WO2023008377A1 - Food/drink having improved taste and aroma - Google Patents

Food/drink having improved taste and aroma Download PDF

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Publication number
WO2023008377A1
WO2023008377A1 PCT/JP2022/028638 JP2022028638W WO2023008377A1 WO 2023008377 A1 WO2023008377 A1 WO 2023008377A1 JP 2022028638 W JP2022028638 W JP 2022028638W WO 2023008377 A1 WO2023008377 A1 WO 2023008377A1
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Prior art keywords
food
content
drink
ppm
taste
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PCT/JP2022/028638
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French (fr)
Japanese (ja)
Inventor
大輝 伊藤
万優 廣瀬
Original Assignee
株式会社Mizkan Holdings
株式会社Mizkan
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Application filed by 株式会社Mizkan Holdings, 株式会社Mizkan filed Critical 株式会社Mizkan Holdings
Priority to JP2023532685A priority Critical patent/JP7432213B2/en
Publication of WO2023008377A1 publication Critical patent/WO2023008377A1/en
Priority to JP2024010318A priority patent/JP2024028656A/en

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00Soups; Sauces; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/60Salad dressings; Mayonnaise; Ketchup
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor

Definitions

  • the present invention relates to food and drink with improved taste and aroma.
  • the taste of food and drink consists of five basic tastes: sweetness, saltiness, sourness, bitterness, and umami. It's becoming On the other hand, richness is composed of complexity, breadth, and persistence of taste, and the strength and weakness of these elements are related to the above basic tastes, especially umami.
  • umami ingredients such as sodium glutamate, sodium inosinate, and sodium guanylate are added to food and drink.
  • umami ingredients such as sodium glutamate, sodium inosinate, and sodium guanylate are added to food and drink.
  • yeast extracts and protein hydrolysates are used as raw materials containing umami components.
  • an off-taste or an off-taste may occur, which poses a problem of changing the original flavor of the food or drink.
  • Patent Document 1 discloses a umami enhancer containing a combination of methional and a specific lower fatty acid ester.
  • food and drink are required not only to enhance umami, but also to have an impact of flavor (taste and aroma) at the moment they are taken in the mouth. being considered.
  • Patent Document 2 describes that adding fumaric acid and citric acid together with ⁇ -glutamyl peptide to food and drink enhances the taste of the food and drink.
  • Patent Document 3 discloses (A) sterols, etc., (B) protein hydrolysates, (C) potassium salts, (D) spices (allspice, mace, coriander, celery seed, cardamom, cinnamon, thyme and fennel). ) in a specific ratio can maintain the impact of the initial taste and the persistence of the aftertaste even when the amount of salt used is reduced.
  • JP 2016-131560 Japanese Patent Application Laid-Open No. 2020-74702 JP 2017-70217 A
  • An object of the present invention is to provide a food or drink that has a strong impact of flavor (taste and aroma) the moment it is put in the mouth, in view of the above-mentioned circumstances.
  • the present inventors have found that by adding a certain amount of one or more of terpinyl acetate, apigenin, and luteolin to food and drink together with a taste component, flavor (taste and fragrance), and found that the impact of the fragrance is felt strongly, leading to the completion of the present invention.
  • the present invention includes the following inventions.
  • a food or drink with improved taste and aroma containing one or more selected from terpinyl acetate, apigenin, and luteolin and a taste component.
  • the food or drink according to (1), wherein the content of luteolin is 0.00001 ppm or more and 100 ppm or less.
  • (b1) The ratio of the apigenin content to the glutamic acid content in the food or drink (apigenin content (ppm)/glutamic acid content (ppm)) is 5.0 ⁇ 10 ⁇ 9 to 1 ⁇ 10 ⁇ 2
  • the ratio of the content of apigenin to the content of inosinic acid in the food or drink is 5.0 ⁇ 10 ⁇ 10 to 1.0 ⁇ 10-3 (9) any one of (1) to (6), wherein the taste component is one or two of glutamic acid and inosinic acid, and satisfies the following (c1) and (c2); Food and drink listed in .
  • a method for improving the taste and aroma of food and drink which comprises adding one or more selected from terpinyl acetate, apigenin, and luteolin to food and drink containing a taste component or raw materials thereof.
  • a step of adding a plant material containing one or more selected from terpinyl acetate, apigenin, and luteolin to an extraction solvent (ii) Extracting components from the plant material with an extraction solvent, and a step of obtaining a plant material extract containing one or more selected from luteolin (iii) a step of adjusting the pH of the plant material extract obtained in (ii) to a relatively acidic side (16)
  • delicious foods and drinks that give a strong sense of both taste and aroma the moment they are put into the mouth are provided.
  • the mechanism is unknown, the food and drink of the present invention have a strong impact by sharply feeling the taste and aroma at the moment they are put in the mouth, regardless of the temperature or form, and the original flavor of the material is good and there is no unpleasant taste. , good palatability.
  • the food and drink with improved taste and aroma of the present invention contains one or more selected from terpinyl acetate, apigenin, and luteolin, and a taste component.
  • taste refers to tastes that are received by taste receptors in organs called taste buds that are mainly present on the tongue and that can be distinct from each other, and includes five basic tastes: sweet, sour, salty, bitter and umami.
  • Aroma refers to the aroma detected by the nose when food or drink is put in the mouth or chewed.
  • flavor refers to a complex sensation composed of taste and aroma, and is linked to overall deliciousness.
  • “Taste” in the present invention mainly refers to umami, saltiness, and sweetness among the above five basic tastes, preferably umami.
  • the "taste” in the present invention includes the taste that is felt at the moment the food or drink is put in the mouth (for example, within 2 seconds), and the taste that is felt after the food or drink is put in the mouth and before swallowing or during mastication. It includes both a taste that spreads in the mouth while being held and a taste that lasts (spread of taste).
  • the "aroma” in the present invention refers to the aroma associated with the taste felt at the moment (for example, within 2 seconds) when the food or drink is put in the mouth.
  • Terpinyl acetate (systematic name: 4-methyl-1-(1-methylethyl)-3-cyclohexen-1-ol acetate, English notation: Terpinyl acetate) used in the food or drink of the present invention has the molecular formula C 12 H 20 O 2 (molecular weight: 196.29) and CAS registry number 80-26-2.
  • Apigenin (systematic name: 5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one, English notation: Apigenine) used in the food or drink of the present invention has the molecular formula C 15 H 10 O 5 (molecular weight: 270.24) and CAS registry number 520-36-5.
  • (luteolin) Luteolin (systematic name: 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-chromenone, English notation: Luteolin) used in the food or drink of the present invention has the molecular formula C 15 H 10 O 6 (molecular weight : 286.24) and has a CAS registry number of 491-70-3.
  • Terpinyl acetate, apigenin, or luteolin in the present invention is not limited as long as it can be orally ingested, and may be synthetic or derived from plant materials containing terpinyl acetate, apigenin, or luteolin. From the viewpoint of the influence on the flavor of food and drink, it is preferable to use a synthetic product or a plant material processed product, and it is more preferable to use a synthetic product or a plant material extract.
  • plant materials containing terpinyl acetate include celery, bay leaf, parsley, cardamom, and laurel
  • examples of plant materials containing apigenin and luteolin include celery and parsley. These may be used alone or in combination of two or more.
  • the plant material processed product refers to a product processed by drying, pulverizing, extracting, refining, etc. of a plant material containing terpinyl acetate, apigenin, or luteolin.
  • an extract which is a preferred embodiment of the processed product, can be prepared by adding the terpinyl acetate-containing plant material to a solvent, allowing the mixture to stand, and then filtering.
  • it can be prepared by heating a solvent to which the plant material containing terpinyl acetate, apigenin, or luteolin has been added and allowing it to stand after being heated to a specific temperature, or by maintaining it at a constant temperature while stirring.
  • the solvent used for extraction includes water, ethanol, and a mixed solvent of water-ethanol as a neutral solvent, with water being preferred.
  • the pH of the solvent used for extraction within a predetermined range, because it facilitates the extraction of terpinyl acetate, apigenin, and luteolin.
  • a plant material containing at least one of terpinyl acetate, apigenin, or luteolin is added to the extraction solvent, and the pH of the extraction solvent is adjusted to 6.5 or more and 11.0 or less at room temperature to extract the components.
  • the pH of the extraction solvent may be 6.5 or higher, 7.0 or higher, 7.5 or higher, 8.0 or higher, or 9.0 or higher. 0.5 or less, or 10.0 or less.
  • the pH of the extraction solvent may be adjusted after adding the plant material containing at least one of terpinyl acetate, apigenin, or luteolin to the extraction solvent.
  • a plant material containing at least one of terpinyl acetate, apigenin, or luteolin may be added to the solvent.
  • the extraction solvent may be added with a pH adjuster (e.g. sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, potassium gluconate, lactic acid, citric acid, tartaric acid, malic acid and acetic acid). etc.) can be dissolved, but it is particularly preferable to use carbonates such as sodium carbonate, potassium carbonate, or calcium carbonate.
  • a pH adjuster e.g. sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, potassium gluconate, lactic acid, citric acid, tartaric acid, malic acid and acetic acid.
  • carbonates such as sodium carbonate, potassium carbonate, or calcium carbonate.
  • the amount of the solvent to be used is not particularly limited. For example, it may be 10 times or more, preferably 20 times or more, the plant material (dry weight) containing terpinyl acetate, apigenin, or luteolin, but it may be concentrated after extraction. or isolation, it is preferably 100 times or less for convenience of operation.
  • the extract of terpinyl acetate, apigenin, or luteolin can be used as it is in the food or drink of the present invention, but if necessary, it may be subjected to concentration treatment, hot air drying, steam drying, freeze drying, drying treatment such as spray drying, separation and purification. It may be subjected to treatment, decolorization treatment, etc., and used as a concentrate, dried product, or the like.
  • the content (ppm) of terpinyl acetate in the food or drink of the present invention depends on the type of the food or drink, but is 0.00000001 ppm or more, preferably 0.000001 ppm or more, more preferably 0.0001 ppm or more, and It is 10 ppm or less, preferably 1 ppm or less, more preferably 0.01 ppm or less. If the content (ppm) of terpinyl acetate is less than 0.00000001 ppm, the impact of the flavor and the spread of the taste will not be achieved, and the taste as a whole will be lost.
  • flavor impact means that a strong sense of improvement in taste and aroma is felt the moment the food is put in the mouth.
  • the terpinyl acetate contained in the food and drink of the present invention may be contained in food materials such as edible plants that are raw materials of the food and drink, and are added separately from the food and food when the food and drink of the present invention are produced. However, it may be generated along with the production of the food or drink of the present invention. Alternatively, terpinyl acetate derived from two or more of these may be combined to satisfy the above predetermined content and/or ratio.
  • terpinyl acetate When terpinyl acetate is added externally during the production of the food or drink of the present invention, a highly pure terpinyl acetate reagent that has been purified and extracted may be added, or in the form of some processed plant material (e.g., extract) containing terpinyl acetate. may be added at However, the majority (more preferably all) of terpinyl acetate contained in the food or drink is preferably derived from some kind of foodstuff, more preferably derived from edible plants.
  • the content (ppm) of apigenin in the food or drink of the present invention depends on the type of food or drink, but is 0.00001 ppm or more, preferably 0.0001 ppm or more, more preferably 0.001 ppm or more, and 10 ppm. Below, preferably 1 ppm or less, more preferably 0.1 ppm or less. If the content (ppm) of apigenin is less than 0.00001 ppm, the impact of the flavor and the spread of the taste will not be obtained, and the overall deliciousness will be lost. If the apigenin content (ppm) is more than 10 ppm, the impact of the flavor and the spread of the taste are not obtained, and the overall taste is not good.
  • the apigenin contained in the food and drink of the present invention may be contained in food materials such as edible plants that are raw materials of the food and drink, or may be added separately from the food and food when the food and drink of the present invention are produced. It may occur along with the production of the food or drink of the present invention. Alternatively, the predetermined content and/or ratio may be satisfied as a result of combining apigenin derived from two or more of these.
  • a high-purity apigenin reagent that has been purified and extracted may be added. may However, the majority (more preferably all) of the apigenin contained in the food or drink is preferably derived from some kind of foodstuff, and more preferably derived from edible plants.
  • the content (ppm) of luteolin in the food and drink of the present invention is 0.00001 ppm or more, preferably 0.0001 ppm or more, more preferably 0.001 ppm or more, and is 100 ppm, depending on the type of food and drink. Below, preferably 1 ppm or less, more preferably 0.1 ppm or less. If the content (ppm) of luteolin is less than 0.00001 ppm, there is no flavor impact and no spread of taste, and there is no overall deliciousness. If the content (ppm) of luteolin is more than 100 ppm, the flavor impact and the spread of the taste are not obtained, and the overall deliciousness is lost.
  • the luteolin contained in the food and drink of the present invention may be contained in food materials such as edible plants that are raw materials of the food and drink, or may be added separately from the food and food when the food and drink of the present invention are produced. Well, it may be generated along with the production of the food or drink of the present invention. Alternatively, the predetermined content and/or ratio may be satisfied as a result of combining luteolin derived from two or more of these sources.
  • a high-purity luteolin reagent that has been purified and extracted may be added. may However, the majority (more preferably all) of the luteolin contained in the food or drink is preferably derived from some kind of foodstuff, more preferably derived from edible plants.
  • the ratio of the apigenin content to the terpinyl acetate content is, for example, 0.01 ⁇ 100,000, 0.1-100,000, 0.1-10,000, 1-10,000, 1-5,000, or 1-1,000.
  • the ratio of the luteolin content to the terpinyl acetate content is, for example, 0.0001 ⁇ 100,000, 0.001 to 100,000, 0.001 to 10,000, 0.01 to 10,000, 0.01 to 5,000, or 0.01 to 1,000.
  • the ratio of the content of luteolin to the content of apigenin is, for example, 0.0001 to 100, 0.001-100, 0.001-10, 0.005-10, 0.005-5, 0.01-1.
  • the ratio of the total content of apigenin and luteolin to the content of terpinyl acetate is, for example, 0.01 to 100,000, 0.1 to 100,000, 0.1 to 10,000, 1 to 10,000, 1 to 5,000, 1 to 2,000.
  • the "taste component" used in the food or drink of the present invention includes umami components such as amino acids and nucleic acids, sweet components such as sugars, sour components such as organic acids, and salty components such as common salt and other salts.
  • the umami component is preferred.
  • the umami component may be an amino acid-based, nucleic acid-based, or organic acid-based umami component.
  • amino acid-based umami components include glutamic acid, aspartic acid, oxyglutamic acid, lysine, histidine, taurine, betaine, glycine, creatine, alanine, arginine, and salts thereof
  • nucleic acid-based umami components include inosinic acid.
  • guanylic acid, or salts thereof, and organic acid-based umami components include succinic acid, acetic acid, citric acid, malic acid, or salts thereof.
  • the umami component may be a food material containing each of the above components or a processed product thereof.
  • foods containing umami components or processed products thereof include powders and extracts of vegetables (e.g., carrots, onions, celery, Chinese cabbage, cabbage, etc.), powders of seaweeds (e.g., kelp, wakame, etc.) and Their extracts, mushrooms (e.g., shiitake, shimeji, etc.) powders and their extracts, seafood (e.g., bonito, mackerel, horse mackerel, sardines, shrimp, scallops, oysters, etc.) powders and their extracts, meat ( Examples include beef, pork, chicken, etc.) powders and extracts thereof.
  • vegetables e.g., carrots, onions, celery, Chinese cabbage, cabbage, etc.
  • powders of seaweeds e.g., kelp, wakame, etc.
  • mushrooms e.g., shiitake, shimeji, etc.
  • seafood e.g., bonito, mackerel, horse macker
  • the ratio of the content of terpinyl acetate to the content of glutamic acid (terpinyl acetate content (ppm)/glutamic acid content (ppm)) in the food and drink of the present invention is sufficient for flavor impact and spread of taste. From the viewpoint of effects, etc., it is usually 5.0 ⁇ 10 ⁇ 12 to 1 ⁇ 10 ⁇ 2 , preferably 5.0 ⁇ 10 ⁇ 10 to 6.0 ⁇ 10 ⁇ 3 , more preferably 5.0 ⁇ 10 ⁇ 3 . 9 to 6.0 ⁇ 10 ⁇ 4 .
  • the content of glutamic acid in the food and drink of the present invention is not particularly limited as long as the effect of the present invention can be exhibited, but it is 500 ppm or more, preferably 750 ppm or more, more preferably 1000 ppm or more, and the upper limit is usually 20000 ppm or less. It is preferably 15000 ppm or less, more preferably 12000 ppm or less, still more preferably 5000 ppm or less, or 4000 ppm or less, or 3000 ppm or less.
  • the ratio of the terpinyl acetate content to the inosinic acid content (terpinyl acetate content (ppm)/inosinic acid content (ppm)) in the food or drink of the present invention determines the impact of flavor and spread of taste. From the viewpoint of sufficient effect, etc., it is usually 6.0 ⁇ 10 ⁇ 11 to 0.2, preferably 6.0 ⁇ 10 ⁇ 9 to 0.1, more preferably 6.0 ⁇ 10 ⁇ 8 to 0.01.
  • the content of inosinic acid in the food and drink of the present invention is not particularly limited as long as the effect of the present invention can be exhibited, but is 10 ppm or more, preferably 15 ppm or more, more preferably 100 ppm or more, and the upper limit is usually 2000 ppm or less. , preferably 1500 ppm or less, more preferably 1200 ppm or less, still more preferably 500 ppm or less, or 400 ppm or less, or 300 ppm or less.
  • the ratio of the content of apigenin to the content of glutamic acid (apigenin content (ppm)/glutamic acid content (ppm)) in the food or drink of the present invention is sufficient for the impact of flavor and the spread of taste. From the point of view, it is usually 5.0 ⁇ 10 ⁇ 9 to 1.0 ⁇ 10 ⁇ 2 , preferably 4.0 ⁇ 10 ⁇ 8 to 1.0 ⁇ 10 ⁇ 3 .
  • the ratio of the content of apigenin to the content of inosinic acid (apigenin content (ppm)/inosinic acid content (ppm)) in the food or drink of the present invention is sufficient for flavor impact and spread of taste. From the viewpoint of effect, etc., it is usually 5.0 ⁇ 10 ⁇ 10 to 1.0 ⁇ 10 ⁇ 3 , preferably 5.0 ⁇ 10 ⁇ 8 to 1.0 ⁇ 10 ⁇ 4 .
  • the ratio of the content of luteolin to the content of glutamic acid (luteolin content (ppm)/glutamic acid content (ppm)) in the food and drink of the present invention is sufficient for the impact of flavor and the spread of taste. From the point of view, etc., it is usually 5.0 ⁇ 10 ⁇ 9 to 1.0 ⁇ 10 ⁇ 1 , preferably 5.0 ⁇ 10 ⁇ 7 to 1.0 ⁇ 10 ⁇ 2 .
  • the ratio of the content of luteolin to the content of inosinic acid (luteolin content (ppm)/inosinic acid content (ppm)) in the food and drink of the present invention is sufficient for flavor impact and spread of taste. From the viewpoint of effect, etc., it is usually 5.0 ⁇ 10 ⁇ 10 to 1.0 ⁇ 10 ⁇ 2 , preferably 5.0 ⁇ 10 ⁇ 8 to 1.0 ⁇ 10 ⁇ 3 .
  • the concentration of each component of terpinyl acetate, apigenin, luteolin, glutamic acid, and inosinic acid in the food and drink of the present invention can be determined when the amount of the component to be added is clear (for example, when the food and drink are mixed with each refined component). can be calculated from the blending amount and the volume of the food or drink, and if the blending amount of the component is unknown, follow or follow the method described in the reference examples below. can be calculated.
  • ppm in this specification means mass concentration (w/w).
  • food and drink refers to food and drink that can be eaten as it is, and compositions for preparing food and drink that are used to prepare food and drink that are used as they are. composition for).
  • Food and drink that can be eaten as is includes food and drink that are eaten without adding any ingredients, as well as food and drink that are eaten by adding seasonings as necessary by the person who eats them. is also included.
  • Food and drink that can be eaten as it is is not particularly limited as long as it is a food and drink that requires improvement in taste and aroma. ), breads, pizzas, cereals, lunch boxes, vegetable protein-containing foods, and the like.
  • Beverages include, for example, fruit juice-containing beverages [for example, citrus fruits (mandarin orange, orange, lemon, lime, grapefruit, yuzu, kabosu, sudachi, bergamot, pink grapefruit, hassaku, calamansi, etc.), tropical fruits (pineapple, banana, guava, mango, acerola, papaya, passion fruit, etc.), lychee, strawberry, apple, peach, grape, white grape, cassis, raspberry, pomegranate, plum, pear, apricot, plum, kiwifruit, melon, blueberry, fruit juice such as acai, lemonade Etc., beauty drinks, smoothies, etc.]; Dairy-containing beverages (for example, milk and its processed products such as skim milk powder and whole milk powder, concentrated milk, yogurt, fresh cream, condensed milk, butter, skim milk, Cream powder, sweetened milk powder, modified milk powder, whey powder, buttermilk powder, and other dairy-
  • juices such as tomatoes, carrots, and pumpkins; drinks, near water, fruit-flavored drinks), carbonated drinks; jelly drinks; , jasmine tea, rosehip tea, chamomile tea, roasted tea, blended tea (grains such as adlay, barley, brown rice, soybeans, corn, persimmon leaves, loquat leaves, bamboo grass, Gynostemma pentaphyllum, Angelica keiskei, Houttuynia cordata leaves, etc. , tea blended with kelp, safflower, shiitake mushrooms, litchi, etc.)]; coffee beverages; powdered beverages (e.g.
  • Distilled liquor such as liquor, shochu, whiskey, brandy, and spirits, mixed liquor such as liqueur, which is a mixture of distilled liquor with auxiliary ingredients such as sugars, cocktails, fizz, and chuhai that are made by adding fruit juice, flavor, carbon dioxide gas, etc. to these liquors etc.).
  • Confectioneries include, for example, jelly, pudding, chocolate, bars (snack bars), frozen desserts (ice cream, sherbet, etc.), and among these, jelly and frozen desserts (ice cream, sherbet, etc.) are preferred.
  • cooked rice examples include white rice, salted rice, red rice, steamed rice, mixed rice, mixed rice, rice balls, sushi rice, mochi, and dumplings.
  • rice includes glutinous rice, glutinous rice, non-washed rice with different degrees of rice polishing, brown rice, and the like.
  • Cooked products of these rice products and other ingredients may also be used, such as sushi, chirashizushi, curry rice, rice bowls, fried rice, tenshinhan, and the like.
  • Noodles are made mainly from cereal flour such as wheat flour, rice flour, buckwheat flour, and beans, and are shaped into noodles, plates, ribbons, etc., and then boiled, stewed, or steamed. food that is cooked in Examples of noodles include soba, udon, kishimen, ramen, Chinese noodles, pasta, macaroni, somen, pho, Korean cold noodles, vermicelli, and the like.
  • Prepared food refers to foods obtained by cooking ingredients such as meat, seafood, eggs, milk, vegetables, fruits, herbs, and seaweed using appropriate methods.
  • Side dishes include, for example, pickles, boiled foods, grilled foods, fried foods, stir-fried foods, steamed foods, and seasoned foods.
  • Soup refers to food containing a lot of water, obtained by cooking ingredients such as meat, seafood, eggs, milk, vegetables, fruits, herbs, and seaweed in an appropriate manner.
  • soups include miso soup, soup, potage, consommé soup, curry soup, wakame seaweed soup, and egg soup.
  • Bread consists mainly of wheat flour or wheat flour added with yeast, or water, salt, fruits such as grapes, vegetables, eggs and their processed products, sugars, It is a food with a moisture content of 10% or more, which is made by kneading and fermenting edible oil, milk, dairy products, etc. (hereinafter referred to as "bread dough").
  • Pizza is a food that is made by kneading flour, water, salt, yeast, sugar, and a small amount of olive oil, then fermenting the dough, rolling it out thinly, putting ingredients on it, and baking it in an oven or a special kiln. be.
  • Vegetable protein-containing foods refer to foods and drinks made from raw materials rich in vegetable protein, and processed beans are particularly suitable.
  • Raw materials include whole grains such as soybeans, peas, mung beans, chickpeas, peanuts, lupines, pigeon peas, nata beans, vine beans, kidney beans, adzuki beans, cowpeas, lentils, fava beans, and locust beans.
  • Its pulverized product can be mentioned, and lees obtained by industrially extracting oils and fats and starch from these can also be used.
  • specific vegetable protein-containing foods of the present invention include soybean meat, natto (fermented soybeans), and the like.
