WO2023005106A1 - 一种挑针自动复位机构及自动菌落挑选仪 - Google Patents

一种挑针自动复位机构及自动菌落挑选仪 Download PDF

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Publication number
WO2023005106A1
WO2023005106A1 PCT/CN2021/137769 CN2021137769W WO2023005106A1 WO 2023005106 A1 WO2023005106 A1 WO 2023005106A1 CN 2021137769 W CN2021137769 W CN 2021137769W WO 2023005106 A1 WO2023005106 A1 WO 2023005106A1
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Prior art keywords
needle
picking
pick
automatic
fixed
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Ceased
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PCT/CN2021/137769
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English (en)
French (fr)
Inventor
何凯
朱伟
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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Publication of WO2023005106A1 publication Critical patent/WO2023005106A1/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B1/00—Cleaning by methods involving the use of tools
    • B08B1/10—Cleaning by methods involving the use of tools characterised by the type of cleaning tool
    • B08B1/12—Brushes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B1/00—Cleaning by methods involving the use of tools
    • B08B1/20—Cleaning of moving articles, e.g. of moving webs or of objects on a conveyor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/02—Cleaning by the force of jets or sprays
    • B08B3/022—Cleaning travelling work
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/52—Mobile; Means for transporting the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

Definitions

  • the invention relates to the field of colony selection equipment, in particular to an automatic reset mechanism for picking needles and an automatic colony selection instrument.
  • the automatic colony sorting instrument includes a number of picking needles that are evenly spaced in a closed loop, and several picking needles are connected to the same transmission mechanism for driving it to rotate around the closed loop.
  • the driving part that picks and inoculates the target colony is moved downward, the transmission mechanism drives the pick needle to rotate, and the drive part drives the pick needle to move downward to pick or inoculate the colony.
  • the automatic colony picking instrument also includes an automatic reset mechanism for vertically upwardly resetting the picking needle.
  • the automatic reset mechanism includes a connecting plate for fixing with the transmission mechanism, the connecting plate slides along the vertical direction and is connected with a fixing plate for fixing the needle, the connecting plate and the fixing plate both extend along the circumferential direction of the transmission mechanism, the connecting plate and the A spring is arranged between the fixing plates to make the fixing plates drive the picking needles to return vertically upwards.
  • Both the connecting plate and the fixing plate in the related art extend along the circumferential direction of the transmission mechanism.
  • Such an arrangement makes the volume of the automatic reset mechanism relatively large relative to the circumferential direction of the transmission mechanism, which limits the maximum set of automatic reset mechanisms in the same space. The number, which in turn limits the working efficiency of the automatic colony picker, so there is room for improvement.
  • the present application provides an automatic reset mechanism for picking needles and an automatic colony picking instrument.
  • An automatic reset mechanism for picking needles including a connecting piece for fixing with a transmission mechanism, a lifting needle fixing piece is vertically slidably connected to the connecting piece, and the picking needle fixing piece is used for fixing the picking needles, and the picking needles are fixed.
  • the needle automatic reset mechanism also includes an elastic reset member, the two ends of the elastic reset member are respectively connected to the connecting member and the needle-picking fixture, and the needle-picking fixture is provided with a force-receiving part for the power source to abut against the applied force, The elastic restoring part is used to make the needle fixing part drive the needle to reset vertically upward.
  • the power source exerts force on the force-bearing part on the needle fixing part, so that the needle fixing part drives the needle to move vertically downward, picks and inoculates the target colony, and completes the picking of the target colony
  • the pick needle fixing part is reset vertically upward under the action of the elastic reset member, on the basis of realizing the automatic reset of the pick needle, the volume of the pick needle automatic reset mechanism is reduced, and the structure of the pick needle automatic reset mechanism is simplified, The volume ratio of the automatic needle-picking reset mechanism relative to the circumference of the transmission mechanism is reduced, and the maximum number of groups of the needle-picking automatic reset mechanism that can be set along the circumference of the transmission mechanism is increased, thereby improving the working efficiency of the automatic colony picker.
  • the needle automatic reset mechanism further includes a limit portion, the limit portion is located on the moving path of the needle fixing member, and is used to limit the upward movement range of the needle fixing member, and the needle fixing member An abutting piece for abutting against the limiting portion is fixed on the piece, and when the abutting piece abuts against the limiting portion, the elastic reset member is in a stressed state.
  • the needle fixing part drives the needle to move vertically upwards
  • the needle fixing part moves to abut against the limit part, restricting the needle fixing part and the needle from continuing to move upward, and determining the position of the needle
  • the reset position ensures the reset accuracy of the picking needle.
  • the connecting piece is a set that is passed through and fixed to the transmission mechanism in the vertical direction
  • the needle fixing piece includes a guide column coaxially arranged with the connecting piece, and the guiding column slides through the connecting piece
  • the picking pin is fixed at the bottom of the guide column
  • the elastic reset member is set as a compression spring coaxially sleeved on the outside of the guide column
  • an upper stop ring is fixed at the top of the guide column
  • the top of the compression spring rests on the bottom of the guide column.
  • the bottom end of the pressure spring abuts against the connecting piece
  • the abutment piece is the lower stop ring fixed at the bottom end of the guide column.
  • the power source exerts a force on the top end surface of the guide column, so that the guide column drives the picking needle to move vertically downward to realize the picking or inoculation of bacterial colonies.
  • the column resets vertically upward under the action of the compression spring, and when the lower stop ring moves to abut against the bottom end of the connecting piece, the guide column and the pick needle stop moving, limiting the movement stroke of the pick needle, and determining the reset position of the pick needle, ensuring The reset accuracy of the needle; at the same time, through the cooperation of the guide column and the connecting piece, the verticality during the movement of the guide column is guaranteed, thereby ensuring the verticality and repeat positioning accuracy during the movement of the needle.
  • the connecting piece is configured as a linear bearing, and the guide column cooperates with the linear bearing.
  • the bottom end of the guide column is provided with a connection hole coaxial therewith, the connection hole is adapted to the pick pin, and the top end of the pick pin is inserted and fixed in the connection hole.
  • the connecting piece includes a connecting plate for fixing to the transmission mechanism and a fixing plate fixed to the connecting plate, the connecting plate is arranged along a tangential direction perpendicular to the movement track direction of the transmission mechanism, and the needle fixing member It includes a vertically arranged guide rail, the guide rail is slidably connected to the connector, the guide rail is fixed with a connecting strip for fixing with the pick needle, the elastic reset part is set as a tension spring, and the top end of the tension spring is connected to the The connecting part, the bottom end of the tension spring is connected to the connecting bar, the limiting part includes a stop bar fixed on the connecting part, the abutting part is a pulling rod fixed on the connecting bar, and the pulling rod is located at Below the stop bar.
