WO2023043397A1 - An identification-detection system and method - Google Patents

An identification-detection system and method Download PDF

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Publication number
WO2023043397A1
WO2023043397A1 PCT/TR2022/050232 TR2022050232W WO2023043397A1 WO 2023043397 A1 WO2023043397 A1 WO 2023043397A1 TR 2022050232 W TR2022050232 W TR 2022050232W WO 2023043397 A1 WO2023043397 A1 WO 2023043397A1
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Prior art keywords
biosensor
sample
enables
light
light source
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PCT/TR2022/050232
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French (fr)
Inventor
Şenay ÇİL
Hilda ÇİL
Original Assignee
Chi̇l Tibbi̇ Malzeme Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇
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Publication of WO2023043397A1 publication Critical patent/WO2023043397A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7796—Special mountings, packaging of indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/063—Illuminating optical parts
    • G01N2201/0634—Diffuse illumination

Definitions

  • the invention relates to a system and method for detection and identifying elements in a substance to be analyzed.
  • the invention particularly relates to an identification-detection system and method that enables the identification and analysis of specific substances in milk, toxic substances in treatment systems, specific substances in oil, and the detection of viral and bacterial infections in the medical sector, in the field of environment, agriculture, chemistry and food, for example in agriculture and food industry.
  • the channel system makes it difficult to transport devices by increasing their size.
  • the sample transmitted to the measurement area with the pump is transferred to the measurement area with the help of liquid (transferred at certain flow rates with water). Meanwhile, a certain amount of time is lost until the sample reaches the measurement area.
  • the measurement starts and a graph is revealed when the sample reaches the measurement area, and this graph is identified and detected with interpretation.
  • the measurement method used by the products in the state of art cannot be used with the desired precision.
  • Object of the invention is to solve the abovementioned disadvantages by being inspired from the current conditions.
  • the main object of the invention is to develop a system and method depending on the color change revealed on the substance to be identified and detected.
  • Another object of the invention is to create a system and method that enables more detailed and stable results to be obtained compared to the method based on the wavelength change used in substance identification and analysis in the state of art.
  • Another object of the invention is to design a system and method that minimizes the margin of error by using sensors with a high number of pixels.
  • the invention is a system that enables component identification and analysis of a sample taken from a substance to be analyzed in order to fulfill the abovedescribed objects. Accordingly, the system characterized by comprising:
  • biosensor which comprises and enables the interaction of a specific analyte with immobilized (fixed) biomolecules/chemicals on the gold-nanoparticle- coated substrate, and wherein the biosensor having: o the substrate on which the molecules are immobilized (fixed) and which enables processing on the molecules, o gold-nano particles that are specially synthesized with gold(lll) chloride trihydrate, resonated by absorbing light and coated on the said substrate, o analyte that forms bonds when reacting with the sample, and changes ambient light thanks to the bond formed, lens group that allows light from the said light source to be directed/concentrated on the biosensor,
  • the invention also comprises a method that enables component identification and analysis of a sample taken from a substance to be analyzed. Accordingly, the method characterized by comprising:
  • Figure 1 is a view of the system according to the invention.
  • the invention is a system that enables component identification and analysis of a sample taken from a substance to be analyzed.
  • the system comprises: a light source (1 ) which generates the light which enables the resonance of the nanoparticles, a substrate on which the molecules are immobilized (fixed) and which enables the processing of the molecules, gold-nano particles which are specially synthesized with gold(lll) chloride trihydrate, which are resonated by absorbing the light and coated on the said substrate, biosensor which forms bonds when they react with the sample, which contain analyte which changes the ambient light thanks to the bond formed and which enable the interaction of a specific analyte with immobilized (fixed) biomolecules/chemicals on the gold-nanoparticle-coated substrate, a lens group that allows the light from the said light source (1 ) to be directed/concentrated on the biosensor, an image sensor (8) that allows the image of the color change
