WO2022245035A1 - Oral bacteria test stick - Google Patents

Oral bacteria test stick Download PDF

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WO2022245035A1
WO2022245035A1 PCT/KR2022/006653 KR2022006653W WO2022245035A1 WO 2022245035 A1 WO2022245035 A1 WO 2022245035A1 KR 2022006653 W KR2022006653 W KR 2022006653W WO 2022245035 A1 WO2022245035 A1 WO 2022245035A1
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oral
present
bacteria
bacteria test
oral bacteria
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PCT/KR2022/006653
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French (fr)
Korean (ko)
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고정욱
반용욱
임연철
김형진
정영의
이왕종
박서혜
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주식회사 핏펫
주식회사 미리메딕스
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Publication of WO2022245035A1 publication Critical patent/WO2022245035A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour

Definitions

  • the present invention relates to an oral bacteria test rod, and more particularly, to an oral bacteria test rod configured to detect specific enzymes produced by anaerobic bacteria that cause oral diseases.
  • Pets can have more difficult oral care than humans, making them more susceptible to bacteria that cause oral disease.
  • anaerobic bacteria in dental plaque can cause periodontal disease.
  • inflammation may occur in the gums. At this time, if the inflammation is not treated, it may progress to periodontitis. Eventually, the tooth becomes loose and falls out, or pus collects and cannot return to its original state.
  • X-ray imaging and a periodontal probe test using a periodontal probe there may be X-ray imaging and a periodontal probe test using a periodontal probe.
  • X-ray imaging and periodontal probe inspection are performed based on the degree of biofilm formation, calculus, and inflammation, they are used not for prevention purposes but for checking the progress after the occurrence of disease.
  • benzoyl-DL-arginine-a-naphthylamide (benzoyl-DL-arginine-a- naphthylamide) based diagnostic products appeared.
  • a conventional diagnostic product based on a chromophoric material consists of a cotton swab, a strip pad, and temperature control equipment. After soaking a cotton swab with the oral saliva of a companion animal, the cotton swab is dipped in the strip pad and inserted into the temperature control equipment. Color development may occur at °C. Then, based on the color development, it may be configured to confirm whether oral disease-causing bacteria are present.
  • a conventional diagnostic product based on a strip pad may require temperature-saving equipment that provides a specific temperature for color development, and there may be a limitation that a remarkably long time is required to confirm the reaction result.
  • the inventors of the present invention in order to overcome the limitations of the conventional companion animal oral disease diagnosis method, can not only detect enzymes specifically produced by anaerobic bacteria, which are the causative bacteria of periodontal disease, but also color expression at room temperature Attention was paid to the causative agent.
  • the inventors of the present invention have found a non-toxic material that is more reactive than conventional chromophoric materials without a temperature control device, enabling shortening of the reaction time, and produced it in the form of a strip pad or cotton swab.
  • the inventors of the present invention could expect that since the new oral bacteria test rod can express color even at room temperature, additional temperature control equipment is not required, thereby reducing the user's economic burden and increasing convenience of use.
  • an object of the present invention is to substantially supplement various problems caused by limitations and disadvantages in the prior art.
  • an object of the present invention is to provide an oral bacteria test rod capable of continuously monitoring the oral health of a companion animal.
  • the chromogenic substance reacts with a specific enzyme at room temperature to produce 4-nitroaniline to induce color expression.
  • the specific enzyme is Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tanerella spor It may include a trypsin like enzyme generated from at least one of Tannerella forsythia, Streptococcus mutans and Lactobacillus.
  • the chromogenic material is a tissue plasminogen activator (tPA) substrate or Na-benzoyl-L-arginine 4-nitroanilide hydrochloride (N ⁇ -Benzoyl- L-arginine 4-nitroanilide hydrochloride; BAPNA).
  • tPA tissue plasminogen activator
  • BAPNA Na-benzoyl-L-arginine 4-nitroanilide hydrochloride
  • the present invention provides an oral monitoring system that reacts specifically with oral disease-related bacteria to produce a chromogenic substance, a conventional oral disease diagnosis method based on X-ray imaging and / or probing, and furthermore, temperature control equipment is required. There is an effect that can overcome the limitations of conventional strip pad-based diagnostic products.
  • the present invention can provide an oral monitoring system based on a chromophoric substance that develops at room temperature, is more reactive than conventional chromophoric substances, can shorten reaction time, and is non-toxic.
  • the present invention can reduce the user's economic burden and increase the convenience of use because a separate temperature control device is not required because color expression is possible even at room temperature.
  • the present invention has an effect of preventing oral diseases such as periodontal disease by more simply checking the presence or absence of oral disease-causing bacteria.
  • the present invention can contribute to a good treatment prognosis because it is possible to diagnose oral diseases early, so that appropriate treatment can be performed on companion animals before they go to a severe stage.
