tiadinyl, acibenzolar S-methyl, dichlorbenziazox, laminarin, Japanese knotweed extract, Bacillus mycoides isolate J, Saccharomyces cerevisiae LAS117, Examples include, but are not limited to, fosetyl, phosphorous acid and its salts, and the compound represented by the following formula (I).

In formula (I), X ₁ and X ₄ each independently represent a hydrogen atom, a fluorine atom, a chlorine atom, or a trifluoromethyl group, and X ₂ and X ₃ each independently represent a hydrogen atom, a fluorine atom, Represents a chlorine atom or a methyl group, A is a halogen atom, a hydroxyl group, an amino group, a methylthio group, an alkyl group having 1 to 4 carbon atoms optionally substituted with 1 to 3 halogen atoms, 1 to 3 Alkyloxy group having 1 to 4 carbon atoms which may be substituted with a halogen atom, alkylcarbonyl group having 1 to 4 carbon atoms, methoxycarbonyl group, ethoxycarbonyl group, benzylaminocarbonyl group, acetoxy group, nitro group, cyano group represents a phenyl group which may be substituted with 1 to 5 groups selected from the group consisting of a phenoxy group and a benzyl group, B represents an oxygen atom or a sulfur atom, and Q represents the formula: -NH- or Represents a divalent group represented by -O-.

A specific example of the compound represented by formula (I) is 2,6-difluoro-N-(3-methoxyphenyl)-4-Pyridinecarboxamide (CAS number: 2244744-34-9. Hereinafter also referred to as compound A). can be mentioned.

Preferred plant resistance inducers include probenazole, isotianil, thiazinyl, acibenzolar S-methyl, dichlorbenziazox, laminarin, and 2,6-difluoro-N-(3-methoxyphenyl)-4-pyridinecarboxamide (CAS number: 2244744). -34-9).

The mixing ratio of tebufloquine and plant resistance inducer in the plant disease control composition according to the present disclosure is preferably in the following range.
When the plant resistance inducer combined with tebufloquine is probenazole, isotianil, tiazinil, dichlorbenziazox, acibenzolar S-methyl, or a compound represented by formula (I), the mixing ratio by mass is 1:1:1: Therefore, it is preferable that the amount of the plant resistance inducer to be combined is 0.0001 to 10.
In addition, when the plant resistance inducer to be combined with tebufloquine is salicylic acid, jasmonic acid, laminarin, Japanese knotweed extract, Bacillus mycoides isolate J, Saccharomyces cerevisiae LAS117, fosetyl, or phosphorous acid or its salt, the mass ratio The mixing ratio is preferably 1:1 for tebufloquine and 0.0001 to 100:0 for the combined plant resistance inducer.

More preferably, the mixing ratio by mass ratio is such that when the plant resistance inducer to be combined with tebufloquine is probenazole, isotianil, tiazinyl, dichlorbenziazox, acibenzolar S-methyl, or a compound represented by formula (I), tebufloquine is mixed with tebufloquine. is 1 to 0.01 to 10 for the combined plant resistance inducer, and when the plant resistance inducer to be combined with tebufloquine is laminarin, the ratio for tebufloquin is 1 to 0.1 to 10 for laminarin.

Examples of plant pathogens to be controlled by the plant disease control composition according to the present disclosure include fungi, bacteria, viruses, and the like. However, it is not limited to these.
Examples of fungi include plant pathogenic fungi, examples of bacteria include plant pathogenic bacteria, and examples of viruses include plant pathogenic viruses.

Examples of plant pathogenic fungi include Alternaria alternata, Alternaria kikutiana, Botrytis cinerea, and Cochliobolus cinerea.
miyabeanus), potato anthracnose (Colletotrichum atramentarium), cucumber anthracnose (Colletotrichum lagenarium), cucumber vine splitting fungus (Fusarium oxysporum f.
sp. lycopersici, Gibberella fujikuroi, Glomerella cingulata, and Glomerella cingulata. Pyricularia oryzae, Rhizoctonia solani), Sclerotinia minor tomato, Verticillium albo-atrum, Puccinia recndi
ta), Erysiphe graminis, Phytophthora infestans, Pseudoperonospora cubensis, Sphaerotheca fuliginea), rice seedling damping-off fungus (Pythium graminicola), tomato ring Alternaria solani, Vegetable sclerotiorum, Venturia inaequalis, Monilinia fructicola, Strawberry anthracnose Colletotrichum gloeosporioides, soybean purpura (Cercospora kikuchii) , Cercospora beticola, Leptosphaeria nodorum, Blumeria graminis, Thanatephorus cucumeris (Fra nk) Donk).

