WO2023015559A1 - Macrocycle compounds as inhibitors of ras - Google Patents

Macrocycle compounds as inhibitors of ras Download PDF

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Publication number
WO2023015559A1
WO2023015559A1 PCT/CN2021/112499 CN2021112499W WO2023015559A1 WO 2023015559 A1 WO2023015559 A1 WO 2023015559A1 CN 2021112499 W CN2021112499 W CN 2021112499W WO 2023015559 A1 WO2023015559 A1 WO 2023015559A1
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Prior art keywords
optionally substituted
membered
alkyl
combine
cycloalkyl
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PCT/CN2021/112499
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French (fr)
Inventor
Jian Zhang
Yong Li
Shaomin ZHANG
Caiming XI
Heyan SHAO
Kang HE
Long Wang
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Nutshell Biotech (Shanghai) Co., Ltd.
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Priority to PCT/CN2021/112499 priority Critical patent/WO2023015559A1/en
Publication of WO2023015559A1 publication Critical patent/WO2023015559A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains three hetero rings
    • C07D498/18—Bridged systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D515/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
    • C07D515/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains three hetero rings
    • C07D515/18—Bridged systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D515/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
    • C07D515/22—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains four or more hetero rings

Definitions

  • the present disclosure relates to macrocycle compounds, and pharmaceutical compositions including the same, that are inhibitors of one or more RAS proteins and are useful in the treatment of RAS-associated diseases such as cancer.
  • RAS proteins (K-Ras, H-Ras, N-Ras etc. ) play essential roles in various cancers. More importantly, various mutations in RAS proteins account for approximately 30%of all cancers studied. Among all RAS proteins, KRAS is the most frequently mutated RAS protein, and therefore is the most sought after target for cancer therapy. Fortunately, there is a small molecule drug inhibiting KRAS approved for clinical use after decade long extensive research. Considering that there are so many types of cancers related to KRAS proteins, it remains a clinical need for more small molecule drugs inhibiting KRAS proteins.
  • the inventors believe that modulating the activity of a protein can be achieved via a direct binding with a synthetic ligand to this protein or by interacting with another intracellular protein which forms complexes with this protein. In the later cases, a high affinity three component complex will be formed. The two intracellular proteins involved do not interact under normal conditions, but the addition of a synthetic ligand induces the formation of the three components conjugate which results in affecting the biological activity of the targeted protein. More specifically, the disclosed macrocyclic small molecules here are capable of perturbating biological processes through binding to a member of the cyclophilin A family (presenter protein) and a mutated RAS protein (target protein) .
  • A is -N (H or CH 3 ) C (O) - (CH 2 ) -where the amino nitrogen is bound to the carbon atom of -C (R 1 R 2 ) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
  • B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • D is optionally substituted C 1 -C 4 alkylene, optionally substituted C 1 -C 4 alkenylene, optionally substituted C 1 -C 4 heteroalkylene, -C (O) O-CH (R 11 ) -where CH (R 11 ) -is bound to -C (R 5 R 6 ) -, -C (O) NH-CH (R 11 ) -where CH (R 11 ) -is bound to -C (R 5 R 6 ) -, optionally substituted C 1 -C4 heteroalkylene, or 3 to 8-membered heteroarylene;
  • L is absent or a linker
  • W is H, D, cyano, S (O) 2 R’ , an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1 -C 4 alkoxy, optionally substituted C 1 -C 4 hydroxyalkyl, optionally substituted C 1 -C 4 aminoalkyl, optionally substituted C 1 -C 4 haloalkyl, optionally substituted C 1 -C 4 alkyl, optionally substituted C 1 -C 4 guanidinoalkyl, C 0 -C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
  • X 1 is NR, O, S (O) n or CR 13 , n is 0, 1 or 2;
  • X 2 is O, NH or CR 14 ;
  • X 3 is O, NH or CR 14a ;
  • X 4 is N or CH
  • R is H, D, cyano, optionally substituted C 1 -C 4 alkyl, optionally substituted C 2 -C 4 alkenyl, optionally substituted C 2 -C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’) 2 , S (O) R’, or S (O) 2 N (R’) 2 ; R’ is, independently, H, D or optionally substituted C 1 -C 4 alkyl;
  • R 1 is H, D, halo, hydroxy, C 1 -C 4 alkoxy, or C 1 -C 4 alkyl;
  • R 2 is H, D, halo, hydroxy, C 1 -C 4 alkoxy, or C 1 -C 4 alkyl;
  • R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1 -C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
  • R 5 is hydrogen, halogen, or optionally substituted C 1 -C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
  • R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1 -C 3 alkoxy, optionally substituted C 1 -C 3 alkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
  • R 5 ’ is hydrogen, halogen, or optionally substituted C 1 -C 3 alkyl
  • R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1 -C 3 alkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5 ’ and R 6 ’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
  • R 7 is H, D, C 1 -C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1 -C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
  • R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1 -C 3 alkyl, optionally substituted C 1 -C 3 alkoxyl, or
  • R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
  • R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • R 10 is H, D, or C 1 -C 3 alkyl, cyclopropyl, cyclobutyl;
  • R 11 is hydrogen or methyl
  • R 12 is hydrogen, F, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
  • R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl
  • R 12 ’ is hydrogen or optionally substituted C 1 -C 6 alkyl
  • R 13 is H, D, cyano, optionally substituted C 1 -C 4 alkyl, optionally substituted C 1 -C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
  • R 14 is H, D, cyano, optionally substituted C 1 -C 3 alkyl, optionally substituted alkoxy;
  • R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • R 14a is H, D, cyano, optionally substituted C 1 -C 3 alkyl, optionally substituted alkoxy.
  • compositions and methods which are described herein in the context of separate aspects, may also be provided in combination in a single aspect.
  • the expression “about 0.01%to about 1%” means any values between 0.01%and 1%, for example 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%and 1%.
  • Other similar expressions like “40%-50%to about 50%-70%” should also be understood in a similar manner.
  • mixture is intended to mean a mixture containing more than one species of compounds, wherein one or more species of compounds can be target compound (s) .
  • target compound means a compound to be separated or purified. When defining a separation process, the species of the target compound (s) are determined before the separation operations. It is to be understood that the product which contains the target compound (s) could be in any desired form, for example a product containing a pure isomer compound or a mixture containing a plurality of predefined species of the target compounds.
  • the substituents of an “optionally substituted” group may include, without limitation, one or more substituents independently selected from the following groups or a particular designated set of groups, alone or in combination, as defined herein: alkyl, alkenyl, alkynyl, alkanoyl, heteroalkyl, heterocycloalkyl, haloalkyl, haloalkenyl, haloalkynyl, perhaloalkyl, perhaloalkoxy, cycloalkyl, phenyl, aryl, aryloxy, alkoxy, haloalkoxy, oxo, acyloxy, carbonyl, carboxyl, alkylcarbonyl, carboxyester, carboxamido, cyano, hydrogen, halogen, hydroxy, amino, alkylamino, arylamino, amido, nitro, thiol, alkylthio, haloalkylthio, perhaloalkylthio,
  • Two substituents may be joined together to form a fused five-, six-, or seven-membered carbocyclic or heterocyclic ring comprising zero to three heteroatoms, for example forming methylenedioxy or ethylenedioxy.
  • An optionally substituted group may be unsubstituted (e.g., -CH 2 CH 3 ) , fully substituted (e.g., -CF 2 CF 3 ) , mono-substituted (e.g., -CH 2 CH 2 F) or substituted at a level anywhere in-between fully substituted and mono-substituted (e.g., -CH 2 CF 3 ) .
  • the chemical bond in the compound of the disclosure can be depicted herein with a solid line [——] , a wavy line asolid wedge or a dashed wedge It is intended that a bond to an asymmetric atom depicted with a solid line indicates that all possible stereoisomers at the atom (e.g., specific enantiomers, racemic mixtures and the like) are contemplated. It is intended that a bond to an asymmetric atom depicted with a wavy line indicates that the bond is either a solid wedge bond or a dashed wedge bond. It is intended that a bond to an asymmetric atom depicted with a solid or dashed wedge indicates the existence of the stereoisomer that is shown.
  • a solid or dashed wedge is used to define relative stereochemistry rather than absolute stereochemistry.
  • the compound of the disclosure can be present in the form of stereoisomers (including cis-and trans-isomers, optical isomers (e.g., R and S enantiomers) , diastereomers, geometric isomers, rotamers, conformers, atropisomers, and mixtures thereof) .
  • the compound of the disclosure can exhibit one or more types of the above isomerism and can be consisted of a mixture thereof (e.g., a racemic mixture and/or a diastereomeric pair) .
  • stable refers to a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and preferably capable of formulation into an efficacious therapeutic agent.
  • alkyl when used alone or as part of a substituent group, refers to a straight-or branched-chain hydrocarbon group having from 1 to 6 carbons atoms ( “C 1-6 ” ) , in the group.
  • alkyl groups include methyl (Me, C 1 alkyl) , ethyl (Et, C 2 alkyl) , n-propyl (C 3 alkyl) , isopropyl (C 3 alkyl) , butyl (C 4 alkyl) , isobutyl (C 4 alkyl) , sec-butyl (C 4 alkyl) , tert-butyl (C 4 alkyl) , pentyl (C 5 alkyl) , isopentyl (C 5 alkyl) , tert-pentyl (C 5 alkyl) , hexyl (C 6 alkyl) , isohexyl (C 6 alkyl)
  • haloalkyl when used alone or as part of a substituent group, refers to a straight or branched-chain hydrocarbon group having from 1 to 6 carbons atoms ( “C 1-6 ” ) in the group, wherein one or more of the hydrogen atoms in the group have been replaced by a halogen atom.
  • haloalkyl groups include trifluoromethyl (-CF 3 , C 1 haloalkyl) , trifluoroethyl (-CH 2 CF 3 , C 2 haloalkyl) , and the like.
  • alkylene when used alone or as part of a substituent group, refers to an alkyl diradical, i.e., a straight-or branched-chain hydrocarbon group having from 1 to 6 carbons atoms ( “C 1-6 ” ) , in the group, wherein the group is directly attached to two other variable groups.
  • cycloalkyl refers to non-aromatic cyclic hydrocarbons including cyclized alkyl and/or alkenyl groups. Cycloalkyl groups thus also encompass cycloalkenyl groups. Cycloalkyl groups can include mono-or polycyclic (e.g., having 2 or more than 2 fused rings) groups, spirocycles, and bridged rings (e.g., a bridged bicycloalkyl group) . Ring-forming carbon atoms of a cycloalkyl group can be optionally substituted by oxo or sulfido (e.g., C (O) or C (S) ) .
  • oxo or sulfido e.g., C (O) or C (S)
  • cycloalkyl moieties that have one or more aromatic rings fused (i.e., having a bond in common with) to the cycloalkyl ring, for example, benzo or thienyl derivatives of cyclopentane, cyclohexane, and the like.
  • a cycloalkyl group containing a fused aromatic ring can be attached through any ring-forming atom including a ring-forming atom of the fused aromatic ring.
  • Cycloalkyl groups can have 3, 4, 5, 6, 7, 8, 9, or 10 ring-forming carbons (C 3-10 ) .
  • the cycloalkyl is a C 3-10 monocyclic or bicyclic cyclocalkyl.
  • the cycloalkyl is a C 3-10 monocyclic or bicyclic cycloalkyl which is optionally substituted by CH 2 F, CHF 2 , CF 3 , and CF 2 CF 3 . In some embodiments, the cycloalkyl is a C 3-7 monocyclic cycloalkyl. In some embodiments, the cycloalkyl is a C 4-10 spirocycle or bridged cycloalkyl.
  • Example cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclopentenyl, cyclohexenyl, cyclohexadienyl, cycloheptatrienyl, norbornyl, nopinyl, norcarnyl, cubane, adamantane, bicyclo [l. 1.1] pentyl, bicyclo [2. l. l] hexyl, bicyclo [2.2. l] heptanyl, bicyclo [3.1.1] heptanyl, bicyclo [2.2.2] octanyl, spiro [3 .
  • cycloalkyl is cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl.
  • cycloalkyl are cyclic-containing, non-aromatic hydrocarbon groups having from 3 to 1 2 carbon atoms ( “C 3-12 ” ) , preferably from 3 to 6 carbon atoms ( “C 3-6 ” )
  • Examples of cycloalkyl groups include, for example, cyclopropyl (C 3 ; 3-membered) , cyclobutyl (C 4 ; 4-membered) , cyclopropylmethyl (C 4 ) , cyclopentyl (C 5 ) , cyclohexyl (C 6 ) , 1 -methylcyclopropyl (C 4 ) , 2-methylcyclopentyl (C 6 ) , adamantanyl (C 10 ) , and
  • cycloalkylene when used alone or as part of a substituent group refers to a cycloalkyl diradical, i.e., a cyclic-containing, non-aromatic hydrocarbon group having from 3 to 14 carbon atoms ( “C 3-14 ” ; or 3-14 membered) , for example 3 to 12 carbon atoms ( “C 3-12 ” ) , preferably from preferably from 3 to 7 carbon atoms ( “C 3-7 ” , or 3-7 membered) or 3 to 6 carbon atoms ( “C 3-6 ” ) , wherein the group is directly attached to two other variable groups.