  • the power source exerts a force on the top end surface of the guide rail, so that the guide rail and the connecting bar drive the picking needle to move vertically downward to realize the picking or inoculation of bacterial colonies; after the picking needle completes the picking or inoculation of bacterial colonies,
  • the guide rail and the connecting bar are reset vertically upward under the action of the tension spring.
  • the connecting piece is fixed with a fixed rod corresponding to the position of the top end of the extension spring, the top end of the extension spring is connected to the fixed rod, and the bottom end of the extension spring is connected to the pull rod.
  • tension springs there are two tension springs arranged symmetrically.
  • the stability of the force exerted on the connecting bar and the guide rail is improved, the deviation of the guide rail is reduced, and the verticality during the movement of the guide rail is improved, thereby ensuring the verticality and repetition during the needle picking movement. positioning accuracy.
  • An automatic colony sorting instrument comprising a transmission mechanism, the transmission mechanism including a transmission part arranged in a closed loop and a power part for providing power to the transmission part, and the automatic colony selection instrument also includes a number of picking needle automatic reset mechanisms, Several automatic reset mechanisms for picking needles are evenly distributed along the circumferential direction of the transmission part, the connecting part is fixed on the transmission part, and the bottom end of the lifting needle fixing part is fixed with a picking needle, and the picking needles are vertically arranged;
  • the automatic colony sorting instrument also includes picking stations, inoculation stations and cleaning stations arranged in sequence along the direction of motion track of the transmission member, and the automatic colony picking instrument also includes corresponding to the inoculation station and the picking station.
  • the picking station driving member and the inoculation station driving member are used to provide a power source for vertically moving downwards of the picking needle.
  • Fig. 1 is a schematic diagram showing the overall structure of the automatic colony picking apparatus in Example 1.
  • Fig. 2 is a partial structural schematic diagram showing the structure of the automatic reset mechanism for picking needles in the first embodiment.
  • Fig. 3 is a partial structural schematic diagram showing the structure of the automatic reset mechanism for picking needles in the first embodiment.
  • Fig. 4 is a partial structural schematic diagram showing the cleaning mechanism in the first embodiment.
  • Fig. 5 is a schematic diagram showing the overall structure of the automatic colony picking instrument in Example 2.
  • Fig. 6 is a partial structural schematic diagram showing the structure of the automatic reset mechanism for picking needles in the second embodiment.
  • Fig. 7 is a schematic diagram showing the local structure of the cleaning station in the third embodiment.
  • Embodiment 1 of the present application discloses an automatic reset mechanism for picking needles and an automatic colony selection instrument.
  • the transmission part is a rotating disc 11 arranged in a disc shape
  • the power part is a motor fixed on the worktable 1 and coaxially arranged with the rotating disc 11, and the rotating disc 11 is fixed at the output end of the motor.
  • the transmission part can also be other closed-loop components, and the power part can also be selected according to the setting of the transmission part.
  • the power part can be set It is a synchronous pulley arranged on the workbench 1 at intervals along the movement track direction of the synchronous belt and a motor for driving the synchronous belt to rotate.
  • the outer edge of the rotating disk 11 is evenly distributed with several groups of needle automatic reset mechanisms at intervals along its circumference, and the bottom of any group of needle automatic reset mechanisms is fixed with needles 2 for picking and inoculating bacterial colonies.
  • Pin 2 is set vertically.
  • Workbench 1 is provided with picking station 3, inoculation station 4 and cleaning station 5 successively along the motion track direction of rotating disc 11, and picking station 3, inoculation station 4 and cleaning station 5 are respectively used for feeding
  • the pick 2 picks and inoculates the colony and cleans the pick 2.
  • a support frame 12 is fixed on the workbench 1, and a pick station driver 61 corresponding to the pick station 3 and an inoculation station driver 62 corresponding to the inoculation station 4 are fixed on the support frame 12.
  • the station driver 61 and the inoculation station driver 62 are respectively used to drive the pick needles 2 corresponding to the picking station 3 and the inoculation station 4 to move vertically downwards, so as to realize the picking and inoculation of bacterial colonies.
  • the needle picking automatic reset mechanism includes a connecting piece used to connect with the rotating disk 11, a lifting needle fixing piece that is slidably connected to the connecting piece in the vertical direction, and is used to make the picking needle fixing piece vertically upward.
  • the reset elastic reset member and the stopper for limiting the range of the pick needle 2 moving upward.
  • the connecting piece is a vertical linear bearing 71 , and in this embodiment, the linear bearing 71 is an H-shaped flange linear bearing 71 .
  • the rotating disc 11 is provided with a receiving hole compatible with the linear bearing 71, the bottom end of the linear bearing 71 passes through the receiving hole, the top end of the linear bearing 71 is fixed on the upper surface of the rotating disc 11, and the bottom end of the linear bearing 71 is the The limit part in the automatic reset mechanism of the middle picking needle.
  • the needle pick fixture includes a vertically arranged guide column 72 , the cross section of which is circular, and the guide column 72 is slidably matched with the linear bearing 71 in the vertical direction.
  • An upper stop ring 721 is coaxially fixed on the top of the guide post 72 , and the upper surface of the upper stop ring 721 is flush with the top end surface of the guide post 72 .
  • the elastic reset member is a compression spring 73 coaxially sleeved on the outside of the guide post 72 .
  • a lower stop ring 722 is coaxially fixed on the outer peripheral surface of the bottom end of the guide post 72. There is a distance between the lower surface of the lower stop ring 722 and the bottom end surface of the guide post 72.
  • the lower stop ring 722 is the The abutment in the needle automatic reset mechanism.
  • the guide column 72 drives the pick needle 2 to reset vertically upward under the action of the compression spring 73, and when the lower stop ring 722 moves to abut against the bottom end of the linear bearing 71, the compression spring 73 is in a compressed state, and the pick needle 2 is at this moment.
  • Fully reset state determine the reset position of the needle 2, and ensure the reset accuracy of the needle 2; on the basis of realizing the automatic reset of the needle 2, the overall structure of the automatic reset mechanism of the needle is miniaturized, thereby reducing the automatic The volume ratio of the reset mechanism on the device; at the same time, through the cooperation of the guide column 72 and the linear bearing 71, the verticality during the movement of the guide column 72 is guaranteed, thereby ensuring the verticality and repeated positioning of the pick needle 2 during movement precision.
