WO2022197376A1 - Fullerene phosphonate poly di-galloyls and methods - Google Patents

Fullerene phosphonate poly di-galloyls and methods Download PDF

Info

Publication number
WO2022197376A1
WO2022197376A1 PCT/US2022/014454 US2022014454W WO2022197376A1 WO 2022197376 A1 WO2022197376 A1 WO 2022197376A1 US 2022014454 W US2022014454 W US 2022014454W WO 2022197376 A1 WO2022197376 A1 WO 2022197376A1
Authority
WO
WIPO (PCT)
Prior art keywords
egcg
poly
molecule
composition
bonded
Prior art date
Application number
PCT/US2022/014454
Other languages
French (fr)
Inventor
Peter Butzloff
Original Assignee
Sinapu Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2022/012369 external-priority patent/WO2022159332A1/en
Application filed by Sinapu Llc filed Critical Sinapu Llc
Priority to EP22771889.7A priority Critical patent/EP4308532A1/en
Priority to CN202280032624.4A priority patent/CN117295704A/en
Priority to US17/592,899 priority patent/US20220288212A1/en
Publication of WO2022197376A1 publication Critical patent/WO2022197376A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
    • C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
    • C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
    • A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A61K9/006—Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/007—Pulmonary tract; Aromatherapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
    • C07H13/08—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals directly attached to carbocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner

Definitions

  • the present invention is a composition of buckminsterfullerene with one or more polygalloyl functional groups, where these can be a quinic acid ester (catechin) or a benzo- hydropyran of at least one of a tri-hydroxyphenyl group or a di-hydroxyphenyl group and may also include disodium phosphonate groups to maintain or re-establish hormesis and healthy cellular homeostasis.
  • a quinic acid ester catechin
  • a benzo- hydropyran of at least one of a tri-hydroxyphenyl group or a di-hydroxyphenyl group and may also include disodium phosphonate groups to maintain or re-establish hormesis and healthy cellular homeostasis.
  • One formulation of this composition is in topical beauty products to mitigate the cellular effects of skin aging.
  • Another formulation of this composition combats bacterial infections such as periodontal disease, especially in the case of antibiotic resistant bacteria.
  • the utility of this and other uses simultaneously and synergistieally is to prevent or help treat uncontrolled cellular proliferation, neoplasms, degenerative malignancy associated with cancers, and some chronic inflammatory diseases such as Alzheimer’s Disease, Parkinson’s Disease, and respiratory ailments such as chronic obstructive pulmonary disease (COPD), in susceptible cells.
  • the provided delivery methods include ingestion, topical application, buccal application, inhalation, or injection.
  • the composition can be formulated as a prophylactic medicament or used as a food supplement.
  • Green tea is recognized as the most effective cancer prevention drink in the world.
  • the cancer protection properties of various tea extracts are widely known and are clearly linked to a group of polyphenols of a type of flavanol derivatives, or flavonoids.
  • These fiavonoids have a chemical structure known as a flavan-3-ol that each possess a 2 -phenyl-3, 4-dihydro-2H- chromen-3-ol skeleton.
  • these fiavan-3-ols there are four highly similar, structurally related catechin molecules that have been identified in green tea as being most able to prevent the development of cancer.
  • catechins are EC (epicatechin), epicatechin gallate (ECG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG).
  • a different class of molecules also having benzo hydropyran groups are the tannins, which have at least one central glucose molecule as their distinguishing characteristic feature. Once benzene is derivatized with hydroxyl groups (OH) these groups are also known as phenyl groups.
  • OH hydroxyl groups
  • tannins There are many types of tannins, and their structures may vary widely in the number of di-phenyl benzene and tri-phenyl benzene groups. Many of the tannins can become too large to be of effective use because of poor solubility, especially when multiple glucose groups become entrained into their structures.
  • tannic acid a very simple one, called penta-m-digalloyl-glucose
  • penta-m-digalloyl-glucose a very simple one, called penta-m-digalloyl-glucose
  • tannic acid is reproducibly about equal in anti-proliferative function to the efficacy of EGCG, which points to both EGCG and tannic acid as ideal candidates for future development to enhance their anti-cancer properties. It is thought that the anti-cancer properties in both EGCG and tannic acid arises because they express di-hydroxy phenyl or tri-hydroxy phenyl (more generally, polygalloyl) functionality in their outer molecular structures.
  • EGCG has a maximum solubility at room temperature of 92 grams per liter of water.
  • EGCG from green tea can induce apoptosis in cancerous cells. There is at most about 1 .25 grams per liter of EGCG in brewed green tea. Epidemiological studies indicate that a minimum of from 2.5 to 3 grams of EGCG ingested per day yields a blood serum concentration of 0.1-1 mM EGCG to obtain some decreased risk of cancers.
  • EGCG polyphenol confers beneficial effects against several (chronic) pathologies associated with oxidative stress damage to cells, Including multiple types of cancer, cardiovascular disease, and neurodegenerative diseases, in addition, EGCG provides significant antibacterial and antiviral properties that find use in Alzheimer’s disease.
  • Anti-cancer properties of EGCG arising from activity on p53 protein, p21 protein, p27 protein, and other protein kinases in mitigating colorectal cancer, lung cancer, oral cancer, squamous cell carcinoma, cervical cancer, breast cancer, bladder cancer, and prostate cancer, are reviewed and discussed in the report of Rady et ah, “Cancer Preventive and Therapeutic Effects of EGCG, the Major Polyphenol in Green Tea,” Egyptian Journal of Basic and Applied Sciences, 5 (2016) 1-23. These reports discuss the medical benefits of EGCG catechin polyphenols.
  • EGCG is stable during transit through the intestines when it is taken orally. After it is absorbed by the intestines, EGCG becomes metabolized by intestinal cells. Digested catechins such as EC and EGCG tend to produce glucuronic acids and then may also become partially methylated, forming 3-O-methyl groups. What this means is that oxidized catechins are an attractor for methylating processes and will remove and chemically incorporate the methyl groups from endogenous methylating agents. DNA methylation is strongly implicated in one of the causes leading to the development of cancers.
  • oxidative methylation metabolites of the polyphenolic catechins might be useful to mitigate other methylation dysfunctions that are often associated with the randomization of the epigenome and therefore the aging process. Cancer is only one of the many possible cumulative errors that can lead to somatic as well as neurological disease states. It is important to note that methylation antagonists are one area of scientific medical research. Therefore, it is widely appreciated that the medical usefulness of the catechins and of EGCG requires more focus and innovation, and that cancer prevention is likely not the only medical purpose to which this material or its derivatives will find new application in the future, as the association of EGCG in combination with other molecules has been found synergistic with combating cognitive diseases and is now gaining considerable attention.
  • EGCG binds directly to tumor suppressor protein molecule p53.
  • EGCG stabilizes the p53 protein in human cells by promoting the phosphorylation of serine residues leading to cell growth arrest.
  • the p53 protein is a DNA transcription factor when it is in the cell nucleus. When p53 becomes somatically mutated, it also becomes unable to signal the performance of DNA repair processes at the nucleus of cells in more than half of all known cancers.
  • P53 is normally maintained at low concentration in healthy cells by ubiquitylation and subsequent degradation, unless or until it is needed. When activated in response to sufficient cellular stress arising from local dysfunctions, p53 promotes cell-cycle arrest or programmed cell death (apoptosis) when it is in the cytoplasm or in mitochondria. These dysfunctions may include oxidative stress, and DNA damage leading to cell proliferation forming tumors and possibly also cancers.
  • NTD N-terminal domain
  • the NTD of tumor suppressor protein p53 is an intrinsically di sordered protein that normally interacts with many signaling proteins including the regulatory E3 ligase MDM2.
  • the reaction of any protein with uhiquitin is a signal to the proteasome that it is marked for degradation and catabolism.
  • the function of MDM2 is to expedite the ubiquitylation and subsequent degradation of p53 when it is not needed to confer programmed cell death for senescent cells.
  • Some small molecules can inhibit the ubiquiti nation of p53, thereby leaving it available and active for anti-tumor service.
  • the hydrophobic side of an a-helix in NTD contains a centrally located tryptophan residue W23 that is thought to be most easily disrupted by a small molecule that can bind directly to this region.
  • the extreme N terminus of p53 is a cation-pi interaction of the indole ring of tryptophan residue 91 with a proximal coplanar guanidinium group of arginine residue 174 at a vertical distance of 0.36 nanometers.
  • These amino acid residues are surrounded by numerous carboxylic acid functional groups that assist in retaining the open and disordered NTD conformation in the cytosol, therefore it is logical that an acidic molecule will have the best chance of forming an interaction at or about the amino acid residue of tryptophan 91 in the manner of MDM2, thereby preventing MDM2 from docking at this location.
  • EGCG can bind with the NTD of p53 to disrupt the p53-MDM2 interaction.
  • EGCG has been reported to disrupt the MDM2 and p53 interaction in human lung cancer cells, which resulted in the inhibition of p53 ubiquitylation and subsequent degradation.
  • the effect of EGCG is to increase the overall cellular concentration of P53 and the ability to cause the apoptosis effect.
  • An acidic p53 binding substance must be able to penetrate cell lipid membranes to render protection against oxidative and nitrative stress influencing the genes responsible for producing p53 in the cell nucleus.
  • Some of the current therapeutic strategies have put emphasis on the design of multiple functional properties into molecules or particles that enable them to target enzymes or receptors to help correct the dysfunctions leading to cancer disease states. Of these, none express the correct combination of lipid membrane permeability and acidic hydrophilic character to operate on p53 proteins or to confer protection to the deoxyribonucleic acid (DNA) coding for it, in any combination.
  • a significant limitation to the use of dietary EGCG is in the lack of maturity of cell signaling designs. Such design failures are attributed to an incomplete understanding of cell signaling functions and protein messaging effects.
  • Cell signal interactions begin with surface charges at membranes. Surface charges are in contact with the cell cytosol, proteins, DNA, and the lipid membranes of the cell. Some signaling regions, such as at the site of endoplasmic and sarcoplasmic reticula of mitochondria within tumors, may become insufficiently engaged in oxidation that is associated with the development of surplus glycolysis and waxy, hydrophobic proteins networked with excess glutathione and other sulfur containing molecules.
  • such a treatment should include a means to remove sources of free radicals even under reducing conditions, to include a very localized and very targeted acidic functionality while also retaining lipid membrane permeation ability. It is believed the present invention provides the first broadly effective discovery of such a composition, having a biological and electrochemical design to confer multiple therapeutic and prophylactic functions to highly targeted p53 protein structures, and especially affecting DNA in the cell nucleus. This composition will change our perspective on applications to boost resistance to the effects of cancer. The use of different carrier formulations enables appropriate methods of administration for this novel composition.
  • This invention is a composition of unique fullerene poly digalloyl nanoparticles made from commercially available buckrmnsterfullerene, optional including groups of disodium phosphonate (FDSP), on reaction with a poly digalloyl such as penta-m-digalloyl-glucose or a catechin such as epigallocatechin gallate (EGCG).
  • FDSP disodium phosphonate
  • the present invention provides the first broadly effective discovery of a compound, having a biological and electrochemical design to confer multiple therapeutic and prophylactic functions.
  • the described carrier formulations, derivatives and compositions enable appropriate methods of administration and their use as a medicament, for example.
  • EGCG EGCG
  • the pendant acid phosphonates are neutralized with cations being preferably sodium to form di sodium phosphonate groups being of a surfactant nature and having a viral or fungal protease inhibiting function via the phosphonate sulfurization reaction.
  • the fullerene di sodium phosphonate group has the general formula (C60((OP(ONa) 2 )5) x -R y , with R is a selected catechin polyphenol or penta-m-digalloyl-glucose, for example.
  • R is EGCG.
  • y is 1 or 2
  • the derivate of buckminsterfullerene has the formula C60((OP(ONa) 2 ) 5 -C 22 H 18 O 11 or C60((OP(ONa) 2 ) 5 -C 22 H 18 O 11 ) 2 , respectively.
  • This fullerene derivative possesses properties which reflect the singular free radical scavenging chemical function of fullerene s, the anti-proliferative function of acidic catechin polyphenols, and the protease control function of cationic disodium phosphonates.
  • compositions ingress to penetrate waxy sulfurized proteins, to confer localized chemical quenching of excessive methylation, and to reduce the ubiquitination of p53 anti-tumor proteins critical to reducing and correcting DNA damage.
  • the catechin polyphenol group such as EGCG has anticancer properties.
  • the buckminsterfullerene derivative enhances or increases the pharmaceutical properties of the catechin polyphenol group.
  • the buckminster-fullerene group allows the regenerative oxidation and reduction of EGCG hydroxyl and carboxyl groups to moderate as an intermediary in the multiplicity of biological redox reactions. It is well known that redox reactions tend to take place at cellular membranes and especially at the internal membrane structures of cellular organelles.
  • Suitable FDSP cat ec Inins for use as a sacrificial methylation molecule and methylation antagonist include epicatechin (EC), epicatechin gallate (EGG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG) .
  • FDSP-EGCG is utilized as a methylating antagonist
  • FDSP-EGCG becomes sacrificially methylated at hydroxyl regions of the catechin functional group to control dysfunctional methylation because of the systemic aging process.
  • This process is enabled by polygalloyl quinic acid ester functional groups, where more functional groups provide a greater number of methylation sinks at the cost of less local reactivity but improved long term stability for the FDSP-catechins.
  • the FDSP-EGCG composition protects the epigenome from methylation induced aging by sacrificial methylation of FDSP -EGCG acting as a demethylating agent otherwise known as a methylation antagonist. This action protects the excessive chemical accretion of methyl (-CH3) functional groups on the epigenome to maintain proper gene expression critical for organism function.
  • FDSP-EGCG helps regulate epigenetic methylation mechanisms including crosstalk between DNA methylation, histone modifications and non-coding RNAs, and the methylation effects on gene expression. Specifically, FDSP-EGCG controls dysregulated methylation responsible disease progression in tumors and cancer cells. The extraction of methyl groups by sacrificial methylation of FDSP-EGCG therefore provides a pathway to avoid tumor and cancer cell generation.
  • FDSP-EGCG limits cognitive decline in neurological diseases.
  • levodopa can become methylated, resulting in the loss of function of the neurotransmitter dopamine that is metabolized from levodopa in the glutamate cycle, leading alpha synuclein plaque formation in the substantia nigra portion of the brain.
  • Alzheimer’s disease excessive methylation is quenched to limit the formation and agglomeration of beta amyloid plaques.
  • the FDSP-EGCG composition provides a demethylation property by sacrificial methylation of a pendant hydroxyphenyl group that is chemically activated by the presence of the C60 fullerene adduct.
  • the extraction of methyl groups by sacrificial methylation by FDSP-EGCG provides protection of neurotransmitters such as dopamine and its precursor levodopa from functional deactivation by methylation.
  • the sacrificial demethylation function of FDSP-EGCG acts to protect functional regions of p53 protein from methylation so that p53 can continue to perform repair work around DNA, as well as at tumor and cancer cells.
  • the FDSP-EGCG composition provides a desulfurization property by sacrificial oxidation of a pendant phosphonate group. Regions of excess sulfur arise from a local excess of glutathione, leading to hydrophobic sulfur-protein bonds associ ated with the waxy region that separates tumor cells from the native immune system. The extraction of sulfur from cross-linked and mis-folded waxy protein agglomerates by FDSP-EGCG leads to a unique mode of tumor and cancer cell penetration to better allow the natural immune response access to these types of cells.
  • the desulfurization function acts to free entrapped p53 protein which may become folded into and crosslinked with protein tangles associated with excessive glutathione in the regions around tumor and cancer cells.
  • FDSP-EGCG forms a complex with tp53 (p53) protein to avoid ubiquitin signaling tags that may otherwise reduce the effective lifetime of p53 DNA repair protein.
  • FDSP-EGCG bonds to the N-terminus of the p53 (also known as tp53) to prevent premature catabolism of this endogenous DNA repair protein.
  • Complexing FDSP-EGCG with the N-terminus of p53 is designed to block the well-known chemical association of p53 with double minute 2 homolog (DM2) also known as E3 ubiquitin-protein ligands. This prevents bonding of MDM2 to the transactivation domains of p53 at its tyrosine and tryptophan amino acid functional group residues.
  • DM2 double minute 2 homolog
  • the aromatic region of FDSP-EGCG is designed to generate highly effective aromatic pi bonds to the central docking location of the N-terminus at tryptophan residue number 91 to confer maximum p53 protein service lifetime.
  • the nanoparticle ensemble amplifies the well-known bacteriostatic effect of EGCG by the bond to C60, especially for those bacteria that are known as “super bugs” because they have evolved a resistance to prescribed antibiotics.
  • certain bacteria commonly live on the skirt of many people without causing harm. However, these bacteria can cause skin infections or buccal infections if they enter the body through cuts, open wounds, or other breaks in the skin.