  • Cycloalkylene groups include spirocycloalkylene groups.
  • cycloalkenylene refers to a cycloalkenylene diradical.
  • heteroalkylene refers to a heteroalkylene diradical.
  • spirocycloalkyl when used alone or as part of a substituent group refers to a non-aromatic hydrocarbon group containing two cycloalkyl rings, and wherein the two cycloalyl rings share a single carbon atom in common.
  • spirocycloalkylene when used alone or as part of a substituent group refers to a spirocycloalkyl diradical, i.e., a non-aromatic hydrocarbon group containing two cycloalkyl rings, and wherein the two cycloalyl rings share a single carbon atom in common, and wherein the group is directly attached to two other variable groups.
  • heterocycloalkyl refers to monocyclic or polycyclic heterocycles having at least one non-aromatic ring (saturated or partially unsaturated ring) , wherein one or more of the ring-forming carbon atoms of the heterocycloalkyl is replaced by a heteroatom selected from the group consisting of N, O, S and B, and wherein the ring-forming carbon atoms and heteroatoms of the heterocycloalkyl group can be optionally substituted by one or more oxo or sulfido (e.g., C (O) , S (O) , C (S) , or S (O) 2 , etc. ) .
  • oxo or sulfido e.g., C (O) , S (O) , C (S) , or S (O) 2 , etc.
  • Heterocycloalkyl groups include monocyclic and polycyclic (e.g., having 2 or more than 2 fused rings) systems. Included in heterocycloalkyl are monocyclic and polycyclic 4-10, 4-7, and 3-14 membered heterocycloalkyl groups. Heterocycloalkyl groups can also include spirocycles and bridged rings (e.g., a 5-10 membered bridged biheterocycloalkyl ring having one or more of the ring-forming carbon atoms replaced by a heteroatom independently selected from the group consisting of N, O, S and B) . The heterocycloalkyl group can be attached through a ring-forming carbon atom or a ring-forming heteroatom. In some embodiments, the heterocycloalkyl group contains 0 to 3 double bonds. In some embodiments, the heterocycloalkyl group contains 0 to 2 double bonds.
  • heterocycloalkyl moieties that have one or more aromatic rings fused (i.e., having a bond in common with) to the non-aromatic heterocyclic ring, for example, benzo or thienyl derivatives of piperidine, morpholine, azepine, etc.
  • a heterocycloalkyl group containing a fused aromatic ring can be attached through any ring-forming atom including a ring-forming atom of the fused aromatic ring.
  • the heterocycloalkyl group contains 4 to 10 ring-forming atoms, 4 to 7 ring-forming atoms, or 3 to 14 ring-forming atoms.
  • the heterocycloalkyl group has 1 to 4 heteroatoms, 1 to 3 heteroatoms, 1 to 2 heteroatoms or 1 heteroatom.
  • the heterocycloalkyl is a monocyclic 4-6 membered heterocycloalkyl having 1 or 2 heteroatoms independently selected from the group consisting of N, O, S and B and having one or more oxidized ring members.
  • Example heterocycloalkyl groups include pyrrolidin-2-one, l, 3-isoxazolidin-2-one, pyranyl, tetrahydropyran, oxetanyl, azetidinyl, morpholino, thiomorpholino, piperazinyl, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, pyrrolidinyl, isoxazolidinyl, isothiazolidinyl, pyrazolidinyl, oxazolidinyl, thiazolidinyl, imidazolidinyl, azepanyl, benzazapene, 1, 2, 3, 4-tetrahydroisoquinoline, 2, 3-dihydrobenzofuryl, 1, 3-benzodioxole, benzo-1, 4-dioxane, azabicyclo [3.1.0] hexanyl, diazabicyclo [3.1.0] he
  • heterocycloalkyl refers to any 3 to 10 membered monocyclic or bicyclic, saturated ring structure containing at least one heteroatom selected from the group consisting of O, N and S.
  • the heterocycloalkyl group may be attached at any heteroatom or carbon atom of the ring such that the result is a stable structure.
  • heterocycloalkyl groups include, but are not limited to, azepanyl, aziridinyl, azetidinyl, pyrrolidinyl, dioxolanyl, imidazolidinyl, pyrazolidinyl, piperazinyl, piperidinyl, dioxanyl, morpholinyl, dithianyl, thiomorpholinyl, oxazepanyl, oxiranyl, oxetanyl, quinuclidinyl, tetrahydrofuranyl, tetrahydropyranyl, piperazinyl, and the like.
  • heterocycloalkylene when used alone or as part of a substituent group refers to a heterocycloalkyl diradical.
  • heterocycloalkylene any three to ten membered monocyclic or bicyclic, saturated ring structure containing at least one heteroatom selected from the group consisting of O, N and S, wherein the ring structure is directly attached to two other variable groups.
  • alkenyl when used alone or as part of a substituent group refers to a straight-or branched-chain group having from 2 to 6 carbon atoms ( “C 2-6 ” ) , preferably 2 to 4 carbons atoms ( “C 2-4 ” ) , in the group, wherein the group includes at least one carbon-carbon double bond.
  • alkenylene when used alone or as part of a substituent group refers to a alkenyl diradical, i.e., a straight-or branched-chain group having from 2 to 6 carbon atoms ( “C 2-6 ” ) , preferably 2 to 4 carbons atoms ( “C 2-4 ” ) , in the group, wherein the group includes at least one carbon-carbon double bond, and wherein the group is directly attached to two other variable groups.
  • alkynyl when used alone or as part of a substituent group refers to a straight-or branched-chain group having from 2 to 6 carbon atoms ( “C 2-6 ” ) , preferably 2 to 4 carbons atoms ( “C 2-4 ” ) , in the group, wherein the group includes at least one carbon-carbon triple bond.
  • alkynyl groups include ethynyl (-C ⁇ CH; C 2 alkynyl) , propragyl (-CH 2 -CH ⁇ CH; C 3 alkynyl) , and the like.
  • alkynylene when used alone or as part of a substituent group refers to an alkynyl diradical, i.e., a straight-or branched-chain group having from 2 to 12 carbon atoms ( “C 2-12 ” ) , preferably 2 to 4 carbons atoms ( “C 2-4 ” ) , in the group, wherein the group includes at least one carbon-carbon triple bond, and wherein the group is directly attached to two other variable groups.
  • aryl when used alone or as part of a substituent group refers to a monocyclic all carbon aromatic ring or a multicyclic all carbon ring system wherein the rings are aromatic.
  • aryl refers to a mono-or bicyclic-aromatic hydrocarbon ring structure having 6-10 carbon atoms in the ring, wherein one or more of the carbon atoms in the ring is optionally substituted. Examples of aryl include but not limited to phenyl, naphthyl or the like.
  • arylene when used alone or as part of a substituent group refers to an aryl diradical.
  • arylene refers to a mono-or bicyclic-aromatic hydrocarbon ring structure having 6 or 10 carbon atoms in the ring, wherein one or more of the carbon atoms in the ring is optionally substituted, and wherein the ring structure is directly attached to two other variable groups.
  • heteroaryl when used alone or as part of a substituent group, the term “heteroaryl” as used herein refers to a monocyclic aromatic ring that has at least one atom other than carbon in the ring, wherein the atom is selected from the group consisting of oxygen, nitrogen and sulfur. “Heteroaryl” also includes multicyclic ring systems that have at least one such aromatic ring. Thus, “heteroaryl” includes single aromatic rings of from about 1 to 6 carbon atoms and about 1-4 heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur. The sulfur and nitrogen atoms may also be present in an oxidized form provided the ring is aromatic.
  • Heteroaryl also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) wherein a heteroaryl group is condensed with one or more rings selected from the group consisting of heteroaryls or aryls.
  • a heteroaryl a single aromatic ring or multiple condensed ring system
  • a heteroaryl a monocyclic aromatic ring or multicyclic condensed ring system
  • the rings of a multi cyclic ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements.
  • heteroaryl refers to a mono-or bicyclic-aromatic ring structure including carbon atoms as well as up to four heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur.
  • heteroaryl rings can include a total of 5, 6, 9, or 10 ring atoms.
  • the heteroaryl moiety can be optionally substituted.
  • substituents include but not limited to halogen atoms; -C 1-3 alkyl groups, and C 1-3 haloalkyl groups. Halogen atoms include chlorine, fluorine, bromine, and iodine.
  • heteroarylene when used alone or as part of a substituent group refers to a heteroaryl diradical.
  • heteroarylene is a mono-or bicyclic-aromatic ring structure including carbon atoms as well as up to four heteroatoms selected from nitrogen, oxygen, and sulfur, wherein the ring structure is directly attached to two other variable groups.
  • alkoxy when used alone or as part of a substituent group refers to an oxygen radical attached to an alkyl group as defined herein by a single bond.
  • the alkoxy may be C 1- 6 alkoxy, e.g. C 1-4 alkoxy.
  • Examples of alkoxy groups include methoxy (-OCH 3 , ethoxy (-OCH 2 CH 3 , isopropoxy (-OCH (CH 3 ) 2 ) and the like.
  • haloalkoxy when used alone or as part of a substituent group refers to an oxygen radical attached to a haloalkyl group as defined herein by a single bond.
  • the haloalkoxy may be C 1-6 haloalkoxy, e.g. C 1-4 haloalkoxy.
  • Examples of haloalkoxy groups include -OCF 3 , -OCH 2 CF 3 , -OCH(CF 3 ) 2 , and the like.
  • amino represents -N(R) 2 , e.g, -NH 2 and -N(CH 3 ) 2 .
  • aminoalkyl refers to an amino group substituted by an alkyl group as defined herein.
  • the aminoalkyl may be C 1-6 aminoalkyl, e.g. C 1-4 aminoalkyl.
  • dialkylamino refers to an amino group substituted by two alkyl groups asdefined herein.
  • the alkyl groups in dialkylamino may be independently C 1-6 alkyl, eitheridentical or different.
  • halogen or “halo” refers to F, Cl, Br, or I.
  • cyano as used herein, represents a ⁇ CN group.
  • each R is, independently, any chemically feasible substituentdescribed herein.
  • haloacetyl refers to an acetyl group wherein at least one of thehydrogens has been replaced by a halogen.
  • hydroxy as used herein, represents a -OH group.
  • hydroxyalkyl represents an alkyl moiety substituted on one ormore carbon atoms with one or more -OH moieties.
  • sulfonyl as used herein, represents an -S(O) 2 - group.
  • vinyl ketone refers to a group comprising a carbonyl groupdirectly connected to a carbon-carbon double bond.
  • vinyl sulfone refers to a group comprising a sulfonyl groupdirected connected to a carbon-carbon double bond.
  • R is any chemically feasible substituent described herein.
  • C 1-3 includes C 1-3 , C 1-2 , C 2-3 , C 1 , C 2 , and C 3 .
  • C 1-6 alkyl when used alone or as part of a substituent group refers to an aliphatic linker having 1, 2, 3, 4, 5, or 6 carbon atoms and includes, for example, -CH 2 -, -CH (CH 3 ) -, -CH (CH 3 ) -CH 2 -, and -C (CH 3 ) 2 -
  • -C 0 alkyl- refers to a bond.
  • the C 1-6 alkyl can be substituted with one or more substituents.
  • the compounds described herein can be asymmetric (e.g., having one or more stereocenters) . All stereoisomers, such as enantiomers and diastereomers, are intended unless otherwise indicated.
  • Tautomeric forms result from the swapping of a single bond with an adjacent double bond together with the concomitant migration of a proton.
  • Tautomeric forms include prototropic tautomers which are isomeric protonation states having the same empirical formula and total charge.
  • Example prototropic tautomers include ketone -enol pairs, amide -imidic acid pairs, lactam -lactim pairs, amide -imidic acid pairs, enamine imine pairs, and annular forms where a proton can occupy two or more positions of a heterocyclic system, for example, 1H-and 3H-imidazole, 1H-, 2H-and 4H-1, 2, 4-triazole, 1H-and 2H-isoindole, and 1H-and 2H-pyrazole.
  • Tautomeric forms can be in equilibrium or sterically locked into one form by appropriate substitution.
  • the compounds of the present disclosure may exist as rotational isomers. In some embodiments, the compounds of the present disclosure exist as mixtures of rotational isomers in any proportion. In other embodiments, the compounds of the present disclosure exist as particular rotational isomers, substantially free of other rotational isomers.
  • Compounds of the disclosure can also include all isotopes of atoms occurring in the intermediates or final compounds.
  • Isotopes include those atoms having the same atomic number but different mass numbers.
  • isotopes of hydrogen include tritium and deuterium.
  • the compounds of the disclosure, and salts thereof are substantially isolated.
  • substantially isolated is meant that the compound is at least partially or substantially separated from the environment in which is formed or detected.
