WO2023019759A1 - Micro-droplet generation device - Google Patents

Abstract

A micro-droplet generation device, comprising: a generation chip (1), wherein the generation chip (1) comprises first compartments (141) and second compartments (142), and the generation chip (1) can generate micro-droplets by using liquid samples from the first compartments (141) and the second compartments (142); a collection plate (3), wherein the collection plate (3) comprises collection holes (31); and a connection base (2), wherein the connection base (2) wraps the collection plate (3), the connection base (2) comprises a chip support (21), the generation chip (1) is placed on and limited by the chip support (21), and the collection plate (3) is detachably mounted onto the connection base (2).

Description

micro droplet generation device

This application claims the priority of the Chinese patent application CN202110961284.0 with the filing date of August 20, 2021. This application cites the full text of the above-mentioned Chinese patent application.

technical field

The invention relates to the field of biology, in particular to a micro droplet generating device.

Background technique

Micro-droplet technology is a micro-nano technology that uses the interaction between flow shear force and surface tension to divide continuous fluid into discrete droplets of nanoliter and below volume in micro-scale channels.

There are two main types of micro-droplets: gas-liquid phase droplets and liquid-liquid phase droplets. The liquid-liquid phase micro-droplets have the advantages of small size, no diffusion between droplet samples, avoiding cross-contamination between samples, stable reaction conditions, and rapid mixing under proper control.

Liquid-liquid phase droplets are divided into "oil-in-water", "water-in-oil", "oil-in-water-in-oil" and "water-in-oil-in-water" according to the difference between the continuous phase and the dispersed phase.

The micro-droplet generation system can generate "water-in-oil" or "oil-in-water" micro-droplets with diameters on the order of microns (ie, 10-1000 μm), providing high-sensitivity, high-resolution micro-droplets for many application scenarios in the fields of biology, chemistry and materials. Efficient and high-throughput research site.

The 96-well plate is a commonly used experimental consumable in the biological field, but the micro-droplet generation device in the prior art is not compatible with the 96-well plate, and cannot directly generate the micro-droplet sample into the 96-well plate. After the micro-droplet generation device generates micro-droplets, it often needs to transfer the micro-droplets to the 96-well plate by other means and tools. The process is complicated and the contamination of the micro-droplet samples is easy to occur during the process. Therefore, it is difficult to use it as an experimental system with high universality in the fields of biology, chemistry and materials.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the poor compatibility between the micro-droplet generating device and the 96-well plate in the prior art, and it is impossible to directly generate the micro-droplet sample into the 96-well plate. After the droplet generation device generates microdroplets, it often needs to transfer the microdroplets to the 96-well plate by other means and tools. .

The present invention solves the above technical problems through the following technical solutions:

A microdroplet generating device comprising:

generating a chip, the generating chip comprising a first compartment and a second compartment, the generating chip can generate liquid samples in the first compartment and the second compartment into micro-droplets;

A collection plate, the collection plate is a 96-well plate, and the collection plate includes collection holes;

A connection base, the connection base wraps the collection plate, the connection base includes a chip support, the generated chip is placed and limited on the chip support, the collection plate is detachably mounted on the connection base seat.

In this solution, the above structure is used to integrate the production chip and the 96-well plate as the collection plate through the connection base, so that the micro-droplets generated by the production chip can be directly collected by the 96-well plate as the collection plate. The collection plate is detachably installed on the connection base, and it is also convenient to disassemble directly after collecting the micro-droplets for subsequent operations. There is no need to transfer the micro-droplets through other means and tools. The process is simple and the micro-droplet samples are not easily polluted during the process.

Preferably, the generation chip includes a discharge tube through which the micro-droplets are discharged, and when the generation chip is placed and limited on the chip holder, the discharge tube extends into the collection hole.