  • a clear alternative to prescribed antibiotics for mouth, skin, or gastric infections by pathological strains of antibiotic resistant bacteria is provided.
  • Non-limiting examples of the types of bacteria that can be treated include methicillin-resistant Staphylococcus aureus (MRSA), group ‘A’ Streptococcus (GAS) or "strep” leading to ‘strep throat’, and Impetigo especially as it is most commonly found on the face as ruptured blisters that form a flat, thick, honey-colored (yellowish-brown) crust.
  • a nano- aerosolized composition carries the FDSP-EGCG in a carrier fluid dispenser, and the composition in gasified and delivered to the nose, mouth, trachea, and airways of a patient or user.
  • the FDSP-EGCG is adsorbed onto the pore structure of a mineral such as zeolite for oral administration and timed release into the intestinal tract wherein a variation of the silicon to aluminum ratio of this mineral, or a variation in the porosity of diatomaceous earth mineral, or like negative charged mineral, provides both a charged surface and different pore sizes and therefore a timed-release function.
  • a mineral such as zeolite for oral administration and timed release into the intestinal tract wherein a variation of the silicon to aluminum ratio of this mineral, or a variation in the porosity of diatomaceous earth mineral, or like negative charged mineral, provides both a charged surface and different pore sizes and therefore a timed-release function.
  • the FDSP-EGCG is formulated into a topical cream carrier for application to the skin and the buccal cavity regions.
  • the FDSP-EGCG is formulated into an oral solution with sweeteners, flavors, and preservatives suitable to formulate a beverage or to be used as an additive to existing beverages such as traditional tea or coffee.
  • FIG. 1 is an illustration of the molecular structures of two exemplary polyhydroxyl phenyl containing molecules, EGCG and tannic acid.
  • FIG. 2 is an illustration of the molecular structures of alternative flavan-3-ols, being additional exemplary catechin gallate raw materials suitable as substitutes for EGCG.
  • FIG. 3 is an illustration of the molecular structures of commercially available fullerene disodium phosphonates (FDSP).
  • FIG. 4 is an illustration of one pentagonal reacted portion of the molecular structure of fullerene disodium phosphonate (FDSP).
  • FIG. 5 is an illustration of the molecular structures for epigallocatechin gallate
  • FIG. 6 is an illustration of the molecular structures for epigallocatechin gallate
  • FIG. 7 is an illustration of a desulfurization reaction leading to tumor penetration and complex formation with p53 protein.
  • FIG. 8 is an illustration of C60-EGCG or FDSP-EGCG packed into the pores of substrates for timed release, such as Transcarpathian zeolite (clinoptilolite) or diatomaeeous earth.
  • FIG. 9 is an illustration of the method of synthesis of FDSP-EGCG formulated with solvents suitable for nano-aerosol administration.
  • FIG. 10 is an illustration of the method of synthesis of C60-EGCG and FDSP-EGCG formulated with flavors and preservatives suitable for water-based and solid based oral administrations.
  • FIG. 11 is an illustration of the method of creating FDSP-EGCG formulated with perfumes and thickeners suitable for topical skin administrations.
  • FIG. 12 is an illustration of personal administration of aspirated nano-aerosol containing the FDSP-EGCG nanoparticles.
  • FIG. 13 is an illustration of personal topical skin administration of FDSP-EGCG.
  • FIG. 14 is an illustration of experimental FTIR data for EGCG.
  • FIG. 15 is an illustration of experimental FTIR data for C60-EGCG.
  • FIG. 16 is an illustration of experimental FTIR data for C60-tannic acid.
  • FIG. 17 is an illustration of experimental FTIR data for FDSP.
  • FIG. 18 is an illustration of experimental FTIR data for FDSP-EGCG.
  • FIG. 19 is an illustration of experimental FTIR data for zeolite.
  • FIG. 20 is an illustration of experimental negative mode mass spectrograph data for buckminsterfullerene (C60).
  • FIG. 21 is an illustration of experimental negative mode mass spectrograph data for fullerene penta-di sodium phosphonate (FDSP).
  • FIG. 22 is an illustration of experimental negative mode mass spectrograph data for
  • FIG. 23 is an illustration of experimental negative mode mass spectrograph data for
  • FIG. 1 illustrates two of the most efficacious anti-cancer molecular structures 100 used in the present invention.
  • the molecular structure of epigallocatechin gallate (EGCG) 110 is the most thermally stable and therefore the best catechin used as a raw material to make the derivatives and formulations for the present invention, having chemical formula C 22 H 18 G 3 1 .
  • the bracketed region 120 of EGCG 110 is more generally known as the chromane or benzo dihydropyran functional group and is a common feature of the acceptable catechin polyphenols in this composition.
  • An acceptable replacement for EGCG 110 is the polygalloyl quinic acid esters with the number of galloyl moieties per molecule ranging from 2 up to 12, is represented by the tannin molecular structure 130 for tannic acid, also known as penta-m-digalloyl-glucose. Tannic acid is reproducibly about equal in anti-proliferative and anti -bacterial function to the efficacy of EGCG, which points to both EGCG 110 and tannic acid 130 as ideal candidates for development as functional groups to enhance their anti-cancer properties because of each expresses di -hydroxy phenyl or tri-hydroxy phenyl (more generally, polygalloyl) functionality in their outer molecular structures.
  • Substances 110, 130 are used to help create, process, or deliver parts of the composition or their metabolites according to these teachings.
  • FIG. 2 is an illustration of the molecular structures of flavan-3-ols being exemplary catechins 200 that are acceptable substitutes for EGCG and may be independently selected from the flavan-3-ol group also known as catechins, or physiological metabolites thereof, to be reacted to produce the composition of the present invention.
  • These benzo-hydropyran moieties provide at least one of a tri-hydroxyphenyl group or a di -hydroxy phenyl group of the composition.
  • the EGCG catechin may be economically desirable to replace the EGCG catechin with an alternative thermally less stable benzo-hydropyran, such as the catechin having formula C 15 H 14 O 6 (EC) 210, or the catechin having formula C 15 H 14 O 7 (EGC) 220, or the catechin having formula C 22 H 18 G 1 0 (ECG) 230, or catechin formula C 15 H 14 O 6 (Robinetinidol) 240.
  • the three molecules 220, 230, 240 are experimentally well known to be more reactive polyphenols and can be used to increase the potency of the composition to make it more antimicrobial for some methods of use, such as for treating periodontal disease, than the di-hydroxylated catechin 210.
  • the catechin properties become greater and their chemical reactivities become significantly magnified then they are reacted as adducts to the fullerene disodium phosphonates (FDSP), according to the teachings of the present invention.
  • FDSP fullerene dis
  • FIG. 3 illustrates two alternative side view's of the molecular structures for fullerene di sodium phosphonates (FDSP) 300.
  • the commercial product FDSP is a core C.60 fullerene 310, 320 that is covalently bonded with five phosphonate groups 330, 340, 350, 360, 370, which can also be illustrated collectively as shown by the bracketed region 380.
  • the FDSP used as a reactant to produce the FDSP catechin composition(s) of this invention can be obtained commercially.
  • FIG. 4 illustrates a portion of a front view of a central pentagonal carbon region of the buckmiiisterfullerene (C60) molecular structure of FDSP 400.
  • FIG. 5 illustrates the molecular structures for the epigallocatechin gallate (EGCG) reaction with buckminslerfullerene (C60) 500.
  • the exemplary catechin, epigallocatechin gallate (EGCG) 510 is combined to react with buckminsterfullerene (C60) 520 in a reactive shear mill under conditions of shear mixing and pressure.
  • a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 and simultaneously shift the density of states of the electrons of the carbon cage into anisotropic electrostatic distributions.
  • FIG. 6 illustrates a chemical reaction to form a C60-EGCG 600.
  • the exemplary catechin, epigallocatechin gallate (EGCG) 610 is combined to react with fullerene disodium phosphonate (FDSP) 620 in a reactive shear mill under conditions of shear mixing and pressure.
  • a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 of the FDSP and simultaneously shift the density of states of the electrons of the carbon cage into anisotropic electrostatic distributions.
  • These electrostatic charges then achieve a metastable state when abutted proximal to simultaneously induced opposing electrostatic charges with at least one catechin gallate, EGCG.
  • FDSP-EGCG contains a multiplicity of diphosphonate functional groups that may disassociate at least one migrating sodium ion 630 to leave at least one corresponding pendant negative charged anionic phosphonate 640 capable of forming a hydrogen bond 650, illustrated as a dotted line, with any hydroxyl group of the EGCG structural region.
  • the cationic sodium is then able to form a pi-cation bond 660 with any aromatic region of the core C60 functional group 670 of EGCG-FDSP.
  • At least one aromatic region of the EGCG molecular structure 680 may form a pi-carbonyl bond 690 with the C60 functional group to significantly stabilize the resulting molecular ensemble.
  • Any carbonyl functional group of EGCG can create a pi-carbonyl bond 695 with an aromatic region of the C60 functional group to further stabilize the EGCG-FDSP molecular structure. It is to be understood that any combination of the catechin gallates specified herein are acceptable substitutions for the EGCG molecules in the reaction with FDSP, according to the teachings of the present invention.
  • FIG. 7 illustrates a desulfurization reaction of FDSP-EGCG 700, leading to tumor penetration.
  • the core tetramer of the endogenous DNA repair protein p53 is represented herein as a schematic geometric form 710.
  • the complete structure of the p53 protein with all amino acid residues is available at the protein data bank under rcsb.org/stmcture/2XWR.
  • the context of a p53 core tetramer bound to an arbitrary region of DNA being repaired is publicly available under reference code 3EX.J under rcsb.org/structure/3EXJ.
  • the EGCG is provided with aromatic pi to carbonyl bonds 730, and with aromatic pi to aromatic pi stacking bonds 740, 750 to the C60 functional group as shown by dashed lines in this molecular structure.
  • the presence of phosphonate groups of oxidation state three is provided in the molecular structure of FDSP-EGCG to penetrate the sulfur-rich hydrophobic region of tumors and metastasized cancer cells, as well as to desulfurize fungal proteases associated with respiratory pathology such as valley fever, or to desulfurize viral proteases.
  • the phosphonate sulfurization reaction proceeds by extraction of sulfur (S) as indicated by the black arrow 750, where the source of extracted sulfur can be a local excess of glutathione and sulfur-protein bonds associated with the waxy region that separates tumor cells from the native immune system carried by aqueous phase physiological plasma such as blood in the circulatory system.
  • One of the phosphonate groups of FDSP-EGCG is sulfurized by the acquisition of a sulfur atom 760.
  • the sulfurization results in a sulfurized phosphonate having phosphorus of oxidation state 5.
  • Sulfurization demonstrates the superiority of FDSP-EGCG over C60-EGCG in penetrating the regions where p53 was rendered inactive by protein misfolding and entrapment by sulfur bonded protein regions.
  • a primary intended result of freeing p53 from protein entrapment by sulfur adducts is to further extend the service life and anti-tumor function of p53 by the facile hydrogen bond association of the acidic hydroxyl groups of the EDSP-EGCG with the disordered and splayed hydroxyl groups of the N -terminal region of p53 proteins. This is achieved by complexing FDSP- EGCG with the N- terminus of p53 to block the chemical association of DM2 to the transactivation domains of p53, being localized to a few tyrosine and tryptophan amino acid functional group residues.
  • the DM2 bound to the transactivation domain of p53 (not shown) is publicly available at rcsb.org/structure/lYCR.
  • the aromatic region of FDSP-EGCG is complexed at the central docking location of the p53 N-terminus (medically known to be at or about the amino acid residue number 91 for tryptophan) by means of aromatic pi-pi bonding to the C60 functional group 780 thereby significantly stabilizing this complex and improving the DNA repair function of p53 beyond that expected for the unreinforced p53 protein in its natural state without a FDSP-EGCG complex.
  • the remaining four disodium phosphonate groups 790 may continue to act as desulfurization agents, as these can provide additional subsequent desulfurization reactions, thereby enabling p53 protein complexed with FDSP-EGCG to penetrate even more deeply into the waxy sulfurized coatings around tumor and cancer cells, according to the teachings of the present invention.
  • FIG. 8 illustrates the porous substrate zeolite or diatom aceous earth impregnated with C60-EGCG or FDSP-EGCG 800.
  • Transcarpathian zeolite (clinoptilolite) 810 is a type of mineral provided with a highly negatively charged network structure achieving a system of reproducible and well-defined pores and channels.
  • Clinoptilolite zeolite Is well known to adsorb nitrogen containing compounds including ammonia, amino acids, and other positive charged molecules.
  • Clinoptilolite zeolite is optionally used herein to adsorb thiamine (vitamin Bl) as positive counter-ion and hydrogen bonding adduct.
  • the thiamine adducts can be used to stabilize the impregnation with the composition of FDSP-EGCG in the form of a multiplicity of clusters 820, 830, 840, 850, 860, and 870 having cluster sizes sufficiently small to fit within the pore regions, being greater than 100 nanometers and less than about 5 microns in size. It is also known that at pH greater than 7, as well as under saline or physiological ionic salt conditions, clinoptilolite zeolite displaces and expresses the positively charged nitrogen compounds and counterions stored within the pores. The salt and pH moderated regenerant property of clinoptilolite towards reversible expression and release of positively charged nitrogen compounds has led to the widespread economic commercial adoption of clinoptilolite Transcarpathian zeolite as a dietary supplement.
  • Diatomaceous earth is a silicate bearing mineral composed of a multiplicity of silicon dioxide skeletons of diatoms having a multitude of shapes 880, 885 and being from 30 to about 200 nanometers in size.
  • the negatively charged diatomaceous silicates can adsorb thiamine (vitamin Bl) as a positive counter-ion and hydrogen bonding adduct; these adducts stabilize the impregnation with the composition of FDSP-EGCG in the form of a multiplicity of clusters 890, 895.
  • diatomaceous earth slowly diffusion releases and expresses FDSP-EGCG and thiamine counterions stored within the pores and the spaces between the silicate structures of the diatoms to achieve a timed-release of the FDSP-EGCG composition into the digestive tract.
  • Transcarpathian zeolite (clinoptilolite) or diatomaceous earth or other solid pharmaceutical grade minerals
  • Transcarpathian zeolite clinoptilolite
  • diatomaceous earth or other solid pharmaceutical grade minerals may be used as adjuvant delivery or timed-release delivery in any combination whatsoever, to perform timed digestive release of the composition of the present invention as one method of oral delivery of the composition of the present invention, according to these teachings.
  • FIG. 9 is a flowchart representation of an exemplary scalable synthesis method S900 of nano-aerosol FDSP-EGCG formulated for nano-aerosol administration.
  • step S910 To one mole of FDSP, add 5 moles of a catechin; EGCG is the preferred catechin.
  • step S920 the prepared dry powder mixture is reaction shear milled at about 55 °C to achieve the desired FDSP- EGCG product whereby a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 of the FDSP and simultaneously shift the density of states of the electrons of this cage molecule into anisotropic electrostatic distributions that achieve a metastable state when abutted to simultaneously induced opposing electrostatic charges with least one abutting proximal EGCG polyphenol.
  • step S930 the desired concentration of FDSP-EGCG is created by dissolving a weighed amount of the dry powder into a 70% glycerol and 30% polypropylene glycol solvent mixture by volume, in step S940, a metered amount of the nano aerosol fluid from step S930 is generated by a commercially available electronic dispensing device suitable for client inhalant aspiration by means of a heated airflow between about 255 °C and 300 °C to create the nano-aerosol, according to the teachings of the present invention.
  • FIG. 10 is a flowchart representation of an exemplary scalable method S1000 for synthesis of oral administered FDSP-poly galloyl.
  • step SI 010 about 1 mole of commercially available FDSP is combined with nominal 5 moles of a catechin, EGCG or tannic acid.
  • step S1020 the combined mixtures are milled at about 1000/sec shear rate and about 55 °C to achieve the desired FDSP-EGCG reaction product.
  • a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 of the FDSP and simultaneously shift the density of states of the electrons of this cage molecule into anisotropic electrostatic distributions that achieve a metastable state when abutted to simultaneously induced opposing electrostatic charges with least one abutting proximal EGCG polyphenol.
  • the FDSP-EGCG from step 1020 is mixed into food grade slow-release solid carrier material such as a Transcarpathian zeolite (clinoptilolite), diatomaceous earth, or like porous solid phase.
