WO2023137245A1 - Compositions et procédés de lutte contre les nématodes parasitant les plantes - Google Patents

Compositions et procédés de lutte contre les nématodes parasitant les plantes Download PDF

Info

Publication number
WO2023137245A1
WO2023137245A1 PCT/US2023/060202 US2023060202W WO2023137245A1 WO 2023137245 A1 WO2023137245 A1 WO 2023137245A1 US 2023060202 W US2023060202 W US 2023060202W WO 2023137245 A1 WO2023137245 A1 WO 2023137245A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
composition
component
total amount
range
Prior art date
Application number
PCT/US2023/060202
Other languages
English (en)
Inventor
Samuel L. CLOETE
Ahsan Habib
Original Assignee
Kannar Earth Science, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kannar Earth Science, Ltd. filed Critical Kannar Earth Science, Ltd.
Publication of WO2023137245A1 publication Critical patent/WO2023137245A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00—Nematocides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides

Definitions

  • the present disclosure relates to the field of agricultural pest control to reduce crop damage and loss of yield. Specifically, the present disclosure relates to compositions and methods for control of plant pathogens, including parasitic nematodes, in soil or any other growing media where plants are grown.
  • Nematodes are miniscule soil inhabitants that are parasitic to almost all plants on earth. These parasites, commonly known as Plant Parasitic nematodes (PPNs), cause an estimated loss of yields over $ 100 billion annually (Thoden et al., 2011). Most notorious plant nematodes include root knot, cyst, ring, and lesion nematodes. Since these PPNs cannot be eradicated completely, different controlling strategies are practiced reducing their infestation. These strategies include chemical nematicides, agronomic practices (crop rotation, cover crops or biofumigation), biopesticides (fungi, bacteria, or their derivatives) and plant derived formulations (Nicola et al., 2021).
  • the present disclosure is directed to a nematicide composition including: (i) swerdamarin: (ii) berberine; (iii) chitosan; (iv) glutathione: and (v) cellulose- based polymer.
  • a liquid composition including: (i) swertiamarin; (ii) berberine; (iii) chitosan; (iv) glutathione; and (v) cellulose-based polymer, wherein the composition has activity against one or more plant pathogens.
  • the one or more plant pathogens can be one or a combination of a nematode, an insect, or a fungus.
  • composition of the present disclosure can be formulated as a flowable liquid.
  • composition of the invention can be in the form of an emulsion or a suspension.
  • the cellulose-based polymer can include hydroxyethy cellulose or any cellulose-based polymer that keeps the components of the composition in an emulsion or a suspension.
  • the cellulose-based polymer can include hydroxyethylcellulose, hydroxyethyl propyl methyl cellulose (HPMC), quillaja extract, xanthan gum, corn starch, or yucca extract, and combinations thereof.
  • the emulsion or suspension can be an oil-based or an aqueous-based emulsion or suspension.
  • the composition of the invention can have a total amount of component (i) in the range from 0.1 to 1.0 g/L, the total amount of component (ii) in the range from 0.1 to 1.0 g/L, the total amount of component (iii) in the range from 0.1 to 1.0 g/L, and the total amount of component (iv) in the range from 0.1 to 0.5 g/L.
  • the total amount of component (i) in the composition can be equal to or less than 1.0 g/L, the total amount of component (ii) can be equal to or less than 0.5 g/L, the total amount of component (iii) can be equal to or less than 0.5 g/L, and the total amount of component (iv) can be less than or equal to 0.2 g/L.
  • the total amount of component (i) in the composition can be in the range from 0.01 to 1.0 g/L, the total amount of component (ii) can be in the range from 0.005 to 0.5 g/L, the total amount of component (iii) can be in the range from 0.0025 to 0.25 g/L, and the total amount of component (iv) can be in the range from 0.002 to 0.2 g/L.
  • an aqueous composition including a ratio by weight of: (i) about 0.05% to about 0.2% swertiamarin; (ii) about 0.025% to about 0.1% berberine; (iii) about 0.01% to about 0.05% chitosan; (iv) about 0.01% to about 0.04% L-glutathione; and (v) about 80-99% cellulose-based polymer, wherein the composition has activity against one or more plant pathogens.