  • FIG. 1 and 2 illustratively illustrates the configuration of an oral bacteria test rod according to another embodiment of the present invention.
  • FIG. 3 illustratively illustrates a chemical reaction of a coloring material applied to an oral bacteria test rod according to another embodiment of the present invention.
  • ordinal expression expressions such as first and second are intended to describe objects that are equivalent and independent of each other, and in the order of main / sub or master / slave It should be understood as having no meaning.
  • the term "subject” may mean any subject whose oral health is to be monitored.
  • the subject may be any mammal except for humans, but is not limited thereto.
  • the subject within the present specification may be at least one of dogs, cats, rabbits, cows, chickens, pigs, horses, goats, ducks, geese, but is not limited thereto.
  • oral disease refers to diseases occurring in the oral cavity, including periodontitis, gingivitis and periodontal abscess periodontal disease, stomatitis, vitiligo, erythema, papilloma, retention cyst, sublingual sac, palate and mandibular prominence. , hand foot and mouth disease, oral tuberculosis, oral syphilis, oral candidiasis, Sj ⁇ gren's syndrome, and lupus.
  • oral disease in the present specification may be periodontal disease, but is not limited thereto.
  • bacteria associated with oral diseases may be anaerobic bacteria.
  • the bacteria associated with oral disease may be anaerobic bacteria that cause periodontal disease.
  • bacteria associated with oral diseases include Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, and Tanerella porci. It may be at least one periodontal disease-causing bacterium of Tannerella forsythia.
  • bacteria associated with oral diseases may be bacteria causing dental caries, such as Streptococcus mutans or Lactobacillus.
  • bacteria associated with oral diseases can produce specific enzymes such as trypsin like enzymes during metabolism.
  • FIGS. 1 to 3 a companion animal oral health management system based on the oral monitoring system according to an embodiment of the present invention will be described with reference to FIGS. 1 to 3.
  • FIG. 1 and 2 illustratively illustrates the configuration of an oral bacteria test rod according to another embodiment of the present invention.
  • FIG. 3 illustratively illustrates a chemical reaction of a coloring material applied to an oral bacteria test rod according to another embodiment of the present invention.
  • the oral bacteria test rod 300 is placed on the test unit 310 that comes into contact with the teeth or saliva of a companion animal and is placed on the test unit 310 to generate bacteria associated with oral diseases.
  • a color developing unit 320 that reacts with a specific enzyme may be included.
  • the color development unit 320 is configured to change color to yellow when it reacts with a specific enzyme.
  • the oral bacteria test rod 300 in FIG. 1 (a) is manufactured in the form of a strip pad, the present invention is not limited thereto.
  • the oral bacteria test rod 300 includes a cotton swab shape as shown in FIG. 1(b), and may include all shapes that can contact saliva inside the oral cavity.
  • the coloring unit 320 reacts with a specific enzyme produced by bacteria associated with oral diseases, such as a trypsin-like enzyme, at room temperature to produce 4-nitroaniline.
  • a specific enzyme produced by bacteria associated with oral diseases such as a trypsin-like enzyme
  • yellow 4-nitroaniline may be produced in the coloring part 320 according to the reaction between the trypsin enzyme and the coloring material. Accordingly, when bacteria associated with oral diseases that produce trypsin enzyme, particularly anaerobic bacteria, are present, the color developing portion 320 of the oral bacteria test rod 300 may develop a yellow color. At this time, the user can identify the presence of bacteria according to the change in color without separate temperature control equipment, and can recognize the degree of bacteria according to the degree of color. In FIG. 2, the negative control did not develop color, and the lower strip pad was well-colored in yellow, but it was not well distinguished on the black and white drawing, so it was marked with a checkered pattern to indicate that the color was developed.
  • the coloring material on the coloring part 320 reacts with trypsin to produce 4-nitroaniline as a by-product.
  • Tissue Plasminogen Activator (tPA) Substrate or Na-benzoyl-L-arginine 4-nitroanilide hydrochloride (N ⁇ -Benzoyl-L-arginine 4-nitroanilide hydrochloride; BAPNA), but is not limited thereto.
  • the color developing part 320 may be obtained by absorbing a coloring material into a hygroscopic factor such as cellulose paper. Then, the color development unit 320 may be attached on the inspection unit 310 . At this time, the inspection unit 310 may be a polymer material such as plastic, but is not limited thereto.

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Abstract

An oral monitoring system according to one embodiment of the present invention comprises: a communication unit configured to receive a pad image, relating to an oral bacteria test stick which has reacted to teeth or saliva of an object, from a user system; and a processor connected to communicate with the communication unit, wherein the processor may be configured to determine a color change on the basis of the pad image and determine the presence of bacteria associated with an oral disease on the basis of the color change.