  • the protein content contained in the vegetable protein-containing food is not particularly limited, but may be 3% by mass or more and 50% by mass or less. More specifically, the lower limit is 3 mass% or more, 4 mass% or more, 5 mass% or more, 6 mass% or more, 7 mass% or more, 8 mass% or more, 9 mass% or more, 10 mass% or more Well, the upper limit may be 50% by mass or less, 45% by mass or less, 40% by mass or less, 35% by mass or less, or 30% by mass or less.
  • composition for preparing food and drink to be eaten is not particularly limited, but for example, seasonings, retort foods, compositions for preparing beverages, and compositions for preparing soups. , topping materials, and the like. Among these, seasonings and retort foods are preferred, and seasonings are more preferred.
  • Seasonings include, for example, soups (noodle soup, nabetsuyu, etc.), sauces (sesame-containing seasonings such as sesame sauce, yakiniku sauce, etc.), soup stocks (for example, vegetable stocks, seafood stocks, meat stocks, etc.) Dashi), extracts (e.g. vegetable extracts, seafood extracts, meat extracts), sauces (pasta sauce, pizza sauce, Worcestershire sauce, ketchup sauce, oyster sauce, salsa sauce, sambal sauce, chili sauce, demi-glace sauce, white sauce) (bechamel sauce), etc.), dressings (non-oil dressing, separated dressing, emulsified dressing, etc.), vinegar, seasoning vinegar (e.g.
  • seasoning vinegar seasoning vinegar for vinegared dishes, seasoning vinegar for sushi rice, seasoning for pickles (e.g. pickles, etc.) liquid, sweet vinegar, etc.
  • seasonings for cooked rice soy sauce (used for dipping soy sauce, soy sauce for pouring, and cooking purposes described in Japanese Agricultural Standards (Ministry of Agriculture, Forestry and Fisheries Notification No. 1703, September 13, 2004) or seasonings similar to soy sauce used for the above purposes), ponzu seasonings (ponzu, ponzu soy sauce), seasonings for natto, seasonings for pickles, seasonings for meat, seasonings containing spices , chutney, mustard, mayonnaise and the like.
  • soups, sauces, soups, extracts, dressings, sauces, soy sauce, and ponzu seasonings are preferred.
  • Retort food refers to food that is packed with cooked or semi-cooked food in a retort pouch or can, and that is retort-heated (most commonly, 120°C, pressurized heating for 30-60 minutes).
  • the cooked or semi-cooked food includes, for example, the above-described food itself, or a food that can be obtained by simple cooking (for example, by adding ingredients and cooking with heat). be done.
  • compositions for preparing beverages include, for example, concentrated types of beverages. This is diluted with water or a suitable beverage (eg, the beverages exemplified above) prior to consumption.
  • a recommended dilution ratio is, for example, 1.1 to 50 times, preferably 2 to 20 times, more preferably 3 to 12 times, still more preferably 4 to 8 times.
  • a composition for preparing a soup is a soup that is heated with vegetables, meat, seafood, dairy products, rice, or noodles, or that is prepared into a food such as soup by combining the above ingredients after cooking. Elements are mentioned.
  • the soup base may be in a form (straight type) that can be used as it is, a concentrated type that is used after being mixed (diluted) with cold water, hot water, broth or other liquids, or a powder type.
  • topping materials include rice topping materials, noodle topping materials, bread topping materials, and salad topping materials.
  • topping materials for cooked rice foods include furikake, rice ball mix, rice porridge mix, ochazuke mix, rice porridge mix, mixed rice mix, and cooked rice mix.
  • the food and drink to be the subject of the present invention include, in addition to the above general food and drink, foods other than pharmaceuticals that can be ingested for the purpose of maintaining and improving health, such as health foods, functional foods, and foods with health claims. , or is used in the sense of including food for special uses.
  • Health foods include foods provided under the names of nutritional supplements, health supplements, supplements, and the like.
  • Foods with health claims are defined by the Food Sanitation Law or the Health Promotion Law. This includes foods with function claims that can display the content notified to the Commissioner of the Consumer Affairs Agency regarding the functionality of the food.
  • Foods for special dietary uses include foods for the sick, foods for the elderly, foods for infants, foods for pregnant women, etc., which are labeled as being suitable for specific subjects or patients with specific diseases.
  • indications such as specific health effects and functions of nutritional ingredients attached to food and drink are indicated on product containers, packaging, instructions, and attached documents, product flyers and pamphlets, newspapers and magazines, etc. can be advertisements for products, etc.
  • the form of food and drink may be any form suitable for eating, such as solid, liquid, granule, grain, powder, capsule, cream, or paste.
  • the shape of the above-mentioned health food and the like includes liquid (including syrup, milk, and suspension), tablet, round, capsule, powder, granule, fine grain, and lozenge. etc. are preferred.
  • the food and drink of the present invention can contain other raw materials and additives depending on the type of food and drink.
  • Other raw materials and additives include oils and fats, alcoholic beverages, sweeteners, acidulants, spices or spice extracts, viscosity modifiers, pigments, stabilizers, antioxidants, pH adjusters and the like.
  • the combination and content of these other raw materials and additives are not particularly limited, and can be appropriately set according to the type of food or drink.
  • one or more selected from terpinyl acetate, apigenin, and luteolin are added to food and beverages containing taste components or raw materials thereof.
  • a method for improving the taste and aroma of food and drink characterized by the above is provided.
  • the timing of adding one or more selected from terpinyl acetate, apigenin, and luteolin to a food or drink containing taste components or its ingredients is not particularly limited.
  • the timing includes, for example, during production of the food and drink, after production of the food and drink, and before eating.
  • a preferred embodiment of the method for producing food or drink of the present invention comprises preparing a plant material extract by a method including the following steps (i) to (iii). (i) A step of adding a plant material containing one or more selected from terpinyl acetate, apigenin, and luteolin to an extraction solvent (ii) Extracting components from the plant material with an extraction solvent, and a step of obtaining a plant material extract containing one or more selected from luteolin (iii) a step of adjusting the pH of the plant material extract obtained in (ii) to be relatively acidic.
  • Another preferred embodiment of the method for producing a food or drink of the present invention further comprises a step of adjusting the pH of the plant material extract to 6.5 or more and 12.0 or less after the step (ii).
  • step (i) a plant material containing one or more selected from terpinyl acetate, apigenin, and luteolin is added to the extraction solvent.
  • the types of plant material and extraction solvent are as described above.
  • step (ii) components are extracted from the plant material with an extraction solvent to obtain a plant material extract containing one or more selected from terpinyl acetate, apigenin, and luteolin.
  • the pH of the extraction solvent is 6.5 or higher, 7.0 or higher, 7.5 or higher, 8.0 or higher, or 9.0 or higher. 10.5 or less, 10.0 or less.
  • the pH of the plant material extract is adjusted to neutral to alkaline at room temperature, for example , 6.5 or more and 12.0 or less. More specifically, the pH of the plant material extract is 6.5 or higher, 7.0 or higher, 7.5 or higher, 8.0 or higher, or 9.0 or higher. Below, it may be 11.5 or less and 11.0 or less.
  • the pH of the solution containing these components may be adjusted. good.
  • the pH is adjusted by adding a pH adjusting agent (e.g., sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, potassium gluconate, lactic acid, citric acid, tartaric acid, malic acid, acetic acid, etc.).
  • a pH adjusting agent e.g., sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, potassium gluconate, lactic acid, citric acid, tartaric acid, malic acid, acetic acid, etc.
  • carbonates such as sodium carbonate, potassium carbonate, or calcium carbonate.
  • step (iii) the pH of the plant material extract obtained by the above extraction is adjusted to be relatively acidic.
  • the technical significance of this step is that the pH at room temperature of the plant material extract obtained after the extraction step of step (ii) (sometimes simply referred to as “step (ii) extract”) is is also adjusted to be relatively acidic to immobilize the ingredients (terpinyl acetate, apigenin, or luteolin) of the food and drink of the present invention.
  • the difference in pH drop between the pH of the step (ii) extract i.e. the extract obtained after the extraction step of step (ii)
  • the pH of the extraction solvent used in step (i) is 0.1. It is preferable to adjust the pH to 10.0 or less.
  • the lower limit is 0.1 or more, 0.2 or more, 0.3 or more, 0.4 or more, 0.5 or more, 0.6 or more and 0.7 or more, 0.8 or more, 0.8 or more. 9 or more and 1.0 or more, and the upper limits are 10.0 or less, 9.0 or less, 8.0 or less, 7.0 or less, 6.0 or less, 5.0 or less, 4.0 or less3. 0 or less is acceptable.
  • the solvent used for extraction may optionally be heated.
  • the heating conditions are not particularly limited, but the heating temperature can be in the range of, for example, 50° C. or more and 105° C. or less, and the treatment time can be in the range of, for example, 30 seconds or more and less than 30 minutes. More specifically, the lower limit of the heating temperature is, for example, 50°C or higher, or 55°C or higher, or 60°C or higher, or 65°C or higher, or 70°C or higher, or 75°C or higher, or 80°C or higher, or 85°C or higher. , or 90 ° C. or higher, or 95 ° C. or higher, the upper limit is usually 105 ° C. or lower, or 100 ° C.
  • the lower limit of the heating time is, for example, 30 seconds or more, or 1 minute or more, or 2 minutes or more
  • the upper limit can be, for example, less than 30 minutes, less than 25 minutes, less than 20 minutes, less than 15 minutes, or less than 10 minutes, or less than 5 minutes.
  • the heating temperature and heating time are almost interdependent, and while the higher the heating temperature, the shorter the heating time, the longer the heating time, the lower the heating temperature. be. Therefore, the relationship between the heating temperature and the heating time should be taken into account, and the respective ranges should be set appropriately. Specifically, heat extraction may be performed in the range of 70 ° C. or higher and 105 ° C.
  • heat extraction may be performed in the range of 80 ° C. or higher and 105 ° C. or lower for less than 20 minutes. You may heat extraction for less than 15 minutes in the range below °C.
  • the pH of the extract after being adjusted to be relatively acidic in step (iii) may be adjusted within a predetermined range.
  • the pH of the extract (plant material extract) adjusted to be relatively acidic in step (iii) can be adjusted to be 1.5 or more and less than 7.0 at room temperature.
  • the lower limit may be 1.5 or more, 2.0 or more, 2.5 or more, or 3.0 or more
  • the upper limit is not particularly limited, but usually less than 7.0 and 6.5. less than, less than 6.0, less than 5.5, less than 5.0, less than 4.6, less than 4.0.
  • Samples for quantification were prepared by the following procedure. Sample: Luteolin and apigenin reagents with known concentrations were diluted and tested for LCMS as standards with component concentrations of 0.00025 ppm, 0.0005 ppm, 0.005 ppm, 0.05 ppm and 0.5 ppm.
  • a sample to be measured (for example, 2% parsley extract) was diluted with 4 volumes of methanol, filtered, and then subjected to LCMS.
  • MS conditions ion source temperature: 200°C
  • Ionization method ESI (ionization voltage 5.0 eV)
  • MS scan m/z 50-1,500
  • Each sample was analyzed for H+ adduct ions in positive mode, and the The peak area of the peak was determined by identifying the peak of the component inside. From the obtained peak area of each component, the concentration of each component contained in each sample was calculated in consideration of the dilution ratio with the solvent.
  • Test Example 1 Examination of terpinyl acetate content (evaluation by soup) (1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare terpinyl acetate solutions having the concentrations shown in Table 1 for test products 1 to 7.
  • Dried bonito stock, dark soy sauce, sugar, mirin, salt, terpinyl acetate solution, and water were mixed according to the blending amounts (mass%) shown in Table 1 to prepare test products 1 to 9.
  • the control was a soup containing 66.5% by mass of water without adding the terpinyl acetate solution, with the same blending amounts of dried bonito stock, dark soy sauce, sugar, mirin, and salt.
  • the terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
  • Identification test (Analytical sensory evaluation expert panelist) The analysis-type sensory evaluation expert panelists are expert panelists whose ability to judge tastes and aromas is guaranteed by the following identification test (I) and identification test (II).
  • Identification test (I): Taste identification test Five tastes (sweetness: sugar taste, sourness: tartaric acid taste, umami: sodium glutamate taste, salty taste: sodium chloride taste, bitterness: caffeine taste) A taste discrimination test in which each taste sample is accurately identified from a total of 7 samples in which 2 distilled water is added to an aqueous solution with a concentration close to the threshold value.
  • Discrimination test (II): Concentration difference discrimination test for accurately discriminating the difference in concentration between five types of saline solutions and acetic acid solutions having slightly different concentrations.
  • the sensory evaluation was performed in the order of (i) sample presentation, (ii) adjustment of sensory evaluation items, (iii) trial evaluation/calibration, and (iv) final evaluation.
  • (i) Presentation of samples Sample presentation was set as follows in order to eliminate the panelist's bias in the sensory evaluation and improve the accuracy of the evaluation.
  • a sample filled and sealed in a 100 mL PET bottle was brought to room temperature (25° C.), and for each evaluation, about 1 teaspoon (about 5 mL) was transferred from the container to a 20 mL plastic cup with a measuring spoon and presented to each panelist. At that time, the samples from each test plot were presented at random without informing the panelists of the test plot numbers and contents of the samples.
  • evaluation criteria The sensory evaluation was performed by four panelists specializing in analytical sensory evaluation according to the following evaluation criteria.
  • the evaluation items were "flavor impact”, “flavor spread”, and “comprehensive evaluation (overall deliciousness)" of the soup.
  • the control was evaluated as 3 points, and each evaluation item was evaluated in the following 5 stages. Calculation of evaluation points was a weighted average of evaluations by four persons. A 5-point evaluation of 3.5 points or more is acceptable, 4 points or more indicates a good effect, 4.5 points or more indicates a better effect, and 5 points indicates the best effect. bottom. In addition, when the evaluation score for each evaluation item was less than 3 even for one item, the evaluation score was disqualified.
  • Test Example 2 Examination of terpinyl acetate content (evaluation by dressing) (1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare terpinyl acetate solutions having the concentrations shown in Table 2, Test Products 10 to 15.
  • the terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
  • Test Example 3 Effect of glutamic acid content (evaluation by soup) (1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
  • test products 16 to 20 Dried bonito stock, dark soy sauce, sugar, mirin, salt, sodium glutamate, terpinyl acetate solution, and water were mixed according to the blending amounts (mass%) shown in Table 3 to prepare test products 16 to 20.
  • a control was prepared by adding no terpinyl acetate solution to each of the test products 16 to 20.
  • the terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
  • Test Example 4 Examination of inosinic acid content (evaluation by soup) (1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
  • test products 21 to 26 Dried bonito stock, dark soy sauce, sugar, mirin, salt, sodium inosinate, terpinyl acetate solution, and water were mixed according to the blending amounts (mass%) shown in Table 4 to prepare test products 21 to 26.
  • a control was prepared by adding no terpinyl acetate solution to each of the test products 21 to 26.
  • the terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
  • Test Example 5-1 Evaluation with Tomato Sauce
  • Test Product Terpinyl acetate manufactured by Inoue Koryo Co., Ltd., Specification: 100%
  • This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
  • the terpinyl acetate content, glutamic acid content, and inosinic acid content in the prepared test product were measured according to Reference Examples.
  • Test Example 5-2 Evaluation by Soup for Yosenabe (1) Preparation of Test Product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
  • the terpinyl acetate content, glutamic acid content, and inosinic acid content in the prepared test product were measured according to Reference Examples.
  • liquid seasonings (tomato sauce, yosenabe sauce), which are preferred embodiments of foods and drinks, by adding a predetermined amount of terpinyl acetate to the taste component, Flavor impact, spread of taste, and effect of improving overall deliciousness were recognized (test products 27 and 28).
  • the tomato sauce the tomato-derived umami and aroma were enhanced, and the balance with the sourness was obtained, resulting in a coherent flavor.
  • the dipping sauce for yosenabe had a sharp soy sauce flavor, and a good soy sauce flavor was obtained that was fresh and had little miscellaneous taste.
  • Test Example 6-1 Evaluation with pasta (1) Preparation of test product
  • the tomato sauce (test product 27) prepared in Test Example 5-1 and its control were heated to 60°C. After boiling 100 parts by weight of dry pasta, 150 parts by weight of the tomato sauce of the above test product 27 was mixed with the pasta to prepare tomato-flavored spaghetti as test product 29. Also, tomato-flavored spaghetti prepared in the same manner as the control for test product 27 was used as a control.
  • Test Example 6-2 Evaluation with lettuce salad (1) Preparation of test product Tear two pieces of lettuce by hand into about 3 cm squares, cut mini tomatoes in half and mix, then prepare the non-oil dressing prepared in Test Example 2 ( Test Item 12) Test Item 30 was lettuce salad with 1 ⁇ 2 tsp. A lettuce salad prepared in the same manner as the control for Test Product 12 was also used as a control.
  • Test Examples 6-3, 6-4 Evaluation by Udon and Somen
  • Test product 31 was kake udon prepared by boiling one ball (180 g) of commercially available udon noodles and then adding 50 parts by weight of the broth of test product 4 above and mixing them. Kake-udon prepared in the same manner as the control for test product 4 was used as a control.
  • test product 4 prepared in Test Example 1 and the control were each cooled to 10°C in a refrigerator.
  • Test product 32 was chilled somen noodles prepared by boiling commercially available somen noodles, exposing them to cold water, and pouring 25 mL of the cooled soup of test product 4 above.
  • somen noodles prepared in the same manner as the control for test product 4 were used as a control.
  • Test Example 6-5) Evaluation by mixed pot (1) Preparation of test product
  • the soup for mixed pot (test product 28) prepared in Test Example 5-2 and the control were each heated to 95°C. 100 g of chicken thigh meat cut into 3 cm squares, 1/8 Chinese cabbage, 1 long onion, 2 shiitake mushrooms, 2 bundles of mizuna, and 150 g of tofu are added to the dipping sauce for the yosenabe of test product 28, and the ingredients are heated with a lid.
  • Test product 33 was a hot pot stewed until it was cooked through.
  • a casserole prepared in the same manner as the control for test product 28 was used as a control.
  • Table 6 shows the sensory evaluation results of Test Examples 6-1 to 6-5.
  • Test Example 7-1 Evaluation using soybean meat (1) Preparation of test product Dried parsley (manufactured by SB Co., Ltd.) was added to hot water at 65 ° C. so that it would be 2% by mass, and left to stand at 65 ° C. for 15 minutes. and filter paper (No. 2), and left to cool to room temperature to obtain an extract. Further, the obtained extract was adjusted to pH 10 with 20% sodium carbonate to obtain an alkaline extract (terpinyl acetate content: 46 ppb). After boiling 33 g of commercially available soybean meat in boiling water for 5 minutes, the water was squeezed to 100 g.
  • soybean meat After boiling 33g of commercially available soybean meat in boiling water for 5 minutes, the water was squeezed out to 100g. Boiled soy meat, soy sauce, sugar, sake, terpinyl acetate solution, or parsley extract are mixed according to the blending amounts (% by mass) shown in Table 7-1 below to prepare seasoned soy meat of test products 34, 35, and 36. bottom. Also, soybean meat to which 1% by mass of water was added without adding terpinyl acetate solution or parsley extract was used as a control.
  • Test Example 7-2 Evaluation by Frozen Green Onion Toro (1) Preparation of Test Item
  • the same parsley extract as in Test Example 7-1 was used. According to the blending amount (mass%) shown in Table 7-2 below, mix the thawed frozen Negitoro, soy sauce, and parsley extract, adjust the seasoned Negitoro of Test Product 37, and then overnight without touching the air. placed. Also, a seasoned green onion toro to which an equal amount of water was added without adding the parsley extract was used as a control.
  • Test Example 8 Examination of terpinyl acetate, apigenin, and luteolin content (evaluation by soup) (1) Preparation of test product Apigenin (Xi'an Natural Field Bio-Technique Co., Ltd., Specification: 98%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare an apigenin solution having the concentration shown in Table 8-1.

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Abstract

The present invention addresses the problem of providing food/drink that produces a strong sensation of taste and aroma at the instant when the food/drink is inserted into the mouth, the sensation lasting thereafter. The present invention provides food/drink having improved taste and aroma, and containing a taste component and one or more components selected from terpinyl acetate, apigenin, and luteolin.

Description

呈味と香りが向上した飲食品Food and drink with improved taste and aroma
 本発明は、呈味と香りが向上した飲食品に関する。 The present invention relates to food and drink with improved taste and aroma.
 飲食品の呈味は、甘味、塩味、酸味、苦味、及びうま味の5つの基本味で構成されており、これらの基本味の組み合わせと強弱は、飲食品の美味しさを決定する重要な要素となっている。一方、コクは、味の複雑さ、広がり、持続性で構成されており、これらの要素の強弱は、上記の基本味、特にうま味と関連性がある。 The taste of food and drink consists of five basic tastes: sweetness, saltiness, sourness, bitterness, and umami. It's becoming On the other hand, richness is composed of complexity, breadth, and persistence of taste, and the strength and weakness of these elements are related to the above basic tastes, especially umami.
 基本味のなかでもうま味がないと、味にメリハリがなく、物足りなく感じるため、グルタミン酸ナトリウム、イノシン酸ナトリウム、グアニル酸ナトリウムなどのうま味成分を飲食品に添加することが行われている。例えば、うま味成分を含む原料として、酵母エキスやたん白加水分解物が使用されている。しかしながら、いずれも原料由来の独特の風味があるため、使用量によっては異味や雑味が生じることがあり、飲食品本来の風味を変化させるという問題がある。 If there is no umami among the basic tastes, the taste lacks sharpness and feels unsatisfactory, so umami ingredients such as sodium glutamate, sodium inosinate, and sodium guanylate are added to food and drink. For example, yeast extracts and protein hydrolysates are used as raw materials containing umami components. However, since each of them has a unique flavor derived from the raw material, depending on the amount used, an off-taste or an off-taste may occur, which poses a problem of changing the original flavor of the food or drink.
 一方、飲食品のうま味を増強させる別のアプローチとして、特許文献1には、メチオナールと、特定の低級脂肪酸エステルの組み合わせを含む旨味増強剤が開示されている。しかしながら、飲食品には、うま味の増強だけなく、口に含んだ瞬間の風味(呈味と香り)のインパクトが求められており、従来より、飲食品の先味における呈味を増強させる方法が検討されている。例えば、特許文献2には、γ-グルタミルペプチドとともにフマル酸及びクエン酸を飲食品に添加することによって、飲食品の先味が増強されることが記載されている。また、特許文献3には、(A)ステロール等、(B)タンパク質加水分解物、(C)カリウム塩、(D)スパイス(オールスパイス、メース、コリアンダー、セロリシード、カルダモン、シナモン、タイム及びフェンネル)を特定の割合で含む組成物が、食塩の使用量を減じても、先味のインパクトと後味の持続性を維持できることが記載されている。 On the other hand, as another approach to enhancing the umami of food and drink, Patent Document 1 discloses a umami enhancer containing a combination of methional and a specific lower fatty acid ester. However, food and drink are required not only to enhance umami, but also to have an impact of flavor (taste and aroma) at the moment they are taken in the mouth. being considered. For example, Patent Document 2 describes that adding fumaric acid and citric acid together with γ-glutamyl peptide to food and drink enhances the taste of the food and drink. In addition, Patent Document 3 discloses (A) sterols, etc., (B) protein hydrolysates, (C) potassium salts, (D) spices (allspice, mace, coriander, celery seed, cardamom, cinnamon, thyme and fennel). ) in a specific ratio can maintain the impact of the initial taste and the persistence of the aftertaste even when the amount of salt used is reduced.
特開2016-131560号公報JP 2016-131560 A 特開2020-74702号公報Japanese Patent Application Laid-Open No. 2020-74702 特開2017-70217号公報JP 2017-70217 A
 本発明は、上述した実情に鑑み、口に入れた瞬間に風味(呈味と香り)のインパクトが強く感じられる飲食品を提供することを課題とする。 An object of the present invention is to provide a food or drink that has a strong impact of flavor (taste and aroma) the moment it is put in the mouth, in view of the above-mentioned circumstances.
 本発明者らは上記課題を解決すべく鋭意研究を進めた結果、呈味成分とともに、酢酸テルピニル、アピゲニンまたはルテオリンのいずれか一つ以上を飲食品に一定量配合することによって、風味(呈味と香り)のインパクトが強く感じられることを見出し、本発明を完成させるに至った。 As a result of intensive research to solve the above problems, the present inventors have found that by adding a certain amount of one or more of terpinyl acetate, apigenin, and luteolin to food and drink together with a taste component, flavor (taste and fragrance), and found that the impact of the fragrance is felt strongly, leading to the completion of the present invention.