  • a composition including a ratio by weight of: (i) about 0.1% swertiamarin; (ii) about 0.05% berberine; (iii) about 0.025% chitosan; (iv) about 0.02% L-glutathione; (v) about 2% DMSO; (vi) about 90% hydroxy ethylcellulose carrier; and (vii) about 7.805% water, wherein the composition has activity against one or more plant pathogens.
  • composition of the present disclosure can include one or more plant bio stimulants.
  • the biostimulants can include amino acids, humic acid, fulvic acid, seaweed extract, laminarin, alginates, or polysaccharides, and combinations thereof.
  • the composition of the present disclosure can include one or more antioxidants.
  • the antioxidants can include vitamin C, vitamin E, coenzyme Q10, lipoic acid, flavonoids, phenols, polyphenols, or phytoestrogens, and combinations thereof.
  • the present disclosure is directed to a plant seed coated with a composition including: (i) swertiamarin; (ii) berberine; (iii) chitosan; (iv) glutathione; and (v) cellulose-based polymer, wherein the composition is present in the coating in an amount suitable to improve plant growth and/or health in the presence of a plant pathogen, or both.
  • the present disclosure is direct to a method of plant seed treatment including: applying to seeds of a plant a coating of a liquid composition including: (i) swertiamarin; (ii) berberine; (iii) chitosan; (iv) glutathione; and (v) cellulose-based polymer, wherein the liquid composition is applied to the seeds in the form of a suspension or an emulsion and is present in the coating in an amount suitable to improve plant growth and/or health in the presence of a plant pathogen, or both.
  • a liquid composition including: (i) swertiamarin; (ii) berberine; (iii) chitosan; (iv) glutathione; and (v) cellulose-based polymer, wherein the liquid composition is applied to the seeds in the form of a suspension or an emulsion and is present in the coating in an amount suitable to improve plant growth and/or health in the presence of a plant pathogen, or both.
  • the present disclosure is directed to a method for improving plant growth and/or health including: planting a plant or a seed of a plant in a suitable growth medium, the plant or the seed having a coating or partial coating of a composition including: (i) swertiamarin; (ii) berberine: (iii) chitosan; (iv) glutathione; and (v) cellulose- based polymer, wherein the composition is present in an amount suitable to improve plant growth and/or health in the presence of a plant pathogen, or both.
  • a method for improving plant growth and/or health including: delivering to seed of a plant, foliage of a plant, roots of a plant, or soil or growth medium surrounding a plant, a liquid composition in the form of an emulsion or a suspension including: (i) swertiamarin; (ii) berberine; (iii) L- glutathione; (iv) chitosan; and (v) cellulose-based polymer, wherein the composition improves plant growth and/or health in the presence of a plant pathogen, or both.
  • the composition can exhibit control of one or more of plant parasitic nematodes, fungi, or insects.
  • the composition in which the seeds or plant parts are coated includes: component (i) in the range from 0.1 to 1.0 g/L, component (ii) in the range from 0.5 to 1.0 g/L, component (iii) in the range from 0.1 to 1.0 g/L, and component (iv) in the range from 0.1 to 0.5 g/L.
  • the composition in which the seeds or plant parts are coated includes: component (i) in an amount equal to or less than 1.0 g/L, component (ii) in an amount equal to or less than 0.5 g/L, component (iii) in an amount equal to or less than 0.5 g/L, and component (iv) in an amount equal to or less than 0.2 g/L.
  • the plants or plant seeds can include, but are not limited to, cotton, com, peanut, wheat, soybean, sorghum, or canola, and combinations thereof.
  • the plants or plant seeds include legumes.
  • the plants or plant seeds include non-legumes.
  • Figure 1 is graft showing the effect over time of dilutions of a nematicide composition on mortality of dagger nematodes (Xiphinema sp.).
  • Figure 2 is a graph showing the positive impact on corn seedling growth after seed treatment with a nematicide composition.
  • Figure 3 is a graph showing the effect of a nematicide composition as an in- furrow application in a cotton field trial on reniform nematodes and its impact on improving seed cotton yield.