  • the bottom end of the guide post 72 is provided with a connecting hole 723 coaxially therewith, and the top end of the pick 2 is inserted into and fixed in the connecting hole 723 .
  • the picking station 3 is used for two-dimensional control of the petri dish in the horizontal plane, and the picking station 3 includes a two-dimensional driving mechanism 31 for the petri dish and a petri dish supporting plate 32 for supporting the petri dish.
  • the two-dimensional driving mechanism 31 of the petri dish comprises a lower linear module 311 and an upper linear module 312 connected to the output end of the lower linear module 311, the petri dish supporting plate 32 is fixedly connected to the output end of the upper linear module 312, and the upper linear module
  • the conveying direction of the group 312 and the conveying direction of the lower linear module 311 are perpendicular to each other, the upper linear module 312 and the lower linear module 311 are electrically connected to the controller, and the controller controls the upper linear module 312 and the lower linear module 311
  • the output end moves to realize the adjustment of the position of the petri dish.
  • the support frame 12 is also provided with a camera 13, the camera 13 is electrically connected to the controller, the camera 13 is used to measure the range of the petri dish and take pictures, and then the pictures are sent to the image processing software, and the images with colonies are extracted after software processing.
  • the picking station driver 61 is an electric cylinder arranged vertically, and the electric cylinder is electrically connected to the controller.
  • the column 72 exerts an active force, so that the guide column 72 drives the pick needle 2 to move vertically downwards, thereby realizing the driving of the pick needle 2 .
  • the picking station driving member 61 can also be configured as other actuators capable of dynamically adjusting the stroke.
  • the experimenter In the process of colony picking, in order to obtain the best experimental results, the experimenter needs to pick only the surface of the colony, but because the thickness of the medium in the culture dish is different each time, the distance between the surface of the colony and the pick needle is 2
  • the height of the colony is variable, and the dynamic height of the colony identified by the camera 13 is converted into a dynamic stroke through the electric cylinder, so as to realize the dynamic adjustment of each picking stroke of the picking needle 2, and then realize the control of the height of the colony picking, and further The scientificity and reliability of the experimental results are improved.
  • the inoculation station 4 is used for two-dimensional control of the microplate in the horizontal plane.
  • the inoculation station 4 includes a microplate two-dimensional drive mechanism 41 and a microplate supporting plate 42 for supporting the microplate.
  • the supporting plate 42 is connected to the output end of the microplate two-dimensional drive mechanism 41, and the microplate two-dimensional drive mechanism 41 is electrically connected to the controller.
  • the microplate two-dimensional driving mechanism 41 in the inoculation station 4 has the same structure as the petri dish two-dimensional driving mechanism 31 in the picking station 3, and will not be repeated here.
  • the driving member 62 of the inoculation station is a vertical cylinder
  • the cylinder is electrically connected to the controller
  • the rod end of the cylinder moves down to abut against the top end surface of the guide post 72 to act on the guide post 72.
  • the force makes the guide post 72 drive the pick needle 2 to move vertically downwards, so as to realize the driving of the pick needle 2.
  • the driving member 62 of the inoculation station can also be configured as an electric cylinder or other actuators capable of linear reciprocating motion.
  • the cleaning station 5 includes a cleaning mechanism, a heating mechanism and a cooling mechanism, and the cleaning mechanism, the heating mechanism and the cooling mechanism are arranged in sequence along the movement track direction of the rotating disk 11 .
  • the cleaning mechanism includes a bracket 511 fixed on the workbench 1.
  • a baffle 512 is arranged on the top of the bracket 511.
  • Two baffles 512 are arranged at intervals along the radial direction of the rotating disk 11.
  • Two baffles 512 are formed between opposite side walls. Cleaning channel for pick 2.
  • the baffle 512 is slidingly connected to the bracket 511 along the radial direction of the rotating disk 11.
  • the bracket 511 is provided with an oblong hole, and a locking bolt is threaded through the oblong hole from bottom to top, and the locking bolt is screwed to the baffle 512. At the bottom, locking bolts are used to lock the baffle 512 in a fixed position.
  • First brushes 513 are respectively fixed on the two side walls of the cleaning channel, the bristles of the first brushes 513 are arranged horizontally, and two groups of first brushes 513 are arranged at intervals on the same side of the cleaning channel.
  • a first alcohol nozzle 514 is installed and fixed on the outer baffle 512 , the first alcohol nozzle 514 is located between the two groups of first brushes 513 , and the first alcohol nozzle 514 is arranged along the radial direction of the rotating disk 11 .
  • the top end surface of the bracket 511 is sunken downwards in the middle of the two groups of first brushes 513 to form an inner groove 5111 arranged in a bucket shape.
  • the bottom end of the bracket 511 is equipped with an alcohol guide tube 515, and the top of the alcohol guide tube 515 is connected to the inner groove.
  • the cavity of the groove 5111 is connected.
  • the pick needle 2 When the pick needle 2 rotates with the rotating disk 11, the pick needle 2 enters the disinfection channel, the first brush 513 brushes off the large residue on the pick needle 2, and at the same time, the first alcohol nozzle 514 sprays alcohol outward to the pick needle.
  • the needle 2 is disinfected, and the first hair brush 513 also plays the role of reducing alcohol splashing.
  • the alcohol is discharged through the inner groove 5111 and the alcohol guide tube 515 .
  • the heating mechanism is set as a heat gun 521, and the shower head of the heat gun 521 is arranged vertically upwards, and the heat gun 521 is used to spray hot air to the pick needle 2 passing through its top to dry the pick needle 2.
  • the cooling mechanism is configured as a cold air gun 531, and the nozzle of the cold air gun 531 is arranged vertically upwards, and is used to spray cold air through the pick pin 2 above it to cool the pick pin 2.
  • the rotating disk 11 rotates under the action of the motor, driving the needle automatic reset mechanism and the needle 2 to rotate.
