WO2023121321A1 - Use of asaroidoxazine b having anticancer activity - Google Patents

Use of asaroidoxazine b having anticancer activity Download PDF

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WO2023121321A1
WO2023121321A1 PCT/KR2022/021008 KR2022021008W WO2023121321A1 WO 2023121321 A1 WO2023121321 A1 WO 2023121321A1 KR 2022021008 W KR2022021008 W KR 2022021008W WO 2023121321 A1 WO2023121321 A1 WO 2023121321A1
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asaroidoxazine
cancer
isomer
formula
preventing
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PCT/KR2022/021008
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French (fr)
Korean (ko)
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이준
서영혜
문병철
이아영
류승목
김효선
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한국한의학연구원
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Publication of WO2023121321A1 publication Critical patent/WO2023121321A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5365—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines ortho- or peri-condensed with heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/26—Aristolochiaceae (Birthwort family), e.g. heartleaf
    • A61K36/268—Asarum (wild ginger)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D498/04—Ortho-condensed systems

Definitions

  • the present invention relates to the use of asaroidoxazine B having anticancer activity, and more particularly, to a pharmaceutical composition using asaroidoxazine B represented by the following formula (1), an isomer or a pharmaceutically acceptable salt thereof, and health functional food composition; a method for preventing, improving or treating cancer using the same; and a method for separating the asaroidoxazine B.
  • Sesin Asarum heterotropoides Fr.Schmidt var. mandshuricum (Maxim.) Kitag.
  • the roots and rhizomes of A. heterotropoides have been used in traditional medicine in some Asian countries to treat cough, headache, toothache, aphthous stomatitis, inflammation, local anesthesia and rheumatism [R. Zhou, "Resource science of Chinese medicinal materials", China Medical and Pharmaceutical Sciences Press, Beijing (1993); J. Lee et al., "Bioactive compounds from the roots of Asiasarum heterotropoides", Molecules 19 (1) (2014) 122-138].
  • the inventors of the present invention completed the present invention by isolating an asaroidoxazine B compound from the roots of sesame seeds and confirming its inhibitory effect on human cancer cell lines.
  • an object of the present invention is to provide an anti-cancer composition using a compound isolated from the secinum root.
  • another object of the present invention is to provide a method for preventing, ameliorating or treating cancer using a compound isolated from the root of Sesin.
  • another object of the present invention is to provide the use of a compound isolated from the root of Sesin in the preparation of a pharmaceutical or health functional food for the prevention, improvement or treatment of cancer.
  • Another object of the present invention is to provide a method for isolating a compound having anti-cancer activity from secin root.
  • the present invention provides a pharmaceutical composition for preventing or treating cancer comprising Asaroidoxazine B represented by Formula 1 below, an isomer thereof or a pharmaceutically acceptable salt thereof as an active ingredient. do:
  • the present invention provides a health functional food composition for preventing or improving cancer comprising the aforementioned compound, its isomer or food-acceptable salt as an active ingredient.
  • the present invention further provides for the prevention of cancer, comprising administering Asaroidoxazine B represented by Formula 1, an isomer or a pharmaceutically acceptable salt thereof to a subject in need thereof. , improvement or treatment methods are provided.
  • the present invention further relates to asaroidoxazine B represented by Formula 1, an isomer thereof, or a pharmaceutical or food product thereof when preparing a pharmaceutical or health functional food for the prevention, improvement or treatment of cancer.
  • asaroidoxazine B represented by Formula 1 an isomer thereof, or a pharmaceutical or food product thereof when preparing a pharmaceutical or health functional food for the prevention, improvement or treatment of cancer.
  • the isomers may include racemates, enantiomers, diastereomers, mixtures of enantiomers or mixtures of diastereomers.
  • the Asaroidoxazine B may be isolated from the roots of Asarum heterotropoides .
  • the asaroidoxazine B, an isomer thereof, or a pharmaceutically or food-acceptable salt thereof may exhibit an effect of inhibiting cancer cells or inhibiting or delaying tumor development.
  • the present invention also provides a method for isolating Asaroidoxazine B represented by Formula 1 from Asarum heterotropoides roots, comprising the following steps:
  • the ethanol solvent in step (a) may be 50 to 90% ethanol solvent.
  • the water-methanol mixture in step (c) may be applied with a concentration gradient of 6:4 to 1:9.
  • the water-acetonitrile mixture of step (d) may be applied with a concentration gradient of 6.5:3.5 to 5.5:4.5.
  • Asaroidoxazine B provided in the present invention exhibits excellent anticancer activity even at a significantly lower concentration than 5-FU, an existing anticancer drug, and can be effectively used for preventing, improving or treating cancer, and preventing side effects due to excessive use can do.
  • Figure 1 shows the chemical structure of compound 1 (asaroidoxazine B) isolated from secin.
  • Figure 2 shows an X-ray ORTEP diagram of compound 1 (asaroidoxazine B).
  • Figure 3 shows the computational and experimental ECD spectra of compound 1 (asaroidoxazine B).
  • Figures 4a to 4f show the results of measuring the cell proliferation inhibitory effect of compound 1 (asaroidoxazin B) on various cancer cell lines by MTT assay
  • Figure 4a is human melanoma cells
  • Figure 4b is non-melanoma skin cancer cells
  • FIG. 4C is human prostate cancer cells
  • FIG. 4D is human lung cancer cells
  • FIG. 4E is human colon cancer cells
  • FIG. 4F is human gastric cancer cells
  • FIG. 4G is human breast adenocarcinoma cells
  • FIG. 4H is human leukemia cells
  • FIG. 4I is Human brain glioblastoma cells
  • Fig. 4j shows the cytostatic effect on human renal adenocarcinoma cells
  • Fig. 4k shows clear cell renal carcinoma.
  • the present inventors isolated the asaroidoxazine B compound from the roots of sesame seeds and confirmed its excellent inhibitory effect on human cancer cell lines.
  • a first aspect of the present invention is a pharmaceutical composition for preventing or treating cancer comprising asaroidoxazine B represented by Formula 1 below, an isomer thereof or a pharmaceutically acceptable salt thereof as an active ingredient; And asaroidoxazine B represented by the following formula (1), an isomer thereof, or a food-acceptable salt thereof as an active ingredient. It relates to a health functional food composition for preventing or improving cancer:
  • the body as defined in the present invention may be Asarum heterotropoides Fr. mandshuricum, ethnic sieboldi Miq, or Asarum maculatum, preferably Asarum heterotropoides Fr. mandshuricum. .
  • the isomer may include, but is not limited to, a racemate, an enantiomer, a diastereomer, a mixture of enantiomers, or a mixture of diastereomers.
  • the asaroidoxazine B may be isolated from Asarum heterotropoides , preferably from roots.
  • the body wash can be purchased commercially, or used collected or cultivated in nature.
  • Such cancers include, for example, melanoma, non-melanoma skin cancer (eg, squamous cell carcinoma), breast cancer, head and neck cancer, thyroid cancer, fibrosarcoma, soft tissue sarcoma, osteosarcoma, testicular cancer, prostate cancer, ovarian cancer, Bladder cancer, skin cancer, brain cancer, glioma, hemangiosarcoma, mastocytoma, leukemia, lymphoma, liver cancer, lung cancer, pancreatic cancer, stomach cancer, kidney cancer, colorectal cancer, hematopoietic tumor, neuroblastoma, epidermal carcinoma, or metastasis thereof, but is limited thereto. It doesn't work.
  • asaroidoxazine B was isolated from the roots of sesame seeds and its anticancer activity was confirmed.
  • asaroidoxazine B is effective against melanoma, non-melanoma skin cancer, prostate cancer, lung cancer, colon cancer, gastric cancer, breast cancer, leukemia, glioma and renal cancer. showed antiproliferative activity.
  • it was found to have significantly superior anticancer activity compared to the positive control 5-FU, which exhibits a cell proliferation inhibitory effect even at the nanomolar (nM) level and exhibits an effect at the micromolar ( ⁇ M) level.
  • the anticancer activity of asaroidoxazine B isolated from sesame root was evaluated in an epidermal growth factor (EGF)-induced carcinogenesis model.
  • EGF epidermal growth factor
  • asaroidoxazine B showed an inhibition rate of 31.1 to 73.4% compared to the EGF-treated group in the EGF-induced carcinogenesis model, and the inhibition rate was increased in a concentration-dependent manner of asaroidoxazine B.
  • the sessile root extract or fraction containing it can also be used for preventing, improving or treating cancer.
  • extract refers to an extract obtained by the extraction treatment of the root of the shoot, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a purified product or a purified product of the extract, or a mixture thereof. Etc., the extract itself and extracts of all formulations that can be formed using the extract.
  • the extract may be extracted from a natural, hybrid or mutant plant of seshin, and may also be extracted from plant tissue culture.
  • the extract may be prepared by using hexane, chloroform, methylene chloride, ethyl acetate, acetone, C 1 to C 4 lower alcohol, water or a mixed solvent thereof as an extraction solvent, preferably C 1 to C 4 It may be prepared by using a lower alcohol, water or a mixed solvent thereof as an extraction solvent, but is not limited thereto.
  • the extract of the extract may be prepared by extracting the dried roots of the shoot at least once using the extraction solvent, and the solvent extract is concentrated under reduced pressure and then prepared as a dry extract obtained by freeze-drying or spray drying. can do.
  • fraction refers to a result obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
  • the sessile root fraction containing asaroidoxazine B may be obtained by (a) extracting the sessile root with an ethanol solvent to obtain an ethanol extract of the sessile root; And (b) it can be obtained by carrying out the step of obtaining an ethyl acetate fraction by systematically fractionating the secinum root ethanol extract in the order of hexane, ethyl acetate and water, but is not limited thereto.
  • the ethanol solvent may be 50 to 90% ethanol, preferably 60 to 80% ethanol, and most preferably 65 to 75% ethanol. In the case of using a solvent other than ethanol, the content of the asaroidoxazine B is rapidly reduced, and the intended anticancer activity may not be exhibited.
  • prevention means any action to suppress or delay the onset of a disease or condition. In the present invention, it means delaying the onset of cancer or suppressing the onset of cancer.
  • the term "improvement” means any action that improves or beneficially changes a disease or condition, and in the present invention, it means improving the symptoms of cancer.
  • treatment means any action that delays, stops, or reverses the progression of a disease or condition, and in the present invention, it means reducing, alleviating, eliminating, or reversing the symptoms of cancer. do.
  • the present invention further relates to the prevention, improvement or prevention of cancer, comprising administering asaroidoxazine B, an isomer or a pharmaceutically acceptable salt thereof represented by Formula 1 above to a subject in need thereof. It is about treatment methods.
  • the present invention further relates to the use of asaroidoxazine B represented by Formula 1, an isomer thereof, or a pharmaceutically or foodtically acceptable salt thereof in the preparation of a pharmaceutical or health functional food for the prevention, improvement or treatment of cancer. It's about use.
  • the term "pharmaceutically acceptable salt” refers to a concentration that has an effective effect that is relatively non-toxic and harmless to patients, and the side effects caused by the salt do not reduce the beneficial efficacy of the compound of Formula 1.
  • inorganic and organic acids can be used as free acids, hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, perchloric acid, and phosphoric acid can be used as inorganic acids, and citric acid, acetic acid, lactic acid, maleic acid, and fumarin as organic acids.
  • Acids gluconic acid, methanesulfonic acid, glycolic acid, succinic acid, tartaric acid, galacturonic acid, embonic acid, glutamic acid, aspartic acid, oxalic acid, (D) or (L) malic acid, maleic acid, methanesulfonic acid, ethanesul
  • phonic acid, 4-toluenesulfonic acid, salicylic acid, citric acid, benzoic acid or malonic acid may be used.
  • these salts include alkali metal salts (sodium salt, potassium salt, etc.) and alkaline earth metal salts (calcium salt, magnesium salt, etc.) and the like.
  • acid addition salts include acetate, aspartate, benzate, besylate, bicarbonate/carbonate, bisulfate/sulfate, borate, camsylate, citrate, edisylate, esylate, formate, fumarate, Gluceptate, gluconate, glucuronate, hexafluorophosphate, hybenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, malic Eight, malonate, mesylate, methyl sulfate, naphthylate, 2-naphsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, saccharide Lates, stearates, succinates, tartrates, tosylates,
  • the pharmaceutical composition of the present invention may additionally include a pharmaceutically acceptable carrier.
  • the pharmaceutical composition of the present invention can be prepared into a pharmaceutical formulation using methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal.
  • preparation of a dosage form it is preferable to mix or dilute the active ingredient with a carrier or to encapsulate it in a carrier in the form of a container.
  • the pharmaceutical composition of the present invention is formulated according to conventional methods into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and sterile injection solutions. It may further include appropriate carriers, excipients and diluents commonly used in the preparation of the composition.
  • carriers that may be included in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants.
  • Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, for example, starch, calcium carbonate, sucrose, etc. ) or by mixing lactose and gelatin.
  • excipients for example, starch, calcium carbonate, sucrose, etc.
  • lubricants such as magnesium stearate and talc are also used.
  • Liquid preparations for oral use include suspensions, solutions for oral use, emulsions, syrups, etc., and various excipients such as wetting agents, sweeteners, aromatics, preservatives, etc. may be included in addition to water and liquid paraffin, which are commonly used simple diluents. there is.
  • Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories.
  • Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents.
  • injectable esters such as ethyl oleate
  • witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogeratin and the like may be used as a base for the suppository.
