Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
  • C12N15/09—Recombinant DNA-technology
  • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
  • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
  • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
  • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
  • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
  • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
  • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
  • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
  • A01H5/08—Fruits
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
  • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
  • A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
  • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
  • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
  • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
  • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
  • A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
  • A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
  • A01H1/1255—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for fungal resistance
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
  • A01H5/10—Seeds
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
  • A01H6/34—Cucurbitaceae, e.g. bitter melon, cucumber or watermelon 
  • A01H6/348—Cucurbita, e.g. squash or pumpkin
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
  • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
  • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
  • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q2600/00—Oligonucleotides characterized by their use
  • C12Q2600/13—Plant traits
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q2600/00—Oligonucleotides characterized by their use
  • C12Q2600/156—Polymorphic or mutational markers

Definitions

• Cucurbita pepo L. is an important specialty crop native of North America.
• the cultivated, edible forms of Cucurbita are a common crop in many major agriculture production areas and represented a world production of 22,900,826 tonnes in 2019 (derived from data supplied by the Food and Agriculture Organization).
• the United States production alone was worth up to 220 million of US dollars in 2019 (USDA Vegetables 2019 Summary).
• cylindrica plant resistant to downy mildew infection comprising the steps of a) crossing a plant according to any one of the preceding embodiments with a cultivated squash plant lacking said downy mildew resistance-conferring introgressed sequence; b) selecting a progeny plant comprising said introgressed sequence located on chromosome 9 conferring resistance to downy mildew, said selecting step comprising detecting at least one of the following SNP markers: i) a G genotype in the heterozygous or homozygous state for SNP marker 1 at a position corresponding to position 36 in SEQ ID NO: 1 ; ii) a G genotype in the heterozygous or homozygous state for SNP marker
• genetically fixed refers to a genetic sequence which has been stably incorporated into the genome of a plant that normally does not contain said genetic sequence. When genetically fixed, the genetic sequence can be transmitted in an easy and predictable manner to other plants by sexual crosses.
• PCR Polymerase chain reaction
• PCR primer is understood within the scope of the invention to refer to relatively short fragments of single-stranded DNA used in the PCR amplification of specific regions of DNA.
• Probe refers to a group of atoms or molecules which can recognise and bind to a specific target molecule or cellular structure and thus allowing detection of the target molecule or structure. Particularly, “probe” refers to a labelled DNA or RNA sequence which can be used to detect the presence of and to quantitate a complementary sequence by molecular hybridization.
• said plant is heterozygous for said at least one SNP marker. In a further embodiment of the invention, said plant is homozygous for said at least one SNP marker.
• the invention provides a seed that produces a plant according to any of the preceding embodiments.
• the invention relates to the use of a squash plant according to any of the preceding embodiments as a rootstock, preferably a cucurbit rootstock, more preferably a squash rootstock.
• the invention relates to the use of Cucurbita pepo plant 21 SQN 100201 , representative seed of which is deposited under ATCC Accession No. PTA-127157, or a progeny or an ancestor thereof, as a squash rootstock.
• the invention in another embodiment relates to a method of identifying a squash plant comprising the downy mildew resistance-conferring introgressed sequence of the invention, wherein said method comprises the steps of: a) providing a population segregating for the downy mildew resistance trait, b) screening the segregating population for a member exhibiting resistance to downy mildew, wherein said trait can be identified by the presence of downy mildew resistance-conferring introgressed sequence of the invention, c) selecting one member of the segregating population, wherein said member comprises the downy mildew resistance trait.
• the invention provides a method for identifying a cultivated squash plant comprising an introgressed sequence on chromosome 9, wherein said introgressed sequence confers resistance to downy mildew, comprising: a) providing a population segregating for downy mildew resistance, b) screening said population using a kit which detects at least one of the SNP markers listed in Table 4, and, c) identifying a plant comprising said at least one SNP marker selected in the list of Table 4.
• cylindrica plant an introgressed sequence associated with an increased resistance to downy mildew
• said method comprising the step of detecting in said plant an allele of at least one DNA marker that is genetically linked to a QTL locus associated with said increased resistance to downy mildew, wherein said allele maps within 10cM, preferably within 5cM of said QTL locus located on chromosome 9 in a genomic region flanked by SNP markers 1 and 8.
• the present invention also relates to the use of downy mildew resistance-propagating material obtainable from a squash plant according to any of the preceding embodiments for growing a squash plant to produce downy mildew resistant squash plants wherein said downy mildew resistance may be assessed in a standard assay, particularly an assay as described in Example 2 below.
• R number of plants with a score equal to 9;
• Table 1 Significant QTL associated with downy mildew resistance.

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• Genetics & Genomics (AREA)
• Botany (AREA)
• Developmental Biology & Embryology (AREA)
• Environmental Sciences (AREA)
• Chemical & Material Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Engineering & Computer Science (AREA)
• Organic Chemistry (AREA)
• Zoology (AREA)
• Biotechnology (AREA)
• Wood Science & Technology (AREA)
• Physics & Mathematics (AREA)
• Physiology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Molecular Biology (AREA)
• General Engineering & Computer Science (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Analytical Chemistry (AREA)
• Biomedical Technology (AREA)
• Biophysics (AREA)
• Biochemistry (AREA)
• Microbiology (AREA)
• Natural Medicines & Medicinal Plants (AREA)
• Spectroscopy & Molecular Physics (AREA)
• Plant Pathology (AREA)
• Cell Biology (AREA)
• Immunology (AREA)
• Mycology (AREA)
• Animal Husbandry (AREA)
• Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
• Pretreatment Of Seeds And Plants (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)

Priority Applications (9)

Applications Claiming Priority (2)

Publications (1)

Family

ID=82323838

Family Applications (1)

Country Status (10)

Families Citing this family (1)

Family Cites Families (2)

• 2021
  • 2021-12-01 US US17/539,392 patent/US11384362B1/en active Active
• 2022
  • 2022-11-30 AU AU2022399921A patent/AU2022399921A1/en active Pending
  • 2022-11-30 KR KR1020247021037A patent/KR20240112909A/ko active Pending
  • 2022-11-30 WO PCT/EP2022/083878 patent/WO2023099576A1/en not_active Ceased
  • 2022-11-30 JP JP2024532259A patent/JP2024544064A/ja active Pending
  • 2022-11-30 EP EP22830689.0A patent/EP4440301A1/en active Pending
  • 2022-11-30 IL IL312686A patent/IL312686A/en unknown
  • 2022-11-30 CA CA3238354A patent/CA3238354A1/en active Pending
  • 2022-11-30 MX MX2024006644A patent/MX2024006644A/es unknown
  • 2022-11-30 US US18/711,658 patent/US20250008902A1/en active Pending
  • 2022-11-30 MA MA65763A patent/MA65763A1/fr unknown

Non-Patent Citations (10)

Also Published As

Similar Documents

Legal Events