Among the above-mentioned plant pathogenic fungi, preferred plant pathogenic fungi for which the plant disease control composition according to the present disclosure has a particularly good effect as a control target include rice sesame leaf blight fungi, rice bakanae fungi, and rice blast fungi. , rice seedling blight disease fungi, and rice sheath blight disease fungi, and more preferred plant pathogenic fungi include rice blast fungi.

In addition, examples of plant pathogenic bacteria include Pseudomonas, Erwinia, Pectobacterium, Xanthomonas, Burkholderia, Streptomyces, Ralstonia, Clavibacter, and Rhizoma. nas genus, Agrobacterium genus, Bacillus genus, Clostridium genus, Curtobacterium genus, Pantoea Examples include bacteria of the genera Acidovorax, Arthrobacter, and Rhodococcus.

Furthermore, examples of plant pathogenic viruses include, for example, Soil-borne wheat mosaic virus, Soybean mosaic virus, Alfalfa mosaic virus, and Potato leaf curl virus. (Potato leaf roll virus), Examples include Cucumber mosaic virus and Tobacco mosaic virus.

When using the plant disease control composition according to the present disclosure as a formulation, the plant disease control composition according to the present disclosure may be used as is.
Further, a preparation containing the plant disease control composition according to the present disclosure can be made into any dosage form according to a conventional method.

The dosage form of the preparation may be a wettable powder, an emulsion, a flowable preparation, a granule wettable powder, a dry flowable preparation, a powder, a DL powder, an FD powder, or a microcapsule.

Preferably, the formulation is an agrochemical formulation used as an agrochemical.

Here, DL powder refers to a formulation that suppresses scattering and has an average particle size of 20 μm or more and 20% or less of particles with a particle size of 10 μm or less. Furthermore, FD powder is a formulation that utilizes scattering and has an average particle size of 5 μm or less.

The plant disease prevention composition according to the present disclosure can further be used in all formulations such as granules, powders, jumbo granules, packs, and aerosols for use in water in Hondas and seedling boxes.

These preparations can be manufactured by adding agriculturally and horticulturally acceptable additives, if necessary.
Agro-horticulturally acceptable additives include carriers, surfactants, binders, thickeners, antifreeze agents, colorants, adjuvants, and the like.
Carriers include solid carriers, liquid carriers, and gaseous carriers.
Agrochemical formulations can be produced together with these additives by common production methods.

Examples of solid carriers include clays (clay, carrion, diatomaceous earth, bentonite, acid clay, etc.), synthetic hydrated silicon dioxide, talc, perlite, vermiculite, pumice, and other inorganic minerals (sericite, quartz, sulfur, activated carbon, Examples include fine powders and granules such as calcium carbonate, hydrated silica, titanium oxide, etc., synthetic polymers such as starch, lactose, vinyl chloride polymers, and polyurethane, and plant-derived materials such as wood flour.

Examples of liquid carriers include alcohols (methanol, ethanol, isopropanol, polyethylene glycol, propylene glycol, dipropylene glycol, tripropylene glycol, glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), aromatic hydrocarbons (benzyl Alcohol, benzene, toluene, xylene, ethylbenzene, methylnaphthalene, etc.), aliphatic hydrocarbons (paraffin, n-hexane, cyclohexane, kerosene, kerosene, etc.), ethers (diethylene glycol monoethyl ether, diethylene glycol monomethyl ether, diisopropyl ether, diethyl ether, dioxane, tetrahydrofuran, etc.), esters (propylene carbonate, ethyl acetate, butyl acetate, benzyl benzoate, isopropyl myristate, propylene glycol fatty acid esters, etc.), nitriles (acetonitrile, isobutyronitrile, etc.), amides (dimethylformamide, dimethylacetamide, N-methylpyrrolidone, etc.), halogenated hydrocarbons (dichloromethane, trichloroethane, carbon tetrachloride, etc.), animal and vegetable oils such as soybean oil and cottonseed oil, dimethyl sulfoxide, silicone oil, higher fatty acids, Examples include glycerol formal and water.