  • step S1040 the desired concentration of FDSP-EGCG is created by dissolving a weighed amount of the dry powder mixture with the porous scaffold component into a mold for pressing into an oral tablet. Alternatively, a weighed dosage of this power mixture is filled into capsules to be administered for oral administration of the time-release formulation.
  • This serving size or dosage may then be dispersed into any amount of water if desired, prior to oral administration, to ease consumption. It is understood that such a water dispersion of the time-release formulation is unstable and subject to settling on standing for periods of greater than a few hours.
  • this formulation may be dispensed into aqueous media for later distribution at any time for later oral administration, with the provision of optional viscosity modifiers that can be added to this mixture to stabilize the insoluble mineral components from settling therein as a minor variation to this method. This enhances the long-term esthetic appeal of the solid dispersed into an aqueous medium, while simultaneously maintaining the time-release feature of the porous solid insoluble carriers, according to these teachings.
  • FIG. 11 is a flowchart representation of an exemplary scalable method S 1100 for producing and applying a topical skin or buccal administered FDSP-catechins.
  • step S1110 To one mole of FDSS , add 5 moles of a catechin as in the preferred EGCG.
  • EC epicatechin
  • ECG epicatechin gallate
  • ECC epigallocatechin
  • step S1120 the mixture of step S1 110 is reaction shear milled at about 55°C to achieve the desired FDSP reaction product.
  • a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the 6C0 of the FDSP and simultaneously shift the density of states of the electrons of this cage molecule into anisotropic electrostatic distributions that achieve a metastable state when abutted to simultaneously induced opposing electrostatic charges with least one abutting proximal catechin polyphenol such as EGCG.
  • the product of Step 1120 is dissolved into water.
  • about 1% to 2% of the product is added to hyaluronic acid, along with about 4% perfume, a desired amount of methacrylic acid for viscosity enhancement, and 1% preservative.
  • step SI 140 the pH of the acidic FDSP-EGCG composition is adjusted to prevent mold or bacterial growth with an acceptable range of 5 to 6.7 and a nominal value of 6.5 by neutralization with sodium hydroxide (NaOH) with adequate mixing to ensure a uniform cream or lotion.
  • this material composition is transferred into beauty and cosmetic cream jars or tubes having a sufficiently hermetic seal to able to retain the volatile aromas or flavorings.
  • step 1160 the face is washed to remove natural skin residues prior to applying the topical formulation, such as before bedtime.
  • the buccal formulation can be applied, for example, after brushing the teeth.
  • FIG. 12 illustrates a method SI 200 for the personal administration of aspirated nano-aerosol delivery of fullerene penta-di sodium phosphonate epigallocatechin gallate (FDSP- EGCG).
  • the nano-aerosol generating device 1210 filled with FDSP-EGCG dispensing solution is provided for dispersing and nebulizing the inhalant gas including the nano-particles.
  • the device 1210 may also be more commonly known as a nebulizer, or an electronic vaporizing device, or an electronic cigarette, or the functional part of a hookah to be shared among several users.
  • these systems serve to carry the FDSP-EGCG in a carrier fluid dispenser 1210, and to transfer that composition in nebulized form along with an aerosolized solvent in a substantially gaseous dispersion to the nose, mouth, trachea, and airways of a patient or user 1220.
  • a carrier fluid dispenser 1210 serves to carry the FDSP-EGCG in a carrier fluid dispenser 1210, and to transfer that composition in nebulized form along with an aerosolized solvent in a substantially gaseous dispersion to the nose, mouth, trachea, and airways of a patient or user 1220.
  • One intended use of the FDSP-EGCG composition is to treat, delay or arrest the incidence of cancers wherein the nano-aerosol can expedite targeted delivery to the brain by avoiding a passage through the digestive system.
  • nano-aerosolized composition is exhaled and shown as particulate clusters 1230, 1240, 1250 within exhaled smoke puffs 1260 and 1270 emitted on exhalation as indicated by the direction of thin line arrows radiating away from the nose of the subject 1220.
  • Delivery of the C60-FDSP-EGCG nano-aerosol composition from dispenser 1210 provides antioxidant properties to the mucus airway tissues wherein destruction of free radicals and oxidants associated with motor neuron disease and Parkinson’s disease are part of the treatment and alpha-synuclein plaque mitigation is provided.
  • Systems that may be used for the method of dispersion of the FDSP-EGCG represented by dispenser 1210 include, without limitation, any of the electronic cigarette devices produced internationally and listed in Appendix 4.1, '‘Major E- cigarette Manufacturers” of the “2016 Surgeon General's Report: E-Cigarette Use Among Teen and Young Adults” published by the Center for Disease Control and Prevention (CDC), Office of Smoking and Health (OSH) freely available at the CDC.GOV website, or any combination of piezoelectric, resist] vely heated, or inductively heated vaporized fluid delivery methods that can be utilized to deliver the composition of the present invention, especially when approved as a medical drug delivery device.
  • CDC Center for Disease Control and Prevention
  • OSH Office of Smoking and Health
  • Each embodied variation of such methods without limit are intended to aspirate aerosols as the method of therapeutic substance delivery of the composition of the present invention directed into the nasal cavities, mouth, tracheal breathing orifice, or intubated trachea of a patient.
  • the supply direction of nebulized feed of FDSP-EGCG on inhalation and exhalation are delivered into the airways and lungs of the intended patient by the flow' of supplied air as indicated by the direction of upward and downward facing large white arrows 1280, when used according to these teachings.
  • FIG. 13 illustrates personal topical skin administration and buccal administration of fullerene penta-(di sodium phosphonate) epigallocatechin gallate (FDSP-EGCG) composition 1300.
  • a semi-liquid slurry dispersion, cream, ointment, or lotion can he used to contain and transfer the administered formula, as a somewhat different formulation is required depending on whether the application is for topical use on the skin or to coat tooth surfaces in the oral or buccal cavity, which is located inside the mouth 1310.
  • the skin care formula can he applied by the user 1320 in regions such as the face 1330, 1340.
  • Application of the skin care formulation can be by means of circular rubbing motions as indicated by the direction of arrows 1350, 1360.
  • the skin- care formulation then confers topical antimicrobial properties such as for MRS A and other antibiotic resistant skin infections, anti-aging and skin brightening functions, and to promote resistance to the onset of skin cancers.
  • topical antimicrobial properties such as for MRS A and other antibiotic resistant skin infections, anti-aging and skin brightening functions, and to promote resistance to the onset of skin cancers.
  • the oral mucosal antibacterial functions of the nanoparticle ensemble are to promote anti-gingivitis properties, such as to treat periodontal disease, especially for those bacteria that are known as “super bugs” because they have evolved a resistance to prescribed antibiotics, as well as to promote anti-esophageal cancer treatment properties.
  • Non- limiting examples of the type of bacteria that can be treated include methicillin-resistant Staphylococcus aureus (MRSA), group ‘A’ Streptococcus (GAS) or "strep” leading to ‘strep throat’, and Impetigo especially as commonly found on the face in the form of ruptured blisters that form a flat, thick, honey-colored (yellowish-brown) crust.
  • MRSA methicillin-resistant Staphylococcus aureus
  • GAS group ‘A’ Streptococcus
  • strep leading to ‘strep throat’
  • Impetigo especially as commonly found on the face in the form of ruptured blisters that form a flat, thick, honey-colored (yellowish-brown) crust Impetigo especially as commonly found on the face in the form of ruptured blisters that form a flat, thick, honey-colored (yellowish-brown) crust.
  • FIG. 14 illustrates experimental FTIR data for EGCG raw material
  • the sample was prepared by the method of mixing, crushing, and consolidating under 7 metric tons of pressure, about 0.001 grams of analyte with 1 gram of a diluent solid material that is substantially transparent to infrared light, this diluent being anhydrous potassium bromide (KBr), which then flows under pressure to form a translucent pellet of about 0.4 mm thickness.
  • Spectral background subtraction in air using a control pellet of the same mass and thickness having pure KBr was used to obtain a baseline instrument infrared spectral transmission response.
  • This method is generally referred to as the ‘KBr pellet’ sample preparation method, and it is used hereinafter throughout for each FTIR experimental data collection and spectral analysis.
  • the sample of EGCG analyte prepared by KBr pellet obtains a broad characteristic absorbance from 3100 cm -1 to 3600 cm -1 arising from hydroxyl (OH) functional groups bonded to each aromatic ring.
  • the absorbance at 1450 cm -1 arises from the C-H group present in the Chromane ring.
  • FIG. 15 illustrates experimental FTIR data for C60-EGCG. A very strong and sharp
  • C60 fullerene aromatic carbon-carbon (C-C) stretching band appears at 576 cm 4 and, and a less intense but also sharp carbon-carbon absorbance appears at 526 cm -1 .
  • Constrained carbon- hydrogen stretching bands appear at 2921 cm -1 and 2851 cm -1 attributed to the likely interaction of the EGCG ring structures with the fullerene ring structure through aromatic pi bonding.
  • the pure EGCG carbonyl absorbance at 1646 cm -1 shown in FIG. 14 is now seen to be decreased in intensity and shifted to 1684 cm -1 ; however, the pure EGCG carbonyl absorbance at 1609 cm -1 shown in FIG. 14 is verifiably identical in intensity and remains at 1609 cm -3 .
  • FIG. 16 illustrates experimental FTIR data for C60-tannic acid. Characteristic sharp
  • C60 fullerene aromatic carbon-carbon stretching bands appear at 526 cm -1 and 576 cm -1 .
  • the central glucose molecule makes strong carbon-oxygen vibrational contributions at 1197 cm -1 and 1318 cm -1 .
  • the broad absorbance region from 3680 cm -1 to about 2870 cm -1 is attributed to the hydroxyl functional groups of the poly-galloyl structures in this molecule.
  • FIG. 17 illustrates experimental FTIR data for fullerene disodium phosphonate
  • FIG. 18 illustrates experimental FTIR data for fullerene disodium phosphonate - epigallocatechin gallate (FDSP-EGCG).
  • FDSP-EGCG fullerene disodium phosphonate - epigallocatechin gallate
  • This effect is attributed to the change of the EGCG hydroxyl group stretch from being dissimilar in two regions with respect to planar phenolic symmetry to a uniformly constrained hydroxyl group stretch that is geometrically dominated by the proximal phosphonate groups at their distal ends, and the proximal fullerene with pi-pi bonds appending to each aromatic phenyl group of the EGCG, wherein these combined effects indicate a strongly held and partially wrapped configuration.
  • This design feature shows that both the geometry as well as the acidity of the EGCG functional moiety, according to these teachings.
  • FIG. 19 illustrates experimental FTIR data for the natural Transcarpathian zeolite
  • the absorbances at 2918 cm -1 and 2850 cm - 1 arise from trace carbon hydrogen stretching of organic materials adsorbed onto the structure of the natural zeolite.
  • the absorbances at 1634 cm -1 and 1574 cm -1 are attributed to two different types of steric environments associated with hydroxyl (OH) bending vibrations.
  • the absorbance peak at 1455 cm -1 is attributed to adsorbed amine contributing to a signal of nitrogen-hydrogen bending (-NH) which correlates with the absorbance at 3626 cm -1 for nitrogen-hydrogen (NH) stretching vibrations.
  • the very intense and broad beak at 1031 cm -1 has a characteristic shoulder absorbance at 1197 cm -'1 which collectively characterize the primary absorbance patterns of zeolite arising from its aluminum-oxygen (AIO) and silicon-oxygen (SiO) bending vibrations, where the position of this band depends on the aluminum to silicon ratio and determines the number of the A1 atoms per formula unit.
  • AIO aluminum-oxygen
  • SiO silicon-oxygen
  • the asymmetric stretching due to the internal vibrations of the zeolite silicon-oxygen (SiO) framework tetrahedra occurred at 790 cm -3 .
  • the symmetric stretching due to the internal vibrations of silicon- oxygen (SiO) framework tetrahedra is attributed to the absorbance peak at 719 cm -1 .
  • FIG. 20 illustrates experimental negative mode MALDI-TOF mass spectrograph data for Buc kminsterfull eerene (C60).
  • This sample as well as each of the subsequent MALDI- TOF experimental test results hereinafter, was introduced for test by laser vaporization into a Voyager Mass Spectrograph from Applied Biosystems (Foster City, California, USA).
  • Negative mode bombardment was by fast moving electrons at about 70 eV energy. This resulted in molecular fragmentation and electron removal from the highest molecular orbital energy as molecular ions were formed.
  • the ratio of mass to charge (rn/z) is used to determine the molecular ion fragments to help determine the pieces of the original molecule in this assay.
  • the mass peak at 720 m/z corresponds to the molecular ion of fullerene C60.
  • the overall experimental test results characterize the molecular ion breakdown product of buckmlnsterfullerene (C60), where C60 may be used to further synthesize the composition of the present invention.
  • FIG. 21 illustrates experimental negative mode MALDI-TOF mass spectrograph data for FDSP.
  • Negative mode bombardment was by fast moving electrons at about 70 eV energy.
  • the mass peak at 720 m/z corresponds to the molecular ion of fullerene C60 functional group.
  • the large number of sharp peaks with a cluster maximum at about 1967 m/z are attributed to the spallation products of partially ablated disodium phosphonate functional groups.
  • the overall experimental test results characterize the molecular ion breakdown products of FDSP, where FDSP may be purchased commercially or can be synthesized as explained herein and then is to be used to further synthesize the composition of the present invention.
  • FIG. 22 illustrates experimental negative mode MALDI-TOF mass spectrograph data for C60-EGCG.
  • the appearance of a multiplicity of spikes having separation of mass to charge ratio of 24 are attributed to the loss of dicarbide ions (C-C) associated with the presence of pi-pi aromatic bonds.
  • the primary peak at about 726 mass to charge ratio is attributed to the fullerene functional group of 720 m/z having 6 adducts of hydrogen, and the cluster of peaks at about 1443 m/z are attributed to the presence of non-covalent pi-pi intercalation of EGCG, some of which may be shared between dimeric fullerene functional groups.
  • the minor trace of peaks above this mass may indicate some traces of multimeric fullerene chains with signals below a threshold that is useful for interpretation and analysis.
  • the characteristic mass spallation patterns are consistent with and representative of the formation of C60-EGCG for this component of the composition according to the teachings of the present invention.
  • FIG. 23 illustrates experimental negative mode MALDI-TOF mass spectrograph data for FDSP-EGCG.
  • the mass peak at 723 m/z corresponds to the molecular ion fragment of fullerene C60 adduct with three residual hydrogen atoms, a unique feature of the main molecular spallation ion of this product.
  • the complicated sharp rider peaks are attributed to the mass fragments of phosphonates as these disassemble from the base fullerene group.
  • the peak at 1419 m/z is attributed to the presence of the non-covalent pi-pi intercalation of EGCG, some of which may be shared between dimeric fullerene functional groups, whereas the peaks centered at about 2042 m/z are attributed to the additional presence of covalently bonded disodium phosphonate groups which are also pendant from the fullerene functional group.
  • the characteristic mass spallation patterns for the illustrated MALDI-TOF data are consistent with and representative of the formation of FDSP-EGCG for this component of the composition according to the teachings of the present invention.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Inorganic Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pulmonology (AREA)
  • Zoology (AREA)
  • Nanotechnology (AREA)
  • Immunology (AREA)
  • Dermatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A nanoparticle composition of buckminsterfullerene with at least one poly galloyl functional group such as a catechin molecule, such as epigallocatechin gallate, or a tannic acid, is provided that is both anti-bacterial and efficacious to maintain or re-establish benign healthy cellular homeostasis. In addition, the ability to penetrate hydrophobic malignant tissues via desulfurization is promoted with the addition of phosphonate pendant groups. This further enables the composition to prevent or to treat chronic obstructive pulmonary disorder (COPD), to penetrate fungal spores, and to penetrate the hydrophobic regions of uncontrolled cellular proliferation, neoplasms, degenerative malignancy, as well as to help treat chronic inflammatory diseases associated with or leading to induce cancer in susceptible cells. The composition can be produced at low temperatures through reactive shear milling. Delivery methods include ingestion, topical application, topical buccal application, inhalation, or injection when used as a medicament or as a food supplement.