  • the said analyte is characterized as, for example, biological molecule, antibody/antigen, enzymes/ligands, nucleic acids/oligonucleotide cellular structures/cells or biomimetic materials in the preferred embodiment of the system.
  • the system comprises a diffuser (2) that homogeneously distributes random photons from the light source (1 ) in a preferred embodiment of the invention.
  • the system contains cross-linking agents, which are used to detect which disease the molecules on the biosensor carry, for example, in the diagnosis of disease, and which are coated on the surface by immobilization method.
  • Cross-linking agents also ensure that the analyte is firmly attached to the surface coated with gold nanoparticles. Immobilization is performed by using cross-linking agents on the surface coated with gold nanoparticles.
  • the EDC together with NHS, allows two-step binding of two proteins without affecting the carboxyls of the second protein. First, EDC activates the carboxyl groups and forms an amine reactive O-acylisourea intermediate, which spontaneously reacts with primary amines to form an amide bond and an isourea by-product. It is ensured that the analyte to be immobilized on the surface makes a covalent bond with the help of cross-linking agents.
  • the aforementioned lens group comprises: a collecting lens (3) that focuses the scattered light beams coming out of the diffuser (2) to a single point, an iris (4) that adjusts the angle of spread of the light beams coming out of the collecting lens (3), and a smoothing lens (5) that turns the light beams nanoparticles coming out of the iris (4) into parallel rays.
  • An empty biosensor is placed in the relevant area in the system.
  • the light produced by the light source (1 ) is passed over an empty biosensor without any sample on it. Random photons from the light source (1 ) are homogeneously distributed by the diffuser (2).
  • the scattered light beams coming out of the diffuser (2) are focused on a single point by the collecting lens (3), the angle of spread of the light beams is adjusted by the iris (4), and the light beams coming out of the iris (4) are turned into parallel rays by the smoothing lens (5).
  • the light source (1 ) mentioned in the preferred embodiment of the system is the white light source.
  • the white light is dropped onto the image sensor (3), which is preferably characterized as a CCD-CMOS camera. Measurement is then taken from the empty biosensor.
  • the image of the measurement taken from the biosensor is taken from the image sensor (8).
  • the light beam coming out of the biosensor emits light at a certain wavelength depending on the structure of the nanoparticles, so there is a need to eliminate unwanted wavelengths.
  • the system comprises a visible region filter (7) that eliminates unwanted wavelengths in a preferred embodiment of the invention.
  • the HSV formula on the matrices is read over these RGB values and seen in 3 different values by taking the RGB values taken from the image (matrix). These values can be read as H (Hue), S (saturation), and V (value).
  • H value is between 0 and 1.
  • the values 0 and 1 are the minimum (min) and maximum (max) values and represent the red color.
  • the values between min-max represent other colors in the visible spectrum. These values are taken as a reference after the HSV values of the empty biosensor are measured.
  • the sample is delivered to the system from the sample inlet, preferably by means of a pasteur pipette or syringe.
  • the sample is spread on the biosensor thanks to the optisensor (6).
  • the sample is placed homogeneously on the biosensor.
  • the substrate on the biosensor is preferably glass material in order to ensure the permeability of the light, and it is ensured that the sample placed on the biosensor is placed homogeneously between the two glasses by placing an additional empty glass on the material.
  • the image is taken again through the image sensor while the reaction is carried out on the substrate surface on the biosensor. The results of this image are compared with the results of the first image with the help of a computer and a delta result is found.
  • the desired result is determined by applying the necessary formulations (fitting process) and the result is observed from the screen on the system with this delta result.
  • the RGB values taken from each pixel of the image sensor (8) are converted to HSV format and the curve of the Hue value, which is the first step, is extracted from the multiplicity of the analytes in the fitting process.
  • This curve is adapted to a function in such a way that its standard deviation is at least linear or parabolic.
  • the quantitative rate of the disease is determined from the number of analytes connected as output to the Hue values obtained by the image sensor (8) and the computer.