  • the term "administration" means introducing the pharmaceutical composition of the present invention to a patient by any suitable method.
  • the administration method of the pharmaceutical composition according to the present invention is not particularly limited, and may follow a method commonly used in the art.
  • the administration method is not limited as long as it can reach the target tissue, but may be intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, or intranasal administration.
  • the pharmaceutical composition according to the present invention may be prepared in various formulations depending on the desired administration method.
  • the pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount.
  • the "pharmaceutically effective amount” means an amount sufficient to treat a disease with a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is subject type and severity, age, sex, infected virus type, drug activity, drug sensitivity, time of administration, route of administration and excretion rate, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field.
  • a typical daily dose of the pharmaceutical composition according to the present invention can be appropriately selected by those skilled in the art, and can be administered once or divided into several times.
  • composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into 2 to 3 times.
  • composition of the present invention can be used alone or in combination with other drug treatments for the prevention or treatment of cancer. It is important to administer the amount that can obtain the maximum effect with the minimum amount without side effects in consideration of all the above factors, and can be easily determined by those skilled in the art.
  • the term "subject” refers to all animals, including humans, who have or are likely to develop cancer.
  • the animal may be not only humans but also mammals such as cattle, horses, sheep, pigs, goats, camels, antelopes, dogs, and cats that require treatment for similar symptoms, but are not limited thereto.
  • the term "food” refers to meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcohol Beverages, vitamin complexes, health functional foods and health foods, etc., include all foods in a conventional sense.
  • the health functional food composition of the present invention includes forms such as pills, powders, granules, precipitates, tablets, capsules or liquids, and as foods to which the composition of the present invention can be added, for example, various foods, such as For example, there are beverages, chewing gum, tea, vitamin complexes, and health supplements.
  • the food auxiliary additives include food auxiliary additives common in the art, such as flavoring agents, flavoring agents, coloring agents, fillers, stabilizers, and the like.
  • Examples of the natural carbohydrate include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol.
  • natural flavors eg, rebaudioside A, glycyrrhizin, etc.
  • synthetic flavors sacharin, aspartame, etc.
  • the health functional food composition of the present invention is various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like.
  • it may contain fruit flesh for the manufacture of natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination.
  • the health supplement includes health functional food and health food.
  • the functional food is the same term as food for special health use (FoSHU), and is a medicine processed to efficiently display bioregulatory functions in addition to nutrient supply, and has high medical effect.
  • “function (sex)” means to obtain useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions.
  • the food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and ingredients commonly added in the art during the preparation.
  • the formulation of the food may also be prepared without limitation as long as the formulation is recognized as a food.
  • the health functional food composition of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time using food as a raw material.
  • the term "subject” includes any animal (eg, human, horse, pig, rabbit, dog, sheep, goat, non-human primate, cow, cat, guinea pig, or rodent), but It is not limited to this. These terms do not denote a specific age or gender. Thus, adult/adult and newborn subjects, whether female/female or male/male, as well as fetuses are intended to be included.
  • a patient refers to a subject suffering from a disease or disorder. The term patient includes human and veterinary subjects.
  • asaroidoxazine B represented by Formula 1 its isomer, its pharmaceutically or food-acceptable salt, or the effect of the root fraction and its administration route, including the number of administrations, dosage, etc. Since the description of the configuration is the same as described above, the description thereof is omitted.
  • a second aspect of the present invention relates to a method for isolating Asaroidoxazine B represented by Formula 1 from Asarum heterotropoides roots, comprising the following steps:
  • the thin roots of step (a) may be dried through a conventional drying method such as natural drying or hot air drying method.
  • the type of extraction solvent used to extract the roots of the shoot is ethanol.
  • Ethanol extraction i.e., alcohol extraction
  • ethanol extraction is the most eco-friendly and economical extraction method in food processing.
  • 50% to 90% ethanol, more preferably 60% to 80% ethanol, and most preferably 65% to 75% ethanol may be used as the extraction solvent.
  • a method for preparing the extract is not particularly limited, and may be extracted according to a method commonly used in the art.
  • Non-limiting examples of the extraction method include immersion extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, and the like, which may be performed alone or in combination of two or more methods.
  • step (c) may be to perform flash chromatography by applying a water-methanol mixture at a concentration gradient of 6:4 to 1:9, preferably a standard reversed-phase hydrophobic resin, For example, it can be performed by flash chromatography using a column packed with C18 resin.
  • the water-acetonitrile mixture in step (d) may be applied with a concentration gradient of 6.5:3.5 to 5.5:4.5 to perform semi-preparative chromatography, preferably a standard reversed-phase hydrophobic resin. , for example, by semipreparative chromatography using a column packed with ODS-A resin.
  • Optical rotation and UV spectra were determined using an MCP-5500 digital polarimeter (Anton Paar, Graz, Austria) and an Optizen POP spectrophotometer (Mecasys, Daejeon, Korea), respectively.
  • ECD Electronic circular dichroism
  • NMR spectra were acquired on an AVANCE III 600 MHz Cryo-NMR (Bruker, Billerica, MA, USA).
  • HRESIMS data were acquired using a Bruker compact or Waters Q-TOF mass spectrometer (Waters, Milford, MA, USA).
  • Preparative HPLC was performed using a Waters prep-LC system consisting of a 2998 PDA detector and a 2545Q gradient module equipped with a YMC-Pack ODS-A semipreparative column (21.2 ⁇ 250 mm, 5 ⁇ m, 120 ⁇ , YMC, Kyoto, Japan).
  • Column chromatography was performed using Selekt flash chromatography with Biotage SNAP Ultra 100 g and SNAP Ultra C18 120 g pre-packed cartridges and 10 g Biotage SNAP dry rod cartridges manually packed with Sephadex LH-20 gel (MilliporeSigma, St. Louis, MO, USA). system (Biotage, Uppsala, Sweden).
  • the dried seshin root was obtained from KMD Medicinal Herbs Co. in 2019. (Ulsan, South Korea) and deposited at the Herbal Medicine Resources Research Center of the Korea Institute of Oriental Medicine (voucher number 2-19-0399).
  • Asaroidoxazine B (Compound 1): yellowish crystals; ECD ( c 0.5 mM, CH 3 CN) ⁇ +3.97 (289), -5.44 (223); 1 H (DMSO- d 6 , 600 MHz) and 13 C (DMSO- d 6 , 150 MHz) NMR data are shown in Table 1.
  • Compound 1 was obtained as yellow crystals, and a molecular ion was observed at 451.0573 [M + Na] + in HRESIMS.
  • the 1D NMR spectrum of compound 1 was similar to that of asaroidoxazine B.
  • Asaroidoxazine B is an aristolochic acid derivative containing an oxazine ring and has recently been reported to be isolated from the genus Asarum .
  • the presence of oxazine rings is difficult to confirm from NMR data. Therefore, in order to clarify the structure of Compound 1, the structure of Compound 1 was further confirmed using X-ray crystallography.
  • the X-ray structure of Fig. 2 clearly showed the presence of oxazine rings.
  • SKMEL5 melanoma
  • A431 non-melanoma skin
  • PC3 prostate
  • A549 lung
  • HCT116 colon
  • AGS gastric
  • MCF7 breast cancer
  • K562 leukemia
  • U-87 glioma
  • ACHN renal adenocarcinoma
  • Caki clear cell renal cancer
  • MTT ((4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide reagent (Sigma-Aldrich, Inc., St. Louis, MO, USA) was added to each well.
  • the extract in Table 2 is a 70% ethanol sessile root extract obtained in the process of preparing the sesshen root fraction of Example 1-2.
  • asaroidoxazine B exhibited strong inhibitory activity with a much stronger nanomolar IC 50 value than 5-FU in all cancer cell lines.
  • asaroidoxazine B exhibited potent cytotoxic activity with IC 50 values ranging from 129.5 to 755.2 nM against all cells tested.
  • an agar mix (0.5%) containing BME, FBS, glutamine, gentamycin, PBS and 1.25% agar was mixed with EGF (10 ng/mL) and asaroidoxazine B at each concentration, 3ml per well in a 6-well plate. It was hardened by dividing it into portions (bottom agar).
  • Mouse skin epithelial cells JB6 (ATCC, USA) were detached by treatment with trypsin, washed with 1X PBS, resuspended in 10% BME, and the number of cells was confirmed.
  • the test group treated with asaroidoxazine B exhibited an inhibition rate of 31.1 to 73.4% compared to the EGF-treated group in the EGF-induced carcinogenesis model, and asaroidoxazine B concentration-dependently. It was confirmed that the inhibition rate increased. Through this, it was confirmed that asaroidoxazine B has an effect of inhibiting or delaying tumor development.

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Abstract

The present invention relates to a use of Asaroidoxazine B having anticancer activity and, more specifically, to: a pharmaceutical composition and a health functional food composition, each utilizing Asaroidoxazine B represented by chemical formula 1, an isomer thereof, or a pharmaceutically acceptable salt thereof; a method for preventing, alleviating, or treating cancer, using same; and a method for isolation of the Asaroidoxazine B.

Description

항암 활성을 갖는 아사로이독사진 B의 용도 Use of asaroidoxazine B with anticancer activity
본 발명은 항암 활성을 갖는 아사로이독사진 B (Asaroidoxazine B)의 용도에 관한 것으로, 보다 상세하게는 하기 화학식 1로 표시되는 아사로이독사진 B, 이의 이성질체 또는 약학적으로 허용 가능한 염을 이용한 약학 조성물과 건강기능식품 조성물; 이를 이용한 암의 예방, 개선 또는 치료 방법; 및 상기 아사로이독사진 B의 분리방법을 제공한다.The present invention relates to the use of asaroidoxazine B having anticancer activity, and more particularly, to a pharmaceutical composition using asaroidoxazine B represented by the following formula (1), an isomer or a pharmaceutically acceptable salt thereof, and health functional food composition; a method for preventing, improving or treating cancer using the same; and a method for separating the asaroidoxazine B.
세신 (Asarum heterotropoides Fr.Schmidt var. mandshuricum (Maxim.) Kitag.)은 쥐방울덩굴 (Aristolochiaceae)과에 속하며 한국, 중국, 일본 등 아시아에 주로 분포한다. 세신 (A. heterotropoides)의 뿌리와 근경은 일부 아시아 국가에서 기침, 두통, 치통, 아프타성 구내염, 염증, 국소 마취 및 류머티즘을 치료하기 위해 전통 의학에서 사용되어 왔다[R. Zhou, "Resource science of Chinese medicinal materials", China Medical and Pharmaceutical Sciences Press, Beijing (1993); J. Lee et al., "Bioactive compounds from the roots of Asiasarum heterotropoides", Molecules 19 (1) (2014) 122-138]. 이전의 약리학적 연구에서는 세신 추출물이 항통각, 항염증, 살비 (acaricidal) 및 살충 (larvicidal) 활성이 있는 것으로 보고되었다 [Y. Xu et al., "Assessment on anti-nociception and anti-inflammation pharmacodynamics of Asarum heterotropoides var. mandshuricum and Asarum sieboldii", China J. Chin. Mat. Med. 37 (5) (2012) 625-631; J.-R. Kim et al., "Acaricidal activity of Asarum heterotropoides root-derived compounds and hydrodistillate constitutes toward Dermanyssus gallinae (Mesostigmata: Dermanyssidae)", Exp. Appl. Acarol. 68 (4) (2016) 485-495; H. Perumalsamy et al., "Larvicidal activity of Asarum heterotropoides root constituents against insecticide-susceptible and-resistant Culex pipiens pallens and Aedes aegypti and Ochlerotatus togoi", J. Agric. Food Chem. 58 (18) (2010) 10001-10006.] 또한, 이 식물체의 에센셜 오일은 항균 및 탈취 활성을 나타내는 것으로 보고되었다 [J. Moon, et al., "Physicochemical characterization and deodorant activity of essential oil recovered from Asiasarum heterotropoides using supercritical carbon dioxide and organic solvents", J. Ind. Eng. Chem. 69 (2019) 217-224; Y. Dan et al., "Activities of essential oils from Asarum heterotropoides var. mandshuricum against five phytopathogens", Crop Prot. 29 (3) (2010) 295-299]. 세신에 대한 일부 식물화학적 조사는 항염증, 세포독성 및 항암과 같은 유용한 생물학적 효과를 갖는 다양한 유형의 리그난, 모노테르펜, 아미드, 플라바논, 페난트렌, 벤젠 유도체 및 휘발성 페닐프로펜의 존재를 밝혀냈다[J. Lee et al., Bioactive compounds from the roots of Asiasarum heterotropoides, Molecules 19 (1) (2014) 122-138; Y. Jing et al., Chemical constituents from the roots and rhizomes of Asarum heterotropoides var. mandshuricum and the in vitro anti-inflammatory activity, Molecules 22 (1) (2017) 125; Y. Jing et al., Phenanthrene derivatives from roots and rhizomes of Asarum heterotropoides var. mandshuricum, Fitoterapia 117 (2017) 101-108; Y. Wang et al., Toxic and Repellent Effects of Volatile Phenylpropenes from Asarum heterotropoides on Lasioderma serricorne and Liposcelis bostrychophila, Molecules 23 (9) (2018) 2131]. 그러나 전통적인 의학적 중요성과 생물학적 효과에도 불구하고 세신 및 관련 대사 산물의 항암 활성에 대한 연구는 여전히 부족한 상태이다.Sesin ( Asarum heterotropoides Fr.Schmidt var. mandshuricum (Maxim.) Kitag.) belongs to the Aristolochiaceae family and is mainly distributed in Asia, including Korea, China, and Japan. The roots and rhizomes of A. heterotropoides have been used in traditional medicine in some Asian countries to treat cough, headache, toothache, aphthous stomatitis, inflammation, local anesthesia and rheumatism [R. Zhou, "Resource science of Chinese medicinal materials", China Medical and Pharmaceutical Sciences Press, Beijing (1993); J. Lee et al., "Bioactive compounds from the roots of Asiasarum heterotropoides", Molecules 19 (1) (2014) 122-138]. In previous pharmacological studies, it was reported that secin extract has anti-nociceptive, anti-inflammatory, acaricidal and larvicidal activities [Y. Xu et al., "Assessment on anti-nociception and anti-inflammation pharmacodynamics of Asarum heterotropoides var. mandshuricum and Asarum sieboldii", China J. Chin. Mat. Med. 37 (5) (2012) 625-631; J. -R. Kim et al., "Acaricidal activity of Asarum heterotropoides root-derived compounds and hydrodistillate constitutes toward Dermanyssus gallinae (Mesostigmata: Dermanyssidae)", Exp. Appl. Acarol. 68 (4) (2016) 485-495; H. Perumalsamy et al., "Larvicidal activity of Asarum heterotropoides root constituents against insecticide-susceptible and-resistant Culex pipiens pallens and Aedes aegypti and Ochlerotatus togoi", J. Agric. Food Chem. 58 (18) (2010) 10001-10006.] In addition, the essential oil of this plant has been reported to exhibit antimicrobial and deodorizing activities [J. Moon, et al., "Physicochemical characterization and deodorant activity of essential oil recovered from Asiasarum heterotropoides using supercritical carbon dioxide and organic solvents", J. Ind. Eng. Chem. 69 (2019) 217-224; Y. Dan et al., "Activities of essential oils from Asarum heterotropoides var. mandshuricum against five phytopathogens", Crop Prot. 29 (3) (2010) 295-299]. Some phytochemical investigations of secin revealed the existence of various types of lignans, monoterpenes, amides, flavanones, phenanthrenes, benzene derivatives and volatile phenylpropenes with useful biological effects such as anti-inflammatory, cytotoxic and anticancer [ J. Lee et al., Bioactive compounds from the roots of Asiasarum heterotropoides, Molecules 19 (1) (2014) 122-138; Y. Jing et al., Chemical constituents from the roots and rhizomes of Asarum heterotropoides var. mandshuricum and the in vitro anti-inflammatory activity, Molecules 22 (1) (2017) 125; Y. Jing et al., Phenanthrene derivatives from roots and rhizomes of Asarum heterotropoides var. mandshuricum, Fitoterapia 117 (2017) 101-108; Y. Wang et al., Toxic and Repellent Effects of Volatile Phenylpropenes from Asarum heterotropoides on Lasioderma sericorne and Liposcelis bostrychophila, Molecules 23 (9) (2018) 2131]. However, despite their traditional medical importance and biological effects, studies on the anticancer activity of secin and related metabolites are still lacking.