Description

FULLERENE PHOSPHONATE POLY DI-GALLOYLS AND METHODS
CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims the benefit of international application PCT/US22/12369 filed on January 13, 2022 and the benefit of United States provisional patent application 63/161,310 filed on March 15, 2021, both of which are incorporated herein by reference in their entireties,
BACKGROUND
1. FIELD OF INVENTION
[0002] The present invention is a composition of buckminsterfullerene with one or more polygalloyl functional groups, where these can be a quinic acid ester (catechin) or a benzo- hydropyran of at least one of a tri-hydroxyphenyl group or a di-hydroxyphenyl group and may also include disodium phosphonate groups to maintain or re-establish hormesis and healthy cellular homeostasis. One formulation of this composition is in topical beauty products to mitigate the cellular effects of skin aging. Another formulation of this composition combats bacterial infections such as periodontal disease, especially in the case of antibiotic resistant bacteria. The utility of this and other uses simultaneously and synergistieally is to prevent or help treat uncontrolled cellular proliferation, neoplasms, degenerative malignancy associated with cancers, and some chronic inflammatory diseases such as Alzheimer’s Disease, Parkinson’s Disease, and respiratory ailments such as chronic obstructive pulmonary disease (COPD), in susceptible cells. The provided delivery methods include ingestion, topical application, buccal application, inhalation, or injection. The composition can be formulated as a prophylactic medicament or used as a food supplement.
2. BACKGROUND ART
[0003] Green tea is recognized as the most effective cancer prevention drink in the world.
The cancer protection properties of various tea extracts are widely known and are clearly linked to a group of polyphenols of a type of flavanol derivatives, or flavonoids. These fiavonoids have a chemical structure known as a flavan-3-ol that each possess a 2 -phenyl-3, 4-dihydro-2H- chromen-3-ol skeleton. Of these fiavan-3-ols, there are four highly similar, structurally related catechin molecules that have been identified in green tea as being most able to prevent the development of cancer. These catechins are EC (epicatechin), epicatechin gallate (ECG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG). The similarity in both structure and nomenclature can sometimes lead to some confusion. In comparison medical studies, however, EGCG has been demonstrated to provide the most effective and prominent anticancer properties of each of these catechin variations, perhaps because the related less molecular weight substances are both more reactive and less thermally stable.
[0004] A different class of molecules also having benzo hydropyran groups are the tannins, which have at least one central glucose molecule as their distinguishing characteristic feature. Once benzene is derivatized with hydroxyl groups (OH) these groups are also known as phenyl groups. There are many types of tannins, and their structures may vary widely in the number of di-phenyl benzene and tri-phenyl benzene groups. Many of the tannins can become too large to be of effective use because of poor solubility, especially when multiple glucose groups become entrained into their structures. Of the family of tannins, one molecular member, a very simple one, called tannic acid or penta-m-digalloyl-glucose, has been found to be both low enough in molecular weight and sufficiently reproducible in structure to allow medical evaluation for anti- cancer and anti -bacterial properties. To date, medical studies and reports indicate that tannic acid is reproducibly about equal in anti-proliferative function to the efficacy of EGCG, which points to both EGCG and tannic acid as ideal candidates for future development to enhance their anti-cancer properties. It is thought that the anti-cancer properties in both EGCG and tannic acid arises because they express di-hydroxy phenyl or tri-hydroxy phenyl (more generally, polygalloyl) functionality in their outer molecular structures.
[0005] EGCG has a maximum solubility at room temperature of 92 grams per liter of water.
EGCG from green tea can induce apoptosis in cancerous cells. There is at most about 1 .25 grams per liter of EGCG in brewed green tea. Epidemiological studies indicate that a minimum of from 2.5 to 3 grams of EGCG ingested per day yields a blood serum concentration of 0.1-1 mM EGCG to obtain some decreased risk of cancers.
[0006] Several studies indicate that EGCG polyphenol confers beneficial effects against several (chronic) pathologies associated with oxidative stress damage to cells, Including multiple types of cancer, cardiovascular disease, and neurodegenerative diseases, in addition, EGCG provides significant antibacterial and antiviral properties that find use in Alzheimer’s disease. Reference is made to Sneideris et ah, “The Environment Is a Key Factor in Determining the Anti- Amyloid Efficacy of EGCG,” Biomolecules, 2019, 9, 855, and to Yang et al., “EGCG-mediated Protection of the Membrane Disruption and Cytotoxicity Caused by the ‘Active Oligomer’ of alpha-Synuclein,” Scientific Reports, 2017, 7, 17945, showing that EGCG inhibits growth of beta amyloid plaques associated with Alzheimer’s disease as well as plaques of alpha synuclein associated with Parkinson’s disease. Further, Mahler et al. (2020) AN euro protective Properties of Green Tea (Camellia sinensis) in Parkinson’s Disease: A Review,” Molecules, 2020, 25, 3926 show a similar effect of EGCG against Parkinson's disease by modulating several gene expressions and acting on mitochondrial REDOX signaling pathways. Anti-cancer properties of EGCG arising from activity on p53 protein, p21 protein, p27 protein, and other protein kinases in mitigating colorectal cancer, lung cancer, oral cancer, squamous cell carcinoma, cervical cancer, breast cancer, bladder cancer, and prostate cancer, are reviewed and discussed in the report of Rady et ah, “Cancer Preventive and Therapeutic Effects of EGCG, the Major Polyphenol in Green Tea,” Egyptian Journal of Basic and Applied Sciences, 5 (2018) 1-23. These reports discuss the medical benefits of EGCG catechin polyphenols.
[0007] EGCG is stable during transit through the intestines when it is taken orally. After it is absorbed by the intestines, EGCG becomes metabolized by intestinal cells. Digested catechins such as EC and EGCG tend to produce glucuronic acids and then may also become partially methylated, forming 3-O-methyl groups. What this means is that oxidized catechins are an attractor for methylating processes and will remove and chemically incorporate the methyl groups from endogenous methylating agents. DNA methylation is strongly implicated in one of the causes leading to the development of cancers. The oxidative methylation metabolites of the polyphenolic catechins might be useful to mitigate other methylation dysfunctions that are often associated with the randomization of the epigenome and therefore the aging process. Cancer is only one of the many possible cumulative errors that can lead to somatic as well as neurological disease states. It is important to note that methylation antagonists are one area of scientific medical research. Therefore, it is widely appreciated that the medical usefulness of the catechins and of EGCG requires more focus and innovation, and that cancer prevention is likely not the only medical purpose to which this material or its derivatives will find new application in the future, as the association of EGCG in combination with other molecules has been found synergistic with combating cognitive diseases and is now gaining considerable attention.
[0008] It is well known and characterized that EGCG binds directly to tumor suppressor protein molecule p53. EGCG stabilizes the p53 protein in human cells by promoting the phosphorylation of serine residues leading to cell growth arrest. The p53 protein is a DNA transcription factor when it is in the cell nucleus. When p53 becomes somatically mutated, it also becomes unable to signal the performance of DNA repair processes at the nucleus of cells in more than half of all known cancers.
[0009] P53 is normally maintained at low concentration in healthy cells by ubiquitylation and subsequent degradation, unless or until it is needed. When activated in response to sufficient cellular stress arising from local dysfunctions, p53 promotes cell-cycle arrest or programmed cell death (apoptosis) when it is in the cytoplasm or in mitochondria. These dysfunctions may include oxidative stress, and DNA damage leading to cell proliferation forming tumors and possibly also cancers.
[0010] N-terminal domain (NTD) has a disordered state that lacks folded structure and has an extended conformation with high flexibility and low binding affinity. A small molecule must be able to transform the NTD into a compact conformation of high molecular binding affinity. It is now' w'ell understood that the NTD of tumor suppressor protein p53 is an excellent target for anti-cancer effects in drug development by magnifying the NTD interaction with targeted small molecules to create a compact molecular complex, but the identity for such a design to be both efficient and efficacious is absent, and the way to create a targeted delivery is also absent, although there is much speculation and discussion about these topics to date.
[0011 ] The NTD of tumor suppressor protein p53 is an intrinsically di sordered protein that normally interacts with many signaling proteins including the regulatory E3 ligase MDM2. The reaction of any protein with uhiquitin is a signal to the proteasome that it is marked for degradation and catabolism. The function of MDM2 is to expedite the ubiquitylation and subsequent degradation of p53 when it is not needed to confer programmed cell death for senescent cells. Some small molecules can inhibit the ubiquiti nation of p53, thereby leaving it available and active for anti-tumor service. The hydrophobic side of an a-helix in NTD contains a centrally located tryptophan residue W23 that is thought to be most easily disrupted by a small molecule that can bind directly to this region.
[0012] Previous anti-tumor and cancer prophylaxis drug discovery attempts have aimed at disrupting p53-MDM2 interface at the NTD-binding pocket that is available to interact with the MDM2 surface. Binding of MDM2 is a well-known function of the NTD of p53, involving the global hydrophilic amino acid residues F19, L22, L26, and a centrally localized hydrophilic W23 tryptophan residue. These amino acid residues are proximally surrounded by numerous carboxylic acid functional groups and hydrophilic proline residues that assist in retaining the open and disordered NTD conformation in the cytosol. This information is available for public inspection at the x-ray crystal structures stored at the Protein Data Base (PDB) designated for 2XWR, showing the DNA-binding domain of human p53 with extended N terminus, available at rcsb.org/stmcture/2XWR. In like manner, the MDM2 bound to the transactivation domain of p53 is publicly available at https://www.rcsb.org/structure/ 1 YCR. For perspective, some have considered bonding the MDM2 protein ligase with another inhibitor to avoid interaction with p53, such as can be found by example at rcsb.org/structure/4ZFL
[0013] The extreme N terminus of p53 is a cation-pi interaction of the indole ring of tryptophan residue 91 with a proximal coplanar guanidinium group of arginine residue 174 at a vertical distance of 0.36 nanometers. These amino acid residues are surrounded by numerous carboxylic acid functional groups that assist in retaining the open and disordered NTD conformation in the cytosol, therefore it is logical that an acidic molecule will have the best chance of forming an interaction at or about the amino acid residue of tryptophan 91 in the manner of MDM2, thereby preventing MDM2 from docking at this location.
[0014] As a small acidic molecule with high complex formation ability, EGCG can bind with the NTD of p53 to disrupt the p53-MDM2 interaction. EGCG has been reported to disrupt the MDM2 and p53 interaction in human lung cancer cells, which resulted in the inhibition of p53 ubiquitylation and subsequent degradation. The effect of EGCG is to increase the overall cellular concentration of P53 and the ability to cause the apoptosis effect.
[0015] The excellent water solubility of EGCG at 92 grams per liter however limits the ability of this polyphenollc molecule to penetrate across lipid cellular membranes and into hydrophobic cellular compartments. This substantially explains the consistently high serving size or dosages of EGCG required to obtain some decreased risk of de veloping cancers by binding with p53 inside the cell cytoplasm or inside the cell nucleus, where transfer across cell membranes is essential to reach the p53 protein.
[0016] An acidic p53 binding substance must be able to penetrate cell lipid membranes to render protection against oxidative and nitrative stress influencing the genes responsible for producing p53 in the cell nucleus. Some of the current therapeutic strategies have put emphasis on the design of multiple functional properties into molecules or particles that enable them to target enzymes or receptors to help correct the dysfunctions leading to cancer disease states. Of these, none express the correct combination of lipid membrane permeability and acidic hydrophilic character to operate on p53 proteins or to confer protection to the deoxyribonucleic acid (DNA) coding for it, in any combination. [0017] While there is great promise in the beneficial effects of EGCG, there are no known strategies to enhance its poor stability against rapid metabolic breakdown to deliver this substance to the senescent cells where p53 production has been found to be mutated or deficient. Attempts to perform encapsulation within microspheres or nanoparticles or attempts to perform a molecular dispersion in polymer matrices to be used as carriers, have proven effective at delivery but remain insufficiently targeted to the ubiquitination site of protein p53 by current means.
[0018] In all cases, no present state of the art has considered to allow for the possibility of oxidative and reductive cycling of epigallocatechin gallate by tethering it to a specially designed reduction-oxidation (REDOX) center that is capable of both storing and releasing electrons and hydrogen protons for the purpose of recycling the active carboxyl groups of epigallocatechin gallate as an aid to the long-term maintenance of cellular redox homeostasis. Also, there are no present formulations that are clearly designed to ensure that the delivered, released, or free epigallocatechin gallate will be able to penetrate to and then remain at the active molecular signaling and biochemical redox site of the membranes of cell organelles. Part of the reason for this may be those current formulations use targeting that does not adequately provide simultaneous electrostatic and hydrophobic anchoring of epigallocatechin gallate to cellular membranes. Another reason for this may be the lack of a clearly defined function or mechanism to penetrate hydrophobic proteins, especially those containing sulfur-sulfur bridges.
[0019] A significant limitation to the use of dietary EGCG is in the lack of maturity of cell signaling designs. Such design failures are attributed to an incomplete understanding of cell signaling functions and protein messaging effects. Cell signal interactions begin with surface charges at membranes. Surface charges are in contact with the cell cytosol, proteins, DNA, and the lipid membranes of the cell. Some signaling regions, such as at the site of endoplasmic and sarcoplasmic reticula of mitochondria within tumors, may become insufficiently engaged in oxidation that is associated with the development of surplus glycolysis and waxy, hydrophobic proteins networked with excess glutathione and other sulfur containing molecules. This REDOX deficit is thought to contribute to dysfunction of the electron transfer cycle that allows proper cellular respiration to take place, and the result can be the production of misfolded proteins associated with tumorous or cancerous resistance to programmed cell death by apoptosis. Unfortunately, the present trend in the development of improved pharmaceutical and nutraceuticals that demonstrated limited control over cell signaling and has not been successful to improve the retention of p53 in combating the incidence of tumors and cancer. [0020] What is therefore needed is a novel therapeutic strategy or unique material used to confer cellular p53 protection and prevent, mitigate, or reverse toxic pathology arising from tumors or cancer related to p53 dysfunction before irreversible damage progresses. Desirably, such a treatment should include a means to remove sources of free radicals even under reducing conditions, to include a very localized and very targeted acidic functionality while also retaining lipid membrane permeation ability. It is believed the present invention provides the first broadly effective discovery of such a composition, having a biological and electrochemical design to confer multiple therapeutic and prophylactic functions to highly targeted p53 protein structures, and especially affecting DNA in the cell nucleus. This composition will change our perspective on applications to boost resistance to the effects of cancer. The use of different carrier formulations enables appropriate methods of administration for this novel composition.
SUMMARY OF THE INVENTION
[0021] This invention is a composition of unique fullerene poly digalloyl nanoparticles made from commercially available buckrmnsterfullerene, optional including groups of disodium phosphonate (FDSP), on reaction with a poly digalloyl such as penta-m-digalloyl-glucose or a catechin such as epigallocatechin gallate (EGCG).
[0022] It is an object to provide a compound that increases the bioavail ability, in particular the solubility in aqueous solutions, via the penetrability of hydrophobic regions, and the biological redox functionality of catechin polyphenols such as epigallocatechin gallate having the formula C22H18O11.
[0023] The present invention provides the first broadly effective discovery of a compound, having a biological and electrochemical design to confer multiple therapeutic and prophylactic functions. The described carrier formulations, derivatives and compositions enable appropriate methods of administration and their use as a medicament, for example.
[0024] Some embodiments of this Invention provide a cluster of nanoparticles composed with carbon fullerenes, optionally covalently derivatized with phosphonates having oxidation state of three, and a poly digalloyl such as a catechin moiety, being preferably EGCG, in which this substance is pi-carbonyl bonded from at least one carbonyl group (C=0) to the aromatic regions of the fullerene phosphonate. This enables particularly high solubility of the EGCG in aqueous solutions, thereby increasing the bioavailability of the EGCG and its therapeutic effect. [0025] The pendant acid phosphonates are neutralized with cations being preferably sodium to form di sodium phosphonate groups being of a surfactant nature and having a viral or fungal protease inhibiting function via the phosphonate sulfurization reaction. The fullerene di sodium phosphonate group has the general formula (C60((OP(ONa)2)5)x-Ry, with R is a selected catechin polyphenol or penta-m-digalloyl-glucose, for example. Preferably, R is EGCG.
[0026] In a preferred embodiment, y is 1 or 2, such that the derivate of buckminsterfullerene has the formula C60((OP(ONa)2)5-C22H18O11 or C60((OP(ONa)2)5-C22H18O11 )2, respectively. [0027] This fullerene derivative possesses properties which reflect the singular free radical scavenging chemical function of fullerene s, the anti-proliferative function of acidic catechin polyphenols, and the protease control function of cationic disodium phosphonates.
[0028] These properties allow the composition ingress to penetrate waxy sulfurized proteins, to confer localized chemical quenching of excessive methylation, and to reduce the ubiquitination of p53 anti-tumor proteins critical to reducing and correcting DNA damage.
[0029] In particular, the catechin polyphenol group such as EGCG has anticancer properties. By increasing the bioavailability of the poly digalloyl group through targeting redox reactions at cell membranes, the buckminsterfullerene derivative enhances or increases the pharmaceutical properties of the catechin polyphenol group. By provision of stored electrons or protons, in some embodiments, the buckminster-fullerene group allows the regenerative oxidation and reduction of EGCG hydroxyl and carboxyl groups to moderate as an intermediary in the multiplicity of biological redox reactions. It is well known that redox reactions tend to take place at cellular membranes and especially at the internal membrane structures of cellular organelles. This latter ability is highly promoted by the presence of the hydrophobic and lipophilic carbon facets of the buckminsterfullerene adduct, which is attracted to and then anchors In cell membrane lipids, as has been well documented in over 30 years of fullerene biochemical studies. The provision of, for instance, EGCG adducts to buckminsterfullerene thereby effectively targets the catechin polyphenol portion of this moiety to those biologically active sites at membrane surfaces where its activity will find the greatest cellular utility in moderating and regulating redox homeostasis where free radicals are most likely to collect and damage the integrity of the cell membrane.
[0030] In one aspect, multiple hydroxyl regions of the catechin functional group of FDSP becomes sacrificially methylated. Suitable FDSP cat ec Inins for use as a sacrificial methylation molecule and methylation antagonist include epicatechin (EC), epicatechin gallate (EGG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG) .
[0031] In a related aspect, FDSP-EGCG is utilized as a methylating antagonist, where
FDSP-EGCG becomes sacrificially methylated at hydroxyl regions of the catechin functional group to control dysfunctional methylation because of the systemic aging process. This process is enabled by polygalloyl quinic acid ester functional groups, where more functional groups provide a greater number of methylation sinks at the cost of less local reactivity but improved long term stability for the FDSP-catechins. Specifically, the FDSP-EGCG composition protects the epigenome from methylation induced aging by sacrificial methylation of FDSP -EGCG acting as a demethylating agent otherwise known as a methylation antagonist. This action protects the excessive chemical accretion of methyl (-CH3) functional groups on the epigenome to maintain proper gene expression critical for organism function.
[0032] In yet another related aspect, FDSP-EGCG helps regulate epigenetic methylation mechanisms including crosstalk between DNA methylation, histone modifications and non-coding RNAs, and the methylation effects on gene expression. Specifically, FDSP-EGCG controls dysregulated methylation responsible disease progression in tumors and cancer cells. The extraction of methyl groups by sacrificial methylation of FDSP-EGCG therefore provides a pathway to avoid tumor and cancer cell generation.
