WO2023143610A3 - 载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用 - Google Patents

载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用 Download PDF

Info

Publication number
WO2023143610A3
WO2023143610A3 PCT/CN2023/073849 CN2023073849W WO2023143610A3 WO 2023143610 A3 WO2023143610 A3 WO 2023143610A3 CN 2023073849 W CN2023073849 W CN 2023073849W WO 2023143610 A3 WO2023143610 A3 WO 2023143610A3
Authority
WO
WIPO (PCT)
Prior art keywords
fragment
amino acid
apoe
application
acid residue
Prior art date
Application number
PCT/CN2023/073849
Other languages
English (en)
French (fr)
Other versions
WO2023143610A2 (zh
Inventor
陈椰林
侯祥龙
耿泱
张雪馨
Original Assignee
斯莱普泰(上海)生物医药科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 斯莱普泰(上海)生物医药科技有限公司 filed Critical 斯莱普泰(上海)生物医药科技有限公司
Priority to AU2023213923A priority Critical patent/AU2023213923A1/en
Priority to CN202380018990.9A priority patent/CN118786138A/zh
Priority to MX2024009427A priority patent/MX2024009427A/es
Publication of WO2023143610A2 publication Critical patent/WO2023143610A2/zh
Publication of WO2023143610A3 publication Critical patent/WO2023143610A3/zh

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/864—Parvoviral vectors, e.g. parvovirus, densovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Pulmonology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurosurgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Rheumatology (AREA)
  • Psychiatry (AREA)
  • Urology & Nephrology (AREA)
  • Pain & Pain Management (AREA)
  • Hospice & Palliative Care (AREA)
  • Immunology (AREA)

Abstract

本发明公开了载脂蛋白E（ApoE）的C端片段和经修饰的ApoE2以及其在抑制γ-分泌酶酶活中的应用。本发明提供了分离的ApoE构建物，包含一个ApoE多肽，所述ApoE多肽：(1)包含来自SEQ ID NO：12所示氨基酸序列的一个片段，该片段N端第一个氨基酸残基为SEQ ID NO：12第215-221位中任一位置上的氨基酸残基，该片段C端最后一个氨基酸残基为SEQ ID NO：12第274-299位中任一位置上的氨基酸残基；或(2)其氨基酸序列与(1)所述片段具有至少约70％序列同一性，同时保留至少约50％(1)所述片段对γ-分泌酶酶活的抑制。本发明还提供所述ApoE构建物的蛋白部分的编码序列、核酸构建物、宿主细胞、构建方法、药物组合物以及应用。
PCT/CN2023/073849 2022-01-30 2023-01-30 载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用 WO2023143610A2 (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2023213923A AU2023213923A1 (en) 2022-01-30 2023-01-30 C-TERMINAL FRAGMENT OF APOLIPOPROTEIN E AND APPLICATION THEREOF IN INHIBITING γ-SECRETASE ACTIVITY
CN202380018990.9A CN118786138A (zh) 2022-01-30 2023-01-30 载脂蛋白E的C端片段及其在抑制γ分泌酶酶活中的应用
MX2024009427A MX2024009427A (es) 2022-01-30 2023-01-30 Fragmento c-terminal de apolipoproteina e y aplicacion del mismo en la inhibicion de la actividad de \03b3-secretasa.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202210113320.2A CN116554303A (zh) 2022-01-30 2022-01-30 载脂蛋白E的C端片段及其在抑制γ分泌酶酶活中的应用
CN202210113320.2 2022-01-30

Publications (2)

Publication Number Publication Date
WO2023143610A2 WO2023143610A2 (zh) 2023-08-03
WO2023143610A3 true WO2023143610A3 (zh) 2023-10-26

Family

ID=87470769

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2023/073849 WO2023143610A2 (zh) 2022-01-30 2023-01-30 载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用

Country Status (4)