이러한 배경 하에, 본 발명자들은 세신의 뿌리에서 아사로이독사진 B (Asaroidoxazine B) 화합물을 분리하고, 이의 인간 암 세포주에 대한 억제 효과를 확인함으로써, 본 발명을 완성하였다.Under this background, the inventors of the present invention completed the present invention by isolating an asaroidoxazine B compound from the roots of sesame seeds and confirming its inhibitory effect on human cancer cell lines.
따라서, 본 발명은 세신 뿌리로부터 분리한 화합물을 이용한 항암용 조성물을 제공하는 것을 목적으로 한다.Accordingly, an object of the present invention is to provide an anti-cancer composition using a compound isolated from the secinum root.
상기 용도와 관련하여, 본 발명의 다른 목적은 세신 뿌리로부터 분리한 화합물을 이용한 암의 예방, 개선 또는 치료 방법을 제공하는 것이다.In connection with the above use, another object of the present invention is to provide a method for preventing, ameliorating or treating cancer using a compound isolated from the root of Sesin.
상기 용도와 관련하여, 본 발명의 또 다른 목적은 암의 예방, 개선 또는 치료를 위한 약제 또는 건강기능식품의 제조 시, 세신 뿌리로부터 분리한 화합물의 용도를 제공하는 것이다.In connection with the above use, another object of the present invention is to provide the use of a compound isolated from the root of Sesin in the preparation of a pharmaceutical or health functional food for the prevention, improvement or treatment of cancer.
본 발명의 또 다른 목적은 세신 뿌리로부터 항암 활성을 갖는 화합물을 분리하는 방법을 제공하는 것이다.Another object of the present invention is to provide a method for isolating a compound having anti-cancer activity from secin root.
상술한 과제를 해결하기 위해, 본 발명은 하기 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 약학적으로 허용 가능한 염을 유효성분으로 포함하는 암 예방 또는 치료용 약학 조성물을 제공한다:In order to solve the above problems, the present invention provides a pharmaceutical composition for preventing or treating cancer comprising Asaroidoxazine B represented by Formula 1 below, an isomer thereof or a pharmaceutically acceptable salt thereof as an active ingredient. do:
[화학식 1][Formula 1]
Figure PCTKR2022021008-appb-img-000001
.
Figure PCTKR2022021008-appb-img-000001
.
추가로, 본 발명은 전술한 화합물, 이의 이성질체 또는 식품학적으로 허용 가능한 염을 유효성분으로 포함하는 암 예방 또는 개선용 건강기능식품 조성물을 제공한다.In addition, the present invention provides a health functional food composition for preventing or improving cancer comprising the aforementioned compound, its isomer or food-acceptable salt as an active ingredient.
상기 용도와 관련하여, 본 발명은 추가로 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 약학적으로 허용 가능한 염을 이를 필요로 하는 개체에게 투여하는 것을 포함하는, 암의 예방, 개선 또는 치료 방법을 제공한다.In relation to the above use, the present invention further provides for the prevention of cancer, comprising administering Asaroidoxazine B represented by Formula 1, an isomer or a pharmaceutically acceptable salt thereof to a subject in need thereof. , improvement or treatment methods are provided.
상기 용도와 관련하여, 본 발명은 추가로 암의 예방, 개선 또는 치료를 위한 약제 또는 건강기능식품의 제조 시 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 이의 약학 또는 식품학적으로 허용 가능한 염의 용도를 제공한다.Regarding the above use, the present invention further relates to asaroidoxazine B represented by Formula 1, an isomer thereof, or a pharmaceutical or food product thereof when preparing a pharmaceutical or health functional food for the prevention, improvement or treatment of cancer. Provided are the uses of acceptable salts.
본 발명의 바람직한 일 실시예에 따르면, 상기 이성질체는 라세미체, 거울상 이성질체, 부분입체 이성질체, 거울상 이성질체의 혼합물 또는 부분입체 이성질체의 혼합물을 포함할 수 있다.According to a preferred embodiment of the present invention, the isomers may include racemates, enantiomers, diastereomers, mixtures of enantiomers or mixtures of diastereomers.
본 발명의 바람직한 다른 일 실시예에 따르면, 상기 아사로이독사진 B는 세신 (Asarum heterotropoides) 뿌리로부터 분리될 수 있다.According to another preferred embodiment of the present invention, the Asaroidoxazine B may be isolated from the roots of Asarum heterotropoides .
본 발명의 바람직한 또 다른 일 실시예에 따르면, 상기 아사로이독사진 B, 이의 이성질체 또는 이의 약학 또는 식품학적으로 허용 가능한 염은 암세포를 억제하거나, 종양 발생을 억제 또는 지연시키는 효과를 나타낼 수 있다.According to another preferred embodiment of the present invention, the asaroidoxazine B, an isomer thereof, or a pharmaceutically or food-acceptable salt thereof may exhibit an effect of inhibiting cancer cells or inhibiting or delaying tumor development.
본 발명은 또한, 다음의 단계를 포함하는, 세신 (Asarum heterotropoides) 뿌리로부터 상기 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B)를 분리하는 방법을 제공한다:The present invention also provides a method for isolating Asaroidoxazine B represented by Formula 1 from Asarum heterotropoides roots, comprising the following steps:
(a) 세신 뿌리를 에탄올 용매로 추출하여 세신 뿌리 에탄올 추출물을 수득하는 단계;(a) extracting the sesame root with an ethanol solvent to obtain an ethanol extract of the sesame root;
(b) 상기 세신 뿌리 에탄올 추출물을 헥산, 에틸아세테이트 및 물 순서로 계통 분획하여 에틸아세테이트 분획물을 수득하는 단계;(b) obtaining an ethyl acetate fraction by systematically fractionating the ethanol extract of the sesame root in the order of hexane, ethyl acetate and water;
(c) 상기 에틸아세테이트 분획물을 물-메탄올 혼합액을 유출 용매로 하여 플래쉬 크로마토그래피를 수행하여 분획물을 수득하는 단계; 및(c) obtaining fractions by performing flash chromatography on the ethyl acetate fraction using a water-methanol mixture as an effluent solvent; and
(d) 컬럼 크로마토그래피를 수행한 후 수득된 분획물을 물-아세토니트릴 혼합액을 유출 용매로 하여 반분취 크로마토그래피를 수행하여 상기 아사로이독사진 B를 정제하는 단계.(d) purifying the asaroidoxazine B by performing semipreparative chromatography on the fraction obtained after column chromatography using a water-acetonitrile mixture as an effluent solvent.
본 발명의 바람직한 일 실시예에 따르면, 상기 (a) 단계의 에탄올 용매는 50 내지 90% 에탄올 용매일 수 있다.According to a preferred embodiment of the present invention, the ethanol solvent in step (a) may be 50 to 90% ethanol solvent.
본 발명의 바람직한 다른 일 실시예에 따르면, 상기 (c) 단계의 물-메탄올 혼합액은 6:4에서 1:9의 농도 구배로 적용될 수 있다.According to another preferred embodiment of the present invention, the water-methanol mixture in step (c) may be applied with a concentration gradient of 6:4 to 1:9.
본 발명의 바람직한 또 다른 일 실시예에 따르면, 상기 (d) 단계의 물-아세토니트릴 혼합액은 6.5:3.5 내지 5.5:4.5의 농도 구배로 적용될 수 있다.According to another preferred embodiment of the present invention, the water-acetonitrile mixture of step (d) may be applied with a concentration gradient of 6.5:3.5 to 5.5:4.5.
본 발명에서 제공되는 아사로이독사진 B는 기존 항암제인 5-FU에 비해 현저히 낮은 농도에서도 우수한 항암 활성을 나타내는 바, 암의 예방, 개선 또는 치료에 효과적으로 사용될 수 있으며, 과량의 사용에 따른 부작용을 방지할 수 있다.Asaroidoxazine B provided in the present invention exhibits excellent anticancer activity even at a significantly lower concentration than 5-FU, an existing anticancer drug, and can be effectively used for preventing, improving or treating cancer, and preventing side effects due to excessive use can do.
도 1은 세신으로부터 분리된 화합물 1 (아사로이독사진 B)의 화학 구조를 나타낸 것이다.Figure 1 shows the chemical structure of compound 1 (asaroidoxazine B) isolated from secin.
도 2는 화합물 1 (아사로이독사진 B)의 X선 ORTEP 도면을 나타낸 것이다.Figure 2 shows an X-ray ORTEP diagram of compound 1 (asaroidoxazine B).
도 3은 화합물 1 (아사로이독사진 B)의 계산적 및 실험적 ECD 스펙트럼을 나타낸 것이다.Figure 3 shows the computational and experimental ECD spectra of compound 1 (asaroidoxazine B).
도 4a 내지 4f는 다양한 암세포주에 대한 화합물 1 (아사로이독사진 B)의 세포 증식 억제 효과를 MTT 어세이로 측정한 결과를 나타낸 것으로, 도 4a는 인간 흑색종 세포, 도 4b는 비흑색종 피부암 세포, 도 4c는 인간 전립선암 세포, 도 4d는 인간 폐암 세포, 도 4e는 인간 대장암 세포 및 도 4f는 인간 위암 세포, 도 4g는 인간 유방 선암종 세포, 도 4h는 인간 백혈병 세포, 도 4i는 인간 뇌 교모세포종 세포, 도 4j는 인간 신장샘암종 세포, 도 4k는 투명세포 신장암에 대한 세포 증식 억제 효과를 보여준다.Figures 4a to 4f show the results of measuring the cell proliferation inhibitory effect of compound 1 (asaroidoxazin B) on various cancer cell lines by MTT assay, Figure 4a is human melanoma cells, Figure 4b is non-melanoma skin cancer cells, FIG. 4C is human prostate cancer cells, FIG. 4D is human lung cancer cells, FIG. 4E is human colon cancer cells and FIG. 4F is human gastric cancer cells, FIG. 4G is human breast adenocarcinoma cells, FIG. 4H is human leukemia cells, FIG. 4I is Human brain glioblastoma cells, Fig. 4j shows the cytostatic effect on human renal adenocarcinoma cells, Fig. 4k shows clear cell renal carcinoma.
도 5는 화합물 1 (아사로이독사진 B)의 EGF-유도 세포 형질전환 콜로니 성장 저해 효과를 평가한 결과이다.5 shows the results of evaluating the effect of compound 1 (asaroidoxazin B) on inhibiting the growth of EGF-induced cell transformed colonies.