 すなわち、本発明は、以下の発明を包含する。
(1)酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上と呈味成分を含有する、呈味と香りが向上した飲食品。
(2)酢酸テルピニルの含有量が0.00000001ppm以上10ppm以下である、(1)に記載の飲食品。
(3)アピゲニンの含有量が0.00001ppm以上10ppm以下である、(1)に記載の飲食品。
(4)ルテオリンの含有量が0.00001ppm以上100ppm以下である、(1)に記載の飲食品。
(5)前記呈味成分が、うま味成分、塩味成分、甘味成分、及び酸味成分から選ばれる少なくとも1種である、(1)~(4)のいずれかに記載の飲食品。
(6)前記呈味成分が、うま味成分であって、該うま味成分が、グルタミン酸、イノシン酸、及びコハク酸から選ばれる少なくとも1種である、(1)~(5)のいずれかに記載の飲食品。
(7)前記呈味成分が、グルタミン酸又はイノシン酸のいずれか1種又は2種のうま味成分であって、以下の(a1)及び(a2)を満たす、(1)~(6)のいずれか1項に記載の飲食品。
 (a1) 飲食品中のグルタミン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/グルタミン酸の含有量(ppm))が、5.0×10-12~1×10-2
 (a2) 飲食品中のイノシン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/イノシン酸の含有量(ppm))が6.0×10-11~0.2
(8)前記呈味成分が、グルタミン酸又はイノシン酸のいずれか1種又は2種のうま味成分であって、以下の(b1)及び(b2)を満たす、(1)~(6)のいずれかに記載の飲食品。
 (b1) 飲食品中のグルタミン酸の含有量に対するアピゲニンの含有量の比率(アピゲニンの含有量(ppm)/グルタミン酸の含有量(ppm))が、5.0×10-9~1×10-2
 (b2) 飲食品中のイノシン酸の含有量に対するアピゲニンの含有量の比率(アピゲニンの含有量(ppm)/イノシン酸の含有量(ppm))が5.0×10-10~1.0×10-3
(9)前記呈味成分が、グルタミン酸又はイノシン酸のいずれか1種又は2種のうま味成分であって、以下の(c1)及び(c2)を満たす、(1)~(6)のいずれかに記載の飲食品。
 (c1) 飲食品中のグルタミン酸の含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/グルタミン酸の含有量(ppm))が、5.0×10-9~1.0×10-1
 (c2) 飲食品中のイノシン酸の含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/イノシン酸の含有量(ppm))が5.0×10-10~1.0×10-2
(10)前記飲食品が、調味料である、(1)~(9)のいずれかに記載の飲食品。
(11)前記調味料が、液状調味料である、(10)に記載の飲食品。
(12)前記調味料が、つゆ類、たれ類、だし類、エキス類、ソース類、又はドレッシング類である、(10)又は(11)に記載の飲食品。
(13)前記飲食品が、植物性蛋白質含有食品である、(1)~(9)のいずれかに記載の飲食品。
(14)酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を、呈味成分を含む飲食品又はその原料に添加することを含む、飲食品の呈味と香りを向上させる方法。
(15)下記の段階(i)~(iii)を含む方法で植物素材抽出物を調製することを含む、(1)~(13)のいずれかに記載の呈味と香りが向上した飲食品の製造方法。
 (i) 酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材を抽出溶媒に添加する段階
 (ii) 植物素材から抽出溶媒にて成分抽出し、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材抽出物を得る工程
 (iii) (ii)で得られた植物素材抽出物のpHを相対的に酸性側に調整する段階
(16)前記段階(ii)の後、植物素材抽出物のpHを6.5以上12.0以下に調整する段階をさらに含む、(15)に記載の製造方法。
(17)調製した植物素材抽出物のpHが1.5以上7.0未満である、(15)又は(16)に記載の製造方法。
That is, the present invention includes the following inventions.
(1) A food or drink with improved taste and aroma, containing one or more selected from terpinyl acetate, apigenin, and luteolin and a taste component.
(2) The food or drink according to (1), wherein the content of terpinyl acetate is 0.00000001 ppm or more and 10 ppm or less.
(3) The food or drink according to (1), wherein the apigenin content is 0.00001 ppm or more and 10 ppm or less.
(4) The food or drink according to (1), wherein the content of luteolin is 0.00001 ppm or more and 100 ppm or less.
(5) The food or drink according to any one of (1) to (4), wherein the taste component is at least one selected from an umami component, a salty component, a sweet component, and a sour component.
(6) The umami component according to any one of (1) to (5), wherein the taste component is an umami component, and the umami component is at least one selected from glutamic acid, inosinic acid, and succinic acid. food and drink.
(7) any one of (1) to (6), wherein the taste component is one or two of glutamic acid and inosinic acid, and satisfies (a1) and (a2) below; 1. Food and drink according to item 1.
(a1) The ratio of the content of terpinyl acetate to the content of glutamic acid in the food or drink (content of terpinyl acetate (ppm)/content of glutamic acid (ppm)) is 5.0×10 −12 to 1×10 -2
(a2) The ratio of the content of terpinyl acetate to the content of inosinic acid in the food or drink (content of terpinyl acetate (ppm)/content of inosinic acid (ppm)) is 6.0×10 −11 to 0.0. 2
(8) any one of (1) to (6), wherein the taste component is one or two of glutamic acid and inosinic acid, and satisfies the following (b1) and (b2); Food and drink listed in .
(b1) The ratio of the apigenin content to the glutamic acid content in the food or drink (apigenin content (ppm)/glutamic acid content (ppm)) is 5.0×10 −9 to 1×10 −2
(b2) The ratio of the content of apigenin to the content of inosinic acid in the food or drink (content of apigenin (ppm)/content of inosinic acid (ppm)) is 5.0×10 −10 to 1.0× 10-3
(9) any one of (1) to (6), wherein the taste component is one or two of glutamic acid and inosinic acid, and satisfies the following (c1) and (c2); Food and drink listed in .
(c1) The ratio of the content of luteolin to the content of glutamic acid in the food or drink (luteolin content (ppm) / glutamic acid content (ppm)) is 5.0 × 10 -9 to 1.0 × 10 -1
(c2) The ratio of the content of luteolin to the content of inosinic acid in the food or drink (luteolin content (ppm)/inosinic acid content (ppm)) is 5.0×10 −10 to 1.0× 10-2
(10) The food or drink according to any one of (1) to (9), which is a seasoning.
(11) The food or drink according to (10), wherein the seasoning is a liquid seasoning.
(12) The food or drink according to (10) or (11), wherein the seasoning is soups, sauces, soup stocks, extracts, sauces, or dressings.
(13) The food or drink according to any one of (1) to (9), which is a vegetable protein-containing food.
(14) A method for improving the taste and aroma of food and drink, which comprises adding one or more selected from terpinyl acetate, apigenin, and luteolin to food and drink containing a taste component or raw materials thereof.
(15) The food or drink with improved taste and aroma according to any one of (1) to (13), which comprises preparing a plant material extract by a method including the following steps (i) to (iii). manufacturing method.
(i) A step of adding a plant material containing one or more selected from terpinyl acetate, apigenin, and luteolin to an extraction solvent (ii) Extracting components from the plant material with an extraction solvent, and a step of obtaining a plant material extract containing one or more selected from luteolin (iii) a step of adjusting the pH of the plant material extract obtained in (ii) to a relatively acidic side (16) The production method according to (15), further comprising the step of adjusting the pH of the plant material extract to 6.5 or more and 12.0 or less after the step (ii).
(17) The production method according to (15) or (16), wherein the prepared plant material extract has a pH of 1.5 or more and less than 7.0.
 本願は、2021年7月26日に出願された日本国特許出願2021-121841号の優先権を主張するものであり、該特許出願の明細書に記載される内容を包含する。 This application claims priority from Japanese Patent Application No. 2021-121841 filed on July 26, 2021, and includes the contents described in the specification of the patent application.
 本発明によれば、口に入れた瞬間に呈味と香りを共に強く感じられる美味しい飲食品が提供される。本発明の飲食品は、メカニズムは不明だが温度や形態に関わらず、口にいれた瞬間の呈味と香りをシャープに感じることでインパクトが強く、素材本来の風味が良好で、雑味がなく、嗜好性に優れている。  According to the present invention, delicious foods and drinks that give a strong sense of both taste and aroma the moment they are put into the mouth are provided. Although the mechanism is unknown, the food and drink of the present invention have a strong impact by sharply feeling the taste and aroma at the moment they are put in the mouth, regardless of the temperature or form, and the original flavor of the material is good and there is no unpleasant taste. , good palatability.
1.呈味と香りが向上した飲食品
 本発明の呈味と香りが向上した飲食品は、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上と呈味成分を含有する。ここで、「呈味」とは、主として舌に存在する味蕾と呼ばれる器官の味覚受容体で受容され、互いに明確にできる味をいい、甘味、酸味、塩味、苦味、うま味の5基本味が含まれる。「香り」とは、飲食品を口に含んだ時や咀嚼した時に鼻によって検知される香気をいう。また、「風味」とは、呈味と香りによって構成される複合的な感覚をいい、全体的な美味しさにつながる。
1. Food and Beverage with Improved Taste and Aroma The food and drink with improved taste and aroma of the present invention contains one or more selected from terpinyl acetate, apigenin, and luteolin, and a taste component. Here, the term "taste" refers to tastes that are received by taste receptors in organs called taste buds that are mainly present on the tongue and that can be distinct from each other, and includes five basic tastes: sweet, sour, salty, bitter and umami. be "Aroma" refers to the aroma detected by the nose when food or drink is put in the mouth or chewed. Also, "flavor" refers to a complex sensation composed of taste and aroma, and is linked to overall deliciousness.
 本発明における「呈味」は、上記の5基本味のうち、主として、うま味、塩味、及び甘味をいうが、好ましくはうま味(umami)である。また、本発明における「呈味」には、飲食品を口に含んだ瞬間(例えば、2秒以内)に感じられる呈味と、飲食品を口に入れた後、飲み込むまでの間や咀嚼している間に口内に広がり、かつ持続する呈味(呈味の広がり)の両方が包含される。本発明における「香り」は、飲食品を口に含んだ瞬間(例えば、2秒以内)に感じられる呈味と連関する香りをいう。 "Taste" in the present invention mainly refers to umami, saltiness, and sweetness among the above five basic tastes, preferably umami. In addition, the "taste" in the present invention includes the taste that is felt at the moment the food or drink is put in the mouth (for example, within 2 seconds), and the taste that is felt after the food or drink is put in the mouth and before swallowing or during mastication. It includes both a taste that spreads in the mouth while being held and a taste that lasts (spread of taste). The "aroma" in the present invention refers to the aroma associated with the taste felt at the moment (for example, within 2 seconds) when the food or drink is put in the mouth.
(酢酸テルピニル)
 本発明の飲食品に用いられる酢酸テルピニル(体系名:4-メチル-1-(1-メチルエチル)-3-シクロヘキセン-1-オールアセタート、英文表記:Terpinyl acetate)は、分子式C1220(分子量:196.29)を有し、CAS登録番号は80-26-2である。
(terpinyl acetate)
Terpinyl acetate (systematic name: 4-methyl-1-(1-methylethyl)-3-cyclohexen-1-ol acetate, English notation: Terpinyl acetate) used in the food or drink of the present invention has the molecular formula C 12 H 20 O 2 (molecular weight: 196.29) and CAS registry number 80-26-2.
(アピゲニン)
 本発明の飲食品に用いられるアピゲニン(体系名:5,7-ジヒドロキシ-2-(4-ヒドロキシフェニル)-4H-1-ベンゾピラン-4-オン、英文表記:Apigenine)は、分子式C1510(分子量:270.24)を有し、CAS登録番号は520-36-5である。
(Apigenin)
Apigenin (systematic name: 5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one, English notation: Apigenine) used in the food or drink of the present invention has the molecular formula C 15 H 10 O 5 (molecular weight: 270.24) and CAS registry number 520-36-5.
(ルテオリン)
 本発明の飲食品に用いられるルテオリン(体系名:2-(3,4-ジヒドロキシフェニル)- 5,7-ジヒドロキシ-4-クロメノン、英文表記:Luteolin)は、分子式C1510(分子量:286.24)を有し、CAS登録番号は491-70-3である。
(luteolin)
Luteolin (systematic name: 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-chromenone, English notation: Luteolin) used in the food or drink of the present invention has the molecular formula C 15 H 10 O 6 (molecular weight : 286.24) and has a CAS registry number of 491-70-3.
 本発明における酢酸テルピニル、アピゲニン、又はルテオリンは、経口摂取できるものであれば制限はなく、合成品であっても、酢酸テルピニル、アピゲニン、又はルテオリンを含む植物素材由来のものであってもよいが、飲食品の風味等への影響の観点から、合成品又は植物素材加工品を用いることが好ましく、合成品又は植物素材抽出物を用いることがより好ましい。ここで、酢酸テルピニルを含む植物素材としては、例えば、セロリ、ローリエ、パセリ、カルダモン、月桂樹などが挙げられ、アピゲニン、ルテオリンを含む植物素材としては、例えば、セロリ、パセリなどが挙げられる。これらは単独で用いてもよく、2種以上を組み合わせて用いてもよい。植物素材加工品とは、酢酸テルピニル、アピゲニン、又はルテオリンを含む植物素材の乾燥、粉砕、抽出、精製等の処理品をいう。例えば、加工品の好ましい態様である抽出物は、上記の酢酸テルピニルを含有する植物素材を溶媒に添加し、静置した後、ろ過を行うことによって調製できる。あるいは、上記の酢酸テルピニル、アピゲニン、又はルテオリンを含有する植物素材を添加した溶媒を特定の温度に加熱した後静置する、又は、攪拌しながら一定温度で保持することによっても調製できる。ここで、抽出に用いる溶媒としては、中性の溶媒として、水、エタノール、又は水-エタノールの混合溶媒が挙げられるが、水が好ましい。 Terpinyl acetate, apigenin, or luteolin in the present invention is not limited as long as it can be orally ingested, and may be synthetic or derived from plant materials containing terpinyl acetate, apigenin, or luteolin. From the viewpoint of the influence on the flavor of food and drink, it is preferable to use a synthetic product or a plant material processed product, and it is more preferable to use a synthetic product or a plant material extract. Examples of plant materials containing terpinyl acetate include celery, bay leaf, parsley, cardamom, and laurel, and examples of plant materials containing apigenin and luteolin include celery and parsley. These may be used alone or in combination of two or more. The plant material processed product refers to a product processed by drying, pulverizing, extracting, refining, etc. of a plant material containing terpinyl acetate, apigenin, or luteolin. For example, an extract, which is a preferred embodiment of the processed product, can be prepared by adding the terpinyl acetate-containing plant material to a solvent, allowing the mixture to stand, and then filtering. Alternatively, it can be prepared by heating a solvent to which the plant material containing terpinyl acetate, apigenin, or luteolin has been added and allowing it to stand after being heated to a specific temperature, or by maintaining it at a constant temperature while stirring. Here, the solvent used for extraction includes water, ethanol, and a mixed solvent of water-ethanol as a neutral solvent, with water being preferred.
 また、抽出に用いる溶媒のpHを所定の範囲とすることで、酢酸テルピニル、アピゲニン、ルテオリンが抽出しやすくなるため好ましい。具体的には、酢酸テルピニル、アピゲニン、又はルテオリンの少なくとも1種を含有する植物素材を抽出溶媒に添加するとともに、抽出溶媒のpHを室温で6.5以上11.0以下に調整して成分抽出してもよい。抽出溶媒のpHは6.5以上、7.0以上、7.5以上、8.0以上、9.0以上であってもよく、その上限は特に制限されないが、通常11.0以下、10.5以下、10.0以下であってもよい。 In addition, it is preferable to set the pH of the solvent used for extraction within a predetermined range, because it facilitates the extraction of terpinyl acetate, apigenin, and luteolin. Specifically, a plant material containing at least one of terpinyl acetate, apigenin, or luteolin is added to the extraction solvent, and the pH of the extraction solvent is adjusted to 6.5 or more and 11.0 or less at room temperature to extract the components. You may The pH of the extraction solvent may be 6.5 or higher, 7.0 or higher, 7.5 or higher, 8.0 or higher, or 9.0 or higher. 0.5 or less, or 10.0 or less.
 また、本発明において、酢酸テルピニル、アピゲニン、又はルテオリンの少なくとも1種を含有する植物素材を抽出溶媒に添加した後に抽出溶媒のpHを調整してもよく、予めpHが所定範囲に調整された抽出溶媒に、酢酸テルピニル、アピゲニン、又はルテオリンの少なくとも1種を含有する植物素材を添加してもよい。 Further, in the present invention, the pH of the extraction solvent may be adjusted after adding the plant material containing at least one of terpinyl acetate, apigenin, or luteolin to the extraction solvent. A plant material containing at least one of terpinyl acetate, apigenin, or luteolin may be added to the solvent.
 pHの調整に際しては、抽出溶媒にpH調整剤(例えば水酸化ナトリウム、水酸化カリウム、炭酸ナトリウム、炭酸カリウム、炭酸カルシウム、炭酸水素ナトリウム、グルコン酸カリウム、乳酸、クエン酸、酒石酸、リンゴ酸及び酢酸等)を溶解させることでpHを調整することができるが、特に炭酸塩である炭酸ナトリウム、炭酸カリウム又は炭酸カルシウムを用いることが好ましい。 When adjusting the pH, the extraction solvent may be added with a pH adjuster (e.g. sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, potassium gluconate, lactic acid, citric acid, tartaric acid, malic acid and acetic acid). etc.) can be dissolved, but it is particularly preferable to use carbonates such as sodium carbonate, potassium carbonate, or calcium carbonate.
 さらに、エタノール以外のアルコール類や超臨界状態の二酸化炭素を用いても抽出可能である。 Furthermore, it is possible to extract using alcohols other than ethanol or carbon dioxide in a supercritical state.
 溶媒の使用量については、特に限定はなく、例えば上記酢酸テルピニル、アピゲニン、又はルテオリンを含む植物素材(乾燥重量)に対し、10倍以上、好ましくは20倍以上であればよいが、抽出後に濃縮を行ったり、単離したりする場合の操作の便宜上100倍以下であることが好ましい。酢酸テルピニル、アピゲニン、又はルテオリンの抽出物は、そのまま本発明の飲食品に用いることができるが、必要に応じて濃縮処理、熱風乾燥、蒸気乾燥、凍結乾燥、噴霧乾燥等の乾燥処理、分離精製処理、脱色処理等に供して、濃縮物や乾燥物等にしたものを用いてもよい。 The amount of the solvent to be used is not particularly limited. For example, it may be 10 times or more, preferably 20 times or more, the plant material (dry weight) containing terpinyl acetate, apigenin, or luteolin, but it may be concentrated after extraction. or isolation, it is preferably 100 times or less for convenience of operation. The extract of terpinyl acetate, apigenin, or luteolin can be used as it is in the food or drink of the present invention, but if necessary, it may be subjected to concentration treatment, hot air drying, steam drying, freeze drying, drying treatment such as spray drying, separation and purification. It may be subjected to treatment, decolorization treatment, etc., and used as a concentrate, dried product, or the like.
 本発明の飲食品における、酢酸テルピニルの含有量(ppm)は、飲食品の種類にもよるが、0.00000001ppm以上、好ましくは0.000001ppm以上、より好ましくは0.0001ppm以上であり、かつ、10ppm以下、好ましくは1ppm以下、より好ましくは0.01ppm以下である。酢酸テルピニルの含有量(ppm)が0.00000001ppmよりも少ないと、風味のインパクト及び呈味の広がりなく、全体の美味しさがない。酢酸テルピニルの含有量(ppm)が10ppmよりも多いと、風味のインパクト及び呈味の広がりなく、全体の美味しさがない。本発明において、「風味のインパクト」とは、口に入れた瞬間に呈味と香りの向上が強く感じられることを表す。 The content (ppm) of terpinyl acetate in the food or drink of the present invention depends on the type of the food or drink, but is 0.00000001 ppm or more, preferably 0.000001 ppm or more, more preferably 0.0001 ppm or more, and It is 10 ppm or less, preferably 1 ppm or less, more preferably 0.01 ppm or less. If the content (ppm) of terpinyl acetate is less than 0.00000001 ppm, the impact of the flavor and the spread of the taste will not be achieved, and the taste as a whole will be lost. If the content (ppm) of terpinyl acetate is more than 10 ppm, the impact of the flavor and the spread of the taste will not be achieved, and the taste as a whole will be lost. In the present invention, the term "flavor impact" means that a strong sense of improvement in taste and aroma is felt the moment the food is put in the mouth.
 なお、本発明の飲食品に含まれる酢酸テルピニルは、飲食品の原料となる食用植物等の食材に含まれるものでもよく、本発明の飲食品の製造時に、当該食材とは別に添加されるものでもよく、本発明の飲食品の製造に伴い生じるものであってもよい。或いはこれらのうち2種以上の由来による酢酸テルピニルが合計された結果として、前記の所定の含有量及び/又は割合を満たすものであってもよい。本発明の飲食品の製造時に外部から酢酸テルピニルを添加する場合、精製抽出された高純度の酢酸テルピニル試薬を添加してもよく、酢酸テルピニルを含む何らかの植物素材加工品(例えば抽出物)の形態で添加してもよい。但し、当該飲食品に含有される酢酸テルピニルの過半(より好ましくは全部)が何らかの食材由来であることが好ましく、食用植物由来であることがより好ましい。 The terpinyl acetate contained in the food and drink of the present invention may be contained in food materials such as edible plants that are raw materials of the food and drink, and are added separately from the food and food when the food and drink of the present invention are produced. However, it may be generated along with the production of the food or drink of the present invention. Alternatively, terpinyl acetate derived from two or more of these may be combined to satisfy the above predetermined content and/or ratio. When terpinyl acetate is added externally during the production of the food or drink of the present invention, a highly pure terpinyl acetate reagent that has been purified and extracted may be added, or in the form of some processed plant material (e.g., extract) containing terpinyl acetate. may be added at However, the majority (more preferably all) of terpinyl acetate contained in the food or drink is preferably derived from some kind of foodstuff, more preferably derived from edible plants.
 本発明の飲食品における、アピゲニンの含有量(ppm)は、飲食品の種類にもよるが、0.00001ppm以上、好ましくは0.0001ppm以上、より好ましくは0.001ppm以上であり、かつ、10ppm以下、好ましくは1ppm以下、より好ましくは0.1ppm以下である。アピゲニンの含有量(ppm)が0.00001ppmよりも少ないと、風味のインパクト及び呈味の広がりなく、全体の美味しさがない。アピゲニンの含有量(ppm)が10ppmよりも多いと、風味のインパクト及び呈味の広がりなく、全体の美味しさがない。 The content (ppm) of apigenin in the food or drink of the present invention depends on the type of food or drink, but is 0.00001 ppm or more, preferably 0.0001 ppm or more, more preferably 0.001 ppm or more, and 10 ppm. Below, preferably 1 ppm or less, more preferably 0.1 ppm or less. If the content (ppm) of apigenin is less than 0.00001 ppm, the impact of the flavor and the spread of the taste will not be obtained, and the overall deliciousness will be lost. If the apigenin content (ppm) is more than 10 ppm, the impact of the flavor and the spread of the taste are not obtained, and the overall taste is not good.
 本発明の飲食品に含まれるアピゲニンは、飲食品の原料となる食用植物等の食材に含まれるものでもよく、本発明の飲食品の製造時に、当該食材とは別に添加されるものでもよく、本発明の飲食品の製造に伴い生じるものであってもよい。或いはこれらのうち2種以上の由来によるアピゲニンが合計された結果として、前記の所定の含有量及び/又は割合を満たすものであってもよい。本発明の飲食品の製造時に外部からアピゲニンを添加する場合、精製抽出された高純度のアピゲニン試薬を添加してもよく、アピゲニンを含む何らかの植物素材加工品(例えば抽出物)の形態で添加してもよい。但し、当該飲食品に含有されるアピゲニンの過半(より好ましくは全部)が何らかの食材由来であることが好ましく、食用植物由来であることがより好ましい。 The apigenin contained in the food and drink of the present invention may be contained in food materials such as edible plants that are raw materials of the food and drink, or may be added separately from the food and food when the food and drink of the present invention are produced. It may occur along with the production of the food or drink of the present invention. Alternatively, the predetermined content and/or ratio may be satisfied as a result of combining apigenin derived from two or more of these. When apigenin is added externally during the production of the food or drink of the present invention, a high-purity apigenin reagent that has been purified and extracted may be added. may However, the majority (more preferably all) of the apigenin contained in the food or drink is preferably derived from some kind of foodstuff, and more preferably derived from edible plants.