Description

구강 박테리아 검사 막대oral bacteria test rod
본 발명은, 구강 박테리아 검사 막대에 관한 것으로, 보다 상세하게는 구강 질환을 야기하는 혐기성 박테리아가 생성하는 특이 효소를 검출하도록 구성된 구강 박테리아 검사 막대에 관한 것이다.The present invention relates to an oral bacteria test rod, and more particularly, to an oral bacteria test rod configured to detect specific enzymes produced by anaerobic bacteria that cause oral diseases.
반려 동물은 구강 관리가 사람보다 어려울 수 있어, 구강 질환을 일으키는 박테리아에 쉽게 노출될 수 있다. 특히, 치구가 쌓이고 치석이 끼게된 경우, 치구 안에 있는 혐기성 박테리아가 치주 질환을 일으킬 수 있다. Pets can have more difficult oral care than humans, making them more susceptible to bacteria that cause oral disease. In particular, when dental plaque is accumulated and tartar is trapped, anaerobic bacteria in dental plaque can cause periodontal disease.
치주 질환의 초기에는 잇몸에 염증이 발생할 수 있는데, 이때, 염증이 치료되지 않을 경우 치주염으로 진행될 수 있다. 결국, 치아가 흔들리게 되어 빠지거나 고름이 고여 원래의 상태로 돌아갈 수 없게 된다.In the early stages of periodontal disease, inflammation may occur in the gums. At this time, if the inflammation is not treated, it may progress to periodontitis. Eventually, the tooth becomes loose and falls out, or pus collects and cannot return to its original state.
한편, 반려견의 치주 질환의 진단을 위해, 엑스레이 촬영과 치주 탐촉자를 이용한 치주 탐침 검사가 있을 수 있다. 그러나 엑스레이 촬영과 치주 탐침 검사는 바이오필름 형성 정도와 치석과 염증 상태를 토대로 검사가 이루어지기 때문에, 예방 목적이 아닌 질병 발생 후 경과 확인용으로 사용되고 있다. On the other hand, for the diagnosis of periodontal disease in companion dogs, there may be X-ray imaging and a periodontal probe test using a periodontal probe. However, since X-ray imaging and periodontal probe inspection are performed based on the degree of biofilm formation, calculus, and inflammation, they are used not for prevention purposes but for checking the progress after the occurrence of disease.
더욱이, 이러한 진단 검사들은 수의 병원을 내원해야만 진행될 수 있다는 한계가 있다.Moreover, these diagnostic tests have limitations in that they can only be performed by visiting a veterinary hospital.
즉, 치주 질환과 같은 구강 질환의 예방하기 위해 장소의 제한 없이 반려 동물의 구강 질환 검출 방법의 지속적으로 요구되고 있는 실정이다. That is, in order to prevent oral diseases such as periodontal disease, there is a continuous demand for a method for detecting oral diseases of companion animals without limitation of location.
발명의 배경이 되는 기술은 본 발명에 대한 이해를 보다 용이하게 하기 위해 작성되었다. 발명의 배경이 되는 기술에 기재된 사항들이 선행기술로 존재한다고 인정하는 것으로 이해되어서는 안 된다.The background description of the invention has been prepared to facilitate understanding of the present invention. It should not be construed as an admission that matters described in the background art of the invention exist as prior art.
(관련기술문헌) 미국특허 제5223403호 공보(Related Art Document) U.S. Patent No. 5223403
전술한 종래의 반려 동물 구강 질환 진단 방법이 갖는 한계를 극복하기 위한 방안으로, 구강 질환 유발균에 특이적으로 발색하는 벤조일-DL-아르기닌-a-나프틸아마이드(benzoyl-DL-arginine-a-naphthylamide)의 발색 물질 기반의 진단 제품이 등장하였다.As a way to overcome the limitations of the above-mentioned conventional companion animal oral disease diagnosis method, benzoyl-DL-arginine-a-naphthylamide (benzoyl-DL-arginine-a- naphthylamide) based diagnostic products appeared.
보다 구체적으로, 종래의 발색 물질 기반의 진단 제품은 면봉, 스트립 패드, 온도 조절 장비로 구성되어 있어, 반려 동물의 구강 타액을 면봉에 적신 후, 면봉을 스트립 패드에 묻혀 온도 조절 장비에 삽입하면 55 ℃에서 발색이 일어날 수 있다. 그 다음, 발색에 기초하여 구강 질환 유발균의 여부가 확인되도록 구성될 수 있다.More specifically, a conventional diagnostic product based on a chromophoric material consists of a cotton swab, a strip pad, and temperature control equipment. After soaking a cotton swab with the oral saliva of a companion animal, the cotton swab is dipped in the strip pad and inserted into the temperature control equipment. Color development may occur at °C. Then, based on the color development, it may be configured to confirm whether oral disease-causing bacteria are present.