[0033] In yet another related aspect, FDSP-EGCG limits cognitive decline in neurological diseases. In Lewy body dementia and Parkinson’s disease, levodopa can become methylated, resulting in the loss of function of the neurotransmitter dopamine that is metabolized from levodopa in the glutamate cycle, leading alpha synuclein plaque formation in the substantia nigra portion of the brain. In Alzheimer’s disease, excessive methylation is quenched to limit the formation and agglomeration of beta amyloid plaques. The FDSP-EGCG composition provides a demethylation property by sacrificial methylation of a pendant hydroxyphenyl group that is chemically activated by the presence of the C60 fullerene adduct. The extraction of methyl groups by sacrificial methylation by FDSP-EGCG provides protection of neurotransmitters such as dopamine and its precursor levodopa from functional deactivation by methylation.
[0034] In yet another aspect, the sacrificial demethylation function of FDSP-EGCG acts to protect functional regions of p53 protein from methylation so that p53 can continue to perform repair work around DNA, as well as at tumor and cancer cells. [0035] In another aspect, the FDSP-EGCG composition provides a desulfurization property by sacrificial oxidation of a pendant phosphonate group. Regions of excess sulfur arise from a local excess of glutathione, leading to hydrophobic sulfur-protein bonds associ ated with the waxy region that separates tumor cells from the native immune system. The extraction of sulfur from cross-linked and mis-folded waxy protein agglomerates by FDSP-EGCG leads to a unique mode of tumor and cancer cell penetration to better allow the natural immune response access to these types of cells.
[0036] In a related aspect, the desulfurization function acts to free entrapped p53 protein which may become folded into and crosslinked with protein tangles associated with excessive glutathione in the regions around tumor and cancer cells.
[0037] In another aspect, FDSP-EGCG forms a complex with tp53 (p53) protein to avoid ubiquitin signaling tags that may otherwise reduce the effective lifetime of p53 DNA repair protein. FDSP-EGCG bonds to the N-terminus of the p53 (also known as tp53) to prevent premature catabolism of this endogenous DNA repair protein. Complexing FDSP-EGCG with the N-terminus of p53 is designed to block the well-known chemical association of p53 with double minute 2 homolog (DM2) also known as E3 ubiquitin-protein ligands. This prevents bonding of MDM2 to the transactivation domains of p53 at its tyrosine and tryptophan amino acid functional group residues. Especially, the aromatic region of FDSP-EGCG is designed to generate highly effective aromatic pi bonds to the central docking location of the N-terminus at tryptophan residue number 91 to confer maximum p53 protein service lifetime.
[0038] In another aspect, the nanoparticle ensemble amplifies the well-known bacteriostatic effect of EGCG by the bond to C60, especially for those bacteria that are known as “super bugs” because they have evolved a resistance to prescribed antibiotics.
[0039] In a related aspect, certain bacteria commonly live on the skirt of many people without causing harm. However, these bacteria can cause skin infections or buccal infections if they enter the body through cuts, open wounds, or other breaks in the skin. A clear alternative to prescribed antibiotics for mouth, skin, or gastric infections by pathological strains of antibiotic resistant bacteria is provided. Non-limiting examples of the types of bacteria that can be treated include methicillin-resistant Staphylococcus aureus (MRSA), group ‘A’ Streptococcus (GAS) or "strep" leading to ‘strep throat’, and Impetigo especially as it is most commonly found on the face as ruptured blisters that form a flat, thick, honey-colored (yellowish-brown) crust. [0040] In another aspect, delivery methods are provided. In one aspect of delivery, a nano- aerosolized composition carries the FDSP-EGCG in a carrier fluid dispenser, and the composition in gasified and delivered to the nose, mouth, trachea, and airways of a patient or user.
[0041] In another aspect of delivery, the FDSP-EGCG is adsorbed onto the pore structure of a mineral such as zeolite for oral administration and timed release into the intestinal tract wherein a variation of the silicon to aluminum ratio of this mineral, or a variation in the porosity of diatomaceous earth mineral, or like negative charged mineral, provides both a charged surface and different pore sizes and therefore a timed-release function.
[0042] In yet another aspect of delivery, the FDSP-EGCG is formulated into a topical cream carrier for application to the skin and the buccal cavity regions.
[0043] In yet another aspect of delivery, the FDSP-EGCG is formulated into an oral solution with sweeteners, flavors, and preservatives suitable to formulate a beverage or to be used as an additive to existing beverages such as traditional tea or coffee.
[0044] These and other advantages of the present invention will be further understood and appreciated by those skilled in the art by reference to the following written specification, claims, and appended drawings.
[0045] Some embodiments are described in detail with reference to the related drawings.
Additional embodiments, features, and/or advantages will become apparent from the ensuing description or may be learned by practicing the invention. In the illustrations, which are not drawn to scale, like numerals refer to like features throughout the description. The following description is not to be taken in a limiting sense but is made merely for describing the general principles of the invention.
BRIEF DESCRIPTION OF THE DRAWINGS [0046] Preferred embodiments of the invention will now be described, by way of example, with reference to the accompanying drawings, in which:
[0047] FIG. 1 is an illustration of the molecular structures of two exemplary polyhydroxyl phenyl containing molecules, EGCG and tannic acid.
[0048] FIG. 2 is an illustration of the molecular structures of alternative flavan-3-ols, being additional exemplary catechin gallate raw materials suitable as substitutes for EGCG.
[0049] FIG. 3 is an illustration of the molecular structures of commercially available fullerene disodium phosphonates (FDSP). [0050] FIG. 4 is an illustration of one pentagonal reacted portion of the molecular structure of fullerene disodium phosphonate (FDSP).
[0051] FIG. 5 is an illustration of the molecular structures for epigallocatechin gallate
(EGCG) reaction with buckminsterfullerene (C60).
[0052] FIG. 6 is an illustration of the molecular structures for epigallocatechin gallate
(EGCG) reaction with fullerene disodium phosphonate (FDSP).
[0053] FIG. 7 is an illustration of a desulfurization reaction leading to tumor penetration and complex formation with p53 protein.
[0054] FIG. 8 is an illustration of C60-EGCG or FDSP-EGCG packed into the pores of substrates for timed release, such as Transcarpathian zeolite (clinoptilolite) or diatomaeeous earth. [0055] FIG. 9 is an illustration of the method of synthesis of FDSP-EGCG formulated with solvents suitable for nano-aerosol administration.
[0056] FIG. 10 is an illustration of the method of synthesis of C60-EGCG and FDSP-EGCG formulated with flavors and preservatives suitable for water-based and solid based oral administrations.
[0057] FIG. 11 is an illustration of the method of creating FDSP-EGCG formulated with perfumes and thickeners suitable for topical skin administrations.
[0058] FIG. 12 is an illustration of personal administration of aspirated nano-aerosol containing the FDSP-EGCG nanoparticles.
[0059] FIG. 13 is an illustration of personal topical skin administration of FDSP-EGCG.
[0060] FIG. 14 is an illustration of experimental FTIR data for EGCG.
[0061] FIG. 15 is an illustration of experimental FTIR data for C60-EGCG.
[0062] FIG. 16 is an illustration of experimental FTIR data for C60-tannic acid.
[0063] FIG. 17 is an illustration of experimental FTIR data for FDSP.
[0064] FIG. 18 is an illustration of experimental FTIR data for FDSP-EGCG.
[0065] FIG. 19 is an illustration of experimental FTIR data for zeolite.
[0066] FIG. 20 is an illustration of experimental negative mode mass spectrograph data for buckminsterfullerene (C60).
[0067] FIG. 21 is an illustration of experimental negative mode mass spectrograph data for fullerene penta-di sodium phosphonate (FDSP).
[0068] FIG. 22 is an illustration of experimental negative mode mass spectrograph data for
C60-EGCG. [0069] FIG. 23 is an illustration of experimental negative mode mass spectrograph data for
FDSP-EGCG.
[0070] Some embodiments are described in detail with reference to the related drawings.
Additional embodiments, features, and/or advantages will become apparent from the ensuing description or may be learned by practicing the invention. In the illustrations, which are not drawn to scale, like numerals refer to like features throughout the description. The following description is not to be taken in a limiting sense but is made merely for describing the general principles of the invention.
DETAILED DESCRIPTION OF THE INVENTION [0071] The following detailed description, taken in conjunction with the accompanying drawings, is merely exemplary in nature and is not intended to limit the described embodiments or the application and uses of the described embodiments. Any implementation described herein as “exemplary” or “illustrative” is not necessarily to be construed as preferred or advantageous over other implementations.
[0072] Furthermore, there is no intention to be bound by any expressed or implied theory presented in the preceding technical field, background, brief summary or the following detailed description. It is also understood that the specific devices, systems, methods, and processes illustrated in the attached drawings, and described in the following specification, are simply exemplary embodiments of the inventive concepts defined in the appended claims that there may be variations to the drawings, steps, methods, or processes, depicted therein without departing from the spirit of the invention. All these variations are within the scope of the present invention. Hence, specific structural and functional details disclosed in relation to the exemplary embodiments described herein are not to be interpreted as limiting, but merely as a representative basis for teaching one skilled in the art to variously employ the present embodiments in virtually any appropriate form, and it will be apparent to those skilled in the art that the present invention may be practiced without these specific details.
[0073] Various terms used in the following detailed description are provided and included for giving a perspective understanding of the function, operation, and use of the present invention, and such terms are not intended to limit the embodiments, scope, claims, or use of the present invention.
[0074] FIG. 1 illustrates two of the most efficacious anti-cancer molecular structures 100 used in the present invention. The molecular structure of epigallocatechin gallate (EGCG) 110 is the most thermally stable and therefore the best catechin used as a raw material to make the derivatives and formulations for the present invention, having chemical formula C22H18G3 1. The bracketed region 120 of EGCG 110 is more generally known as the chromane or benzo dihydropyran functional group and is a common feature of the acceptable catechin polyphenols in this composition.
[0075] An acceptable replacement for EGCG 110 is the polygalloyl quinic acid esters with the number of galloyl moieties per molecule ranging from 2 up to 12, is represented by the tannin molecular structure 130 for tannic acid, also known as penta-m-digalloyl-glucose. Tannic acid is reproducibly about equal in anti-proliferative and anti -bacterial function to the efficacy of EGCG, which points to both EGCG 110 and tannic acid 130 as ideal candidates for development as functional groups to enhance their anti-cancer properties because of each expresses di -hydroxy phenyl or tri-hydroxy phenyl (more generally, polygalloyl) functionality in their outer molecular structures. Substances 110, 130 are used to help create, process, or deliver parts of the composition or their metabolites according to these teachings.
[0076] FIG. 2 is an illustration of the molecular structures of flavan-3-ols being exemplary catechins 200 that are acceptable substitutes for EGCG and may be independently selected from the flavan-3-ol group also known as catechins, or physiological metabolites thereof, to be reacted to produce the composition of the present invention. These benzo-hydropyran moieties provide at least one of a tri-hydroxyphenyl group or a di -hydroxy phenyl group of the composition. In some cases, it may be economically desirable to replace the EGCG catechin with an alternative thermally less stable benzo-hydropyran, such as the catechin having formula C15H14O6 (EC) 210, or the catechin having formula C15H14O7 (EGC) 220, or the catechin having formula C22H18G1 0 (ECG) 230, or catechin formula C15H14O6 (Robinetinidol) 240. Of these alternative selections, the three molecules 220, 230, 240 are experimentally well known to be more reactive polyphenols and can be used to increase the potency of the composition to make it more antimicrobial for some methods of use, such as for treating periodontal disease, than the di-hydroxylated catechin 210. In all cases, the catechin properties become greater and their chemical reactivities become significantly magnified then they are reacted as adducts to the fullerene disodium phosphonates (FDSP), according to the teachings of the present invention.
[0077] FIG. 3 illustrates two alternative side view's of the molecular structures for fullerene di sodium phosphonates (FDSP) 300. The commercial product FDSP is a core C.60 fullerene 310, 320 that is covalently bonded with five phosphonate groups 330, 340, 350, 360, 370, which can also be illustrated collectively as shown by the bracketed region 380. The FDSP used as a reactant to produce the FDSP catechin composition(s) of this invention can be obtained commercially. [0078] FIG. 4 illustrates a portion of a front view of a central pentagonal carbon region of the buckmiiisterfullerene (C60) molecular structure of FDSP 400. Five functional groups of di sodium phosphors ates 410, 420, 430, 440, 450 are covalently bonded proximal to a strained pentagonal region of the C60 molecular cage of FDSP. This substance is commercially available and is used as a reactive reagent to create the poly galloyl catechin derivatives of the present invention.
[0079] FIG. 5 illustrates the molecular structures for the epigallocatechin gallate (EGCG) reaction with buckminslerfullerene (C60) 500. The exemplary catechin, epigallocatechin gallate (EGCG) 510 is combined to react with buckminsterfullerene (C60) 520 in a reactive shear mill under conditions of shear mixing and pressure. A shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 and simultaneously shift the density of states of the electrons of the carbon cage into anisotropic electrostatic distributions. These electrostatic charges then achieve a metastable state when abutted proximal to simultaneously induced opposing electrostatic charges with at least one polygalloyl catechin, EGCG. This forms the product composition of C60-EGCG provided with pi-carbonyl, pi-cation, and hydrogen bonding where this reaction proceeds in the direction of the large black arrow. At least one aromatic region of the EGCG molecular structure 530 may form a pi-carbonyl bond 540 with the C60 group 550 to significantly stabilize the resulting molecular ensemble. Any carbonyl functional group of EGCG can create a pi-carbonyl bond 560 with an aromatic region of the C60 to further stabilize the C60-EGCG molecular structure. It is to be understood that any combination of the catechin gailates specified herein are acceptable substitutions for the EGCG molecules in the reaction with C60, according to the teachings of the present invention.
[0080] FIG. 6 illustrates a chemical reaction to form a C60-EGCG 600. The exemplary catechin, epigallocatechin gallate (EGCG) 610 is combined to react with fullerene disodium phosphonate (FDSP) 620 in a reactive shear mill under conditions of shear mixing and pressure. A shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 of the FDSP and simultaneously shift the density of states of the electrons of the carbon cage into anisotropic electrostatic distributions. These electrostatic charges then achieve a metastable state when abutted proximal to simultaneously induced opposing electrostatic charges with at least one catechin gallate, EGCG. This forms the product composition of FDSP-EGCG provided with pi-carbonyl, pi-cation, and hydrogen bonding where this reaction proceeds in the direction of the large black arrow. FDSP-EGCG contains a multiplicity of diphosphonate functional groups that may disassociate at least one migrating sodium ion 630 to leave at least one corresponding pendant negative charged anionic phosphonate 640 capable of forming a hydrogen bond 650, illustrated as a dotted line, with any hydroxyl group of the EGCG structural region. The cationic sodium is then able to form a pi-cation bond 660 with any aromatic region of the core C60 functional group 670 of EGCG-FDSP. At least one aromatic region of the EGCG molecular structure 680 may form a pi-carbonyl bond 690 with the C60 functional group to significantly stabilize the resulting molecular ensemble. Any carbonyl functional group of EGCG can create a pi-carbonyl bond 695 with an aromatic region of the C60 functional group to further stabilize the EGCG-FDSP molecular structure. It is to be understood that any combination of the catechin gallates specified herein are acceptable substitutions for the EGCG molecules in the reaction with FDSP, according to the teachings of the present invention.
[0081] FIG. 7 illustrates a desulfurization reaction of FDSP-EGCG 700, leading to tumor penetration. The core tetramer of the endogenous DNA repair protein p53 is represented herein as a schematic geometric form 710. The complete structure of the p53 protein with all amino acid residues is available at the protein data bank under rcsb.org/stmcture/2XWR. The context of a p53 core tetramer bound to an arbitrary region of DNA being repaired is publicly available under reference code 3EX.J under rcsb.org/structure/3EXJ. A protective pi-bond formation to tp53 (p53) protein 720 shown by a dashed line, protects p53 from obtaining a ubiquitin signaling tag that may otherwise reduce the effective lifetime of p53, which functions to repair DNA in the cell. The EGCG is provided with aromatic pi to carbonyl bonds 730, and with aromatic pi to aromatic pi stacking bonds 740, 750 to the C60 functional group as shown by dashed lines in this molecular structure.
[0082] The presence of phosphonate groups of oxidation state three is provided in the molecular structure of FDSP-EGCG to penetrate the sulfur-rich hydrophobic region of tumors and metastasized cancer cells, as well as to desulfurize fungal proteases associated with respiratory pathology such as valley fever, or to desulfurize viral proteases. The phosphonate sulfurization reaction proceeds by extraction of sulfur (S) as indicated by the black arrow 750, where the source of extracted sulfur can be a local excess of glutathione and sulfur-protein bonds associated with the waxy region that separates tumor cells from the native immune system carried by aqueous phase physiological plasma such as blood in the circulatory system. One of the phosphonate groups of FDSP-EGCG is sulfurized by the acquisition of a sulfur atom 760. The sulfurization results in a sulfurized phosphonate having phosphorus of oxidation state 5. Sulfurization demonstrates the superiority of FDSP-EGCG over C60-EGCG in penetrating the regions where p53 was rendered inactive by protein misfolding and entrapment by sulfur bonded protein regions.
[0083] A primary intended result of freeing p53 from protein entrapment by sulfur adducts, is to further extend the service life and anti-tumor function of p53 by the facile hydrogen bond association of the acidic hydroxyl groups of the EDSP-EGCG with the disordered and splayed hydroxyl groups of the N -terminal region of p53 proteins. This is achieved by complexing FDSP- EGCG with the N- terminus of p53 to block the chemical association of DM2 to the transactivation domains of p53, being localized to a few tyrosine and tryptophan amino acid functional group residues. The DM2 bound to the transactivation domain of p53 (not shown) is publicly available at rcsb.org/structure/lYCR. The aromatic region of FDSP-EGCG is complexed at the central docking location of the p53 N-terminus (medically known to be at or about the amino acid residue number 91 for tryptophan) by means of aromatic pi-pi bonding to the C60 functional group 780 thereby significantly stabilizing this complex and improving the DNA repair function of p53 beyond that expected for the unreinforced p53 protein in its natural state without a FDSP-EGCG complex. The remaining four disodium phosphonate groups 790 may continue to act as desulfurization agents, as these can provide additional subsequent desulfurization reactions, thereby enabling p53 protein complexed with FDSP-EGCG to penetrate even more deeply into the waxy sulfurized coatings around tumor and cancer cells, according to the teachings of the present invention.
[0084] FIG. 8 illustrates the porous substrate zeolite or diatom aceous earth impregnated with C60-EGCG or FDSP-EGCG 800. Transcarpathian zeolite (clinoptilolite) 810 is a type of mineral provided with a highly negatively charged network structure achieving a system of reproducible and well-defined pores and channels. Clinoptilolite zeolite Is well known to adsorb nitrogen containing compounds including ammonia, amino acids, and other positive charged molecules. Similarly, Clinoptilolite zeolite is optionally used herein to adsorb thiamine (vitamin Bl) as positive counter-ion and hydrogen bonding adduct. The thiamine adducts can be used to stabilize the impregnation with the composition of FDSP-EGCG in the form of a multiplicity of clusters 820, 830, 840, 850, 860, and 870 having cluster sizes sufficiently small to fit within the pore regions, being greater than 100 nanometers and less than about 5 microns in size. It is also known that at pH greater than 7, as well as under saline or physiological ionic salt conditions, clinoptilolite zeolite displaces and expresses the positively charged nitrogen compounds and counterions stored within the pores. The salt and pH moderated regenerant property of clinoptilolite towards reversible expression and release of positively charged nitrogen compounds has led to the widespread economic commercial adoption of clinoptilolite Transcarpathian zeolite as a dietary supplement.
[0085] Diatomaceous earth is a silicate bearing mineral composed of a multiplicity of silicon dioxide skeletons of diatoms having a multitude of shapes 880, 885 and being from 30 to about 200 nanometers in size. The negatively charged diatomaceous silicates can adsorb thiamine (vitamin Bl) as a positive counter-ion and hydrogen bonding adduct; these adducts stabilize the impregnation with the composition of FDSP-EGCG in the form of a multiplicity of clusters 890, 895. At pH greater than 7, as w'ell as under saline or physiological ionic salt conditions, diatomaceous earth slowly diffusion releases and expresses FDSP-EGCG and thiamine counterions stored within the pores and the spaces between the silicate structures of the diatoms to achieve a timed-release of the FDSP-EGCG composition into the digestive tract.
[0086] The counter ion-exchange property of Transcarpathian zeolite (clinoptilolite) or diatomaceous earth or other solid pharmaceutical grade minerals may be used as adjuvant delivery or timed-release delivery in any combination whatsoever, to perform timed digestive release of the composition of the present invention as one method of oral delivery of the composition of the present invention, according to these teachings.