  • Figure 4A is a graph showing the effect of in-furrow application of a nematicide composition in a soybean field trial on reducing root-knot nematode induced galling.
  • Figure 4B is a graph showing the increased soybean yield in the trial of Figure 4A.
  • Figure 5A is a grayscale image showing the anti-fungal activity in an in vitro petri plate assay of cellulose-based carrier hydroxyethyl cellulose alone against peanut white mold (Sclerotium roljsii).
  • Figure 5B is a grayscale image showing the anti-fungal activity in an in vitro petri plate assay of a IX concentration of nematicide composition against peanut white mold (Sclerotium roljsii).
  • Figure 5C is a grayscale image showing the anti-fungal activity in an in vitro petri plate assay of a 2X concentration of nematicide composition against peanut white mold (Sclerotium rol sii).
  • Figure 5D is a grayscale image showing the anti-fungal activity in an in vitro petri plate assay of commercially available fungicide RANCONA VPD against peanut white mold (Sclerotium roljsii).
  • Figure 6A is a grayscale image showing the anti-fungal activity in an in vitro petri plate assay of a IX concentration of the nematicide composition against peanut black leaf spot (Nothopassalora personata).
  • Figure 6B is a grayscale image showing the anti-fungal activity in an in vitro petri plate assay of a 2X concentration of the nematicide composition against peanut black leaf spot (Nothopassalora personata).
  • Figure 6C is a grayscale image showing the anti-fungal activity in an in vitro petri plate assay of commercially available fungicide RANCONA VPD against peanut black leaf spot (Nothopassalora per sonata) in comparison to (c).
  • the present disclosure provides a nematicide composition that includes extremely bitter plant based saponins, swertiamarin and berberine, chitosan (an EPA approved adjuvant) and an antioxidant for application to plant seeds to kill or deter plant parasitic nematodes.
  • Swertiamarin and berberine are two bitter phytochemicals from Swertia and Berberis plants, respectively.
  • the nematicide composition comprises swertiamarin from Swertia, berberine from Berberis, and chitosan from crustaceans.
  • these components are provided in a liquid solution of a cellulose-based polymer containing an antioxidant such as, for example, glutathione, to protect the active ingredients from possible oxidative degradation.
  • the nematicide composition can reduce the locomotion and kill root-knot nematodes within a few seconds upon contact. This is unexpected given that when swertiamarin, berberine, glutathione, or chitosan are applied individually, nematodes are repelled, but the application is not fatal at the time of application.
  • An example of the killing activity of a water-soluble nematicide composition of the present disclosure is described in Example 1. This composition kills needle nematodes (Longidorus sp.) within a few seconds of contact (see Table 1 for a summary of the results).
  • the nematicide composition includes a ratio by weight of: (i) about 0.1% swertiamarin; (ii) about 0.05% berberine; (iii) about 0.025% water soluble chitosan; (iv) about 0.02% by weight L-glutathione; (v) about 2% by weight of DMSO; and (vi) about 97.805% water.
  • Example 1 Two-minute contact of live needle nematodes (Longidorus sp. with water-based nematicide composition and with each individual component. Additional experiments are described in Example 1 in which the water earner was replaced with a cellulose-based carrier to keep the components in suspension because of precipitation of some components. Specifically, when a cellulose-based polymer was used as a carrier in the composition described in Example 1, water was replaced by 90% cellulose-based liquid polymer and 7.805% water. The liquid cellulose-based composition killed dagger (Xiphinema sp.) nematodes with a 100% killing rate (see Table 2).
  • SWTM swertiamarin
  • BRBN berberine
  • GLTN glutathione
  • CHT chitosan
  • composition including swertiamarin, berberine, chitosan, and glutathione in the cellulose-based polymer described above was stored and kept at room temperature (23 °C) for a month and then tested again on live dagger nematodes.
  • live swimming dagger nematodes Xiphinema sp.
  • the rate of nematode fatality was 100% upon contact with the composition.
  • a summary of the results of the four replicates of this experiment at various dilutions is shown in Figure 1.