In this solution, the above-mentioned structural form is adopted, and through the limitation of the chip holder, when the generation chip is placed on it, the sample discharge tube can directly extend into the collection hole of the collection plate, so that the generation chip is aligned with the collection plate. At the same time, extending the sampling tube into the collection hole can also reduce the height of the droplet drop, reduce the kinetic energy of the droplet drop, and avoid the impact of the droplet from destroying the structure of the droplet.

Preferably, the generating chip includes a plurality of chip modules, the chip module includes a plurality of chip units corresponding to the collection holes one by one, and the plurality of chip units are integrally formed to form a chip module.

In this solution, the above-mentioned structure is adopted, and the generated chip is composed of multiple identical chip modules, and each chip module contains multiple chip units, and each chip unit can generate micro-droplets independently. The number of chip units and the collection The number of collection holes of the plate corresponds one to one. Each individual chip module is integrally formed, which facilitates the batch processing of the chip modules and reduces the production and maintenance costs of the generated chips.

Preferably, the chip module is formed of thermoplastic or thermosetting material or glass.

In this solution, by adopting the above-mentioned structural form, materials such as thermoplastic materials, thermosetting materials, and glass are easy to process, and the cost is low, further reducing the cost of producing chips.

Preferably, the generating chip includes:

A sample application layer, the sample application layer is arranged on the top of the generation chip, and the first compartment and the second compartment are provided on the sample application layer;

A sample layout layer, the sample layout layer is arranged at the bottom of the generation chip, the sample layout tube is arranged on the sample layout layer, the top surface of the sample layout layer and the bottom surface of the sample application layer are attached to each other and merged seal;

The micro-channel layer, the top surface of the sampling layer and the bottom surface of the sample application layer are attached and sealed to form the micro-channel layer, and the micro-channel layer includes the first compartment and the row The first flow channel communicated with the sample tube, the second flow channel communicated with the second compartment and the first flow channel, the second flow channel is along the vertical direction of the first flow channel and the first flow channel Generate area intersections.

In this scheme, the above-mentioned structural form is adopted. The microchannel layer is formed by bonding or pasting the sample loading layer and the sample layout layer. the cost of microdroplet generation.

Preferably, the top surface of the sampling layer and/or the bottom surface of the loading layer are recessed to form a first groove and a second groove, and the first groove and the second groove are connected with the The bottom surface of the sampling layer and/or the top surface of the sampling layer are attached and sealed to each other to form the first flow channel and the second flow channel.

In this solution, the above-mentioned structural form is adopted, and the generation chip generates micro-droplets through the first flow channel and the second flow channel, and the first flow channel and the second flow channel are formed by the The grooves on the bottom surface are formed. After the layout layer and the sample loading layer are packaged by bonding or adhesive bonding, these grooves become flow channels for the flow and intersection of liquid samples, generating micro-droplets, making the chip highly integrated.

Preferably, the first flow channel sequentially includes along the flow direction of the liquid sample: a first inlet connected to the first compartment, a buffer area, a generation area, and an outlet connected to the sample discharge pipe;

The buffer area includes a first extended flow channel and a second extended flow channel extending along the side of the first flow channel, and the first extended flow channel and the second extended flow channel communicate through a bent flow channel, so The first extended channel communicates with the first inlet, and the second extended channel communicates with the generating area.

In this scheme, the above structure is adopted, the liquid sample enters the first flow channel from the first inlet through the first compartment, and meets the liquid sample in the second flow channel in the generation area to form micro-droplets, and then self-discharges from the outlet Tube out. The buffer area is an extended section that is bent to one side and then returns to the direction of the original flow channel. A buffer area that is extended to one side is set between the first inlet and the generation area, so that the liquid sample can pass through the flow channel after entering the flow channel. Stabilize in the extended buffer area to make the flow rate and uniformity relatively stable before entering the production area to produce micro-droplets, which can improve the generation effect of micro-droplets.

Preferably, the first extended flow channel and the second extended flow channel are parallel to each other.

In this solution, the above-mentioned structural form is adopted, the first extended flow channel and the second extended flow channel are parallel to each other, the distance between the two can be kept constant and the maximum distance can be maintained, and the sample layer and the sample layer can be bonded or arranged. Packaging by means of adhesive stickers is easier and the packaging effect is better.