즉, 종래의 스트립 패드 기반의 진단 제품은 발색을 위해 특정 온도를 제공하는 온도 저절 장비가 필수일 수 있으며, 반응 결과를 확인하는 것에 있어 현저히 긴 시간이 요구된다는 한계가 존재할 수 있다.That is, a conventional diagnostic product based on a strip pad may require temperature-saving equipment that provides a specific temperature for color development, and there may be a limitation that a remarkably long time is required to confirm the reaction result.
한편, 본 발명의 발명자들은, 종래의 반려 동물 구강 질환 진단 방법이 갖는 한계를 극복하기 위하여 치주 질환의 원인 세균인 혐기성 세균이 특이적으로 생성하는 효소를 검출할 수 있을 뿐만 아니라 상온에서도 색 발현을 일으키는 물질에 주목하였다.On the other hand, the inventors of the present invention, in order to overcome the limitations of the conventional companion animal oral disease diagnosis method, can not only detect enzymes specifically produced by anaerobic bacteria, which are the causative bacteria of periodontal disease, but also color expression at room temperature Attention was paid to the causative agent.
그 결과, 본 발명의 발명자들은 온도 조절 장치 없이도 종래의 발색 물질보다 반응성이 좋아 반응 시간의 단축이 가능하며, 독성이 없는 물질을 발견하기에 이르렀고, 이를 스트립 패드 또는 면봉 형태로 제작하였다. As a result, the inventors of the present invention have found a non-toxic material that is more reactive than conventional chromophoric materials without a temperature control device, enabling shortening of the reaction time, and produced it in the form of a strip pad or cotton swab.
이때, 본 발명의 발명자들은, 새로운 구강 박테리아 검사 막대가 상온에서도 색 발현이 가능하기 때문에 별도의 온도 조절 장비가 필요하지 않아 사용자의 경제적 부담을 줄이고, 사용 편의성을 높일 수 있음을 기대할 수 있었다.At this time, the inventors of the present invention could expect that since the new oral bacteria test rod can express color even at room temperature, additional temperature control equipment is not required, thereby reducing the user's economic burden and increasing convenience of use.
즉, 본 발명은 상술한 기술적 문제에 대응하기 위하여 안출된 것으로, 본 발명의 목적은 종래 기술에서의 한계와 단점에 의해 발생하는 다양한 문제점을 실질적으로 보완할 수 있는 것이다. 특히 본 발명의 목적은, 반려 동물에 대한 구강 건강을 지속적으로 모니터링할 수 있는, 구강 박테리아 검사 막대를 제공하는데 있다.That is, the present invention has been devised to cope with the above-described technical problems, and an object of the present invention is to substantially supplement various problems caused by limitations and disadvantages in the prior art. In particular, an object of the present invention is to provide an oral bacteria test rod capable of continuously monitoring the oral health of a companion animal.
과 연관된 세균이 생성하는 특이 효소와 반응하는 발색 물질을 포함한다. 이때, 발색 물질은 특이 효소와 반응하여 4-나이트로아닐린 (4-nitroaniline) 을 생성한다.Contains a chromogenic substance that reacts with a specific enzyme produced by bacteria associated with At this time, the coloring material reacts with a specific enzyme to produce 4-nitroaniline.
본 발명의 특징에 따르면, 발색 물질은, 상온에서 특이 효소와 반응하여 4-나이트로아닐린 (4-nitroaniline) 을 생성하여 색 발현을 유도할 수 있다. According to the features of the present invention, the chromogenic substance reacts with a specific enzyme at room temperature to produce 4-nitroaniline to induce color expression.
본 발명의 다른 특징에 따르면 상기 특이 효소는 아그레가티박터 액티노마이세템코미탄스 (Aggregatibacter actinomycetemcomitans), 포르피로모나스 진지발리스 (Porphyromonas gingivalis), 프레보텔라 인터메디아 (Prevotella intermedia), 타네렐라 포르시시아 (Tannerella forsythia), 스트렙토코커스 뮤탄스(Streptococcus mutans) 및 락토바실러스 (Lactobacillus) 중 적어도 하나에서 발생하는 트립신 유사 효소(trypsin like enzyme)를 포함할 수 있다. According to another feature of the present invention, the specific enzyme is Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tanerella spor It may include a trypsin like enzyme generated from at least one of Tannerella forsythia, Streptococcus mutans and Lactobacillus.
본 발명의 다른 특징에 따르면 발색 물질은, 조직 플라스미노겐 활성화 물질 (Tissue Plasminogen Activator; tPA) 기질 (substrate) 또는, 나-벤조일-L-아르기닌 4-나이트로아닐라이드 하이드로클로라이드 (Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride; BAPNA) 일 수 있다.According to another feature of the present invention, the chromogenic material is a tissue plasminogen activator (tPA) substrate or Na-benzoyl-L-arginine 4-nitroanilide hydrochloride (Nα-Benzoyl- L-arginine 4-nitroanilide hydrochloride; BAPNA).