 本発明の飲食品における、ルテオリンの含有量(ppm)は、飲食品の種類にもよるが、0.00001ppm以上、好ましくは0.0001ppm以上、より好ましくは0.001ppm以上であり、かつ、100ppm以下、好ましくは1ppm以下、より好ましくは0.1ppm以下である。ルテオリンの含有量(ppm)が0.00001ppmよりも少ないと、風味のインパクト及び呈味の広がりなく、全体の美味しさがない。ルテオリンの含有量(ppm)が100ppmよりも多いと、風味のインパクト及び呈味の広がりなく、全体の美味しさがない。 The content (ppm) of luteolin in the food and drink of the present invention is 0.00001 ppm or more, preferably 0.0001 ppm or more, more preferably 0.001 ppm or more, and is 100 ppm, depending on the type of food and drink. Below, preferably 1 ppm or less, more preferably 0.1 ppm or less. If the content (ppm) of luteolin is less than 0.00001 ppm, there is no flavor impact and no spread of taste, and there is no overall deliciousness. If the content (ppm) of luteolin is more than 100 ppm, the flavor impact and the spread of the taste are not obtained, and the overall deliciousness is lost.
 なお、本発明の飲食品に含まれるルテオリンは、飲食品の原料となる食用植物等の食材に含まれるものでもよく、本発明の飲食品の製造時に、当該食材とは別に添加されるものでもよく、本発明の飲食品の製造に伴い生じるものであってもよい。或いはこれらのうち2種以上の由来によるルテオリンが合計された結果として、前記の所定の含有量及び/又は割合を満たすものであってもよい。本発明の飲食品の製造時に外部からルテオリンを添加する場合、精製抽出された高純度のルテオリン試薬を添加してもよく、ルテオリンを含む何らかの植物素材加工品(例えば抽出物)の形態で添加してもよい。但し、当該飲食品に含有されるルテオリンの過半(より好ましくは全部)が何らかの食材由来であることが好ましく、食用植物由来であることがより好ましい。 The luteolin contained in the food and drink of the present invention may be contained in food materials such as edible plants that are raw materials of the food and drink, or may be added separately from the food and food when the food and drink of the present invention are produced. Well, it may be generated along with the production of the food or drink of the present invention. Alternatively, the predetermined content and/or ratio may be satisfied as a result of combining luteolin derived from two or more of these sources. When luteolin is added externally during the production of the food or drink of the present invention, a high-purity luteolin reagent that has been purified and extracted may be added. may However, the majority (more preferably all) of the luteolin contained in the food or drink is preferably derived from some kind of foodstuff, more preferably derived from edible plants.
 本発明の飲食品が酢酸テルピニル及びアピゲニンを含有する場合、酢酸テルピニルの含有量に対するアピゲニンの含有量の比率(アピゲニンの含有量(ppm)/酢酸テルピニルの含有量(ppm)は、例えば0.01~100,000、0.1~100,000、0.1~10,000、1~10,000、1~5,000、又は1~1,000である。 When the food or drink of the present invention contains terpinyl acetate and apigenin, the ratio of the apigenin content to the terpinyl acetate content (apigenin content (ppm)/terpinyl acetate content (ppm) is, for example, 0.01 ~100,000, 0.1-100,000, 0.1-10,000, 1-10,000, 1-5,000, or 1-1,000.
 本発明の飲食品が酢酸テルピニル及びルテオリンを含有する場合、酢酸テルピニルの含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/酢酸テルピニルの含有量(ppm)は、例えば0.0001~100,000、0.001~100,000、0.001~10,000、0.01~10,000、0.01~5,000、又は0.01~1,000である。 When the food or drink of the present invention contains terpinyl acetate and luteolin, the ratio of the luteolin content to the terpinyl acetate content (luteolin content (ppm)/terpinyl acetate content (ppm) is, for example, 0.0001 ~100,000, 0.001 to 100,000, 0.001 to 10,000, 0.01 to 10,000, 0.01 to 5,000, or 0.01 to 1,000.
 本発明の飲食品がアピゲニン及びルテオリンを含有する場合、アピゲニンの含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/アピゲニンの含有量(ppm)は、例えば0.0001~100、0.001~100、0.001~10、0.005~10、0.005~5、0.01~1である。 When the food or drink of the present invention contains apigenin and luteolin, the ratio of the content of luteolin to the content of apigenin (content of luteolin (ppm)/content of apigenin (ppm) is, for example, 0.0001 to 100, 0.001-100, 0.001-10, 0.005-10, 0.005-5, 0.01-1.
 また本発明の飲食品が酢酸テルピニル、アピゲニン及びルテオリンを含有する場合、酢酸テルピニルの含有量に対するアピゲニンとルテオリンの合計含有量の比率(アピゲニン+ルテオリンの含有量(ppm)/酢酸テルピニルの含有量(ppm)は、例えば0.01~100,000、0.1~100,000、0.1~10,000、1~10,000、1~5,000、1~2,000である。 When the food or drink of the present invention contains terpinyl acetate, apigenin and luteolin, the ratio of the total content of apigenin and luteolin to the content of terpinyl acetate (content of apigenin + luteolin (ppm) / content of terpinyl acetate ( ppm) is, for example, 0.01 to 100,000, 0.1 to 100,000, 0.1 to 10,000, 1 to 10,000, 1 to 5,000, 1 to 2,000.
(呈味成分)
 本発明の飲食品に用いられる「呈味成分」には、アミノ酸や核酸などのうま味成分、糖類などの甘味成分、有機酸などの酸味成分、及び食塩その他の塩類などの塩味成分が包含されるが、うま味成分が好ましい。うま味成分としては、アミノ酸系、核酸系、又は有機酸系のいずれのうまみ成分でよい。例えば、アミノ酸系うま味成分としては、グルタミン酸、アスパラギン酸、オキシグルタミン酸、リジン、ヒスチジン、タウリン、ベタイン、グリシン、クレアチン、アラニン、アルギニン等又はそれらの塩が挙げられ、核酸系うま味成分としては、イノシン酸、グアニル酸、又はそれらの塩が挙げられ、有機酸系うま味成分としては、コハク酸、酢酸、クエン酸、リンゴ酸又はそれらの塩が挙げられる。また、うま味成分は、上記の各成分を含む食材又はその加工品を使用してもよい。例えば、うま味成分を含む食品又はその加工品としては、野菜類(例えば、ニンジン、タマネギ、セロリ、ハクサイ、キャベツ等)の粉末及びそれらのエキス、海藻類(例えば、コンブ、ワカメ等)の粉末及びそれらのエキス、キノコ類(例えば、シイタケ、シメジ等)の粉末及びそれらのエキス、魚介類(例えば、カツオ、サバ、アジ、イワシ、エビ、ホタテ、カキ等)の粉末及びそれらのエキス、畜肉(例えば、牛肉、豚肉、鶏肉等)の粉末及びそれらのエキス等が挙げられる。
(Taste component)
The "taste component" used in the food or drink of the present invention includes umami components such as amino acids and nucleic acids, sweet components such as sugars, sour components such as organic acids, and salty components such as common salt and other salts. However, the umami component is preferred. The umami component may be an amino acid-based, nucleic acid-based, or organic acid-based umami component. For example, amino acid-based umami components include glutamic acid, aspartic acid, oxyglutamic acid, lysine, histidine, taurine, betaine, glycine, creatine, alanine, arginine, and salts thereof, and nucleic acid-based umami components include inosinic acid. , guanylic acid, or salts thereof, and organic acid-based umami components include succinic acid, acetic acid, citric acid, malic acid, or salts thereof. Also, the umami component may be a food material containing each of the above components or a processed product thereof. For example, foods containing umami components or processed products thereof include powders and extracts of vegetables (e.g., carrots, onions, celery, Chinese cabbage, cabbage, etc.), powders of seaweeds (e.g., kelp, wakame, etc.) and Their extracts, mushrooms (e.g., shiitake, shimeji, etc.) powders and their extracts, seafood (e.g., bonito, mackerel, horse mackerel, sardines, shrimp, scallops, oysters, etc.) powders and their extracts, meat ( Examples include beef, pork, chicken, etc.) powders and extracts thereof.
 本発明の飲食品における、グルタミン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/グルタミン酸の含有量(ppm))は、風味のインパクト及び呈味の広がりの十分な効果の観点等から、通常5.0×10-12~1×10-2であり、好ましくは5.0×10-10~6.0×10-3、より好ましくは5.0×10-9~6.0×10-4である。 The ratio of the content of terpinyl acetate to the content of glutamic acid (terpinyl acetate content (ppm)/glutamic acid content (ppm)) in the food and drink of the present invention is sufficient for flavor impact and spread of taste. From the viewpoint of effects, etc., it is usually 5.0×10 −12 to 1×10 −2 , preferably 5.0×10 −10 to 6.0×10 −3 , more preferably 5.0×10 −3 . 9 to 6.0×10 −4 .
 本発明の飲食品中のグルタミン酸の含有量は、本発明の効果を発揮できる限りにおいて、特に制限されないが、500ppm以上、好ましくは750ppm以上、より好ましくは1000ppm以上であり、上限は通常20000ppm以下、好ましくは15000ppm以下、より好ましくは12000m以下、さらに好ましくは5000ppm以下、又は4000ppm以下、又は3000ppm以下である。 The content of glutamic acid in the food and drink of the present invention is not particularly limited as long as the effect of the present invention can be exhibited, but it is 500 ppm or more, preferably 750 ppm or more, more preferably 1000 ppm or more, and the upper limit is usually 20000 ppm or less. It is preferably 15000 ppm or less, more preferably 12000 ppm or less, still more preferably 5000 ppm or less, or 4000 ppm or less, or 3000 ppm or less.
 本発明の飲食品における、イノシン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/イノシン酸の含有量(ppm))は、風味のインパクト及び呈味の広がりの十分な効果の観点等から、通常6.0×10-11~0.2であり、好ましくは6.0×10-9~0.1であり、より好ましくは6.0×10-8~0.01である。 The ratio of the terpinyl acetate content to the inosinic acid content (terpinyl acetate content (ppm)/inosinic acid content (ppm)) in the food or drink of the present invention determines the impact of flavor and spread of taste. From the viewpoint of sufficient effect, etc., it is usually 6.0×10 −11 to 0.2, preferably 6.0×10 −9 to 0.1, more preferably 6.0×10 −8 to 0.01.
 本発明の飲食品中のイノシン酸の含有量は、本発明の効果を発揮できる限りにおいて、特に制限されないが、10ppm以上、好ましくは15ppm以上、より好ましくは100ppm以上であり、上限は通常2000ppm以下、好ましくは1500ppm以下、より好ましくは1200ppm以下、さらに好ましくは500ppm以下、又は400ppm以下、又は300ppm以下である。 The content of inosinic acid in the food and drink of the present invention is not particularly limited as long as the effect of the present invention can be exhibited, but is 10 ppm or more, preferably 15 ppm or more, more preferably 100 ppm or more, and the upper limit is usually 2000 ppm or less. , preferably 1500 ppm or less, more preferably 1200 ppm or less, still more preferably 500 ppm or less, or 400 ppm or less, or 300 ppm or less.
 本発明の飲食品における、グルタミン酸の含有量に対するアピゲニンの含有量の比率(アピゲニンの含有量(ppm)/グルタミン酸の含有量(ppm))は、風味のインパクト及び呈味の広がりの十分な効果の観点等から、通常5.0×10-9~1.0×10-2であり、好ましくは4.0×10-8~1.0×10-3である。 The ratio of the content of apigenin to the content of glutamic acid (apigenin content (ppm)/glutamic acid content (ppm)) in the food or drink of the present invention is sufficient for the impact of flavor and the spread of taste. From the point of view, it is usually 5.0×10 −9 to 1.0×10 −2 , preferably 4.0×10 −8 to 1.0×10 −3 .
 本発明の飲食品における、イノシン酸の含有量に対するアピゲニンの含有量の比率(アピゲニンの含有量(ppm)/イノシン酸の含有量(ppm))は、風味のインパクト及び呈味の広がりの十分な効果の観点等から、通常5.0×10-10~1.0×10-3であり、好ましくは5.0×10-8~1.0×10-4である。 The ratio of the content of apigenin to the content of inosinic acid (apigenin content (ppm)/inosinic acid content (ppm)) in the food or drink of the present invention is sufficient for flavor impact and spread of taste. From the viewpoint of effect, etc., it is usually 5.0×10 −10 to 1.0×10 −3 , preferably 5.0×10 −8 to 1.0×10 −4 .
 本発明の飲食品における、グルタミン酸の含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/グルタミン酸の含有量(ppm))は、風味のインパクト及び呈味の広がりの十分な効果の観点等から、通常5.0×10-9~1.0×10-1であり、好ましくは5.0×10-7~1.0×10-2である。 The ratio of the content of luteolin to the content of glutamic acid (luteolin content (ppm)/glutamic acid content (ppm)) in the food and drink of the present invention is sufficient for the impact of flavor and the spread of taste. From the point of view, etc., it is usually 5.0×10 −9 to 1.0×10 −1 , preferably 5.0×10 −7 to 1.0×10 −2 .
 本発明の飲食品における、イノシン酸の含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/イノシン酸の含有量(ppm))は、風味のインパクト及び呈味の広がりの十分な効果の観点等から、通常5.0×10-10~1.0×10-2であり、好ましくは5.0×10-8~1.0×10-3である。 The ratio of the content of luteolin to the content of inosinic acid (luteolin content (ppm)/inosinic acid content (ppm)) in the food and drink of the present invention is sufficient for flavor impact and spread of taste. From the viewpoint of effect, etc., it is usually 5.0×10 −10 to 1.0×10 −2 , preferably 5.0×10 −8 to 1.0×10 −3 .
 本発明の飲食品中の酢酸テルピニル、アピゲニン、ルテオリン、グルタミン酸、イノシン酸の各成分の濃度は、その成分の配合量が明らかである場合(例えば、飲食品が精製された各成分を混合することにより得られたものである場合等)はその配合量及び飲食品の容量から算出することができ、その成分の配合量が不明である場合は、後述の参考例に記載の方法に従って又は準じて算出することができる。なお、本明細書中の「ppm」は、質量濃度(w/w)を意味する。 The concentration of each component of terpinyl acetate, apigenin, luteolin, glutamic acid, and inosinic acid in the food and drink of the present invention can be determined when the amount of the component to be added is clear (for example, when the food and drink are mixed with each refined component). can be calculated from the blending amount and the volume of the food or drink, and if the blending amount of the component is unknown, follow or follow the method described in the reference examples below. can be calculated. In addition, "ppm" in this specification means mass concentration (w/w).
(飲食品)
 本発明において「飲食品」とは、そのまま喫食に供される飲食品、及び喫食に供される飲食品を調製するための組成物(すなわち、そのまま喫食に供される飲食品の調製に使用するための組成物)の両方を意味する。
(food and drink)
In the present invention, the term "food and drink" refers to food and drink that can be eaten as it is, and compositions for preparing food and drink that are used to prepare food and drink that are used as they are. composition for).
 「そのまま喫食に供される飲食品」には、何らの成分も添加されずに喫食される飲食品のほか、喫食者が喫食時に必要に応じて調味料を適宜添加して喫食される飲食品も包含される。 “Food and drink that can be eaten as is” includes food and drink that are eaten without adding any ingredients, as well as food and drink that are eaten by adding seasonings as necessary by the person who eats them. is also included.
 「そのまま喫食に供される飲食品」としては、呈味と香りの向上が求められる飲食品であれば、特に限定はされないが、例えば飲料、菓子類、米飯類、麺類、惣菜、汁物(スープ)、パン類、ピザ、シリアル類、弁当、植物性蛋白質含有食品等が挙げられる。 "Food and drink that can be eaten as it is" is not particularly limited as long as it is a food and drink that requires improvement in taste and aroma. ), breads, pizzas, cereals, lunch boxes, vegetable protein-containing foods, and the like.
 飲料としては、例えば果汁含有飲料[例えば柑橘類(みかん、オレンジ、レモン、ライム、グレープフルーツ、柚子、カボス、スダチ、ベルガモット、ピンクグレープフルーツ、八朔、カラマンシー等)、熱帯果実(パイナップル、バナナ、グアバ、マンゴー、アセロラ、パパイヤ、パッションフルーツ等)、ライチ、イチゴ、リンゴ、モモ、ブドウ、白ブドウ、カシス、ラズベリー、ザクロ、ウメ、梨、杏、スモモ、キウイフルーツ、メロン、ブルーベリー、アサイー等のフルーツジュース、レモネード等のエード、美容系飲料、スムージー等];乳製品含有飲料(例えば乳等の乳及びその加工品である脱脂粉乳や全脂粉乳、濃縮乳、ヨーグルト、生クリーム、練乳、バター、脱脂乳、クリームパウダー、加糖粉乳、調製粉乳、ホエイパウダー、バターミルクパウダー等の乳成分を含む飲料);野菜飲料(例えばトマト、ニンジン、かぼちゃ等のジュース、スムージー、青汁等);清涼飲料水(例えばスポーツドリンク、ニアウォーター、果実風味ドリンク)、炭酸飲料;ゼリー飲料;穀物飲料(例えば米、豆乳、アーモンド等を主原料とする穀物飲料);茶系飲料[例えば紅茶、ウーロン茶、緑茶、黒茶、抹茶、ジャスミン茶、ローズヒップ茶、カモミール茶、ほうじ茶、ブレンド茶(はと麦、大麦、玄米、大豆、とうもろこし等の穀物、柿の葉、びわの葉、クマ笹、アマチャヅル、アシタバ、ドクダミ等の葉、昆布、ベニバナ、しいたけ、レイシ等をブレンドした茶)];コーヒー飲料;粉末飲料(例えばココア、青汁等);酒(例えばビール、発泡酒等のビールテイスト飲料、果実酒、日本酒等の醸造酒、焼酎、ウイスキー、ブランデー、スピリッツ等の蒸留酒、蒸留酒に糖類等の副原料を混合するリキュール等の混成酒、さらにこれら酒類に果汁やフレーバー、炭酸ガス等を加えたカクテル、フィズ、チューハイ等)等が挙げられる。 Beverages include, for example, fruit juice-containing beverages [for example, citrus fruits (mandarin orange, orange, lemon, lime, grapefruit, yuzu, kabosu, sudachi, bergamot, pink grapefruit, hassaku, calamansi, etc.), tropical fruits (pineapple, banana, guava, mango, acerola, papaya, passion fruit, etc.), lychee, strawberry, apple, peach, grape, white grape, cassis, raspberry, pomegranate, plum, pear, apricot, plum, kiwifruit, melon, blueberry, fruit juice such as acai, lemonade Etc., beauty drinks, smoothies, etc.]; Dairy-containing beverages (for example, milk and its processed products such as skim milk powder and whole milk powder, concentrated milk, yogurt, fresh cream, condensed milk, butter, skim milk, Cream powder, sweetened milk powder, modified milk powder, whey powder, buttermilk powder, and other dairy-containing beverages); vegetable drinks (e.g. juices such as tomatoes, carrots, and pumpkins; drinks, near water, fruit-flavored drinks), carbonated drinks; jelly drinks; , jasmine tea, rosehip tea, chamomile tea, roasted tea, blended tea (grains such as adlay, barley, brown rice, soybeans, corn, persimmon leaves, loquat leaves, bamboo grass, Gynostemma pentaphyllum, Angelica keiskei, Houttuynia cordata leaves, etc. , tea blended with kelp, safflower, shiitake mushrooms, litchi, etc.)]; coffee beverages; powdered beverages (e.g. cocoa, green juice, etc.); Distilled liquor such as liquor, shochu, whiskey, brandy, and spirits, mixed liquor such as liqueur, which is a mixture of distilled liquor with auxiliary ingredients such as sugars, cocktails, fizz, and chuhai that are made by adding fruit juice, flavor, carbon dioxide gas, etc. to these liquors etc.).
 菓子類としては、例えば、ゼリー、プディング、チョコレート、バー(スナックバー)、冷菓(アイスクリーム、シャーベット等)等が挙げられ、これらの中でも、ゼリー、冷菓(アイスクリーム、シャーベット等)が好ましい。 Confectioneries include, for example, jelly, pudding, chocolate, bars (snack bars), frozen desserts (ice cream, sherbet, etc.), and among these, jelly and frozen desserts (ice cream, sherbet, etc.) are preferred.
 米飯類としては、例えば、白飯、塩飯、赤飯、おこわ、炊き込み御飯、混ぜ込みご飯、おにぎり、寿司飯、餅、団子等が挙げられる。なお、「米」は、粳米、もち米や、精米度の異なる無洗米や、玄米等が挙げられる。また、これらの米飯類と他の食材との調理品でもよく、例えば寿司、ちらし寿司、カレーライス、丼物、チャーハン、天津飯等が挙げられる。 Examples of cooked rice include white rice, salted rice, red rice, steamed rice, mixed rice, mixed rice, rice balls, sushi rice, mochi, and dumplings. Note that "rice" includes glutinous rice, glutinous rice, non-washed rice with different degrees of rice polishing, brown rice, and the like. Cooked products of these rice products and other ingredients may also be used, such as sushi, chirashizushi, curry rice, rice bowls, fried rice, tenshinhan, and the like.
 麺類とは、小麦粉、米粉、そば粉、マメ等の穀類の粉を主原料とし、麺状や板状やリボン状等に成形、加工したものを、茹でたり、煮たり、蒸煮したりすることで調理される食品をいう。麺類としては、例えば、そば、うどん、きしめん、ラーメン、中華麺、パスタ、マカロニ、素麺、フォー、韓国冷麺、春雨等が挙げられる。 Noodles are made mainly from cereal flour such as wheat flour, rice flour, buckwheat flour, and beans, and are shaped into noodles, plates, ribbons, etc., and then boiled, stewed, or steamed. food that is cooked in Examples of noodles include soba, udon, kishimen, ramen, Chinese noodles, pasta, macaroni, somen, pho, Korean cold noodles, vermicelli, and the like.
 惣菜とは、肉類、魚介類、卵、乳、野菜、果物、ハーブ、海藻等の具材を適当な方法で調理して得られた食品をいう。惣菜としては、例えば、漬物、煮物、焼き物、揚げ物、炒め物、蒸し物、和え物等が挙げられる。 Prepared food refers to foods obtained by cooking ingredients such as meat, seafood, eggs, milk, vegetables, fruits, herbs, and seaweed using appropriate methods. Side dishes include, for example, pickles, boiled foods, grilled foods, fried foods, stir-fried foods, steamed foods, and seasoned foods.
 汁物(スープ)とは、肉類、魚介類、卵、乳、野菜、果物、ハーブ、海藻等の具材を適当な方法で調理して得られる、水を多く含む食品をいう。汁物(スープ)としては、例えば、味噌汁、お吸い物、ポタージュ、コンソメスープ、カレースープ、ワカメスープ、卵スープ等が挙げられる。 "Soup" refers to food containing a lot of water, obtained by cooking ingredients such as meat, seafood, eggs, milk, vegetables, fruits, herbs, and seaweed in an appropriate manner. Examples of soups include miso soup, soup, potage, consommé soup, curry soup, wakame seaweed soup, and egg soup.
 パン類とは、小麦粉又はこれに穀粉類を加えたものを主原料とし、これにイーストを加えたもの又はこれらに水、食塩、ぶどう等の果実、野菜、卵及びその加工品、砂糖類、食用油脂、乳及び乳製品等を加えたものを練り合わせ、発酵させたもの(以下「パン生地」という。)を焼いた、水分が10%以上の食品である。 Bread consists mainly of wheat flour or wheat flour added with yeast, or water, salt, fruits such as grapes, vegetables, eggs and their processed products, sugars, It is a food with a moisture content of 10% or more, which is made by kneading and fermenting edible oil, milk, dairy products, etc. (hereinafter referred to as "bread dough").
 ピザとは、小麦粉、水、塩、イースト、砂糖、少量のオリーブ油をこねた後に発酵させて作った生地を丸く薄くのばし、その上に具を乗せ、オーブンや専用の窯などで焼いた食品である。 Pizza is a food that is made by kneading flour, water, salt, yeast, sugar, and a small amount of olive oil, then fermenting the dough, rolling it out thinly, putting ingredients on it, and baking it in an oven or a special kiln. be.
