WO2023004614A1

WO2023004614A1 - Method and system for correcting current signal

Info

Publication number: WO2023004614A1
Application number: PCT/CN2021/108837
Authority: WO
Inventors: 杨稳; 沈钢
Original assignee: 三诺生物传感股份有限公司
Priority date: 2021-07-28
Filing date: 2021-07-28
Publication date: 2023-02-02

Abstract

Disclosed are a method and system for correcting a current signal. The method comprises: calculating n slopes of a current signal curve in an effective current interval, wherein n≥2; obtaining a compensation factor through calculation by means of the n slopes; and correcting a current signal on the basis of the compensation factor to obtain a corrected current signal. According to the present invention, during electrochemical detection, the current signal may be effectively corrected to reduce or eliminate the influence of temperature on a test result, thereby improving the accuracy of the test result.

Description

Method and system for correcting current signal

technical field

The invention relates to the technical field of electrochemical detection, in particular to a method and system for correcting current signals.

Background technique

At present, in electrochemical detection, there is a problem that the detection result is inaccurate due to the influence of temperature. For example, the measurement of blood glucose concentration realized by electrochemical principles mainly collects the current in the reaction area to reflect the concentration information of glucose in the blood. The greater the current, the higher the glucose concentration, and vice versa; The current signal is greatly affected by temperature, the higher the temperature, the faster the chemical reaction and electron migration, the greater the current; conversely, the lower the temperature, the slower the chemical reaction and electron migration, the smaller the current; therefore , in order to make the accuracy and precision of the test results better, the test process needs to add temperature correction to the current signal to reduce or eliminate the influence of temperature on the test results.

At present, the temperature correction methods for current signals are mainly divided into the following two types:

First, add a heat-sensitive sensing component to the instrument to directly measure the ambient temperature of the heat-sensitive component inside the instrument, and use the temperature coefficient for current correction to reduce the influence of temperature on the test value; but in some Under the environmental background (environmental temperature changes greatly), the internal temperature of the instrument is inconsistent with the temperature of the reaction zone on the test strip, which leads to over-correction and inaccurate results; therefore, it is necessary to use the internal temperature of the instrument instead of the temperature of the reaction zone to correct the test result value. limitation.

Second, special materials are used in the production of test strips to make the electrodes of the test strips have temperature characteristics, so that the temperature information of the reaction zone can be obtained for the correction of the result values; however, this kind of test strips has extremely strict requirements on the manufacturing process. The requirements for consistency, accuracy and precision are high; the method is therefore currently limited in terms of production.

Therefore, in the electrochemical detection, how to effectively correct the current signal to reduce or eliminate the influence of temperature on the test results, and thus improve the accuracy of the test results, is an urgent problem to be solved.

Contents of the invention

In view of this, the present invention provides a method for correcting the current signal, which can effectively correct the current signal during electrochemical detection, reduce or eliminate the influence of temperature on the test result, and further improve the accuracy of the test result.

The invention provides a method for correcting a current signal, comprising:

Calculate n slopes of the current signal curve in the effective current range, where n≥2;

Compensation factors are obtained by calculating the n slopes;

The current signal is corrected based on the compensation factor to obtain a corrected current signal.

Preferably, the calculation of n groups of slopes in the effective current interval of the current signal curve, where n≥2, includes:

In the effective current interval, select a plurality of data points on the current signal curve, perform two pairs of two data points in the plurality of data points respectively, determine n pairs of data, and use each Sorting the n pairs of data in chronological order based on the time node where the data points earlier in the time are located in the data, wherein at least one data point is different in every two pairs of data in the n pairs of data;

Based on the slope formula, the slopes of the n pairs of data are calculated to obtain n slopes Kn, wherein the n slopes Kn have the same sorting sequence as the n pairs of data.

Preferably, the compensation factor calculated through the n slopes includes:

Based on the sorting sequence of the n slopes Kn, respectively substitute two adjacent slopes into the slope equation, and calculate n-1 time values tn-1;

The obtained n-1 time values tn-1 are averaged to obtain the compensation factor t.

Preferably, the correcting the current signal based on the compensation factor to obtain the corrected current signal includes:

Based on the compensation factor t, a corrected current signal I _new is obtained according to the formula I _new =I _old +t*slope+intercept, wherein I _old is a current value at a predetermined time point, and slope and intercept are known values.

Preferably, the current value at the predetermined time point is a current value corresponding to a current signal preceding the last current signal among all the acquired current signals.

A system for correcting a current signal comprising:

The first calculation module is used to calculate n slopes of the current signal curve in the effective current range, where n≥2;

The second calculation module is used to obtain the compensation factor through the calculation of the n slopes;

A correction module, configured to correct the current signal based on the compensation factor to obtain a corrected current signal.