  • Partial separation can include, for example, a composition enriched in the compound of the disclosure.
  • Substantial separation can include compositions containing at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 97%, or at least about 99%by weight of the compound of the disclosure, or salt thereof.
  • phrases “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
  • the present disclosure also includes pharmaceutically acceptable salts of the compounds described herein.
  • pharmaceutically acceptable salts refers to derivatives of the disclosed compounds wherein the parent compound is modified by converting an existing acid or base moiety to its salt form.
  • examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like.
  • the pharmaceutically acceptable salts of the present disclosure include the conventional non-toxic salts of the parent compound formed, for example, from non-toxic inorganic or organic acids.
  • the pharmaceutically acceptable salts of the present disclosure can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods.
  • such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington ’s PharmaceuticaI Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, p. 1418 and Journal of Ph ⁇ rmaceutical Science, 66, 2 (1977) , each of which is incorporated herein by reference in its entirety.
  • a “pharmaceutically acceptable salt” refers to a salt of a compound of the disclosure that is pharmaceutically acceptable and that possesses the desired pharmacological activity of the parent compound.
  • such salts are non-toxic may be inorganic or organic acid addition salts and base addition salts.
  • such salts include: (1) acid addition salts, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or formed with organic acids such as acetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, 3- (4-hydroxybenzoyl) benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1, 2-ethane-disulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, 4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid, 4-toluenesulfonic acid,
  • Salts further include, by way of example only, sodium, potassium, calcium, magnesium, ammonium, tetraalkylammonium, and the like; and when the compound contains a basic functionality, salts of non-toxic organic or inorganic acids, such as hydrochloride, hydrobromide, tartrate, mesylate, acetate, maleate, oxalate and the like.
  • non-toxic organic or inorganic acids such as hydrochloride, hydrobromide, tartrate, mesylate, acetate, maleate, oxalate and the like.
  • a “pharmaceutically acceptable excipient” refers to a substance that is non-toxic, biologically tolerable, and otherwise biologically suitable for administration to a subject, such as an inert substance, added to a pharmacological composition or otherwise used as a vehicle, carrier, or diluent to facilitate administration of an agent and that is compatible therewith.
  • excipients include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils, and polyethylene glycols.
  • terapéuticaally effective amount refers to the amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue, system, animal, individual or human that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes one or more of the following: (1) Preventing the disease; for example, preventing a disease, condition or disorder in an individual that may be predisposed to the disease, condition or disorder but does not yet experience or display the pathology or symptomatology of the disease, (2) Inhibiting the disease; for example, inhibiting a disease, condition or disorder in an individual that is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., arresting further development of the pathology and/or symptomatology) , and (3) Ameliorating the disease; for example, ameliorating a disease, condition or disorder in an individual that is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., reversing the pathology and/or symptom
  • a “solvate” refers to a physical association of a compound of Formula I with one or more solvent molecules.
  • a “subject” refers to a mammal, particularly a human.
  • the terms “human, ” “patient, ” and “subject” are used interchangeably herein.
  • Treating” or “treatment” of any disease or disorder refers, in one embodiment, to ameliorating the disease or disorder (i.e., arresting or reducing the development of the disease or at least one of the clinical symptoms thereof) .
  • “treating” or “treatment” refers to ameliorating at least one physical parameter, which may not be discernible by the subject.
  • “treating” or “treatment” refers to modulating the disease or disorder, either physically, (e.g., stabilization of a discernible symptom) , physiologically, (e.g., stabilization of a physical parameter) , or both.
  • “treating” or “treatment” refers to delaying the onset of the disease or disorder.
  • Compounds of the present disclosure, ” and equivalent expressions are meant to embrace compounds of Formula I as described herein, as well as its subgenera for example, Formulae I-1 to I-12, which expression includes the stereoisomers (e.g., entaniomers, diastereomers) and constitutional isomers (e.g., tautomers) of compounds of Formula I as well as the pharmaceutically acceptable salts, where the context so permits.
  • stereoisomers e.g., entaniomers, diastereomers
  • constitutional isomers e.g., tautomers
  • isotopic variant refers to a compound that contains proportions of isotopes at one or more of the atoms that constitute such compound that is greater than natural abundance.
  • an “isotopic variant” of a compound can be radiolabeled, that is, contain one or more radioactive isotopes, or can be labeled with non-radioactive isotopes such as for example, deuterium ( 2 H or D) , carbon-13 ( 13 C) , nitrogen-15 ( 15 N) , or the like.
  • any hydrogen may be 2 H/D
  • any carbon may be 13 C
  • any nitrogen may be 15 N, and that the presence and placement of such atoms may be determined within the skill of the art.
  • proliferative disorder refers to a disorder or condition characterized by abnormal and uncontrolled cell proliferation. It may start at one site (primary site) with the potential to invade and to spread to other sites (secondary sites, metastases) which differentiate cancer (malignant tumor) from benign tumor. Virtually all the organs can be affected, leading to more than 100 types of cancer that can affect humans. Cancer is a typical proliferative disorder and can result from many causes including genetic predisposition, viral infection, exposure to ionizing radiation, exposure environmental pollutant, tobacco and or alcohol use, obesity, poor diet, lack of physical activity or any combination thereof.
  • isomers compounds that have the same molecular formula but differ in the nature or sequence of bonding of their atoms or the arrangement of their atoms in space are termed “isomers. ” Isomers that differ in the arrangement of their atoms in space are termed “stereoisomers, ” for example, diastereomers, enantiomers, and atrop-isomers.
  • the compounds of this disclosure may possess one or more asymmetric centers; such compounds can therefore be produced as individual (R) -or (S) -stereoisomers at each asymmetric center, or as mixtures thereof.
  • the compound according to the present disclosure may be present in unsolvated or solvated forms, including hydrate form.
  • the solvated forms are equivalent to unsolvated forms and both of them are encompassed within the scope of the present disclosure.
  • the present disclosure also encompasses any pharmaceutically acceptable derivative of the compounds according to formula (I) , e.g. ester, salt of the ester.
  • a particularly preferable derivative is prodrug. Upon administration to a subject, such a derivative can directly or indirectly provide the compound according to the present disclosure or its metabolite or residue with pharmaceutical activity.
  • a particularly preferable derivative e.g. prodrug
  • A is -N (H or CH 3 ) C (O) - (CH 2 ) -where the amino nitrogen is bound to the carbon atom of -C (R 1 R 2 ) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
  • B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • D is optionally substituted C 1 -C 4 alkylene, optionally substituted C 1 -C 4 alkenylene, optionally substituted C 1 -C 4 heteroalkylene, -C (O) O-CH (R 11 ) -where CH (R 11 ) -is bound to -C (R 5 R 6 ) -, -C (O) NH-CH (R 11 ) -where CH (R 11 ) -is bound to -C (R 5 R 6 ) -, optionally substituted C 1 -C4 heteroalkylene, or 3 to 8-membered heteroarylene;
  • L is absent or a linker
  • W is H, D, cyano, S (O) 2 R’ , an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1 -C 4 alkoxy, optionally substituted C 1 -C 4 hydroxyalkyl, optionally substituted C 1 -C 4 aminoalkyl, optionally substituted C 1 -C 4 haloalkyl, optionally substituted C 1 -C 4 alkyl, optionally substituted C 1 -C 4 guanidinoalkyl, C 0 -C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
  • X 1 is NR, O, S (O) n or CR 13 , n is 0, 1 or 2;
  • X 2 is O, NH or CR 14 ;
  • X 3 is O, NH or CR 14a ;
  • X 4 is N or CH
  • R is H, D, cyano, optionally substituted C 1 -C 4 alkyl, optionally substituted C 2 -C 4 alkenyl, optionally substituted C 2 -C 4 alkynyl, C (O) R’ , C (O) OR’, C (O) N (R’) 2 , S (O) R’, or S (O) 2 N (R’) 2 ; R’ is, independently, H, D or optionally substituted C 1 -C 4 alkyl;
  • R 1 is H, D, halo, hydroxy, C 1 -C 4 alkoxy, or C 1 -C 4 alkyl;
  • R 2 is H, D, halo, hydroxy, C 1 -C 4 alkoxy, or C 1 -C 4 alkyl;
  • R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1 -C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
  • R 5 is hydrogen, halogen, or optionally substituted C 1 -C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
  • R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1 -C 3 alkoxy, optionally substituted C 1 -C 3 alkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
  • R 5 ’ is hydrogen, halogen, or optionally substituted C 1 -C 3 alkyl
  • R 6 ’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1 -C 3 alkyl, optionally substituted C 2 -C 6 alkenyl, optionally substituted C 2 -C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10- membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5 ’ and R 6 ’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
  • R 7 is H, D, C 1 -C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1 -C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
  • R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1 -C 3 alkyl, optionally substituted C 1 -C 3 alkoxyl, or
  • R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
  • R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • R 10 is H, D, or C 1 -C 3 alkyl, cyclopropyl, cyclobutyl;
  • R 11 is hydrogen or methyl
  • R 12 is hydrogen, F, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
  • R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl
  • R 12’ is hydrogen or optionally substituted C 1 -C 6 alkyl
  • R 13 is H, D, cyano, optionally substituted C 1 -C 4 alkyl, optionally substituted C 1 -C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
  • R 14 is H, D, cyano, optionally substituted C 1 -C 3 alkyl, optionally substituted alkoxy;
  • R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
  • R 14a is H, D, cyano, optionally substituted C 1 -C 3 alkyl, optionally substituted alkoxy.
  • R 15 , R 16 , R 17 and R 18 are, independently, absent, H, D, CN, halogen, optionally substituted C 1 -C 3 alkyl, optionally substituted C 1 -C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl;
  • R 17 and R 18 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl;
  • R 19 is H, D, CN, Halogen, optionally substituted C 1 -C 6 alkyl, optionally substituted C 1 -C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 6 to 10-membered aryl, or optionally substituted 5 to 10-membered heteroaryl;
  • R 18 and R 19 combine with the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
  • Y 1 , Y 2 , Y 3 and Y 5 are, independently, C or N;
  • Y 4 is C, N, O or S.
  • Y 5 is C or N
  • R 20 is absent, H, D, optionally substituted C 1 -C 3 alkyl, optionally substituted C 1 -C 3 alkoxy, or R 18 and R 20 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl.
  • R 16 is selected from the group consisting of H, F, Cl, methyl, methoxy, vinyl, cyclopropyl, cyclobutyl or cyclopentyl.
  • R 18 is selected from methyl, ethyl, CF 3 CH 2 –.
  • R 19 is selected from:
  • Y 1 is C or N
  • R 15 is absent, H, D, CN, halogen, optionally substituted C 1 -C 3 alkyl, optionally substituted C 1 -C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl.
  • R 15 is selected from the group consisting of H, F, Cl, methyl, methoxy, vinyl, cyclopropyl.
  • A is -N (H or CH 3 ) C (O) - (CH 2 ) -where the amino nitrogen is bound to the carbon atom of -CH 2 -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene.
  • A is optionally substituted 6-membered arylene. In some embodiments, A has the structure:
  • R 21 is hydrogen, hydroxy, amino, cyano, optionally substituted C 1 -C 6 alkyl, or optianlly substituted C 1 -C 6 heteroalkyl.
  • A is selected from
  • E is –CHR 12 –.
  • R 12 is optionally substituted C 1 -C 6 alkyl or optionally substituted 3 to 6-membered cycloalkyl.
  • R 12 is:
  • L has the structure of Formula IIa:
  • n 0 or 1
  • R 22 is hydrogen or optionally substituted C 1 -C 6 alkyl
  • G is C (O) or SO 2 ;
  • Cy is optionally substituted 3 to 8 membered cycloalkylene, optionally substituted 3 to 8-membered heterocycloalkylene, optionally substituted 6-10 membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
  • L 1 is absent, -C (O) -, -SO 2 -, optionally substituted C 1 -C 4 alkylene or optionally substituted C 1 -C 4 heteroalkylene.
  • L has the structure:
  • W is H, D, cyano, S (O) 2 R’ , an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone.
  • W has the structure:
  • Z is H, D, CN, halogen, optionally substituted C 1 -C 4 alkyl, optionally substituted C 1 -C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl.
  • G is H, D, CN, halogen, optionally substituted C 1 -C 4 alkyl, optionally substituted C 1 -C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, OR 23 or NR 23 ;
  • R 23 is optionally substituted C 1 -C 4 alkyl, optionally substituted C 1 -C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene.
  • Cy 1 is optionally substituted 5 to 10-membered cycloalkenyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene.
  • Cy 2 is optionally substituted 5 to 10-membered cycloalkenyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene.
  • Cy 3 is optionally substituted 3 to 10-membered cycloalkenyl, optionally substituted 4 to 10-membered heterocycloalkyl.
  • R 24 is optionally substituted C 1 to C 4 alkyl, optionally substituted C 1 -C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl.
  • a compound of present invention or the pharmaceutically acceptable salt or stereoisomerthereof, wherein the compound is selected from:
  • a series of macrocyclic esters of formula 1-13 can be prepared by the methods outlined in Scheme 1.