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention particularly relates to an identification-detection system and method that enables the identification and analysis of specific substances in milk, toxic substances in treatment systems, specific substances in oil, and the detection of viral and bacterial infections in the medical sector, in the field of environment, agriculture, chemistry and food, for example in agriculture and food industry.

Description

AN IDENTIFICATION-DETECTION SYSTEM AND METHOD
Technical Field of the Invention
The invention relates to a system and method for detection and identifying elements in a substance to be analyzed.
The invention particularly relates to an identification-detection system and method that enables the identification and analysis of specific substances in milk, toxic substances in treatment systems, specific substances in oil, and the detection of viral and bacterial infections in the medical sector, in the field of environment, agriculture, chemistry and food, for example in agriculture and food industry.
State of the Art
Today, studies are carried out on substance analysis and the determination of the components in it.
There is a channel system in the devices that perform substance analysis in the state of art. The channel system makes it difficult to transport devices by increasing their size. After sampling in the channel system, the sample transmitted to the measurement area with the pump is transferred to the measurement area with the help of liquid (transferred at certain flow rates with water). Meanwhile, a certain amount of time is lost until the sample reaches the measurement area. The measurement starts and a graph is revealed when the sample reaches the measurement area, and this graph is identified and detected with interpretation. The measurement method used by the products in the state of art cannot be used with the desired precision.
Currently, the measurement methods applied in the art operate on a spectroscopy basis, and the measurement accuracy is low since the current methods have a signal processing-based working principle. The algorithm used by the existing products is used only to detect the wavelength change and measures the changes in the wavelength with precision around 0.2 nanometers. This causes the changes in small quantities to be overlooked; therefore, the change load in small quantities cannot be detected. As a result, due to the abovementioned disadvantages and the insufficiency of the current solutions regarding the subject matter, a development is required to be made in the relevant technical field.
Object of the Invention
Object of the invention is to solve the abovementioned disadvantages by being inspired from the current conditions.
The main object of the invention is to develop a system and method depending on the color change revealed on the substance to be identified and detected.
Another object of the invention is to create a system and method that enables more detailed and stable results to be obtained compared to the method based on the wavelength change used in substance identification and analysis in the state of art.
Another object of the invention is to design a system and method that minimizes the margin of error by using sensors with a high number of pixels.
The invention is a system that enables component identification and analysis of a sample taken from a substance to be analyzed in order to fulfill the abovedescribed objects. Accordingly, the system characterized by comprising:
• light source that generates light that enables resonance of nanoparticles,
• biosensor which comprises and enables the interaction of a specific analyte with immobilized (fixed) biomolecules/chemicals on the gold-nanoparticle- coated substrate, and wherein the biosensor having: o the substrate on which the molecules are immobilized (fixed) and which enables processing on the molecules, o gold-nano particles that are specially synthesized with gold(lll) chloride trihydrate, resonated by absorbing light and coated on the said substrate, o analyte that forms bonds when reacting with the sample, and changes ambient light thanks to the bond formed, lens group that allows light from the said light source to be directed/concentrated on the biosensor,
• image sensor that enables the image of the color change arising from the activation of biological molecules as a result of the reaction,
• optisensor that keeps the said biosensor stationary, transfers the sample to the biosensor, and allows the sample to be propagated on the biosensor,
• the sample inlet section providing the sample inlet.
The invention also comprises a method that enables component identification and analysis of a sample taken from a substance to be analyzed. Accordingly, the method characterized by comprising:
• passing the white light produced by a light source through an empty biosensor which enables the interaction of a specific analyte with immobilized (fixed) biomolecules/chemicals on the gold-nanoparticle-coated substrate by the lens group which enables the light to be directed, and which does not contain any samples,
• receiving the first image through the image sensor that can detect color changes through the biosensor,
• storing the received image for later use,
• transferring the sample taken by a sample inlet section to the biosensor by an optisensor transferring the sample to the said biosensor and placing the sample homogeneously on the biosensor,
• receiving the second image through the image sensor after the reaction on the biosensor surface,
• calculating and comparing the Hue values of the images taken at the first and second reading and determining the result by applying fitting process to the result of the delta,
• displaying the generated result on a screen.
The structural and characteristic features and all the advantages of the invention will be understood more clearly by means of the figures and the detailed description with reference to these figures given below and therefore, the evaluation should be made by taking these figures and the detailed description into consideration.
Figures for Understanding of the Invention
Figure 1 is a view of the system according to the invention.
Description of the Part References
1. Light source
2. Diffuser
3. Collecting lens
4. Iris
5. Smoothing lens
6. Optisensor
7. Visible region filter
