WO2023039373A3 - Crispr-cas effector polypeptides and method of use thereof - Google Patents

Crispr-cas effector polypeptides and method of use thereof Download PDF

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Publication number
WO2023039373A3
WO2023039373A3 PCT/US2022/075980 US2022075980W WO2023039373A3 WO 2023039373 A3 WO2023039373 A3 WO 2023039373A3 US 2022075980 W US2022075980 W US 2022075980W WO 2023039373 A3 WO2023039373 A3 WO 2023039373A3
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WO
WIPO (PCT)
Prior art keywords
present disclosure
crispr
cas effector
nucleic acid
disclosure provides
Prior art date
Application number
PCT/US2022/075980
Other languages
French (fr)
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WO2023039373A2 (en
Inventor
Basem AL-SHAYEB
Original Assignee
The Regents Of The University Of California
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Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Publication of WO2023039373A2 publication Critical patent/WO2023039373A2/en
Publication of WO2023039373A3 publication Critical patent/WO2023039373A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present disclosure provides CRISPR-Cas effector polypeptides, nucleic acids encoding same, and compositions comprising same. The present disclosure provides ribonucleoprotein complexes comprising a CRISPR-Cas effector polypeptide of the present disclosure and a guide nucleic acid. The present disclosure provides methods of modifying a target nucleic acid, using a CRISPR-Cas effector polypeptide of the present disclosure and a guide nucleic acid. The present disclosure provides methods of detecting a target nucleic acid, using a CRISPR-Cas effector polypeptide of the present disclosure of the present disclosure and a guide nucleic acid.
PCT/US2022/075980 2021-09-08 2022-09-06 Crispr-cas effector polypeptides and method of use thereof WO2023039373A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163241859P 2021-09-08 2021-09-08
US63/241,859 2021-09-08

Publications (2)

Publication Number Publication Date
WO2023039373A2 WO2023039373A2 (en) 2023-03-16
WO2023039373A3 true WO2023039373A3 (en) 2023-08-31

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Family Applications (1)

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PCT/US2022/075980 WO2023039373A2 (en) 2021-09-08 2022-09-06 Crispr-cas effector polypeptides and method of use thereof

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WO (1) WO2023039373A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190351074A1 (en) * 2017-02-07 2019-11-21 The Regents Of The University Of California Gene therapy for haploinsufficiency
WO2020181101A1 (en) * 2019-03-07 2020-09-10 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof
US20210093667A1 (en) * 2017-06-26 2021-04-01 The Broad Institute, Inc. Crispr/cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190351074A1 (en) * 2017-02-07 2019-11-21 The Regents Of The University Of California Gene therapy for haploinsufficiency
US20210093667A1 (en) * 2017-06-26 2021-04-01 The Broad Institute, Inc. Crispr/cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing
WO2020181101A1 (en) * 2019-03-07 2020-09-10 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AL-SHAYEB BASEM, SKOPINTSEV PETR, SOCZEK KATARZYNA M., STAHL ELIZABETH C., LI ZHENG, GROOVER EVAN, SMOCK DYLAN, EGGERS AMY R., PAU: "Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors", CELL, ELSEVIER, AMSTERDAM NL, vol. 185, no. 24, 1 November 2022 (2022-11-01), Amsterdam NL , pages 4574 - 4586.e16, XP093088536, ISSN: 0092-8674, DOI: 10.1016/j.cell.2022.10.020 *

Also Published As

Publication number Publication date
WO2023039373A2 (en) 2023-03-16

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