  • Protecting the 3-hydroxy group of 1-1 generate 1-2.1-2 can be converted to boronic acid ester by using [Ir (OMe) (COD) ] 2 .
  • Hydrolysis the easter 1-3 with suitable base e.g., LiOH, NaOH
  • suitable base e.g., LiOH, NaOH
  • Compound 1-8 can be prepared by Suzuki reaction of 1-6 and 1-7. Suzuki reaction of 1-8 with boronic acid easter 1-5 can yield 1-9 which removed the protection of hydroxy and hydrolysis the ester to produce the precursor of macrocyclic ester 1-10.
  • Esterification of 1-10 by suitable coupling reagent e.g., EDCI/HOBt, HATU, DCC, Yamaguchi reagent
  • suitable coupling reagent e.g., EDCI/HOBt, HATU, DCC, Yamaguchi reagent
  • a series of macrocyclic esters can be prepared by the methods outlined in Scheme 2.
  • Compound 2-1 can be achieved by Suzuki coupling of indole derivative 1-8 with heteroaryl boronic acid or boronic ester 1-3.
  • Hydrolysis of the ester 2-1 by suitable base e.g., LiOH, NaOH
  • suitable base e.g., LiOH, NaOH
  • suitable base e.g., LiOH, NaOH
  • a series of macrocyclic esters can be prepared by the methods outlined in Scheme 3.
  • Compound 3-2 can be achieved by Suzuki coupling of indole derivative 3-1 with heteroaryl boronic acid or boronic ester 1-3 then hydrolysis.
  • 3-2 coupling with hexahydropyridazine compound to afford 3-3.
  • Through iodination of 3-3 produce 3-4.
  • Protect with 3-4 to give 3-5.3-5 can be converted to final compounds through the route in Scheme 1.
  • a series of macrocyclic esters can be prepared by the methods outlined in Scheme 4.
  • Compound 4-1 can be achieved by Miyaura boration using suitable catalyst (Pd (dppf) Cl 2 , Pd 2 (dba) 3 , Pd (PPh 3 ) 4 ) .
  • Compound 4-3 can be prepared by Suzuki reaction of 4-1 and 4-2. Removing the protecting group of the hydroxy and hydrolysis 4-3 can afford 4-4. Esterification of 4-4 to afford 4-5.4-5 can be converted to final compounds through the route in Scheme 1.
  • This assay is to measure the ability of the test compounds to inhibit K-Ras in cells. Activated K-Ras induces phosphorylation of ERK at Threonine 202 and Tyrosine 204 (pERK) . This procedure measures a decrease in cellular pERK in response to test compounds.
  • This protocol may be executed substituting other characterize inhibitors of other RAS variants, including, for example, NCI-H358 (K-Ras G12C) , HCT116 (K-Ras G13D) , LOVO (K-Ras G13D) , Mia-pa-ca2 (K-Ras G12C) .
  • cellular pERK level was determined using the ELISA
  • cellular pERK level was determined using HTRF cellular phospho- protein assays, Advanced phosphor-ERK, Phospho-ERK (CISBIO)
  • cellular pERK was determeined by Western.
  • Protocol Cell Viability Assay. The purpose of this cellular assay is to determine the effects of test compounds on the proliferation of three human cancer cell lines: NCI-H358 (K-Ras G12C) , HCT116 (K-Ras G13D) and Mia-pa-ca2 (K-Ras G12C) .

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Abstract

The present disclosure discloses a compound, or a pharmaceutically acceptable salt thereof having the structure of Formula (I), and pharmaceutical compositions including the same, that are inhibitors of one or more RAS proteins and are useful in the treatment of RAS-associated diseases such as cancer.

Description

MACROCYCLE COMPOUNDS AS INHIBITORS OF RAS TECHNICAL FIELD
The present disclosure relates to macrocycle compounds, and pharmaceutical compositions including the same, that are inhibitors of one or more RAS proteins and are useful in the treatment of RAS-associated diseases such as cancer.
BACKGROUND
Most small molecule drugs, as we know today, act by binding onto a pocket on a targeted protein, thereby influence the biological activities of that protein. There are many successful examples that resulted in marketed drugs that help millions of patients through the ingenious work of scientists for the past few decades. Despite all these successes, one ominous fact persistently remains is that most proteins are undruggable targets. Current estimates set targetable all human proteins at 10% (Bojadzic and Buchwald, Curr Top Med Chem 18: 674-699, 2019) . The other 90%human proteins remain as vast rich resources waiting to be explored. The discovery of allostery of proteins provides a great deal of possibilities to tap into that reservoir.
It's been fully demonstrated in literature that RAS proteins (K-Ras, H-Ras, N-Ras etc. ) play essential roles in various cancers. More importantly, various mutations in RAS proteins account for approximately 30%of all cancers studied. Among all RAS proteins, KRAS is the most frequently mutated RAS protein, and therefore is the most sought after target for cancer therapy. Fortunately, there is a small molecule drug inhibiting KRAS approved for clinical use after decade long extensive research. Considering that there are so many types of cancers related to KRAS proteins, it remains a clinical need for more small molecule drugs inhibiting KRAS proteins.
SUMMARY
The inventors believe that modulating the activity of a protein can be achieved via a direct binding with a synthetic ligand to this protein or by interacting with another intracellular protein which forms complexes with this protein. In the later cases, a high affinity three component  complex will be formed. The two intracellular proteins involved do not interact under normal conditions, but the addition of a synthetic ligand induces the formation of the three components conjugate which results in affecting the biological activity of the targeted protein. More specifically, the disclosed macrocyclic small molecules here are capable of perturbating biological processes through binding to a member of the cyclophilin A family (presenter protein) and a mutated RAS protein (target protein) .
In an aspect, provided is a compound of Formula (I) :
Figure PCTCN2021112499-appb-000001
or a pharmaceutically acceptable salt thereof, wherein:
A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally  substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
L is absent or a linker;
W is H, D, cyano, S (O)  2R’ , an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
X 1 is NR, O, S (O)  n or CR 13, n is 0, 1 or 2;
X 2 is O, NH or CR 14;
X 3 is O, NH or CR 14a;
X 4 is N or CH;
R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2; R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or  optionally substituted 6 to 10 membered aryl; or
R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6- membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
R 11 is hydrogen or methyl;
R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl;
R 13 is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy,  optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
R 14 is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy;
R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
R 14a is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy.
DETAILED DESCRIPTION
General definition
Unless stated otherwise, the terms and phrases used herein have the following meaning. A specific term or phrase shall not be considered as unclear or indefinite when it is not specifically defined. It should be understood according to the general meaning. The trade name used herein refers to the corresponding product or the active ingredient.
Unless otherwise defined hereinafter, all technical and scientific terms used herein have the same meaning as commonly understood by a person skilled in the art. The techniques used herein refer to those that are generally understood in the art, including the variants and equivalent substitutions that are obvious to those skilled in the art. While the following terms are believed to be readily comprehensible by a person skilled in the art, the following definitions are set forth to better illustrate the present disclosure. All patents, published patents applications, and publications cited herein are hereby incorporated by reference.
The present disclosure may be more fully appreciated by reference to the following description, including the following definitions and examples. Certain features of the disclosed compositions and methods which are described herein in the context of separate aspects, may also be provided in combination in a single aspect. Alternatively, various features of the disclosed compositions and methods that are, for brevity, described in the context of a single aspect, may also be provided separately or in any sub-combination.
When a certain amount, concentration, or other value or parameter is set forth in the form of a range, a preferred range, or a preferred upper limit or a preferred lower limit, it should be  understood that it is equivalent to specifically revealing any range formed by combining any upper limit or preferred value with any lower limit or preferred value, regardless of whether the said range is explicitly recited. Unless otherwise stated, the numerical ranges listed herein are intended to include the endpoints of the range and all integers and fractions (decimals) within the range. For example, the expression “about 0.01%to about 1%” means any values between 0.01%and 1%, for example 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%and 1%. Other similar expressions like “40%-50%to about 50%-70%” should also be understood in a similar manner.
Unless otherwise stated herein, singular forms like “a” and “the” include the plural forms. The expression “one or more” or “at least one” may mean 1, 2, 3, 4, 5, 6, 7, 8, 9 or more.
The terms “about” and “approximately” , when used in connection with a numerical variable, generally mean that the value of the variable and all values of the variable are within experimental error (for example, within a 95%confidence interval for the mean) or within ±10%of a specified value, or a wider range.
The term “mixture” is intended to mean a mixture containing more than one species of compounds, wherein one or more species of compounds can be target compound (s) . The term “target compound” means a compound to be separated or purified. When defining a separation process, the species of the target compound (s) are determined before the separation operations. It is to be understood that the product which contains the target compound (s) could be in any desired form, for example a product containing a pure isomer compound or a mixture containing a plurality of predefined species of the target compounds.
The term “optional” or “optionally” means the event described subsequent thereto may, but not necessarily happen, and the description includes the cases wherein the said event or circumstance happens or does not happen. Accordingly, the term “optionally substituted” means the given group may be substituted or unsubstituted. When substituted, the substituents of an “optionally substituted” group may include, without limitation, one or more substituents independently selected from the following groups or a particular designated set of groups, alone or in combination, as defined herein: alkyl, alkenyl, alkynyl, alkanoyl, heteroalkyl, heterocycloalkyl, haloalkyl, haloalkenyl, haloalkynyl, perhaloalkyl, perhaloalkoxy, cycloalkyl,  phenyl, aryl, aryloxy, alkoxy, haloalkoxy, oxo, acyloxy, carbonyl, carboxyl, alkylcarbonyl, carboxyester, carboxamido, cyano, hydrogen, halogen, hydroxy, amino, alkylamino, arylamino, amido, nitro, thiol, alkylthio, haloalkylthio, perhaloalkylthio, arylthio, sulfonate, sulfonic acid, tri substituted silyl, N 3, SH, SCH 3, C (O) CH 3, CO 2CH 3, CO 2H, pyridinyl, thiophene, furanyl, carbamate, and lower urea. Two substituents may be joined together to form a fused five-, six-, or seven-membered carbocyclic or heterocyclic ring comprising zero to three heteroatoms, for example forming methylenedioxy or ethylenedioxy. An optionally substituted group may be unsubstituted (e.g., -CH 2CH 3) , fully substituted (e.g., -CF 2CF 3) , mono-substituted (e.g., -CH 2CH 2F) or substituted at a level anywhere in-between fully substituted and mono-substituted (e.g., -CH 2CF 3) .
The expression “comprising” or similar expressions “including, ” “containing” and “having” are open-ended, and do not exclude additional unrecited elements, steps, or ingredients. The expression “consisting of” excludes any element, step, or ingredient not designated. The expression “consisting essentially of” means that the scope is limited to the designated elements, steps or ingredients, plus elements, steps or ingredients that are optionally present that do not substantially affect the essential and novel characteristics of the claimed subject matter. It should be understood that the expression “comprising” encompasses the expressions “consisting essentially of” and “consisting of” .
The chemical bond in the compound of the disclosure can be depicted herein with a solid line [——] , a wavy line
Figure PCTCN2021112499-appb-000002
asolid wedge
Figure PCTCN2021112499-appb-000003
or a dashed wedge
Figure PCTCN2021112499-appb-000004
It is intended that a bond to an asymmetric atom depicted with a solid line indicates that all possible stereoisomers at the atom (e.g., specific enantiomers, racemic mixtures and the like) are contemplated. It is intended that a bond to an asymmetric atom depicted with a wavy line indicates that the bond is either a solid wedge
Figure PCTCN2021112499-appb-000005
bond or a dashed wedge
Figure PCTCN2021112499-appb-000006
bond. It is intended that a bond to an asymmetric atom depicted with a solid or dashed wedge indicates the existence of the stereoisomer that is shown. When present in a racemic mixture, a solid or dashed wedge is used to define relative stereochemistry rather than absolute stereochemistry. Unless otherwise indicated, it is intended that the compound of the disclosure can be present in the form of stereoisomers (including cis-and trans-isomers, optical isomers (e.g., R and S enantiomers) , diastereomers, geometric isomers, rotamers, conformers, atropisomers, and  mixtures thereof) . The compound of the disclosure can exhibit one or more types of the above isomerism and can be consisted of a mixture thereof (e.g., a racemic mixture and/or a diastereomeric pair) .
It is further intended that the compounds of the present disclosure are stable. As used herein “stable” refers to a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and preferably capable of formulation into an efficacious therapeutic agent.
The term “alkyl” , when used alone or as part of a substituent group, refers to a straight-or branched-chain hydrocarbon group having from 1 to 6 carbons atoms ( “C 1-6” ) , in the group. Examples of alkyl groups include methyl (Me, C 1alkyl) , ethyl (Et, C 2alkyl) , n-propyl (C 3alkyl) , isopropyl (C 3alkyl) , butyl (C 4alkyl) , isobutyl (C 4alkyl) , sec-butyl (C 4alkyl) , tert-butyl (C 4alkyl) , pentyl (C 5alkyl) , isopentyl (C 5alkyl) , tert-pentyl (C 5alkyl) , hexyl (C 6alkyl) , isohexyl (C 6alkyl) , and the like.