  • the total amount of component (i) in the nematicide composition is in the range from 0.01 to 1.00 g/L, the total amount of component (ii) is in the range from 0.005 to 0.5 g/L, the total amount of component (iii) is in the range from 0.0025 to 0.25 g/L, and the total amount of component (iv) is in the range from 0.002 to 0.2
  • the total amount of component (i) in the nematicide composition is in the range from 0.001 to 1.00 g/L, the total amount of component (ii) is in the range from 0.0005 to 0.5 g/L, the total amount of component (iii) is in the range from 0.00025 to 0.25 g/L, and the total amount of component (iv) is in the range from 0.0002 to 0.2
  • the total amount of component (i) in the nematicide composition is equal to or less than 1.0 g/L, the total amount of component (ii) is equal to or less than 0.5 g/L, the total amount of component (iii) is equal to or less than 0.5 g/L, and the total amount of component (iv) is equal to or less than 0.2 g/L.
  • Example 2 of the present disclosure describes an experiment in which treatment of com seeds with the nematicide composition results in enhanced seedling growth.
  • seedling roots and shoots were measured after treated corn seeds were placed on wet germination paper and kept in the dark for seven days. There was no significant difference in seedling main root length but the nematicide composition significantly increased (24%) coleoptile length (see Figure 2).
  • the present disclosure is directed to a seed coated with a composition including: (i) swertiamarin; (ii) berberine; (iii) chitosan; (iv) glutathione; and (v) cellulose-based polymer, wherein the composition is present in the coating in an amount suitable to improve plant growth and/or health in the presence of a plant pathogenic nematode, or both.
  • the present disclosure is direct to a method of plant seed treatment including applying to seeds of a plant a coating of a composition including: (i) swertiamarin; (ii) berberine; (iii) chitosan; (iv) glutathione and, (v) cellulose-based polymer, wherein the composition is present in the coating in an amount suitable to improve plant growth and/or health in the presence of a plant pathogenic nematode, or both.
  • Examples 3 and 4 of the present disclosure describe field performance of the nematicide composition.
  • composition applied in furrow in cotton and soybean field trials reduced reniform nematodes in cotton soil and root knot nematode induced root galling in soybean and improved cotton and soybean yields. See Figure 3 for the cotton field trial results and Figures 4 A and 4B for the soybean field trial results.
  • Example 5 of the present disclosure describes an experiment in which the current composition exhibited anti-fungal activity through in vitro assays.
  • the composition was able to show some degree of control of peanut white mold (Sclerotium rolfsii) and black leaf spot fungi (Nothopassarola per sonata).
  • peanut white mold Sclerotium rolfsii
  • black leaf spot fungi Nothopassarola per sonata
  • plates treated with cellulose-based carrier hydroxy ethyl cellulose alone did not exhibit any control of fungus (see Fig. 5a).
  • Fungicide RANCONA VPD showed some control of fungus by zone of inhibition (or zone of clearance) but soon got occupied by fungus (see Fig. 5d).
  • the nematicide composition both at lx (see Fig.
  • fungicide RANCONA VPD exhibited good control of fungus by producing larger clearing zone around the point of application (see Fig. 6c).
  • the nematicide composition at lx and 2x concentrations also exhibited control of fungal growth but the effect was less than fungicide RANCONA VPD (Fig. 6a & b).
  • the nematicide composition showed a similar pattern of control efficacy at 1 x and 2x concentrations.
  • Example 6 of the present disclosure describes an experiment in which the composition also exhibited insecticidal activity in laboratory assays.
  • Plant feeding insects particularly those under the coleoptera order (most beetles and weevils) are very voracious insect for crops.
  • the composition when applied directly to the body of the coleoptera insect larvae demonstrated a high degree (100%) of paralysis followed by death to the larvae (data not shown).
  • the present disclosure is directed to a method for improving plant growth and/or health including: planting a plant or a seed of a plant in a suitable growth medium, the plant or the seed having a coating or partial coating of a composition including: (i) swertiamarin; (ii) berberine; (iii) chitosan; (iv) glutathione; and (v) cellulose-based polymer, wherein the composition is present in an amount suitable to improve plant growth and/or health in the presence of a plant pathogen, or both.
