WO2023075721A1 - Lame de microscope numérique pour améliorer les compétences d'utilisation d'un microscope - Google Patents

Lame de microscope numérique pour améliorer les compétences d'utilisation d'un microscope Download PDF

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Publication number
WO2023075721A1
WO2023075721A1 PCT/TR2021/051314 TR2021051314W WO2023075721A1 WO 2023075721 A1 WO2023075721 A1 WO 2023075721A1 TR 2021051314 W TR2021051314 W TR 2021051314W WO 2023075721 A1 WO2023075721 A1 WO 2023075721A1
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WO
WIPO (PCT)
Prior art keywords
image
software
organelles
microscope
improve
Prior art date
Application number
PCT/TR2021/051314
Other languages
English (en)
Inventor
Fatih TAS
Original Assignee
Tas Fatih
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tas Fatih filed Critical Tas Fatih
Priority to DE112021004769.8T priority Critical patent/DE112021004769T5/de
Publication of WO2023075721A1 publication Critical patent/WO2023075721A1/fr

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/368—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements details of associated display arrangements, e.g. mounting of LCD monitor

Definitions

  • the invention relates to a digital slide system, which eliminates the need for physical use of slide and the need for sample tissue to be examined together with the slide, in order to provide convenience for those who need to receive microscope usage training.
  • microscopes are a type of conventional optical microscope that uses a variety of optics and a digital camera to transfer the image to the monitor, usually by means of software running through a computer.
  • a digital microscope usually has an integrated LED light source and differs from an optical microscope as there is no reason to observe the sample directly with the eyepiece.
  • the entire system is designed for monitor display, as the image is focused digitally.
  • Optics specific to the human eye have been removed from the system.
  • Ready-made preparations are used in these microscopes, as in conventional microscopes, and their images are projected onto the screen. However, in this method, it is not possible to gain the ability to use a microscope. Because there is no visual examination, the examination is carried out directly on the screen.
  • FIG. 1 Upper View of the Bottom Part and the Directing Panel Figure 5. Upper View Showing the Scroll Axis Contacting the Directing Panel by Moving the Scroll Axis to the Right
  • FIG. 1 Showing the Scroll Axis Contacting the Directing Panel by Moving the Scroll Axis to the Left
  • the invention is about a system designed for users who will receive microscope training comprising an data storage area where images can be loaded, a software that can detect graphics showing intracellular organelles and formations in the image by processing the uploaded images, apply animations and effects so that these organelles and formations can be seen as living cell tissue, a digital display (4) that provides imaging so that the processed images can be viewed through a microscope and a scroll axis (2) on its four sides, which enables the image to be shifted, thus provides a feeling of using a real microscope slide.
  • the first problem is the necessity of preparing a sample for each tissue. Thanks to the invention, all images are uploaded digitally with a single digital slide set, enabling the trainee to work on the desired image. In addition, the obligations related to the proper collection of the sample to be examined will also be eliminated. As a matter of fact, it is not possible to reach the desired training result by viewing a sample that is not taken in the right standards. In our invention, the whole process is completed by only uploading the desired image to the system. The remaining operations are carried out by the software included in the system, and the examination of a real texture image is simulated with the effects to be applied to the loaded image.
  • the images uploaded to the storage area of the system subject to the invention are analyzed by software. This analysis is aimed at determining the shapes of intracellular organelles and formations in the image that have been previously defined to the software.
  • the shapes of all intracellular organelles and formations are defined in the software such as, the cell nucleus is in a circular structure or the mitochondria is in a bean-like elliptical structure. Although these definitions cover all kinds of organelles and formations in human, animal and plant cells that can be examined, the user can add to the definitions if desired. Since the defined shapes are known by the software, a scan is performed to see if these shapes exist in the image uploaded to the system for review by the user. For scanning, first filtering and noise removal steps are applied to the image.
  • the median filter which is applied to reduce the effects of deformation and noise, gives good results especially in images with random noise distribution.
  • This filter was chosen because of its good noise reduction capability and less blurring than smaller sized linear smoothing filters.
  • a background removal process is applied to the image. In this step, the parts that do not have any patterns on them are detected and deleted by the software.
  • the Sobel operator is applied to the image with the background removed and the edges of the objects are determined. Corner, arc and straight-line geometric features are searched on this image in which the edges are detected. Names are determined by matching the organelles and formations formed by these searched and detected lines with the organelles and formations uploaded to the database. The routine movements of organelles and formations, whose names are known, are also recorded in the system database.
  • Movements are applied to the detected organelles as they are uploaded to the database. Movements can be varied as point-to-point movement, circular movements, trembling, back-and-forth movement, or staying still. In this way, organelles and formations are detected on a fixed cell image and the ability to move in accordance with its original is gained. The gaps of the background detected in the previous steps are filled by copying the images in the nearest pixels during this movement. Thus, the background deficiency is never seen by the user.
  • the display (4) in the system subject to the invention displays the image processed and made ready for education by the software.
  • the image projected by the display (4) is ready to be viewed with the microscope.
  • the image that has been finalized with the software and is ready to experience the experience that would be obtained with a classical microscope image can be seen.
  • the microscope slide In classical microscopes, the microscope slide is moved in order to move the parts of the image placed between the slide and the coverslip that come under the lens of the microscope. Thus, the image under the lens is transmitted to the eye of the user.
  • a scroll axis (2) in order to have this experience.
  • the scroll axis (2) positioned between the upper body (1 ) and the lower body (3) of the digital slide training set is fixed with guide gaps so that it is activated on a fixed line when it is pulled down, up, right or left.
  • the protrusions on the upper body (1 ) and the lower body (3) coincide with the guide spaces on the scroll axis (2) and thus enable the scroll axis (2) to move on a fixed line.
  • a direction button (6) located on the left side on the middle section (5).
  • a function of shifting the image to the right is defined for this button.
  • a direction button (6) is placed on the opposite side of each direction that is desired to be shifted, with the command to shift the image in the relevant direction.
  • a command is transmitted to the software to scroll the image by touching the direction buttons (6).
  • Four direction buttons (6) are positioned on the four lateral sides of the middle section (5), one on each side.
  • the invention comprises a data transfer port that allows the user to upload the images to the storage area.
  • This data transfer port is any of the USB connection types.
  • the software automatically sees the connection and shows the image files in the connected media on the display (4) via the interface.
  • the scroll axis (2) the user can switch between the files that appear on the display (4).
  • the cursor is on the image to be transferred, the image can be copied to the storage area by pressing any two direction buttons (6) together.
  • the inside of the storage area is displayed via the display (4).
  • the system As soon as the system, which is the subject of the invention, is turned on, it displays a main window in which the recorded images are displayed. In this window, the image to be made ready from the recorded images is selected by moving via the scroll axis (2) and pressing any two direction buttons (6) at the same time. Thus, the invention makes the image ready by applying the abovedescribed steps.
  • the prepared image is visible under a microscope.
  • the cursor is on an image, if any three direction buttons (6) are pressed, the command to delete the file is sent to the software. Thus, a recorded image can be deleted. If four direction buttons (6) are pressed together for more than two seconds, the system shuts down. All these functions build up the user interface within the software.