Country Link
CN (2) CN116554303A (zh)
AU (1) AU2023213923A1 (zh)
MX (1) MX2024009427A (zh)
WO (1) WO2023143610A2 (zh)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020147999A1 (en) * 2000-11-03 2002-10-10 Yadong Huang Methods of treating disorders related to apoE
US20050202532A1 (en) * 2002-05-08 2005-09-15 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux
US20100204103A1 (en) * 2002-05-08 2010-08-12 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
AU2353384A (en) 1983-01-19 1984-07-26 Genentech Inc. Amplification in eukaryotic host cells
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020147999A1 (en) * 2000-11-03 2002-10-10 Yadong Huang Methods of treating disorders related to apoE
US20050202532A1 (en) * 2002-05-08 2005-09-15 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux
US20100204103A1 (en) * 2002-05-08 2010-08-12 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHARULATHA VEDHACHALAM ET AL.: "The C-Terminal Lipid-Binding Domain of Apolipoprotein E Is a Highly Efficient Mediator of ABCA1-Dependent Cholesterol Efflux that Promotes the Assembly of High-Density Lipoproteins", BIOCHEMISTRY, vol. 46, no. 10, 17 February 2007 (2007-02-17), pages 2583 - 2593, XP008138940, DOI: 10.1021/bi602407r *
XIONG, HUAQI ET AL.: "Cholesterol Retention in Alzheimer's Brain Is Responsible for High (3- and gamma-secretase Activities and A(3 Production", NEUROBIOL DIS., vol. 29, no. 3, 31 March 2008 (2008-03-31), pages 422 - 437, XP022492237, DOI: 10.1016/j.nbd.2007.10.005 *

Also Published As

Publication number Publication date
AU2023213923A1 (en) 2024-08-22
CN118786138A (zh) 2024-10-15
CN116554303A (zh) 2023-08-08
WO2023143610A2 (zh) 2023-08-03
MX2024009427A (es) 2024-08-14

Similar Documents

Publication Publication Date Title
NZ606427A (en) Extended recombinant polypeptides and compositions comprising same
Wendt et al. Interaction of calpastatin with calpain: a review
Kollmann et al. Identification of novel lysosomal matrix proteins by proteome analysis
Ugai et al. Purification and characterization of the 26S proteasome complex catalyzing ATP-dependent breakdown of ubiquitin-ligated prot from rat liver
Abreu et al. Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity
DE60310264D1 (en) Thermostabile alpha-amylase
Inzitari et al. Detection in human saliva of different statherin and P‐B fragments and derivatives
NZ595499A (en) Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
NZ596764A (en) N-glycan core beta-galactosyltransferase and uses thereof
Sasaki Patchwork‐structure serpins from silkworm (Bombyx mori) larval hemolymph
LaFayette et al. Molecular characterization of cDNAs encoding low-molecular-weight heat shock proteins of soybean
Bolscher et al. A one-enzyme strategy to release an antimicrobial peptide from the LFampin-domain of bovine lactoferrin
WO2010007144A3 (en) Mutated netrin-4 proteins, fragments thereof and their uses as drugs
Su et al. High-level expression and purification of human epidermal growth factor with SUMO fusion in Escherichia coli
MX2022009515A (es) Variantes de alfa-amilasa y polinucleotidos que las codifican.
WO2007142582A8 (en) Fluorescent proteins and genes encoding them
Pérez‐Peinado et al. Structural determinants conferring unusual long life in human serum to rattlesnake‐derived antimicrobial peptide Ctn [15‐34]
Imajoh et al. Calcium-activated neutral protease inhibitor from rabbit erythrocytes lacks the N-terminal region of the liver inhibitor but retains three inhibitory units
Carrell et al. Active site of α1-antitrypsin: Homologous site in antithrombin-III
Chessa et al. Synthetic peptides reproducing the site phosphorylated by cAMP‐dependent protein kinase in protein phosphatase inhibitor‐1: Effect of structural modifications on the phosphorylation efficiency
Dohmae et al. The complete amino acid sequences of two serine proteinase inhibitors from the fruiting bodies of a basidiomycete, Pleurotus ostreatus
WO2023143610A3 (zh) 载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用
IL168793A (en) Streptococcus uberis adhesion molecule
Saitoh et al. Production and characterization of two variants of human cystatin SA encoded by two alleles at theCST2Locus of the type 2 cystatin Gene family
Hartmann et al. Heterodimeric capping proteins constitute a highly conserved group of actin‐binding proteins

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23746487

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: MX/A/2024/009427

Country of ref document: MX

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112024015617

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 2023213923

Country of ref document: AU

Date of ref document: 20230130

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2023746487

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2023746487

Country of ref document: EP

Effective date: 20240830