The term “haloalkyl” , when used alone or as part of a substituent group, refers to a straight or branched-chain hydrocarbon group having from 1 to 6 carbons atoms ( “C 1-6” ) in the group, wherein one or more of the hydrogen atoms in the group have been replaced by a halogen atom. Examples of haloalkyl groups include trifluoromethyl (-CF 3, C 1haloalkyl) , trifluoroethyl (-CH 2CF 3, C 2haloalkyl) , and the like.
The term “alkylene” , when used alone or as part of a substituent group, refers to an alkyl diradical, i.e., a straight-or branched-chain hydrocarbon group having from 1 to 6 carbons atoms ( “C 1-6” ) , in the group, wherein the group is directly attached to two other variable groups.
The term “cycloalkyl” refers to non-aromatic cyclic hydrocarbons including cyclized alkyl and/or alkenyl groups. Cycloalkyl groups thus also encompass cycloalkenyl groups. Cycloalkyl groups can include mono-or polycyclic (e.g., having 2 or more than 2 fused rings) groups, spirocycles, and bridged rings (e.g., a bridged bicycloalkyl group) . Ring-forming carbon atoms of a cycloalkyl group can be optionally substituted by oxo or sulfido (e.g., C (O) or C (S) ) . Also included in the definition of cycloalkyl are moieties that have one or more aromatic rings fused (i.e., having a bond in common with) to the cycloalkyl ring, for example, benzo or thienyl derivatives of cyclopentane, cyclohexane, and the like. A cycloalkyl group containing a fused  aromatic ring can be attached through any ring-forming atom including a ring-forming atom of the fused aromatic ring. Cycloalkyl groups can have 3, 4, 5, 6, 7, 8, 9, or 10 ring-forming carbons (C 3-10) . In some embodiments, the cycloalkyl is a C 3-10 monocyclic or bicyclic cyclocalkyl. In some embodiments, the cycloalkyl is a C 3-10 monocyclic or bicyclic cycloalkyl which is optionally substituted by CH 2F, CHF 2, CF 3, and CF 2CF 3. In some embodiments, the cycloalkyl is a C 3-7 monocyclic cycloalkyl. In some embodiments, the cycloalkyl is a C 4-10 spirocycle or bridged cycloalkyl. Example cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclopentenyl, cyclohexenyl, cyclohexadienyl, cycloheptatrienyl, norbornyl, nopinyl, norcarnyl, cubane, adamantane, bicyclo [l. 1.1] pentyl, bicyclo [2. l. l] hexyl, bicyclo [2.2. l] heptanyl, bicyclo [3.1.1] heptanyl, bicyclo [2.2.2] octanyl, spiro [3 . 3] heptanyl, and the like. In some embodiments, cycloalkyl is cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl. In some embodiments, cycloalkyl are cyclic-containing, non-aromatic hydrocarbon groups having from 3 to 1 2 carbon atoms ( “C 3-12” ) , preferably from 3 to 6 carbon atoms ( “C 3-6” ) Examples of cycloalkyl groups include, for example, cyclopropyl (C 3; 3-membered) , cyclobutyl (C 4; 4-membered) , cyclopropylmethyl (C 4) , cyclopentyl (C 5) , cyclohexyl (C 6) , 1 -methylcyclopropyl (C 4) , 2-methylcyclopentyl (C 6) , adamantanyl (C 10) , and the like.
The term “cycloalkylene” , when used alone or as part of a substituent group refers to a cycloalkyl diradical, i.e., a cyclic-containing, non-aromatic hydrocarbon group having from 3 to 14 carbon atoms ( “C 3-14” ; or 3-14 membered) , for example 3 to 12 carbon atoms ( “C 3-12” ) , preferably from preferably from 3 to 7 carbon atoms ( “C 3-7” , or 3-7 membered) or 3 to 6 carbon atoms ( “C 3-6” ) , wherein the group is directly attached to two other variable groups. Cycloalkylene groups include spirocycloalkylene groups.
The term “cycloalkenylene” refers to a cycloalkenylene diradical.
The term “heteroalkylene” refers to a heteroalkylene diradical. The term “spirocycloalkyl” when used alone or as part of a substituent group refers to a non-aromatic hydrocarbon group containing two cycloalkyl rings, and wherein the two cycloalyl rings share a single carbon atom in common.
The term “spirocycloalkylene” when used alone or as part of a substituent group refers to  a spirocycloalkyl diradical, i.e., a non-aromatic hydrocarbon group containing two cycloalkyl rings, and wherein the two cycloalyl rings share a single carbon atom in common, and wherein the group is directly attached to two other variable groups.
The term “heterocycloalkyl” refers to monocyclic or polycyclic heterocycles having at least one non-aromatic ring (saturated or partially unsaturated ring) , wherein one or more of the ring-forming carbon atoms of the heterocycloalkyl is replaced by a heteroatom selected from the group consisting of N, O, S and B, and wherein the ring-forming carbon atoms and heteroatoms of the heterocycloalkyl group can be optionally substituted by one or more oxo or sulfido (e.g., C (O) , S (O) , C (S) , or S (O)  2, etc. ) . Heterocycloalkyl groups include monocyclic and polycyclic (e.g., having 2 or more than 2 fused rings) systems. Included in heterocycloalkyl are monocyclic and polycyclic 4-10, 4-7, and 3-14 membered heterocycloalkyl groups. Heterocycloalkyl groups can also include spirocycles and bridged rings (e.g., a 5-10 membered bridged biheterocycloalkyl ring having one or more of the ring-forming carbon atoms replaced by a heteroatom independently selected from the group consisting of N, O, S and B) . The heterocycloalkyl group can be attached through a ring-forming carbon atom or a ring-forming heteroatom. In some embodiments, the heterocycloalkyl group contains 0 to 3 double bonds. In some embodiments, the heterocycloalkyl group contains 0 to 2 double bonds.
Also included in the definition of heterocycloalkyl are moieties that have one or more aromatic rings fused (i.e., having a bond in common with) to the non-aromatic heterocyclic ring, for example, benzo or thienyl derivatives of piperidine, morpholine, azepine, etc. A heterocycloalkyl group containing a fused aromatic ring can be attached through any ring-forming atom including a ring-forming atom of the fused aromatic ring. In some embodiments, the heterocycloalkyl group contains 4 to 10 ring-forming atoms, 4 to 7 ring-forming atoms, or 3 to 14 ring-forming atoms. In some embodiments, the heterocycloalkyl group has 1 to 4 heteroatoms, 1 to 3 heteroatoms, 1 to 2 heteroatoms or 1 heteroatom. In some embodiments, the heterocycloalkyl is a monocyclic 4-6 membered heterocycloalkyl having 1 or 2 heteroatoms independently selected from the group consisting of N, O, S and B and having one or more oxidized ring members.
Example heterocycloalkyl groups include pyrrolidin-2-one, l, 3-isoxazolidin-2-one, pyranyl, tetrahydropyran, oxetanyl, azetidinyl, morpholino, thiomorpholino, piperazinyl,  tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, pyrrolidinyl, isoxazolidinyl, isothiazolidinyl, pyrazolidinyl, oxazolidinyl, thiazolidinyl, imidazolidinyl, azepanyl, benzazapene, 1, 2, 3, 4-tetrahydroisoquinoline, 2, 3-dihydrobenzofuryl, 1, 3-benzodioxole, benzo-1, 4-dioxane, azabicyclo [3.1.0] hexanyl, diazabicyclo [3.1.0] hexanyl, oxabicyclo [2. l. l] hexanyl, azabicyclo [2.2. l] heptanyl, diazabicyclo [2.2. l] heptanyl, azabicyclo [3.1.1] heptanyl, diazabicyclo [3.1.1] heptanyl, azabicyclo [3.2. l] octanyl, diazabicyclo [3.2.1] octanyl, oxabicyclo [2.2.2] octanyl, azabicyclo [2.2.2] octanyl, azaadamantanyl, diazaadamantanyl, oxa-adamantanyl, azaspiro [3.3] heptanyl, diazaspiro [3.3] heptanyl, oxa-azaspiro [3.3] heptanyl, azaspiro [3.4] octanyl, diazaspiro [3.4] octanyl, oxa-azaspiro [3.4] octanyl, azaspiro [2.5] octanyl, diazaspiro [2.5] octanyl, azaspiro [4.4] nonanyl, diazaspiro [4.4] nonanyl, oxa-azaspiro [4.4] nonanyl, azaspiro [4.5] decanyl, diazaspiro [4.5] decanyl, diazaspiro [4.4] nonanyl, oxa-diazaspiro [4.4] nonanyl and the like.
In some embodiments, heterocycloalkyl refers to any 3 to 10 membered monocyclic or bicyclic, saturated ring structure containing at least one heteroatom selected from the group consisting of O, N and S. The heterocycloalkyl group may be attached at any heteroatom or carbon atom of the ring such that the result is a stable structure. Examples of suitable heterocycloalkyl groups include, but are not limited to, azepanyl, aziridinyl, azetidinyl, pyrrolidinyl, dioxolanyl, imidazolidinyl, pyrazolidinyl, piperazinyl, piperidinyl, dioxanyl, morpholinyl, dithianyl, thiomorpholinyl, oxazepanyl, oxiranyl, oxetanyl, quinuclidinyl, tetrahydrofuranyl, tetrahydropyranyl, piperazinyl, and the like.
The term “heterocycloalkylene” , when used alone or as part of a substituent group refers to a heterocycloalkyl diradical. In some embodiments heterocycloalkylene any three to ten membered monocyclic or bicyclic, saturated ring structure containing at least one heteroatom selected from the group consisting of O, N and S, wherein the ring structure is directly attached to two other variable groups.
The term “alkenyl” when used alone or as part of a substituent group refers to a straight-or branched-chain group having from 2 to 6 carbon atoms ( “C 2-6” ) , preferably 2 to 4 carbons atoms ( “C 2-4” ) , in the group, wherein the group includes at least one carbon-carbon double bond.
Examples of alkenyl groups include vinyl (-CH=CH 2; C 2alkenyl) , allyl (-CH 2-CH=CH 2; C 3alkenyl) , propenyl (-CH=CHCH 3 C 3alkenyl) ; isopropenyl (-C (CH 3) =CH 2; C 3alkenyl) ,  butenyl (-CH=CHCH 2CH 3; C 4alkenyl) , sec-butenyl (-C (CH 3) =CHCH3; C 4alkenyl) , iso-butenyl (-CH=C (CH 3)  2; C 4alkenyl) , 2-butenyl (-CH 2CH=CHCH 3 C 4alkyl) , pentenyl (-CH=CHCH 2CH 2CH 3 or CH 2=CHCH 2CH 2CH 2-; C 5alkenyl) , and the like.
The term “alkenylene” , when used alone or as part of a substituent group refers to a alkenyl diradical, i.e., a straight-or branched-chain group having from 2 to 6 carbon atoms ( “C 2-6” ) , preferably 2 to 4 carbons atoms ( “C 2-4” ) , in the group, wherein the group includes at least one carbon-carbon double bond, and wherein the group is directly attached to two other variable groups.
The term “alkynyl” , when used alone or as part of a substituent group refers to a straight-or branched-chain group having from 2 to 6 carbon atoms ( “C 2-6” ) , preferably 2 to 4 carbons atoms ( “C 2-4” ) , in the group, wherein the group includes at least one carbon-carbon triple bond. Examples of alkynyl groups include ethynyl (-C≡CH; C 2alkynyl) , propragyl (-CH 2-CH≡CH; C 3alkynyl) , and the like.
The term “alkynylene” , when used alone or as part of a substituent group refers to an alkynyl diradical, i.e., a straight-or branched-chain group having from 2 to 12 carbon atoms ( “C 2-12” ) , preferably 2 to 4 carbons atoms ( “C 2-4” ) , in the group, wherein the group includes at least one carbon-carbon triple bond, and wherein the group is directly attached to two other variable groups.
The term “aryl” when used alone or as part of a substituent group refers to a monocyclic all carbon aromatic ring or a multicyclic all carbon ring system wherein the rings are aromatic. In some embodiments, “aryl” refers to a mono-or bicyclic-aromatic hydrocarbon ring structure having 6-10 carbon atoms in the ring, wherein one or more of the carbon atoms in the ring is optionally substituted. Examples of aryl include but not limited to phenyl, naphthyl or the like.
The term “arylene” when used alone or as part of a substituent group refers to an aryl diradical. In some embodiments, “arylene” refers to a mono-or bicyclic-aromatic hydrocarbon ring structure having 6 or 10 carbon atoms in the ring, wherein one or more of the carbon atoms in the ring is optionally substituted, and wherein the ring structure is directly attached to two other variable groups.