  • a method for improving plant growth and/or health including: delivering to seed of a plant, foliage of a plant, roots of a plant, or soil or growth medium surrounding a plant, a liquid composition in the form of an emulsion or a suspension including: (i) swertiamarin; (ii) berberine; (iii) L- glutathione; (iv) chitosan; and (v) cellulose-based polymer, wherein the composition improves plant growth and/or health in the presence of a plant pathogen, or both.
  • the composition can exhibit control of one or more of plant parasitic nematodes, fungi, or insects.
  • the plants or plant seeds can include, but are not limited to, cotton, com, peanut, wheat, soybean, sorghum, or canola, and combinations thereof.
  • the plants or plant seeds include legumes. In other embodiments, the plants or plant seeds include non-legumes.
  • the term “about” when used in connection with one or more numbers or numerical ranges should be understood to refer to all such numbers, including all numbers in a range and modifies that range by extending the boundaries above and below the numerical values set forth.
  • the recitation of numerical ranges by endpoints includes all numbers, e.g., whole integers, including fractions thereof, subsumed within that range (for example, the recitation of 1 to 5 includes 1, 2, 3, 4, and 5, as well as fractions thereof, e.g., 1.5, 2.25, 3.75, 4.1, and the like) and any range within that range.
  • the term "about”, when referring to a value can encompass variations of, in some embodiments +/-100%, in some embodiments +/-50%, in some embodiments +/-20%, in some embodiments +/-10%, in some embodiments +/- 5%, in some embodiments +/-1%, in some embodiments +/-0.5%, and in some embodiments +/-0.1 % , from the specified amount, as such variations are appropriate in the disclosed compositions and methods.
  • the term “about” meaning within an acceptable error range for the particular value should be assumed.
  • Live root knot nematodes were collected from soil and kept in shallow water in small glass beakers at room temperature.
  • the individual components and combined composition were prepared according to the following procedures.
  • Stock solutions of individual components were prepared in water at the concentrations as follows: 1000 ppm swertiamarin (95% purity; Xi’an Best Biotech Co, China), 500 ppm berberine HC1 (98% purity; Shaanxi Jintai Biological Engineering Co Ltd, China), 250 ppm water soluble chitosan (85% purity; Shaanxi Rainwood Biotech Co Ltd, China) and 200 ppm L-glutathione (99% purity; Hunan Insen Biotech Co Ltd, China).
  • Swertiamarin and berberine were dissolved in 0.5 and 1.0 ml of DMSO separately (total 1.5 ml DMSO) prior to dilution in water. The pH of the composition was adjusted to 5.7.
  • the combined composition included: (i) about 0.1% swertiamarin; (ii) about 0.05% berberine; (iii) about 0.025% water soluble chitosan; (iv) about 0.02% by weight L- glutathione; (v) about 2% by weight of DMSO. This is the composition used in the experiment described below.
  • Each of the four agents swertiamarin, berberine, glutathione, and chitosan when applied alone using the water-based composition exhibited reactions by the needle nematodes via faster locomotion and swimming away from the point of application in the measured 1-2 minutes period but did not kill the nematodes. This reaction is a repellent effect.
  • Swertiamarin and berberine were dissolved in 0.5 and 1.0 ml of DMSO separately (total 1.5 ml DMSO) prior to addition to the carrier.
  • Chitosan and glutathione were dissolved in 7.8 ml water prior to addition to the 90.0 ml carrier.
  • the pH of the composition was adjusted to 5.7.
  • the combined composition included: (i) about 0.1% swertiamarin; (ii) about 0.05% berberine; (iii) about 0.025% water soluble chitosan; (iv) about 0.02% by weight L-glutathione; (v) about 2% by weight of DMSO; (vi) about 90% cellulose-based liquid polymer; and (vii) about 7.805% by weight water.