Examples of gaseous carriers include LPG, air, nitrogen, carbon dioxide, and dimethyl ether.

The content of the carrier used in the plant disease control composition according to the present disclosure is usually 5% by mass to 99.9% by mass, preferably 10% by mass to 98% by mass. be.

Surfactants are used in a variety of applications, including emulsification, dispersion, penetration, and disintegration.
As the surfactant, nonionic surfactants, anionic surfactants, etc. can be used.

As the nonionic surfactant, polyoxyethylene alkyl ethers, polyoxyethylene styrylphenyl ethers, polyoxyethylene/polyoxypropylene Brook copolymers, (polyoxyethylene) sorbitan fatty acid esters, etc. are used.
As the anionic surfactant, alkyl sulfate esters, alkyl (aryl) sulfonates, naphthalene sulfonic acid/formalin condensates, lignin sulfonates, polycarboxylic acid salts, etc. are used.

The amount of surfactant used in the plant disease control composition according to the present disclosure is usually 0.01% to 30% by mass, preferably 0.05% to 20% by mass. Mass%.

As the binder, cellulose binders such as carboxymethyl cellulose salts and methyl cellulose, starch binders such as polyvinyl alcohol, gum arabic, and dextrin are used.
As the thickener, an organic polymer thickener such as xanthan gum or an inorganic powder such as bentonite is used.
As the anti-freezing agent, a glycol-based anti-freezing agent such as propylene glycol is used.
As the coloring agent, for example, tar dyes such as Blue No. 1 and Red No. 2, and caramel dyes are used.
Furthermore, adjuvants can be added for the purpose of improving the physical properties of the formulation. For example, white carbon, calcium stearate, and isothiazolin-3-one derivatives are used as fungicides to improve fluidity.

In the formulation containing the plant disease control composition according to the present disclosure, the content in the formulation is not limited, but the content of the plant disease control composition in 100 parts by mass of the formulation is in the range of 0.01 part by mass to 90 parts by mass. The mass part can be set appropriately from . The preferred content of the plant disease control composition in the formulation is in the range of 0.1 parts by mass to 50 parts by mass.

The plant disease control composition according to the present disclosure does not limit the application amount, but includes, for example, the weather, environmental conditions, cultivation conditions, growth conditions, application timing, application method, and disease onset of the target area for plant disease control. It is possible to appropriately set the application amount depending on disease-causing factors such as the severity of the disease.
The effective amount of the plant disease control composition to be applied is preferably 0.01 g to 10 kg, more preferably 1 g to 5 kg per 10 ares.

The plant disease control composition according to the present disclosure can be used during all crop cultivation periods in which pesticides are normally used, that is, from pre-sowing to germination, seedling-raising, vegetative growth, and pre-harvesting. be able to.
A preferred method of using the plant disease control composition according to the present disclosure is that it can be used at any time during the rice cultivation period, from after germination to the seedling-raising period, rice cultivation, and before harvest. A more preferred method of use is from the seedling-raising period to before harvest.

Application of the plant disease control composition according to the present disclosure includes foliage spraying treatment on individual plants, seedling nursery box treatment, side row application using a paddy rice transplanter, water surface application to rice field water, spraying treatment on the soil surface, and soil surface treatment. Soil mixing after spraying, injection into the soil, soil mixing after injection into the soil, soil irrigation, soil mixing after soil irrigation, spraying onto plant seeds, mixing into plant seeds Examples include smearing, application by dipping plant seeds, application as a powder coating on plant seeds, mixing with plant seeds, and mixing with fertilizer. Plant diseases can be controlled by these applications, but more preferred methods of use are foliar spraying and seedling box treatment.

The plant disease control composition according to the present disclosure exhibits sufficient efficacy for plant disease control in any application.

The plant disease control composition according to the present disclosure can also be applied with a mixture of pest control agents such as insecticides, fungicides, acaricides, and herbicides.
Furthermore, the plant disease control composition according to the present disclosure and the pest control agent described above can be applied separately at different times.