기타 실시예의 구체적인 사항들은 상세한 설명 및 도면들에 포함되어 있다.Other embodiment specifics are included in the detailed description and drawings.
본 발명은, 구강 질환 연관 세균과 특이적으로 반응하여 발색 물질을 구강 모니터링 시스템을 제공함에 따라, 종래의 엑스레이 촬영 및/또는 탐침 검사 기반의 구강 질환 진단 방법, 나아가 온도 조절 장비의 구비가 요구되는 종래 스트립 패드 기반의 진단 제품의 한계를 극복할 수 있는 효과가 있다. As the present invention provides an oral monitoring system that reacts specifically with oral disease-related bacteria to produce a chromogenic substance, a conventional oral disease diagnosis method based on X-ray imaging and / or probing, and furthermore, temperature control equipment is required. There is an effect that can overcome the limitations of conventional strip pad-based diagnostic products.
보다 구체적으로, 본 발명은 상온에서 발색하며, 종래의 발색 물질보다 반응성이 좋아 반응 시간의 단축이 가능하며, 독성이 없는 발색 물질 기반의 구강 모니터링 시스템을 제공할 수 있다.More specifically, the present invention can provide an oral monitoring system based on a chromophoric substance that develops at room temperature, is more reactive than conventional chromophoric substances, can shorten reaction time, and is non-toxic.
즉, 본 발명은, 상온에서도 색 발현이 가능하기 때문에 별도의 온도 조절 장비가 필요하지 않아 사용자의 경제적 부담을 줄이고, 사용 편의성을 높일 수 있다.That is, the present invention can reduce the user's economic burden and increase the convenience of use because a separate temperature control device is not required because color expression is possible even at room temperature.
나아가, 본 발명은, 보다 간단하게 구강 질환 유발 세균의 유무를 확인하여 치주 질환과 같은 구강 질환을 예방할 수 있는 효과가 있다. Furthermore, the present invention has an effect of preventing oral diseases such as periodontal disease by more simply checking the presence or absence of oral disease-causing bacteria.
나아가 본 발명은, 구강 질환의 조기 진단이 가능하여 중증 단계로 넘어가기 전에 반려 동물에 적절한 치료가 수행될 수 있어, 좋은 치료 예후에 기여할 수 있다.Furthermore, the present invention can contribute to a good treatment prognosis because it is possible to diagnose oral diseases early, so that appropriate treatment can be performed on companion animals before they go to a severe stage.
본 발명에 따른 효과는 이상에서 예시된 내용에 의해 제한되지 않으며, 더욱 다양한 효과들이 본 명세서 내에 포함되어 있다.Effects according to the present invention are not limited by the contents exemplified above, and more various effects are included in the present specification.
도 1 및 도 2는 본 발명의 다른 실시예에 따른 구강 박테리아 검사 막대의 구성을 예시적으로 도시한 것이다. 1 and 2 illustratively illustrates the configuration of an oral bacteria test rod according to another embodiment of the present invention.
도 3은 본 발명의 다른 실시예에 따른 구강 박테리아 검사 막대에 적용되는 발색 물질의 화학 반응을 예시적으로 도시한 것이다. FIG. 3 illustratively illustrates a chemical reaction of a coloring material applied to an oral bacteria test rod according to another embodiment of the present invention.
이하의 내용은 단지 발명의 원리를 예시한다. 그러므로 당업자는 비록 본 명세서에 명확히 설명되거나 도시되지 않았지만 발명의 원리를 구현하고 발명의 개념과 범위에 포함된 다양한 장치를 발명할 수 있는 것이다. 또한, 본 명세서에 열거된 모든 조건부 용어 및 실시예들은 원칙적으로, 발명의 개념이 이해되도록 하기 위한 목적으로만 명백히 의도되고, 이와 같이 특별히 열거된 실시예들 및 상태들에 제한적이지 않는 것으로 이해되어야 한다. The following merely illustrates the principle of the invention. Therefore, those skilled in the art can invent various devices that embody the principles of the invention and fall within the concept and scope of the invention, even though not explicitly described or shown herein. In addition, it should be understood that all conditional terms and embodiments listed in this specification are, in principle, expressly intended only for the purpose of making the concept of the invention understood, and not limited to such specifically listed embodiments and conditions. do.
또한, 이하의 설명에서 제1, 제2 등과 같은 서수식 표현은 서로 동등하고 독립된 객체를 설명하기 위한 것이며, 그 순서에 주(main)/부(sub) 또는 주(master)/종(slave)의 의미는 없는 것으로 이해되어야 한다.In addition, in the following description, ordinal expression expressions such as first and second are intended to describe objects that are equivalent and independent of each other, and in the order of main / sub or master / slave It should be understood as having no meaning.