 植物性蛋白質含有食品とは、植物性蛋白質を豊富に含む原料から作られる飲食品を指し、特に豆類加工品が好適である。原料となる豆類としては、例えば大豆、エンドウ、緑豆、ヒヨコ豆、落花生、ルピナス、キマメ、ナタ豆、ツル豆、インゲン豆、小豆、ササゲ、レンズ豆、ソラ豆、イナゴ豆などの全粒物やその粉砕物が挙げられ、これらから油脂や澱粉を工業的に抽出した粕を用いることもできる。飲食品がこれらの植物性蛋白質含有食品である場合、本発明の具体的な植物性蛋白質含有食品として、大豆ミート又は納豆等が挙げられる。植物性蛋白質含有食品に含まれる蛋白質含有量は特に制限されないが、3質量%以上50質量%以下であってもよい。より具体的にはその下限は3質量%以上、4質量%以上、5質量%以上、6質量%以上、7質量%以上、8質量%以上、9質量%以上、10質量%以上あってもよく、その上限は50質量%以下、45質量%以下、40質量%以下、35質量%以下、30質量%以下であってもよい。 Vegetable protein-containing foods refer to foods and drinks made from raw materials rich in vegetable protein, and processed beans are particularly suitable. Raw materials include whole grains such as soybeans, peas, mung beans, chickpeas, peanuts, lupines, pigeon peas, nata beans, vine beans, kidney beans, adzuki beans, cowpeas, lentils, fava beans, and locust beans. Its pulverized product can be mentioned, and lees obtained by industrially extracting oils and fats and starch from these can also be used. When the food and drink are these vegetable protein-containing foods, specific vegetable protein-containing foods of the present invention include soybean meat, natto (fermented soybeans), and the like. The protein content contained in the vegetable protein-containing food is not particularly limited, but may be 3% by mass or more and 50% by mass or less. More specifically, the lower limit is 3 mass% or more, 4 mass% or more, 5 mass% or more, 6 mass% or more, 7 mass% or more, 8 mass% or more, 9 mass% or more, 10 mass% or more Well, the upper limit may be 50% by mass or less, 45% by mass or less, 40% by mass or less, 35% by mass or less, or 30% by mass or less.
 また、「喫食に供される飲食品を調製する組成物」としては、特に限定はされないが、例えば、調味料、レトルト食品、飲料を調製するための組成物、スープを調製するための組成物、トッピング材等が挙げられる。これらの中でも、調味料、レトルト食品が好ましく、調味料がより好ましい。 In addition, the "composition for preparing food and drink to be eaten" is not particularly limited, but for example, seasonings, retort foods, compositions for preparing beverages, and compositions for preparing soups. , topping materials, and the like. Among these, seasonings and retort foods are preferred, and seasonings are more preferred.
 調味料としては、例えば、つゆ類(めんつゆ、鍋つゆ等)、たれ類(ゴマだれ等のゴマ含有調味料、焼肉だれ等)、だし類(例えば野菜類のだし、魚介類のだし、肉類のだし)、エキス類(例えば野菜類のエキス、魚介類のエキス、肉類のエキス)、ソース類(パスタソース、ピザソース、ウスターソース、ケチャップソース、オイスターソース、サルサソース、サンバルソース、チリソース、デミグラスソース、ホワイトソース(ベシャメルソース)等)、ドレッシング類(ノンオイルドレッシング、分離ドレッシング、乳化ドレッシング等)、食酢、調味酢(例えば汎用性調味酢、酢の物用調味酢、すし飯用調味酢、酢漬け(例えばピクルス等)用調味液、甘酢等)、米飯用調味料、しょうゆ(日本農林規格(平成16年9月13日農林水産省告示第1703号)に記載されているつけ醤油、かけ醤油及び調理用の用途で利用される醤油、あるいは、上記用途に使用される醤油に類似の調味料を含む)、ぽん酢調味料(ぽん酢、ぽん酢醤油)、納豆用調味料、漬物用調味料、肉用調味料、スパイス含有調味料、チャツネ、マスタード、マヨネーズ等が挙げられる。これらの調味料のなかでも、つゆ類、たれ類、だし類、エキス類、ドレッシング類、ソース類、醤油、ぽん酢調味料が好ましい。 Seasonings include, for example, soups (noodle soup, nabetsuyu, etc.), sauces (sesame-containing seasonings such as sesame sauce, yakiniku sauce, etc.), soup stocks (for example, vegetable stocks, seafood stocks, meat stocks, etc.) Dashi), extracts (e.g. vegetable extracts, seafood extracts, meat extracts), sauces (pasta sauce, pizza sauce, Worcestershire sauce, ketchup sauce, oyster sauce, salsa sauce, sambal sauce, chili sauce, demi-glace sauce, white sauce) (bechamel sauce), etc.), dressings (non-oil dressing, separated dressing, emulsified dressing, etc.), vinegar, seasoning vinegar (e.g. general-purpose seasoning vinegar, seasoning vinegar for vinegared dishes, seasoning vinegar for sushi rice, seasoning for pickles (e.g. pickles, etc.) liquid, sweet vinegar, etc.), seasonings for cooked rice, soy sauce (used for dipping soy sauce, soy sauce for pouring, and cooking purposes described in Japanese Agricultural Standards (Ministry of Agriculture, Forestry and Fisheries Notification No. 1703, September 13, 2004) or seasonings similar to soy sauce used for the above purposes), ponzu seasonings (ponzu, ponzu soy sauce), seasonings for natto, seasonings for pickles, seasonings for meat, seasonings containing spices , chutney, mustard, mayonnaise and the like. Among these seasonings, soups, sauces, soups, extracts, dressings, sauces, soy sauce, and ponzu seasonings are preferred.
 レトルト食品とは、レトルトパウチ又は缶内に調理済み又は半調理済みの食品が詰められ、レトルト加熱(最も一般的には120℃、30~60分の加圧加熱)される食品をいう。ここで、調理済み又は半調理済みの食品としては、例えば、上記した食品そのもの、又は簡単な調理(例えば、食材を加えて加熱調理するなど)により上記した食品を得ることができるもの等が挙げられる。 Retort food refers to food that is packed with cooked or semi-cooked food in a retort pouch or can, and that is retort-heated (most commonly, 120°C, pressurized heating for 30-60 minutes). Here, the cooked or semi-cooked food includes, for example, the above-described food itself, or a food that can be obtained by simple cooking (for example, by adding ingredients and cooking with heat). be done.
 飲料を調製するための組成物としては、例えば飲料の濃縮タイプが挙げられる。これは、水又は適当な飲料(例えば、上記で例示された飲料)で希釈してから、飲用に供される。推奨される希釈倍率は、例えば1.1~50倍、好ましくは2~20倍、より好ましくは3~12倍、さらに好ましくは4~8倍である。 Compositions for preparing beverages include, for example, concentrated types of beverages. This is diluted with water or a suitable beverage (eg, the beverages exemplified above) prior to consumption. A recommended dilution ratio is, for example, 1.1 to 50 times, preferably 2 to 20 times, more preferably 3 to 12 times, still more preferably 4 to 8 times.
 スープを調製するための組成物とは、野菜類、肉類、魚介類、乳製品、米飯類、又は麺類と共に加熱したり、上記食材を調理後合わせてスープ等の食品に調製したりするスープの素が挙げられる。スープの素は、そのまま使用することができる形態(ストレートタイプ)のもの、又は、冷水、温水、だしその他の液状物と混合(希釈)して使用する濃縮タイプ、あるいは、粉末タイプが挙げられる。 A composition for preparing a soup is a soup that is heated with vegetables, meat, seafood, dairy products, rice, or noodles, or that is prepared into a food such as soup by combining the above ingredients after cooking. Elements are mentioned. The soup base may be in a form (straight type) that can be used as it is, a concentrated type that is used after being mixed (diluted) with cold water, hot water, broth or other liquids, or a powder type.
 トッピング材としては、例えば、米飯食品用トッピング材、麺用トッピング材、パン食用トッピング材、サラダ用トッピング材が挙げられる。具体的には、米飯食品用トッピング材としては、ふりかけ、おむすびの素、お粥の素、お茶漬けの素、雑炊の素、混ぜご飯の素、又は炊き込みご飯の素が挙げられる。 Examples of topping materials include rice topping materials, noodle topping materials, bread topping materials, and salad topping materials. Specifically, examples of topping materials for cooked rice foods include furikake, rice ball mix, rice porridge mix, ochazuke mix, rice porridge mix, mixed rice mix, and cooked rice mix.
 本発明の対象となる飲食品としては、上記のような一般的な飲食品のほか、医薬品以外で健康の維持や増進を目的として摂取できる食品、例えば、健康食品、機能性食品、保健機能食品、又は特別用途食品を含む意味で用いられる。健康食品には、栄養補助食品、健康補助食品、サプリメント等の名称で提供される食品を含む。保健機能食品は食品衛生法又は健康増進法により定義され、特定の保健の効果や栄養成分の機能、疾病リスクの低減などを表示できる、特定保健用食品及び栄養機能食品、ならびに科学的根拠に基づいた機能性について消費者庁長官に届け出た内容を表示できる機能性表示食品が含まれる。また特別用途食品には、特定の対象者や特定の疾患を有する患者に適する旨を表示する病者用食品、高齢者用食品、乳児用食品、妊産婦用食品等が含まれる。ここで、飲食品に付される特定の保健の効果や栄養成分の機能等の表示は、製品の容器、包装、説明書、添付文書などの表示物、製品のチラシやパンフレット、新聞や雑誌等の製品の広告などにすることができる。 The food and drink to be the subject of the present invention include, in addition to the above general food and drink, foods other than pharmaceuticals that can be ingested for the purpose of maintaining and improving health, such as health foods, functional foods, and foods with health claims. , or is used in the sense of including food for special uses. Health foods include foods provided under the names of nutritional supplements, health supplements, supplements, and the like. Foods with health claims are defined by the Food Sanitation Law or the Health Promotion Law. This includes foods with function claims that can display the content notified to the Commissioner of the Consumer Affairs Agency regarding the functionality of the food. Foods for special dietary uses include foods for the sick, foods for the elderly, foods for infants, foods for pregnant women, etc., which are labeled as being suitable for specific subjects or patients with specific diseases. Here, indications such as specific health effects and functions of nutritional ingredients attached to food and drink are indicated on product containers, packaging, instructions, and attached documents, product flyers and pamphlets, newspapers and magazines, etc. can be advertisements for products, etc.
 飲食品の形態は、食用に適した形態、例えば、固形状、液状、顆粒状、粒状、粉末状、カプセル状、クリーム状、ペースト状のいずれであってもよい。特に、上記の健康食品等の場合の形状としては、例えば、液状(シロップ状、乳状、懸濁状を含む)、タブレット状、丸状、カプセル状、粉末状、顆粒状、細粒状、トローチ状等が好ましい。 The form of food and drink may be any form suitable for eating, such as solid, liquid, granule, grain, powder, capsule, cream, or paste. In particular, the shape of the above-mentioned health food and the like includes liquid (including syrup, milk, and suspension), tablet, round, capsule, powder, granule, fine grain, and lozenge. etc. are preferred.
 本発明の飲食品は、飲食品の種類に応じて、他の原料や添加剤を含有することができる。他の原料や添加剤としては、油脂類、酒類、甘味料、酸味料、香辛料又は香辛料抽出物、粘度調整剤、色素類、安定剤、酸化防止剤、pH調節剤等が挙げられる。これら他の原料や添加剤の組み合わせ及び含有量は、特に限定はされず、飲食品の種類に応じて適宜設定することができる。 The food and drink of the present invention can contain other raw materials and additives depending on the type of food and drink. Other raw materials and additives include oils and fats, alcoholic beverages, sweeteners, acidulants, spices or spice extracts, viscosity modifiers, pigments, stabilizers, antioxidants, pH adjusters and the like. The combination and content of these other raw materials and additives are not particularly limited, and can be appropriately set according to the type of food or drink.
2.飲食品の呈味と香りの向上方法
 本発明によればまた、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を、呈味成分を含む飲食品又はその原料に添加することを特徴とする、飲食品の呈味と香りの向上方法が提供される。
2. Method for Improving Taste and Aroma of Foods and Beverages According to the present invention, one or more selected from terpinyl acetate, apigenin, and luteolin are added to food and beverages containing taste components or raw materials thereof. A method for improving the taste and aroma of food and drink characterized by the above is provided.
 酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を、呈味成分を含む飲食品又はその原料に添加するタイミングは、特に限定されない。該タイミングとしては、例えば飲食品の製造時、飲食品の製造後、喫食前等が挙げられる。 The timing of adding one or more selected from terpinyl acetate, apigenin, and luteolin to a food or drink containing taste components or its ingredients is not particularly limited. The timing includes, for example, during production of the food and drink, after production of the food and drink, and before eating.
3.呈味と香りが向上した飲食品の製造方法
 本発明によればまた、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を、呈味成分を含む飲食品又はその原料に添加することを特徴とする、呈味と香りが向上した飲食品の製造方法が提供される。
3. Method for Producing Food and Beverage with Improved Taste and Aroma According to the present invention, one or more selected from terpinyl acetate, apigenin, and luteolin are added to food and drink containing taste-imparting components or raw materials thereof. A method for producing food and drink with improved taste and aroma is provided.
 本発明の飲食品の製造方法の好ましい一実施態様は、下記の段階(i)~(iii)を含む方法で植物素材抽出物を調製することを含む。
 (i) 酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材を抽出溶媒に添加する段階
 (ii) 植物素材から抽出溶媒にて成分抽出し、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材抽出物を得る工程
 (iii) (ii)で得られた植物素材抽出物のpHを相対的に酸性側に調整する段階
A preferred embodiment of the method for producing food or drink of the present invention comprises preparing a plant material extract by a method including the following steps (i) to (iii).
(i) A step of adding a plant material containing one or more selected from terpinyl acetate, apigenin, and luteolin to an extraction solvent (ii) Extracting components from the plant material with an extraction solvent, and a step of obtaining a plant material extract containing one or more selected from luteolin (iii) a step of adjusting the pH of the plant material extract obtained in (ii) to be relatively acidic.
 本発明の飲食品の製造方法の好ましい別の一実施態様は、前記段階(ii)の後、植物素材抽出物のpHを6.5以上12.0以下に調整する段階をさらに含む。 Another preferred embodiment of the method for producing a food or drink of the present invention further comprises a step of adjusting the pH of the plant material extract to 6.5 or more and 12.0 or less after the step (ii).
 段階(i)では、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材を抽出溶媒に添加する。植物素材及び抽出溶媒の種類は前記のとおりである。 In step (i), a plant material containing one or more selected from terpinyl acetate, apigenin, and luteolin is added to the extraction solvent. The types of plant material and extraction solvent are as described above.
 段階(ii)では、植物素材から抽出溶媒にて成分抽出し、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材抽出物を得る。このとき、抽出溶媒のpHを室温において中性からアルカリ性、例えば、6.5以上11.0以下に調整することが好ましい。より具体的には、抽出溶媒のpHは6.5以上、7.0以上、7.5以上、8.0以上、9.0以上、その上限は特に制限されないが、通常11.0以下、10.5以下、10.0以下とすればよい。 In step (ii), components are extracted from the plant material with an extraction solvent to obtain a plant material extract containing one or more selected from terpinyl acetate, apigenin, and luteolin. At this time, it is preferable to adjust the pH of the extraction solvent from neutral to alkaline at room temperature, for example, from 6.5 to 11.0. More specifically, the pH of the extraction solvent is 6.5 or higher, 7.0 or higher, 7.5 or higher, 8.0 or higher, or 9.0 or higher. 10.5 or less, 10.0 or less.
 また、前記段階(ii)の後、植物素材抽出物のpHを6.5以上12.0以下に調整する段階を含む場合には、植物素材抽出物のpHを室温において中性からアルカリ性、例えば、6.5以上12.0以下に調整することが好ましい。より具体的には、植物素材抽出物のpHは6.5以上、7.0以上、7.5以上、8.0以上、9.0以上、その上限は特に制限されないが、通常12.0以下、11.5以下、11.0以下とすればよい。 In addition, after the step (ii), when the step of adjusting the pH of the plant material extract to 6.5 or more and 12.0 or less is included, the pH of the plant material extract is adjusted to neutral to alkaline at room temperature, for example , 6.5 or more and 12.0 or less. More specifically, the pH of the plant material extract is 6.5 or higher, 7.0 or higher, 7.5 or higher, 8.0 or higher, or 9.0 or higher. Below, it may be 11.5 or less and 11.0 or less.
 植物素材抽出物のpHの調整の他、外部から酢酸テルピニル、アピゲニン、又はルテオリン試薬等の少なくとも1種を添加する場合等には、これらの成分を含む溶液に対してpHの調整を行っても良い。pHの調整は、植物素材抽出物や上記溶液にpH調整剤(例えば水酸化ナトリウム、水酸化カリウム、炭酸ナトリウム、炭酸カリウム、炭酸カルシウム、炭酸水素ナトリウム、グルコン酸カリウム、乳酸、クエン酸、酒石酸、リンゴ酸及び酢酸等)を溶解させることによって行うことができる。上記pH調整剤のなかでも、特に炭酸塩である炭酸ナトリウム、炭酸カリウム又は炭酸カルシウムを用いることが好ましい。 In addition to adjusting the pH of the plant material extract, when adding at least one of terpinyl acetate, apigenin, or luteolin reagent from the outside, the pH of the solution containing these components may be adjusted. good. The pH is adjusted by adding a pH adjusting agent (e.g., sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, calcium carbonate, sodium hydrogen carbonate, potassium gluconate, lactic acid, citric acid, tartaric acid, malic acid, acetic acid, etc.). Among the above pH adjusters, it is particularly preferable to use carbonates such as sodium carbonate, potassium carbonate, or calcium carbonate.
 段階(iii)では、上記抽出により得られた植物素材抽出物のpHを相対的に酸性側に調整する。本段階の技術的意義は、段階(ii)の抽出段階後に得られた植物素材抽出物(単に「段階(ii)抽出物」と称する場合がある)の室温でのpHを、抽出開始段階よりも相対的に酸性側に調整することで、本発明の飲食品の成分(酢酸テルピニル、アピゲニン、又はルテオリン)を固定化することにある。具体的には、段階(ii)抽出物(すなわち段階(ii)の抽出段階後に得られた抽出物)のpHと、段階(i)で用いた抽出溶媒のpHとの低下差分が0.1以上10.0以下となるようにpHを調整することが好ましい。より具体的には、その下限は0.1以上、0.2以上、0.3以上、0.4以上、0.5以上、0.6以上0.7以上、0.8以上、0.9以上1.0以上であればよく、その上限は10.0以下、9.0以下、8.0以下、7.0以下、6.0以下、5.0以下、4.0以下3.0以下であればよい。 In step (iii), the pH of the plant material extract obtained by the above extraction is adjusted to be relatively acidic. The technical significance of this step is that the pH at room temperature of the plant material extract obtained after the extraction step of step (ii) (sometimes simply referred to as “step (ii) extract”) is is also adjusted to be relatively acidic to immobilize the ingredients (terpinyl acetate, apigenin, or luteolin) of the food and drink of the present invention. Specifically, the difference in pH drop between the pH of the step (ii) extract (i.e. the extract obtained after the extraction step of step (ii)) and the pH of the extraction solvent used in step (i) is 0.1. It is preferable to adjust the pH to 10.0 or less. More specifically, the lower limit is 0.1 or more, 0.2 or more, 0.3 or more, 0.4 or more, 0.5 or more, 0.6 or more and 0.7 or more, 0.8 or more, 0.8 or more. 9 or more and 1.0 or more, and the upper limits are 10.0 or less, 9.0 or less, 8.0 or less, 7.0 or less, 6.0 or less, 5.0 or less, 4.0 or less3. 0 or less is acceptable.
 また、抽出に用いる溶媒を任意で加熱しても良い。加熱条件は特に限定されないが、加熱温度は例えば50℃以上105℃以下の範囲とすることができ、処理時間は例えば30秒以上30分間未満の範囲とすることができる。より具体的に、加熱温度の下限は、例えば50℃以上、又は55℃以上、又は60℃以上、又は65℃以上、又は70℃以上、又は75℃以上、又は80℃以上、又は85℃以上、又は90℃以上、又は95℃以上、その上限は通常105℃以下、又は100℃以下とすることができ、加熱時間の下限は、例えば30秒以上、又は1分間以上、又は2分間以上、その上限は例えば30分間未満、25分間未満、20分間未満、15分間未満、又は10分間未満、又は5分間未満とすることができる。一般的に加熱温度と加熱時間とは略相互依存の関係にもあり、加熱温度を高くするほど加熱時間は概ね短くて済む一方で、加熱時間を長くするほど加熱温度は概ね低くて済む傾向がある。よって、斯かる加熱温度及び加熱時間の関係を考慮し、それぞれ適切な範囲となるように設定すればよい。具体的には70℃以上105℃以下の範囲で25分間未満の加熱抽出を行ってもよく、80℃以上105℃以下の範囲で20分間未満の加熱抽出を行ってもよく、85℃以上105℃以下の範囲で15分間未満の加熱抽出を行ってもよい。また、植物素材から目的とする成分を効率よく抽出しその他の植物素材由来の成分が可能な限り混入させないために、本工程においては静置、或いは攪拌することが望ましく、ホモジナイゼーション等破砕を行わないことが望ましい。 In addition, the solvent used for extraction may optionally be heated. The heating conditions are not particularly limited, but the heating temperature can be in the range of, for example, 50° C. or more and 105° C. or less, and the treatment time can be in the range of, for example, 30 seconds or more and less than 30 minutes. More specifically, the lower limit of the heating temperature is, for example, 50°C or higher, or 55°C or higher, or 60°C or higher, or 65°C or higher, or 70°C or higher, or 75°C or higher, or 80°C or higher, or 85°C or higher. , or 90 ° C. or higher, or 95 ° C. or higher, the upper limit is usually 105 ° C. or lower, or 100 ° C. or lower, and the lower limit of the heating time is, for example, 30 seconds or more, or 1 minute or more, or 2 minutes or more, The upper limit can be, for example, less than 30 minutes, less than 25 minutes, less than 20 minutes, less than 15 minutes, or less than 10 minutes, or less than 5 minutes. In general, the heating temperature and heating time are almost interdependent, and while the higher the heating temperature, the shorter the heating time, the longer the heating time, the lower the heating temperature. be. Therefore, the relationship between the heating temperature and the heating time should be taken into account, and the respective ranges should be set appropriately. Specifically, heat extraction may be performed in the range of 70 ° C. or higher and 105 ° C. or lower for less than 25 minutes, or heat extraction may be performed in the range of 80 ° C. or higher and 105 ° C. or lower for less than 20 minutes. You may heat extraction for less than 15 minutes in the range below °C. In addition, in order to efficiently extract the target component from the plant material and avoid contamination with other plant material-derived components as much as possible, it is desirable to leave the plant to stand or stir in this step, and crushing such as homogenization is not required. It is desirable not to do so.
 また、段階(iii)において相対的に酸性側に調整された後の抽出物のpHが所定の範囲に調整されていても良い。具体的には、段階(iii)において相対的に酸性側に調整された後の抽出物(植物素材抽出物)のpHが室温において1.5以上7.0未満となるように調整することが好ましい。より具体的には、その下限は1.5以上、2.0以上、2.5以上、3.0以上であればよく、その上限は特に制限されないが、通常7.0未満、6.5未満、6.0未満、5.5未満、5.0未満、4.6未満、4.0未満であればよい。 In addition, the pH of the extract after being adjusted to be relatively acidic in step (iii) may be adjusted within a predetermined range. Specifically, the pH of the extract (plant material extract) adjusted to be relatively acidic in step (iii) can be adjusted to be 1.5 or more and less than 7.0 at room temperature. preferable. More specifically, the lower limit may be 1.5 or more, 2.0 or more, 2.5 or more, or 3.0 or more, and the upper limit is not particularly limited, but usually less than 7.0 and 6.5. less than, less than 6.0, less than 5.5, less than 5.0, less than 4.6, less than 4.0.
 以下、実施例により本発明をさらに具体的に説明する。但し、本発明はこれらに限定されるものではない。 Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these.
(参考例)
 下記の試験例において、評価サンプル中の酢酸テルピニル、アピゲニン、ルテオリンの含有量、グルタミン酸の含有量、イノシン酸の含有量の測定は、以下の通り行った。
(1)酢酸テルピニルの定量方法
 まず、サンプルを、以下の条件下で固相マイクロ抽出法(SPME)に供することにより、サンプル中の酢酸テルピニルを分離濃縮した。
(Reference example)
In the following test examples, the contents of terpinyl acetate, apigenin, luteolin, glutamic acid, and inosinic acid in evaluation samples were measured as follows.
(1) Terpinyl Acetate Quantification Method First, a sample was subjected to solid-phase microextraction (SPME) under the following conditions to separate and concentrate terpinyl acetate in the sample.
<固相マイクロ抽出条件>
・SPMEファイバー:StableFlex 50/30μm、DVB/Carboxen/PDMS(SUPELCO社製)
・揮発性成分抽出装置:PAL3 RSI120(CTC Analytics社製)
・予備加熱:40℃15分間
・揮発性成分抽出:80℃、20分間
・吸着:10分間
・脱着時間:10分間
<Solid-phase microextraction conditions>
・SPME fiber: StableFlex 50/30 μm, DVB/Carboxen/PDMS (manufactured by SUPELCO)
・Volatile component extraction device: PAL3 RSI120 (manufactured by CTC Analytics)
・Preheating: 40°C for 15 minutes ・Volatile component extraction: 80°C for 20 minutes ・Adsorption: 10 minutes ・Desorption time: 10 minutes
 次に、固相マイクロ抽出法により分離濃縮されたサンプル中の酢酸テルピニルについて、ガスクロマトグラフ法及び質量分析法を用い、以下の条件に従ってガスクロマトグラフ分析を行った。 Next, the terpinyl acetate in the sample separated and concentrated by the solid-phase microextraction method was analyzed by gas chromatography and mass spectrometry under the following conditions.