이하에서는, 본 발명을 더욱 상세히 설명한다.In the following, the present invention will be described in more detail.
한편, 본원에서 개시되는 각각의 설명 및 실시형태는 각각의 다른 설명 및 실시 형태에도 적용될 수 있다. 즉, 본원에서 개시된 다양한 요소들의 모든 조합이 본 발명의 범주에 속한다. 또한, 하기 기술되는 구체적인 서술에 의하여 본 발명의 범주가 제한된다고 할 수 없다.Meanwhile, each description and embodiment disclosed herein may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be said that the scope of the present invention is limited by the specific description described below.
또한, 당해 기술분야의 통상의 지식을 가진 자는 통상의 실험만을 사용하여 본 출원에 기재된 본 발명의 특정 양태에 대한 다수의 등가물을 인지하거나 확인할 수 있다. 또한, 이러한 등가물은 본 발명에 포함되는 것으로 의도된다. In addition, those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described in this application. Also, such equivalents are intended to be included in this invention.
상술한 바와 같이, 본 발명자들은 세신의 뿌리에서 아사로이독사진 B 화합물을 분리하고, 이의 인간 암 세포주에 대한 우수한 억제 효과를 확인하였다.As described above, the present inventors isolated the asaroidoxazine B compound from the roots of sesame seeds and confirmed its excellent inhibitory effect on human cancer cell lines.
따라서, 본 발명의 제1 측면은 하기 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 약학적으로 허용 가능한 염을 유효성분으로 포함하는 암 예방 또는 치료용 약학 조성물; 및 하기 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 식품학적으로 허용 가능한 염을 유효성분으로 포함하는 암 예방 또는 개선용 건강기능식품 조성물에 관한 것이다:Accordingly, a first aspect of the present invention is a pharmaceutical composition for preventing or treating cancer comprising asaroidoxazine B represented by Formula 1 below, an isomer thereof or a pharmaceutically acceptable salt thereof as an active ingredient; And asaroidoxazine B represented by the following formula (1), an isomer thereof, or a food-acceptable salt thereof as an active ingredient. It relates to a health functional food composition for preventing or improving cancer:
[화학식 1][Formula 1]
Figure PCTKR2022021008-appb-img-000002
.
Figure PCTKR2022021008-appb-img-000002
.
본 발명에서 정의되는 상기 세신은 족두리풀 (Asarum heterotropoides Fr. mandshuricum), 민족두리풀 (Asarum sieboldi Miq), 또는 섬족두리풀 (Asarum maculatum)일 수 있고, 바람직하게는 족두리풀 (Asarum heterotropoides Fr. mandshuricum)일 수 있다.The body as defined in the present invention may be Asarum heterotropoides Fr. mandshuricum, ethnic sieboldi Miq, or Asarum maculatum, preferably Asarum heterotropoides Fr. mandshuricum. .
본 발명의 일 실시예에서, 상기 이성질체는 라세미체, 거울상 이성질체, 부분입체 이성질체, 거울상 이성질체의 혼합물 또는 부분입체 이성질체의 혼합물을 포함할 수 있으나, 이로 한정되지 않는다.In one embodiment of the present invention, the isomer may include, but is not limited to, a racemate, an enantiomer, a diastereomer, a mixture of enantiomers, or a mixture of diastereomers.
본 발명의 다른 일 실시예에서, 상기 아사로이독사진 B는 세신 (Asarum heterotropoides)으로부터 분리될 수 있으며, 바람직하게는 뿌리로부터 분리될 수 있다. 상기 세신은 상업적으로 판매되는 것을 구입하거나, 자연에서 채취 또는 재배된 것을 사용할 수 있다.In another embodiment of the present invention, the asaroidoxazine B may be isolated from Asarum heterotropoides , preferably from roots. The body wash can be purchased commercially, or used collected or cultivated in nature.
상기 암은, 예를 들어, 흑색종, 비흑색종 피부암 (예를 들어, 편평상피세포암), 유방암, 두경부암, 갑상선암, 섬유육종, 연부조직육종, 골육종, 고환암, 전립선암, 난소암, 방광암, 피부암, 뇌암, 신경교종, 혈관육종, 비만세포종, 백혈병, 림프종, 간암, 폐암, 췌장암, 위암, 신장암, 대장암, 조혈 종양, 신경 모세포종, 표피암종 또는 이의 전이암일 수 있으나, 이로 제한되지 않는다.Such cancers include, for example, melanoma, non-melanoma skin cancer (eg, squamous cell carcinoma), breast cancer, head and neck cancer, thyroid cancer, fibrosarcoma, soft tissue sarcoma, osteosarcoma, testicular cancer, prostate cancer, ovarian cancer, Bladder cancer, skin cancer, brain cancer, glioma, hemangiosarcoma, mastocytoma, leukemia, lymphoma, liver cancer, lung cancer, pancreatic cancer, stomach cancer, kidney cancer, colorectal cancer, hematopoietic tumor, neuroblastoma, epidermal carcinoma, or metastasis thereof, but is limited thereto. It doesn't work.
본 발명의 구체적인 일 실시예에서는, 세신 뿌리로부터 아사로이독사진 B를 분리하여 이의 항암 활성을 확인하였다. 그 결과, 표 2, 도 4a 내지 도 4k에서 확인되는 바와 같이 아사로이독사진 B는 흑색종, 비흑색종 피부암, 전립선암, 폐암, 대장암, 위암, 유방암, 백혈병, 신경교종 및 신장암에 대해 증식 억제 활성을 나타냈다. 특히, 나노몰 (nM) 수준에서도 세포 증식 억제 효과를 나타내어 마이크로몰 (μM) 수준에서 효과를 나타내는 양성 대조군 5-FU에 비해 현저히 우수한 항암 활성을 가지는 것으로 확인되었다.In a specific embodiment of the present invention, asaroidoxazine B was isolated from the roots of sesame seeds and its anticancer activity was confirmed. As a result, as confirmed in Table 2 and FIGS. 4a to 4k, asaroidoxazine B is effective against melanoma, non-melanoma skin cancer, prostate cancer, lung cancer, colon cancer, gastric cancer, breast cancer, leukemia, glioma and renal cancer. showed antiproliferative activity. In particular, it was found to have significantly superior anticancer activity compared to the positive control 5-FU, which exhibits a cell proliferation inhibitory effect even at the nanomolar (nM) level and exhibits an effect at the micromolar (μM) level.
본 발명의 다른 구체적인 일 실시예에서는, 상피세포 성장인자(epidermal growth factor, EGF)-유도 발암 모델에서 세신 뿌리로부터 분리된 아사로이독사진 B의 항암 활성을 평가하였다. 그 결과, 도 5에서 확인되는 바와 같이 아사로이독사진 B는 EGF-유도 발암 모델에서 EGF 처리군에 비해 31.1 내지 73.4%의 저해율을 나타내었고, 아사로이독사진 B의 농도 의존적으로 저해율이 증가되었다. 이를 통해, 아사로이독사진 B가 EGF에 의한 세포 형질전환을 억제하여 종양 발생을 억제 또는 지연시킴으로써 암 예방 효과를 나타냄을 알 수 있다.In another specific embodiment of the present invention, the anticancer activity of asaroidoxazine B isolated from sesame root was evaluated in an epidermal growth factor (EGF)-induced carcinogenesis model. As a result, as confirmed in FIG. 5, asaroidoxazine B showed an inhibition rate of 31.1 to 73.4% compared to the EGF-treated group in the EGF-induced carcinogenesis model, and the inhibition rate was increased in a concentration-dependent manner of asaroidoxazine B. Through this, it can be seen that asaroidoxazine B exhibits cancer-preventive effects by suppressing or delaying tumor development by inhibiting EGF-induced cell transformation.
전술한 아사로이독사진 B는 세신 뿌리로부터 분리되므로, 이를 포함하는 세신 뿌리 추출물 또는 분획물 또한 암 예방, 개선 또는 치료에 활용될 수 있다.Since the above-described asaroidoxazine B is isolated from the sessile root, the sessile root extract or fraction containing it can also be used for preventing, improving or treating cancer.
본 발명에서 사용되는 용어, "추출물"은 상기 세신 뿌리의 추출처리에 의하여 얻어지는 추출액, 상기 추출액의 희석액이나 농축액, 상기 추출액을 건조하여 얻어지는 건조물, 상기 추출액의 조정제물이나 정제물, 또는 이들의 혼합물 등, 추출액 자체 및 추출액을 이용하여 형성 가능한 모든 제형의 추출물을 포함한다. 상기 추출물은 세신의 천연, 잡종 또는 변종 식물로부터 추출될 수 있고, 식물 조직 배양물로부터도 추출이 가능하다.As used in the present invention, the term "extract" refers to an extract obtained by the extraction treatment of the root of the shoot, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a purified product or a purified product of the extract, or a mixture thereof. Etc., the extract itself and extracts of all formulations that can be formed using the extract. The extract may be extracted from a natural, hybrid or mutant plant of seshin, and may also be extracted from plant tissue culture.
상기 추출물은 헥산, 클로로포름, 메틸렌클로리드, 에틸아세테이트, 아세톤, C1 내지 C4의 저급 알코올, 물 또는 이들의 혼합 용매를 추출 용매로 하여 제조될 수 있고, 바람직하게는 C1 내지 C4의 저급 알코올, 물 또는 이들의 혼합 용매를 추출 용매로 하여 제조될 수 있으나, 이로 한정되지 않는다.The extract may be prepared by using hexane, chloroform, methylene chloride, ethyl acetate, acetone, C 1 to C 4 lower alcohol, water or a mixed solvent thereof as an extraction solvent, preferably C 1 to C 4 It may be prepared by using a lower alcohol, water or a mixed solvent thereof as an extraction solvent, but is not limited thereto.
본 발명의 조성물에 있어서, 세신 추출물은 상기 추출 용매를 사용하여 건조된 세신 뿌리를 1회 이상 추출하여 제조될 수 있으며, 상기 용매 추출물을 감압농축한 후 동결 건조 또는 분무 건조하여 얻은 건조 추출물로 제조할 수 있다.In the composition of the present invention, the extract of the extract may be prepared by extracting the dried roots of the shoot at least once using the extraction solvent, and the solvent extract is concentrated under reduced pressure and then prepared as a dry extract obtained by freeze-drying or spray drying. can do.
본 발명에서 사용되는 용어, "분획물"은 여러 다양한 구성 성분들을 포함하는 혼합물로부터 특정 성분 또는 특정 성분 그룹을 분리하기 위하여 분획을 수행하여 얻어진 결과물을 의미한다.As used herein, the term "fraction" refers to a result obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.
예를 들어, 아사로이독사진 B를 포함하는 세신 뿌리 분획물은 (a) 세신 뿌리를 에탄올 용매로 추출하여 세신 뿌리 에탄올 추출물을 수득하는 단계; 및 (b) 상기 세신 뿌리 에탄올 추출물을 헥산, 에틸아세테이트 및 물 순서로 계통 분획하여 에틸아세테이트 분획물을 수득하는 단계를 수행하여 수득될 수 있으나, 이로 한정되지 않는다. 상기 (a) 단계에서, 에탄올 용매는 50 내지 90% 에탄올, 바람직하게는 60 내지 80% 에탄올, 가장 바람직하게는 65 내지 75% 에탄올 용매일 수 있다. 에탄올 이외의 다른 용매를 사용하는 경우, 상기 아사로이독사진 B 함량이 급격하게 감소하여, 의도한 항암 활성이 나타나지 않을 수 있다.For example, the sessile root fraction containing asaroidoxazine B may be obtained by (a) extracting the sessile root with an ethanol solvent to obtain an ethanol extract of the sessile root; And (b) it can be obtained by carrying out the step of obtaining an ethyl acetate fraction by systematically fractionating the secinum root ethanol extract in the order of hexane, ethyl acetate and water, but is not limited thereto. In step (a), the ethanol solvent may be 50 to 90% ethanol, preferably 60 to 80% ethanol, and most preferably 65 to 75% ethanol. In the case of using a solvent other than ethanol, the content of the asaroidoxazine B is rapidly reduced, and the intended anticancer activity may not be exhibited.
본 발명의 조성물에 있어서, 용어 "예방"은 질병 또는 병증의 발병을 억제하거나 지연시키는 모든 행위를 의미한다. 본 발명에 있어서는 암의 발병 시기를 지연시키거나, 발병을 억제하는 것을 의미한다.In the composition of the present invention, the term "prevention" means any action to suppress or delay the onset of a disease or condition. In the present invention, it means delaying the onset of cancer or suppressing the onset of cancer.
본 발명의 조성물에 있어서, 용어 "개선"은 질병 또는 병증 상태를 호전 또는 이롭게 변경하는 모든 행위를 의미하는 것으로, 본 발명에 있어서는 암의 증상을 호전시키는 것을 의미한다.In the composition of the present invention, the term "improvement" means any action that improves or beneficially changes a disease or condition, and in the present invention, it means improving the symptoms of cancer.
본 발명의 조성물에 있어서, 용어 "치료"는 질병 또는 병증의 진행을 지연, 중단 또는 역전시키는 모든 행위를 의미하는 것으로, 본 발명에 있어서는 암의 증상을 경감, 완화 또는 없애거나, 역전시키는 것을 의미한다.In the composition of the present invention, the term "treatment" means any action that delays, stops, or reverses the progression of a disease or condition, and in the present invention, it means reducing, alleviating, eliminating, or reversing the symptoms of cancer. do.
