WO2023077029A3 - Single cell viral integration site detection - Google Patents

Single cell viral integration site detection Download PDF

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Publication number
WO2023077029A3
WO2023077029A3 PCT/US2022/078821 US2022078821W WO2023077029A3 WO 2023077029 A3 WO2023077029 A3 WO 2023077029A3 US 2022078821 W US2022078821 W US 2022078821W WO 2023077029 A3 WO2023077029 A3 WO 2023077029A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
integration site
segment
single cell
genomic
Prior art date
Application number
PCT/US2022/078821
Other languages
French (fr)
Other versions
WO2023077029A2 (en
WO2023077029A9 (en
Inventor
Dalia Dhingra
Adam SCIAMBI
Chieh-Yuan Li
Original Assignee
Mission Bio, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mission Bio, Inc. filed Critical Mission Bio, Inc.
Publication of WO2023077029A2 publication Critical patent/WO2023077029A2/en
Publication of WO2023077029A3 publication Critical patent/WO2023077029A3/en
Publication of WO2023077029A9 publication Critical patent/WO2023077029A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides single-cell analysis using combined DNA sequencing of genomic DNA, which is performed to determine whether a segment of foreign DNA has been integrated. Individual cells are encapsulated with reagents, including primers, and lysed. Cell lysates include genomic DNA and the genomic DNA contains an integration site including a foreign DNA segment, if present. Segments of DNA are primed, amplified, and sequenced to generate sequence reads of the genomic DNA. Sequence reads from the DNA-seq reveal the presence or absence of a foreign DNA segment, and the genomic locus of the integration site, if present.
PCT/US2022/078821 2021-10-27 2022-10-27 Single cell viral integration site detection WO2023077029A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US202163272649P 2021-10-27 2021-10-27
US63/272,649 2021-10-27
US202263407593P 2022-09-16 2022-09-16
US63/407,593 2022-09-16
US202263416766P 2022-10-17 2022-10-17
US63/416,766 2022-10-17

Publications (3)

Publication Number Publication Date
WO2023077029A2 WO2023077029A2 (en) 2023-05-04
WO2023077029A3 true WO2023077029A3 (en) 2023-08-31
WO2023077029A9 WO2023077029A9 (en) 2024-03-14

Family

ID=86160664

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/078821 WO2023077029A2 (en) 2021-10-27 2022-10-27 Single cell viral integration site detection

Country Status (1)

Country Link
WO (1) WO2023077029A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200325471A1 (en) * 2016-07-12 2020-10-15 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
US20210230671A1 (en) * 2018-08-09 2021-07-29 Juno Therapeutics, Inc. Methods for assessing integrated nucleic acids
WO2021188889A1 (en) * 2020-03-20 2021-09-23 Mission Bio, Inc. Single cell workflow for whole genome amplification
WO2021202403A1 (en) * 2020-03-30 2021-10-07 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200325471A1 (en) * 2016-07-12 2020-10-15 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
US20210230671A1 (en) * 2018-08-09 2021-07-29 Juno Therapeutics, Inc. Methods for assessing integrated nucleic acids
WO2021188889A1 (en) * 2020-03-20 2021-09-23 Mission Bio, Inc. Single cell workflow for whole genome amplification
WO2021202403A1 (en) * 2020-03-30 2021-10-07 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries

Also Published As

Publication number Publication date
WO2023077029A2 (en) 2023-05-04
WO2023077029A9 (en) 2024-03-14

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