  • the needle 2 rotates to the cleaning mechanism, the heating mechanism and the cooling mechanism to complete Cleaning and disinfection, heating and cooling work to realize the cleaning of pick needle 2;
  • the different picking needles 2 are rotated to directly below the picking station driving member 61 and the inoculation station driving member 62, and the controller controls the rotating disk 11 to stop, and the two-dimensional driving mechanism 31 and The microplate two-dimensional driving mechanism 41 moves the culture dish and the microplate to the directly below the picking needle 2 respectively, and the picking station driving part 61 and the inoculation station driving part 62 apply pressure to the top end faces of the corresponding guide posts 72 respectively. Active force makes the corresponding pick needle 2 move vertically downward to realize the picking and inoculation of bacterial colonies;
  • the guide post 72 After the picking needle 2 completes the picking or inoculation of bacterial colonies, the guide post 72 resets vertically upward under the action of the clip spring 73, and when the lower stop ring 722 moves to abut against the bottom end face of the linear bearing 71, the guide post 72 and Picking needle 2 stops moving, and the reset position of picking needle 2 is determined;
  • an automatic reset mechanism for picking needles and an automatic colony picking instrument differ from Embodiment 1 in that the connector includes a connecting plate 81 arranged in an L shape, and one end of the connecting plate 81 arranged horizontally rotates along the The radial extension of the disk 11 is fixed on the upper surface of the rotating disk 11 by using bolts, and the side wall where one end of the connecting plate 81 is vertically arranged away from the axis of the rotating disk 11 is connected with a fixed plate 82 by bolts. 82 is located at the outer side of the rotating disk 11.
  • the needle fixing member includes a guide rail 83 slidably connected to the side wall of the fixing plate 82 away from the connecting plate 81 , and the guide rail 83 is arranged vertically. The side of the guide rail 83 far away from the fixing plate 82 is connected with a vertically arranged connecting bar 84 by bolts, and the picking needle 2 is fixed on the bottom end of the connecting bar 84 .
  • the rods of the two upper bolts are set as fixed rods 85
  • the rods of the two lower bolts are configured as stop rods 86
  • the two fixed rods 85 are at the same height
  • the two stop rods 86 are at the same height.
  • the elastic reset member is configured as a tension spring 88 , one end of the two tension springs 88 is sleeved on the pull rod 87 , and the other ends of the two tension springs 88 are respectively sleeved on the outside of the two fixing rods 85 .
  • the two stop bars 86 are jointly connected with the same stop bar 89, the stop bar 89 is arranged horizontally and perpendicular to the two stop bars 86, and the two ends of the stop bar 89 are sleeved and fixed on the two stop bars 86 respectively. , the stop bar 89 and the two stop rods 86 are the limiting part in this embodiment.
  • the picking station driver 61 or the inoculation station driver 62 exerts force on the top end surface of the corresponding guide rail 83 respectively, so that the corresponding guide rail 83 and the connecting bar 84 respectively drive the corresponding picking needle 2 to move vertically downward, realizing Picking or inoculation of bacterial colonies; after the picking needle 2 completes the picking or inoculation of bacterial colonies, the guide rail 83 and the connecting bar 84 reset vertically upwards under the action of the tension spring 88, and when the stop rod 86 moves to the stop bar 89
  • the pick needle reset mechanism The overall structure is miniaturized, thereby reducing the volume ratio of the needle automatic reset mechanism on the device.
  • a needle picking automatic reset mechanism and an automatic colony picking instrument are different from Embodiment 1 in that the cleaning mechanism includes a cleaning table 541, and the cleaning table 541 includes two radial intervals along the rotating disk 11. Arranged arc-shaped plates, the arc-shaped plates are coaxially arranged with the rotating disk 11, and a cleaning channel for the pick needle 2 to pass is formed between the opposite side walls of the two arc-shaped plates.
  • the two side walls of the cleaning channel are respectively fixed with second hair brushes 542, the bristles of the second hair brushes 542 are arranged horizontally, and two groups of second hair brushes 542 are arranged at intervals on the same side of the cleaning channel, and the two groups of second hair brushes 542
  • a second alcohol nozzle 543 is installed and fixed in the middle.
  • the heating mechanism includes a heating platform 551 fixed on the workbench 1 , the structure of the heating platform 551 is the same as that of the cleaning platform 541 , and a heating channel 552 is arranged in the heating platform 551 .
  • Heating chips 553 are respectively fixed on the two side walls of the heating channel 552 , and the heating chips 553 extend along the extending direction of the heating channel 552 .
  • the heating chips 553 are set as ceramic heating chips 553 .
  • the cooling mechanism includes a cooling platform 561 fixed on the workbench 1 , the structure of the cooling platform 561 is the same as that of the cleaning platform 541 , and a cooling channel 562 is arranged in the cooling platform 561 .
  • Cooler nozzles 563 are respectively fixed on the two side walls of the cooling channel 562 .
  • the cooler nozzles 563 are arranged along the radial direction of the rotating disk 11 .
  • the pick needle 2 When the pick needle 2 rotates with the rotating disk 11, the pick needle 2 enters the disinfection channel, the second brush 542 brushes off the large residue on the pick needle 2, and at the same time, the second alcohol nozzle 543 sprays alcohol outward to the pick.
  • the needle 2 is cleaned and disinfected.
  • the second brush 542 also plays a role in reducing alcohol splashing.
  • the needle 2 continues to move to the heating channel 552.
  • the heating plate 553 releases heat to dry the needle 2, and the needle 2 Continue to move to the cooling channel 562, the cooler nozzle 563 cools the pick needle 2, and realizes the cleaning, disinfection, heating and cooling of the pick needle 2 on the premise that the pick needle 2 does not need to move up and down, prolonging the life of the pick needle 2
  • the cleaning time reduces the factors of incomplete cleaning and improves the reliability and scientificity of the experimental results.