[0087] FIG. 9 is a flowchart representation of an exemplary scalable synthesis method S900 of nano-aerosol FDSP-EGCG formulated for nano-aerosol administration. In step S910 To one mole of FDSP, add 5 moles of a catechin; EGCG is the preferred catechin. In step S920 the prepared dry powder mixture is reaction shear milled at about 55 °C to achieve the desired FDSP- EGCG product whereby a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 of the FDSP and simultaneously shift the density of states of the electrons of this cage molecule into anisotropic electrostatic distributions that achieve a metastable state when abutted to simultaneously induced opposing electrostatic charges with least one abutting proximal EGCG polyphenol. In step S930, the desired concentration of FDSP-EGCG is created by dissolving a weighed amount of the dry powder into a 70% glycerol and 30% polypropylene glycol solvent mixture by volume, in step S940, a metered amount of the nano aerosol fluid from step S930 is generated by a commercially available electronic dispensing device suitable for client inhalant aspiration by means of a heated airflow between about 255 °C and 300 °C to create the nano-aerosol, according to the teachings of the present invention.
[0088] FIG. 10 is a flowchart representation of an exemplary scalable method S1000 for synthesis of oral administered FDSP-poly galloyl. In step SI 010 about 1 mole of commercially available FDSP is combined with nominal 5 moles of a catechin, EGCG or tannic acid. In step S1020 the combined mixtures are milled at about 1000/sec shear rate and about 55 °C to achieve the desired FDSP-EGCG reaction product. In this process, a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the C60 of the FDSP and simultaneously shift the density of states of the electrons of this cage molecule into anisotropic electrostatic distributions that achieve a metastable state when abutted to simultaneously induced opposing electrostatic charges with least one abutting proximal EGCG polyphenol. In step 1030, the FDSP-EGCG from step 1020 is mixed into food grade slow-release solid carrier material such as a Transcarpathian zeolite (clinoptilolite), diatomaceous earth, or like porous solid phase. This operation is substantially enabled by the introduction of a vitamin B1 additive to assist with creating a positive counter-ion charge coupling with both the negative charges in the porous solid phase and the negative charges in the FDSP-EGCG. This process can be performed in a standard industrial kneading device such as food processing mixers that are typically used for making bread. In step S1040, the desired concentration of FDSP-EGCG is created by dissolving a weighed amount of the dry powder mixture with the porous scaffold component into a mold for pressing into an oral tablet. Alternatively, a weighed dosage of this power mixture is filled into capsules to be administered for oral administration of the time-release formulation. This serving size or dosage may then be dispersed into any amount of water if desired, prior to oral administration, to ease consumption. It is understood that such a water dispersion of the time-release formulation is unstable and subject to settling on standing for periods of greater than a few hours. When desired, this formulation may be dispensed into aqueous media for later distribution at any time for later oral administration, with the provision of optional viscosity modifiers that can be added to this mixture to stabilize the insoluble mineral components from settling therein as a minor variation to this method. This enhances the long-term esthetic appeal of the solid dispersed into an aqueous medium, while simultaneously maintaining the time-release feature of the porous solid insoluble carriers, according to these teachings.
[0089] FIG. 11 is a flowchart representation of an exemplary scalable method S 1100 for producing and applying a topical skin or buccal administered FDSP-catechins. In step S1110 To one mole of FDSS , add 5 moles of a catechin as in the preferred EGCG. It is to be understood that any of the catechins EC (epicatechin), epicatechin gallate (EGG). or epigallocatechin (EGC) can substitute for epigallocatechin gallate (EGCG). It should be noted that the use of poly-galloyl tannic acid for beauty products should be avoided because it may stain the skin, but when the topical formula is to treat skin cancer or esophageal cancer, the catechins should be avoided as tannic acid forms a substantially greater surface bond to skin and mouth cancer cells. In step S1120 the mixture of step S1 110 is reaction shear milled at about 55°C to achieve the desired FDSP reaction product. In this process, a shear pressure of about 20 grams per square micron is sufficient to create a slightly geometric oblate spheroid of the 6C0 of the FDSP and simultaneously shift the density of states of the electrons of this cage molecule into anisotropic electrostatic distributions that achieve a metastable state when abutted to simultaneously induced opposing electrostatic charges with least one abutting proximal catechin polyphenol such as EGCG. In step SI 130 the product of Step 1120 is dissolved into water. For topical skin formulations, about 1% to 2% of the product is added to hyaluronic acid, along with about 4% perfume, a desired amount of methacrylic acid for viscosity enhancement, and 1% preservative. However, for buccal solutions, a commercially available gelatin and desired flavors can be used along with 1% sodium sorbate as a food preservative. In step SI 140, the pH of the acidic FDSP-EGCG composition is adjusted to prevent mold or bacterial growth with an acceptable range of 5 to 6.7 and a nominal value of 6.5 by neutralization with sodium hydroxide (NaOH) with adequate mixing to ensure a uniform cream or lotion. In step S1150, this material composition is transferred into beauty and cosmetic cream jars or tubes having a sufficiently hermetic seal to able to retain the volatile aromas or flavorings. In step 1160, the face is washed to remove natural skin residues prior to applying the topical formulation, such as before bedtime. The buccal formulation can be applied, for example, after brushing the teeth.
[0090] FIG. 12 illustrates a method SI 200 for the personal administration of aspirated nano-aerosol delivery of fullerene penta-di sodium phosphonate epigallocatechin gallate (FDSP- EGCG). The nano-aerosol generating device 1210 filled with FDSP-EGCG dispensing solution is provided for dispersing and nebulizing the inhalant gas including the nano-particles. The device 1210 may also be more commonly known as a nebulizer, or an electronic vaporizing device, or an electronic cigarette, or the functional part of a hookah to be shared among several users. In all cases these systems serve to carry the FDSP-EGCG in a carrier fluid dispenser 1210, and to transfer that composition in nebulized form along with an aerosolized solvent in a substantially gaseous dispersion to the nose, mouth, trachea, and airways of a patient or user 1220. One intended use of the FDSP-EGCG composition is to treat, delay or arrest the incidence of cancers wherein the nano-aerosol can expedite targeted delivery to the brain by avoiding a passage through the digestive system.
[0091] Some of the nano-aerosolized composition is exhaled and shown as particulate clusters 1230, 1240, 1250 within exhaled smoke puffs 1260 and 1270 emitted on exhalation as indicated by the direction of thin line arrows radiating away from the nose of the subject 1220. Delivery of the C60-FDSP-EGCG nano-aerosol composition from dispenser 1210 provides antioxidant properties to the mucus airway tissues wherein destruction of free radicals and oxidants associated with motor neuron disease and Parkinson’s disease are part of the treatment and alpha-synuclein plaque mitigation is provided. Systems that may be used for the method of dispersion of the FDSP-EGCG represented by dispenser 1210, include, without limitation, any of the electronic cigarette devices produced internationally and listed in Appendix 4.1, '‘Major E- cigarette Manufacturers” of the “2016 Surgeon General's Report: E-Cigarette Use Among Youth and Young Adults” published by the Center for Disease Control and Prevention (CDC), Office of Smoking and Health (OSH) freely available at the CDC.GOV website, or any combination of piezoelectric, resist] vely heated, or inductively heated vaporized fluid delivery methods that can be utilized to deliver the composition of the present invention, especially when approved as a medical drug delivery device. Each embodied variation of such methods without limit are intended to aspirate aerosols as the method of therapeutic substance delivery of the composition of the present invention directed into the nasal cavities, mouth, tracheal breathing orifice, or intubated trachea of a patient. The supply direction of nebulized feed of FDSP-EGCG on inhalation and exhalation are delivered into the airways and lungs of the intended patient by the flow' of supplied air as indicated by the direction of upward and downward facing large white arrows 1280, when used according to these teachings.
[0092] FIG. 13 illustrates personal topical skin administration and buccal administration of fullerene penta-(di sodium phosphonate) epigallocatechin gallate (FDSP-EGCG) composition 1300. A semi-liquid slurry dispersion, cream, ointment, or lotion can he used to contain and transfer the administered formula, as a somewhat different formulation is required depending on whether the application is for topical use on the skin or to coat tooth surfaces in the oral or buccal cavity, which is located inside the mouth 1310. The skin care formula can he applied by the user 1320 in regions such as the face 1330, 1340. Application of the skin care formulation can be by means of circular rubbing motions as indicated by the direction of arrows 1350, 1360. The skin- care formulation then confers topical antimicrobial properties such as for MRS A and other antibiotic resistant skin infections, anti-aging and skin brightening functions, and to promote resistance to the onset of skin cancers. In the case of the buccal administration to the oral cavity, which is located inside the mouth 1310, the oral mucosal antibacterial functions of the nanoparticle ensemble are to promote anti-gingivitis properties, such as to treat periodontal disease, especially for those bacteria that are known as “super bugs” because they have evolved a resistance to prescribed antibiotics, as well as to promote anti-esophageal cancer treatment properties. Non- limiting examples of the type of bacteria that can be treated include methicillin-resistant Staphylococcus aureus (MRSA), group ‘A’ Streptococcus (GAS) or "strep” leading to ‘strep throat’, and Impetigo especially as commonly found on the face in the form of ruptured blisters that form a flat, thick, honey-colored (yellowish-brown) crust. The formulations are to be synthesized and administered according to the teachings of the present invention.
[0093] FIG. 14 illustrates experimental FTIR data for EGCG raw material The sample was prepared by the method of mixing, crushing, and consolidating under 7 metric tons of pressure, about 0.001 grams of analyte with 1 gram of a diluent solid material that is substantially transparent to infrared light, this diluent being anhydrous potassium bromide (KBr), which then flows under pressure to form a translucent pellet of about 0.4 mm thickness. Spectral background subtraction in air using a control pellet of the same mass and thickness having pure KBr was used to obtain a baseline instrument infrared spectral transmission response. This method is generally referred to as the ‘KBr pellet’ sample preparation method, and it is used hereinafter throughout for each FTIR experimental data collection and spectral analysis. The Fourier transform infrared spectrophotometer used herein to obtain FTIR spectra throughout, Is a model RF6000 FTIR instrument manufactured by Shimadzu of Japan.
[0094] The sample of EGCG analyte prepared by KBr pellet obtains a broad characteristic absorbance from 3100 cm-1 to 3600 cm-1 arising from hydroxyl (OH) functional groups bonded to each aromatic ring. Absorbances at 1609 cm-1, and 1646 cm-1 arise from the carbonyl group (C=0) that links between the trihydroxybenzoate group and the chromane (benzo dihydropyran) ring. The absorbance at 1450 cm-1 arises from the C-H group present in the Chromane ring. Absorbances at 1150 cm-1 and 1091 cm-1 are attributed to the hydroxyl (OH) group, and the peak at 817 cm-1 is attributed to carbon-hydrogen (CH) stretch pendant from the aromatic ring. Comparison of the illustrated experimental FTIR data for EGCG 1800 indicates similarity to the FTIR absorbances reported for EGCG that are generally available from the scientific literature for confirmation of this reactant material when used according to the teachings of the present invention,
[0095] FIG. 15 illustrates experimental FTIR data for C60-EGCG. A very strong and sharp
C60 fullerene aromatic carbon-carbon (C-C) stretching band appears at 576 cm4 and, and a less intense but also sharp carbon-carbon absorbance appears at 526 cm-1. Constrained carbon- hydrogen stretching bands appear at 2921 cm-1 and 2851 cm-1 attributed to the likely interaction of the EGCG ring structures with the fullerene ring structure through aromatic pi bonding. The pure EGCG carbonyl absorbance at 1646 cm-1 shown in FIG. 14 is now seen to be decreased in intensity and shifted to 1684 cm-1; however, the pure EGCG carbonyl absorbance at 1609 cm-1 shown in FIG. 14 is verifiably identical in intensity and remains at 1609 cm-3. These carbonyl characteristics are attributed to a shifted stretching angle of the proximal chromane group through the oxygen bridge next to the carbonyl group and provide evidence for an altered geometry of the EGCG functional group as it wraps around the fullerene group through aromatic pi interactions. This design feature shows that the geometry of the ECGC functional moiety has been altered but the acidity of the hydroxyl groups has been preserved in a manner that favors the chemical interaction of C60-EGCG as a demethylating agent or a sacrificial methyl group sponge. These changes in infrared absorbances are attributed to altered or spatially confined geometry impacting bond mobility in bending and stretching modes associated with the formation of pi-pi bonds according to the teachings of the present invention.
[0096] FIG. 16 illustrates experimental FTIR data for C60-tannic acid. Characteristic sharp
C60 fullerene aromatic carbon-carbon stretching bands appear at 526 cm-1 and 576 cm-1. Two carbonyl (C=0) bands having different molecular environments appear at 1609 cm-1 and at 1706 cm-1. The central glucose molecule makes strong carbon-oxygen vibrational contributions at 1197 cm-1 and 1318 cm-1. The broad absorbance region from 3680 cm-1 to about 2870 cm-1 is attributed to the hydroxyl functional groups of the poly-galloyl structures in this molecule.
[0097] FIG. 17 illustrates experimental FTIR data for fullerene disodium phosphonate
(FDSP). A characteristic and very strong and sharp C60 fullerene aromatic carbon-carbon stretching band appears 526 cm-1. The strong and broad absorbance peak ranging from 3250 cm-1 to 3650 cm-1 is attributed to hydroxyl functional groups pendant from the phosphonate adducts which have not been completely neutralized by sodium ions. The absorbance at 1581 cm-1 is attributed to phosphorous-oxygen stretching and are associated with fullerene phosphonates characteristic for purposes of identification when this reactant is used according to the teachings of the present invention,
[0098] FIG. 18 illustrates experimental FTIR data for fullerene disodium phosphonate - epigallocatechin gallate (FDSP-EGCG). A significant new absorbance at 1073 cm-1 is observed that corresponds with the disappearance of previously observed absorbances at 1150 cm-1 and 1091 cm-1 attributed to the hydroxyl (OH) groups of EGCG as described for FIG. 14. This effect is attributed to the change of the EGCG hydroxyl group stretch from being dissimilar in two regions with respect to planar phenolic symmetry to a uniformly constrained hydroxyl group stretch that is geometrically dominated by the proximal phosphonate groups at their distal ends, and the proximal fullerene with pi-pi bonds appending to each aromatic phenyl group of the EGCG, wherein these combined effects indicate a strongly held and partially wrapped configuration. This design feature shows that both the geometry as well as the acidity of the EGCG functional moiety, according to these teachings.
[0099] FIG. 19 illustrates experimental FTIR data for the natural Transcarpathian zeolite
(clinoptilolite). There are over 40 well known natural zeolites and about 160 synthetic zeolites, where the synthetic zeolites have fine control over pore size as compared with natural zeolites due to their purity and control over the silicon to aluminum ratio; herein it is desirable to have the natural zeolite provided with a wide range of pore sizes to suit the timed-release application. It is to be understood that the use of synthetic zeolites, especially a blend of synthetic zeolites having different pore sizes, are an equivalent and acceptable substitution. The broad absorbance peak observed at 3441 cm- 1 is attributed to the existence of the adsorbed water hydroxyl group stretching vibrations (OH). The absorbances at 2918 cm-1 and 2850 cm- 1 arise from trace carbon hydrogen stretching of organic materials adsorbed onto the structure of the natural zeolite. The absorbances at 1634 cm-1 and 1574 cm-1 are attributed to two different types of steric environments associated with hydroxyl (OH) bending vibrations. The absorbance peak at 1455 cm-1 is attributed to adsorbed amine contributing to a signal of nitrogen-hydrogen bending (-NH) which correlates with the absorbance at 3626 cm-1 for nitrogen-hydrogen (NH) stretching vibrations. The very intense and broad beak at 1031 cm-1 has a characteristic shoulder absorbance at 1197 cm-'1 which collectively characterize the primary absorbance patterns of zeolite arising from its aluminum-oxygen (AIO) and silicon-oxygen (SiO) bending vibrations, where the position of this band depends on the aluminum to silicon ratio and determines the number of the A1 atoms per formula unit. The asymmetric stretching due to the internal vibrations of the zeolite silicon-oxygen (SiO) framework tetrahedra occurred at 790 cm-3. The symmetric stretching due to the internal vibrations of silicon- oxygen (SiO) framework tetrahedra is attributed to the absorbance peak at 719 cm-1. The symmetric stretching due to the internal vibrations of negatively charged silicon-oxygen (SiG(-)) framework is attributed to the absorbance peak at 586 cm-1. The strong absorbance at 464 cm-1 is attributed to the bending of the zeolite framework tetrahedra. The experimental FTIR data for this natural Transcarpathian zeolite (clinoptilolite) indicates significant chemical similarity to the FTIR absorbances reported for zeolite that is generally available from the scientific literature and may be used for confirmation of the identity of zeolite absorbent material used to make a timed- release nanoparticle filled catechin formulation for the method of inclusion and carrying of those materials, according to these teachings.
[0100] FIG. 20 illustrates experimental negative mode MALDI-TOF mass spectrograph data for Buc kminsterfull eerene (C60). This sample, as well as each of the subsequent MALDI- TOF experimental test results hereinafter, was introduced for test by laser vaporization into a Voyager Mass Spectrograph from Applied Biosystems (Foster City, California, USA). Negative mode bombardment was by fast moving electrons at about 70 eV energy. This resulted in molecular fragmentation and electron removal from the highest molecular orbital energy as molecular ions were formed. The ratio of mass to charge (rn/z) is used to determine the molecular ion fragments to help determine the pieces of the original molecule in this assay. The mass peak at 720 m/z corresponds to the molecular ion of fullerene C60. The overall experimental test results characterize the molecular ion breakdown product of buckmlnsterfullerene (C60), where C60 may be used to further synthesize the composition of the present invention.
[0101] FIG. 21 illustrates experimental negative mode MALDI-TOF mass spectrograph data for FDSP. Negative mode bombardment was by fast moving electrons at about 70 eV energy. The mass peak at 720 m/z corresponds to the molecular ion of fullerene C60 functional group. The large number of sharp peaks with a cluster maximum at about 1967 m/z are attributed to the spallation products of partially ablated disodium phosphonate functional groups. The overall experimental test results characterize the molecular ion breakdown products of FDSP, where FDSP may be purchased commercially or can be synthesized as explained herein and then is to be used to further synthesize the composition of the present invention.
[0102] FIG. 22 illustrates experimental negative mode MALDI-TOF mass spectrograph data for C60-EGCG. The appearance of a multiplicity of spikes having separation of mass to charge ratio of 24 are attributed to the loss of dicarbide ions (C-C) associated with the presence of pi-pi aromatic bonds. The primary peak at about 726 mass to charge ratio is attributed to the fullerene functional group of 720 m/z having 6 adducts of hydrogen, and the cluster of peaks at about 1443 m/z are attributed to the presence of non-covalent pi-pi intercalation of EGCG, some of which may be shared between dimeric fullerene functional groups. The minor trace of peaks above this mass may indicate some traces of multimeric fullerene chains with signals below a threshold that is useful for interpretation and analysis. The characteristic mass spallation patterns are consistent with and representative of the formation of C60-EGCG for this component of the composition according to the teachings of the present invention;
[0103] FIG. 23 illustrates experimental negative mode MALDI-TOF mass spectrograph data for FDSP-EGCG. The mass peak at 723 m/z corresponds to the molecular ion fragment of fullerene C60 adduct with three residual hydrogen atoms, a unique feature of the main molecular spallation ion of this product. The complicated sharp rider peaks are attributed to the mass fragments of phosphonates as these disassemble from the base fullerene group. The peak at 1419 m/z is attributed to the presence of the non-covalent pi-pi intercalation of EGCG, some of which may be shared between dimeric fullerene functional groups, whereas the peaks centered at about 2042 m/z are attributed to the additional presence of covalently bonded disodium phosphonate groups which are also pendant from the fullerene functional group. The characteristic mass spallation patterns for the illustrated MALDI-TOF data are consistent with and representative of the formation of FDSP-EGCG for this component of the composition according to the teachings of the present invention.
[0104] As variations, combinations and modifications may be made in the construction and methods herein described and illustrated without departing from the scope of the invention, it is intended that all matter contained in the foregoing description or shown in the accompanying drawings shall be interpreted as illustrative rather than limiting. Thus, the breadth and scope of the present invention should not be limited by any of the above-described exemplary embodiments but defined in accordance with the foregoing claims appended hereto and their equivalents.