Preferably, the sampling tube includes:

an inner cavity, the cavity wall of which gradually converges from the top of the cavity to the bottom of the cavity;

a liquid discharge port, the liquid discharge port is opened on one side of the bottom of the inner cavity;

A diversion surface, the diversion surface is arranged at the bottom of the inner cavity, one end of the diversion surface is against the wall of the cavity, and the other end of the diversion surface is inclined downward and extends to the liquid discharge port.

In this scheme, the above-mentioned structural form is adopted, and the sample discharge tube is connected to the outlet of the microchannel layer. The bottom of the cavity flows to the diversion surface, and then slowly flows from the diversion surface to the discharge port to be discharged, which can avoid the direct drop of micro-droplets and excessive impact that may damage the structure of micro-droplets.

Preferably, the sample discharge tube further includes a drainage part, the drainage part is arranged on the cavity wall on the side where the liquid discharge port is opened in the inner cavity, and the drainage part protrudes along the cavity wall. rises and extends toward the drain port, and the projection of the end of the drainage part in the vertical direction is located on the guide surface.

In this solution, the above-mentioned structure is adopted, and a raised drainage part is provided above the cavity wall on the side where the discharge port is opened in the sample discharge tube, and the micro-droplets on the side cavity wall can be guided to the On the diversion surface, the micro-droplets are prevented from flowing directly from the side cavity wall from the liquid discharge port, and the diversion effect of the sample discharge tube is improved.

The positive progress effect of the present invention is: through the micro-droplet generation device disclosed in the present invention, the generation chip and the 96-well plate as the collection plate are integrated through the connection base, so that the micro-droplets generated by the production chip can be directly used as Collection plate for 96-well plate collection. The collection plate is detachably installed on the connection base, and it is also convenient to disassemble directly after collecting the micro-droplets for subsequent operations. There is no need to transfer the micro-droplets through other means and tools. The process is simple and the micro-droplet samples are not easily polluted during the process.

Description of drawings

FIG. 1 is a schematic structural diagram of a micro droplet generating device according to Embodiment 1 of the present invention.

FIG. 2 is a schematic diagram of the exploded structure of the micro-droplet generating device according to Embodiment 1 of the present invention.

FIG. 3 is a schematic diagram of a partial structure of a connecting base according to Embodiment 1 of the present invention.

FIG. 4 is a schematic structural diagram of a chip module according to Embodiment 1 of the present invention.

FIG. 5 is a schematic top view of the chip module according to Embodiment 1 of the present invention.

FIG. 6 is a schematic diagram of the connection structure between the chip module and the connection base according to Embodiment 1 of the present invention.

FIG. 7 is a schematic structural view of the microchannel layer in Example 1 of the present invention.

FIG. 8 is a schematic structural view of the microchannel layer in Example 1 of the present invention.

FIG. 9 is a schematic structural view of the microchannel layer in Example 1 of the present invention.

Fig. 10 is a schematic structural view of the sample-arranging tube according to Embodiment 2 of the present invention.

Fig. 11 is a schematic diagram of the internal structure of the sample-discharging tube according to Embodiment 2 of the present invention.

Explanation of reference signs:

generate chip 1

Loading layer 11

layout layer 12

Sample tube 121

Exit 122

Lumen 123

Drain 124

diversion surface 125

Drain 126

Chip Module 13

chip unit 14

first compartment 141

First entrance 143

Second entrance 144

Second compartment 142

Microfluidic layer 15

First runner 16

First extended runner 161

Second extended runner 162

Bend runner 163

Second runner 17

spawn area 18

Connect base 2

Chip Holder 21

Bump 211

Notch 212

Buckle 22

collection plate 3

Collection hole 31

Card slot 32

Detailed ways

The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples.