상기 용도와 관련하여, 본 발명은 추가로 상기 화학식 1로 표시되는 아사로이독사진 B, 이의 이성질체 또는 약학적으로 허용 가능한 염을 이를 필요로 하는 개체에게 투여하는 것을 포함하는, 암의 예방, 개선 또는 치료 방법에 관한 것이다.Regarding the above use, the present invention further relates to the prevention, improvement or prevention of cancer, comprising administering asaroidoxazine B, an isomer or a pharmaceutically acceptable salt thereof represented by Formula 1 above to a subject in need thereof. It is about treatment methods.
상기 용도와 관련하여, 본 발명은 추가로 암의 예방, 개선 또는 치료를 위한 약제 또는 건강기능식품의 제조 시 화학식 1로 표시되는 아사로이독사진 B, 이의 이성질체 또는 이의 약학 또는 식품학적으로 허용 가능한 염의 용도에 관한 것이다.Regarding the above use, the present invention further relates to the use of asaroidoxazine B represented by Formula 1, an isomer thereof, or a pharmaceutically or foodtically acceptable salt thereof in the preparation of a pharmaceutical or health functional food for the prevention, improvement or treatment of cancer. It's about use.
본 발명의 방법 및 용도에 있어서, 상기 아사로이독사진 B, 이의 이성질체 또는 약학 또는 식품학적으로 허용 가능한 염 및 이의 효과에 대한 설명은 전술한 바와 동일하므로, 그 기재를 생략한다.In the methods and uses of the present invention, descriptions of asaroidoxazine B, its isomers or pharmaceutically or food-acceptable salts, and their effects are the same as those described above, so description thereof will be omitted.
본 발명에서 사용되는 용어 "약학적으로 허용 가능한 염"이란, 환자에게 비교적 비독성이고 무해한 유효작용을 갖는 농도로서 이 염에 기인한 부작용이 화학식 1의 화합물의 이로운 효능을 떨어뜨리지 않는 화학식 1의 화합물의 어떠한 유기 또는 무기 부가염을 의미한다. 이들 염은 유리산으로는 무기산과 유기산을 사용할 수 있으며, 무기산으로는 염산, 브롬산, 질산, 황산, 과염소산, 인산 등을 사용할 수 있고, 유기산으로는 구연산, 초산, 젖산, 말레산, 푸마린산, 글루콘산, 메탄설폰산, 글리콘산, 숙신산, 타타르산, 갈룩투론산, 엠본산, 글루탐산, 아스파르트산, 옥살산, (D) 또는 (L) 말산, 말레산, 메테인설폰산, 에테인설폰산, 4-톨루엔술폰산, 살리실산, 시트르산, 벤조산 또는 말론산 등을 사용할 수 있다. 또한, 이들 염은 알칼리 금속염(나트륨염, 칼륨염 등) 및 알칼리 토금속염(칼슘염, 마그네슘염 등) 등을 포함한다. 예를 들면, 산부가염으로는 아세테이트, 아스파테이트, 벤즈에이트, 베실레이트, 바이카보네이트/카보네이트, 바이설페이트/설페이트, 보레이트, 캄실레이트, 시트레이트, 에디실레이트, 에실레이트, 포메이트, 퓨마레이트, 글루셉테이트, 글루코네이트, 글루큐로네이트, 헥사플루오로포스페이트, 하이벤제이트, 하이드로클로라이드/클로라이드, 하이드로브로마이드/브로마이드, 하이드로요오디드/요오디드, 이세티오네이트, 락테이트, 말레이트, 말리에이트, 말로네이트, 메실레이트, 메틸설페이트, 나프틸레이트, 2-나프실레이트, 니코티네이트, 나이트레이트, 오로테이트, 옥살레이트, 팔미테이트, 파모에이트, 포스페이트/수소 포스페이트/이수소 포스페이트, 사카레이트, 스테아레이트, 석시네이트, 타르트레이트, 토실레이트, 트리플루오로아세테이트, 알루미늄, 알기닌, 벤자틴, 칼슘, 콜린, 디에틸아민, 디올아민, 글라이신, 라이신, 마그네슘, 메글루민, 올아민, 칼륨, 나트륨, 트로메타민, 아연염 등이 포함될 수 있으며, 이들 중 하이드로클로라이드 또는 트리플루오로아세테이트일 수 있다.As used herein, the term "pharmaceutically acceptable salt" refers to a concentration that has an effective effect that is relatively non-toxic and harmless to patients, and the side effects caused by the salt do not reduce the beneficial efficacy of the compound of Formula 1. means any organic or inorganic addition salt of a compound. For these salts, inorganic and organic acids can be used as free acids, hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, perchloric acid, and phosphoric acid can be used as inorganic acids, and citric acid, acetic acid, lactic acid, maleic acid, and fumarin as organic acids. Acids, gluconic acid, methanesulfonic acid, glycolic acid, succinic acid, tartaric acid, galacturonic acid, embonic acid, glutamic acid, aspartic acid, oxalic acid, (D) or (L) malic acid, maleic acid, methanesulfonic acid, ethanesul For example, phonic acid, 4-toluenesulfonic acid, salicylic acid, citric acid, benzoic acid or malonic acid may be used. In addition, these salts include alkali metal salts (sodium salt, potassium salt, etc.) and alkaline earth metal salts (calcium salt, magnesium salt, etc.) and the like. For example, acid addition salts include acetate, aspartate, benzate, besylate, bicarbonate/carbonate, bisulfate/sulfate, borate, camsylate, citrate, edisylate, esylate, formate, fumarate, Gluceptate, gluconate, glucuronate, hexafluorophosphate, hybenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, malic Eight, malonate, mesylate, methyl sulfate, naphthylate, 2-naphsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, saccharide Lates, stearates, succinates, tartrates, tosylates, trifluoroacetates, aluminum, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, Potassium, sodium, tromethamine, zinc salts and the like may be included, among which hydrochloride or trifluoroacetate may be used.
본 발명의 약학 조성물은 약학적으로 허용 가능한 담체를 추가적으로 포함할 수 있다.The pharmaceutical composition of the present invention may additionally include a pharmaceutically acceptable carrier.
본 발명의 약학 조성물은, 포유동물에 투여된 후 활성 성분의 신속, 지속 또는 지연된 방출을 제공할 수 있도록 당업계에 잘 알려진 방법을 사용하여 약학적 제형으로 제조될 수 있다. 제형의 제조에 있어서, 활성 성분을 담체와 함께 혼합 또는 희석하거나, 용기 형태의 담체 내에 봉입시키는 것이 바람직하다.The pharmaceutical composition of the present invention can be prepared into a pharmaceutical formulation using methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. In preparation of a dosage form, it is preferable to mix or dilute the active ingredient with a carrier or to encapsulate it in a carrier in the form of a container.
따라서, 본 발명의 약학 조성물은, 통상의 방법에 따라 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구형 제형, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있고, 조성물의 제조에 통상적으로 사용하는 적절한 담체, 부형제 및 희석제를 더 포함할 수 있다.Therefore, the pharmaceutical composition of the present invention is formulated according to conventional methods into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and sterile injection solutions. It may further include appropriate carriers, excipients and diluents commonly used in the preparation of the composition.
예를 들어, 본 발명의 약학 조성물에 포함될 수 있는 담체는 락토즈, 덱스트로즈, 수크로스, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유 등을 포함하나, 이에 제한되는 것은 아니다. 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제된다.For example, carriers that may be included in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants.
경구 투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 상기 화합물에 적어도 하나 이상의 부형제 예를 들어, 전분, 칼슘카보네이트(calcium carbonate), 수크로스(sucrose) 또는 락토오스 (lactose), 젤라틴 등을 섞어 조제된다. 또한 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 사용된다.Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one excipient, for example, starch, calcium carbonate, sucrose, etc. ) or by mixing lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used.
경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되고, 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들어 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다.Liquid preparations for oral use include suspensions, solutions for oral use, emulsions, syrups, etc., and various excipients such as wetting agents, sweeteners, aromatics, preservatives, etc. may be included in addition to water and liquid paraffin, which are commonly used simple diluents. there is.
비경구 투여를 위한 제제에는 멸균된 수용액, 비수성 용제, 현탁제, 유제, 동결건조 제제, 좌제가 포함된다. 비수성 용제, 현탁제로는 프로필렌글리콜 (propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로제라틴 등이 사용될 수 있다.Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogeratin and the like may be used.
본 발명에서 사용된 용어, "투여"는 어떠한 적절한 방법으로 환자에게 본 발명의 약학 조성물을 도입하는 것을 의미한다.As used herein, the term "administration" means introducing the pharmaceutical composition of the present invention to a patient by any suitable method.
본 발명에 따른 약학 조성물의 투여 방식은 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용하는 방식에 따를 수 있다. 상기 투여 방식은 목적 조직에 도달할 수 있는 한 제한되지 않으나, 복강 내 투여, 정맥 내 투여, 근육 내 투여, 피하 투여, 피내 투여, 경구 투여, 국소 투여, 비 내 투여될 수 있다. 본 발명에 따른 약학 조성물은 목적하는 투여 방식에 따라 다양한 제형으로 제작될 수 있다.The administration method of the pharmaceutical composition according to the present invention is not particularly limited, and may follow a method commonly used in the art. The administration method is not limited as long as it can reach the target tissue, but may be intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, or intranasal administration. The pharmaceutical composition according to the present invention may be prepared in various formulations depending on the desired administration method.
본 발명의 약학 조성물은 약학적으로 유효한 양으로 투여할 수 있다.The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount.
상기 "약학적으로 유효한 양"은 의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분 한 양을 의미하며, 유효 용량 수준은 개체 종류 및 중증도, 연령, 성별, 감염된 바이러스 종류, 약물의 활성, 약물에 대한 민감도, 투여 시간, 투여 경로 및 배출 비율, 치료 기간, 동시 사용되는 약물을 포함한 요소 및 기타 의학 분야에 잘 알려진 요소에 따라 결정될 수 있다.The "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level is subject type and severity, age, sex, infected virus type, drug activity, drug sensitivity, time of administration, route of administration and excretion rate, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field.
본 발명에 따른 약학 조성물의 통상적인 1일 투여량은 당업자가 적절하게 선택할 수 있으며, 1회 또는 수 회로 나누어 투여될 수 있다.A typical daily dose of the pharmaceutical composition according to the present invention can be appropriately selected by those skilled in the art, and can be administered once or divided into several times.
본 발명의 조성물을 매일 투여 또는 간헐적으로 투여해도 좋고, 1일당 투여 횟수는 1회 또는 2~3회로 나누어 투여하는 것이 가능하다. 또한, 본 발명의 조성물은 암의 예방 또는 치료를 위하여 단독으로, 또는 다른 약물 치료와 병용하여 사용할 수 있다. 상기 요소를 모두 고려하여 부작용 없이 최소한의 양으로 최대 효과를 얻을 수 있는 양을 투여하는 것이 중요하며, 당업자에 의해 용이하게 결정될 수 있다.The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into 2 to 3 times. In addition, the composition of the present invention can be used alone or in combination with other drug treatments for the prevention or treatment of cancer. It is important to administer the amount that can obtain the maximum effect with the minimum amount without side effects in consideration of all the above factors, and can be easily determined by those skilled in the art.
본 발명에서 사용되는 용어, "개체"란, 암이 발병되었거나 발병할 가능성이 있는 인간을 포함한 모든 동물을 의미한다. 상기 동물은 인간뿐만 아니라 이와 유사한 증상의 치료를 필요로 하는 소, 말, 양, 돼지, 염소, 낙타, 영양, 개, 고양이 등의 포유동물일 수 있으나, 이에 제한되지는 않는다.As used herein, the term "subject" refers to all animals, including humans, who have or are likely to develop cancer. The animal may be not only humans but also mammals such as cattle, horses, sheep, pigs, goats, camels, antelopes, dogs, and cats that require treatment for similar symptoms, but are not limited thereto.
본 발명에서 사용되는 용어 "식품"은 육류, 소시지, 빵, 초콜릿, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올음료, 비타민 복합제, 건강 기능 식품 및 건강 식품 등이 있으며, 통상적인 의미에서의 식품을 모두 포함한다.As used herein, the term "food" refers to meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcohol Beverages, vitamin complexes, health functional foods and health foods, etc., include all foods in a conventional sense.
본 발명의 건강기능식품 조성물은 환제, 분말, 과립, 침제, 정제, 캡슐 또는 액제 등의 형태를 포함하며, 본 발명의 조성물을 첨가할 수 있는 식품으로는, 예를 들어, 각종 식품류, 예를 들어, 음료, 껌, 차, 비타민 복합제, 건강보조 식품류 등이 있다.The health functional food composition of the present invention includes forms such as pills, powders, granules, precipitates, tablets, capsules or liquids, and as foods to which the composition of the present invention can be added, for example, various foods, such as For example, there are beverages, chewing gum, tea, vitamin complexes, and health supplements.
본 발명의 건강기능식품 조성물에 포함될 수 있는 필수 성분으로 아사로이독사진 B 및/또는 이를 포함하는 세신 뿌리 분획물을 함유하는 것 외에는 다른 성분에는 특별히 제한이 없으며, 통상의 식품과 같이 여러 가지 생약추출물, 식품 보조 첨가제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다.There are no particular restrictions on other ingredients except for containing asaroidoxazine B and/or a sesame root fraction containing the same as essential ingredients that may be included in the health functional food composition of the present invention, and various herbal extracts as in conventional foods, Food additives or natural carbohydrates may be included as additional ingredients.