Preferably, the first calculation module is specifically used for:

Preferably, the second calculation module is specifically used for:

Preferably, the correction module is specifically used for:

In summary, the present invention discloses a method for correcting the current signal. When the current signal needs to be corrected, first calculate the n slopes of the current signal curve in the effective current range, where n≥2, and then pass n The compensation factor is obtained by calculating the slope, and the current signal is corrected based on the compensation factor to obtain the corrected current signal. The invention can correct the current signal through the calculated compensation factor, so as to reduce or eliminate the influence of temperature on the electrochemical detection test result, thereby improving the accuracy of the test result.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention. Those skilled in the art can also obtain other drawings based on these drawings without creative work.

Fig. 1 is a method flow chart of an embodiment of a method for correcting a current signal disclosed in the present invention;

Fig. 2 is a schematic diagram of calculating the two slopes of the initial current signal curve in the effective current interval disclosed by the present invention;

Fig. 3 is a schematic diagram of calculating the time value through two sets of slopes disclosed by the present invention;

Fig. 4 is a schematic diagram of a blood glucose current signal after correction and compensation disclosed by the present invention;

Fig. 5 is a schematic structural diagram of an embodiment of a system for correcting current signals disclosed in the present invention.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

As shown in Figure 1, it is a flow chart of an embodiment of a method for correcting a current signal disclosed in the present invention, and the method may include the following steps:

S101. Calculate n slopes of the initial current signal curve in the effective current range, where n≥2;

In the process of electrochemical detection, when the current signal needs to be corrected, firstly, two or more slopes of the initial current signal curve in the effective current range are calculated.

Among them, the effective current interval is the interval determined by the research and development personnel based on experience according to different detection items (mainly the influence of temperature on the reaction speed). Generally, it is determined that the last 1-2 seconds when the detection instrument can obtain the signal is taken as the effective current interval. For example, for a blood glucose test, the entire signal acquisition period is 5 seconds, and developers generally use the 3rd to 5th second as the effective current interval.

Since there are many abnormal values in the current signal in the non-effective current interval, the accuracy of the calculation can be effectively improved by setting the data in the effective current interval, and then the accuracy of the current signal correction can be improved.

S102. Compensation factors are obtained by calculating n slopes;

The length of time for chemical reactions to reach equilibrium can be considered as the length of time for the current curve to go from steep to flat in the current signal curve, and the length of the equilibrium time has temperature characteristics and concentration characteristics. Therefore, the length of equilibrium time can be used as compensation factor to correct the effect of temperature on the current signal.

Therefore, after two or more slopes are calculated, the compensation factor is further calculated through two or more slopes.

S103. Correct the current signal based on the compensation factor to obtain a corrected current signal.

After the compensation factor is calculated, an equation is established to compensate the current signal according to the actual situation, which is used to correct the influence of temperature on the current signal. That is, the current signal is corrected according to the obtained compensation factor to obtain the corrected current signal.

To sum up, in the above embodiment, when the current signal needs to be corrected, first calculate the n slopes of the initial current signal curve in the effective current range, where n≥2, and then calculate the compensation by n slopes factor, the current signal is corrected based on the compensation factor, and the corrected current signal is obtained. The current signal can be corrected by the calculated compensation factor to reduce or eliminate the influence of temperature on the electrochemical detection test result, thereby improving the accuracy of the test result.

Specifically, in the above embodiment, one of the implementations of calculating the n slopes of the initial current signal curve in the effective current interval may be:

First, in the effective current interval, select a plurality of data points on the initial current signal curve, use two data points in the plurality of data points to make two pairs of pairs, determine n pairs of data, and use each pair of data Sort n pairs of data in chronological order based on the time node where the data points earlier in time are located, where at least one data point is different in every two pairs of data in n pairs of data; for example, when n slopes need to be calculated , the number of data points to be selected can be 2n, or when some data points are shared, the number of data points to be selected can be n+1. It should be noted that the time interval between the two data points used in the calculation of each slope should be similar, preferably with the same time interval; in addition, the two data points used in the calculation of the same slope The time interval between points should be as large as possible; combined with the above two selection requirements for data points, n pairs of data can be easily determined within the effective current interval. For the data points that meet the above two selection requirements, the calculated slope is more accurate.

Then, based on the slope formula, the slopes of the n pairs of data are calculated to obtain n slopes Kn, wherein the n slopes Kn have the same sorting sequence as the n pairs of data.