상술한 목적, 특징 및 장점은 첨부된 도면과 관련한 다음의 상세한 설명을 통하여 보다 분명해질 것이며, 그에 따라 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 발명의 기술적 사상을 용이하게 실시할 수 있을 것이다. The above objects, features and advantages will become more apparent through the following detailed description in conjunction with the accompanying drawings, and accordingly, those skilled in the art to which the invention belongs will be able to easily implement the technical idea of the invention. .
본 발명의 여러 실시예들의 각각 특징들이 부분적으로 또는 전체적으로 서로 결합 또는 조합 가능하며, 당업자가 충분히 이해할 수 있듯이 기술적으로 다양한 연동 및 구동이 가능하며, 각 실시예들이 서로에 대하여 독립적으로 실시 가능할 수도 있고 연관 관계로 함께 실시 가능할 수도 있다.Each feature of the various embodiments of the present invention can be partially or entirely combined or combined with each other, and as those skilled in the art can fully understand, various interlocking and driving operations are possible, and each embodiment can be implemented independently of each other. It may be possible to implement together in an association relationship.
본 명세서의 해석의 명확함을 위해, 이하에서는 본 명세서에서 사용되는 용어들을 정의하기로 한다.For clarity of interpretation of this specification, terms used in this specification will be defined below.
본 명세서에서 사용되는 용어, "개체"는 구강 건강을 모니터링하고자 하는 모든 대상을 의미할 수 있다. 이때, 개체는, 인간을 제외한 모든 포유 동물일 수 있으나, 이에 제한되는 것은 아니다. 바람직하게, 본원 명세서 내에서 개체는, 개, 고양이, 토끼, 소, 닭, 돼지, 말, 염소, 오리, 거위, 중 적어도 하나일 수 있으나, 이에 제한되는 것은 아니다.As used herein, the term "subject" may mean any subject whose oral health is to be monitored. In this case, the subject may be any mammal except for humans, but is not limited thereto. Preferably, the subject within the present specification may be at least one of dogs, cats, rabbits, cows, chickens, pigs, horses, goats, ducks, geese, but is not limited thereto.
본 명세서에서 사용되는 용어, "구강 질환"은 구내에서 일어나는 질환을 의미하는 것으로, 치주염, 치은염 및 치주 농양의 치주 질환, 구내염, 백반증, 적색반, 유두종, 저류낭, 설하낭, 구개 및 하악융기, 수족구병, 구강결핵, 구강매독, 구강 칸디다증, 쇼그렌 (Sjφgren) 증후군, 루프스 중 적어도 하나일 수 있다.As used herein, the term "oral disease" refers to diseases occurring in the oral cavity, including periodontitis, gingivitis and periodontal abscess periodontal disease, stomatitis, vitiligo, erythema, papilloma, retention cyst, sublingual sac, palate and mandibular prominence. , hand foot and mouth disease, oral tuberculosis, oral syphilis, oral candidiasis, Sjφgren's syndrome, and lupus.
바람직하게, 본원 명세서에서 구강 질환은 치주 질환일 수 있으나, 이에 제한되는 것은 아니다.Preferably, oral disease in the present specification may be periodontal disease, but is not limited thereto.
본 명세서에서 사용되는 용어, "구강 질환과 연관된 세균"은 혐기성 세균일 수 있다. 바람직하게, 구강 질환과 연관된 세균은, 치주 질환을 야기하는 혐기성 세균일 수 있다.As used herein, the term "bacteria associated with oral diseases" may be anaerobic bacteria. Preferably, the bacteria associated with oral disease may be anaerobic bacteria that cause periodontal disease.
예를 들어, 구강 질환과 연관된 세균은 아그레가티박터 액티노마이세템코미탄스 (Aggregatibacter actinomycetemcomitans), 포르피로모나스 진지발리스 (Porphyromonas gingivalis), 프레보텔라 인터메디아 (Prevotella intermedia) 및 타네렐라 포르시시아 (Tannerella forsythia) 중 적어도 하나의 치주 질환 유발 세균일 수 있다.For example, bacteria associated with oral diseases include Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, and Tanerella porci. It may be at least one periodontal disease-causing bacterium of Tannerella forsythia.
그러나 이에 제한되는 것은 아니며, 구강 질환과 연관된 세균은 스트렙토코커스 뮤탄스 (Streptococcus mutans) 또는 락토바실러스 (Lactobacillus) 와 같은 치아 우식증 유발 세균일 수도 있다.However, it is not limited thereto, and bacteria associated with oral diseases may be bacteria causing dental caries, such as Streptococcus mutans or Lactobacillus.
한편, 구강 질환과 연관된 세균은, 대사 중에 트립신 유사 효소 (trypsin like enzyme) 와 같은 특이 효소를 생성할 수 있다. On the other hand, bacteria associated with oral diseases can produce specific enzymes such as trypsin like enzymes during metabolism.