  • the nematicide composition in cellulose-based polymer described above was stored and kept at room temperature (23 °C) for a month and then tested again on live dagger nematodes (Xiphinema .sp.). In the test, there were four replicates each containing a live dagger nematode swimming in water in circular glass plates. A drop or two of the composition was added to the swimming nematodes, which instantly (30-60 seconds) killed all the nematodes. The rate of nematode fatality was 100% upon contact that occurred from 0.5 min to 1 hour depending on various dilutions of the nematicide concentrate (ready-to-use) composition. A summary of the results of the experiment is shown in Figure 1. The composition repeatedly killed dagger nematodes at time of contact (within 1 minute).
  • Example 2 Composition Applied as a Seed Treatment Improves Plant Growth
  • Hybrid com seeds (UPL variety, 2020) were treated with nematicide composition or water as a control at the rate of 25 fl oz/100 lbs of seeds.
  • Hybrid com seeds For a small amount, about 100-gram corn seeds were treated with 1.63 ml of composition in a ZIPLOC bag and then were shaken vigorously for 1 minutes to have a good seed coating.
  • Treated seeds were placed on wet germination paper in plastic trays and kept in the dark for seven days. Seedling roots and shoots (coleoptiles) were measured using WINRHIZO root scanner (Regent Instrument, Canada).
  • Figure 2 shows the positive impact on com seedling growth after seed treatment with nematicide composition. There was no negative impact on root growth while a significant enhancement (@ 0.05 level) of com seedling shoot (coleoptile) growth was observed.
  • the nematicide composition reduced the number of reniform nematodes (Rotylenchulus .sp.) by 26.37% and increased seed cotton production by 348 Ibs/A, equal to 14% increase as compared to control ( Figure 3).
  • Example 5 Composition Exhibits Control of Plant Parasitic Fungi
  • the nematicide composition was also tested against plant pathogenic fungi.
  • White mold Sclerotium rolfsii
  • black leaf spot fungi Nothopassarola personata
  • nematicide composition was tested at two concentrations lx and 2x, against the growth of fungal spores on culture medium.
  • An industry standard fungicide, RANCONA VPD from UPL
  • the white mold assay is shown in Figure 5 and the black leaf spot fungi assay in Figure 6.
  • the cellulose- based carrier hydroxyethyl cellulose alone did not exhibit any control of fungus (Fig.
  • Fungicide VPD showed some control of fungus by zone of inhibition (or zone of clearance) but soon got occupied by fungus (Fig. 5d).
  • the nematicide composition both at lx (Fig. 5b) and 2x (Fig. 5c) concentration restricted fungal growth around the application areas at the center of the culture plates.
  • fungicide VPD showed a good control of fungus by producing larger clearing zone around the point of application (Fig. 6c).
  • the nematicide composition at lx and 2x concentrations also exhibited control of fungal growth but the effect was less than fungicide VPD (Fig. 6a & b).
  • the nematicide composition showed a similar pattern of control efficacy at 1 x and 2x concentrations. These zone of inhibition assays demonstrate the antifungal activity of the composition and its broad- spectrum nature.
  • the nematicide composition was also tested against insects. Plant feeding insects, particularly those under the coleoptera order (most beetles and weevils) are very voracious insect for crops. As surrogates of coleoptera insects’ larvae rootworm and wireworm, Superworm (Zophobas mor io) and mealworm (Tenebrio molitor) were used in this experiment. Individual live insect larva was placed in a glass beaker or a plastic weighing tray, and about one milliliter of the nematicide composition was applied onto the body of a larva. At contact, the larva reacted by shaking its body and turning sideway or upside, and then it became paralyzed within 2 minutes and eventually died within 15-20 minutes (data not shown). These results demonstrate the insecticidal effect of the composition as a broad spectrum biopesticide.
  • compositions and methods have been described in reference to specific embodiments, features, and illustrative embodiments, it will be appreciated that the utility of the subject matter is not thus limited, but rather extends to and encompasses numerous other variations, modifications and alternative embodiments, as will suggest themselves to those of ordinary skill in the field of the present subject matter, based on the disclosure herein.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Insects & Arthropods (AREA)
  • Botany (AREA)
  • Agronomy & Crop Science (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