Embodiment one

As shown in FIGS. 1-3 , the droplet generating device of this embodiment includes a generating chip 1 , a collecting plate 3 and a connection base 2 . The generation chip 1 includes a first compartment 141 and a second compartment 142 , and the generation chip 1 can generate liquid samples in the first compartment 141 and the second compartment 142 into micro-droplets. The collection plate 3 is a 96-well plate, and the collection plate 3 includes collection holes 31 . The connection base 2 wraps the collection plate 3 , the connection base 2 includes a chip holder 21 , the generated chip 1 is placed and limited on the chip holder 21 , and the collection plate 3 is detachably mounted on the connection base 2 .

In this embodiment, the first compartment 141 and the second compartment 142 of the generation chip 1 are used to inject the water phase liquid and the oil phase liquid respectively. The two compartments are respectively pressurized by a precision air control device, so that the liquid passes through the generation chip 1 to generate "water-in-oil" or "oil-in-water" micro-droplets, which are discharged into the collection hole 31 of the collection plate 3 .

The collection plate 3 of this embodiment is a 96-well plate, which is a commonly used experimental consumable in the field of biology. The plate has 8*12 collection holes 31 . In other embodiments, the collecting plate 3 can also be composed of several 8-connected tubes.

As shown in Figures 2 and 3, the connecting base 2 of this embodiment is a square frame, and the connecting base 2 can be sleeved on the collecting plate 3 and wrapped around the collecting plate 3. The buckle 22 is detachably fixed to the slot 32 on the collecting plate 3 . Make its connection with the collecting plate 3 more stable. The slots 32 are arranged on both sides of the collecting plate 3 , and the buckles 22 are arranged at corresponding positions on both sides of the connecting base 2 . The buckle 22 is arranged on one end of a connecting plate that can be bent to a certain extent, the other end of the connecting plate is fixed on the side of the connecting base 2, and the back side of the end of the connecting plate provided with the buckle 22 is also provided with a handle portion. Extend to the other end of the connecting plate. By squeezing the handle part, the connecting plate can be bent outwards, so that the buckle 22 is lifted so as to be inserted into or disengaged from the slot 32 of the collecting plate 3 .

As shown in Figures 2 and 3, the chip holder 21 is composed of a plurality of groups of corresponding bumps 211 arranged at intervals on the top of the two opposite sides of the connection base 2. When the chip is placed on the connection base 2, the edge of the chip Then it is clamped in the notch 212 between the two protrusions 211 , and aligned with the collecting plate 3 clamped on the connection base 2 .

In other embodiments, the connecting base 2 can also be connected with the collecting plate 3 in other ways without sheathing the collecting plate 3, such as making an upper frame structure and installing it on the top surface of the collecting plate 3, or making it into a base structure Put the collecting plate 3 directly into the connection base 2. And the chip holder 21 is not limited to the bump 211 and the notch 212 that are arranged on the edge, but also adopts a top surface that is provided with a mounting groove on the top of the connection base 2, or inside the connection base 2, the top of the collecting plate 3 It can be realized by adding a support structure on the surface, as long as the detachable installation of the collecting plate 3 and the fixing and limiting of the generation chip 1 can be realized.

The generation chip 1 is integrated with the 96-well plate as the collection plate 3 through the connection base 2, so that the micro-droplets generated by the production chip can be directly collected by the 96-well plate as the collection plate 3. The collection plate 3 is detachably installed on the connection base 2, and it is also convenient to disassemble directly after collecting the micro-droplets for subsequent operations, without using other means and tools to transfer the micro-droplets, the process is simple and the micro-droplet samples are not easily polluted during the process .

As shown in FIG. 2 , the generation chip 1 includes a discharge tube 121 through which micro-droplets are discharged. When the generation chip 1 is placed and limited to the chip holder 21 , the discharge tube 121 extends into the collection hole 31 .

In this embodiment, the sampling tube 121 is arranged on the bottom surface of the generation chip 1 and extends downward. When the generation chip 1 is stuck in the notch 212, the sampling tube 121 of the generation chip 1 faces the collection port and extends into the into the collection hole 31.