The above-mentioned insecticides include, for example, acephate, dichlorvos, EPN, fenitrothion, fenamifos, prothiofos, profenofos, pyraclofos. clofos), chlorpyrifos-methyl (chlorpyrifos) -methyl), chlorfenvinphos, demeton, ethion, malathion, coumaphos, isoxathion, fenthion, diazione diazinon, thiodicarb ), aldicarb, oxamyl, propoxur, carbaryl, fenobucarb, ethiofencarb, phenothiocarb, pirimicarb ( pirimicarb), carbofuran, carbosulfan (carbosulfan), furathiocarb, hyquincarb, alanycarb, methomyl, benfuracarb, cartap, thiocyc lam), bensultap, dicofol, tetradifon (tetradifon), acrinathrin, bifenthrin, cycloprothrin, cyfluthrin, dimefluthrin, empenthrin , fenfluthrin, fenpropathrin, imiprothrin, metofluthrin, permethrin, phenothrin, resmethrin, tefluthrin, tetramethrin rin), tralomethrin, transfluthrin, cypermethrin ( cypermethrin, deltamethrin, cyhalothrin, fenvalerate, fluvalinate, ethofenprox, flufenprox ), halfenprox, silafluofen , cyromazine, diflubenzuron, teflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron uron), lufenuron, novaluron, penfluron ( penfluron, triflumuron, chlorfluazuron, diafenthiuron, methoprene, fenoxycarb, pyriproxyfen roxyfen), halofenozide, tebufenozide ), methoxyfenozide, chromafenozide, dicyclanil, buprofezin, hexythiazox, amitraz z), chlordimeform, pyridaben, fenpyroximate, flufenerim ), pyrimidifen, tebufenpyrad, tolfenpyrad, fluacrypyrim, acequinocyl, cyflumetofen, fulvene flubendiamide, ethiprole, fipronil, etoxazole ( ethoxazole), imidacloprid, nitenpyram, clothianidin, acetamiprid, dinotefuran, thiacloprid rid), thiamethoxam, pymetrozine, bifenazate, spirodiclo spirodiclofen, spiromesifen, flonicamid, chlorfenapyr, pyriproxyfene, indoxacarb, pyridalyl spinosad, avermectin , milbemycin, azadirachtin, nicotine, rotenone, BT bacterium, BT protein, entomopathogenic virus, emamectinbenzoate, spinetora m), pyrifluquinazone, chlor chlorantraniliprole, cyantraniliprole, cyenopyrafen, spirotetramat, lepimectin, metaflu mizone), pyrafluprole, pyriprole, Examples include dimefluthrin, fenazaflor, hydramethylnon, triazamate, afidopyropen, flupyrimin.

The above-mentioned fungicides include, for example, strobosides such as azoxystrobin, kresoxim-methyl, trifloxystrobin, metominostrobin, and orysastrobin. Bilurin compound; mepanipyrim Anilinopyrimidine compounds such as mepanipyrim, pyrimethanil, cyprodinil; triadimefon, bitertanol, triflumizole, meth Conazole (metconazole), propiconazole (propiconazole) , penconazole, flusilazole, myclobutanil, cyproconazole, tebuconazole, hexaconazole Azole compounds such as zole, prochloraz, and simeconazole ; quinoxaline compounds such as chinomethionate; dithiocarbamate compounds such as maneb, zineb, mancozeb, polycarbamate, propineb substance; diethofencarb phenyl carbamate compounds such as; organochlorine compounds such as chlorothalonil, quintozene; Benzimidazole compounds; metalaxyl Phenylamide compounds such as (metalaxyl), oxadixyl, ofurace, benalaxyl, furaxyl, cyprofuram; Sulfenic acid compounds such as; Copper-based compounds such as cupric hydroxide and oxyquinoline copper; isoxazole-based compounds such as hydroxyisoxazole; fosetyl-aluminum, tolclofos-methyl ( Organophosphorus compounds such as tolclofos-methyl; N-haloalkylthio compounds such as captan, captafol, and folpet; procymidone, iprodione, and vinclozolin. zolin ); dicarboximide compounds such as flutolanil, mepronil, furametpyr, thifluzamide, boscalid, penthiopyrad; Xyanilide compound; fenpropimorph Morpholine compounds such as (fenpropimorph) and dimethomorph; Organotin compounds such as triphenyltin hydroxide and fentin acetate; Fludioxon il), fenpiclonil ) and their agriculturally acceptable acid addition salts.