Une composition nématicide comprend : (i) de la swertiamarine; (ii) de la berbérine; (iii) du chitosane, (iv) du glutathion; et (v) un polymère à base de cellulose. La composition peut tuer des nématodes de la nodosité des racines en une minute de contact. Ce résultat est surprenant, étant donné que chacun de la swertiamarine, de la berbérine, du chitosane et du glutathion, lorsqu'appliqué individuellement, repousse, mais ne tue pas les nématodes. De plus, la longueur de coléoptile résultant du traitement de la graine de maïs avec la composition est grandement augmentée. En outre, la composition appliquée en sillon dans des champs de coton et de soja entraîne une réduction des nématodes réniformes et de la nodosité des racines, respectivement, ainsi qu'un rendement accru. La composition nématicide peut également présenter des activités antifongiques et insecticides.
PCT/US2023/060202 2022-01-11 2023-01-06 Compositions et procédés de lutte contre les nématodes parasitant les plantes WO2023137245A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263298257P 2022-01-11 2022-01-11
US63/298,257 2022-01-11

Publications (1)

Publication Number Publication Date
WO2023137245A1 true WO2023137245A1 (fr) 2023-07-20

Family

ID=87279754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/060202 WO2023137245A1 (fr) 2022-01-11 2023-01-06 Compositions et procédés de lutte contre les nématodes parasitant les plantes

Country Status (2)

Country Link
AR (1) AR128252A1 (fr)
WO (1) WO2023137245A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9113636B2 (en) * 2013-06-26 2015-08-25 Symbiota, Inc. Seed-origin endophyte populations, compositions, and methods of use
US20160128944A1 (en) * 2013-06-04 2016-05-12 Vyome Biosciences Pvt. Ltd. Coated particles and compositions comprising same
US20170087199A1 (en) * 2016-02-10 2017-03-30 Senomyx, Inc. Compositions for delivering a cooling sensation
US20180020677A1 (en) * 2014-12-30 2018-01-25 Indigo Agriculture, Inc. Seed endophytes across cultivars and species, associated compositions, and methods of use thereof
US20190039964A1 (en) * 2017-01-12 2019-02-07 Pivot Bio, Inc. Methods and compositions for improving plant traits
US20190059390A1 (en) * 2015-06-08 2019-02-28 Indigo Ag, Inc. Streptomyces endophyte compositions and methods for improved agronomic traits in plants

