WO2023147445A3 - Cell-free rna biomarkers for the detection of cancer or predisposition to cancer - Google Patents
Cell-free rna biomarkers for the detection of cancer or predisposition to cancer Download PDFInfo
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- WO2023147445A3 WO2023147445A3 PCT/US2023/061410 US2023061410W WO2023147445A3 WO 2023147445 A3 WO2023147445 A3 WO 2023147445A3 US 2023061410 W US2023061410 W US 2023061410W WO 2023147445 A3 WO2023147445 A3 WO 2023147445A3
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- WIPO (PCT)
- Prior art keywords
- cancer
- predisposition
- biomarkers
- cfrna
- differential expression
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- 206010028980 Neoplasm Diseases 0.000 title abstract 6
- 201000011510 cancer Diseases 0.000 title abstract 6
- 108091092259 cell-free RNA Proteins 0.000 title abstract 5
- 239000000090 biomarker Substances 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title 1
- -1 ZRANB2-AS2 Proteins 0.000 abstract 2
- 238000010195 expression analysis Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 101150037123 APOE gene Proteins 0.000 abstract 1
- 102100022117 Abnormal spindle-like microcephaly-associated protein Human genes 0.000 abstract 1
- 102100029470 Apolipoprotein E Human genes 0.000 abstract 1
- 102000014817 CACNA1A Human genes 0.000 abstract 1
- 102100026679 Carboxypeptidase Q Human genes 0.000 abstract 1
- 102100025832 Centromere-associated protein E Human genes 0.000 abstract 1
- 101100396941 Citrobacter freundii cai gene Proteins 0.000 abstract 1
- 102100028523 Cytoplasmic dynein 1 intermediate chain 2 Human genes 0.000 abstract 1
- 102100027907 Cytoplasmic tyrosine-protein kinase BMX Human genes 0.000 abstract 1
- 102100035890 Delta(24)-sterol reductase Human genes 0.000 abstract 1
- 101000755748 Escherichia coli AIDA-I autotransporter Proteins 0.000 abstract 1
- 102100031758 Extracellular matrix protein 1 Human genes 0.000 abstract 1
- 102100038614 Hemoglobin subunit gamma-1 Human genes 0.000 abstract 1
- 102100038617 Hemoglobin subunit gamma-2 Human genes 0.000 abstract 1
- 102100039271 Histone H2A type 1-H Human genes 0.000 abstract 1
- 102100030690 Histone H2B type 1-C/E/F/G/I Human genes 0.000 abstract 1
- 101000900939 Homo sapiens Abnormal spindle-like microcephaly-associated protein Proteins 0.000 abstract 1
- 101000755749 Homo sapiens Axin interactor, dorsalization-associated protein Proteins 0.000 abstract 1
- 101000910846 Homo sapiens Carboxypeptidase Q Proteins 0.000 abstract 1
- 101000914247 Homo sapiens Centromere-associated protein E Proteins 0.000 abstract 1
- 101000915292 Homo sapiens Cytoplasmic dynein 1 intermediate chain 2 Proteins 0.000 abstract 1
- 101000935548 Homo sapiens Cytoplasmic tyrosine-protein kinase BMX Proteins 0.000 abstract 1
- 101000929877 Homo sapiens Delta(24)-sterol reductase Proteins 0.000 abstract 1
- 101000866526 Homo sapiens Extracellular matrix protein 1 Proteins 0.000 abstract 1
- 101001031977 Homo sapiens Hemoglobin subunit gamma-1 Proteins 0.000 abstract 1
- 101001031961 Homo sapiens Hemoglobin subunit gamma-2 Proteins 0.000 abstract 1
- 101001036100 Homo sapiens Histone H2A type 1-H Proteins 0.000 abstract 1
- 101001084682 Homo sapiens Histone H2B type 1-C/E/F/G/I Proteins 0.000 abstract 1
- 101001034846 Homo sapiens Interferon-induced transmembrane protein 3 Proteins 0.000 abstract 1
- 101000677891 Homo sapiens Iron-sulfur clusters transporter ABCB7, mitochondrial Proteins 0.000 abstract 1
- 101000971521 Homo sapiens Kinetochore scaffold 1 Proteins 0.000 abstract 1
- 101001059802 Homo sapiens N-formyl peptide receptor 3 Proteins 0.000 abstract 1
- 101000991410 Homo sapiens Nucleolar and spindle-associated protein 1 Proteins 0.000 abstract 1
- 101001021103 Homo sapiens Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial Proteins 0.000 abstract 1
- 101000736088 Homo sapiens PC4 and SFRS1-interacting protein Proteins 0.000 abstract 1
