WO2022197752A1 - Sélection d'ensembles de poids basée sur l'emplacement d'un élément et/ou le cycle pour appel de base - Google Patents

Sélection d'ensembles de poids basée sur l'emplacement d'un élément et/ou le cycle pour appel de base Download PDF

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WO2022197752A1
WO2022197752A1 PCT/US2022/020460 US2022020460W WO2022197752A1 WO 2022197752 A1 WO2022197752 A1 WO 2022197752A1 US 2022020460 W US2022020460 W US 2022020460W WO 2022197752 A1 WO2022197752 A1 WO 2022197752A1
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Prior art keywords
weights
sensing cycles
weight set
subseries
data
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PCT/US2022/020460
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English (en)
Inventor
Gavin Derek PARNABY
Mark David Hahm
Andrew Christopher Du Preez
Dorna KASHEFHAGHIGHI
Kishore Jaganathan
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Illumina, Inc.
Illumina Software, Inc.
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Publication date
Priority claimed from US17/687,551 external-priority patent/US20220301657A1/en
Application filed by Illumina, Inc., Illumina Software, Inc. filed Critical Illumina, Inc.
Priority to KR1020227045560A priority Critical patent/KR20230157230A/ko
Priority to AU2022238841A priority patent/AU2022238841A1/en
Priority to CN202280005111.4A priority patent/CN115803815A/zh
Priority to EP22714689.1A priority patent/EP4309179A1/fr
Priority to IL299077A priority patent/IL299077A/en
Priority to CA3183581A priority patent/CA3183581A1/fr
Priority to JP2022580969A priority patent/JP2024510539A/ja
Publication of WO2022197752A1 publication Critical patent/WO2022197752A1/fr

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    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06NCOMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
    • G06N3/00Computing arrangements based on biological models
    • G06N3/02Neural networks
    • G06N3/04Architecture, e.g. interconnection topology
    • G06N3/045Combinations of networks
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06NCOMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
    • G06N3/00Computing arrangements based on biological models
    • G06N3/02Neural networks
    • G06N3/06Physical realisation, i.e. hardware implementation of neural networks, neurons or parts of neurons
    • G06N3/063Physical realisation, i.e. hardware implementation of neural networks, neurons or parts of neurons using electronic means
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20Supervised data analysis
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06NCOMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
    • G06N3/00Computing arrangements based on biological models
    • G06N3/02Neural networks
    • G06N3/04Architecture, e.g. interconnection topology
    • G06N3/048Activation functions

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  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Biophysics (AREA)
  • Data Mining & Analysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Software Systems (AREA)
  • Biomedical Technology (AREA)
  • Evolutionary Computation (AREA)
  • Medical Informatics (AREA)
  • Artificial Intelligence (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mathematical Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Computing Systems (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Computational Linguistics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Public Health (AREA)
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  • Databases & Information Systems (AREA)
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  • Neurology (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compression, Expansion, Code Conversion, And Decoders (AREA)