또한, 상기 식품 보조 첨가제는 당업계에 통상적인 식품 보조 첨가제, 예를 들어 향미제, 풍미제, 착색제, 충진제, 안정화제 등을 포함한다.In addition, the food auxiliary additives include food auxiliary additives common in the art, such as flavoring agents, flavoring agents, coloring agents, fillers, stabilizers, and the like.
상기 천연 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스 등; 및 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당, 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 상술한 것 이외에 향미제로서 천연 향미제(예를 들어 레바우디오시드 A, 글리시르히진 등) 및 합성 향미제(사카린, 아스파르탐 등)를 유리하게 사용할 수 있다.Examples of the natural carbohydrate include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. In addition to the above, natural flavors (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can advantageously be used as flavoring agents.
상기 외에 본 발명의 건강기능식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 충진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다. 그밖에 천연 과일쥬스 및 과일 쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다.In addition to the above, the health functional food composition of the present invention is various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, it may contain fruit flesh for the manufacture of natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination.
본 발명에서 상기 건강보조식품은 건강기능식품 및 건강식품 등을 포함한다.In the present invention, the health supplement includes health functional food and health food.
상기 건강기능(성)식품(functional food)이란, 특정보건용 식품(food for special health use, FoSHU)과 동일한 용어로, 영양 공급 외에도 생체조절기능이 효율적으로 나타나도록 가공된 의학, 의료효과가 높은 식품을 의미한다. 여기서 "기능(성)"이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건용도에 유용한 효과를 얻는 것을 의미한다. 본 발명의 식품은 당 업계에서 통상적으로 사용되는 방법에 의하여 제조가능하며, 상기 제조시에는 당 업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있다. 또한 상기 식품의 제형 또한 식품으로 인정되는 제형이면 제한 없이 제조될 수 있다. 본 발명의 건강기능식품 조성물은 다양한 형태의 제형으로 제조될 수 있으며, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있다.The functional food is the same term as food for special health use (FoSHU), and is a medicine processed to efficiently display bioregulatory functions in addition to nutrient supply, and has high medical effect. means food. Here, "function (sex)" means to obtain useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions. The food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and ingredients commonly added in the art during the preparation. In addition, the formulation of the food may also be prepared without limitation as long as the formulation is recognized as a food. The health functional food composition of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time using food as a raw material.
본 발명의 방법에 있어서, 용어 "개체"는 임의의 동물 (예를 들어, 인간, 말, 돼지, 토끼, 개, 양, 염소, 비-인간 영장류, 소, 고양이, 기니피그 또는 설치류)을 포함하지만 이에 한정되지는 않는다. 이러한 용어는 특정 연령 또는 성별을 나타내지 않는다. 따라서, 여성/암컷이든 남성/수컷이든, 성인/성체 및 신생 대상체, 뿐만 아니라 태아가 포함되도록 의도된다. 환자는 질환 또는 장애에 걸린 대상체를 지칭한다. 환자라는 용어는 인간 및 수의학 대상체를 포함한다.In the methods of the present invention, the term "subject" includes any animal (eg, human, horse, pig, rabbit, dog, sheep, goat, non-human primate, cow, cat, guinea pig, or rodent), but It is not limited to this. These terms do not denote a specific age or gender. Thus, adult/adult and newborn subjects, whether female/female or male/male, as well as fetuses are intended to be included. A patient refers to a subject suffering from a disease or disorder. The term patient includes human and veterinary subjects.
본 발명의 방법에 있어서, 상기 화학식 1로 표시되는 아사로이독사진 B, 이의 이성질체, 이의 약학 또는 식품학적으로 허용 가능한 염 또는 세신 뿌리 분획물의 효과 및 이의 투여 경로, 투여 횟수, 투여량 등을 포함하는 구성에 대한 설명은 전술한 바와 동일하므로, 그 기재를 생략한다.In the method of the present invention, asaroidoxazine B represented by Formula 1, its isomer, its pharmaceutically or food-acceptable salt, or the effect of the root fraction and its administration route, including the number of administrations, dosage, etc. Since the description of the configuration is the same as described above, the description thereof is omitted.
본 발명의 제2 측면은 다음의 단계를 포함하는, 세신 (Asarum heterotropoides) 뿌리로부터 상기 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B)를 분리하는 방법에 관한 것이다:A second aspect of the present invention relates to a method for isolating Asaroidoxazine B represented by Formula 1 from Asarum heterotropoides roots, comprising the following steps:
(a) 세신 뿌리를 에탄올 용매로 추출하여 세신 뿌리 에탄올 추출물을 수득하는 단계;(a) extracting the sesame root with an ethanol solvent to obtain an ethanol extract of the sesame root;
(b) 상기 세신 뿌리 에탄올 추출물을 헥산, 에틸아세테이트 및 물 순서로 계통 분획하여 에틸아세테이트 분획물을 수득하는 단계;(b) obtaining an ethyl acetate fraction by systematically fractionating the ethanol extract of the sesame root in the order of hexane, ethyl acetate and water;
(c) 상기 에틸아세테이트 분획물을 물-메탄올 혼합액을 유출 용매로 하여 플래쉬 크로마토그래피를 수행하여 분획물을 수득하는 단계; 및(c) obtaining fractions by performing flash chromatography on the ethyl acetate fraction using a water-methanol mixture as an effluent solvent; and
(d) 플래쉬 크로마토그래피를 수행한 후 수득된 분획물을 물-아세토니트릴 혼합액을 유출 용매로 하여 반분취 크로마토그래피를 수행하여 상기 아사로이독사진 B (Asaroidoxazine B)를 정제하는 단계.(d) purifying the asaroidoxazine B by performing semipreparative chromatography on the fraction obtained after flash chromatography using a water-acetonitrile mixture as an effluent solvent.
본 발명의 방법에 있어서, 상기 (a) 단계의 세신 뿌리는 자연건조 또는 열풍건조 방법 등과 같이 통상적인 건조방법을 통해 건조한 것일 수 있다.In the method of the present invention, the thin roots of step (a) may be dried through a conventional drying method such as natural drying or hot air drying method.
본 발명의 방법에 있어서, 상기 (a) 단계에서 세신 뿌리를 추출하는데 사용되는 추출 용매의 종류는 에탄올이다. 에탄올을 이용한 추출 (즉, 주정추출)은 식품 가공상 가장 친환경적이며 경제적인 추출방법이다. 바람직하게는 50% 내지 90% 에탄올, 보다 바람직하게는 60% 내지 80% 에탄올, 가장 바람직하게는 65% 내지 75% 에탄올을 추출 용매를 사용할 수 있다.In the method of the present invention, in the step (a), the type of extraction solvent used to extract the roots of the shoot is ethanol. Ethanol extraction (i.e., alcohol extraction) is the most eco-friendly and economical extraction method in food processing. Preferably, 50% to 90% ethanol, more preferably 60% to 80% ethanol, and most preferably 65% to 75% ethanol may be used as the extraction solvent.
상기 추출물을 제조하는 방법은 특별히 제한되지 아니하며, 당해 기술분야에서 통상적으로 사용하는 방법에 따라 추출할 수 있다. 상기 추출 방법의 비제한적인 예로는, 침지 추출법, 열수 추출법, 초음파 추출법, 여과법, 환류 추출법 등을 들 수 있으며, 이들은 단독으로 수행되거나 2 종 이상의 방법을 병용하여 수행될 수 있다.A method for preparing the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include immersion extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, and the like, which may be performed alone or in combination of two or more methods.
본 발명의 방법에 있어서, 상기 (c) 단계는 물-메탄올 혼합액은 6:4에서 1:9의 농도 구배로 적용하여 플래쉬 크로마토그래피를 수행하는 것일 수 있으며, 바람직하게는 표준적 역상 소수성 수지, 예를 들어 C18 수지 (resin)로 충진된 컬럼을 이용하여 플래쉬 크로마토그래피로 수행될 수 있다.In the method of the present invention, step (c) may be to perform flash chromatography by applying a water-methanol mixture at a concentration gradient of 6:4 to 1:9, preferably a standard reversed-phase hydrophobic resin, For example, it can be performed by flash chromatography using a column packed with C18 resin.
본 발명의 방법에 있어서, 상기 (d) 단계의 물-아세토니트릴 혼합액은 6.5:3.5 내지 5.5:4.5의 농도 구배로 적용하여 반분취 크로마토그래피로 수행될 수 있으며, 바람직하게는 표준적 역상 소수성 수지, 예를 들어 ODS-A 수지 (resin)로 충진된 컬럼을 이용하여 반분취 크로마토그래피로 수행될 수 있다.In the method of the present invention, the water-acetonitrile mixture in step (d) may be applied with a concentration gradient of 6.5:3.5 to 5.5:4.5 to perform semi-preparative chromatography, preferably a standard reversed-phase hydrophobic resin. , for example, by semipreparative chromatography using a column packed with ODS-A resin.
이하, 하기 실시예에 의하여 본 발명을 보다 상세하게 설명한다. 단, 하기 실시예는 본 발명을 예시하기 위한 것일 뿐 본 발명의 범위가 이들로 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail by the following examples. However, the following examples are only for exemplifying the present invention, and the scope of the present invention is not limited thereto.
[실시예 1][Example 1]
세신 추출물 제조 및 아사로이독사진 B 화합물의 분리 Preparation of secin extract and isolation of asaroidoxazine B compound
1-1. 일반적인 절차1-1. general procedure
광학 회전 및 UV 스펙트럼은 각각 MCP-5500 디지털 편광계 (Anton Paar, Graz, Austria)와 Optizen POP 분광 광도계 (Mecasys, 대전, 대한민국)를 사용하여 결정되었다. 전자 원형 이색성 (electronic circular dichroism, ECD) 스펙트럼은 JASCO J-1100 분광편광계(Jasco, Tokyo, Japan)에서 기록되었다. NMR 스펙트럼은 AVANCE Ⅲ 600 MHz Cryo-NMR (Bruker, Billerica, MA, USA)에서 획득하였다. HRESIMS 데이터는 Bruker 컴팩트 또는 Waters Q-TOF 질량 분석기 (Waters, Milford, MA, USA)를 사용하여 획득하였다. 분취 HPLC는 YMC-Pack ODS-A 반분취 컬럼 (21.2 Х 250mm, 5μm, 120Å, YMC, Kyoto, Japan)이 구비된 2545Q 구배 모듈과 2998 PDA 검출기로 구성된 Waters prep-LC 시스템을 사용하여 수행하였다. 컬럼 크로마토그래피는 Biotage SNAP Ultra 100g 및 SNAP Ultra C18 120g 사전 팩킹 카트리지와 Sephadex LH-20 겔(MilliporeSigma, St. Louis, MO, USA)로 수동 팩킹된 Biotage SNAP 건조 로드 카트리지 10g이 구비된 Selekt 플래쉬 크로마토그래피 시스템(Biotage, Uppsala, Sweden)을 통해 수행되었다.Optical rotation and UV spectra were determined using an MCP-5500 digital polarimeter (Anton Paar, Graz, Austria) and an Optizen POP spectrophotometer (Mecasys, Daejeon, Korea), respectively. Electronic circular dichroism (ECD) spectra were recorded on a JASCO J-1100 spectropolarimeter (Jasco, Tokyo, Japan). NMR spectra were acquired on an AVANCE III 600 MHz Cryo-NMR (Bruker, Billerica, MA, USA). HRESIMS data were acquired using a Bruker compact or Waters Q-TOF mass spectrometer (Waters, Milford, MA, USA). Preparative HPLC was performed using a Waters prep-LC system consisting of a 2998 PDA detector and a 2545Q gradient module equipped with a YMC-Pack ODS-A semipreparative column (21.2 Х 250 mm, 5 μm, 120 Å, YMC, Kyoto, Japan). Column chromatography was performed using Selekt flash chromatography with Biotage SNAP Ultra 100 g and SNAP Ultra C18 120 g pre-packed cartridges and 10 g Biotage SNAP dry rod cartridges manually packed with Sephadex LH-20 gel (MilliporeSigma, St. Louis, MO, USA). system (Biotage, Uppsala, Sweden).
1-2. 추출 및 분리1-2. Extraction and Separation
건조된 세신 뿌리는 2019년에 KMD Medicinal Herbs Co. (울산, 대한민국)에서 구입하여 한국 한의학연구원 한약자원연구센터에 기탁하였다 (바우처 번호 2-19-0399).The dried seshin root was obtained from KMD Medicinal Herbs Co. in 2019. (Ulsan, South Korea) and deposited at the Herbal Medicine Resources Research Center of the Korea Institute of Oriental Medicine (voucher number 2-19-0399).