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Abstract

一种挑针自动复位机构及自动菌落挑选仪，其包括用于与传动机构固定的连接件，连接件上竖向滑移连接有挑针固定件，挑针固定件用于固定挑针，挑针自动复位机构还包括弹性复位件，弹性复位件的两端分别连接于连接件和挑针固定件，挑针固定件上设置有用于动力源抵接施力的受力部，弹性复位件用于使挑针固定件带动挑针竖直向上复位。

Description

一种挑针自动复位机构及自动菌落挑选仪 技术领域
本发明涉及菌落挑选设备领域，尤其是涉及一种挑针自动复位机构及自动菌落挑选仪。
 背景技术
国内大规模基因测序、生物芯片、生物制药、免疫检测及化学合成等领域有许多工序急需用机器人代替繁琐的手工工作来确保高通量、高精度的流水线生产需要。基因测序工作中，通常需要用到自动菌落挑选仪将含有外源基因插入的宿主细胞挑取到液体培养基中。
相关技术中，自动菌落挑选仪包括呈闭环间隔均布的若干挑针，若干挑针共同连接于同一用于带动其绕闭环转动的传动机构，自动菌落挑选仪还包括用于驱动挑针竖直向下移动对目标菌落进行挑取和接种的驱动件，传动机构带动挑针转动，驱动件驱动挑针向下移动对菌落进行挑取或接种。自动菌落挑选仪还包括用于使挑针竖直向上复位的自动复位机构。
自动复位机构包括用于与传动机构固定的连接板，连接板上沿竖直方向滑移连接有用于固定挑针的固定板，连接板和固定板均沿传动机构的周向延伸，连接板与固定板之间设置有用于使固定板带动挑针竖直向上复位的弹簧。
相关技术中的连接板和固定板均沿传动机构的周向延伸，这样的设置方式使得自动复位机构相对于传动机构周向的体积占比较大，限制了相同空间内自动复位机构设置的最大组数，进而限制了自动菌落挑选仪的工作效率，因此存在一定的改进空间。
 技术问题
为了减小挑针自动复位机构的体积，本申请提供一种挑针自动复位机构及自动菌落挑选仪。
 技术解决方案
本申请提供的一种挑针自动复位机构及自动菌落挑选仪采用如下的技术方案：
一种挑针自动复位机构，包括用于与传动机构固定的连接件，所述连接件上竖向滑移连接有挑针固定件，所述挑针固定件用于固定挑针，所述挑针自动复位机构还包括弹性复位件，所述弹性复位件的两端分别连接于连接件和挑针固定件，所述挑针固定件上设置有用于动力源抵接施力的受力部，所述弹性复位件用于使挑针固定件带动挑针竖直向上复位。
通过采用上述技术方案，动力源对挑针固定件上的受力部施力，使挑针固定件带动挑针竖直向下运动，对目标菌落进行挑取和接种，完成目标菌落的挑取和接种后，挑针固定件在弹性复位件的作用下竖直向上复位，在实现挑针的自动复位的基础上，减小挑针自动复位机构的体积、简化挑针自动复位机构的结构，减小了挑针自动复位机构相对于传动机构周向的体积占比，增加了沿传动机构周向可设置的挑针自动复位机构的最大组数，进而提升了自动菌落挑选仪的工作效率。
优选的，所述挑针自动复位机构还包括限位部，所述限位部位于挑针固定件的移动路径上，用于限制所述挑针固定件向上移动的范围，所述挑针固定件上固设有用于与限位部抵接的抵接件，所述抵接件与限位部抵接时，所述弹性复位件处于受力状态。
通过采用上述技术方案，挑针固定件带动挑针竖直向上移动的过程中，挑针固定件移动至与限位部抵接，限制挑针固定件及挑针继续向上移动，确定挑针的复位位置，保证挑针的复位精度。
优选的，所述连接件为沿竖直方向穿设固定于传动机构的套件，所述挑针固定件包括与连接件同轴设置的导向柱，所述导向柱滑移穿设于连接件，所述挑针固设于导向柱底端，所述弹性复位件设置为同轴套设于导向柱外侧的压簧，所述导向柱顶端固设有上止动环，所述压簧顶端抵接于上止动环，所述压簧底端抵接于连接件，所述抵接件为固设于导向柱底端的下止动环。
通过采用上述技术方案，动力源对导向柱的顶端端面施加作用力，使导向柱带动挑针竖直向下移动，实现菌落的挑取或接种，挑针完成菌落的挑取或接种后，导向柱在压簧的作用下竖直向上复位，当下止动环移动至与连接件底端抵接时，导向柱及挑针停止移动，限制挑针的移动行程，确定挑针的复位位置，保证挑针的复位精度；同时，通过导向柱与连接件的配合，保证了导向柱移动过程中的垂直度，进而保证了挑针运动过程中的垂直度及重复定位精度。
优选的，所述连接件设置为直线轴承，所述导向柱与直线轴承配合。
通过采用上述技术方案，通过将连接件设置为直线轴承这一标准件，保证了导向柱移动过程中的垂直度的同时，降低了挑针自动复位机构的加工成本，有利于挑针自动复位机构的大批量生产。
优选的，所述导向柱底端开设有与其同轴的连接孔，所述连接孔与挑针相适配，所述挑针顶端插入固定于连接孔中。
通过采用上述技术方案，进一步提升了挑针的垂直度。
优选的，所述连接件包括用于与传动机构固定的连接板和固定于连接板的固定板，所述连接板沿垂直于传动机构的运动轨迹方向的切线方向设置，所述挑针固定件包括竖直设置的导轨，所述导轨滑移连接于连接件，所述导轨上固设有用于与挑针固定的连接条，所述弹性复位件设置为拉簧，所述拉簧顶端连接于连接件，所述拉簧底端连接于连接条，所述限位部包括固设于连接件的止动条，所述抵接件为固设于连接条的拉动杆，所述拉动杆位于止动条下方。
通过采用上述技术方案，动力源对导轨的顶端端面施加作用力，使导轨和连接条带动挑针竖直向下移动，实现菌落的挑取或接种；挑针完成菌落的挑取或接种后，导轨和连接条在拉簧的作用下竖直向上复位，当止动杆移动至与止动条抵接时，导轨、连接条及挑针停止移动，限制挑针的移动行程，确定挑针的复位位置，保证挑针的复位精度。
优选的，所述连接件上固设有与拉簧顶端的位置相对应的固定杆，所述拉簧顶端与固定杆连接，所述拉簧底端与拉动杆连接。
通过采用上述技术方案，拉动杆起到了与止动条配合的作用的同时，实现了拉簧底端与连接条之间的连接作用。
优选的，所述拉簧对称设置有两个。
通过采用上述技术方案，提升了对连接条及导轨的施力的稳定程度，减少了导轨的偏移，提升了导轨移动过程中的垂直度，进而保证了挑针运动过程中的垂直度及重复定位精度。