8. Image sensor
Detailed Description of the Invention
The preferred embodiments of the inventive system and method is described only for clarifying the subject in this detailed description. The invention is a system that enables component identification and analysis of a sample taken from a substance to be analyzed. Accordingly, the system comprises: a light source (1 ) which generates the light which enables the resonance of the nanoparticles, a substrate on which the molecules are immobilized (fixed) and which enables the processing of the molecules, gold-nano particles which are specially synthesized with gold(lll) chloride trihydrate, which are resonated by absorbing the light and coated on the said substrate, biosensor which forms bonds when they react with the sample, which contain analyte which changes the ambient light thanks to the bond formed and which enable the interaction of a specific analyte with immobilized (fixed) biomolecules/chemicals on the gold-nanoparticle-coated substrate, a lens group that allows the light from the said light source (1 ) to be directed/concentrated on the biosensor, an image sensor (8) that allows the image of the color change arising from the activation of the biological molecules as a result of the reaction, an optisensor (6) that keeps the said biosensor constant, transfers the sample to the biosensor and allows the sample to be spread on the biosensor, and a sample entry section that allows the sample to be entered.
The said analyte is characterized as, for example, biological molecule, antibody/antigen, enzymes/ligands, nucleic acids/oligonucleotide cellular structures/cells or biomimetic materials in the preferred embodiment of the system.
The system comprises a diffuser (2) that homogeneously distributes random photons from the light source (1 ) in a preferred embodiment of the invention.
In the preferred application of the invention, the system contains cross-linking agents, which are used to detect which disease the molecules on the biosensor carry, for example, in the diagnosis of disease, and which are coated on the surface by immobilization method. Cross-linking agents also ensure that the analyte is firmly attached to the surface coated with gold nanoparticles. Immobilization is performed by using cross-linking agents on the surface coated with gold nanoparticles. The EDC, together with NHS, allows two-step binding of two proteins without affecting the carboxyls of the second protein. First, EDC activates the carboxyl groups and forms an amine reactive O-acylisourea intermediate, which spontaneously reacts with primary amines to form an amide bond and an isourea by-product. It is ensured that the analyte to be immobilized on the surface makes a covalent bond with the help of cross-linking agents.
The aforementioned lens group comprises: a collecting lens (3) that focuses the scattered light beams coming out of the diffuser (2) to a single point, an iris (4) that adjusts the angle of spread of the light beams coming out of the collecting lens (3), and a smoothing lens (5) that turns the light beams nanoparticles coming out of the iris (4) into parallel rays.
The operating principle of the invention is as follows:
An empty biosensor is placed in the relevant area in the system. The light produced by the light source (1 ) is passed over an empty biosensor without any sample on it. Random photons from the light source (1 ) are homogeneously distributed by the diffuser (2). The scattered light beams coming out of the diffuser (2) are focused on a single point by the collecting lens (3), the angle of spread of the light beams is adjusted by the iris (4), and the light beams coming out of the iris (4) are turned into parallel rays by the smoothing lens (5). The light source (1 ) mentioned in the preferred embodiment of the system is the white light source. The white light is dropped onto the image sensor (3), which is preferably characterized as a CCD-CMOS camera. Measurement is then taken from the empty biosensor. The image of the measurement taken from the biosensor is taken from the image sensor (8). The light beam coming out of the biosensor emits light at a certain wavelength depending on the structure of the nanoparticles, so there is a need to eliminate unwanted wavelengths. The system comprises a visible region filter (7) that eliminates unwanted wavelengths in a preferred embodiment of the invention.
The HSV formula on the matrices is read over these RGB values and seen in 3 different values by taking the RGB values taken from the image (matrix). These values can be read as H (Hue), S (saturation), and V (value). The H value is between 0 and 1. The values 0 and 1 are the minimum (min) and maximum (max) values and represent the red color. The values between min-max represent other colors in the visible spectrum. These values are taken as a reference after the HSV values of the empty biosensor are measured.
The sample is delivered to the system from the sample inlet, preferably by means of a pasteur pipette or syringe. The sample is spread on the biosensor thanks to the optisensor (6). The sample is placed homogeneously on the biosensor. The substrate on the biosensor is preferably glass material in order to ensure the permeability of the light, and it is ensured that the sample placed on the biosensor is placed homogeneously between the two glasses by placing an additional empty glass on the material. The image is taken again through the image sensor while the reaction is carried out on the substrate surface on the biosensor. The results of this image are compared with the results of the first image with the help of a computer and a delta result is found. The desired result is determined by applying the necessary formulations (fitting process) and the result is observed from the screen on the system with this delta result. The RGB values taken from each pixel of the image sensor (8) are converted to HSV format and the curve of the Hue value, which is the first step, is extracted from the multiplicity of the analytes in the fitting process. This curve is adapted to a function in such a way that its standard deviation is at least linear or parabolic. Subsequently, the quantitative rate of the disease is determined from the number of analytes connected as output to the Hue values obtained by the image sensor (8) and the computer.