Claims

CLAIMS What is claimed is:
1. A nanoparticle composition comprising: buckminsterfullerene (C60) bonded to a poly digalloyl molecule.
2. The nanoparticle composition of claim 1 wherein a bond between the C60 and the poly digalloyl molecule is a pi bond.
3. The nanoparticle composition of claim 1 or 2 wherein the poly digalloyl molecule comprises a quinic acid ester having either di-hydroxyphenyl groups or tri-hydroxy phenyl groups.
4. The nanoparticle composition of claim 1 or 2 wherein the poly digalloyl molecule comprises epicatechin, epieatechin gallate, epigallocatechin, or epigallocatechin gallate.
5. The nanoparticle composition of claim 1 or 2 wherein the poly digalloyl molecule comprises penta-m-digalloy 1-glucose.
6. The nanopardcle composition of claim 1-4, or 5 wherein the C60 is further bonded to a disodium phosphonate functional group.
7. The nanoparticle composition of claim 6 wherein the C60 is bonded to the disodium phosphonate functional group and further bonded to four additional phosphonate functional groups.
8. The nanoparticle composition of claim 1-6 or 7 farther comprising a zeolite, wherein the C60 bonded to the poly digalloyl molecule is disposed within the zeolite.
9. The nanoparticle composition of claim 1-7 or 8 farther comprising diatomaceous earth, wherein the C60 bonded to the poly digalloyl molecule is disposed within porous diatom particles of the diatomaceous earth.
10. The nanoparticle composition of claim 1-8 or 9 further comprising a solvent, wherein the C60 bonded to the poly digalloyl molecule is disposed in the solvent.
11. The nanoparticle composition of claim 10 wherein the solvent comprises a mixture of 70% glycerol and 30% polypropylene glycol by volume.
12. A method of curing, treating, or prophylactically avoiding cancer, valley fever, or COPD in a subject, the method comprising the step of: administering to the subject an effective amount of a composition including a buckminsterfullerene (C60) bonded to a poly digalloyl molecule.
13. A method of curing, treating, or prophylactically avoiding an antibiotic resistant strain of bacteria in a subject, the method comprising the step of: administering to the subject an effective amount of a composition including a buckminsterfullerene (C60) bonded to a poly dig alloy 1 molecule.
14. The method of claim 12 or 13 wherein the composition includes a pharmaceutically acceptable carrier and the C60 bonded to the poly digalloyl molecule is disposed in the pharmaceutically acceptable carrier.
15. The method of claim 14 wherein the pharmaceutically acceptable carrier comprises a zeolite or diatomaceous earth.
16. The method of claim 14 wherein the composition disposed in the pharmaceutically acceptable carrier comprises a tablet, capsule, pill, powder, granule, or a liquid.
17. The method of claim 12-15 or 16 wherein administering the composition comprises administration by an intravenous, intramuscular, subcutaneous, intrathecal, intraperitoneal, topical, nasal, or oral route.
18. The method of claim 12 or 13 wherein administering the composition comprises administering an oral dosage including up to about 500 rng of the C60 bonded to the poly digalloyl molecule.
19. The method of claim 12 or 13 wherein administering the composition comprises administering an intramuscular, intravenous, or a subcutaneous dose of the C60 bonded to the catechin molecule in an amount of from about 0.1 mg/'Kg to about 5 mg/ Kg.
20. The method of claim 12 or 13 wherein administering the composition comprises administering a nano aerosol, a vapor, a powder, a dust, or an aerosolized inhalant.
21. The method of claim 12-19 or 20 wherein the poly digalloyl molecule comprises epicatechin, eplcatechin gallate, eplgallocateehin, or eplgallocateehin gallate.
22. The method of claim 12-20 or 21 wherein the C60 is further bonded to a disodium phosphonate functional group.
23. A method of making a nanoparticle, the method comprising: bonding a buckminsterfullerene (C60) to a poly galloyl molecule.
24. The method of claim 23 wherein the poly galloyl molecule comprises epicatechin, epicatechin gallate, eplgallocateehin, or eplgallocateehin gallate.
25. The method of claim 23 or 24 wherein the C60 is further bonded to a disodium phosphonate functional group.
26. The method of claim 23, 24, or 25 wherein bonding the disodium phosphonate functional group to the C60 is performed by reaction shear mixing.
27. The method of claim 23-25, or 26 wherein bonding the poly galloyl molecule to the C60 is performed by reaction shear mixing.
28. The method of claim 23-26, or 27 wherein bonding the poly galloyl molecule to the C60 is performed at no more than about 55 °C.
PCT/US2022/014454 2021-03-15 2022-01-28 Fullerene phosphonate poly di-galloyls and methods WO2022197376A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP22771889.7A EP4308532A1 (en) 2021-03-15 2022-01-28 Fullerene phosphonate poly di-galloyls and methods
CN202280032624.4A CN117295704A (en) 2021-03-15 2022-01-28 Fullerene phosphate polydigalloyl and method
US17/592,899 US20220288212A1 (en) 2021-03-15 2022-02-04 Fullerene phosphonate galloyls and methods

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202163161310P 2021-03-15 2021-03-15
US63/161,310 2021-03-15
PCT/US2022/012369 WO2022159332A1 (en) 2021-01-20 2022-01-13 Fullerenic ellagic luteolin and methods
USPCT/US2022/012369 2022-01-13

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/012369 Continuation-In-Part WO2022159332A1 (en) 2021-01-20 2022-01-13 Fullerenic ellagic luteolin and methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/592,899 Continuation-In-Part US20220288212A1 (en) 2021-03-15 2022-02-04 Fullerene phosphonate galloyls and methods

Publications (1)

Publication Number Publication Date
WO2022197376A1 true WO2022197376A1 (en) 2022-09-22

Family

ID=83320725

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/014454 WO2022197376A1 (en) 2021-03-15 2022-01-28 Fullerene phosphonate poly di-galloyls and methods

Country Status (1)

Country Link
WO (1) WO2022197376A1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020098180A1 (en) * 2000-03-24 2002-07-25 Huan-Yao Lei Fullerene pharmaceutical compositions for preventing or treating disorders
US20080004345A1 (en) * 2004-03-31 2008-01-03 Yasuhiko Tabata Novel Water-Soluble Fullerene, Process for Producing the Same and Active Oxygen Generator Containing the Fullerene
US20090162277A1 (en) * 2005-11-03 2009-06-25 Clemson University Lysophospholipids Solubilized Single-Walled Carbon Nanotubes
US20120189492A1 (en) * 2009-06-24 2012-07-26 William Marsh Rice University Fullerene compositions and methods for photochemical purification
WO2019184419A1 (en) * 2018-03-27 2019-10-03 厦门福慈生物科技有限公司 Use of fullerene compound, fullerene microcapsule powder and preparation method and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020098180A1 (en) * 2000-03-24 2002-07-25 Huan-Yao Lei Fullerene pharmaceutical compositions for preventing or treating disorders
US20080004345A1 (en) * 2004-03-31 2008-01-03 Yasuhiko Tabata Novel Water-Soluble Fullerene, Process for Producing the Same and Active Oxygen Generator Containing the Fullerene
US20090162277A1 (en) * 2005-11-03 2009-06-25 Clemson University Lysophospholipids Solubilized Single-Walled Carbon Nanotubes
US20120189492A1 (en) * 2009-06-24 2012-07-26 William Marsh Rice University Fullerene compositions and methods for photochemical purification
WO2019184419A1 (en) * 2018-03-27 2019-10-03 厦门福慈生物科技有限公司 Use of fullerene compound, fullerene microcapsule powder and preparation method and application thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SALONEN EMPPU, LIN SIJIE, REID MICHELLE L., ALLEGOOD MARCUS, WANG XI, RAO APPARAO M., VATTULAINEN ILPO, KE PU CHUN: "Real-Time Translocation of Fullerene Reveals Cell Contraction", SMALL, WILEY, vol. 4, no. 11, 1 November 2008 (2008-11-01), pages 1986 - 1992, XP055972332, ISSN: 1613-6810, DOI: 10.1002/smll.200701279 *
WANG ZHUANG, CHEN JINGWEN, SUN QIAN, PEIJNENBURG WILLIE J.G.M.: "C60-DOM interactions and effects on C60 apparent solubility: A molecular mechanics and density functional theory study", ENVIRONMENT INTERNATIONAL., PERGAMON PRESS., US, vol. 37, no. 6, 1 August 2011 (2011-08-01), US , pages 1078 - 1082, XP055972315, ISSN: 0160-4120, DOI: 10.1016/j.envint.2011.02.016 *

Similar Documents

Publication Publication Date Title
JP2016222689A (en) Compositions for treating pain
JP7003045B2 (en) Methods and pharmaceutical compositions for the treatment of neurodegeneration
Hu et al. Zinc oxide nanoparticles (ZnO NPs) combined with cisplatin and gemcitabine inhibits tumor activity of NSCLC cells
Jiang et al. Morphology-directed radiosensitization of MoSe2 nanoplatforms for promoting cervical cancer radiotherapy
KR101999421B1 (en) Caged platinum nanoclusters for anticancer chemotherapeutics
Wei et al. Epigallocatechin-3-gallate (EGCG) based metal-polyphenol nanoformulations alleviates chondrocytes inflammation by modulating synovial macrophages polarization
Cui et al. Folic acid-modified disulfiram/Zn-IRMOF3 nanoparticles for oral cancer therapy by inhibiting ALDH1A1+ cancer stem cells
WO2022197376A1 (en) Fullerene phosphonate poly di-galloyls and methods
EP4308532A1 (en) Fullerene phosphonate poly di-galloyls and methods
Wang et al. Engineered inhaled nanocatalytic therapy for ischemic cerebrovascular disease by inducing autophagy of abnormal mitochondria
US20220273814A1 (en) C60 glutathione dopa and methods
US20220265707A1 (en) Antimicrobial nano-surfactant and methods
JP2020079240A (en) Medicine for preventing or treating parkinson disease
CN117295704A (en) Fullerene phosphate polydigalloyl and method
US20220226500A1 (en) Fullerenic ellagic luteolin and methods
Ma et al. Peptidomimetic-liganded gold nanoclusters for controlled iron delivery and synergistic suppression of tumor growth
Gurunathan et al. Silver nanoparticle enhances secretion of exosomes in SH-SY5Y cells: potential therapeutic strategy for human neuroblastoma cancer
WO2022159332A1 (en) Fullerenic ellagic luteolin and methods
Wu et al. Inhalable iron redox cycling powered nanoreactor for amplified ferroptosis-apoptosis synergetic therapy of lung cancer
Li et al. The application of nanoparticles based on ferroptosis in cancer therapy
Sun et al. Cancer stem cells of head and neck squamous cell carcinoma; distance towards clinical application; a systematic review of literature
Tan Augumenting the anticancer properties of l-phenylalanine functionalized nanoscopic porous amino acid mimics (Nano-pPAAMs)
US20220211869A1 (en) Phenylalanine functionalised mesoporous silica nanoparticles as drug-free nanotherapeutics or bioactive nanocarrier for anti-cancer applications
US20220273804A1 (en) Fullerene gaba dopa and methods
CN114558148B (en) Preparation method and application of tumor switch-type nano phototherapy system

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22771889

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2022771889

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2022771889

Country of ref document: EP

Effective date: 20231016

WWE Wipo information: entry into national phase

Ref document number: 11202306694V

Country of ref document: SG

WWE Wipo information: entry into national phase

Ref document number: 202280032624.4

Country of ref document: CN