- 101000920625 Homo sapiens Protein 4.2 Proteins 0.000 abstract 1
- 101001048702 Homo sapiens RNA polymerase II elongation factor ELL2 Proteins 0.000 abstract 1
- 101000576901 Homo sapiens Serine/threonine-protein kinase MRCK alpha Proteins 0.000 abstract 1
- 101000601441 Homo sapiens Serine/threonine-protein kinase Nek2 Proteins 0.000 abstract 1
- 101000825726 Homo sapiens Structural maintenance of chromosomes protein 4 Proteins 0.000 abstract 1
- 101000844217 Homo sapiens Thioredoxin domain-containing protein 16 Proteins 0.000 abstract 1
- 101000596302 Homo sapiens Transmembrane protein 150C Proteins 0.000 abstract 1
- 101000935117 Homo sapiens Voltage-dependent P/Q-type calcium channel subunit alpha-1A Proteins 0.000 abstract 1
- 101000804798 Homo sapiens Werner syndrome ATP-dependent helicase Proteins 0.000 abstract 1
- 101000781944 Homo sapiens Zinc finger CCCH domain-containing protein 6 Proteins 0.000 abstract 1
- 102100040035 Interferon-induced transmembrane protein 3 Human genes 0.000 abstract 1
- 102100021504 Iron-sulfur clusters transporter ABCB7, mitochondrial Human genes 0.000 abstract 1
- 102100021464 Kinetochore scaffold 1 Human genes 0.000 abstract 1
- 101100135884 Mus musculus Pdia4 gene Proteins 0.000 abstract 1
- 102100028130 N-formyl peptide receptor 3 Human genes 0.000 abstract 1
- 102100030991 Nucleolar and spindle-associated protein 1 Human genes 0.000 abstract 1
- 102100036201 Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial Human genes 0.000 abstract 1
- 102100036220 PC4 and SFRS1-interacting protein Human genes 0.000 abstract 1
- 102100031953 Protein 4.2 Human genes 0.000 abstract 1
- 102100023750 RNA polymerase II elongation factor ELL2 Human genes 0.000 abstract 1
- 102100025352 Serine/threonine-protein kinase MRCK alpha Human genes 0.000 abstract 1
- 102100037703 Serine/threonine-protein kinase Nek2 Human genes 0.000 abstract 1
- 102100022842 Structural maintenance of chromosomes protein 4 Human genes 0.000 abstract 1
- 102100032034 Thioredoxin domain-containing protein 16 Human genes 0.000 abstract 1
- 102100035253 Transmembrane protein 150C Human genes 0.000 abstract 1
- 102100035336 Werner syndrome ATP-dependent helicase Human genes 0.000 abstract 1
- 102100036581 Zinc finger CCCH domain-containing protein 6 Human genes 0.000 abstract 1
- ZPCCSZFPOXBNDL-ZSTSFXQOSA-N [(4r,5s,6s,7r,9r,10r,11e,13e,16r)-6-[(2s,3r,4r,5s,6r)-5-[(2s,4r,5s,6s)-4,5-dihydroxy-4,6-dimethyloxan-2-yl]oxy-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy-10-[(2r,5s,6r)-5-(dimethylamino)-6-methyloxan-2-yl]oxy-5-methoxy-9,16-dimethyl-2-oxo-7-(2-oxoe Chemical compound O([C@H]1/C=C/C=C/C[C@@H](C)OC(=O)C[C@H]([C@@H]([C@H]([C@@H](CC=O)C[C@H]1C)O[C@H]1[C@@H]([C@H]([C@H](O[C@@H]2O[C@@H](C)[C@H](O)[C@](C)(O)C2)[C@@H](C)O1)N(C)C)O)OC)OC(C)=O)[C@H]1CC[C@H](N(C)C)[C@@H](C)O1 ZPCCSZFPOXBNDL-ZSTSFXQOSA-N 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 101150114014 cagA gene Proteins 0.000 abstract 1
- 239000013256 coordination polymer Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
- G16B40/20—Supervised data analysis
Abstract
Methods of detecting or treating cancer or predisposition to cancer are provided, the methods including analyzing a level of one or more cell-free RNA (cfRNA) biomarkers selected from AIDA, CAI, CENPE, CPOX, ELL2, EPB42, HBG1, HBG2, NEK2, NUSAP1, APOE, C3, CP, DHCR24, EGA, FGB, EGG, HRG, IFITM3, ATP IB 1, FPR3, SMC4, TXNDC16, ASPM, WRN, ZRANB2-AS2, BMX, CDC42BPA, KNL1, CACNA1A, ABCB7, HIST1H2BF, PSIP1, TMEM150C, ZC3H6, C9orfl6, CPQ, DYNC1I2, ECM1, and HIST1H2AH, or any combination thereof, in the biological sample; and performing a differential expression analysis comparing the level of each of the one or more cfRNA biomarkers to a corresponding control value (CV); in which differential expression shown by the differential expression analysis between the one or more cfRNA biomarkers and corresponding CVs indicates cancer or a predisposition for cancer in the subject.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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US202263303970P | 2022-01-27 | 2022-01-27 | |