Through the limitation of the chip holder 21 , when the generation chip 1 is placed on it, its sample tube 121 can directly extend into the collection hole 31 of the collection plate 3 , so that the generation chip 1 is aligned with the collection plate 3 . Simultaneously, extending the sampling tube 121 into the collecting hole 31 can also reduce the height of the droplet, reduce the kinetic energy of the droplet, and avoid the impact of the droplet from destroying the structure of the droplet.

In other embodiments, the sample discharge port can only be aligned with the collection hole 31 without extending into the collection hole 31, but this structure can only ensure that the micro-droplets drip into the collection hole 31 but cannot reduce the dripping of the micro-droplets. kinetic energy.

As shown in FIGS. 1-5 , the generation chip 1 includes several chip modules. The chip module includes several chip units 14 corresponding to the collection holes 31 one by one. The chip units 14 are integrally formed to form a chip module.

In this embodiment, each chip unit 14 can independently generate micro-droplets, including a set of first compartments 141, second compartments 142 and sample tubes 121, and a single chip module has 8 chips arranged horizontally Unit 14. The generation chip 1 of this embodiment is composed of 12 chip modules, and has 8*12 chip units 14 in total, corresponding to the 96-well plate as the collection plate 3 .

In other embodiments, the arrangement direction and quantity of the chip units 14 of the chip module 13 are not limited thereto, and can be adjusted according to different usage scenarios and different requirements, as long as it can correspond to the collection hole 31 of the collection plate 3. Can.

The generation chip 1 is composed of a plurality of identical chip modules, and each chip module includes a plurality of chip units 14, each chip unit 14 can generate micro-droplets independently, and the number of chip units 14 is the same as that of the collection hole 31 of the collection plate 3. corresponding to each other. Each individual chip module is integrally formed, which facilitates the batch processing of the chip modules and reduces the production and maintenance costs of generating the chip 1 .

In this embodiment, the chip module is formed of thermoplastic material, thermosetting material, or glass. Such materials are easy to process and low in cost, further reducing the cost of producing the chip 1 .

In other embodiments, other common thermoforming materials can also be used to make the product.

As shown in FIGS. 2-8 , the generation chip 1 includes a sample application layer 11 , a sample discharge layer 12 and a microchannel layer 15 . The sample application layer 11 is arranged on the top of the generation chip 1, and the first compartment 141 and the second compartment 142 are arranged on the sample application layer 11; On the sample layer 12, the top surface of the sample layout layer 12 and the bottom surface of the sample application layer 11 are mutually bonded and sealed; The layer 15 includes a first flow channel 16 communicating with the first compartment 141 and the sample discharge pipe 121, a second flow channel 17 communicating with the second compartment 142 and the first flow channel 16, and the second flow channel 17 is along the first flow channel 16. The vertical direction of and the first channel 16 intersect in the generation area 18 .

In this embodiment, the first compartment 141 communicates with the first flow channel 16 through the first inlet 143, the second compartment 142 communicates with the second flow channel 17 through the second inlet 144, and the second flow channel 17 is in the generation area 18 communicates with the first flow channel 16 from both sides. The liquid in the two compartments enters the two flow channels and flows toward the outlet 122 under the action of air pressure. When the liquid in the first compartment 141 flows through the generating region 18 in the first flow channel 16 , corresponding micro-droplets are generated under the flow shear force of the liquid in the second flow channel 17 .

In this embodiment, the top surface of the sample layout layer 12 and the bottom surface of the sample application layer 11 are attached to each other and packaged by bonding or other methods. In other embodiments, other common packaging methods such as adhesive stickers can also be used.

When the oil phase liquid is in the first compartment 141 and the water phase liquid is in the second compartment 142, "oil-in-water" micro-droplets can be generated;

When the liquid in the first compartment 141 is in the water phase and the liquid in the second compartment 142 is in the oil phase, "water-in-oil" micro-droplets can be generated.

