WO2023018878A3 - Sub-3 å cryo-em structure of rna enabled by engineered homomeric self-assembly - Google Patents

Sub-3 å cryo-em structure of rna enabled by engineered homomeric self-assembly Download PDF

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Publication number
WO2023018878A3
WO2023018878A3 PCT/US2022/040074 US2022040074W WO2023018878A3 WO 2023018878 A3 WO2023018878 A3 WO 2023018878A3 US 2022040074 W US2022040074 W US 2022040074W WO 2023018878 A3 WO2023018878 A3 WO 2023018878A3
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Prior art keywords
rna
cryo
homomeric
engineered
enabled
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PCT/US2022/040074
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French (fr)
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WO2023018878A2 (en
Inventor
Di LIU
Peng Yin
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President And Fellows Of Harvard College
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Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2023018878A2 publication Critical patent/WO2023018878A2/en
Publication of WO2023018878A3 publication Critical patent/WO2023018878A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The disclosure relates generally to methods and compositions for characterizing an RNA molecule, comprising modifying an RNA molecule by replacing or modifying a first peripheral domain with a first kissing loop and a second peripheral domain with a second kissing loop in the RNA molecule, and determination of RNA structures using single-particle cryogenic electron microscopy (cryo-EM).
PCT/US2022/040074 2021-08-11 2022-08-11 Sub-3 å cryo-em structure of rna enabled by engineered homomeric self-assembly WO2023018878A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163232119P 2021-08-11 2021-08-11
US63/232,119 2021-08-11

Publications (2)

Publication Number Publication Date
WO2023018878A2 WO2023018878A2 (en) 2023-02-16
WO2023018878A3 true WO2023018878A3 (en) 2023-06-01

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ID=85201064

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/040074 WO2023018878A2 (en) 2021-08-11 2022-08-11 Sub-3 å cryo-em structure of rna enabled by engineered homomeric self-assembly

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WO (1) WO2023018878A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120263648A1 (en) * 2009-06-16 2012-10-18 Government Of The United States Of America, As Represented By The Rna nanoparticles and nanotubes
US20210069345A1 (en) * 2018-05-08 2021-03-11 The United States Of America,As Represented By The Secretary,Department Of Health And Human Services Hexameric tetrahedral rna nanostructures

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120263648A1 (en) * 2009-06-16 2012-10-18 Government Of The United States Of America, As Represented By The Rna nanoparticles and nanotubes
US9732337B2 (en) * 2009-06-16 2017-08-15 The United Stated of America, as represented by the Secretary, Department of Health & Human Services RNA nanoparticles and nanotubes
US20210069345A1 (en) * 2018-05-08 2021-03-11 The United States Of America,As Represented By The Secretary,Department Of Health And Human Services Hexameric tetrahedral rna nanostructures

Also Published As

Publication number Publication date
WO2023018878A2 (en) 2023-02-16

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