<ガスクロマトグラフ条件>
・測定機器:Agilent 7890B GC System(Agilent Technologies社製)
・GCカラム:DB-WAX(Agilent Technologies社製)、長さ30m、口径0.25mm、膜厚0.25μm
・キャリア:Heガス、ガス流量1.0mL/分間
・温度条件:40℃で3分間保持→250℃まで10℃/分ずつ昇温→250℃で10分間保持
<Gas chromatograph conditions>
- Measuring instrument: Agilent 7890B GC System (manufactured by Agilent Technologies)
・ GC column: DB-WAX (manufactured by Agilent Technologies), length 30 m, diameter 0.25 mm, film thickness 0.25 μm
・Carrier: He gas, gas flow rate 1.0 mL/min ・Temperature conditions: Hold at 40°C for 3 minutes → Increase temperature to 250°C by 10°C/min → Hold at 250°C for 10 minutes
<質量分析条件>
・測定機器:Agilent 7000C GC/MS Triple Quad(Agilent Technologies社製)
・イオン化方式:EI(イオン化電圧70eV)
<Conditions for mass spectrometry>
- Measuring instrument: Agilent 7000C GC/MS Triple Quad (manufactured by Agilent Technologies)
・Ionization method: EI (ionization voltage 70 eV)
 酢酸テルピニルの確認イオン(m/z=121)に基づいて酢酸テルピニルのピークを特定し、ピーク面積を求めた。得られた酢酸テルピニルのピーク面積から、各サンプルに含まれる酢酸テルピニルの濃度を算出した。 A terpinyl acetate peak was identified based on the confirmation ion (m/z=121) of terpinyl acetate, and the peak area was determined. From the obtained peak area of terpinyl acetate, the concentration of terpinyl acetate contained in each sample was calculated.
(2)アピゲニン、ルテオリン定量方法
 以下の手順で定量用のサンプルを調製した。
標品:
 濃度既知のルテオリン、アピゲニン試薬を希釈し、成分濃度0.00025ppm、0.0005ppm、0.005ppm、0.05ppm、0.5ppmの標品としてLCMSに供試した。
(2) Apigenin, luteolin quantification method Samples for quantification were prepared by the following procedure.
Sample:
Luteolin and apigenin reagents with known concentrations were diluted and tested for LCMS as standards with component concentrations of 0.00025 ppm, 0.0005 ppm, 0.005 ppm, 0.05 ppm and 0.5 ppm.
分析サンプル:
 測定対象試料(例えばパセリ2%抽出物)を4倍容量のメタノールで希釈し、ろ過後LCMSに供試した。
Analysis sample:
A sample to be measured (for example, 2% parsley extract) was diluted with 4 volumes of methanol, filtered, and then subjected to LCMS.
<LCMS条件>
使用機器:
・LC:SHIMADZU / Nexera XS
・MS:Bruker / impact II
・解析ソフト:Bruker / Compass Data Analysis
・カラム:ACQUITY UPLC BEH C18 1.7μm 2.1x50mm Column
<LCMS conditions>
Used equipment:
・LC: Shimadzu / Nexera XS
・ MS: Bruker / impact II
・ Analysis software: Bruker / Compass Data Analysis
・Column: ACQUITY UPLC BEH C18 1.7 μm 2.1×50 mm Column
LC分析条件:
カラム温度:40℃
流速 0.4mL/min 
注入量 10μLアプライ
  移動相A:0.1%ギ酸 水
  移動相B:0.1%ギ酸 アセトニトリル
  グラジエント(移動相Aと移動相Bの割合)
  0-1min  95%A:5%B
  1.5min  90%A:10%B
  4.5min  70%A:30%B
  5.5min  60%A:40%B
  7min    50%A:50%B
  9.5min  30%A:70%B
  9.5-12.5min 100%B
LC analysis conditions:
Column temperature: 40°C
Flow rate 0.4mL/min
Injection volume 10 μL applied Mobile phase A: 0.1% formic acid water Mobile phase B: 0.1% formic acid acetonitrile Gradient (percentage of mobile phase A and mobile phase B)
0-1 min 95% A: 5% B
1.5 min 90% A: 10% B
4.5 min 70% A: 30% B
5.5 min 60% A: 40% B
7 min 50% A: 50% B
9.5 min 30% A: 70% B
9.5-12.5min 100%B
MS条件:イオン源温度:200°C
イオン化方式:ESI(イオン化電圧5.0eV)
MSスキャン:m/z 50~1,500
MS conditions: ion source temperature: 200°C
Ionization method: ESI (ionization voltage 5.0 eV)
MS scan: m/z 50-1,500
 各試料について、ポジティブモードでH+付加イオンについて分析し、定量イオン(ルテオリン定量イオンm/z 287.0484±0.020、アピゲニン定量イオン m/z 271.0537±0.020)に基づいて各試料中の成分ピークを特定し、ピーク面積を求めた。得られた各成分のピーク面積から、溶媒での希釈率を考慮して、各試料に含まれる各成分の濃度を算出した。 Each sample was analyzed for H+ adduct ions in positive mode, and the The peak area of the peak was determined by identifying the peak of the component inside. From the obtained peak area of each component, the concentration of each component contained in each sample was calculated in consideration of the dilution ratio with the solvent.
(3)グルタミン酸の含有量の定量方法
 「日本食品標準成分表2015年版(七訂)分析マニュアル」のアミノ酸分析法を用い、以下の条件に従って、グルタミン酸の含有量を測定した。
・測定機器:アミノ酸自動分析計(日本電子製、機種JLC-500/V2)
・カラム:生体分析パックドカラム、内径4.6mm、長さ60mm,ステンレス製
・移動相:生体液分析法用緩衝液 PFセット KANTO(関東化学社製)
・反応液:日立用ニンヒドリン発色溶液キット(富士フィルム和光純薬社製)
・波長:570nm
(3) Method for quantifying glutamic acid content The content of glutamic acid was measured according to the following conditions using the amino acid analysis method of the “Japanese Food Standard Composition Tables 2015 Edition (7th Edition) Analysis Manual”.
・Measurement equipment: Amino acid automatic analyzer (manufactured by JEOL, model JLC-500/V2)
・Column: Bioanalytical packed column, inner diameter 4.6 mm, length 60 mm, made of stainless steel ・Mobile phase: Buffer solution for biological fluid analysis PF set KANTO (manufactured by Kanto Chemical Co., Ltd.)
・Reaction solution: Ninhydrin coloring solution kit for Hitachi (manufactured by Fuji Film Wako Pure Chemical Industries, Ltd.)
・Wavelength: 570 nm
(4)イノシン酸の含有量の定量方法
 サンプルは、超純水で希釈し、以下の条件に従って、高速液体クロマトグラフィー(HPLC)を用い、イノシン酸のピーク面積を分析した。また、超純水で希釈した5mg/100mLのイノシン酸を、標準サンプルとして同様に分析し、外部標準法により各サンプルのイノシン酸の含有量を算出した。
(4) Method for quantifying inosinic acid content The sample was diluted with ultrapure water, and the peak area of inosinic acid was analyzed using high performance liquid chromatography (HPLC) under the following conditions. In addition, 5 mg/100 mL of inosinic acid diluted with ultrapure water was similarly analyzed as a standard sample, and the content of inosinic acid in each sample was calculated by the external standard method.
<高速液体クロマトグラフィー(HPLC)条件>
・測定機器:高速液体クロマトグラフィー(島津製作所社製)
・移動相:リン酸二水素カリウム 6.8g/L(pH3.5)、1mL/min
・カラム:YMC-Pack Polyamine II(ワイエムシィ社製)
・カラム温度:35℃
・検出:UV260nm
<High performance liquid chromatography (HPLC) conditions>
・Measurement equipment: high-performance liquid chromatography (manufactured by Shimadzu Corporation)
- Mobile phase: potassium dihydrogen phosphate 6.8 g / L (pH 3.5), 1 mL / min
・Column: YMC-Pack Polyamine II (manufactured by YMC)
・Column temperature: 35°C
・Detection: UV260nm
(試験例1)酢酸テルピニルの含有量の検討(つゆによる評価)
(1)試験品の調製
 酢酸テルピニル(井上香料社製、Specification:100%)を1質量%になるよう水に溶解した。この溶液を更に水にて希釈し、表1の試験品1~7に記載の濃度の酢酸テルピニル溶液を調製した。
(Test Example 1) Examination of terpinyl acetate content (evaluation by soup)
(1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare terpinyl acetate solutions having the concentrations shown in Table 1 for test products 1 to 7.
 また、乾燥セロリ(リーフ社製)を70℃の熱湯に対し2質量%となるように加え、65℃で15分間静置した後、ろ紙(No.2)にてろ過し、室温まで放置冷却して抽出液を得た(酢酸テルピニル含有量5.58×10-3ppm)。この溶液を更に水にて希釈し、表1の試験品8、9に記載の濃度の酢酸テルピニル溶液を調製した。 In addition, dry celery (manufactured by Leaf Co., Ltd.) was added to hot water at 70°C so as to be 2% by mass, allowed to stand at 65°C for 15 minutes, filtered with filter paper (No. 2), and left to cool to room temperature. to obtain an extract (terpinyl acetate content: 5.58×10 −3 ppm). This solution was further diluted with water to prepare terpinyl acetate solutions having concentrations shown in Test Products 8 and 9 in Table 1.
 表1に示す配合量(質量%)に従い、鰹節だし、濃口醤油、砂糖、本みりん、食塩、酢酸テルピニル溶液、水を混合し、試験品1~9のつゆを調製した。また、鰹節だし、濃口醤油、砂糖、本みりん、食塩の配合量は同じで、酢酸テルピニル溶液を添加せず、水66.5質量%としたつゆをコントロールとした。 Dried bonito stock, dark soy sauce, sugar, mirin, salt, terpinyl acetate solution, and water were mixed according to the blending amounts (mass%) shown in Table 1 to prepare test products 1 to 9. In addition, the control was a soup containing 66.5% by mass of water without adding the terpinyl acetate solution, with the same blending amounts of dried bonito stock, dark soy sauce, sugar, mirin, and salt.
 調製した各試験品における酢酸テルピニルの含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した各試験品をそのまま用いる常温サンプル、及び(1)で調製した各試験品をレトルト用パウチに充填、シールした後70℃5分加温した加温サンプルそれぞれについて、下記分析型官能評価専門パネラー4名により、官能評価を行った。
(2) Sensory evaluation test A room temperature sample using each test item prepared in (1) as it is, and a retort pouch filled with each test item prepared in (1), sealed and then heated at 70 ° C. for 5 minutes. Sensory evaluation was performed on each of the samples by four panelists specializing in the following analysis-type sensory evaluation.
(分析型官能評価専門パネラー)
 分析型官能評価専門パネラーは、味や香りに関する判定能力が、下記の識別試験(I)及び識別試験(II)により担保された専門パネラーである。
 識別試験(I):味質識別試験
 五味(甘味:砂糖の味、酸味:酒石酸の味、うま味:グルタミン酸ナトリウムの味、塩味:塩化ナトリウムの味、苦味:カフェインの味)について、各成分の閾値に近い濃度の水溶液を各1つずつ作製し、これに蒸留水2つを加えた計7つのサンプルから、それぞれの味のサンプルを正確に識別する味質識別試験。
 識別試験(II):濃度がわずかに異なる5種類の食塩水溶液、酢酸水溶液の濃度差を正確に識別する濃度差識別試験。
(Analytical sensory evaluation expert panelist)
The analysis-type sensory evaluation expert panelists are expert panelists whose ability to judge tastes and aromas is guaranteed by the following identification test (I) and identification test (II).
Identification test (I): Taste identification test Five tastes (sweetness: sugar taste, sourness: tartaric acid taste, umami: sodium glutamate taste, salty taste: sodium chloride taste, bitterness: caffeine taste) A taste discrimination test in which each taste sample is accurately identified from a total of 7 samples in which 2 distilled water is added to an aqueous solution with a concentration close to the threshold value.
Discrimination test (II): Concentration difference discrimination test for accurately discriminating the difference in concentration between five types of saline solutions and acetic acid solutions having slightly different concentrations.
(評価方法)
 官能評価は、(i)サンプルの提示、(ii)官能評価項目のすり合わせ、(iii)試し評価・キャリブレーション、(iv)本評価の順に行った。
(i)サンプルの提示
 官能評価におけるパネラーのバイアス(偏り)を排除し、評価の精度を高めるために、サンプル提供を次の通りに設定した。100mL用ペットボトルに充填密閉したサンプルを常温(25℃)にし、評価毎に上記容器から20mLプラスチックカップに計量スプーンで小さじ1杯(約5mL)程度移し、各パネラーに提示した。その際、サンプルの試験区番号や内容物の情報はパネラーに知らせず、各試験区のサンプルをランダムに提示した。
(Evaluation method)
The sensory evaluation was performed in the order of (i) sample presentation, (ii) adjustment of sensory evaluation items, (iii) trial evaluation/calibration, and (iv) final evaluation.
(i) Presentation of samples Sample presentation was set as follows in order to eliminate the panelist's bias in the sensory evaluation and improve the accuracy of the evaluation. A sample filled and sealed in a 100 mL PET bottle was brought to room temperature (25° C.), and for each evaluation, about 1 teaspoon (about 5 mL) was transferred from the container to a 20 mL plastic cup with a measuring spoon and presented to each panelist. At that time, the samples from each test plot were presented at random without informing the panelists of the test plot numbers and contents of the samples.
(ii)官能評価項目のすり合わせ
 評価を実施するにあたり、パネラー全体で討議し、各評価項目の特性に対してすり合わせを行って、各パネラーが共通認識を持つようにした。総合評価についても、官能評価の結果をもとに基準化できるように、パネラー全体で事前に協議した上で設定した。
(ii) Reconciliation of sensory evaluation items In carrying out the evaluation, the panelists as a whole discussed and reconciled the characteristics of each evaluation item so that each panelist had a common understanding. Comprehensive evaluation was also set after prior discussion among all panelists so that it could be standardized based on the sensory evaluation results.
(iii)試し評価・キャリブレーション
 いくつかの酢酸テルピニルの異なるサンプルを用いて、各評価項目について評価基準の訓練を行った。訓練に際しては、パネラー自身の評価結果を伝えることで、繰り返し評価における再現性を確認させた。
(iii) Trial Evaluation/Calibration Several different samples of terpinyl acetate were used to train the evaluation criteria for each evaluation item. During training, the reproducibility of repeated evaluations was confirmed by telling the panelists their own evaluation results.
(iv)本評価
 上記の訓練により各パネラーの評価基準の妥当性を担保した後、試験品及びコントロールを用いて官能評価を行った。具体的には、サンプルを100mLペットボトルに充填密閉し、上記容器から20mLプラスチックカップに評価毎に計量スプーンで小さじ1杯(約5mL)分程度液を口に含んだ瞬間、及びその後飲み込むまでの呈味、及び口腔内から鼻腔へと抜ける際の香気を評価した。また、サンプルを口に含んだ後は、蒸留水を口に含み、口内における風味を十分に消した後、次のサンプルの評価を行った。
(iv) Main Evaluation After ensuring the adequacy of each panelist's evaluation criteria through the training described above, a sensory evaluation was performed using test products and controls. Specifically, the sample is filled and sealed in a 100 mL plastic bottle, and the container is placed in a 20 mL plastic cup for each evaluation. The taste and the aroma when passing from the oral cavity to the nasal cavity were evaluated. In addition, after holding the sample in the mouth, the sample was held in the mouth with distilled water to sufficiently eliminate the flavor in the mouth, and then the next sample was evaluated.
(評価基準)
 官能評価は、上記の分析型官能評価専門パネラー4名にて、下記の評価基準により行った。評価項目は、つゆの「風味のインパクト」、「呈味の広がり」、「総合評価(全体の美味しさ)」とした。コントロールの評価を3点とし、各評価項目について下記の5段階で評価した。評価点の算出は、4名の評価を加重平均した。5点評価の3.5点以上を合格とし、4点以上良好な効果があるものとし、4.5点以上をより良好な効果があるものとし、5点が最も良好な効果があるものとした。また、各評価項目の評価点で1項目でも評価点が3点未満となったものは不合格とした。
(Evaluation criteria)
The sensory evaluation was performed by four panelists specializing in analytical sensory evaluation according to the following evaluation criteria. The evaluation items were "flavor impact", "flavor spread", and "comprehensive evaluation (overall deliciousness)" of the soup. The control was evaluated as 3 points, and each evaluation item was evaluated in the following 5 stages. Calculation of evaluation points was a weighted average of evaluations by four persons. A 5-point evaluation of 3.5 points or more is acceptable, 4 points or more indicates a good effect, 4.5 points or more indicates a better effect, and 5 points indicates the best effect. bottom. In addition, when the evaluation score for each evaluation item was less than 3 even for one item, the evaluation score was disqualified.
<風味のインパクト>
 5点:コントロールと比べ、つゆのだし感と醤油の香りを口に入れた瞬間に強く感じられた。
 4点:コントロールと比べ、つゆのだし感と醤油の香りを口に入れた瞬間にやや強く感じられた。
 3点:コントロールと比べ、つゆのだし感と醤油の香りを口に入れた瞬間に、同程度に感じられた。
 2点:コントロールと比べ、つゆのだし感と醤油の香りを口に入れた瞬間に、やや弱く感じられた。
 1点:コントロールと比べ、つゆのだし感と醤油の香りを口に入れた瞬間に、弱く感じられた。
<Impact of Flavor>
5 points: Compared to the control, the taste of soup stock and the aroma of soy sauce were strongly felt at the moment of putting in the mouth.
4 points: Compared to the control, the taste of soup stock and the aroma of soy sauce were slightly stronger at the moment of putting in the mouth.
3 points: Compared to the control, the taste of soup stock and the aroma of soy sauce were felt at the same level at the moment of putting in the mouth.
2 points: Compared to the control, the taste of soup stock and the aroma of soy sauce were felt slightly weaker at the moment of putting in the mouth.
1 point: Compared to the control, the taste of soup stock and the aroma of soy sauce were felt weaker at the moment of putting in the mouth.
<呈味の広がり>
 5点:コントロールと比べ、つゆ本来のうま味が口全体に広がった。
 4点:コントロールと比べ、つゆ本来のうま味が口に広がった。
 3点:コントロールと比べ、つゆ本来のうま味が同程度であった。
 2点:コントロールと比べ、つゆ本来のうま味がやや弱かった。
 1点:コントロールと比べ、つゆ本来のうま味が弱かった。
<Expansion of taste>
5 points: The umami inherent in the soup spread throughout the mouth compared to the control.
4 points: Compared to the control, the umami inherent in the soup spread in the mouth.
3 points: Compared to the control, the umami inherent in the soup was at the same level.
2 points: The umami inherent in the soup was slightly weaker than the control.
1 point: The umami inherent in the soup was weaker than the control.
<総合評価(全体の美味しさ)>
 5点:コントロールと比べ、全体的につゆの風味がかなり向上した。
 4点:コントロールと比べ、全体的につゆの風味が向上した。
 3点:コントロールと比べ、全体的につゆの風味が同程度であった。
 2点:コントロールと比べ、全体的につゆの風味がやや弱かった。
 1点:コントロールと比べ、全体的につゆの風味が弱かった。
<Comprehensive evaluation (overall taste)>
5 points: Compared to the control, the flavor of the soup was considerably improved as a whole.
4 points: The flavor of the soup was improved as a whole compared to the control.
3 points: Compared to the control, the flavor of the soup was almost the same as that of the control.
2 points: The soup flavor was slightly weak overall compared to the control.
1 point: Overall flavor of soup was weak compared to control.
 各試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)の各含有量(ppm)、(A)/(B)、(A)/(C)、及び官能評価結果を表1に示す。 Each content (ppm) of terpinyl acetate (A), glutamic acid (B), inosinic acid (C), (A) / (B), (A) / (C), and functional Table 1 shows the evaluation results.
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
 表1に示されるように、常温及び加温のいずれの試験品についても、酢酸テルピニルの含有量が0.00000001~10ppmの範囲において、風味のインパクト、呈味の広がり、全体の美味しさに優れていた(試験品2~9)。一方、酢酸テルピニルの含有量が100ppmでは、風味のインパクト、呈味の広がり、全体の美味しさが低下した(試験品1)。 As shown in Table 1, for both room temperature and heated test products, when the terpinyl acetate content is in the range of 0.00000001 to 10 ppm, the impact of flavor, the spread of taste, and the overall deliciousness are excellent. (test products 2 to 9). On the other hand, when the terpinyl acetate content was 100 ppm, the flavor impact, the spread of the taste, and the overall deliciousness decreased (test product 1).
(試験例2)酢酸テルピニル含有量の検討(ドレッシングによる評価)
(1)試験品の調製
 酢酸テルピニル(井上香料社製、Specification:100%)を1質量%になるよう水に溶解した。この溶液を更に水にて希釈し、表2の試験品10~15に記載の濃度の酢酸テルピニル溶液を調製した。
(Test Example 2) Examination of terpinyl acetate content (evaluation by dressing)
(1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare terpinyl acetate solutions having the concentrations shown in Table 2, Test Products 10 to 15.
 ノンオイルドレッシング(Mizkan社製、コクとうまみのノンオイルたっぷり野菜)99質量%に上記酢酸テルピニル溶液1質量%をそれぞれ混合し、試験品10~15のドレッシングを調製した。また、酢酸テルピニル溶液を添加せず、ドレッシング99質量%に水1質量%を混合したものをコントロールとした。 99% by mass of non-oil dressing (manufactured by Mizkan, vegetables rich in richness and umami with no oil) was mixed with 1% by mass of the above terpinyl acetate solution to prepare dressings of test products 10 to 15. A control was prepared by mixing 99% by mass of dressing with 1% by mass of water without adding the terpinyl acetate solution.
 調製した各試験品における酢酸テルピニルの含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した各試験品について、ドレッシングの「風味のインパクト」、「呈味の広がり」、「雑味」、「総合評価(全体の美味しさ)」の各項目の官能評価を、下記の評価基準で行う以外は、試験例1と同様の方法にて行った。
(2) Sensory evaluation test For each test product prepared in (1), each item of dressing “flavor impact”, “taste spread”, “miscellaneous taste”, and “overall evaluation (overall deliciousness)” Sensory evaluation was performed in the same manner as in Test Example 1, except that the following evaluation criteria were used.
<風味のインパクト>
 5点:コントロールと比べ、ドレッシングの野菜由来のうま味とフレッシュな香りが、口に入れた瞬間に強く引き立った。
 4点:コントロールと比べ、ドレッシングの野菜由来のうま味とフレッシュな香りが、口に入れた瞬間にやや強く引き立った。
 3点:コントロールと比べ、ドレッシングの野菜由来のうま味とフレッシュな香りが、口に入れた瞬間に同程度に引き立った。
 2点:コントロールと比べ、ドレッシングの野菜由来のうま味とフレッシュな香りが、口に入れた瞬間にやや弱まった。
 1点:コントロールと比べ、ドレッシングの野菜由来のうま味とフレッシュな香りが、口に入れた瞬間に弱まった。
<Impact of Flavor>
5 points: Compared to the control, the vegetable-derived umami and fresh aroma of the dressing were strongly enhanced the moment it was put in the mouth.
4 points: Compared to the control, the vegetable-derived umami and fresh aroma of the dressing were slightly stronger at the moment of putting it in the mouth.
3 points: Compared to the control, the vegetable-derived umami and fresh aroma of the dressing stood out to the same degree the moment it was put in the mouth.
2 points: Compared to the control, the vegetable-derived umami and fresh aroma of the dressing were slightly weakened the moment it was put in the mouth.
1 point: Compared to the control, the vegetable-derived umami and fresh aroma of the dressing weakened the moment it was put in the mouth.
<呈味の広がり>
 5点:コントロールと比べ、ドレッシング本来のうま味が口全体に広がった。
 4点:コントロールと比べ、ドレッシング本来のうま味が口に広がった。
 3点:コントロールと比べ、ドレッシング本来のうま味が同程度であった。
 2点:コントロールと比べ、ドレッシング本来のうま味がやや弱かった。
 1点:コントロールと比べ、ドレッシング本来のうま味が弱かった。
<Expansion of taste>
5 points: The umami inherent in the dressing spread throughout the mouth compared to the control.