이하에서는 도 1 내지 도 3을 참조하여, 본 발명의 일 실시예에 따른 구강 모니터링 시스템에 기초한 반려 동물 구강 건강 관리 시스템을 설명한다. Hereinafter, a companion animal oral health management system based on the oral monitoring system according to an embodiment of the present invention will be described with reference to FIGS. 1 to 3.
도 1 및 도 2는 본 발명의 다른 실시예에 따른 구강 박테리아 검사 막대의 구성을 예시적으로 도시한 것이다. 도 3은 본 발명의 다른 실시예에 따른 구강 박테리아 검사 막대에 적용되는 발색 물질의 화학 반응을 예시적으로 도시한 것이다. 1 and 2 illustratively illustrates the configuration of an oral bacteria test rod according to another embodiment of the present invention. FIG. 3 illustratively illustrates a chemical reaction of a coloring material applied to an oral bacteria test rod according to another embodiment of the present invention.
먼저, 도 1(a)을 참조하면, 구강 박테리아 검사 막대(300)는, 반려 동물의 치아 또는 타액과 접촉하는 검사부(310), 검사부(310)상에 배치되어 구강 질환과 연관된 세균이 생성하는 특이 효소와 반응하는 발색부(320)를 포함할 수 있다. 발색부(320)는 특이 효소와 반응하면 노란색으로 변색되는 구성이다. 한편, 도 1(a)에서 구강 박테리아 검사 막대(300)는 스트립 패드 형태로 제작되었으나, 본 발명은 이에 한정되는 것이 아니다. 구강 박테리아 검사 막대(300)는 도 1(b)와 같이 면봉 형태를 포함하며, 구강 내부의 타액과 접촉할 수 있는 형태를 모두 포함할 수 있다. First, referring to FIG. 1 (a), the oral bacteria test rod 300 is placed on the test unit 310 that comes into contact with the teeth or saliva of a companion animal and is placed on the test unit 310 to generate bacteria associated with oral diseases. A color developing unit 320 that reacts with a specific enzyme may be included. The color development unit 320 is configured to change color to yellow when it reacts with a specific enzyme. On the other hand, although the oral bacteria test rod 300 in FIG. 1 (a) is manufactured in the form of a strip pad, the present invention is not limited thereto. The oral bacteria test rod 300 includes a cotton swab shape as shown in FIG. 1(b), and may include all shapes that can contact saliva inside the oral cavity.
도 2를 함께 참조하면, 발색부(320)는, 상온에서 트립신 유사 효소와 같은 구강 질환과 연관된 세균이 생성하는 특이 효소와 반응하여 4-나이트로아닐린(4-nitroaniline)을 생성하도록 구성된 발색 물질로 이루어질 수 있다.Referring to FIG. 2 together, the coloring unit 320 reacts with a specific enzyme produced by bacteria associated with oral diseases, such as a trypsin-like enzyme, at room temperature to produce 4-nitroaniline. can be made with
이때, 트립신 효소와 발색 물질의 반응에 따라 발색부(320)는, 음성 대조군 (negative control) 과 대조적으로, 노란색의 4-나이트로아닐린이 생성될 수 있다. 이에, 트립신 효소를 생성하는 구강 질환과 연관된 세균, 특히 혐기성 세균이 존재할 경우, 구강 박테리아 검사 막대(300)의 발색부(320)는 노란색을 발색할 수 있다. 이때, 사용자는 별도의 온도 조절 장비 없이도 색상의 변화에 따라 세균의 존재를 식별할 수 있고, 색상의 정도에 따라 세균의 정도를 인지할 수 있다. 도 2에서는음성 대조군(negative control)은 발색되지 않았고, 아랫쪽 스트립 패드는 노란색으로 잘 발색되었으나, 흑백 도면상에서 잘 구분되지 않아 발색되었음을 표시하기 위하여 체크무늬로 표시하였다. At this time, in contrast to the negative control, yellow 4-nitroaniline may be produced in the coloring part 320 according to the reaction between the trypsin enzyme and the coloring material. Accordingly, when bacteria associated with oral diseases that produce trypsin enzyme, particularly anaerobic bacteria, are present, the color developing portion 320 of the oral bacteria test rod 300 may develop a yellow color. At this time, the user can identify the presence of bacteria according to the change in color without separate temperature control equipment, and can recognize the degree of bacteria according to the degree of color. In FIG. 2, the negative control did not develop color, and the lower strip pad was well-colored in yellow, but it was not well distinguished on the black and white drawing, so it was marked with a checkered pattern to indicate that the color was developed.