Claims

1. A system that enables component identification and analysis of a sample taken from a substance to be analyzed, characterized by comprising:
• light source (1 ) that generates light that enables resonance of nanoparticles,
• biosensor which comprises and enables the interaction of a specific analyte with immobilized (fixed) biomolecules/chemicals on the gold-nanoparticle- coated substrate, and wherein the biosensor having: o the substrate on which the molecules are immobilized (fixed) and which enables processing on the molecules, o gold-nano particles that are specially synthesized with gold(lll) chloride trihydrate, resonated by absorbing light and coated on the said substrate, o analyte that forms bonds when reacting with the sample, and changes ambient light thanks to the bond formed,
• lens group that allows light from the said light source (1 ) to be directed/concentrated on the biosensor,
• image sensor (8) that enables the image of the color change arising from the activation of biological molecules as a result of the reaction,
• optisensor (6) that keeps the said biosensor stationary, transfers the sample to the biosensor, and allows the sample to be propagated on the biosensor,
• the sample inlet section providing the sample inlet.
2. The system according to claim 1 , characterized in that the said image sensor (3) is a CCD/CMOS camera.
3. The system according to claim 1 , characterized by comprising diffuser (2) that homogeneously distributes random photons from the light source (1 ).
4. The system according to claim 1 , characterized in that the said lens group comprises:
8 • collecting lens (3) that focuses the scattered light beams coming out of the diffuser (2) to a single point,
• iris (4) that adjusts the angle of spread of the light beams coming out of the collecting lens (3),
• smoothing lens (5) that turns the light beams nanoparticles coming out of the iris (4) into parallel rays.
5. The system according to claim 1 , characterized by comprising visible region filter (7) that eliminates unwanted wavelengths.
6. The system according to claim 1 , characterized in that the said substrate is made of glass material.
7. The system according to claim 1 , characterized in that the said light source (1 ) is white light source.
8. The system according to claim 1 , characterized by comprising crosslinking agents which are used to determine which disease the molecules on the biosensor carry a virus load and which are coated on the surface by immobilization method.
9. The system according to claim 1 , characterized by comprising computer performing the said fitting process.
10. The system according to claim 1 , characterized in that the said analyte is biological molecule, antibody/antigen, enzymes/ligands, nucleic acids/oligonucleotide cellular structures/cells or biomimetic material.
11. A method that enables component identification and analysis of a sample taken from a substance to be analyzed, characterized by comprising the following process steps:
• passing the white light produced by a light source (1 ) through an empty biosensor which enables the interaction of a specific analyte with immobilized (fixed) biomolecules/chemicals on the gold-nanoparticle-coated substrate by the lens group which enables the light to be directed, and which does not contain any samples,
9 • receiving the first image through the image sensor that can detect color changes through the biosensor,
• storing the received image for later use,
• transferring the sample taken by a sample inlet section to the biosensor by an optisensor (6) transferring the sample to the said biosensor and placing the sample homogeneously on the biosensor,
• receiving the second image through the image sensor (8) after the reaction on the biosensor surface,
• calculating and comparing the Hue values of the images taken at the first and second reading and determining the result by applying fitting process to the result of the delta,
• displaying the generated result on a screen.
12. The method according to claim 11 , characterized by comprising the step of detecting which disease the molecules on the biosensor carry a virus load by means of crosslinking agents coated on the surface by the immobilization method.
13. The method according to claim 11 , characterized in that the said image sensor (3) comprises the process step of reading the values of H (hue), S (saturation), V (value).
14.The method according to claim 11 , characterized by comprising the step of homogeneously distributing random photons from the light source (1 ) by means of a diffuser (2).
15. The method according to claim 11 , characterized by comprising the following process steps:
• focusing the scattered light beams on a single point by the collecting lens (3),
• adjusting the angle of spread of the light beams coming out of the collecting lens (3) by the iris (4),
10 • converting the light beams coming out of the iris (4) into parallel rays by the smoothing lens (5).
16. The method according to claim 11 , characterized by comprising the step of eliminating unwanted wavelengths by means of a visible region filter (7).
17.The method according to claim 11 , characterized in that the fitting process comprises the following steps:
• converting the RGB values taken from each pixel of the image sensor (8) to HSV format,
• obtaining the curve of the H (Hue) value to the bonding multiplicity of the analytes,
• adapting the said curve to a function in such a way that standard deviation is at minimum degree as linear or parabolic,
• determining the quantitative rate of the disease from the multiplicity of analytes connected as output to the obtained Hue values.
18. The method according to claim 11 , characterized by comprising the step of performing the said fitting by computer.
19. The method according to claim 11 , characterized by comprising the step of displaying the result of the said fitting by means of computer screen.
11
PCT/TR2022/050232 2021-09-14 2022-03-14 An identification-detection system and method WO2023043397A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2021/014408A TR2021014408A2 (en) 2021-09-14 2021-09-14 A DIAGNOSTIC-DETERMINATION SYSTEM AND METHOD
TR2021/014408 2021-09-14

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150038347A1 (en) * 2010-03-19 2015-02-05 The University of Wyoming,an institution of higher of the State of Wyoming Surface enhanced raman spectroscopy
CN109863391A (en) * 2016-10-05 2019-06-07 雅培实验室 Device and method for sample analysis
US10830703B1 (en) * 2019-03-14 2020-11-10 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150038347A1 (en) * 2010-03-19 2015-02-05 The University of Wyoming,an institution of higher of the State of Wyoming Surface enhanced raman spectroscopy
CN109863391A (en) * 2016-10-05 2019-06-07 雅培实验室 Device and method for sample analysis
US10830703B1 (en) * 2019-03-14 2020-11-10 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis

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