Abstract

Système d'appel de bases comprenant une mémoire stockant une topologie d'un réseau neuronal, une pluralité d'ensembles de poids, et des données de capteur pour une série de cycles de détection. Des événements de séquençage s'étendent sur une courbe temporelle de l'opération d'appel de base tout au long d'une sous-série de cycles de détection, et sur une courbe spatiale de l'opération d'appel de base à travers des emplacements sur un biocapteur. Un processeur configurable est configuré pour charger la topologie sur le processeur configurable, sélectionner un ensemble de poids en fonction d'une sous-série donnée de cycles de détection et/ou d'un emplacement donné sur le biocapteur, charger des données de capteur donné pour la sous-série donnée de cycles de détection et l'emplacement donné sur les éléments de traitement, configurer la topologie à l'aide de l'ensemble de poids sélectionné, et amener le réseau neuronal à traiter les données de capteur donné pour produire des données de classification d'appel de base pour la sous-série donnée et l'emplacement donné.
PCT/US2022/020460 2021-03-16 2022-03-15 Sélection d'ensembles de poids basée sur l'emplacement d'un élément et/ou le cycle pour appel de base WO2022197752A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1020227045560A KR20230157230A (ko) 2021-03-16 2022-03-15 염기 호출을 위한 타일 위치 및/또는 사이클 기반 가중치 세트 선택
AU2022238841A AU2022238841A1 (en) 2021-03-16 2022-03-15 Tile location and/or cycle based weight set selection for base calling
CN202280005111.4A CN115803815A (zh) 2021-03-16 2022-03-15 用于碱基检出的基于区块位置和/或循环的权重集选择
EP22714689.1A EP4309179A1 (fr) 2021-03-16 2022-03-15 Sélection d'ensembles de poids basée sur l'emplacement d'un élément et/ou le cycle pour appel de base
IL299077A IL299077A (en) 2021-03-16 2022-03-15 Placing tiles and/or choosing a cycle-based weight set for naming the bases
CA3183581A CA3183581A1 (fr) 2021-03-16 2022-03-15 Selection d'ensembles de poids basee sur l'emplacement d'un element et/ou le cycle pour appel de base
JP2022580969A JP2024510539A (ja) 2021-03-16 2022-03-15 ベースコールのためのタイル位置及び/又はサイクルベースの重みセット選択

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US202163161896P 2021-03-16 2021-03-16
US202163161880P 2021-03-16 2021-03-16
US63/161,896 2021-03-16
US63/161,880 2021-03-16
US17/687,583 2022-03-04
US17/687,551 2022-03-04
US17/687,551 US20220301657A1 (en) 2021-03-16 2022-03-04 Tile location and/or cycle based weight set selection for base calling
US17/687,583 US20220300811A1 (en) 2021-03-16 2022-03-04 Neural network parameter quantization for base calling

Publications (1)

Publication Number Publication Date
WO2022197752A1 true WO2022197752A1 (fr) 2022-09-22

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PCT/US2022/020460 WO2022197752A1 (fr) 2021-03-16 2022-03-15 Sélection d'ensembles de poids basée sur l'emplacement d'un élément et/ou le cycle pour appel de base
PCT/US2022/020462 WO2022197754A1 (fr) 2021-03-16 2022-03-15 Quantification de paramètres de réseau neuronal pour appel de base

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2022/020462 WO2022197754A1 (fr) 2021-03-16 2022-03-15 Quantification de paramètres de réseau neuronal pour appel de base

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Citations (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (fr) 1989-10-26 1991-05-16 Sri International Sequençage d'adn
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
US20040096853A1 (en) 2000-12-08 2004-05-20 Pascal Mayer Isothermal amplification of nucleic acids on a solid support
WO2005065814A1 (fr) 2004-01-07 2005-07-21 Solexa Limited Arrangements moleculaires modifies
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2006064199A1 (fr) 2004-12-13 2006-06-22 Solexa Limited Procede ameliore de detection de nucleotides
US7115400B1 (en) 1998-09-30 2006-10-03 Solexa Ltd. Methods of nucleic acid amplification and sequencing
US20060240439A1 (en) 2003-09-11 2006-10-26 Smith Geoffrey P Modified polymerases for improved incorporation of nucleotide analogues
US20060281471A1 (en) 2005-06-08 2006-12-14 Cisco Technology,Inc. Method and system for communicating using position information
WO2007010251A2 (fr) 2005-07-20 2007-01-25 Solexa Limited Preparation de matrices pour sequencage d'acides nucleiques
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US20070128624A1 (en) 2005-11-01 2007-06-07 Gormley Niall A Method of preparing libraries of template polynucleotides
WO2007123744A2 (fr) 2006-03-31 2007-11-01 Solexa, Inc. Systèmes et procédés pour analyse de séquençage par synthèse
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
US7329492B2 (en) 2000-07-07 2008-02-12 Visigen Biotechnologies, Inc. Methods for real-time single molecule sequence determination
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US20080234136A1 (en) 2005-06-15 2008-09-25 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7592435B2 (en) 2005-08-19 2009-09-22 Illumina Cambridge Limited Modified nucleosides and nucleotides and uses thereof
US7595882B1 (en) 2008-04-14 2009-09-29 Geneal Electric Company Hollow-core waveguide-based raman systems and methods
US20130079232A1 (en) 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10152776B2 (en) * 2017-03-07 2018-12-11 Illumina, Inc. Optical distortion correction for imaged samples
US20200302224A1 (en) * 2019-03-21 2020-09-24 Illumina, Inc. Artificial Intelligence-Based Sequencing
CA3104851A1 (fr) * 2019-05-16 2020-11-19 Illumina, Inc. Appel de base au moyen de convolutions