도 3의 (a) 및 (b)를 함께 참조하면, 발색부(320)상의 발색 물질은 트립신과 반응하여 4-나이트로아닐린을 부산물로 생성하는 조직 플라스미노겐 활성화 물질 (Tissue Plasminogen Activator; tPA) 기질 (substrate) 또는, 나-벤조일-L-아르기닌 4-나이트로아닐라이드 하이드로클로라이드 (Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride; BAPNA) 일 수 있으나, 이에 제한되는 것은 아니다.Referring to (a) and (b) of FIG. 3 together, the coloring material on the coloring part 320 reacts with trypsin to produce 4-nitroaniline as a by-product. Tissue Plasminogen Activator (tPA) ) Substrate or Na-benzoyl-L-arginine 4-nitroanilide hydrochloride (Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride; BAPNA), but is not limited thereto.
한편, 발색부(320)는 발색 물질이 셀룰로오스 종이와 같은 흡습성 인자에 흡수됨으로써 획득될 수 있다. 그 다음, 발색부(320)는 검사부(310) 상에 부착될 수 있다. 이때, 검사부(310)는 플라스틱과 같은 고분자 소재일 수 있으나, 이에 제한되는 것은 아니다. Meanwhile, the color developing part 320 may be obtained by absorbing a coloring material into a hygroscopic factor such as cellulose paper. Then, the color development unit 320 may be attached on the inspection unit 310 . At this time, the inspection unit 310 may be a polymer material such as plastic, but is not limited thereto.
이상 첨부된 도면을 참조하여 본 발명의 실시예들을 더욱 상세하게 설명하였으나, 본 발명은 반드시 이러한 실시예로 국한되는 것은 아니고, 본 발명의 기술사상을 벗어나지 않는 범위 내에서 다양하게 변형 실시될 수 있다. 따라서, 본 발명에 개시된 실시예들은 본 발명의 기술 사상을 한정하기 위한 것이 아니라 설명하기 위한 것이고, 이러한 실시예에 의하여 본 발명의 기술 사상의 범위가 한정되는 것은 아니다. 그러므로, 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해해야만 한다. 본 발명의 보호 범위는 아래의 청구범위에 의하여 해석되어야 하며, 그와 동등한 범위 내에 있는 모든 기술 사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.Although the embodiments of the present invention have been described in more detail with reference to the accompanying drawings, the present invention is not necessarily limited to these embodiments, and may be variously modified and implemented without departing from the technical spirit of the present invention. . Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain, and the scope of the technical idea of the present invention is not limited by these embodiments. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The protection scope of the present invention should be construed according to the claims below, and all technical ideas within the equivalent range should be construed as being included in the scope of the present invention.

Claims (4)

  1. 개체의 치아 또는 타액과 접촉하는 검사부;an inspection unit that contacts teeth or saliva of an object;
    상기 검사부 상의, 구강 질환과 연관된 세균이 생성하는 특이 효소와 반응하는 발색 물질을 포함하고,On the inspection unit, a color-developing material that reacts with a specific enzyme produced by bacteria associated with oral diseases is included,
    상기 발색 물질은 상기 특이 효소와 반응하여 4-나이트로아닐린 (4-nitroaniline)을 생성하는,The chromogenic material reacts with the specific enzyme to produce 4-nitroaniline,
    구강 박테리아 검사 막대.Oral Bacteria Test Rod.
  2. 제1항에 있어서,According to claim 1,
    상기 발색 물질은, 상온에서 상온에서 특이 효소와 반응하여 4-나이트로아닐린 (4-nitroaniline) 을 생성하여 색 발현을 유도하도록 구성된, 구강 박테리아 검사 막대. The oral bacteria test rod, wherein the coloring material is configured to react with a specific enzyme at room temperature to produce 4-nitroaniline to induce color expression.
  3. 제 2 항에 있어서,According to claim 2,
    상기 특이 효소는 아그레가티박터 액티노마이세템코미탄스 (Aggregatibacter actinomycetemcomitans), 포르피로모나스 진지발리스 (Porphyromonas gingivalis), 프레보텔라 인터메디아 (Prevotella intermedia), 타네렐라 포르시시아 (Tannerella forsythia), 스트렙토코커스 뮤탄스(Streptococcus mutans) 및 락토바실러스 (Lactobacillus) 중 적어도 하나에서 발생하는 트립신 유사 효소(trypsin like enzyme)를 포함하는, 구강 박테리아 검사 막대.The specific enzyme is Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, An oral bacteria test rod comprising a trypsin like enzyme occurring in at least one of Streptococcus mutans and Lactobacillus.
  4. 제3항에 있어서,According to claim 3,
    상기 발색 물질은, The coloring material is
    조직 플라스미노겐 활성화 물질 (Tissue Plasminogen Activator; tPA) 기질 (substrate) 또는, Tissue Plasminogen Activator (tPA) substrate or
    나-벤조일-L-아르기닌 4-나이트로아닐라이드 하이드로클로라이드 (Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride; BAPNA) 인, 구강 박테리아 검사 막대.Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride (BAPNA), oral bacteria test bar.
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