The microchannel layer 15 in this embodiment is formed by bonding or pasting the sample application layer 11 and the sample discharge layer 12 . In other embodiments, an independent microchannel layer 15 may also be provided and communicated with the first inlet 143 , the second inlet 144 and the outlet 122 of the sampling layer 11 and the sampling layer 12 .

The microchannel layer 15 is formed by bonding or gluing the sample loading layer 11 and the sample layout layer 12. The three-layer structure of the chip is tightly stacked and fitted, highly integrated, small in area and volume, and significantly reduces the cost of microdroplet generation .

As shown in Figure 7, the bottom surface of the top surface of the sampling layer 12 and/or the sample application layer 11 is depressed to form the first groove and the second groove, and the first groove and the second groove pass through the contact with the sample application layer 11. The bottom surface and/or the top surface of the layout layer 12 form the first flow channel 16 and the second flow channel 17 by bonding or pasting.

In this embodiment, both the first groove and the second groove are formed on the bottom surface of the sample application layer 11 . The nesting layer 12 seals the grooves to form a flow channel by means of bonding or pasting.

In other embodiments, the groove can also be formed on the top surface of the layout layer 12 , or be formed on both surfaces respectively.

The generation chip 1 generates micro-droplets through the first flow channel 16 and the second flow channel 17, and the first flow channel 16 and the second flow channel 17 are formed by the concave holes on the top surface of the layout layer 12 and/or the bottom surface of the sample application layer 11. Grooves are formed, and after the sampling layer 12 and the sample loading layer 11 are packaged by bonding or adhesive bonding, these grooves become flow channels for liquid samples to flow and meet, and micro-droplets are generated to make the chip highly integrated.

As shown in FIG. 8 , the first flow channel 16 sequentially includes along the flow direction of the liquid sample: a first inlet 143 connected to the first compartment 141 , a buffer area, a generation area 18 and an outlet 122 connected to the sample discharge pipe 121 . The buffer area includes a first extended flow channel 161 and a second extended flow channel 162 extending along the side direction of the first flow channel 16, the first extended flow channel 161 and the second extended flow channel 162 communicate through a bent flow channel 163, the first extended flow channel The flow channel 161 communicates with the first inlet 143 , and the second extended flow channel 162 communicates with the generating area 18 .

In this embodiment, the second flow channel 17 extends from the position of the second outlet 122 from both sides respectively, and meets the first flow channel 16 from both sides of the vertical direction of the first flow channel 16 at the position of the generation area 18, and then Then lead to outlet 122 . The first channel 16 first passes through a buffer zone extended by bending from the position of the first inlet 143 , and then extends to the generating area 18 where it meets the second channel 17 , and then leads to the position of the outlet 122 .

As shown in FIG. 8 , the buffer area of this embodiment is composed of a first extended flow channel 161 , a curved flow channel 163 and a second extended flow channel 162 . The first extended flow channel 161 communicates with the first inlet 143 and extends laterally toward the first direction; the bent flow channel 163 communicates with the tail end of the first extended flow channel 161 and bends upwards in the longitudinal direction; the second extended flow channel 162 communicates with the bend The end of the flow channel 163 extends laterally toward a second direction, wherein the first direction is opposite to the second direction. Therefore, the first extended flow channel 161 , the curved flow channel 163 and the second extended flow channel 162 are connected in a U-shaped structure, and the entire buffer area is in a spiral structure.

In other embodiments, the buffer area can also be provided with more extended flow channels and curved flow channels 163 to form a multi-layer spiral structure to further extend the overall length of the buffer area and increase its flow stabilization effect. However, under the condition that the total length remains constant, using only one buffer zone with a bent structure can make the distance between the two extended flow channels the longest, and can reduce the gap between the sample loading layer 11 and the layout layer 12 by bonding or pasting, etc. The difficulty of encapsulation.