The term “heteroaryl” , when used alone or as part of a substituent group, the term “heteroaryl” as used herein refers to a monocyclic aromatic ring that has at least one atom other  than carbon in the ring, wherein the atom is selected from the group consisting of oxygen, nitrogen and sulfur. “Heteroaryl” also includes multicyclic ring systems that have at least one such aromatic ring. Thus, “heteroaryl” includes single aromatic rings of from about 1 to 6 carbon atoms and about 1-4 heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur. The sulfur and nitrogen atoms may also be present in an oxidized form provided the ring is aromatic. “Heteroaryl” also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) wherein a heteroaryl group is condensed with one or more rings selected from the group consisting of heteroaryls or aryls. Thus, a heteroaryl (a single aromatic ring or multiple condensed ring system) has about 1-20 carbon atoms and about 1-6 heteroatoms within the heteroaryl ring system. A heteroaryl (a monocyclic aromatic ring or multicyclic condensed ring system) can also have about 5 to 10 members within the heteroaryl ring. The rings of a multi cyclic ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements. In some embodiments, “heteroaryl” refers to a mono-or bicyclic-aromatic ring structure including carbon atoms as well as up to four heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur. In such embodiments, heteroaryl rings can include a total of 5, 6, 9, or 10 ring atoms. The heteroaryl moiety can be optionally substituted. Exemplary substituents include but not limited to halogen atoms; -C 1-3alkyl groups, and C 1-3haloalkyl groups. Halogen atoms include chlorine, fluorine, bromine, and iodine.
The term “heteroarylene” , when used alone or as part of a substituent group refers to a heteroaryl diradical. In some embodiments, heteroarylene is a mono-or bicyclic-aromatic ring structure including carbon atoms as well as up to four heteroatoms selected from nitrogen, oxygen, and sulfur, wherein the ring structure is directly attached to two other variable groups.
The term “oxo” refers to an oxygen substituent that is connected by a double bond (i.e., =O) .
The term “alkoxy” when used alone or as part of a substituent group refers to an oxygen radical attached to an alkyl group as defined herein by a single bond. The alkoxy may be C 1- 6alkoxy, e.g. C 1-4alkoxy. Examples of alkoxy groups include methoxy (-OCH 3, ethoxy (-OCH 2CH 3, isopropoxy (-OCH (CH 3)  2) and the like.
The term “haloalkoxy” when used alone or as part of a substituent group refers to an  oxygen radical attached to a haloalkyl group as defined herein by a single bond. The haloalkoxymay be C 1-6haloalkoxy, e.g. C 1-4haloalkoxy. Examples of haloalkoxy groups include -OCF 3, -OCH 2CF 3, -OCH(CF 3) 2, and the like.
The term “amino," as used herein, represents -N(R) 2, e.g, -NH 2 and -N(CH 3) 2.Theterm “aminoalkyl” refers to an amino group substituted by an alkyl group as defined herein.The aminoalkyl may be C 1-6 aminoalkyl, e.g. C 1-4 aminoalkyl.
The term “dialkylamino” refers to an amino group substituted by two alkyl groups asdefined herein. The alkyl groups in dialkylamino may be independently C 1-6 alkyl, eitheridentical or different.
The term “halogen” or “halo” refers to F, Cl, Br, or I.
The term "cyano" as used herein, represents a ―CN group.
The term “guanidinoalkyl" refers to a group having the structure:
Figure PCTCN2021112499-appb-000007
wherein each R is, independently, any chemically feasible substituentdescribed herein.
The term “haloacetyl" as used herein, refers to an acetyl group wherein at least one of thehydrogens has been replaced by a halogen.
The term "hydroxy" as used herein, represents a -OH group.
The term "hydroxyalkyl" as used herein, represents an alkyl moiety substituted on one ormore carbon atoms with one or more -OH moieties.
The term "sulfonyl" as used herein, represents an -S(O) 2- group.
The term "vinyl ketone” as used herein, refers to a group comprising a carbonyl groupdirectly connected to a carbon-carbon double bond.
The term "vinyl sulfone" as used herein, refers to a group comprising a sulfonyl groupdirected connected to a carbon-carbon double bond.
The term “ynone" as used herein, refers to a group comprising the structure:
Figure PCTCN2021112499-appb-000008
wherein R is any chemically feasible substituent described herein.
When a range of carbon atoms is used herein, for example, C 1-6, all ranges, as well as individual numbers of carbon atoms are encompassed. For example, “C 1-3” includes C 1-3, C 1-2, C 2-3, C 1, C 2, and C 3.
The term “C 1-6alkyl” when used alone or as part of a substituent group refers to an aliphatic linker having 1, 2, 3, 4, 5, or 6 carbon atoms and includes, for example, -CH 2-, -CH (CH 3) -, -CH (CH 3) -CH 2-, and -C (CH 3)  2-The term “-C 0alkyl-” refers to a bond. In some embodiments, the C 1-6alkyl can be substituted with one or more substituents.
The compounds described herein can be asymmetric (e.g., having one or more stereocenters) . All stereoisomers, such as enantiomers and diastereomers, are intended unless otherwise indicated. Compounds of the present disclosure that contain asymmetrically substituted carbon atoms can be isolated in optically active or racemic forms. Methods on how to prepare optically active forms from optically active starting materials are known in the art, such as by resolution of racemic mixtures or by stereoselective synthesis. Many geometric isomers of olefins, C=N double bonds, and the like can also be present in the compounds described herein, and all such stable isomers are contemplated in the present disclosure. Cis and trans geometric isomers of the compounds of the present disclosure are described and may be isolated as a mixture of isomers or as separated isomeric forms.
Compounds of the present disclosure also include tautomeric forms. Tautomeric forms result from the swapping of a single bond with an adjacent double bond together with the concomitant migration of a proton. Tautomeric forms include prototropic tautomers which are isomeric protonation states having the same empirical formula and total charge. Example prototropic tautomers include ketone -enol pairs, amide -imidic acid pairs, lactam -lactim pairs, amide -imidic acid pairs, enamine imine pairs, and annular forms where a proton can occupy two or more positions of a heterocyclic system, for example, 1H-and 3H-imidazole, 1H-, 2H-and 4H-1, 2, 4-triazole, 1H-and 2H-isoindole, and 1H-and 2H-pyrazole. Tautomeric forms can be in equilibrium or sterically locked into one form by appropriate substitution.
In some embodiments, the compounds of the present disclosure may exist as rotational isomers. In some embodiments, the compounds of the present disclosure exist as mixtures of rotational isomers in any proportion. In other embodiments, the compounds of the present disclosure exist as particular rotational isomers, substantially free of other rotational isomers.
Compounds of the disclosure can also include all isotopes of atoms occurring in the intermediates or final compounds. Isotopes include those atoms having the same atomic number but different mass numbers. For example, isotopes of hydrogen include tritium and deuterium.
In some embodiments, the compounds of the disclosure, and salts thereof, are substantially isolated. By “substantially isolated” is meant that the compound is at least partially or substantially separated from the environment in which is formed or detected. Partial separation can include, for example, a composition enriched in the compound of the disclosure. Substantial separation can include compositions containing at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 97%, or at least about 99%by weight of the compound of the disclosure, or salt thereof. Methods for isolating compounds and their salts are routine in the art.
The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
The present disclosure also includes pharmaceutically acceptable salts of the compounds described herein. As used herein, “pharmaceutically acceptable salts” refers to derivatives of the disclosed compounds wherein the parent compound is modified by converting an existing acid or base moiety to its salt form. Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. The pharmaceutically acceptable salts of the present disclosure include the conventional non-toxic salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. The pharmaceutically acceptable salts of the present disclosure can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods.  Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington ’s PharmaceuticaI Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, p. 1418 and Journal of Phαrmaceutical Science, 66, 2 (1977) , each of which is incorporated herein by reference in its entirety.
A “pharmaceutically acceptable salt” refers to a salt of a compound of the disclosure that is pharmaceutically acceptable and that possesses the desired pharmacological activity of the parent compound. In particular, such salts are non-toxic may be inorganic or organic acid addition salts and base addition salts. Specifically, such salts include: (1) acid addition salts, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or formed with organic acids such as acetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, 3- (4-hydroxybenzoyl) benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, 1, 2-ethane-disulfonic acid, 2-hydroxyethanesulfonic acid, benzenesulfonic acid, 4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid, 4-toluenesulfonic acid, camphorsulfonic acid, 4-methylbicyclo [2.2.2] -oct-2-ene-l-carboxylic acid, glucoheptonic acid, 3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylic acid, stearic acid, muconic acid, and the like; or (2) salts formed when an acidic proton present in the parent compound either is replaced by a metal ion, e.g., an alkali metal ion, an alkaline earth ion, or an aluminum ion; or coordinates with an organic base such as ethanolamine, diethanolamine, triethanolamine, N-methylglucamine and the like. Salts further include, by way of example only, sodium, potassium, calcium, magnesium, ammonium, tetraalkylammonium, and the like; and when the compound contains a basic functionality, salts of non-toxic organic or inorganic acids, such as hydrochloride, hydrobromide, tartrate, mesylate, acetate, maleate, oxalate and the like.
A “pharmaceutically acceptable excipient” refers to a substance that is non-toxic,  biologically tolerable, and otherwise biologically suitable for administration to a subject, such as an inert substance, added to a pharmacological composition or otherwise used as a vehicle, carrier, or diluent to facilitate administration of an agent and that is compatible therewith. Examples of excipients include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils, and polyethylene glycols.
The term “therapeutically effective amount” as used herein refers to the amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue, system, animal, individual or human that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes one or more of the following: (1) Preventing the disease; for example, preventing a disease, condition or disorder in an individual that may be predisposed to the disease, condition or disorder but does not yet experience or display the pathology or symptomatology of the disease, (2) Inhibiting the disease; for example, inhibiting a disease, condition or disorder in an individual that is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., arresting further development of the pathology and/or symptomatology) , and (3) Ameliorating the disease; for example, ameliorating a disease, condition or disorder in an individual that is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., reversing the pathology and/or symptomatology) .
A “solvate” refers to a physical association of a compound of Formula I with one or more solvent molecules.
A “subject” refers to a mammal, particularly a human. The terms “human, ” “patient, ” and “subject” are used interchangeably herein.
“Treating” or “treatment” of any disease or disorder refers, in one embodiment, to ameliorating the disease or disorder (i.e., arresting or reducing the development of the disease or at least one of the clinical symptoms thereof) . In another embodiment “treating” or “treatment” refers to ameliorating at least one physical parameter, which may not be discernible by the subject. In yet another embodiment, “treating” or “treatment” refers to modulating the disease or disorder, either physically, (e.g., stabilization of a discernible symptom) , physiologically, (e.g., stabilization of a physical parameter) , or both. In yet another embodiment, “treating” or “treatment” refers to delaying the onset of the disease or disorder.
“Compounds of the present disclosure, ” and equivalent expressions, are meant to embrace compounds of Formula I as described herein, as well as its subgenera for example, Formulae I-1 to I-12, which expression includes the stereoisomers (e.g., entaniomers, diastereomers) and constitutional isomers (e.g., tautomers) of compounds of Formula I as well as the pharmaceutically acceptable salts, where the context so permits.
As used herein, the term “isotopic variant” refers to a compound that contains proportions of isotopes at one or more of the atoms that constitute such compound that is greater than natural abundance. For example, an “isotopic variant” of a compound can be radiolabeled, that is, contain one or more radioactive isotopes, or can be labeled with non-radioactive isotopes such as for example, deuterium ( 2H or D) , carbon-13 ( 13C) , nitrogen-15 ( 15N) , or the like. It will be understood that, in a compound where such isotopic substitution is made, the following atoms, where present, may vary, so that for example, any hydrogen may be  2H/D, any carbon may be  13C, or any nitrogen may be  15N, and that the presence and placement of such atoms may be determined within the skill of the art.
The term “proliferative disorder” refers to a disorder or condition characterized by abnormal and uncontrolled cell proliferation. It may start at one site (primary site) with the potential to invade and to spread to other sites (secondary sites, metastases) which differentiate cancer (malignant tumor) from benign tumor. Virtually all the organs can be affected, leading to more than 100 types of cancer that can affect humans. Cancer is a typical proliferative disorder and can result from many causes including genetic predisposition, viral infection, exposure to ionizing radiation, exposure environmental pollutant, tobacco and or alcohol use, obesity, poor diet, lack of physical activity or any combination thereof.
It is also to be understood that compounds that have the same molecular formula but differ in the nature or sequence of bonding of their atoms or the arrangement of their atoms in space are termed “isomers. ” Isomers that differ in the arrangement of their atoms in space are termed “stereoisomers, ” for example, diastereomers, enantiomers, and atrop-isomers. The compounds of this disclosure may possess one or more asymmetric centers; such compounds can therefore be produced as individual (R) -or (S) -stereoisomers at each asymmetric center, or as mixtures thereof. Unless indicated otherwise, the description or naming of a particular compound in the specification and claims is intended to include all stereoisomers and mixtures, racemic or  otherwise, thereof. Where one chiral center exists in a structure, but no specific stereochemistry is shown for that center, both enantiomers, individually or as a mixture of enantiomers, are encompassed by that structure. Where more than one chiral center exists in a structure, but no specific stereochemistry is shown for the centers, all enantiomers and diastereomers, individually or as a mixture, are encompassed by that structure. The methods for the determination of stereochemistry and the separation of stereoisomers are well-known in the art.
