WO2023049380A3 - Compositions and methods for overproduction of homogentisic acid and pyomelanin isoforms in yeast - Google Patents

Compositions and methods for overproduction of homogentisic acid and pyomelanin isoforms in yeast Download PDF

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Publication number
WO2023049380A3
WO2023049380A3 PCT/US2022/044584 US2022044584W WO2023049380A3 WO 2023049380 A3 WO2023049380 A3 WO 2023049380A3 US 2022044584 W US2022044584 W US 2022044584W WO 2023049380 A3 WO2023049380 A3 WO 2023049380A3
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WO
WIPO (PCT)
Prior art keywords
pyomelanin
methods
homogentisic acid
overproduction
isoforms
Prior art date
Application number
PCT/US2022/044584
Other languages
French (fr)
Other versions
WO2023049380A2 (en
Inventor
Hal S. Alper
Kelly K. MILLER
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Board Of Regents, The University Of Texas System
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Application filed by Board Of Regents, The University Of Texas System filed Critical Board Of Regents, The University Of Texas System
Publication of WO2023049380A2 publication Critical patent/WO2023049380A2/en
Publication of WO2023049380A3 publication Critical patent/WO2023049380A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11027—4-Hydroxyphenylpyruvate dioxygenase (1.13.11.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14009—4-Hydroxyphenylacetate 3-monooxygenase (1.14.14.9)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are genetically modified yeast cells engineered for increased production amounts and rates of biosynthetic homogentisic acid and biosynthetic pyomelanin. Also provided are methods using the disclosed genetically modified yeast cells, biosynthetic homogentisic acid, and biosynthetic pyomelanin.
PCT/US2022/044584 2021-09-24 2022-09-23 Compositions and methods for overproduction of homogentisic acid and pyomelanin isoforms in yeast WO2023049380A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163248217P 2021-09-24 2021-09-24
US63/248,217 2021-09-24

Publications (2)

Publication Number Publication Date
WO2023049380A2 WO2023049380A2 (en) 2023-03-30
WO2023049380A3 true WO2023049380A3 (en) 2023-11-09

Family

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Family Applications (1)

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PCT/US2022/044584 WO2023049380A2 (en) 2021-09-24 2022-09-23 Compositions and methods for overproduction of homogentisic acid and pyomelanin isoforms in yeast

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WO (1) WO2023049380A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846531A (en) * 1990-03-21 1998-12-08 University Of Maryland Marine mela gene
US20190058193A1 (en) * 2015-10-09 2019-02-21 River Road Research, Inc. Method for producing lithium-ion, sodium-ion and potassium-ion batteries with increased safety
US20210180092A1 (en) * 2017-11-06 2021-06-17 Toyota Jidosha Kabushiki Kaisha Mutant gene associated with improvement in ethanol productivity via ethanol fermentation and method for producing ethanol using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846531A (en) * 1990-03-21 1998-12-08 University Of Maryland Marine mela gene
US20190058193A1 (en) * 2015-10-09 2019-02-21 River Road Research, Inc. Method for producing lithium-ion, sodium-ion and potassium-ion batteries with increased safety
US20210180092A1 (en) * 2017-11-06 2021-06-17 Toyota Jidosha Kabushiki Kaisha Mutant gene associated with improvement in ethanol productivity via ethanol fermentation and method for producing ethanol using the same

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ALMEIDA-PAES RODRIGO, ALMEIDA-SILVA FERNANDO, PINTO GABRIELA COSTA MAIA, ALMEIDA MARCOS DE ABREU, MUNIZ MAURO DE MEDEIROS, PIZZINI: "L-tyrosine induces the production of a pyomelanin-like pigment by the parasitic yeast-form of Histoplasma capsulatum", MEDICAL MYCOLOGY, vol. 56, no. 4, 1 June 2018 (2018-06-01), Abingdon , pages 506 - 509, XP093108232, ISSN: 1369-3786, DOI: 10.1093/mmy/myx068 *
ARIAS-BARRAU ELSA, OLIVERA ELÍAS R., LUENGO JOSÉ M., FERNÁNDEZ CRISTINA, GALÁN BEATRIZ, GARCÍA JOSÉ L., DÍAZ EDUARDO, MIÑA: "The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida", JOURNAL OF BACTERIOLOGY, vol. 186, no. 15, 1 August 2004 (2004-08-01), US , pages 5062 - 5077, XP093108233, ISSN: 0021-9193, DOI: 10.1128/JB.186.15.5062-5077.2004 *
CARREIRA ALEXANDRA, FERREIRA LUÍSA M., LOUREIRO VIRGÍLIO: "Brown Pigments Produced by Yarrowia lipolytica Result from Extracellular Accumulation of Homogentisic Acid", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 8, 1 August 2001 (2001-08-01), US , pages 3463 - 3468, XP093108235, ISSN: 0099-2240, DOI: 10.1128/AEM.67.8.3463-3468.2001 *
GALEB HANAA A., LAMANTIA ANGELO, ROBSON ALEXANDER, KÖNIG KATJA, EICHHORN JONAS, BALDOCK SARA J., ASHTON MARK D., BAUM JOHN V., MOR: "The Polymerization of Homogentisic Acid In Vitro as a Model for Pyomelanin Formation", MACROMOLECULAR CHEMISTRY AND PHYSICS, vol. 223, no. 6, 1 March 2022 (2022-03-01), DE , pages 1 - 10, XP093108239, ISSN: 1022-1352, DOI: 10.1002/macp.202100489 *
MILLER KELLY K., SPRINGTHORPE SARAH K., IMBROGNO JENNIFER, WALKER DAVID J. F., GADIYAR SHRUTI, KEITZ BENJAMIN K., ELLINGTON ANDREW: "Biocompatible Materials Enabled by Biobased Production of Pyomelanin Isoforms Using an Engineered Yarrowia lipolytica", ADVANCED FUNCTIONAL MATERIALS, vol. 32, no. 7, 1 February 2022 (2022-02-01), DE , pages 1 - 11, XP093108243, ISSN: 1616-301X, DOI: 10.1002/adfm.202109366 *
TOKUHARA YASUNORI, SHUKUYA KENICHI, TANAKA MASAMI, MOURI MARIKO, OHKAWA RYUNOSUKE, FUJISHIRO MIDORI, TAKAHASHI TOMOO, OKUBO SHIGEO: "Detection of Novel Visible-Light Region Absorbance Peaks in the Urine after Alkalization in Patients with Alkaptonuria", PLOS ONE, vol. 9, no. 1, 23 January 2014 (2014-01-23), US , pages 1 - 7, XP093108230, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0086606 *

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