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Processing Or Creating Images (AREA)
  • Microscoopes, Condenser (AREA)
  • Image Processing (AREA)

Abstract

L'invention concerne un système de lame numérique, ce qui élimine le besoin d'utilisation physique de lame et la nécessité d'analyser un tissu d'échantillon conjointement avec la lame, afin de fournir une commodité pour ceux qui ont besoin de recevoir un apprentissage d'utilisation de microscope.
PCT/TR2021/051314 2021-10-29 2021-11-30 Lame de microscope numérique pour améliorer les compétences d'utilisation d'un microscope WO2023075721A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112021004769.8T DE112021004769T5 (de) 2021-10-29 2021-11-30 Digitaler Mikroskop-Objektträger zur Verbesserung der Mikroskop-Nutzungsfähigkeiten

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2021/016867 2021-10-29
TR2021/016867A TR2021016867A2 (tr) 2021-10-29 2021-10-29 Mi̇kroskop kullanim beceri̇si̇ni̇ geli̇şti̇rmek amaçli di̇ji̇tal lam-lamel

Publications (1)

Publication Number Publication Date
WO2023075721A1 true WO2023075721A1 (fr) 2023-05-04

Family

ID=85113175

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2021/051314 WO2023075721A1 (fr) 2021-10-29 2021-11-30 Lame de microscope numérique pour améliorer les compétences d'utilisation d'un microscope

Country Status (3)

Country Link
DE (1) DE112021004769T5 (fr)
TR (1) TR2021016867A2 (fr)
WO (1) WO2023075721A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1324097A2 (fr) * 2001-12-18 2003-07-02 Fairfield Imaging Ltd. Méthode et appareil pour détecter des photos digitales d'un microscope
US20060228107A1 (en) * 2005-04-11 2006-10-12 Direct Communications, Inc. Slide photograph data creation system, and slide photograph data
US20170329121A1 (en) * 2016-05-12 2017-11-16 Optrascan, Inc. Smart Microscope Slide for Concurrent Storage of a Specimen and Associated Digital Data, and Method of Use Thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1324097A2 (fr) * 2001-12-18 2003-07-02 Fairfield Imaging Ltd. Méthode et appareil pour détecter des photos digitales d'un microscope
US20060228107A1 (en) * 2005-04-11 2006-10-12 Direct Communications, Inc. Slide photograph data creation system, and slide photograph data
US20170329121A1 (en) * 2016-05-12 2017-11-16 Optrascan, Inc. Smart Microscope Slide for Concurrent Storage of a Specimen and Associated Digital Data, and Method of Use Thereof

Also Published As

Publication number Publication date
DE112021004769T5 (de) 2023-07-06
TR2021016867A2 (tr) 2021-11-22

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