The compound according to the present disclosure may be present in unsolvated or solvated forms, including hydrate form. In general, the solvated forms are equivalent to unsolvated forms and both of them are encompassed within the scope of the present disclosure.
The present disclosure also encompasses any pharmaceutically acceptable derivative of the compounds according to formula (I) , e.g. ester, salt of the ester. A particularly preferable derivative is prodrug. Upon administration to a subject, such a derivative can directly or indirectly provide the compound according to the present disclosure or its metabolite or residue with pharmaceutical activity. A particularly preferable derivative (e.g. prodrug) is the compound, which upon administration to a subject, will increase bioavailability of the compound according to the present disclosure or improve delivery of the parent compound to the tissues or organs of a living body.
The compound ofFormula I
In an aspect, provided is a compound of Formula (I) :
Figure PCTCN2021112499-appb-000009
or a pharmaceutically acceptable salt thereof, wherein:
A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally  substituted 5 to 10-membered heteroarylene;
B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
L is absent or a linker;
W is H, D, cyano, S (O)  2R’ , an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
X 1 is NR, O, S (O)  n or CR 13, n is 0, 1 or 2;
X 2 is O, NH or CR 14;
X 3 is O, NH or CR 14a;
X 4 is N or CH;
R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’ , C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2; R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6- membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10- membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered  heterocycloalkyl; or
R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
R 11 is hydrogen or methyl;
R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl;
R 13 is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
R 14 is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy;
R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
R 14a is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-1) ,
Figure PCTCN2021112499-appb-000010
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) herein.
R 15, R 16, R 17 and R 18 are, independently, absent, H, D, CN, halogen, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl;
or R 17 and R 18 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl;
R 19 is H, D, CN, Halogen, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 6 to 10-membered aryl, or optionally substituted 5 to 10-membered heteroaryl;
or R 18 and R 19 combine with the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
Y 1, Y 2, Y 3 and Y 5 are, independently, C or N;
Y 4 is C, N, O or S.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-2) ,
Figure PCTCN2021112499-appb-000011
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) and Formula (I-1) .
In an embodiment, provided is a compound of Formula (I) which has Formula (I-3)
Figure PCTCN2021112499-appb-000012
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) and Formula (I-1) .
Y 5 is C or N;
R 20 is absent, H, D, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, or R 18 and R 20 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-4)
Figure PCTCN2021112499-appb-000013
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) and Formula (I-3) .
In an embodiment, provided is a compound of Formula (I) which has Formula (I-5)
Figure PCTCN2021112499-appb-000014
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) and Formula (I-4) .
In a specific embodiment of Formula (I-5) , R 16 is selected from the group consisting of H, F, Cl, methyl, methoxy, vinyl, cyclopropyl, cyclobutyl or cyclopentyl.
In a specific embodiment of Formula (I-5) , R 18 is selected from methyl, ethyl, CF 3CH 2–.
In a specific embodiment of Formula (I-5) , R 19 is selected from:
Figure PCTCN2021112499-appb-000015
In an embodiment, provided is a compound of Formula (I) which has Formula (I-6)
Figure PCTCN2021112499-appb-000016
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) , Formula (I-4) and Formula (I-5) .
Y 1 is C or N;
R 15 is absent, H, D, CN, halogen, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl.
In a specific embodiment of Formula (I-6) , R 15 is selected from the group consisting of H, F, Cl, methyl, methoxy, vinyl, cyclopropyl.
A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -CH 2 -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene.
In some embodiment, A is optionally substituted 6-membered arylene. In some embodiments, A has the structure:
Figure PCTCN2021112499-appb-000017
Wherein R 21 is hydrogen, hydroxy, amino, cyano, optionally substituted C 1-C 6 alkyl, or optianlly substituted C 1-C 6 heteroalkyl.
In some embodiment, A is selected from
Figure PCTCN2021112499-appb-000018
Figure PCTCN2021112499-appb-000019
In some embodiments of the present invention, E is –CHR 12–. In some embodiments, R 12 is optionally substituted C 1-C 6 alkyl or optionally substituted 3 to 6-membered cycloalkyl. In some embodiments, R 12 is:
Figure PCTCN2021112499-appb-000020
In some embodiments, L has the structure of Formula IIa:
Figure PCTCN2021112499-appb-000021
Wherein m is 0 or 1;
R 22 is hydrogen or optionally substituted C 1-C 6 alkyl;
G is C (O) or SO 2;
Cy is optionally substituted 3 to 8 membered cycloalkylene, optionally substituted 3 to 8-membered heterocycloalkylene, optionally substituted 6-10 membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
L 1 is absent, -C (O) -, -SO 2-, optionally substituted C 1-C 4 alkylene or optionally substituted C 1-C 4 heteroalkylene. In some embodiments, L has the structure:
Figure PCTCN2021112499-appb-000022
In some embodiments, W is H, D, cyano, S (O)  2R’ , an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone. In some embodiments, W has the structure:
Figure PCTCN2021112499-appb-000023
Figure PCTCN2021112499-appb-000024
In an embodiment, provided is a compound of Formula (I) which has Formula (I-7)
Figure PCTCN2021112499-appb-000025
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) and Formula (I-4) .
Z is H, D, CN, halogen, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-8) 
Figure PCTCN2021112499-appb-000026
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) and Formula (I-4) .
G is H, D, CN, halogen, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, OR 23 or NR 23;
R 23 is optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to  10-membered heteroarylene.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-9) 
Figure PCTCN2021112499-appb-000027
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) and Formula (I-4) .
Cy 1 is optionally substituted 5 to 10-membered cycloalkenyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-10) 
Figure PCTCN2021112499-appb-000028
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) and Formula (I-4) .
Cy 2 is optionally substituted 5 to 10-membered cycloalkenyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-11)
Figure PCTCN2021112499-appb-000029
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) and Formula (I-4) .
Cy 3 is optionally substituted 3 to 10-membered cycloalkenyl, optionally substituted 4 to 10-membered heterocycloalkyl.
In an embodiment, provided is a compound of Formula (I) which has Formula (I-12)
Figure PCTCN2021112499-appb-000030
or a pharmaceutically acceptable salt thereof, wherein each of the groups are as defined in Formula (I) , Formula (I-2) , Formula (I-3) and Formula (I-4) .
R 24 is optionally substituted C 1 to C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl.
In some embodiments, a compound of present invention, or the pharmaceutically acceptable salt or stereoisomerthereof, wherein the compound is selected from:
Figure PCTCN2021112499-appb-000031
Figure PCTCN2021112499-appb-000032
Figure PCTCN2021112499-appb-000033
Figure PCTCN2021112499-appb-000034
Figure PCTCN2021112499-appb-000035
Figure PCTCN2021112499-appb-000036
Figure PCTCN2021112499-appb-000037
Figure PCTCN2021112499-appb-000038
Figure PCTCN2021112499-appb-000039
EXAMPLES
Synthesis Examples
Compounds of the present disclosure can be prepared according to numerous preparatory routes known in the literature. The Schemes below provide general guidance in connection with preparing the compounds of the present disclosure. One skilled in the art would understand that the preparations shown in the Schemes can be modified or optimized using general knowledge of organic chemistry to prepare various compounds of the present disclosure. Example synthetic methods for preparing compounds of the present disclosure are provided in the Schemes below.
A series of macrocyclic esters of formula 1-13 can be prepared by the methods outlined in Scheme 1. Protecting the 3-hydroxy group of 1-1 generate 1-2.1-2 can be converted to boronic acid ester by using [Ir (OMe) (COD) ]  2. Hydrolysis the easter 1-3 with suitable base (e.g., LiOH, NaOH) can afford the corresponding acid 1-4 which can be converted to 1-5 by coupling with the corresponding hexahydropyridazine compound. Compound 1-8 can be prepared by Suzuki reaction of 1-6 and 1-7. Suzuki reaction of 1-8 with boronic acid easter 1-5 can yield 1-9 which removed the protection of hydroxy and hydrolysis the ester to produce the precursor of macrocyclic ester 1-10. Esterification of 1-10 by suitable coupling reagent (e.g., EDCI/HOBt, HATU, DCC, Yamaguchi reagent) can give the macrocyclic ester 1-11. By removing the protecting group of 1-11 and coupling with corresponding acid can produce 1-13.
Scheme 1: General route to synthesize the macrocyclic esters
Figure PCTCN2021112499-appb-000040
Alternatively, a series of macrocyclic esters can be prepared by the methods outlined in  Scheme 2. Compound 2-1 can be achieved by Suzuki coupling of indole derivative 1-8 with heteroaryl boronic acid or boronic ester 1-3. Hydrolysis of the ester 2-1 by suitable base (e.g., LiOH, NaOH) can afford the corresponding acid 2-2 which coupling with hexahydropyridazine compound to afford 1-9.1-9 can be converted to final compounds through the route in Scheme 1.
Scheme 2: General route to synthesize the macrocyclic esters
Figure PCTCN2021112499-appb-000041
Alternatively, a series of macrocyclic esters can be prepared by the methods outlined in Scheme 3. Compound 3-2 can be achieved by Suzuki coupling of indole derivative 3-1 with heteroaryl boronic acid or boronic ester 1-3 then hydrolysis. 3-2 coupling with hexahydropyridazine compound to afford 3-3. Through iodination of 3-3 produce 3-4. Protect with 3-4 to give 3-5.3-5 can be converted to final compounds through the route in Scheme 1.
Scheme 3: General route to synthesize the macrocyclic esters
Figure PCTCN2021112499-appb-000042
A series of macrocyclic esters can be prepared by the methods outlined in Scheme 4. Compound 4-1 can be achieved by Miyaura boration using suitable catalyst (Pd (dppf) Cl 2, Pd 2(dba)  3, Pd (PPh 3)  4) . Compound 4-3 can be prepared by Suzuki reaction of 4-1 and 4-2. Removing the protecting group of the hydroxy and hydrolysis 4-3 can afford 4-4. Esterification of 4-4 to afford 4-5.4-5 can be converted to final compounds through the route in Scheme 1.
Scheme 4: General route to synthesize the macrocyclic esters
Figure PCTCN2021112499-appb-000043
Biological Assays
The purpose of this assay is to measure the ability of the test compounds to inhibit K-Ras in cells. Activated K-Ras induces phosphorylation of ERK at Threonine 202 and Tyrosine 204 (pERK) . This procedure measures a decrease in cellular pERK in response to test compounds. Note: This protocol may be executed substituting other characterize inhibitors of other RAS variants, including, for example, NCI-H358 (K-Ras G12C) , HCT116 (K-Ras G13D) , LOVO (K-Ras G13D) , Mia-pa-ca2 (K-Ras G12C) .
In some experiments, cellular pERK level was determined using the ELISA
In some experiments, cellular pERK level was determined using HTRF cellular phospho- protein assays, Advanced phosphor-ERK, Phospho-ERK (CISBIO)
In some experiments, cellular pERK was determeined by Western.
Determination ofCell Viability in RAS Mutant Cancer Cell Lines
Protocol: 
Figure PCTCN2021112499-appb-000044
Cell Viability Assay. The purpose of this cellular assay is to determine the effects of test compounds on the proliferation of three human cancer cell lines: NCI-H358 (K-Ras G12C) , HCT116 (K-Ras G13D) and Mia-pa-ca2 (K-Ras G12C) .

Claims (28)

  1. A compound, or a pharmaceutically acceptable salt thereof having the structure of Formula (I) :
    Figure PCTCN2021112499-appb-100001
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’ , an optionally substituted vinyl ketone, a vinyl sulfone, an optionally  substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    X 1 is NR, O, S (O)  n or CR 13, n is 0, 1 or 2;
    X 2 is O, NH or CR 14;
    X 3 is O, NH or CR 14a;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl;
    R 13 is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    R 14 is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy;
    R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 14a is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy.
  2. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-1) :
    Figure PCTCN2021112499-appb-100002
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    X 1 is NR, O, S (O)  n or CR 13, n is 0, 1 or 2;
    X 2 is O, NH or CR 14;
    X 3 is O, NH or CR 14a;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6  alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl;
    R 13 is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    R 14 is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy;
    R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene.
    R 14a is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy.
    R 15, R 16, R 17 and R 18 are, independently, absent, H, D, CN, halogen, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl;
    or R 17 and R 18 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl;
    R 19 is H, D, CN, Halogen, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 6 to 10-membered aryl, or optionally substituted 5 to 10-membered heteroaryl;
    or R 18 and R 19 combine with the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    Y 1, Y 2, Y 3 and Y 5 are, independently, C or N;
    Y 4 is C, N, O or S.