건조된 세신 뿌리 (1 kg)를 분쇄하고 40℃에서 침용하여 70% 에탄올 (10 L)로 추출한 다음 여과하고 진공에서 증발시켜 총 추출물 (86.3 g, 8.6%)을 수득하였다. 이 추출물 (80.0g)을 증류수 (1.5L)에 현탁하고 일련의 유기 용매로 분할하여 n-헥산-가용성 (9.3g), EtOAc-가용성 (2.7g) 및 수용성 추출물 (66.9g)을 수득하였다. 활성 EtOAc 추출물을 플래쉬 크로마토그래피 시스템 (Flash chromatography system, FCS)을 사용하여 2개의 SNAP Ultra C18 120g 카트리지 (물/MeOH, 6:4에서 1:9)로 분리하여 23개의 분획 (F01-F23)을 수득하였다. F14 (18.7 mg)를 YMC-Pack ODS-A 반분취 컬럼 (물/CH3CN, 6.5:3.5 내지 5.5:4.5, 50분)으로 정제하여 화합물 1 (4.9 mg)을 생성하였다.Dried seshin roots (1 kg) were ground and macerated at 40° C., extracted with 70% ethanol (10 L), filtered and evaporated in vacuo to give a total extract (86.3 g, 8.6%). This extract (80.0 g) was suspended in distilled water (1.5 L) and partitioned with a series of organic solvents to give n-hexane-soluble (9.3 g), EtOAc-soluble (2.7 g) and aqueous extracts (66.9 g). The active EtOAc extract was separated into two SNAP Ultra C18 120 g cartridges (water/MeOH, 6:4 to 1:9) using a flash chromatography system (FCS) to obtain 23 fractions (F01-F23). obtained. F14 (18.7 mg) was purified by YMC-Pack ODS-A semipreparative column (water/CH 3 CN, 6.5:3.5 to 5.5:4.5, 50 min) to give Compound 1 (4.9 mg).
아사로이독사진 B (Asaroidoxazine B) (화합물 1): 노란빛을 띤 결정; ECD (c 0.5 mM, CH3CN) Δε +3.97 (289), -5.44 (223); 1H (DMSO-d 6, 600 MHz) 및 13C (DMSO-d 6, 150 MHz) NMR 데이터는 표 1에 나타내었다. Asaroidoxazine B (Compound 1): yellowish crystals; ECD ( c 0.5 mM, CH 3 CN) Δε +3.97 (289), -5.44 (223); 1 H (DMSO- d 6 , 600 MHz) and 13 C (DMSO- d 6 , 150 MHz) NMR data are shown in Table 1.
positionposition 1One aa
δ C, type δC , type δ H, multi.
(J in Hz) δ H , multi.
( J in Hz)
1One 118.1, C118.1, C.
22 105.3, CH105.3, CH 7.60, s7.60, s
33 153.4, C153.4, C.
44 149.9, C149.9, C.
4a4a 112.9, C112.9, C.
4b4b 128.2, C128.2, C.
55 104.2, CH104.2, CH 7.45, s7.45, s
66 160.8, C160.8, C
77 99.5, CH99.5, CH 6.78, s6.78, s
88 157.5, C157.5, C.
8a8a 111.7, C111.7, C.
99 38.2, CH38.2, CH 5.31, s5.31, s
1010 151.7, C151.7, C.
10a10a 117.7, C117.7, C.
1111 162.8, C162.8, C
1212 104.0, CH2 104.0, CH2 6.44, s, 6.54, s6.44, s, 6.54, s
1′One' 34.4, CH2 34.4 CH 2 3.16, d (15.3), 3.29, d (15.3) 3.16, d (15.3), 3.29, d (15.3)
2′2' 169.5, C169.5, C
NH2-2′NH 2-2 ′ 7.11, br s, 7.32, br s7.11, brs, 7.32, brs
OCH3-6OCH 3-6 56.3, CH3 56.3 CH 3 3.94, s3.94, s
OCH3-7OCH 3 -7
OCH3-8OCH 3-8 55.5, CH3 55.5 CH 3 3.86, s3.86, s
aDMSO-d 6에서 측정됨. a DMSO-measured in d 6 .
1-3. 계산 방법 (Computational methods)1-3. Computational methods
형태 이성질체 (Conformer) 분포, 최적화 및 ECD 계산은 문헌 [Ryu, Seung Mok et al. "Chemical Constituents of the Egg Cases of　Tenodera angustipennis　(Mantidis ootheca) with Intracellular Reactive Oxygen Species Scavenging Activity."　Biomolecules　vol. 11,4 556. 10 Apr. 2021]에 기재된 바와 같이 수행되었다.Conformer distribution, optimization and ECD calculations are described by Ryu, Seung Mok et al. "Chemical Constituents of the Egg Cases of Tenodera angustipennis (Mantidis ootheca) with Intracellular Reactive Oxygen Species Scavenging Activity." Biomolecules vol. 11,4 556. 10 Apr. 2021].
1-4. X선 회절 분석1-4. X-ray diffraction analysis
CCD 데이터는 Bruker-SAINT 소프트웨어 패키지를 사용하여 통합 및 확장되었으며, SHEXL-2014를 사용하여 구조를 풀어 세밀하게 구분하였다. 화합물 1의 결정 데이터 - C20H16N2O7S(M = 428.41): 사방정계, 공간군 P212121(no. 19), a = 4.7058(6) Å, b = 19.547(3) Å, c = 19.654(3) Å, V = 1807.9(4) Å3, Z = 4, T = 100(2) K, μ(Cu-Kα) = 2.047 mm-1, Dcalc = 1.574 g/cm3, 3075 엑스선 반사 (3.189°≤ 2θ ≤ 69.920°), 3201 고유 엑스선 반사 (Rint = 0.1255)를 전체 계산에 사용하였다. 최종 R1은 0.0918 (I > 2σ(I))이었고, wR2는 0.1561 (I > 2σ(I))이었다. 화합물 1에 대한 결정학적 데이터는 캠브리지 결정학적 데이터 센터에 보충 간행물 번호 CCDC 2102594로 기탁되었다.CCD data were integrated and expanded using the Bruker-SAINT software package, and structured and refined using SHEXL-2014. Crystal data of compound 1 - C 2 0H 16 N 2 O 7 S (M = 428.41): orthorhombic, space group P212121 (no. 19), a = 4.7058(6) Å, b = 19.547(3) Å, c = 19.654(3) Å, V = 1807.9(4) Å3, Z = 4, T = 100(2) K, μ(Cu-Kα) = 2.047 mm -1 , D calc = 1.574 g/cm 3 , 3075 X-rays Reflection (3.189° ≤ 2θ ≤ 69.920°), 3201 intrinsic X-ray reflections (R int = 0.1255) were used for the overall calculation. The final R 1 was 0.0918 (I > 2σ(I)) and wR2 was 0.1561 (I > 2σ(I)). Crystallographic data for Compound 1 have been deposited with the Cambridge Crystallographic Data Center as Supplementary Publication No. CCDC 2102594.
화합물 1은 노란색 결정으로 수득되었으며, HRESIMS에서 451.0573 [M + Na]+에서 분자 이온을 관찰되었다. 화합물 1의 1D NMR 스펙트럼은 아사로이독사진 B의 스펙트럼과 유사하였다. 아사로이독사진 B는 옥사진고리를 포함하는 아리스토로크산 유도체로 최근 족도리풀속 (Asarum)에서 분리되는 것으로 보고되고 있다. 옥사진 고리의 존재는 NMR 데이터에서 확인하기 어렵다. 따라서, 화합물 1의 구조를 명확히 규명하기 위하여 X선 결정학을 이용하여 화합물 1의 구조를 추가로 확인하였다. 도 2의 X선 구조는 옥사진 고리의 존재를 분명히 보여주었다. 화합물 1의 C-9 위치에서의 절대 배열은 실험 및 계산된 ECD 스펙트럼 간의 비교를 통해 결정되었다. 도 3에서 확인되는 바와 같이, 실험적 ECD 스펙트럼은 9S 모델의 계산된 ECD 스펙트럼과 잘 일치하였다. 이러한 분광학적 증거에 기초하여, 화합물 1의 구조는 아사로이독사진 B로 밝혀졌다. Compound 1 was obtained as yellow crystals, and a molecular ion was observed at 451.0573 [M + Na] + in HRESIMS. The 1D NMR spectrum of compound 1 was similar to that of asaroidoxazine B. Asaroidoxazine B is an aristolochic acid derivative containing an oxazine ring and has recently been reported to be isolated from the genus Asarum . The presence of oxazine rings is difficult to confirm from NMR data. Therefore, in order to clarify the structure of Compound 1, the structure of Compound 1 was further confirmed using X-ray crystallography. The X-ray structure of Fig. 2 clearly showed the presence of oxazine rings. The absolute configuration at position C-9 of Compound 1 was determined through comparison between experimental and calculated ECD spectra. As confirmed in Fig. 3, the experimental ECD spectrum was in good agreement with the calculated ECD spectrum of the 9S model. Based on this spectroscopic evidence, the structure of compound 1 was determined to be asaroidoxazine B.
[실시예 2][Example 2]
MTT 어세이를 통한 항암 활성 확인Confirmation of anticancer activity through MTT assay
실시예 1에서 분리된 아사로이독사진 B의 항암 활성을 평가하기 위해, SKMEL5 (흑색종), A431 (비흑색종 피부), PC3 (전립선), A549 (폐), HCT116 (대장), AGS (위), MCF7 (유방암), K562 (백혈병), U-87 (신경교종), ACHN (신장샘암종) 및 Caki (투명세포 신장암)를 포함한 11개의 인간 암 세포주에 대한 세포독성 활성을 평가하였다.In order to evaluate the anticancer activity of asaroidoxazine B isolated in Example 1, SKMEL5 (melanoma), A431 (non-melanoma skin), PC3 (prostate), A549 (lung), HCT116 (colon), AGS (gastric) ), MCF7 (breast cancer), K562 (leukemia), U-87 (glioma), ACHN (renal adenocarcinoma) and Caki (clear cell renal cancer).
세포를 96-웰 플레이트에 씨딩 (웰당 1-2.5 x 103개 세포)한 다음 밤새 안정화시켰다. 그런 다음, 세포를 37℃, CO2 인큐베이터에서 24시간, 48시간 또는 72시간 동안 각각 다른 용량의 화합물로 처리하고 20㎕의 MTT ((4,5-디메틸티아졸-2-일)-2,5-디페닐테트라졸리움 브로마이드 시약(Sigma-Aldrich, Inc., St. Louis, MO, USA))을 각 웰에 첨가하였다. 1-2시간 후, 배지를 버리고 100μl의 DMSO (디메틸 설폭사이드, VWR Life Science, Ohio, USA)를 첨가한 다음 Multiskan Skyhigh 마이크로플레이트 분광광도계 (Thermo Scientific, Singapore)를 사용하여 570nm에서 흡광도를 측정하였다. 5-FU는 양성 대조군으로 사용하였다. 표 2의 추출물은 실시예 1-2의 세신 뿌리 분획물의 제조과정에서 수득된 70% 에탄올 세신 뿌리 추출물이다.Cells were seeded (1-2.5 x 10 3 cells per well) in 96-well plates and then allowed to settle overnight. Then, the cells were treated with different doses of the compounds for 24 hours, 48 hours or 72 hours in a 37° C. CO 2 incubator and 20 μl of MTT ((4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide reagent (Sigma-Aldrich, Inc., St. Louis, MO, USA) was added to each well. After 1-2 hours, the medium was discarded and 100 μl of DMSO (dimethyl sulfoxide, VWR Life Science, Ohio, USA) was added and absorbance was measured at 570 nm using a Multiskan Skyhigh microplate spectrophotometer (Thermo Scientific, Singapore). . 5-FU was used as a positive control. The extract in Table 2 is a 70% ethanol sessile root extract obtained in the process of preparing the sesshen root fraction of Example 1-2.
세포주cell line IC50 값 (72h)IC 50 value (72h)
아사로이독사진 B (nM) Asaroidoxin B (nM) 추출물 (ng/ml) Extract (ng/ml) 5-FU (μM) 5-FU (μM)
NHDF (정상 피부)NHDF (normal skin) n.d.n.d. n.d.n.d. n.d.n.d.
SKMEL5 (흑색종)SKMEL5 (melanoma) 398.2±5.5398.2±5.5 n.d.n.d. 8.4±0.78.4±0.7
A431 (비흑색종 피부암)A431 (non-melanoma skin cancer) 554.7±2.6554.7±2.6 n.d.n.d. 1.0±0.031.0±0.03
PC3 (전립선암)PC3 (prostate cancer) 690.9±6.3690.9±6.3 n.d.n.d. n.d.n.d.
A549 (폐암)A549 (lung cancer) 192.0±5.0192.0±5.0 n.d.n.d. 2.40±0.12.40±0.1
HCT116 (대장암)HCT116 (colorectal cancer) 244.3±3.5244.3±3.5 n.d.n.d. 3.5±0.13.5±0.1
AGS(위암)AGS (stomach cancer) 154.4±2.2154.4±2.2 n.d.n.d. 3.2±0.13.2±0.1
MCF7 (유방암)MCF7 (breast cancer) 375.3±5.8375.3±5.8 -- n.d.n.d.
K562 (백혈병)K562 (Leukemia) 424.8±7.5424.8±7.5 -- 6.7±0.26.7±0.2
U-87 (신경교종)U-87 (glioma) 755.2±23.5755.2±23.5 -- 9.0±0.29.0±0.2
ACHN (신장샘암종)ACHN (renal adenocarcinoma) 129.5±1.1129.5±1.1 -- 9.1±2.99.1±2.9
Caki (투명세포 신장암)Caki (clear cell kidney cancer) 669.0±5.2669.0±5.2 -- 9.9±0.29.9±0.2
n.d.: 검출되지 않음 (not detected)n.d.: not detected
그 결과, 표 2 및 도 4a 내지 4k에서 확인되는 바와 같이 아사로이독사진 B는 모든 암세포주에서 5-FU보다 훨씬 강한 나노몰 IC50 값을 갖는 강력한 억제 활성을 나타냈다.As a result, as confirmed in Table 2 and FIGS. 4a to 4k, asaroidoxazine B exhibited strong inhibitory activity with a much stronger nanomolar IC 50 value than 5-FU in all cancer cell lines.