Specifically, the calculation of the two slopes of the initial current signal curve in the effective current range is taken as an example below, as shown in Figure 2:

formula based

Calculate the first slope K1, where Ia1 is the first current value corresponding to point a1 on the initial current signal curve, Ta1 is the first time corresponding to point a1, and Ia2 is the first current value corresponding to point a2 on the initial current signal curve. The second current value, Ta2 is the second time corresponding to the point a2;

formula based

Calculating the second slope K2, wherein, Ib1 is the third current value corresponding to point b1 on the initial current signal curve, Tb1 is the third time corresponding to the point b1, and Ib2 is corresponding to point b2 on the initial current signal curve The fourth current value of Tb2 is the fourth time corresponding to the point b2.

Specifically, in the above embodiment, another way to calculate the two slopes of the initial current signal curve in the effective current range may be:

formula based

Calculate the first slope K1, where D is the scaling factor, I is the offset, Ia1 is the first current value corresponding to point a1 on the initial current signal curve, Ta1 is the first time corresponding to the point a1, Ia2 is the second current value corresponding to point a2 on the initial current signal curve, and Ta2 is the second time corresponding to point a2; wherein, the scaling factor D and the offset I can be adjusted according to actual needs, by adjusting the scaling factor The adjustment of D and the offset I can make the calculated first slope K1 more optimal.

formula based

Calculate the second slope K2, wherein D is a scaling factor, I is an offset, Ib1 is a third current value corresponding to point b1 on the initial current signal curve, Tb1 is a third time corresponding to point b1, and Ib2 is the fourth current value corresponding to the point b2 on the initial current signal curve, and Tb2 is the fourth time corresponding to the point b2. Wherein, the scaling factor D and the offset I can be adjusted according to actual needs, and the calculated second slope K2 can be made more optimal by adjusting the scaling factor D and the offset I.

Specifically, in the above embodiment, one of the implementations of obtaining the compensation factor by calculating n slopes may be:

First, based on the sorting sequence (K1, K2, K3...Kn) of n slopes Kn, respectively substitute two adjacent slopes into the slope equation

Calculate n-1 time values tn-1; for example, as shown in Figure 3, the slopes K1 and K2 are substituted into the formula

Let K=0, calculate t1, where Ta1, Ta2, Tb1, Tb2 are known values.

Then, the obtained n-1 time values tn-1 (t1, t2, t3...tn-1) are averaged to obtain the compensation factor t.

Specifically, in the above embodiment, the current signal is corrected based on the compensation factor, and one of the implementations for obtaining the corrected current signal may be:

Based on the compensation factor t, the corrected current signal I _new is obtained according to the formula I _new =I _old +t*slope+intercept. Wherein, the formula _Inew = _Iold +t*slope+intercept is a known function pre-existing in the instrument system, the slope slope and the intercept intercept in this function are known definite values, and t and _Iold are variables; Cooperate with the test strip to detect the current signal, obtain the current signal curve, calculate the compensation factor t, and select the current value I _old at a predetermined time point, and substitute t and I _old into the formula I _new = I _old + t*slope +intercept calculates the corrected current signal I _new , and the instrument calculates an accurate test value based on the corrected current signal I _new as the current signal after temperature correction.

Among them, in the factory stage of the instrument, the specific values of slope and intercept can be determined by means of linear function regression. Specifically, by using multiple training samples and testing at different temperatures, the current curve at abnormal temperature and the current curve at normal temperature can be obtained; using the same calculation method, the compensation factor t corresponding to each current curve Calculated, and at the same time node, the current value of normal temperature and the current value of abnormal temperature are corresponding, and the linear function regression is performed to determine the slope and intercept. Of course, the slope and intercept can also be directly determined by the experience of the R&D personnel.

Specifically, in the above-mentioned embodiment, regarding the current value I _old at a certain predetermined time point, in the selection of the time point, try to select all the current signals that can be detected by the instrument. The current signal of the current signal, choose a current value as I _old ; preferably the previous current signal value of the last current signal as I _old .

As shown in Figure 4, it is a schematic diagram of a corrected and compensated blood sugar current signal disclosed in an embodiment of the present invention. It can be seen from Figure 4 that the corrected blood sugar current signal is hardly affected by temperature, and is only related to blood sugar concentration, so According to the technical solution provided by the present invention, the temperature compensation and correction of the blood sugar current signal can be better realized, and the influence of temperature on the blood sugar test result can be reduced or eliminated, thereby improving the accuracy of the blood sugar test result.

