WO2023108017A3 - Carbon sources for improved microbial cellulose biosynthesis - Google Patents

Carbon sources for improved microbial cellulose biosynthesis Download PDF

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Publication number
WO2023108017A3
WO2023108017A3 PCT/US2022/081108 US2022081108W WO2023108017A3 WO 2023108017 A3 WO2023108017 A3 WO 2023108017A3 US 2022081108 W US2022081108 W US 2022081108W WO 2023108017 A3 WO2023108017 A3 WO 2023108017A3
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WO
WIPO (PCT)
Prior art keywords
carbon sources
microbial cellulose
cellulose biosynthesis
improved microbial
bacteria
Prior art date
Application number
PCT/US2022/081108
Other languages
French (fr)
Other versions
WO2023108017A2 (en
Inventor
Helen H. Lu
Theanne SCHIROS
Yueh-Ting CHIU
Romare M. ANTROBUS
Original Assignee
The Trustees Of Columbia University In The City Of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Trustees Of Columbia University In The City Of New York filed Critical The Trustees Of Columbia University In The City Of New York
Publication of WO2023108017A2 publication Critical patent/WO2023108017A2/en
Publication of WO2023108017A3 publication Critical patent/WO2023108017A3/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

A method of producing microbial biocellulose from a bacteria can comprise pre-culturing at least one bacteria in Hestrin-Schramm (HS) medium at a temperature of 20-40 °C for 50-80 hours under a static condition, and inoculating at least one carbon source at a concentration between 1% and 15% with pre-cultured bacteria obtained in step (a) at a temperature of 20-40 °C for 10-20 days under static condition.
PCT/US2022/081108 2021-12-07 2022-12-07 Carbon sources for improved microbial cellulose biosynthesis WO2023108017A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163286953P 2021-12-07 2021-12-07
US63/286,953 2021-12-07

Publications (2)

Publication Number Publication Date
WO2023108017A2 WO2023108017A2 (en) 2023-06-15
WO2023108017A3 true WO2023108017A3 (en) 2023-07-06

Family

ID=86731385

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/081108 WO2023108017A2 (en) 2021-12-07 2022-12-07 Carbon sources for improved microbial cellulose biosynthesis

Country Status (1)

Country Link
WO (1) WO2023108017A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170145469A1 (en) * 2015-11-19 2017-05-25 Samsung Electronics Co., Ltd. Method of screening for microorganism having enhanced cellulose productivity
US20170153541A1 (en) * 2015-11-27 2017-06-01 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Pattern definition of nanocellulose sheets through selective ashing via lithographic masking.
US20210115483A1 (en) * 2019-10-17 2021-04-22 Worcester Polytechnic Institute Transparent Cellulose-Based Materials and Methods of Making the Same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170145469A1 (en) * 2015-11-19 2017-05-25 Samsung Electronics Co., Ltd. Method of screening for microorganism having enhanced cellulose productivity
US20170153541A1 (en) * 2015-11-27 2017-06-01 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Pattern definition of nanocellulose sheets through selective ashing via lithographic masking.
US20210115483A1 (en) * 2019-10-17 2021-04-22 Worcester Polytechnic Institute Transparent Cellulose-Based Materials and Methods of Making the Same

Also Published As

Publication number Publication date
WO2023108017A2 (en) 2023-06-15

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