US63/303,970 | 2022-01-27 | ||
US202263426258P | 2022-11-17 | 2022-11-17 | |
US63/426,258 | 2022-11-17 |
Publications (2)
Publication Number | Publication Date |
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WO2023147445A2 WO2023147445A2 (en) | 2023-08-03 |
WO2023147445A3 true WO2023147445A3 (en) | 2023-10-19 |
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PCT/US2023/061410 WO2023147445A2 (en) | 2022-01-27 | 2023-01-26 | Cell-free rna biomarkers for the detection of cancer or predisposition to cancer |
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WO (1) | WO2023147445A2 (en) |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2002074961A1 (en) * | 2001-03-15 | 2002-09-26 | Hyseq, Inc. | Novel nucleic acids and polypeptides |
US20040053248A1 (en) * | 2000-12-22 | 2004-03-18 | Tang Y. Tom | Novel nucleic acids and polypeptides |
US20040171823A1 (en) * | 2003-01-14 | 2004-09-02 | Nadler Steven G. | Polynucleotides and polypeptides associated with the NF-kappaB pathway |
US7462453B2 (en) * | 2004-06-01 | 2008-12-09 | Sumitomo Chemical Company, Limited | Common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene and use thereof |
US20100129874A1 (en) * | 2008-09-05 | 2010-05-27 | The Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
US20160333415A1 (en) * | 2013-01-22 | 2016-11-17 | Otsuka Pharmaceutical Co., Ltd. | QUANTIFICATION METHOD FOR EXPRESSION LEVEL OF WT1 mRNA |
US20180057842A1 (en) * | 2014-11-10 | 2018-03-01 | Murdoch Childrens Research Institute | Vectors and methods for targeted integration in loci comprising constitutively expressed genes |
WO2020092259A1 (en) * | 2018-10-29 | 2020-05-07 | Molecular Stethoscope, Inc. | Characterization of bone marrow using cell-free messenger-rna |
US20210388451A1 (en) * | 2020-06-16 | 2021-12-16 | Grail, Inc. | Methods for analysis of cell-free rna |
-
2023
- 2023-01-26 WO PCT/US2023/061410 patent/WO2023147445A2/en unknown
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
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US20040053248A1 (en) * | 2000-12-22 | 2004-03-18 | Tang Y. Tom | Novel nucleic acids and polypeptides |
WO2002074961A1 (en) * | 2001-03-15 | 2002-09-26 | Hyseq, Inc. | Novel nucleic acids and polypeptides |
US20040171823A1 (en) * | 2003-01-14 | 2004-09-02 | Nadler Steven G. | Polynucleotides and polypeptides associated with the NF-kappaB pathway |
US7462453B2 (en) * | 2004-06-01 | 2008-12-09 | Sumitomo Chemical Company, Limited | Common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene and use thereof |
US20100129874A1 (en) * | 2008-09-05 | 2010-05-27 | The Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
US20160333415A1 (en) * | 2013-01-22 | 2016-11-17 | Otsuka Pharmaceutical Co., Ltd. | QUANTIFICATION METHOD FOR EXPRESSION LEVEL OF WT1 mRNA |
US20180057842A1 (en) * | 2014-11-10 | 2018-03-01 | Murdoch Childrens Research Institute | Vectors and methods for targeted integration in loci comprising constitutively expressed genes |
WO2020092259A1 (en) * | 2018-10-29 | 2020-05-07 | Molecular Stethoscope, Inc. | Characterization of bone marrow using cell-free messenger-rna |
US20210388451A1 (en) * | 2020-06-16 | 2021-12-16 | Grail, Inc. | Methods for analysis of cell-free rna |
Non-Patent Citations (1)
Title |
---|
MATHIAS UHLEN, ZHANG CHENG, LEE SUNJAE, SJöSTEDT EVELINA, FAGERBERG LINN, BIDKHORI GHOLAMREZA, BENFEITAS RUI, ARIF MUHAMMAD, : "A pathology atlas of the human cancer transcriptome", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 357, no. 6352, 18 August 2017 (2017-08-18), US , pages 1 - 13, XP055693994, ISSN: 0036-8075, DOI: 10.1126/science.aan2507 * |
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