Additionally, other fungicide compounds include tricyclazole, pyroquilon, carpropamide, diclocymet, fenoxanil, fthalide, fluazinam (f luazinam), cymoxanil, triforine (triforine), pyrifenox, fenarimol, fenpropidin, pencycuron, ferimzone, cyazofamid, iprovaricarb (i) provalicarb), benthiavalicarb isopropyl (benthiavalicarb) -isopropyl), iminoctadin albesilate, cyflufenamide, kasugamycin, validamycin, streptomycin , oxolinic acid, tebufloquin, isoprothiolane , tolprocarb, pydiflumetofen, picarbutrazox, mandestrobin, dipymethitrone, pyrazifl umid), oxathiapipropyline, penflufen ( penflufen), fluoxapiprolin, florylpicoxamide, fluopimomide, ipflufenoquin, fluindapyr , isoflucypram, quinofumelin, Examples include methyltetraprole, pyridachlomethyl, pyrapropoyne, aminopyrifen, inpyrfluxam, and agriculturally acceptable acid addition salts thereof.

Examples of the acaricides mentioned above include bromopropylate, tetradifon, propargite, amitraz, phenothiocarb, hexythiazox, fenbutatin oxide , dienochlor, fenpyroximate, tebufenpyrad,
Pyridaben, pyrimidifen, clofentezine, etoxazole, halfenprox, milbemectin, acequinocyl inocyl), bifenazate, fluacrypyrim, spiro Examples include spirodichlorofen, spiromesifen, chlorfenapyr, avermectin, cyenopyrafen, and cyflumetofen.

In addition, the above herbicides include, for example, phenoxy acid compounds such as cyhalofop-butyl and 2,4-D; carbamate compounds such as esprocarb and desmedipham; Acid amide compounds such as alachlor and metolachlor; Urea compounds such as diuron and tebuthiuron; furon) etc. Sulfonylurea compounds; pyrimidyloxybenzoic acid compounds such as pyriminobac-methyl; glufosinate salts (glufosinate) such as glyphosate, bilanafos, glufosinate, glufosinate ammonium Amino acid systems such as fosinate salt) Examples include compounds.

The plant disease control composition according to the present disclosure can also be applied after being mixed with a plant growth regulator or fertilizer. Moreover, the plant disease control composition of the present disclosure and the above-mentioned plant growth regulator or fertilizer can be applied separately at different times.

The above plant growth regulators include, for example, ethylene agents such as ethephon; auxin agents such as indolebutyric acid and ethychlozate; cytokinin agents; gibberellin agents; auxin antagonists; dwarfing agents; Examples include transpiration inhibitors.

In addition, the above-mentioned fertilizers include, for example, nitrogenous fertilizers such as urea, ammonium nitrate, ammonium nitrate, and ammonium chloride; Potassium fertilizers such as potassium chloride, potassium bicarbonate, potassium nitrate, potassium nitrate, potassium sodium nitrate; Manganese fertilizers such as manganese sulfate and manganese nitrate; Boron fertilizers such as boric acid and borates can be mentioned.

Hereinafter, the present disclosure will be specifically explained with reference to formulation examples and test examples, but the present disclosure is not limited to these formulation examples and test examples.

(Formulation Example 1) Wettable powder: 2% by mass of tebufloquine, 20% by mass of probenazole, 58% by mass of clay, 2% by mass of white carbon, 13% by mass of diatomaceous earth, 4% by mass of calcium ligninsulfonate, 1% by mass of sodium lauryl sulfate. The mixture was uniformly mixed and ground to obtain a wettable powder.

(Formulation Example 2) Granule wettable powder Uniformly mixed and pulverized 2% by mass of tebufloquine, 20% by mass of isotianil, 68% by mass of clay, 5% by mass of dextrin, 4% by mass of alkylmaleic acid copolymer, and 1% by mass of sodium lauryl sulfate. After adding water and kneading well, the mixture was granulated and dried to obtain a granular wettable powder.