一种自动菌落挑选仪，包括传动机构，所述传动机构包括呈闭环状设置的传动件和用于为传动件提供动力的动力件，所述自动菌落挑选仪还包括若干挑针自动复位机构，若干所述挑针自动复位机构沿传动件周向间隔均布，所述连接件固设于传动件，所述挑针固定件底端固设有挑针，所述挑针竖向设置；
所述自动菌落挑选仪还包括沿传动件的运动轨迹方向依次排布的挑取工位、接种工位和清理工位，所述自动菌落挑选仪还包括与接种工位和挑取工位相对应的挑取工位驱动件和接种工位驱动件，所述挑取工位驱动件和接种工位驱动件用于提供挑针竖直向下移动的动力源。
 附图说明
图1是实施例一中显示自动菌落挑选仪的整体结构示意图。
图2是实施例一中显示挑针自动复位机构结构的局部结构示意图。
图3是实施例一中显示挑针自动复位机构结构的局部结构示意图。
图4是实施例一中显示清洗机构的局部结构示意图。
图5是实施例二中显示自动菌落挑选仪的整体结构示意图。
图6是实施例二中显示挑针自动复位机构结构的局部结构示意图。
图7是实施例三中显示清理工位的局部结构示意图。
附图标记说明：
1、工作台；11、转动盘；12、支撑架；13、相机；2、挑针；3、挑取工位；31、培养皿二维驱动机构；311、下线性模组；312、上线性模组；32、培养皿托板；4、接种工位；41、微孔板二维驱动机构；42、微孔板托板；5、清理工位；511、支架；5111、内凹槽；512、挡板；513、第一毛刷；514、第一酒精喷嘴；515、酒精导流管；521、热风枪；531、冷风枪；541、清洗台；542、第二毛刷；543、第二酒精喷嘴；551、加热台；552、加热通道；553、加热片；561、冷却台；562、冷却通道；563、冷却器喷嘴；61、挑取工位驱动件；62、接种工位驱动件；71、直线轴承；72、导向柱；721、上止动环；722、下止动环；723、连接孔；73、压簧；81、连接板；82、固定板；83、导轨；84、连接条；85、固定杆；86、止动杆；87、拉动杆；88、拉簧；89、止动条。
具体实施方式
以下结合附图1-7对本申请作进一步详细说明。
实施例一
本申请实施例一公开一种挑针自动复位机构及自动菌落挑选仪，参照图1，包括工作台1和设置于工作台1的控制器，工作台1上还转动连接有传动机构，传动机构包括传动件和用于驱动传动件绕自身周向转动的动力件，动力件与控制器电性连接。本实施例中，传动件为呈圆盘状设置的转动盘11，动力件为固定于工作台1且与转动盘11同轴设置的电机，转动盘11固定于电机的输出端。在其他实施例中，传动件还可以为其他呈闭环状设置的构件，动力件也可以根据传动件的设置进行选择，例如，当传动件为呈长圆型设置的同步带时，动力件可以设置为沿同步带的运动轨迹方向间隔设置于工作台1的同步带轮和用于驱动同步带转动的电机。
转动盘11的外缘处沿其周向间隔均布有若干组挑针自动复位机构，任意一组挑针自动复位机构底端均固定有用于进行菌落的挑取和接种的挑针2，挑针2竖向设置。
工作台1上沿转动盘11的运动轨迹方向依次设置有挑取工位3、接种工位4和清理工位5，挑取工位3、接种工位4和清理工位5分别用于供挑针2对菌落进行挑取、接种以及对挑针2进行清理。工作台1上固定有支撑架12，支撑架12上固定有与挑取工位3相对应的挑取工位驱动件61和与接种工位4相对应的接种工位驱动件62，挑取工位驱动件61和接种工位驱动件62分别用于驱动与挑取工位3和接种工位4相对应的挑针2竖直向下运动，实现菌落的挑取和接种。
结合图1和图2，挑针自动复位机构包括用于与转动盘11连接的连接件、沿竖直方向滑移连接于连接件的挑针固定件、用于使挑针固定件竖直向上复位的弹性复位件和用于限制挑针2向上移动的范围的限位部。
连接件为竖向设置的直线轴承71，本实施例中，直线轴承71设置为H型法兰直线轴承71。转动盘11上开设有与直线轴承71相适配的容纳孔，直线轴承71底端穿过容纳孔，直线轴承71顶端固定于转动盘11的上表面，直线轴承71的底端为本实施例中挑针自动复位机构中的限位部。挑针固定件包括竖向设置的导向柱72，导向柱72的横截面为圆形，导向柱72与直线轴承71沿竖向滑动配合。导向柱72顶端同轴固定有上止动环721，上止动环721的上表面与导向柱72的顶端端面平齐。弹性复位件为同轴套设于导向柱72外侧的压簧73，压簧73顶端抵接于上止动环721的下表面，压簧73底端抵接于直线轴承71的上表面。导向柱72底端的外周面上同轴固定有下止动环722，下止动环722的下表面与导向柱72的底端端面之间留有距离，下止动环722为本实施例中挑针自动复位机构中的抵接件。导向柱72在压簧73的作用下带动挑针2竖直向上复位，下止动环722移动至与直线轴承71的底端抵接时，压簧73处于压缩状态，此时挑针2处于完全复位状态，确定挑针2的复位位置，保证挑针2的复位精度；在实现挑针2自动复位的基础上，使挑针自动复位机构的整体结构小型化，进而减小了挑针自动复位机构于设备上的体积占比；同时，通过导向柱72与直线轴承71的配合，保证了导向柱72移动过程中的垂直度，进而保证了挑针2运动过程中的垂直度及重复定位精度。
参照图3，导向柱72底端开设有与其同轴设置的连接孔723，挑针2顶端插入固定于连接孔723中。
参照图1，挑取工位3用于对培养皿进行水平面内的二维控制，挑取工位3包括培养皿二维驱动机构31和用于承托培养皿的培养皿托板32。培养皿二维驱动机构31包括下线性模组311和连接于下线性模组311的输出端的上线性模组312，培养皿托板32固定连接于上线性模组312的输出端，上线性模组312的输送方向与下线性模组311的输送方向相互垂直，上线性模组312和下线性模组311均与控制器电性连接，控制器控制上线性模组312和下线性模组311的输出端移动，实现对培养皿位置的调整。
支撑架12上还设置有相机13，相机13与控制器电性连接，相机13用于对培养皿测距并拍照后将照片传至图像处理软件，带有菌落的图像经软件处理后提取出目标菌落的坐标信息，进而控制挑取工位3驱动培养皿移动至培养皿上的目标菌落与挑针2的位置相对应。