The liquid sample enters the first flow channel 16 from the first inlet 143 from the first compartment 141 , and meets the liquid sample in the second flow channel 17 in the generation area 18 to form micro-droplets, and then flows out from the sample discharge pipe 121 through the outlet 122 . The buffer area is an extended section that is bent to one side and then returns to the direction of the original flow channel. A buffer area extended to one side is set between the first inlet 143 and the generation area 18, so that the liquid sample can It can be stabilized in the extended buffer area to make the flow rate and uniformity relatively stable before entering the production area to produce micro-droplets, which can improve the generation effect of micro-droplets.

As shown in FIG. 8 , the first extension channel 161 and the second extension channel 162 are parallel to each other.

The first extended flow channel 161 and the second extended flow channel 162 are parallel to each other, so that the distance between the two can be kept constant and the maximum distance can be maintained, so that the sample loading layer 11 and the sample layout layer 12 can be packaged by bonding or adhesive bonding. Easier and better encapsulation.

As shown in FIG. 9 , the sampling tube 121 of this embodiment includes an inner cavity 123 and a liquid outlet 124 , and the wall of the inner cavity 123 gradually converges from the top of the inner cavity 123 to the bottom of the inner cavity 123 . The drain port 124 is opened at the bottom of the inner cavity 123 .

The inner cavity 123 is formed in the hollow of the sampling tube 121, and the top of the inner cavity 123 communicates with the outlet 122 of the microchannel layer 15, and the microdroplets formed by the microchannel layer 15 flow down from the outlet 122 along the wall of the inner cavity 123 . And drip from the drain port 124 at the bottom of the inner chamber 123 to the collecting hole 31 of the collecting plate 3 .

Embodiment two

The structure of the micro-droplet generation device in this embodiment is roughly the same as that in Embodiment 1, and the same parts thereof will not be described again. The difference lies in the sample-discharging tube 121 in this embodiment.

As shown in FIGS. 10 and 11 , the sample discharge tube 121 includes an inner cavity 123 , a liquid discharge port 124 and a flow guide surface 125 . The cavity wall of the inner cavity 123 gradually converges from the top of the inner cavity 123 to the bottom of the inner cavity 123 . The drain port 124 is opened on one side of the bottom of the inner cavity 123 . The flow guide surface 125 is disposed at the bottom of the inner cavity 123 , one end of the flow guide surface 125 is against the wall of the cavity, and the other end of the flow guide surface 125 is inclined downward and extends to the liquid outlet 124 .

In this embodiment, the interior of the sample discharge tube 121 is hollow to form an inner cavity 123, and the top of the inner cavity 123 communicates with the outlet 122 of the micro-channel layer 15, and the micro-droplets formed by the micro-channel layer 15 flow from the outlet 122 along the direction of the inner cavity 123. The cavity wall flows downward. The liquid discharge port 124 is opened at the bottom of the side of the sample discharge pipe 121 , and the flow guide surface 125 is provided at the bottom of the inner cavity 123 and slopes downward toward the liquid discharge port 124 along the wall of the inner cavity 123 .

The micro-droplets can flow along the cavity wall of the inner cavity 123 to the bottom of the cavity to the guide surface 125, and then slowly flow from the guide surface 125 to the discharge port 124 to be discharged, which can avoid the possible damage to the micro-droplets due to excessive impact. Micro-droplet structure.

As shown in Figures 10 and 11, the sample discharge tube 121 also includes a drainage part 126. The drainage part 126 is arranged on the cavity wall on the side where the liquid discharge port 124 is opened in the inner cavity 123. The drainage part 126 protrudes along the cavity wall and drains toward The liquid port 124 extends, and the projection of the end of the drainage part 126 in the vertical direction is located on the flow guiding surface 125 .

In this embodiment, the inclination angle of the drainage part 126 is slightly larger than the inclination angle of the cavity wall, and its end is located on the flow guide surface 125 in the vertical direction, so as to ensure that the micro-droplets dripping from the drainage part 126 can fall on the flow guide surface 125, to prevent micro-droplets from flowing out of the side chamber wall directly from the discharge port 124, and improve the diversion effect of the sample discharge tube 121.