4 points: Compared to the control, the umami inherent in the dressing spread in the mouth.
3 points: The original umami taste of the dressing was comparable to that of the control.
2 points: The original umami taste of the dressing was slightly weaker than the control.
1 point: The original umami taste of the dressing was weaker than that of the control.
<雑味>
 5点:コントロールと比べ、雑味が弱まった。
 4点:コントロールと比べ、雑味がやや弱まった。
 3点:コントロールと比べ、雑味が同程度であった。
 2点:コントロールと比べ、雑味がやや強まった。
 1点:コントロールと比べ、雑味が強まった。
<Miscellaneous taste>
5 points: Miscellaneous taste was weakened compared to the control.
4 points: The rough taste was slightly weakened compared to the control.
3 points: Unpleasant taste was comparable to that of the control.
2 points: The rough taste was slightly stronger than the control.
1 point: Miscellaneous taste was stronger than the control.
<総合評価(全体の美味しさ)>
 5点:コントロールと比べ、全体的にドレッシングの風味がかなり向上した。
 4点:コントロールと比べ、全体的にドレッシングの風味が向上した。
 3点:コントロールと比べ、全体的にドレッシングの風味が同程度であった。
 2点:コントロールと比べ、全体的にドレッシングの風味がやや弱かった。
 1点:コントロールと比べ、全体的にドレッシングの風味が弱かった。
<Comprehensive evaluation (overall taste)>
5 points: Compared to the control, the flavor of the dressing was considerably improved as a whole.
4 points: The flavor of the dressing was improved overall compared to the control.
3 points: Overall flavor of the dressing was comparable to that of the control.
2 points: The flavor of the dressing was slightly weak overall compared to the control.
1 point: The flavor of the dressing was weak overall compared to the control.
 各試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)の各含有量(ppm)、(A)/(B)、(A)/(C)、及び官能評価結果を表2に示す。 Each content (ppm) of terpinyl acetate (A), glutamic acid (B), inosinic acid (C), (A) / (B), (A) / (C), and functional Table 2 shows the evaluation results.
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000002
 表2に示されるように、ドレッシングについては、酢酸テルピニルの含有量が0.00000001~1ppmの範囲内で、風味のインパクト、呈味の広がり、全体の美味しさに優れ、野菜由来の雑味も感じられなかった(試験品11~15)。コントロールがコクと、呈味成分が相対的に多く、特にうま味が全体的に強いが、風味にシャープさが増し、フレッシュ感も得られた。酢酸テルピニルの含有量が100ppmの場合(試験品10)、風味のインパクト、呈味の広がり、全体の美味しさがやや低下したが、試験例1のつゆによる評価(試験品1)ほど顕著でなく、許容範囲であった。 As shown in Table 2, when the content of terpinyl acetate is in the range of 0.00000001 to 1 ppm, the dressing is excellent in flavor impact, spread of taste, and overall deliciousness, and contains vegetable-derived miscellaneous taste. Not felt (tests 11-15). The control was full-bodied and contained relatively many taste components, and although umami was particularly strong overall, the flavor was sharper and fresher. When the terpinyl acetate content was 100 ppm (test product 10), the impact of flavor, the spread of taste, and the overall deliciousness were slightly reduced, but not as markedly as the evaluation by the soup in Test Example 1 (test product 1). , was acceptable.
(試験例3)グルタミン酸含有量の影響(つゆによる評価)
(1)試験品の調製
 酢酸テルピニル(井上香料社製、Specification:100%)を1質量%になるよう水に溶解した。この溶液を更に水にて希釈し、1ppm酢酸テルピニル溶液を調製した。
(Test Example 3) Effect of glutamic acid content (evaluation by soup)
(1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
 表3に示す配合量(質量%)に従い、鰹節だし、濃口醤油、砂糖、本みりん、食塩、グルタミン酸ナトリウム、酢酸テルピニル溶液、水を混合し、試験品16~20のつゆを調製した。また、試験品16~20の各つゆに酢酸テルピニル溶液を添加しないつゆをコントロールとした。 Dried bonito stock, dark soy sauce, sugar, mirin, salt, sodium glutamate, terpinyl acetate solution, and water were mixed according to the blending amounts (mass%) shown in Table 3 to prepare test products 16 to 20. In addition, a control was prepared by adding no terpinyl acetate solution to each of the test products 16 to 20.
 調製した各試験品における酢酸テルピニルの含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した各試験品について、官能評価を試験例1と同様の方法にて行った。
(2) Sensory evaluation test Sensory evaluation was performed in the same manner as in Test Example 1 for each test product prepared in (1).
 各試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)の各含有量(ppm)、(A)/(B)、(A)/(C)、及び官能評価結果を表3に示す。 Each content (ppm) of terpinyl acetate (A), glutamic acid (B), inosinic acid (C), (A) / (B), (A) / (C), and functional Table 3 shows the evaluation results.
Figure JPOXMLDOC01-appb-T000003
Figure JPOXMLDOC01-appb-T000003
 表3に示されるように、飲食品中のグルタミン酸の含有量を変えても、グルタミン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/グルタミン酸の含有量(ppm))が本発明の範囲内であると、風味のインパクト、呈味の広がり、全体の美味しさの向上効果がいずれの試験品でも等しく認められた(試験品16~20)。 As shown in Table 3, even if the content of glutamic acid in the food and drink is changed, the ratio of the content of terpinyl acetate to the content of glutamic acid (content of terpinyl acetate (ppm) / content of glutamic acid (ppm) ) is within the scope of the present invention, the impact of flavor, the spread of taste, and the effect of improving overall deliciousness were equally recognized in all test products (test products 16 to 20).
(試験例4)イノシン酸含有量の検討(つゆによる評価)
(1)試験品の調製
 酢酸テルピニル(井上香料社製、Specification:100%)を1質量%になるよう水に溶解した。この溶液を更に水にて希釈し、1ppm酢酸テルピニル溶液を調製した。
(Test Example 4) Examination of inosinic acid content (evaluation by soup)
(1) Preparation of test product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
 表4に示す配合量(質量%)に従い、鰹節だし、濃口醤油、砂糖、本みりん、食塩、イノシン酸ナトリウム、酢酸テルピニル溶液、水を混合し、試験品21~26のつゆを調製した。また、試験品21~26の各つゆに酢酸テルピニル溶液を添加しないつゆをコントロールとした。 Dried bonito stock, dark soy sauce, sugar, mirin, salt, sodium inosinate, terpinyl acetate solution, and water were mixed according to the blending amounts (mass%) shown in Table 4 to prepare test products 21 to 26. In addition, a control was prepared by adding no terpinyl acetate solution to each of the test products 21 to 26.
 調製した各試験品における酢酸テルピニルの含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The terpinyl acetate content, glutamic acid content, and inosinic acid content in each prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した各試験品について、官能評価を試験例1と同様の方法にて行った。
(2) Sensory evaluation test Sensory evaluation was performed in the same manner as in Test Example 1 for each test product prepared in (1).
 各試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)の各含有量(ppm)、(A)/(B)、(A)/(C)、及び官能評価結果を表4に示す。 Each content (ppm) of terpinyl acetate (A), glutamic acid (B), inosinic acid (C), (A) / (B), (A) / (C), and functional Table 4 shows the evaluation results.
Figure JPOXMLDOC01-appb-T000004
Figure JPOXMLDOC01-appb-T000004
 表4に示されるように、飲食品中のイノシン酸の含有量を変えても、イノシン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/イノシン酸の含有量(ppm))が本発明の範囲内であると、風味のインパクト、呈味の広がり、全体の美味しさの向上効果がいずれの試験品でも等しく認められた(試験品21~26)。 As shown in Table 4, even if the content of inosinic acid in the food and drink is changed, the ratio of the content of terpinyl acetate to the content of inosinic acid (content of terpinyl acetate (ppm) / content of inosinic acid (ppm)) is within the range of the present invention, the impact of flavor, the spread of taste, and the effect of improving overall deliciousness were equally recognized in all test products (test products 21 to 26).
(試験例5-1)トマトソースによる評価
(1)試験品の調製
 酢酸テルピニル(井上香料社製、Specification:100%)を1質量%になるよう水に溶解した。この溶液を更に水にて希釈し、1ppmの酢酸テルピニル溶液を調製した。
(Test Example 5-1) Evaluation with Tomato Sauce (1) Preparation of Test Product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
 熱したフライパンに小さじ2のオリーブオイルを入れ、みじん切りにしたにんにく半かけらを炒めた。完熟あらごしトマト(デルモンテ社製)190g、コンソメ顆粒(味の素社製)大さじ1強を加え、3分間煮込んだ。室温で放冷し、トマトソースを得た。このトマトソース99質量%に上記テルピニル溶液を1質量%混合し、試験品27のトマトソースを調製した。また、酢酸テルピニル溶液を添加しないトマトソースをコントロールとした。 Add 2 teaspoons of olive oil to a hot frying pan and stir-fry minced garlic cloves. 190 g of ripe rough tomato (manufactured by Delmonte) and 1 tablespoon of consommé granules (manufactured by Ajinomoto Co.) were added and boiled for 3 minutes. It was allowed to cool at room temperature to obtain a tomato sauce. 1% by mass of the terpinyl solution was mixed with 99% by mass of this tomato sauce to prepare a tomato sauce of test product 27. In addition, a tomato sauce to which no terpinyl acetate solution was added was used as a control.
 調製した試験品における酢酸テルピニルの含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The terpinyl acetate content, glutamic acid content, and inosinic acid content in the prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した試験品について、トマトソースの「風味のインパクト」、「呈味の広がり」、「総合評価(全体の美味しさ)」の各項目の官能評価を、下記の評価基準で行う以外は、試験例1と同様の方法にて行った。
(2) Sensory evaluation test For the test product prepared in (1), sensory evaluation of each item of tomato sauce “flavor impact”, “taste spread”, and “overall evaluation (overall deliciousness)” It was carried out in the same manner as in Test Example 1 except that the following evaluation criteria were used.
<風味のインパクト>
 5点:コントロールと比べ、トマト由来のうま味とフルーティな香りを、口に入れた瞬間に強く感じられた。
 4点:コントロールと比べ、トマト由来のうま味とフルーティな香りを口に入れた瞬間にやや強く感じられた。
 3点:コントロールと比べ、トマト由来のうま味とフルーティな香りを口に入れた瞬間に、同程度に感じられた。
 2点:コントロールと比べ、トマト由来のうま味とフルーティな香りを口に入れた瞬間に、やや弱く感じられた。
 1点:コントロールと比べ、トマト由来のうま味とフルーティな香りを口に入れた瞬間に、弱く感じられた。
<Impact of Flavor>
5 points: Compared to the control, the tomato-derived umami and fruity aroma were strongly felt at the moment of putting in the mouth.
4 points: Compared to the control, the tomato-derived umami and fruity aroma were felt slightly stronger at the moment of putting in the mouth.
3 points: Compared to the control, the umami and fruity aroma derived from tomato were felt at the same level at the moment of putting in the mouth.
2 points: Compared to the control, the tomato-derived umami and fruity aroma were slightly weak at the moment of putting in the mouth.
1 point: Compared to the control, the tomato-derived umami and fruity aroma were felt weaker at the moment of putting in the mouth.
<呈味の広がり>
 5点:コントロールと比べ、トマトソース本来のうま味が口全体に広がった。
 4点:コントロールと比べ、トマトソース本来のうま味が口に広がった。
 3点:コントロールと比べ、トマトソース本来のうま味が同程度であった。
 2点:コントロールと比べトマトソース本来のうま味がやや弱かった。
 1点:コントロールと比べ、トマトソース本来のうま味が弱かった。
<Expansion of taste>
5 points: Compared to the control, the umami inherent in the tomato sauce spread throughout the mouth.
4 points: Compared to the control, the umami inherent in the tomato sauce spread in the mouth.
3 points: The umami inherent in the tomato sauce was comparable to that of the control.
2 points: The umami inherent in the tomato sauce was slightly weaker than the control.
1 point: The umami inherent in the tomato sauce was weak compared to the control.
<総合評価(全体の美味しさ)>
 5点:コントロールと比べ、全体的にトマトソースの風味がかなり向上した。
 4点:コントロールと比べ、全体的にトマトソースの風味が向上した。
 3点:コントロールと比べ、全体的にトマトソースの風味が同程度であった。
 2点:コントロールと比べ、全体的にトマトソースの風味がやや弱かった。
 1点:コントロールと比べ、全体的にトマトソースの風味が弱かった。
<Comprehensive evaluation (overall taste)>
5 points: Compared to the control, the flavor of the tomato sauce was significantly improved as a whole.
4 points: The flavor of the tomato sauce was improved overall compared to the control.
3 points: Overall flavor of tomato sauce was comparable to that of the control.
2 points: The flavor of the tomato sauce was slightly weak overall compared to the control.
1 point: The flavor of the tomato sauce was generally weak compared to the control.
 試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)の各含有量(ppm)、(A)/(B)、(A)/(C)、及び官能評価結果を表5-1に示す。 Terpinyl acetate (A), glutamic acid (B), inosinic acid (C) content (ppm), (A) / (B), (A) / (C), and sensory evaluation of the test product (evaluation sample) The results are shown in Table 5-1.
Figure JPOXMLDOC01-appb-T000005
Figure JPOXMLDOC01-appb-T000005
(試験例5-2)寄せ鍋用つゆによる評価
(1)試験品の調製
 酢酸テルピニル(井上香料社製、Specification:100%)を1質量%になるよう水に溶解した。この溶液を更に水にて希釈し、1ppmの酢酸テルピニル溶液を調製した。
(Test Example 5-2) Evaluation by Soup for Yosenabe (1) Preparation of Test Product Terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare a 1 ppm terpinyl acetate solution.
 魚介類だし(かつお節、昆布、ほたて)4質量%、濃口醤油5質量%、砂糖1質量%、食塩1質量%、酢酸テルピニル溶液1質量%、水88質量%を混合し、試験品28の寄せ鍋用つゆを調製した。また、酢酸テルピニル溶液を添加せず、水89質量%とした寄せ鍋用つゆをコントロールとした。 Mix 4% by mass of seafood stock (bonito flakes, kelp, scallops), 5% by mass of dark soy sauce, 1% by mass of sugar, 1% by mass of salt, 1% by mass of terpinyl acetate solution, and 88% by mass of water, Soup for use was prepared. In addition, a yosenabe dipping sauce containing 89% by mass of water without the addition of the terpinyl acetate solution was used as a control.
 調製した試験品における酢酸テルピニルの含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The terpinyl acetate content, glutamic acid content, and inosinic acid content in the prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した試験品について、寄せ鍋用つゆの「風味のインパクト」、「呈味の広がり」、「総合評価(全体の美味しさ)」の各項目の官能評価を、下記の評価基準で行う
以外は、試験例1と同様の方法にて行った。
(2) Sensory evaluation test For the test product prepared in (1), sensory evaluation of each item of “flavor impact”, “taste spread”, and “overall evaluation (overall deliciousness)” of soup for yosenabe , was carried out in the same manner as in Test Example 1, except that the following evaluation criteria were used.
<風味のインパクト>
 5点:コントロールと比べ、醤油感と醤油の香りを、口に入れた瞬間に強く感じられた。
 4点:コントロールと比べ、醤油感と醤油の香りを口に入れた瞬間にやや強く感じられた。
 3点:コントロールと比べ、醤油感と醤油の香りを口に入れた瞬間に、同程度に感じられた。
 2点:コントロールと比べ、醤油感と醤油の香りを口に入れた瞬間に、やや弱く感じられた。
 1点:コントロールと比べ、醤油感と醤油の香りを口に入れた瞬間に、弱く感じられた。
<Impact of Flavor>
5 points: Compared to the control, the feeling of soy sauce and the aroma of soy sauce were strongly felt at the moment of putting in the mouth.
4 points: Compared to the control, the feeling of soy sauce and the aroma of soy sauce were slightly stronger at the moment of putting in the mouth.
3 points: Compared to the control, the feeling of soy sauce and the aroma of soy sauce were felt at the same level at the moment of putting in the mouth.
2 points: Compared to the control, the feeling of soy sauce and the aroma of soy sauce were felt slightly weaker at the moment of putting in the mouth.
1 point: Compared to the control, the feeling of soy sauce and the aroma of soy sauce were felt weaker at the moment of putting in the mouth.
<呈味の広がり>
 5点:コントロールと比べ、寄せ鍋つゆ本来のうま味が口全体に広がった。
 4点:コントロールと比べ、寄せ鍋つゆ本来のうま味が口に広がった。
 3点:コントロールと比べ、寄せ鍋つゆ本来のうま味が同程度であった。
 2点:コントロールと比べ、寄せ鍋つゆ本来のうま味がやや弱かった。
 1点:コントロールと比べ、寄せ鍋つゆ本来のうま味が弱かった。
<Expansion of taste>
5 points: Compared to the control, the umami inherent in the yosenabe soup spread throughout the mouth.
4 points: Compared to the control, the umami inherent in the yosenabe soup spread in the mouth.
3 points: Compared to the control, the umami inherent in the yosenabe soup was at the same level.
2 points: Compared to the control, the umami inherent in the yosenabe soup was slightly weaker.
1 point: Compared to the control, the umami inherent in the yosenabe soup was weak.
<総合評価(全体の美味しさ)>
 5点:コントロールと比べ、全体的に寄せ鍋つゆの風味がかなり向上した。
 4点:コントロールと比べ、全体的に寄せ鍋つゆの風味が向上した。
 3点:コントロールと比べ、全体的に寄せ鍋つゆの風味が同程度であった。
 2点:コントロールと比べ、全体的に寄せ鍋つゆの風味がやや弱かった。
 1点:コントロールと比べ、全体的に寄せ鍋つゆの風味が弱かった。
<Comprehensive evaluation (overall taste)>
5 points: Compared to the control, the flavor of the yosenabe soup was significantly improved overall.
4 points: Overall, the flavor of the yosenabe soup was improved compared to the control.
3 points: Overall flavor of yosenabe soup was comparable to control.
2 points: Overall flavor of yosenabe soup was slightly weaker than control.
1 point: Overall flavor of yosenabe soup was weak compared to control.
 試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)の各含有量(ppm)、(A)/(B)、(A)/(C)、及び官能評価結果を表5-2に示す。 Terpinyl acetate (A), glutamic acid (B), inosinic acid (C) content (ppm), (A) / (B), (A) / (C), and sensory evaluation of the test product (evaluation sample) The results are shown in Table 5-2.
Figure JPOXMLDOC01-appb-T000006
Figure JPOXMLDOC01-appb-T000006
 表5-1、表5-2に示されるように、飲食品の好ましい態様である液状調味料(トマトソース、寄せ鍋つゆ)において、呈味成分に対して酢酸テルピニルを所定量添加することによって、風味のインパクト、呈味の広がり、全体の美味しさの向上効果が認められた(試験品27、28)。トマトソースは、トマト由来の旨味と香りが高まることで、酸味とのバランスが得られ、まとまりのある風味となった。寄せ鍋用つゆは、醤油風味をシャープに感じることで、フレッシュで雑味が少ない良好な醤油の風味が得られた。 As shown in Tables 5-1 and 5-2, in liquid seasonings (tomato sauce, yosenabe sauce), which are preferred embodiments of foods and drinks, by adding a predetermined amount of terpinyl acetate to the taste component, Flavor impact, spread of taste, and effect of improving overall deliciousness were recognized (test products 27 and 28). In the tomato sauce, the tomato-derived umami and aroma were enhanced, and the balance with the sourness was obtained, resulting in a coherent flavor. The dipping sauce for yosenabe had a sharp soy sauce flavor, and a good soy sauce flavor was obtained that was fresh and had little miscellaneous taste.
(試験例6-1)パスタによる評価
(1)試験品の調製
 試験例5-1で調製したトマトソース(試験品27)、及びそのコントロールを60℃に加温した。乾燥パスタ100重量部を茹でた後、当該パスタに上記試験品27のトマトソースを150重量部かけ混ぜ合わせたトマト風味スパゲティを試験品29とした。また、試験品27に対するコントロールを使用して同様に調製したトマト風味スパゲティをコントロールとした。
(Test Example 6-1) Evaluation with pasta (1) Preparation of test product The tomato sauce (test product 27) prepared in Test Example 5-1 and its control were heated to 60°C. After boiling 100 parts by weight of dry pasta, 150 parts by weight of the tomato sauce of the above test product 27 was mixed with the pasta to prepare tomato-flavored spaghetti as test product 29. Also, tomato-flavored spaghetti prepared in the same manner as the control for test product 27 was used as a control.
(2)官能評価試験
 (1)で調製した各試験品について、官能評価を試験例5-1と同様の方法にて行った。
(2) Sensory Evaluation Test Each test product prepared in (1) was subjected to sensory evaluation in the same manner as in Test Example 5-1.
(試験例6-2)レタスサラダによる評価
(1)試験品の調製
 レタス2枚分を3cm角程度に手でちぎり、ミニトマトを半分にカットし混合後、試験例2で調製したノンオイルドレッシング(試験品12)小さじ1/2重量部をかけたレタスサラダを試験品30とした。また、試験品12に対するコントロールを使用して同様に調製したレタスサラダをコントロールとした。
(Test Example 6-2) Evaluation with lettuce salad (1) Preparation of test product Tear two pieces of lettuce by hand into about 3 cm squares, cut mini tomatoes in half and mix, then prepare the non-oil dressing prepared in Test Example 2 ( Test Item 12) Test Item 30 was lettuce salad with ½ tsp. A lettuce salad prepared in the same manner as the control for Test Product 12 was also used as a control.
(2)官能評価試験
 (1)で調製した各試験品について、官能評価を試験例2と同様の方法にて行った。
(2) Sensory evaluation test Sensory evaluation was performed in the same manner as in Test Example 2 for each test product prepared in (1).
(試験例6-3、6-4)(うどん、そうめんによる評価)
(1)試験品の調製
 試験例1で調製したつゆ(試験品4)、及びコントロールをそれぞれ90℃に加熱した。市販のうどん1玉(180g)を茹でた後、上記試験品4のつゆを50重量部かけて混ぜ合わせたかけうどんを試験品31とした。また、試験品4に対するコントロールを使用して同様に調製したかけうどんをコントロールとした。
(Test Examples 6-3, 6-4) (Evaluation by Udon and Somen)
(1) Preparation of test product The soup (test product 4) prepared in Test Example 1 and the control were each heated to 90°C. Test product 31 was kake udon prepared by boiling one ball (180 g) of commercially available udon noodles and then adding 50 parts by weight of the broth of test product 4 above and mixing them. Kake-udon prepared in the same manner as the control for test product 4 was used as a control.
 また、試験例1で調製したつゆ(試験品4)、及びコントロールをそれぞれ冷蔵庫で10℃に冷却した。市販のそうめんを茹で、冷水にさらした後、上記の冷却した試験品4のつゆを25mLかけた冷やしそうめんを試験品32とした。また、試験品4に対するコントロールを使用して同様に調製したそうめんをコントロールとした。 In addition, the soup (test product 4) prepared in Test Example 1 and the control were each cooled to 10°C in a refrigerator. Test product 32 was chilled somen noodles prepared by boiling commercially available somen noodles, exposing them to cold water, and pouring 25 mL of the cooled soup of test product 4 above. In addition, somen noodles prepared in the same manner as the control for test product 4 were used as a control.
(2)官能評価試験
 (1)で調製した各試験品について、官能評価を試験例1と同様の方法にて行った。
(2) Sensory evaluation test Sensory evaluation was performed in the same manner as in Test Example 1 for each test product prepared in (1).
(試験例6-5)寄せ鍋による評価
(1)試験品の調製
 試験例5-2で調製した寄せ鍋用つゆ(試験品28)、及びコントロールをそれぞれ95℃に加熱した。3cm角にカットした鶏もも肉100g、白菜1/8個、長ネギ1本、しいたけ2つ、水菜2束、豆腐150gを、上記試験品28の寄せ鍋用つゆに加え、蓋をして具材に火が通るまで煮込んだ寄せ鍋を試験品33とした。また、試験品28に対するコントロールを使用して同様に調製した寄せ鍋をコントロールとした。
(Test Example 6-5) Evaluation by mixed pot (1) Preparation of test product The soup for mixed pot (test product 28) prepared in Test Example 5-2 and the control were each heated to 95°C. 100 g of chicken thigh meat cut into 3 cm squares, 1/8 Chinese cabbage, 1 long onion, 2 shiitake mushrooms, 2 bundles of mizuna, and 150 g of tofu are added to the dipping sauce for the yosenabe of test product 28, and the ingredients are heated with a lid. Test product 33 was a hot pot stewed until it was cooked through. In addition, a casserole prepared in the same manner as the control for test product 28 was used as a control.