本实施例中，挑取工位驱动件61为竖向设置的电缸，电缸与控制器电性连接，电缸的杆端向下移动至与导向柱72的顶端端面抵接，对导向柱72施加作用力，使导向柱72带动挑针2竖直向下移动，实现对挑针2的驱动。其他实施例中，挑取工位驱动件61还可以设置为其他能进行行程的动态调整的执行机构。菌落挑取的过程中，为了取得最佳的实验效果，实验人员需要仅针对菌落表面实施挑取作业，但是由于每次所做的培养皿中的培养基厚度不同，导致菌落表面距离挑针2的高度是变化的，通过电缸将相机13识别的菌落的动态高度转化为动态行程，实现对挑针2的每次挑取行程的动态调整，进而实现对菌落挑取的高度的控制，进一步提高了实验结果的科学性和可靠性。
接种工位4用于对微孔板进行水平面内的二维控制，接种工位4包括微孔板二维驱动机构41和用于承托微孔板的微孔板托板42，微孔板托板42与微孔板二维驱动机构41的输出端连接，微孔板二维驱动机构41与控制器电性连接。接种工位4中的微孔板二维驱动机构41与挑取工位3中的培养皿二维驱动机构31的结构相同，在此不再赘述。
本实施例中，接种工位驱动件62为竖向设置的气缸，气缸与控制器电性连接，气缸的杆端向下移动至与导向柱72的顶端端面抵接，对导向柱72施加作用力，使导向柱72带动挑针2竖直向下移动，实现对挑针2的驱动。在其他实施例中，接种工位驱动件62还可以设置为电缸或其他能进行直线往复运动的执行机构。
结合图1和图4，清理工位5包括清洗机构、加热机构和冷却机构，清洗机构、加热机构和冷却机构沿转动盘11的运动轨迹方向依次排布。
清洗机构包括固定于工作台1的支架511，支架511顶端设置有挡板512，挡板512沿转动盘11的径向间隔设置有两块，两块挡板512相对的两侧壁之间形成用于供挑针2通过的清洗通道。挡板512沿转动盘11的径向滑移连接于支架511，支架511上开设有长圆型孔，长圆型孔中自下而上穿设有锁紧螺栓，锁紧螺栓螺纹连接于挡板512底端，锁紧螺栓用于将挡板512锁紧于固定位置。清洗通道的两侧壁上分别固定有第一毛刷513，第一毛刷513的刷毛水平设置，第一毛刷513于清洗通道的同侧间隔设置有两组。位于外侧的挡板512上安装固定有第一酒精喷嘴514，第一酒精喷嘴514位于两组第一毛刷513中间，第一酒精喷嘴514沿转动盘11的径向设置。支架511的顶端端面于两组第一毛刷513中间的位置向下凹陷形成呈斗状设置的内凹槽5111，支架511底端安装有酒精导流管515，酒精导流管515顶端与内凹槽5111的内腔连通。
挑针2随转动盘11转动时，挑针2进入于消毒通道中，第一毛刷513将挑针2上大块的残留物刷掉，同时第一酒精喷嘴514向外喷出酒精对挑针2进行消毒，此时第一毛刷513还起到了减少酒精喷溅的作用。另外，酒精通过内凹槽5111和酒精导流管515排出。
加热机构设置为热风枪521，热风枪521的喷头竖直向上设置，热风枪521用于对经过其上方的挑针2喷出热风将挑针2烘干。
冷却机构设置为冷风枪531，冷风枪531的喷头竖直向上设置，用于对经过其上方的挑针2喷出冷风使挑针2降温。
本申请实施例一种挑针自动复位机构及自动菌落挑选仪的实施原理为：
自动菌落挑选仪工作的过程中，转动盘11在电机的作用下转动，带动挑针自动复位机构以及挑针2转动，转动过程中，挑针2转动至清洗机构、加热机构和冷却机构中完成清洗消毒、加热和冷却工作，实现对挑针2的清理；
转动盘11每转动一个挑针间距，不同挑针2分别转动至挑取工位驱动件61和接种工位驱动件62正下方，控制器控制转动盘11停顿，培养皿二维驱动机构31和微孔板二维驱动机构41分别将培养皿和微孔板移动至挑针2的正下方，挑取工位驱动件61和接种工位驱动件62分别对相应的导向柱72的顶端端面施加作用力，使相应的挑针2竖直向下运动实现对菌落的挑取和接种；
挑针2完成菌落的挑取或接种后，导向柱72在压簧73的作用下竖直向上复位，当下止动环722移动至与直线轴承71的底端端面抵接时，导向柱72及挑针2停止移动，确定挑针2的复位位置；
重复上述步骤直至所有菌落都被接种到微孔板中。
实施例二
结合图5和图6，一种挑针自动复位机构及自动菌落挑选仪，与实施例一不同之处在于，连接件包括呈L型设置的连接板81，连接板81水平设置的一端沿转动盘11的径向延伸且使用螺栓压紧固定于转动盘11的上表面，连接板81竖直设置的一端远离转动盘11的轴线的侧壁上使用螺栓压紧连接有固定板82，固定板82位于转动盘11外侧。挑针固定件包括滑移连接于固定板82远离连接板81的侧壁的导轨83，导轨83竖向设置。导轨83远离固定板82的一侧使用螺栓压紧连接有竖向设置的连接条84，挑针2固定于连接条84底端。
用于连接固定板82和连接板81的螺栓呈矩形阵列设置有四个，四个螺栓两两分布于导轨83的两侧，四个螺栓的杆部均伸出于连接条84远离导轨83的侧壁。位于上方的两个螺栓的杆部设置为固定杆85，位于下方的两个螺栓的杆部设置为止动杆86，且两个固定杆85位于同一高度，两个止动杆86位于同一高度。
用于连接导轨83和连接条84的螺栓上下间隔设置有两个，位于下方的螺栓的杆部伸出于连接条84远离导轨83的侧壁，位于下方的螺栓的杆部设置为拉动杆87，拉动杆87位于止动杆86下方，两个固定杆85关于拉动杆87对称设置。
弹性复位件设置为拉簧88，两个拉簧88的一端套设于拉动杆87，两个拉簧88的另一端分别套设于两个固定杆85外侧。
两个止动杆86上共同连接有同一止动条89，止动条89水平设置且与两个止动杆86垂直，止动条89的两端分别套设固定于两个止动杆86，止动条89和两个止动杆86共同本实施例中的限位部。
挑针2在拉簧88的作用下向上移动至拉动杆87与止动杆86底端抵接时，拉簧88处于拉伸状态，挑针2无法继续向上移动，确定了挑针2的复位位置。
上述实施例的实施原理为：
挑取工位驱动件61或接种工位驱动件62分别对相应的导轨83的顶端端面施加作用力，使相应的导轨83和连接条84分别带动对应的挑针2竖直向下移动，实现菌落的挑取或接种；挑针2完成菌落的挑取或接种后，导轨83和连接条84在拉簧88的作用下竖直向上复位，当止动杆86移动至与止动条89抵接时，导轨83、连接条84及挑针2停止移动，确定挑针2的复位位置，保证挑针2的复位精度，在实现挑针2的自动复位的基础上，使挑针复位机构的整体结构小型化，进而减小了挑针自动复位机构于设备上的体积占比。
