WO2023178098A3 - Engineered plants having modified inositol pyrophosphates - Google Patents

Engineered plants having modified inositol pyrophosphates Download PDF

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Publication number
WO2023178098A3
WO2023178098A3 PCT/US2023/064331 US2023064331W WO2023178098A3 WO 2023178098 A3 WO2023178098 A3 WO 2023178098A3 US 2023064331 W US2023064331 W US 2023064331W WO 2023178098 A3 WO2023178098 A3 WO 2023178098A3
Authority
WO
WIPO (PCT)
Prior art keywords
engineered plants
inositol pyrophosphates
modified
modified inositol
pyrophosphates
Prior art date
Application number
PCT/US2023/064331
Other languages
French (fr)
Other versions
WO2023178098A2 (en
Inventor
Glenda GILLASPY
Catherine FREED
Caitlin CRIDLAND
Branch CRAIGE
Original Assignee
Virginia Polytechnic Institute And State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Virginia Polytechnic Institute And State University filed Critical Virginia Polytechnic Institute And State University
Publication of WO2023178098A2 publication Critical patent/WO2023178098A2/en
Publication of WO2023178098A3 publication Critical patent/WO2023178098A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Described in certain example embodiments herein are engineered plants that overexpress an ITPK and/or a VIP gene, gene product, or both. In some embodiments, the VIP gene, gene product, or both is the kinase domain of the an ITPK gene product. Also described in certain example embodiments herein are methods of making and uses of the engineered plants described herein.
PCT/US2023/064331 2022-03-14 2023-03-14 Engineered plants having modified inositol pyrophosphates WO2023178098A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263319679P 2022-03-14 2022-03-14
US63/319,679 2022-03-14

Publications (2)

Publication Number Publication Date
WO2023178098A2 WO2023178098A2 (en) 2023-09-21
WO2023178098A3 true WO2023178098A3 (en) 2023-11-30

Family

ID=88024501

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/064331 WO2023178098A2 (en) 2022-03-14 2023-03-14 Engineered plants having modified inositol pyrophosphates

Country Status (1)

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WO (1) WO2023178098A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090081354A1 (en) * 2001-09-27 2009-03-26 Pioneer Hi-Bred Interntional, Inc. Phytate Polynucleotides and Methods of Use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090081354A1 (en) * 2001-09-27 2009-03-26 Pioneer Hi-Bred Interntional, Inc. Phytate Polynucleotides and Methods of Use

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ADEPOJU OLUSEGUN, WILLIAMS SARAH P., CRAIGE BRANCH, CRIDLAND CAITLIN A., SHARPE AMANDA K., BROWN ANNE M., LAND ERIC, PERERA IMARA : "Inositol Trisphosphate Kinase and Diphosphoinositol Pentakisphosphate Kinase Enzymes Constitute the Inositol Pyrophosphate Synthesis Pathway in Plants", BIORXIV, 5 August 2019 (2019-08-05), XP093115887, Retrieved from the Internet <URL:https://www.biorxiv.org/content/10.1101/724914v1.full.pdf> [retrieved on 20240104], DOI: 10.1101/724914 *
RIEMER ESTHER, QIU DANYE, LAHA DEBABRATA, HARMEL ROBERT K., GAUGLER PHILIPP, GAUGLER VERENA, FREI MICHAEL, HAJIREZAEI MOHAMMAD-REZ: "ITPK1 is an InsP6/ADP phosphotransferase that controls phosphate signaling in Arabidopsis", MOLECULAR PLANT, vol. 14, no. 11, 1 November 2021 (2021-11-01), pages 1864 - 1880, XP093115886, ISSN: 1674-2052, DOI: 10.1016/j.molp.2021.07.011 *

Also Published As

Publication number Publication date
WO2023178098A2 (en) 2023-09-21

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