구체적으로, 아사로이독사진 B는 시험한 모든 세포에 대해 129.5 내지 755.2 nM의 IC50 값으로 강력한 세포독성 활성을 나타냈다.Specifically, asaroidoxazine B exhibited potent cytotoxic activity with IC 50 values ranging from 129.5 to 755.2 nM against all cells tested.
[실시예 3][Example 3]
EGF-유도 발암 모델에서의 암 예방 효과 확인Confirmation of cancer prevention effect in EGF-induced carcinogenesis model
마우스 피부상피세포 JB6 (ATCC, 미국)에 암세포 발생을 증가시키는 성장인자인 EGF를 처리하여 악성 변형(malignant transformation)을 유도한 발암 모델에서 아사로이독사진 B의 암 유발 억제 효과를 확인하였다.In a carcinogenesis model in which malignant transformation was induced by treating mouse skin epithelial cell JB6 (ATCC, USA) with EGF, a growth factor that increases cancer cell development, the cancer-inducing inhibitory effect of asaroidoxazine B was confirmed.
구체적으로, BME, FBS, 글루타민, 젠타마이신, PBS 및 1.25% 아가가 포함된 아가 믹스 (0.5%)를 EGF (10ng/mL) 및 각 농도의 아사로이독사진 B와 섞어서 6 웰 플레이트에 웰 당 3ml씩 분주하여 굳혔다 (bottom agar). 마우스 피부상피세포 JB6(ATCC, USA)를 트립신을 처리하여 떼어낸 후, 1X PBS로 세척하고 10% BME로 재현탁하여 세포수를 확인하였다. 세포를 웰 당 8,000개씩 준비하고 아가 믹스 (0.3%), EGF, 및 농도별 아리스톨록사진 C (각각 0, 25, 50, 100 및 200 nM)와 섞어서 웰 당 1ml씩 분주하여 굳혔다 (upper agar). CO2 배양기에서 약 2주 동안 배양한 다음 현미경으로 콜로니 사진을 찍고, 콜로니 수를 Image-Pro Plus software (v.6.0) program (Media Cybernetics, Silver Spring, MD, USA)을 이용하여 계수하였다.Specifically, an agar mix (0.5%) containing BME, FBS, glutamine, gentamycin, PBS and 1.25% agar was mixed with EGF (10 ng/mL) and asaroidoxazine B at each concentration, 3ml per well in a 6-well plate. It was hardened by dividing it into portions (bottom agar). Mouse skin epithelial cells JB6 (ATCC, USA) were detached by treatment with trypsin, washed with 1X PBS, resuspended in 10% BME, and the number of cells was confirmed. 8,000 cells per well were prepared and mixed with agar mix (0.3%), EGF, and aristoloxazine C by concentration (0, 25, 50, 100 and 200 nM, respectively), and 1ml per well was dispensed and solidified (upper agar ). After culturing in a CO 2 incubator for about 2 weeks, colonies were photographed under a microscope and the number of colonies was counted using Image-Pro Plus software (v.6.0) program (Media Cybernetics, Silver Spring, MD, USA).
그 결과, 도 5에서 확인되는 바와 같이 같이 아사로이독사진 B가 처리된 시험군은 EGF-유도 발암 모델에서 EGF 처리군에 비해 31.1 내지 73.4%의 저해율을 나타내었고, 아사로이독사진 B의 농도 의존적으로 저해율이 증가됨을 확인하였다. 이를 통해, 아사로이독사진 B가 종양 발생을 억제 또는 지연시키는 효과가 있음을 확인하였다.As a result, as confirmed in FIG. 5, the test group treated with asaroidoxazine B exhibited an inhibition rate of 31.1 to 73.4% compared to the EGF-treated group in the EGF-induced carcinogenesis model, and asaroidoxazine B concentration-dependently. It was confirmed that the inhibition rate increased. Through this, it was confirmed that asaroidoxazine B has an effect of inhibiting or delaying tumor development.
이상의 설명으로부터, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시 예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로서 이해해야만 한다. 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (20)

  1. 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 약학적으로 허용 가능한 염을 유효성분으로 포함하는 암 예방 또는 치료용 약학 조성물:A pharmaceutical composition for preventing or treating cancer, comprising asaroidoxazine B represented by Formula 1, an isomer thereof or a pharmaceutically acceptable salt thereof as an active ingredient:
    [화학식 1][Formula 1]
    Figure PCTKR2022021008-appb-img-000003
    .
    Figure PCTKR2022021008-appb-img-000003
    .
  2. 제1항에 있어서, 상기 이성질체는 라세미체, 거울상 이성질체, 부분입체 이성질체, 거울상 이성질체의 혼합물 또는 부분입체 이성질체의 혼합물을 포함하는 것인, 암 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating cancer according to claim 1, wherein the isomer comprises a racemate, an enantiomer, a diastereomer, a mixture of enantiomers or a mixture of diastereomers.
  3. 제1항에 있어서, 상기 아사로이독사진 B는 세신 (Asarum heterotropoides) 뿌리로부터 분리되는 것인, 암 예방 또는 치료용 약학 조성물.According to claim 1, wherein the Asaroidoxazine B is seshin ( Asarum heterotropoides ) To be separated from the root, a pharmaceutical composition for preventing or treating cancer.
  4. 제1항에 있어서, 상기 아사로이독사진 B, 이의 이성질체 또는 약학적으로 허용 가능한 염은 암세포 억제 활성을 나타내는 것인, 암 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating cancer according to claim 1, wherein the asaroidoxazine B, an isomer or a pharmaceutically acceptable salt thereof exhibits cancer cell inhibitory activity.
  5. 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 식품학적으로 허용 가능한 염을 유효성분으로 포함하는 암 예방 또는 개선용 건강기능식품 조성물:Asaroidoxazine B represented by Formula 1, a health functional food composition for preventing or improving cancer comprising an isomer thereof or a food chemically acceptable salt as an active ingredient:
    [화학식 1][Formula 1]
    Figure PCTKR2022021008-appb-img-000004
    .
    Figure PCTKR2022021008-appb-img-000004
    .
  6. 제5항에 있어서, 상기 이성질체는 라세미체, 거울상 이성질체, 부분입체 이성질체, 거울상 이성질체의 혼합물 또는 부분입체 이성질체의 혼합물을 포함하는 것인, 암 예방 또는 개선용 건강기능식품 조성물.According to claim 5, wherein the isomer is a racemate, enantiomer, diastereomer, containing a mixture of enantiomers or a mixture of diastereomers, cancer preventing or improving functional health food composition.
  7. 제5항에 있어서, 상기 아사로이독사진 B는 세신 (Asarum heterotropoides) 뿌리로부터 분리되는 것인, 암 예방 또는 개선용 건강기능식품 조성물.According to claim 5, wherein the Asaroidoxazine B is seshin ( Asarum heterotropoides ) To be separated from the root, cancer prevention or improvement for health functional food composition.
  8. 제5항에 있어서, 상기 아사로이독사진 B, 이의 이성질체 또는 식품학적으로 허용 가능한 염은 암세포 억제 활성을 나타내는 것인, 암 예방 또는 개선용 건강기능식품 조성물.The functional food composition for preventing or improving cancer according to claim 5, wherein the asaroidoxazine B, an isomer thereof or a food-acceptable salt exhibits cancer cell inhibitory activity.
  9. 다음의 단계를 포함하는, 세신 (Asarum heterotropoides) 뿌리로부터 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine C)를 분리하는 방법:A method for isolating Asaroidoxazine C represented by Formula 1 from Asarum heterotropoides roots, including the following steps:
    [화학식 1][Formula 1]
    Figure PCTKR2022021008-appb-img-000005
    Figure PCTKR2022021008-appb-img-000005
    (a) 세신 뿌리를 에탄올 용매로 추출하여 세신 뿌리 에탄올 추출물을 수득하는 단계;(a) extracting the sesame root with an ethanol solvent to obtain an ethanol extract of the sesshen root;
    (b) 상기 세신 뿌리 에탄올 추출물을 헥산, 에틸아세테이트 및 물 순서로 계통 분획하여 에틸아세테이트 분획물을 수득하는 단계;(b) obtaining an ethyl acetate fraction by systematically fractionating the ethanol extract of the sesame root in the order of hexane, ethyl acetate and water;
    (c) 상기 에틸아세테이트 분획물을 물-메탄올 혼합액을 유출 용매로 하여 플래쉬 크로마토그래피를 수행하여 분획물을 수득하는 단계; 및(c) obtaining fractions by performing flash chromatography on the ethyl acetate fraction using a water-methanol mixture as an effluent solvent; and
    (d) 컬럼 크로마토그래피를 수행한 후 수득된 분획물을 물-아세토니트릴 혼합액을 유출 용매로 하여 반분취 크로마토그래피를 수행하여 상기 아사로이독사진 B (Asaroidoxazine B)를 정제하는 단계.(d) purifying the asaroidoxazine B by performing semipreparative chromatography on the fraction obtained after column chromatography using a water-acetonitrile mixture as an effluent solvent.
  10. 제9항에 있어서, 상기 (a) 단계의 에탄올 용매는 50 내지 90% 에탄올 용매인 것인, 방법.The method of claim 9, wherein the ethanol solvent in step (a) is a 50 to 90% ethanol solvent.
  11. 제9항에 있어서, 상기 (c) 단계의 물-메탄올 혼합액은 6:4에서 1:9의 농도 구배로 적용되는 것인, 방법.The method of claim 9, wherein the water-methanol mixture of step (c) is applied with a concentration gradient of 6:4 to 1:9.
  12. 제9항에 있어서, 상기 (d) 단계의 물-아세토니트릴 혼합액은 6.5:3.5 내지 5.5:4.5의 농도 구배로 적용되는 것인, 방법.The method of claim 9, wherein the water-acetonitrile mixture of step (d) is applied with a concentration gradient of 6.5:3.5 to 5.5:4.5.
  13. 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 약학적으로 허용 가능한 염을 이를 필요로 하는 개체에게 투여하는 것을 포함하는, 암 예방 또는 치료 방법:A method for preventing or treating cancer, comprising administering Asaroidoxazine B represented by Formula 1, an isomer thereof or a pharmaceutically acceptable salt thereof to a subject in need thereof:
    [화학식 1][Formula 1]
    Figure PCTKR2022021008-appb-img-000006
    .
    Figure PCTKR2022021008-appb-img-000006
    .
  14. 제13항에 있어서, 상기 이성질체는 라세미체, 거울상 이성질체, 부분입체 이성질체, 거울상 이성질체의 혼합물 또는 부분입체 이성질체의 혼합물을 포함하는 것인, 방법.14. The method of claim 13, wherein the isomers include racemates, enantiomers, diastereomers, mixtures of enantiomers or mixtures of diastereomers.
  15. 제13항에 있어서, 상기 아사로이독사진 B는 세신 (Asarum heterotropoides) 뿌리로부터 분리되는 것인, 방법.The method of claim 13, wherein the Asaroidoxazine B is separated from the roots of Asarum heterotropoides .
  16. 제13항에 있어서, 상기 아사로이독사진 B, 이의 이성질체 또는 약학적으로 허용 가능한 염은 암세포를 억제하거나, 종양 발생을 억제 또는 지연시키는 효과를 나타내는 것인, 암 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating cancer according to claim 13, wherein the asaroidoxazine B, an isomer thereof or a pharmaceutically acceptable salt exhibits an effect of suppressing cancer cells or inhibiting or delaying tumor development.
  17. 암의 예방, 개선 또는 치료를 위한 약제 또는 건강기능식품의 제조 시 화학식 1로 표시되는 아사로이독사진 B (Asaroidoxazine B), 이의 이성질체 또는 이의 약학 또는 식품학적으로 허용 가능한 염의 용도:Use of Asaroidoxazine B represented by Formula 1, its isomer or its pharmaceutically or food-acceptable salt in the preparation of pharmaceuticals or health functional foods for the prevention, improvement or treatment of cancer:
    [화학식 1][Formula 1]
    Figure PCTKR2022021008-appb-img-000007
    .
    Figure PCTKR2022021008-appb-img-000007
    .
  18. 제17항에 있어서, 상기 이성질체는 라세미체, 거울상 이성질체, 부분입체 이성질체, 거울상 이성질체의 혼합물 또는 부분입체 이성질체의 혼합물을 포함하는 것인, 용도.18. Use according to claim 17, wherein said isomers include racemates, enantiomers, diastereomers, mixtures of enantiomers or mixtures of diastereomers.
  19. 제17항에 있어서, 상기 아사로이독사진 B는 세신 (Asarum heterotropoides) 뿌리로부터 분리되는 것인, 용도.The use according to claim 17, wherein the asaroidoxazine B is isolated from the roots of Asarum heterotropoides .
  20. 제17항에 있어서, 상기 아사로이독사진 B, 이의 이성질체 또는 이의 약학 또는 식품학적으로 허용 가능한 염은 암세포를 억제하거나, 종양 발생을 억제 또는 지연시키는 효과를 나타내는 것인, 용도.The use according to claim 17, wherein the asaroidoxazine B, an isomer thereof, or a pharmaceutically or food-acceptable salt thereof exhibits an effect of inhibiting cancer cells or suppressing or delaying tumor development.
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