实施例三
结合图6和图7，一种挑针自动复位机构及自动菌落挑选仪，与实施例一不同之处在于， 清洗机构包括清洗台541，清洗台541包括两块沿转动盘11的径向间隔设置的弧形板，弧形板与转动盘11同轴设置，两个弧形板相对的两侧壁之间形成供挑针2通过的清洗通道。清洗通道的两侧壁上分别固定有第二毛刷542，第二毛刷542的刷毛水平设置，第二毛刷542于清洗通道的同侧间隔设置有两组，两组第二毛刷542中间安装固定有安装固定有第二酒精喷嘴543。
加热机构包括固定于工作台1的加热台551，加热台551的机构与清洗台541的结构相同，加热台551中设置有加热通道552。加热通道552的两侧壁上分别固定有加热片553，加热片553沿加热通道552的延伸方向延伸，本实施例中，加热片553设置为陶瓷加热片553。
冷却机构包括固定于工作台1的冷却台561，冷却台561的机构与清洗台541的结构相同，冷却台561中设置有冷却通道562。冷却通道562的两侧壁上分别固定有冷却器喷嘴563，冷却器喷嘴563沿转动盘11的径向设置，冷却器喷嘴563沿冷却通道562的延伸方向间隔均布有若干。
上述实施例的实施原理为：
挑针2随转动盘11转动时，挑针2进入于消毒通道中，第二毛刷542将挑针2上大块的残留物刷掉，同时第二酒精喷嘴543向外喷出酒精对挑针2进行清洗及消毒，此时第二毛刷542还起到了减少酒精喷溅的作用，挑针2继续移动至加热通道552中，加热片553释放热量将挑针2烘干，挑针2继续移动至冷却通道562中，冷却器喷嘴563对挑针2进行降温，在挑针2无需上下移动的前提下，实现了对挑针2的清洗消毒、加热及冷却，延长了挑针2的清理时间，减少了清理不彻底的因素，提高了实验结果的可靠性和科学性。
以上均为本申请的较佳实施例，并非依此限制本申请的保护范围，故：凡依本申请的结构、形状、原理所做的等效变化，均应涵盖于本申请的保护范围之内。

Claims (9)

  1. 一种挑针自动复位机构，其特征在于：包括用于与传动机构固定的连接件，所述连接件上竖向滑移连接有挑针固定件，所述挑针固定件用于固定挑针(2)，所述挑针自动复位机构还包括弹性复位件，所述弹性复位件的两端分别连接于连接件和挑针固定件，所述挑针固定件上设置有用于动力源抵接施力的受力部，所述弹性复位件用于使挑针固定件带动挑针(2)竖直向上复位。
  2. 根据权利要求1所述的一种挑针自动复位机构，其特征在于：所述挑针自动复位机构还包括限位部，所述限位部位于挑针固定件的移动路径上，用于限制所述挑针固定件向上移动的范围，所述挑针固定件上固设有用于与限位部抵接的抵接件，所述抵接件与限位部抵接时，所述弹性复位件处于受力状态。
  3. 根据权利要求2所述的一种挑针自动复位机构，其特征在于：所述连接件为沿竖直方向穿设固定于传动机构的套件，所述挑针固定件包括与连接件同轴设置的导向柱(72)，所述导向柱(72)滑移穿设于连接件，所述挑针(2)固设于导向柱(72)底端，所述弹性复位件设置为同轴套设于导向柱(72)外侧的压簧(73)，所述导向柱(72)顶端固设有上止动环(721)，所述压簧(73)顶端抵接于上止动环(721)，所述压簧(73)底端抵接于连接件，所述抵接件为固设于导向柱(72)底端的下止动环(722)。
  4. 根据权利要求3所述的一种挑针自动复位机构，其特征在于：所述连接件设置为直线轴承(71)，所述导向柱(72)与直线轴承(71)配合。
  5. 根据权利要求3所述的一种挑针自动复位机构，其特征在于：所述导向柱(72)底端开设有与其同轴的连接孔(723)，所述连接孔(723)与挑针(2)相适配，所述挑针(2)顶端插入固定于连接孔(723)中。
  6. 根据权利要求2所述的一种挑针自动复位机构，其特征在于：所述连接件包括用于与传动机构固定的连接板(81)和固定于连接板(81)的固定板(82)，所述连接板(81)沿垂直于传动机构的运动轨迹方向的切线方向设置，所述挑针固定件包括竖直设置的导轨(83)，所述导轨(83)滑移连接于连接件，所述导轨(83)上固设有用于与挑针(2)固定的连接条(84)，所述弹性复位件设置为拉簧(88)，所述拉簧(88)顶端连接于连接件，所述拉簧(88)底端连接于连接条(84)，所述限位部包括固设于连接件的止动条(89)，所述抵接件为固设于连接条(84)的拉动杆(87)，所述拉动杆(87)位于止动条(89)下方。
  7. 根据权利要求6所述的一种挑针自动复位机构，其特征在于：所述连接件上固设有与拉簧(88)顶端的位置相对应的固定杆(85)，所述拉簧(88)顶端与固定杆(85)连接，所述拉簧(88)底端与拉动杆(87)连接。
  8. 根据权利要求7所述的一种挑针自动复位机构，其特征在于：所述拉簧(88)对称设置有两个。
  9. 一种自动菌落挑选仪，其特征在于：包括传动机构，所述传动机构包括呈闭环状设置的传动件和用于为传动件提供动力的动力件，所述自动菌落挑选仪还包括若干如权利要求1-8中任一所述的挑针自动复位机构，若干所述挑针自动复位机构沿传动件周向间隔均布，所述连接件固设于传动件，所述挑针固定件底端固设有挑针(2)，所述挑针(2)竖向设置；
    所述自动菌落挑选仪还包括沿传动件的运动轨迹方向依次排布的挑取工位(3)、接种工位(4)和清理工位(5)，所述自动菌落挑选仪还包括与接种工位(4)和挑取工位(3)相对应的挑取工位驱动件和接种工位驱动件(62)，所述挑取工位驱动件(61)和接种工位驱动件(62)用于提供挑针(2)竖直向下移动的动力源。
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CN113604348A (zh) * 2021-07-26 2021-11-05 中国科学院深圳先进技术研究院 自动菌落挑选仪及挑选方法
CN113667588A (zh) * 2021-07-26 2021-11-19 中国科学院深圳先进技术研究院 一种挑针自动复位机构及自动菌落挑选仪

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