Although the specific implementation of the present invention has been described above, those skilled in the art should understand that this is only an example, and the protection scope of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principle and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims (11)

1. A micro droplet generating device is characterized in that it comprises:

   generating a chip, the generating chip comprising a first compartment and a second compartment, the generating chip can generate liquid samples in the first compartment and the second compartment into micro-droplets;

   a collection plate comprising collection holes;

   A connection base, the connection base wraps the collection plate, the connection base includes a chip support, the generated chip is placed and limited on the chip support, the collection plate is detachably mounted on the connection base seat.
2. The micro droplet generating device according to claim 1, wherein the collecting plate is a 96-well plate.
3. The micro-droplet generation device according to claim 1 or 2, wherein the generation chip comprises a sample discharge tube, and the micro-droplets are discharged through the sample discharge tube, when the generation chip is placed and limited to When the chip is supported, the sample discharge tube extends into the collection hole.
4. The device for generating microdroplets according to at least one of claims 1-3, wherein the generating chip comprises a plurality of chip modules, and the chip module includes a plurality of chip units one-to-one corresponding to the collection holes, Several chip units are integrally molded to form a chip module.
5. The micro droplet generating device according to claim 4, wherein the chip module is formed of thermoplastic material or thermosetting material or glass material.
6. The microdroplet generating device according to at least one of claims 3-5, wherein the generating chip comprises:

   A sample application layer, the sample application layer is arranged on the top of the generation chip, and the first compartment and the second compartment are provided on the sample application layer;

   A sample layout layer, the sample layout layer is arranged at the bottom of the generation chip, the sample layout tube is arranged on the sample layout layer, the top surface of the sample layout layer and the bottom surface of the sample application layer are attached to each other and merged seal;

   The micro-channel layer, the top surface of the sampling layer and the bottom surface of the sample-loading layer are attached and sealed to form the micro-channel layer, and the micro-channel layer includes the first compartment and the row The first flow channel communicated with the sample tube, the second flow channel communicated with the second compartment and the first flow channel, the second flow channel is along the vertical direction of the first flow channel and the first flow channel Generate area intersections.
7. The micro-droplet generating device according to claim 6, wherein the top surface of the sample discharge layer and/or the bottom surface of the sample application layer are depressed to form a first groove and a second groove, and the first groove The first flow path and the second flow path are formed by a groove and the second groove being attached to and sealed with the bottom surface of the sample application layer and/or the top surface of the sample discharge layer.
8. The micro-droplet generating device according to claim 6 or 7, wherein the first flow channel sequentially comprises along the flow direction of the liquid sample: a first inlet communicating with the first compartment, a buffer area, a generating area, and a communication area. The outlet of the sample tube;

   The buffer area includes a first extended flow channel and a second extended flow channel extending along the side of the first flow channel, and the first extended flow channel and the second extended flow channel communicate through a bent flow channel, so The first extended channel communicates with the first inlet, and the second extended channel communicates with the generating area.
9. The micro droplet generating device according to claim 8, wherein the first extended channel and the second extended channel are parallel to each other.
10. The microdroplet generating device according to at least one of claims 3-9, wherein the sample discharge tube comprises:

    an inner cavity, the cavity wall of which gradually converges from the top of the cavity to the bottom of the cavity;

    a liquid discharge port, the liquid discharge port is opened on one side of the bottom of the inner cavity;

    A diversion surface, the diversion surface is arranged at the bottom of the inner cavity, the diversion surface is inclined downward from the position of the cavity wall and extends to the liquid discharge port.
11. The micro-droplet generating device according to claim 10, wherein the sample discharge tube further comprises a drainage part, and the drainage part is arranged in the cavity on the side where the liquid discharge port is opened in the inner cavity. On the wall, the drainage part protrudes along the chamber wall and extends toward the liquid discharge port, and the projection of the end of the drainage part in the vertical direction is located on the flow guide surface.

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