(Formulation Example 3) Flowable agent tebufloquine 3% by mass, diclobentiazox 30% by mass, polyoxyethylene styrylphenyl ether sulfate 5% by mass, propylene glycol 6% by mass, bentonite 1% by mass, silicone antifoaming agent (product Name: PRONAL EX-300, Toho Chemical Industry Co., Ltd.) 0.05% by mass, antifungal agent (Product name: Biohop L, K.I. Kasei Co., Ltd.) 0.
After mixing 0.02% by mass, an appropriate amount of water was added and the mixture was pulverized using a wet pulverizer. Thereafter, 3% by mass of a 1% xanthan gum aqueous solution and the remaining water were added to make 100% by mass to obtain a flowable agent.

(Formulation Example 4) Emulsion 9.2% by mass of tebufloquine, 5.8% by mass of 2,6-difluoro-N-(3-methoxyphenyl)-4-pyridinecarboxamide (compound A), 20% by mass of N,N-dimethylformamide %, aromatic solvent (trade name: T-SOL 150, ENEOS Corporation), 55% by mass, and polyoxyethylene alkylaryl ether 10% by mass were uniformly mixed and dissolved to obtain an emulsion.

(Formulation example 5) Dry flowable agent 10% by mass of tebufloquine, 10% by mass of acibenzolar S-methyl, 65% by mass of kaolin clay, 5% by mass of dextrin, 5% by mass of alkylmaleic acid copolymer, 5% by mass of sodium lauryl sulfate uniformly After mixing and pulverizing the mixture, adding water and kneading well, the mixture was granulated and dried to obtain a dry flowable agent.

(Formulation Example 6) Powder 0.5% by mass of tebufloquine, 5.0% by mass of tiadinil, 57.5% by mass of clay, 36% by mass of talc, and 1% by mass of calcium stearate were uniformly mixed to obtain a powder.

(Formulation Example 7) DL powder Tebufloquine 1% by mass, laminarin 1% by mass, DL clay (trade name: Showa DL Clay, Showa KDE Co., Ltd.) 95.5% by mass, white carbon 2% by mass, light liquid paraffin 0. A DL powder was obtained by uniformly mixing 5% by mass.

(Test Example 1) Preventive spraying treatment against rice blast The test solution was prepared by dissolving tebufloquine and each resistance inducer listed in Table 1 in acetone. This acetone solution was diluted with water to the concentration listed in Table 1, and 1/1,000 volume of a spreading agent (Neoesterin, manufactured by Kumiai Chemical Industry Co., Ltd.) was added to form a spray solution. .

Eight to ten seeds of rice (variety: Jukoku) were planted in small pots with a diameter of 3 cm and cultivated in cultivation soil in a greenhouse. 2 mL of the spray solution prepared as described above was sprayed on seedlings at the 2-leaf or 3-leaf stage and allowed to air dry.

On the other hand, conidia of rice blast (Pyricularia oryzae) cultured on an agar medium were suspended at a concentration of 1 x 10 ⁵ cells/mL to 5 x 10 ⁵ cells/mL. For this suspension, a solution obtained by diluting Tween 20 4000 times with deionized water was used. This rice blast conidia suspension was spray inoculated into rice plants and infected in a dark humid room for 24 hours. Thereafter, rice was cultivated in a greenhouse at 25°C/18°C day and night, and the control value was calculated using the following formula based on the number of blast lesions that appeared on rice leaves 7 to 9 days after inoculation. Then, the presence or absence of a synergistic effect was tested using Colby's calculation formula below.

Control value = ((number of lesions in untreated area - number of lesions in treated area) / number of lesions in untreated area) x 100

(Colby calculation formula)
Theoretical value = (Preventive value A + Preventive value B) - (Preventive value A × Preventive value B) / 100

The results shown in Table 1 below show that the actual control value exceeds Colby's theoretical value, demonstrating a synergistic effect.

(Test Example 2) Long-term control effect on rice blast The test solution was prepared by dissolving tebufloquine and Compound A in acetone. This acetone solution was diluted with water to the concentration listed in Table 2, and 1/1,000 volume of a spreading agent (Neoesterin, manufactured by Kumiai Chemical Industry Co., Ltd.) was added to form a spray solution. .
15 g of rice (variety: Jukoku) seeds were sown in a small vat measuring 30 cm x 20 cm, and cultivated in a greenhouse at 25° C./18° C. day and night.