(2)官能評価試験
 (1)で調製した各試験品について、官能評価を試験例5-2と同様の方法にて行った。
(2) Sensory Evaluation Test Each test product prepared in (1) was subjected to sensory evaluation in the same manner as in Test Example 5-2.
 試験例6-1~6-5の官能評価結果を表6に示す。 Table 6 shows the sensory evaluation results of Test Examples 6-1 to 6-5.
Figure JPOXMLDOC01-appb-T000007
Figure JPOXMLDOC01-appb-T000007
 表6に示されるように、そのまま喫食に供される各種飲食品(パスタ、サラダ、うどん、そうめん、寄せ鍋)においても、呈味成分に対して酢酸テルピニルを所定量添加することによって、風味のインパクト、呈味の広がり、全体の美味しさの向上効果が認められた(試験品29~33)。パスタ、サラダは、料理全体にフレッシュ感が得られた。うどん、そうめん、及び寄せ鍋用つゆは、使用温度に関わらず、醤油の雑味が少なく、フレッシュな醤油風味となった。 As shown in Table 6, even in various foods and drinks (pasta, salad, udon, somen, yose-nabe) that are served as they are, by adding a predetermined amount of terpinyl acetate to the taste components, the impact of the flavor is increased. , broadening of the taste, and the effect of improving the overall deliciousness were observed (test products 29 to 33). Pasta and salad gave a fresh feeling to the whole dish. The soup for udon, somen, and yosenabe had a fresh soy sauce flavor with little soy sauce flavor, regardless of the temperature of use.
(試験例7-1)大豆ミートによる評価
(1)試験品の調製
 乾燥パセリ(エスビー社製)を65℃の熱湯に対し2質量%となるように加え、65℃で15分間静置した後、ろ紙(No.2)にてろ過し、室温まで放置冷却して抽出液を得た。さらに得られた抽出液を20%炭酸ナトリウムでpH10に調整したアルカリ性の抽出液を得た(酢酸テルピニル含有量46ppb)。市販品の大豆ミート33gを沸騰水で5分間茹でたのち、100gまで水気を絞り上げた。
(Test Example 7-1) Evaluation using soybean meat (1) Preparation of test product Dried parsley (manufactured by SB Co., Ltd.) was added to hot water at 65 ° C. so that it would be 2% by mass, and left to stand at 65 ° C. for 15 minutes. and filter paper (No. 2), and left to cool to room temperature to obtain an extract. Further, the obtained extract was adjusted to pH 10 with 20% sodium carbonate to obtain an alkaline extract (terpinyl acetate content: 46 ppb). After boiling 33 g of commercially available soybean meat in boiling water for 5 minutes, the water was squeezed to 100 g.
 市販品の大豆ミート33gを沸騰水で5分間茹でたのち、100gまで水気を絞り上げた。下記表7-1に示す配合量(質量%)に従い、茹でた大豆ミート、醤油、砂糖、酒、酢酸テルピニル溶液又はパセリ抽出液を混合し、試験品34、35、36の味付け大豆ミートを調製した。また、酢酸テルピニル溶液又はパセリ抽出液を添加せず、水1質量%を添加した大豆ミートをコントロールとした。 After boiling 33g of commercially available soybean meat in boiling water for 5 minutes, the water was squeezed out to 100g. Boiled soy meat, soy sauce, sugar, sake, terpinyl acetate solution, or parsley extract are mixed according to the blending amounts (% by mass) shown in Table 7-1 below to prepare seasoned soy meat of test products 34, 35, and 36. bottom. Also, soybean meat to which 1% by mass of water was added without adding terpinyl acetate solution or parsley extract was used as a control.
 調製した各試験品における酢酸テルピニル、アピゲニン、ルテオリンの各含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The contents of terpinyl acetate, apigenin, luteolin, glutamic acid, and inosinic acid in each prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した各試験品について、大豆ミートの「風味のインパクト」、「呈味の広がり」、「総合評価(全体の美味しさ)」の各項目の官能評価を、下記の評価基準で行う以外は、試験例1と同様の方法にて行った。
(2) Sensory evaluation test For each test product prepared in (1), sensory evaluation of each item of “flavor impact”, “taste spread”, and “overall evaluation (overall deliciousness)” of soy meat was performed. , was carried out in the same manner as in Test Example 1, except that the following evaluation criteria were used.
<風味のインパクト>
 5点:コントロールと比べ、大豆ミートの肉っぽさと醤油の香りを口に入れた瞬間に強く感じられた。
 4点:コントロールと比べ、大豆ミートの肉っぽさと醤油の香りを口に入れた瞬間にやや強く感じられた。
 3点:コントロールと比べ、大豆ミートの肉っぽさと醤油の香りを口に入れた瞬間に、同程度に感じられた。
 2点:コントロールと比べ、大豆ミートの肉っぽさと醤油の香りを口に入れた瞬間に、やや弱く感じられた。
 1点:コントロールと比べ、大豆ミートの肉っぽさと醤油の香りを口に入れた瞬間に、弱く感じられた。
<Impact of Flavor>
5 points: Compared to the control, the meatiness of soybean meat and the aroma of soy sauce were strongly felt at the moment of putting in the mouth.
4 points: Compared to the control, the fleshiness of soybean meat and the aroma of soy sauce were felt slightly stronger at the moment of putting in the mouth.
3 points: Compared to the control, the meatiness of soybean meat and the aroma of soy sauce were felt at the same level at the moment of putting in the mouth.
2 points: Compared to the control, the meatiness of soybean meat and the aroma of soy sauce were felt slightly weaker at the moment of putting in the mouth.
1 point: Compared to the control, the meatiness of soybean meat and the aroma of soy sauce were weakly felt at the moment of putting in the mouth.
<呈味の広がり>
 5点:コントロールと比べ、大豆ミートのうま味が口全体に広がった。
 4点:コントロールと比べ、大豆ミートのうま味が口に広がった。
 3点:コントロールと比べ、大豆ミートのうま味が同程度であった。
 2点:コントロールと比べ、大豆ミートのうま味がやや弱かった。
 1点:コントロールと比べ、大豆ミートのうま味が弱かった。
<Expansion of taste>
5 points: The umami of soybean meat spread throughout the mouth compared to the control.
4 points: The umami of soybean meat spread in the mouth compared to the control.
3 points: The umami of soybean meat was comparable to that of the control.
2 points: The umami of soybean meat was slightly weaker than that of the control.
1 point: The umami of soybean meat was weaker than that of the control.
<総合評価(全体の美味しさ)>
 5点:コントロールと比べ、全体的に美味しさがかなり向上した。
 4点:コントロールと比べ、全体的に美味しさがやや向上した。
 3点:コントロールと比べ、全体的に美味しさが同程度であった。
 2点:コントロールと比べ、全体的に美味しさがやや弱かった。
 1点:コントロールと比べ、全体的に美味しさがかなり弱かった。
<Comprehensive evaluation (overall taste)>
5 points: Compared to the control, the overall deliciousness was considerably improved.
4 points: Compared to the control, overall taste is slightly improved.
3 points: Taste was on the same level as the control as a whole.
2 points: Overall taste was slightly weaker than the control.
1 point: Taste was considerably weaker overall than the control.
 各試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)、アピゲニン、ルテオリンの各含有量(ppm)、(A)/(B)、(A)/(C)、及び官能評価結果を表7-1に示す。 Contents (ppm) of terpinyl acetate (A), glutamic acid (B), inosinic acid (C), apigenin, and luteolin in each test product (evaluation sample), (A)/(B), (A)/(C) ), and the sensory evaluation results are shown in Table 7-1.
Figure JPOXMLDOC01-appb-T000008
Figure JPOXMLDOC01-appb-T000008
(試験例7-2)冷凍ねぎとろによる評価
(1)試験品の調製
 パセリ抽出液は試験例7-1と同じものを使用した。下記表7-2に示す配合量(質量%)に従い、解凍した冷凍ねぎとろ、醤油、パセリ抽出液を混合し、試験品37の味付けねぎとろを調整後、空気に触れないようにして一晩置いた。また、パセリ抽出液を添加せず、水を等量添加した味付けねぎとろをコントロールとした。
(Test Example 7-2) Evaluation by Frozen Green Onion Toro (1) Preparation of Test Item The same parsley extract as in Test Example 7-1 was used. According to the blending amount (mass%) shown in Table 7-2 below, mix the thawed frozen Negitoro, soy sauce, and parsley extract, adjust the seasoned Negitoro of Test Product 37, and then overnight without touching the air. placed. Also, a seasoned green onion toro to which an equal amount of water was added without adding the parsley extract was used as a control.
 調製した各試験品における酢酸テルピニル、アピゲニン、ルテオリンの各含有量、グルタミン酸の含有量、及びイノシン酸の含有量を参考例に従って測定した。 The contents of terpinyl acetate, apigenin, luteolin, glutamic acid, and inosinic acid in each prepared test product were measured according to Reference Examples.
(2)官能評価試験
 (1)で調製した各試験品について、味付けねぎとろの「風味のインパクト」、「呈味の広がり」、「総合評価(全体の美味しさ)」の各項目の官能評価を、下記の評価基準で行う以外は、試験例1と同様の方法にて行った。
(2) Sensory evaluation test For each test product prepared in (1), sensory evaluation of each item of “flavor impact”, “taste spread”, and “overall evaluation (overall deliciousness)” of seasoned negitoro was performed in the same manner as in Test Example 1, except that the following evaluation criteria were used.
<風味のインパクト>
 5点:コントロールと比べ、ねぎとろの魚らしいうま味、醤油の香りを口に入れた瞬間に強く感じられた。
 4点:コントロールと比べ、ねぎとろの魚らしいうま味、醤油の香りの香りを口に入れた瞬間にやや強く感じられた。
 3点:コントロールと比べ、ねぎとろの魚らしいうま味、醤油の香りの香りを口に入れた瞬間に、同程度に感じられた。
 2点:コントロールと比べ、ねぎとろの魚らしいうま味、醤油の香りの香りを口に入れた瞬間に、やや弱く感じられた。
 1点:コントロールと比べ、ねぎとろの魚らしいうま味、醤油の香りの香りを口に入れた瞬間に、弱く感じられた。
<Impact of Flavor>
5 points: Compared to the control, the fish-like umami of negi-toro and the scent of soy sauce were strongly felt at the moment of putting in the mouth.
4 points: Compared to the control, the fish-like umami of green onion and tuna and the scent of soy sauce were felt slightly stronger at the moment of putting in the mouth.
3 points: Compared to the control, the fish-like umami of green onion and the fragrance of soy sauce were felt at the same level at the moment of putting in the mouth.
2 points: Compared to the control, the fish-like umami of green onion and the scent of soy sauce were slightly felt at the moment of putting in the mouth.
1 point: Compared to the control, the fish-like umami of negi-toro (roasted onion) and the scent of soy sauce were felt weaker at the moment of putting in the mouth.
<呈味の広がり>
 5点:コントロールと比べ、ねぎとろのうま味が口全体に広がった。
 4点:コントロールと比べ、ねぎとろのうま味が口に広がった。
 3点:コントロールと比べ、ねぎとろのうま味が同程度であった。
 2点:コントロールと比べ、ねぎとろのうま味がやや弱かった。
 1点:コントロールと比べ、ねぎとろのうま味が弱かった。
<Expansion of taste>
5 points: Compared to the control, the umami of green onion paste spread throughout the mouth.
4 points: Compared to the control, the umami of green onion paste spread in the mouth.
3 points: Compared with the control, the savory taste of green onion and toro was at the same level.
2 points: Compared with the control, the savory taste of the green onion toro was slightly weaker.
1 point: The green onion toro had a weak umami taste compared to the control.
<総合評価(全体の美味しさ)>
 5点:コントロールと比べ、全体的に美味しさがかなり向上した。
 4点:コントロールと比べ、全体的に美味しさがやや向上した。
 3点:コントロールと比べ、全体的に美味しさが同程度であった。
 2点:コントロールと比べ、全体的に美味しさがやや弱かった。
 1点:コントロールと比べ、全体的に美味しさがかなり弱かった。
<Comprehensive evaluation (overall taste)>
5 points: Compared to the control, the overall deliciousness was considerably improved.
4 points: Compared to the control, overall taste is slightly improved.
3 points: Taste was on the same level as the control as a whole.
2 points: Overall taste was slightly weaker than the control.
1 point: Taste was considerably weaker overall than the control.
 各試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)、アピゲニン(D)、ルテオリン(E)の各含有量(ppm)、(A)/(B)、(A)/(C)、(D)/(A)、(E)/(A)、(E)/(D)、及び官能評価結果を表7-2に示す。 Each content (ppm) of terpinyl acetate (A), glutamic acid (B), inosinic acid (C), apigenin (D), and luteolin (E) in each test product (evaluation sample), (A)/(B), (A)/(C), (D)/(A), (E)/(A), (E)/(D), and sensory evaluation results are shown in Table 7-2.
Figure JPOXMLDOC01-appb-T000009
Figure JPOXMLDOC01-appb-T000009
(試験例8)酢酸テルピニル、アピゲニン、ルテオリン含有量の検討(つゆによる評価)
(1)試験品の調製
 アピゲニン(Xi’an Natural Field Bio-Technique Co., Ltd、Specification:98%)を1質量%になるよう水に溶解した。この溶液を更に水にて希釈し、表8-1に記載の濃度のアピゲニン溶液を調製した。同様にルテオリン(Xi’an Natural Field Bio-Technique Co., Ltd、Specification:98%)、酢酸テルピニル(井上香料社製、Specification:100%)を1質量%になるよう水に溶解し、更に水にて希釈することで表8-2、表8-3に記載の濃度の各溶液を調製した。
(Test Example 8) Examination of terpinyl acetate, apigenin, and luteolin content (evaluation by soup)
(1) Preparation of test product Apigenin (Xi'an Natural Field Bio-Technique Co., Ltd., Specification: 98%) was dissolved in water to a concentration of 1% by mass. This solution was further diluted with water to prepare an apigenin solution having the concentration shown in Table 8-1. Similarly, luteolin (Xi'an Natural Field Bio-Technique Co., Ltd, Specification: 98%) and terpinyl acetate (manufactured by Inoue Koryo Co., Ltd., Specification: 100%) were dissolved in water to 1% by mass, and further water Each solution with the concentration shown in Table 8-2 and Table 8-3 was prepared by diluting with .
 表8-1~8-3に示す配合量(質量%)に従い、鰹節だし、濃口醤油、砂糖、本みりん、食塩、アピゲニン溶液、ルテオリン溶液、酢酸テルピニル溶液、水を混合し、試験品38~53のつゆを調製した。また、鰹節だし、濃口醤油、砂糖、本みりん、食塩の配合量は同じで、アピゲニン溶液、ルテオリン溶液、酢酸テルピニル溶液を添加せず、水66.5質量%としたつゆをコントロールとした。 According to the blending amounts (mass%) shown in Tables 8-1 to 8-3, dried bonito stock, dark soy sauce, sugar, mirin, salt, apigenin solution, luteolin solution, terpinyl acetate solution, and water were mixed, and test products 38 to 53 soups were prepared. A control soup was prepared with the same amounts of dried bonito stock, dark soy sauce, sugar, mirin, and salt, without the addition of apigenin solution, luteolin solution, and terpinyl acetate solution, and with 66.5% by mass of water.
(2)官能評価試験
 (1)で調製した試験品について、寄せ鍋用つゆの「風味のインパクト」、「呈味の広がり」、「総合評価(全体の美味しさ)」の各項目の官能評価を、試験例5-2と同様の評価基準にて行った。
(2) Sensory evaluation test For the test product prepared in (1), sensory evaluation of each item of “flavor impact”, “taste spread”, and “overall evaluation (overall deliciousness)” of soup for yosenabe , was performed according to the same evaluation criteria as in Test Example 5-2.
 各試験品(評価サンプル)の酢酸テルピニル(A)、グルタミン酸(B)、イノシン酸(C)、アピゲニン(D)、ルテオリン(E)の各含有量(ppm)、(D)/(A)、(E)/(A)、(E)/(D)、及び官能評価結果を表8-1~8-3に示す。 Each content (ppm) of terpinyl acetate (A), glutamic acid (B), inosinic acid (C), apigenin (D), and luteolin (E) in each test product (evaluation sample), (D)/(A), (E)/(A), (E)/(D), and sensory evaluation results are shown in Tables 8-1 to 8-3.
Figure JPOXMLDOC01-appb-T000010
Figure JPOXMLDOC01-appb-T000010
Figure JPOXMLDOC01-appb-T000011
Figure JPOXMLDOC01-appb-T000011
Figure JPOXMLDOC01-appb-T000012
Figure JPOXMLDOC01-appb-T000012
 本発明は、調味料などの飲食品の製造分野において利用できる。
 本明細書で引用した全ての刊行物、特許及び特許出願をそのまま参考として本明細書に組み入れるものとする。
INDUSTRIAL APPLICABILITY The present invention can be used in the field of manufacturing foods and drinks such as seasonings.
All publications, patents and patent applications cited herein are hereby incorporated by reference in their entirety.

Claims (17)

  1.  酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上と呈味成分を含有する、呈味と香りが向上した飲食品。 Food and drink with improved taste and aroma, containing one or more selected from terpinyl acetate, apigenin, and luteolin and a taste component.
  2.  酢酸テルピニルの含有量が0.00000001ppm以上10ppm以下である、請求項1に記載の飲食品。 The food or drink according to claim 1, wherein the content of terpinyl acetate is 0.00000001 ppm or more and 10 ppm or less.
  3.  アピゲニンの含有量が0.00001ppm以上10ppm以下である、請求項1に記載の飲食品。 The food or drink according to claim 1, wherein the content of apigenin is 0.00001 ppm or more and 10 ppm or less.
  4.  ルテオリンの含有量が0.00001ppm以上100ppm以下である、請求項1に記載の飲食品。 The food or drink according to claim 1, wherein the content of luteolin is 0.00001 ppm or more and 100 ppm or less.
  5.  前記呈味成分が、うま味成分、塩味成分、甘味成分、及び酸味成分から選ばれる少なくとも1種である、請求項1~4のいずれか1項に記載の飲食品。 The food or drink according to any one of claims 1 to 4, wherein the taste component is at least one selected from an umami component, a salty component, a sweet component, and a sour component.
  6.  前記呈味成分が、うま味成分であって、該うま味成分が、グルタミン酸、イノシン酸、及びコハク酸から選ばれる少なくとも1種である、請求項1~5のいずれか1項に記載の飲食品。 The food or drink according to any one of claims 1 to 5, wherein the taste component is an umami component, and the umami component is at least one selected from glutamic acid, inosinic acid, and succinic acid.
  7.  前記呈味成分が、グルタミン酸又はイノシン酸のいずれか1種又は2種のうま味成分であって、以下の(a1)及び(a2)を満たす、請求項1~6のいずれか1項に記載の飲食品。
     (a1) 飲食品中のグルタミン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/グルタミン酸の含有量(ppm))が、5.0×10-12~1×10-2
     (a2) 飲食品中のイノシン酸の含有量に対する酢酸テルピニルの含有量の比率(酢酸テルピニルの含有量(ppm)/イノシン酸の含有量(ppm))が6.0×10-11~0.2
    7. The taste component according to any one of claims 1 to 6, wherein the umami component is either one or two of glutamic acid and inosinic acid, and satisfies the following (a1) and (a2): food and drink.
    (a1) The ratio of the content of terpinyl acetate to the content of glutamic acid in the food or drink (content of terpinyl acetate (ppm)/content of glutamic acid (ppm)) is 5.0×10 −12 to 1×10 -2
    (a2) The ratio of the content of terpinyl acetate to the content of inosinic acid in the food or drink (content of terpinyl acetate (ppm)/content of inosinic acid (ppm)) is 6.0×10 −11 to 0.0. 2
  8.  前記呈味成分が、グルタミン酸又はイノシン酸のいずれか1種又は2種のうま味成分であって、以下の(b1)及び(b2)を満たす、請求項1~6のいずれか1項に記載の飲食品。
     (b1) 飲食品中のグルタミン酸の含有量に対するアピゲニンの含有量の比率(アピゲニンの含有量(ppm)/グルタミン酸の含有量(ppm))が、5.0×10-9~1×10-2
     (b2) 飲食品中のイノシン酸の含有量に対するアピゲニンの含有量の比率(アピゲニンの含有量(ppm)/イノシン酸の含有量(ppm))が5.0×10-10~1.0×10-3
    7. The taste component according to any one of claims 1 to 6, wherein the umami component is either one or two of glutamic acid and inosinic acid, and satisfies the following (b1) and (b2): food and drink.
    (b1) The ratio of the apigenin content to the glutamic acid content in the food or drink (apigenin content (ppm)/glutamic acid content (ppm)) is 5.0×10 −9 to 1×10 −2
    (b2) The ratio of the content of apigenin to the content of inosinic acid in the food or drink (content of apigenin (ppm)/content of inosinic acid (ppm)) is 5.0×10 −10 to 1.0× 10-3
  9.  前記呈味成分が、グルタミン酸又はイノシン酸のいずれか1種又は2種のうま味成分であって、以下の(c1)及び(c2)を満たす、請求項1~6のいずれか1項に記載の飲食品。
     (c1) 飲食品中のグルタミン酸の含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/グルタミン酸の含有量(ppm))が、5.0×10-9~1.0×10-1
     (c2) 飲食品中のイノシン酸の含有量に対するルテオリンの含有量の比率(ルテオリンの含有量(ppm)/イノシン酸の含有量(ppm))が5.0×10-10~1.0×10-2
    7. The taste component according to any one of claims 1 to 6, wherein the umami component is either one or two of glutamic acid and inosinic acid, and satisfies the following (c1) and (c2): food and drink.
    (c1) The ratio of the content of luteolin to the content of glutamic acid in the food or drink (luteolin content (ppm) / glutamic acid content (ppm)) is 5.0 × 10 -9 to 1.0 × 10 -1
    (c2) The ratio of the content of luteolin to the content of inosinic acid in the food or drink (luteolin content (ppm)/inosinic acid content (ppm)) is 5.0×10 −10 to 1.0× 10-2
  10.  前記飲食品が、調味料である、請求項1~9のいずれか1項に記載の飲食品。 The food and drink according to any one of claims 1 to 9, wherein the food and drink is a seasoning.
  11.  前記調味料が、液状調味料である、請求項10に記載の飲食品。 The food or drink according to claim 10, wherein the seasoning is a liquid seasoning.
  12.  前記調味料が、つゆ類、たれ類、だし類、エキス類、ソース類、又はドレッシング類である、請求項10又は11に記載の飲食品。 The food or drink according to claim 10 or 11, wherein the seasoning is soups, sauces, soup stocks, extracts, sauces, or dressings.
  13.  前記飲食品が、植物性蛋白質含有食品である、請求項1~9のいずれか1項に記載の飲食品。 The food or drink according to any one of claims 1 to 9, wherein the food or drink is a vegetable protein-containing food.
  14.  酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を、呈味成分を含む飲食品又はその原料に添加することを含む、飲食品の呈味と香りを向上させる方法。 A method for improving the taste and aroma of food and drink, comprising adding one or more selected from terpinyl acetate, apigenin, and luteolin to food and drink containing flavoring components or raw materials thereof.
  15.  下記の段階(i)~(iii)を含む方法で植物素材抽出物を調製することを含む、請求項1~13のいずれか1項に記載の呈味と香りが向上した飲食品の製造方法。
     (i) 酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材を抽出溶媒に添加する段階
     (ii) 植物素材から抽出溶媒にて成分抽出し、酢酸テルピニル、アピゲニン、及びルテオリンから選ばれる1種又は2種以上を含有する植物素材抽出物を得る工程
     (iii) (ii)で得られた植物素材抽出物のpHを相対的に酸性側に調整する段階
    A method for producing a food or drink with improved taste and aroma according to any one of claims 1 to 13, which comprises preparing a plant material extract by a method including the following steps (i) to (iii). .
    (i) A step of adding a plant material containing one or more selected from terpinyl acetate, apigenin, and luteolin to an extraction solvent (ii) Extracting components from the plant material with an extraction solvent, and a step of obtaining a plant material extract containing one or more selected from luteolin (iii) a step of adjusting the pH of the plant material extract obtained in (ii) to be relatively acidic.
  16.  前記段階(ii)の後、植物素材抽出物のpHを6.5以上12.0以下に調整する段階をさらに含む、請求項15に記載の製造方法。 The production method according to claim 15, further comprising the step of adjusting the pH of the plant material extract to 6.5 or more and 12.0 or less after the step (ii).
  17.  調製した植物素材抽出物のpHが1.5以上7.0未満である、請求項15又は16に記載の製造方法。 The production method according to claim 15 or 16, wherein the pH of the prepared plant material extract is 1.5 or more and less than 7.0.
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