WO2023143610A3 - 载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用 - Google Patents

载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用 Download PDF

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WO2023143610A3
WO2023143610A3 PCT/CN2023/073849 CN2023073849W WO2023143610A3 WO 2023143610 A3 WO2023143610 A3 WO 2023143610A3 CN 2023073849 W CN2023073849 W CN 2023073849W WO 2023143610 A3 WO2023143610 A3 WO 2023143610A3
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fragment
amino acid
apoe
application
acid residue
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PCT/CN2023/073849
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French (fr)
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WO2023143610A2 (zh
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陈椰林
侯祥龙
耿泱
张雪馨
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斯莱普泰(上海)生物医药科技有限公司
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Priority to AU2023213923A priority Critical patent/AU2023213923A1/en
Priority to MX2024009427A priority patent/MX2024009427A/es
Priority to CN202380018990.9A priority patent/CN118786138A/zh
Publication of WO2023143610A2 publication Critical patent/WO2023143610A2/zh
Publication of WO2023143610A3 publication Critical patent/WO2023143610A3/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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Abstract

本发明公开了载脂蛋白E（ApoE）的C端片段和经修饰的ApoE2以及其在抑制γ-分泌酶酶活中的应用。本发明提供了分离的ApoE构建物，包含一个ApoE多肽，所述ApoE多肽：(1)包含来自SEQ ID NO：12所示氨基酸序列的一个片段，该片段N端第一个氨基酸残基为SEQ ID NO：12第215-221位中任一位置上的氨基酸残基，该片段C端最后一个氨基酸残基为SEQ ID NO：12第274-299位中任一位置上的氨基酸残基；或(2)其氨基酸序列与(1)所述片段具有至少约70％序列同一性，同时保留至少约50％(1)所述片段对γ-分泌酶酶活的抑制。本发明还提供所述ApoE构建物的蛋白部分的编码序列、核酸构建物、宿主细胞、构建方法、药物组合物以及应用。
PCT/CN2023/073849 2022-01-30 2023-01-30 载脂蛋白e的c端片段及其在抑制γ分泌酶酶活中的应用 WO2023143610A2 (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2023213923A AU2023213923A1 (en) 2022-01-30 2023-01-30 C-TERMINAL FRAGMENT OF APOLIPOPROTEIN E AND APPLICATION THEREOF IN INHIBITING γ-SECRETASE ACTIVITY
MX2024009427A MX2024009427A (es) 2022-01-30 2023-01-30 Fragmento c-terminal de apolipoproteina e y aplicacion del mismo en la inhibicion de la actividad de \03b3-secretasa.
CN202380018990.9A CN118786138A (zh) 2022-01-30 2023-01-30 载脂蛋白E的C端片段及其在抑制γ分泌酶酶活中的应用

Applications Claiming Priority (2)

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CN202210113320.2 2022-01-30
CN202210113320.2A CN116554303A (zh) 2022-01-30 2022-01-30 载脂蛋白E的C端片段及其在抑制γ分泌酶酶活中的应用

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WO2023143610A2 WO2023143610A2 (zh) 2023-08-03
WO2023143610A3 true WO2023143610A3 (zh) 2023-10-26

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AU (1) AU2023213923A1 (zh)
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020147999A1 (en) * 2000-11-03 2002-10-10 Yadong Huang Methods of treating disorders related to apoE
US20050202532A1 (en) * 2002-05-08 2005-09-15 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux
US20100204103A1 (en) * 2002-05-08 2010-08-12 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
AU2353384A (en) 1983-01-19 1984-07-26 Genentech Inc. Amplification in eukaryotic host cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020147999A1 (en) * 2000-11-03 2002-10-10 Yadong Huang Methods of treating disorders related to apoE
US20050202532A1 (en) * 2002-05-08 2005-09-15 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux
US20100204103A1 (en) * 2002-05-08 2010-08-12 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHARULATHA VEDHACHALAM ET AL.: "The C-Terminal Lipid-Binding Domain of Apolipoprotein E Is a Highly Efficient Mediator of ABCA1-Dependent Cholesterol Efflux that Promotes the Assembly of High-Density Lipoproteins", BIOCHEMISTRY, vol. 46, no. 10, 17 February 2007 (2007-02-17), pages 2583 - 2593, XP008138940, DOI: 10.1021/bi602407r *
XIONG, HUAQI ET AL.: "Cholesterol Retention in Alzheimer's Brain Is Responsible for High (3- and gamma-secretase Activities and A(3 Production", NEUROBIOL DIS., vol. 29, no. 3, 31 March 2008 (2008-03-31), pages 422 - 437, XP022492237, DOI: 10.1016/j.nbd.2007.10.005 *

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WO2023143610A2 (zh) 2023-08-03
MX2024009427A (es) 2024-08-14
CN118786138A (zh) 2024-10-15
CN116554303A (zh) 2023-08-08
AU2023213923A1 (en) 2024-08-22

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