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160128944A1 (en) * 2013-06-04 2016-05-12 Vyome Biosciences Pvt. Ltd. Coated particles and compositions comprising same
US9113636B2 (en) * 2013-06-26 2015-08-25 Symbiota, Inc. Seed-origin endophyte populations, compositions, and methods of use
US20180020677A1 (en) * 2014-12-30 2018-01-25 Indigo Agriculture, Inc. Seed endophytes across cultivars and species, associated compositions, and methods of use thereof
US20190059390A1 (en) * 2015-06-08 2019-02-28 Indigo Ag, Inc. Streptomyces endophyte compositions and methods for improved agronomic traits in plants
US20170087199A1 (en) * 2016-02-10 2017-03-30 Senomyx, Inc. Compositions for delivering a cooling sensation
US20190039964A1 (en) * 2017-01-12 2019-02-07 Pivot Bio, Inc. Methods and compositions for improving plant traits

Also Published As

Publication number Publication date
AR128252A1 (es) 2024-04-10

Similar Documents

Publication Publication Date Title
Pavela Possibilities of botanical insecticide exploitation in plant protection
Mukhtar et al. Nematicidal activities of Cannabis sativa L. and Zanthoxylum alatum Roxb. against Meloidogyne incognita
Khan et al. Management of the root-knot nematode Meloidogyne graminicola infesting rice in the nursery and crop field by integrating seed priming and soil application treatments of pesticides
Rioba et al. Ageratum conyzoides L. for the management of pests and diseases by small holder farmers
Rahman et al. Induction of systemic resistance in cotton by the neem cake and Pseudomonas aeruginosa under salinity stress and Macrophomina phaseolina infection
Kong Ecological pest management and control by using allelopathic weeds (Ageratum conyzoides, Ambrosia trifida, and Lantana camara) and their allelochemicals in China
Shafique et al. Role of antagonistic microorganisms and organic amendment in stimulating the defense system of okra against root rotting fungi
Prakash et al. Botanical pesticides for the management of plant nematode and mite pests
Nile et al. Nematicidal potential and specific enzyme activity enhancement potential of neem (Azadirachta indica A. Juss.) aerial parts
Bakshi et al. Marigold biopesticide as an alternative to conventional chemical pesticides
Tuncsoy Nematicidal activity of silver nanomaterials against plant-parasitic nematodes
AU2008225257A1 (en) Pesticidal plant extract containing loline derivatives
WO2015185994A1 (fr) Compositions anti-microbiennes, préparations, méthodes, et utilisations
KR20190050048A (ko) 알피니아 갈랑가 추출물, 계피 추출물, 및 님 종자 추출물을 유효성분으로 함유하는 선충 방제용 조성물
Prasad et al. Management of plant parasitic nematodes by the use of botanicals
WO2023137245A1 (fr) Compositions et procédés de lutte contre les nématodes parasitant les plantes
Tripathi et al. Antifungal activity of Capparis decidua extracts against seed borne pathogenic fungi
EP3357340B1 (fr) Une composition désinfectante écologique des sols agricoles comprenant un mélange d'extrait de capsicum, d'extrait de souci et d'extrait d'ail
Gahukar Use of indigenous plant products for management of pests and diseases of spices and condiments: Indian perspective
Benit et al. Phytopathogenic bacterial and nematicidal activity of extracts and powder of Adhatoda vasica on Meloidogyne incognita
JP2023538601A (ja) アフェレンコイデス属の植物寄生性線虫、特にイネシンガレセンチュウによる植物の被害を防除又は防止する方法
El-Shennawy et al. Management of wilt disease complex caused by Meloidogyne javanica and Fusarium oxysporum f. sp. lycopersici on tomato using some plant extracts
US20240041039A1 (en) Compositions and methods for controlling plant pathogens including nematodes
Khanal et al. Efficacy of entomo-pathogenic fungus and botanical pesticides against mustard aphid (Lipaphis erysimi Kalt.) at field condition Rupandehi Nepal
Prasannakumar et al. Evaluation of some leaf and seed extracts for their insecticidal properties against Aphis gossypii Glover (hemiptera: Aphididae)

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23740744

Country of ref document: EP

Kind code of ref document: A1