WO2022195035A3 - Process of obtaining a purified rubisco preparation from a photosynthetic material - Google Patents

Process of obtaining a purified rubisco preparation from a photosynthetic material Download PDF

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Publication number
WO2022195035A3
WO2022195035A3 PCT/EP2022/057046 EP2022057046W WO2022195035A3 WO 2022195035 A3 WO2022195035 A3 WO 2022195035A3 EP 2022057046 W EP2022057046 W EP 2022057046W WO 2022195035 A3 WO2022195035 A3 WO 2022195035A3
Authority
WO
WIPO (PCT)
Prior art keywords
obtaining
rubisco
preparation
photosynthetic material
photosynthetic
Prior art date
Application number
PCT/EP2022/057046
Other languages
French (fr)
Other versions
WO2022195035A2 (en
Inventor
Ricardo Manuel De Seixas Boavida Ferreira
Ana Isabel GUSMÃO LIMA
Sabrina DOS SANTOS OLIVEIRA
Madalena DE MATOS ÁGUAS GRÁCIO
Giovanni DEL FRARI
Original Assignee
Instituto Superior De Agronomia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Superior De Agronomia filed Critical Instituto Superior De Agronomia
Publication of WO2022195035A2 publication Critical patent/WO2022195035A2/en
Publication of WO2022195035A3 publication Critical patent/WO2022195035A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
    • A23J1/007—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials from leafy vegetables, e.g. alfalfa, clover, grass
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Water Supply & Treatment (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

A process of obtaining a RuBisCO preparation from photosynthetic material, wherein the process comprises the steps of: - lysing said photosynthetic material to extract RuBisCO accompanied by a fraction of chlorophylls of said photosynthetic material;- performing precipitation with sodium phytate and/or calcium chloride; - obtaining a phytate-calcium-RuBisCO insoluble ternary complex; and - performing solubilization of said complex.
PCT/EP2022/057046 2021-03-17 2022-03-17 Process of obtaining a purified rubisco preparation from a photosynthetic material WO2022195035A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT117122A PT117122A (en) 2021-03-17 2021-03-17 PROCESS FOR OBTAINING PURE RUBISCO FROM A PHOTOSYNTHETIC MATERIAL
PT117122 2021-03-17

Publications (2)

Publication Number Publication Date
WO2022195035A2 WO2022195035A2 (en) 2022-09-22
WO2022195035A3 true WO2022195035A3 (en) 2022-11-24

Family

ID=81307412

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2022/057046 WO2022195035A2 (en) 2021-03-17 2022-03-17 Process of obtaining a purified rubisco preparation from a photosynthetic material

Country Status (2)

Country Link
PT (1) PT117122A (en)
WO (1) WO2022195035A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130160166A1 (en) * 2011-11-18 2013-06-20 University Of Central Florida Research Foundation, Inc. Oral insulin therapy
WO2020148704A1 (en) * 2019-01-18 2020-07-23 R. J. Reynolds Tobacco Company Plant-derived rubisco protein purification
WO2020260831A1 (en) * 2019-06-27 2020-12-30 Institut National Des Sciences Appliquees De Toulouse Process for the production of rubisco from a plant biomass
WO2021007484A1 (en) * 2019-07-11 2021-01-14 Plantible Foods, Inc. Process for isolating a high purity protein preparation from plant material and products thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4268632A (en) 1979-09-24 1981-05-19 Leaf Proteins, Inc. Process for isolation of ribulose 1,5-diphosphate carboxylase from plant leaves
US4400471A (en) 1980-11-03 1983-08-23 Johal Sarjit S Preparation and crystallization of Fraction I protein from plant sources
WO2011078671A1 (en) 2009-12-22 2011-06-30 Nizo Food Research B.V. Process for isolating a dechlorophylllized rubisco preparation from a plant material
EP2934187B1 (en) 2012-12-24 2019-07-31 Stichting Wageningen Research Economical process for the isolation of functional protein from plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130160166A1 (en) * 2011-11-18 2013-06-20 University Of Central Florida Research Foundation, Inc. Oral insulin therapy
WO2020148704A1 (en) * 2019-01-18 2020-07-23 R. J. Reynolds Tobacco Company Plant-derived rubisco protein purification
WO2020260831A1 (en) * 2019-06-27 2020-12-30 Institut National Des Sciences Appliquees De Toulouse Process for the production of rubisco from a plant biomass
WO2021007484A1 (en) * 2019-07-11 2021-01-14 Plantible Foods, Inc. Process for isolating a high purity protein preparation from plant material and products thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BARBEAU W E ET AL: "RIBULOSE BISPHOSPHATE CARBOXYLASE-OXYGENASE RUBISCO FROM GREEN LEAVES POTENTIAL AS A FOOD PROTEIN", FOOD REVIEWS INTERNATIONAL, TAYLOR & FRANCIS, PHILADELPHIA, USA, vol. 4, no. 1, 1 January 1988 (1988-01-01), pages 93 - 127, XP008162475, ISSN: 8755-9129, DOI: 10.1080/87559128809540823 *
DI STEFANO ELISA ET AL: "Plant RuBisCo: An Underutilized Protein for Food Applications", JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, vol. 95, no. 8, 1 August 2018 (2018-08-01), DE, pages 1063 - 1074, XP055952975, ISSN: 0003-021X, Retrieved from the Internet <URL:https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Faocs.12104> DOI: 10.1002/aocs.12104 *
KRISHNAN H B ET AL: "A rapid method for depletion of Rubisco from soybean (Glycine max) leaf for proteomic analysis of lower abundance proteins", PHYTOCHEMISTRY, ELSEVIER, AMSTERDAM , NL, vol. 70, no. 17-18, 1 December 2009 (2009-12-01), pages 1958 - 1964, XP026789107, ISSN: 0031-9422, [retrieved on 20090918], DOI: 10.1016/J.PHYTOCHEM.2009.08.020 *

Also Published As

Publication number Publication date
PT117122A (en) 2022-11-30
WO2022195035A2 (en) 2022-09-22

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