  3. A compound, or a pharmaceutically acceptable salt thereof according to any one of claims 1-2, having the structure of Formula (I-2) :
    Figure PCTCN2021112499-appb-100003
    wherein each of the groups are as defined in claims 1-2.
  4. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-3) :
    Figure PCTCN2021112499-appb-100004
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    X 1 is NR, O, S (O)  n or CR 13, n is 0, 1 or 2;
    X 2 is O, NH or CR 14;
    X 3 is O, NH or CR 14a;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6  alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl;
    R 13 is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    R 14 is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy;
    R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 14a is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy.
    R 15, R 16, R 17 and R 18 are, independently, absent, H, D, CN, halogen, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl;
    or R 17 and R 18 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl;
    R 19 is H, D, CN, Halogen, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 6 to 10-membered aryl, or optionally substituted 5 to 10-membered heteroaryl;
    or R 18 and R 19 combine with the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 20 is absent, H, D, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, or R 18 and R 20 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl;
    Y 1, Y 2, Y 3 and Y 5 are, independently, C or N;
    Y 4 is C, N, O or S.
  5. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-4) :
    Figure PCTCN2021112499-appb-100005
    wherein each of the groups are as defined in claim 4.
  6. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-5) :
    Figure PCTCN2021112499-appb-100006
    wherein each of the groups are as defined in claim 3.
  7. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-6) :
    Figure PCTCN2021112499-appb-100007
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    X 1 is NR, O, S (O)  n or CR 13, n is 0, 1 or 2;
    X 2 is O, NH or CR 14;
    X 3 is O, NH or CR 14a;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl;
    or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered  cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl;
    R 13 is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    R 14 is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy;
    R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 14a is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy.
    R 15 is absent, H, D, CN, halogen, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl;
    R 18 is independently, absent, H, D, CN, halogen, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl, optionally substituted 3 to 6-member cycloalkyl, optionally substituted 3 to 6-membered heterocycloalkyl;
    or R 17 and R 18 combine with the atoms to which they are attached to form an optionally substituted 5 to 14-membered heterocycloalkyl;
    R 19 is H, D, CN, Halogen, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 6 to 10-membered aryl, or optionally substituted 5 to 10-membered heteroaryl;
    or R 18 and R 19 combine with the atoms to which they are attached to form an optionally  substituted 3 to 14-membered heterocycloalkyl;
    Y 1 is C or N.
  8. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-7) :
    Figure PCTCN2021112499-appb-100008
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    Z is H, D, CN, halogen, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’;  C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl.
  9. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-8) :
    Figure PCTCN2021112499-appb-100009
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4  hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    G is H, D, CN, halogen, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, OR 23 or NR 23;
    R 23 is optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’;  C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine R 10 with the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl.
  10. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-9) :
    Figure PCTCN2021112499-appb-100010
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl,  optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    Cy 1 is optionally substituted 5 to 10-membered cycloalkenyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl,  optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl.
  11. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-10) :
    Figure PCTCN2021112499-appb-100011
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4  hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    Cy 2 is optionally substituted 5 to 10-membered cycloalkenyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl,  optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl.
  12. A compound, or a pharmaceutically acceptable salt thereof according to claim 1, having the structure of Formula (I-11) :
    Figure PCTCN2021112499-appb-100012
    wherein:
    A is -N (H or CH 3) C (O) - (CH 2) -where the amino nitrogen is bound to the carbon atom of -C (R 1R 2) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or optionally substituted 5 to 10-membered heteroarylene;
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl,  optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    Cy 3 is optionally substituted 3 to 10-membered cycloalkenyl, optionally substituted 4 to 10-membered heterocycloalkyl;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10- membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl.
  13. A compound, or a pharmaceutically acceptable salt thereof having the structure of Formula (I-12) :
    Figure PCTCN2021112499-appb-100013
    wherein:
    B is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    C is optionally substituted 5 to 6-membered cycloalkylene, optionally substituted 5 to 6-membered heterocycloalkylene, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    D is optionally substituted C 1-C 4 alkylene, optionally substituted C 1-C 4 alkenylene, optionally substituted C 1-C 4 heteroalkylene, -C (O) O-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, -C (O) NH-CH (R 11) -where CH (R 11) -is bound to -C (R 5R 6) -, optionally substituted C 1-C 4 heteroalkylene, or 3 to 8-membered heteroarylene;
    E is absent, -CH (R 12) -, or >C=CR 12R 12’ where the carbon is bound to the carbonyl carbon of -N (R 10) -C (O) -, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    L is absent or a linker;
    W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone, optionally substituted amino, optionally substituted amido, optionally substituted C 1-C 4 alkoxy, optionally substituted C 1-C 4 hydroxyalkyl, optionally substituted C 1-C 4 aminoalkyl, optionally substituted C 1-C 4 haloalkyl, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 guanidinoalkyl, C 0-C 4 alkyl optionally substituted 3 to 11-membered heterocycloalkyl, optionally substituted 3 to 8-membered cycloalkyl, or optionally substituted 3 to 8-membered heteroaryl;
    X 1 is NR, O, S (O)  n or CR 13, n is 0, 1 or 2;
    X 2 is O, NH or CR 14;
    X 3 is O, NH or CR 14a;
    X 4 is N or CH;
    R is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 2-C 4 alkenyl, optionally substituted C 2-C 4 alkynyl, C (O) R’, C (O) OR’, C (O) N (R’)  2, S (O) R’, or S (O)  2N (R’)  2;
    R’ is, independently, H, D or optionally substituted C 1-C 4 alkyl;
    R 1 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 2 is H, D, halo, hydroxy, C 1-C 4 alkoxy, or C 1-C 4 alkyl;
    R 3 and R 4 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, or combine with the carbon to which they are attached to form a carbonyl, or combine with the carbon to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5 is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl, or R 5 and R 11 combine with the carbon atoms to which they are attached to form an optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 6 is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkoxy, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10 membered aryl; or
    R 5 and R 6 combine with the carbon atom to which they are attached to form C=CR 5’R 6’; C=N (OH) , C=N (O-C 1-C 3 alkyl) , C=O, C=S, C=NH, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 5’ is hydrogen, halogen, or optionally substituted C 1-C 3 alkyl; R 6’ is hydrogen, halogen, hydroxy, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted C 2-C 6 alkenyl, optionally substituted C 2-C 6 alkynyl, optionally substituted 3 to 8-membered cycloalkyl, optionally substituted 3 to 14-membered heterocycloalkyl, optionally substituted 5 to 10-membered heteroaryl, or optionally substituted 6 to 10-membered aryl, or R 5’ and R 6’ combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl;
    R 7 is H, D, C 1-C 4 alkyl optionally substituted with halogen, cyano, hydroxy, or C 1-C 4 alkoxy, cyclopropyl, cyclobutyl or cyclopentyl;
    R 8 and R 9 are, independently, H, D, halo, optionally substituted C 1-C 3 alkyl, optionally substituted C 1-C 3 alkoxyl, or
    R 8 and R 9 combine with the carbon atom to which they are attached to form optionally substituted 3 to 6-membered cycloalkyl or optionally substituted 3 to 7-membered heterocycloalkyl; or
    R 9 and R 13 combine with the carbon atom to form optionally substituted 3 to 7-membered cycloalkyl, optionally substituted 3 to 7-membered heterocycloalkyl, optionally substituted 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 10 is H, D, or C 1-C 3 alkyl, cyclopropyl, cyclobutyl;
    R 11 is hydrogen or methyl;
    R 12 is hydrogen, F, optionally substituted C 1-C 6 alkyl, optionally substituted C 1-C 6 heteroalkyl, optionally substituted 3 to 6-membered cycloalkyl, or optionally substituted 3 to -7-membered heterocycloalkyl; or
    R 12 combine with R 10 the atoms to which they are attached to form an optionally substituted 3 to 14-membered heterocycloalkyl;
    R 12’ is hydrogen or optionally substituted C 1-C 6 alkyl;
    R 13 is H, D, cyano, optionally substituted C 1-C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkylene, optionally substituted 3 to 6-membered heterocycloalkylene, optionally substituted 6-membered arylene, or 5 to 6-membered heteroarylene;
    R 14 is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy;
    R 14 and R 13 combine with the carbon atom to form optionally substituted 5 to 7-membered cycloalkyl, optionally substituted 5 to 10-membered heterocycloalkyl, optionally substituted 5 to 6-membered arylene, or optionally substituted 5 to 6-membered heteroarylene;
    R 14a is H, D, cyano, optionally substituted C 1-C 3 alkyl, optionally substituted alkoxy.
    R 24 is optionally substituted C 1 to C 4 alkyl, optionally substituted C 1-C 4 alkoxy, optionally substituted 3 to 6-membered cycloalkenyl, optionally substituted 3 to 6-membered heterocycloalkyl.
  14. A compound, or a pharmaceutically acceptable salt thereof according to claim 6, wherein R 16 is selected from the group consisting of H, F, Cl, methyl, methoxy, vinyl, cyclopropyl, cyclobutyl or cyclopentyl; preferably, R 16 is selected from the group consisting of H, F, Cl, methyl, methoxy.
  15. A compound, or a pharmaceutically acceptable salt thereof according to claim 6, wherein  R 18 is selected from methyl, ethyl, CF 3CH 2–; preferably, R 18 is ethyl.
  16. A compound, or a pharmaceutically acceptable salt thereof according to claim 6, wherein R 19 is selected from
    Figure PCTCN2021112499-appb-100014
    Figure PCTCN2021112499-appb-100015
    Figure PCTCN2021112499-appb-100016
    preferably, R 19 is selected from
    Figure PCTCN2021112499-appb-100017
  17. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein A is optionally substituted 6-membered arylene.
  18. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein A has the structure:
    Figure PCTCN2021112499-appb-100018
    Wherein R 21 is hydrogen, hydroxy, amino, cyano, optionally substituted C 1-C 6 alkyl, or optianlly substituted C 1-C 6 heteroalkyl; preferably, R 21 is hydroxy.
  19. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein A is selected from
    Figure PCTCN2021112499-appb-100019
    Figure PCTCN2021112499-appb-100020
    preferably, A is
    Figure PCTCN2021112499-appb-100021
  20. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein E is –CHR 12–, wherein R 12 is optionally substituted C 1-C 6 alkyl or optionally substituted 3 to 6-membered cycloalkyl; preferably, R 12 is selected from:
    Figure PCTCN2021112499-appb-100022
    more preferably, R 12 is
    Figure PCTCN2021112499-appb-100023
  21. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein L has the structure of Formula IIa:
    Figure PCTCN2021112499-appb-100024
    Formula IIa
    Wherein m is 0 or 1;
    R 22 is hydrogen or optionally substituted C 1-C 6 alkyl;
    G is C (O) or SO 2;
    Cy is optionally substituted 3 to 8 membered cycloalkylene, optionally substituted 3 to 8-membered heterocycloalkylene, optionally substituted 6-10 membered arylene, or optionally substituted 5 to 10-membered heteroarylene; L 1 is absent, -C (O) -, -SO 2-, optionally substituted C 1-C 4 alkylene or optionally substituted C 1-C 4 heteroalkylene.
  22. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein L has the structure:
    Figure PCTCN2021112499-appb-100025
    Figure PCTCN2021112499-appb-100026
    preferably,
    Figure PCTCN2021112499-appb-100027
  23. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein W is H, D, cyano, S (O)  2R’, an optionally substituted vinyl ketone, a vinyl sulfone, an optionally substituted ynone, a haloacetal, an alkynyl sulfone.
  24. A compound, or a pharmaceutically acceptable salt thereof according to claim 7, wherein W has the structure:
    Figure PCTCN2021112499-appb-100028
    preferably, 
    Figure PCTCN2021112499-appb-100029
    Figure PCTCN2021112499-appb-100030
  25. A compound, or a pharmaceutically acceptable salt or stereoisomerthereof, wherein the compound is selected from:
    Figure PCTCN2021112499-appb-100031
    Figure PCTCN2021112499-appb-100032
    Figure PCTCN2021112499-appb-100033
    Figure PCTCN2021112499-appb-100034
    Figure PCTCN2021112499-appb-100035
    Figure PCTCN2021112499-appb-100036
    Figure PCTCN2021112499-appb-100037
    Figure PCTCN2021112499-appb-100038
  26. A pharmaceutical composition comprising the compound according to any one of claims 1-25 or the pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable excipient.
  27. A method for treating a proliferative disorder comprising administering to a subject in need thereof a therapeutically effective amount of the compound according to claims 1-25 or the pharmaceutically acceptable salt thereof, or the pharmaceutical composition according to claim 26.
  28. The method according to claim 27, wherein the proliferative disorder is selected from the group consisting of a cancer, a pancreatic cancer, a lung adenocarcinoma, a colorectal cancer, a gall bladder cancer, a thyroid cancer, NSCLC or a bile duct cancer.
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WO2023172940A1 (en) 2022-03-08 2023-09-14 Revolution Medicines, Inc. Methods for treating immune refractory lung cancer
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