As shown in FIG. 5, it is a schematic structural diagram of a system embodiment for correcting current signals disclosed in the present invention. The system may include:

The first calculation module 501 is used to calculate n slopes of the current signal curve in the effective current range, where n≥2;

The second calculation module 502 is used to obtain the compensation factor through n slope calculations;

The correction module 503 is configured to correct the current signal based on the compensation factor to obtain a corrected current signal.

The working principle of the system for correcting the current signal disclosed in this embodiment is the same as that of the above-mentioned embodiment of the method for correcting the current signal, and will not be repeated here.

Each embodiment in this specification is described in a progressive manner, each embodiment focuses on the difference from other embodiments, and the same and similar parts of each embodiment can be referred to each other. As for the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and for the related part, please refer to the description of the method part.

Professionals can further realize that the units and algorithm steps of the examples described in conjunction with the embodiments disclosed herein can be implemented by electronic hardware, computer software or a combination of the two. In order to clearly illustrate the possible For interchangeability, in the above description, the composition and steps of each example have been generally described according to their functions. Whether these functions are implemented by hardware or software depends on the specific application and design constraints of the technical solution. Those skilled in the art may use different methods to implement the described functions for each specific application, but such implementation should not be regarded as exceeding the scope of the present invention.

The steps of the methods or algorithms described in connection with the embodiments disclosed herein may be directly implemented by hardware, software modules executed by a processor, or a combination of both. Software modules can be placed in random access memory (RAM), internal memory, read-only memory (ROM), electrically programmable ROM, electrically erasable programmable ROM, registers, hard disk, removable disk, CD-ROM, or any other Any other known storage medium.

The above description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

A method for correcting a current signal, comprising:

Calculate n slopes of the current signal curve in the effective current range, where n≥2;

Compensation factors are obtained by calculating the n slopes;

The current signal is corrected based on the compensation factor to obtain a corrected current signal.
The method according to claim 1, wherein the calculation of n groups of slopes in the effective current interval of the current signal curve, where n≥2, includes:

In the effective current interval, select a plurality of data points on the current signal curve, perform two pairs of two data points in the plurality of data points respectively, determine n pairs of data, and use each Sorting the n pairs of data in chronological order based on the time node where the data points earlier in the time are located in the data, wherein at least one data point is different in every two pairs of data in the n pairs of data;

Based on the slope formula, the slopes of the n pairs of data are calculated to obtain n slopes Kn, wherein the n slopes Kn have the same sorting sequence as the n pairs of data.
The method according to claim 2, wherein said calculation of said n slopes to obtain compensation factors comprises:

Based on the sorting sequence of the n slopes Kn, respectively substitute two adjacent slopes into the slope equation, and calculate n-1 time values tn-1;

The obtained n-1 time values tn-1 are averaged to obtain the compensation factor t.
The method according to claim 3, wherein the correcting the current signal based on the compensation factor to obtain the corrected current signal comprises:

Based on the compensation factor t, a corrected current signal I new is obtained according to the formula I new =I old +t*slope+intercept, wherein I old is a current value at a predetermined time point, and slope and intercept are known values.
The method according to claim 4, wherein the current value at the predetermined time point is a current value corresponding to a current signal preceding the last current signal among all the acquired current signals.
A system for correcting current signals, characterized in that it comprises:

The first calculation module is used to calculate n slopes of the current signal curve in the effective current range, where n≥2;

The second calculation module is used to obtain the compensation factor through the calculation of the n slopes;

A correction module, configured to correct the current signal based on the compensation factor to obtain a corrected current signal.
The system according to claim 1, wherein the first calculation module is specifically used for:

In the effective current interval, select a plurality of data points on the current signal curve, perform two pairs of two data points in the plurality of data points respectively, determine n pairs of data, and use each Sorting the n pairs of data in chronological order based on the time node where the data points earlier in the time are located in the data, wherein at least one data point is different in every two pairs of data in the n pairs of data;

Based on the slope formula, the slopes of the n pairs of data are calculated to obtain n slopes Kn, wherein the n slopes Kn have the same sorting sequence as the n pairs of data.
The system according to claim 7, wherein the second calculation module is specifically used for:

Based on the sorting sequence of the n slopes Kn, respectively substitute two adjacent slopes into the slope equation, and calculate n-1 time values tn-1;

The obtained n-1 time values tn-1 are averaged to obtain the compensation factor t.
The system according to claim 8, wherein the correction module is specifically used for:

Based on the compensation factor t, a corrected current signal I new is obtained according to the formula I new =I old +t*slope+intercept, wherein I old is a current value at a predetermined time point, and slope and intercept are known values.
The system according to claim 9, wherein the current value at the predetermined time point is a current value corresponding to a current signal preceding the last current signal among all the acquired current signals.