On the other hand, conidia of rice blast fungus (Pyricularia oryzae) cultured on an agar medium were suspended at a concentration of 1×10 ⁴ cells/mL to 5×10 ⁴ cells/mL. For this suspension, a solution obtained by diluting Tween 20 4000 times with deionized water was used. This rice blast conidia suspension was spray inoculated into two-leaf stage rice seedlings, and infected in a dark humid room for 24 hours. Thereafter, rice seedlings were cultivated in a greenhouse, and blast lesions were observed on the rice leaves 7 to 9 days after inoculation.
12 mL of the above spray solution was sprayed and air-dried indoors. Thereafter, it was left in a wet room (temperature: 25°C, humidity: 100%) for 24 hours, and the rice blast fungus was naturally infected in the vat.
After another week, the cells were naturally infected for 24 hours. After that, rice seedlings were cultivated for 7 to 9 days in a greenhouse set at 25°C/18°C during the day and night, and rice blast disease formed on the expanded uppermost leaves of 50 randomly selected seedlings. The number of spots was measured. Based on the number of lesions obtained, the control value was calculated using the following formula. Then, the presence or absence of a synergistic effect was tested using Colby's formula.

Control value = ((number of lesions in untreated area - number of lesions in treated area) / number of lesions in untreated area) x 100

(Colby's theoretical formula)
Theoretical value = (Preventive value A + Preventive value B) - (Preventive value A × Preventive value B) / 100

The results shown in Table 2 below show that the actual control value greatly exceeds Colby's theoretical value, confirming a synergistic effect.

The plant disease control composition of the present disclosure has excellent control effects against rice blast, further reduces the risk of resistant bacteria, and is a useful composition for agricultural use.

The disclosure of Japanese Patent Application No. 2022-052593 filed on March 28, 2022 is incorporated herein by reference in its entirety. In addition, all documents, patent applications, and technical standards mentioned herein are incorporated by reference to the same extent as if each individual document, patent application, and technical standard were specifically and individually indicated to be incorporated by reference. , herein incorporated by reference.

Claims (8)

1. A plant disease control composition containing tebufloquine and a plant resistance inducer as active ingredients.
2. The plant disease control composition according to claim 1, wherein the plant disease is blast disease.
3. The plant resistance inducers include jasmonic acid, salicylic acid, probenazole, isotianil, thiazinyl, acibenzolar S-methyl, dichlorbenziazox, laminarin, Japanese knotweed extract, Bacillus mycoides isolate J, Saccharomyces cerevisiae LAS117, fosetyl, The plant disease control composition according to claim 1, comprising at least one selected from phosphoric acid or a salt thereof and a compound represented by the following formula (I).
   
   
   [In formula (I), X ₁ and X ₄ each independently represent a hydrogen atom, a fluorine atom, a chlorine atom, or a trifluoromethyl group, and X ₂ and X ₃ each independently represent a hydrogen atom, a fluorine atom , represents a chlorine atom or a methyl group, A is a halogen atom, a hydroxyl group, an amino group, a methylthio group, an alkyl group having 1 to 4 carbon atoms optionally substituted with 1 to 3 halogen atoms, 1 to 3 an alkyloxy group having 1 to 4 carbon atoms which may be substituted with halogen atoms, an alkylcarbonyl group having 1 to 4 carbon atoms, a methoxycarbonyl group, an ethoxycarbonyl group, a benzylaminocarbonyl group, an acetoxy group, a nitro group, Represents a phenyl group optionally substituted with 1 to 5 groups selected from the group consisting of a cyano group, a phenoxy group, and a benzyl group, B represents an oxygen atom or a sulfur atom, and Q has the formula: -NH- Or represents a divalent group represented by -O-. ]
4. A preparation comprising the plant disease control composition according to any one of claims 1 to 3.
5. Selected from wettable powders, emulsions, flowables, wettable powders, dry flowables, powders, DL powders, FD powders, microcapsules, granules, powder granules, jumbo granules, packs, and aerosols. The formulation according to claim 4.
6. A method for controlling plant diseases, which comprises applying the plant disease control composition according to any one of claims 1 to 3 to plant diseases.
7. The method for controlling a plant disease according to claim 6, wherein the plant disease is a rice blast disease.
8. The method for controlling plant diseases according to claim 6 or 7, wherein the application includes at least one of foliage spraying and seedling box treatment.