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10491239B1 (en) * 2017-02-02 2019-11-26 Habana Labs Ltd. Large-scale computations using an adaptive numerical format
KR20200043169A (ko) * 2018-10-17 2020-04-27 삼성전자주식회사 뉴럴 네트워크의 파라미터를 양자화하는 방법 및 장치

Patent Citations (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (fr) 1989-10-26 1991-05-16 Sri International Sequençage d'adn
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
US7115400B1 (en) 1998-09-30 2006-10-03 Solexa Ltd. Methods of nucleic acid amplification and sequencing
US7329492B2 (en) 2000-07-07 2008-02-12 Visigen Biotechnologies, Inc. Methods for real-time single molecule sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US20040096853A1 (en) 2000-12-08 2004-05-20 Pascal Mayer Isothermal amplification of nucleic acids on a solid support
US20060188901A1 (en) 2001-12-04 2006-08-24 Solexa Limited Labelled nucleotides
US7566537B2 (en) 2001-12-04 2009-07-28 Illumina Cambridge Limited Labelled nucleotides
US7427673B2 (en) 2001-12-04 2008-09-23 Illumina Cambridge Limited Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
US7541444B2 (en) 2002-08-23 2009-06-02 Illumina Cambridge Limited Modified nucleotides
US20070166705A1 (en) 2002-08-23 2007-07-19 John Milton Modified nucleotides
US20060240439A1 (en) 2003-09-11 2006-10-26 Smith Geoffrey P Modified polymerases for improved incorporation of nucleotide analogues
WO2005065814A1 (fr) 2004-01-07 2005-07-21 Solexa Limited Arrangements moleculaires modifies
US20110059865A1 (en) 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
WO2006064199A1 (fr) 2004-12-13 2006-06-22 Solexa Limited Procede ameliore de detection de nucleotides
US20060281471A1 (en) 2005-06-08 2006-12-14 Cisco Technology,Inc. Method and system for communicating using position information
US20080234136A1 (en) 2005-06-15 2008-09-25 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
WO2007010251A2 (fr) 2005-07-20 2007-01-25 Solexa Limited Preparation de matrices pour sequencage d'acides nucleiques
US7592435B2 (en) 2005-08-19 2009-09-22 Illumina Cambridge Limited Modified nucleosides and nucleotides and uses thereof
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US20070128624A1 (en) 2005-11-01 2007-06-07 Gormley Niall A Method of preparing libraries of template polynucleotides
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
WO2007123744A2 (fr) 2006-03-31 2007-11-01 Solexa, Inc. Systèmes et procédés pour analyse de séquençage par synthèse
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7595882B1 (en) 2008-04-14 2009-09-29 Geneal Electric Company Hollow-core waveguide-based raman systems and methods
US20130079232A1 (en) 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10152776B2 (en) * 2017-03-07 2018-12-11 Illumina, Inc. Optical distortion correction for imaged samples
US20200302224A1 (en) * 2019-03-21 2020-09-24 Illumina, Inc. Artificial Intelligence-Based Sequencing
CA3104851A1 (fr) * 2019-05-16 2020-11-19 Illumina, Inc. Appel de base au moyen de convolutions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BENTLEY ET AL., NATURE, vol. 456, 2008, pages 53 - 59

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