WO2022185265A1 - Une composition activant la fermentation pour faciliter l'utilisation ou la réutilisation du lavage résiduel - Google Patents

Une composition activant la fermentation pour faciliter l'utilisation ou la réutilisation du lavage résiduel Download PDF

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Publication number
WO2022185265A1
WO2022185265A1 PCT/IB2022/051916 IB2022051916W WO2022185265A1 WO 2022185265 A1 WO2022185265 A1 WO 2022185265A1 IB 2022051916 W IB2022051916 W IB 2022051916W WO 2022185265 A1 WO2022185265 A1 WO 2022185265A1
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WIPO (PCT)
Prior art keywords
fermentation
condensate water
composition
concentration
enhancing composition
Prior art date
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PCT/IB2022/051916
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English (en)
Inventor
Dr. Sujata OJHA
Dr. Anirban PAL
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Praras Biosciences Pvt Ltd
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Publication date
Application filed by Praras Biosciences Pvt Ltd filed Critical Praras Biosciences Pvt Ltd
Publication of WO2022185265A1 publication Critical patent/WO2022185265A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms

Definitions

  • the present invention relates to a fermentation enhancing composition, which aids in the use or reuse of the spent wash.
  • the invention in particular relates to a composition, which enhances the yeast growth and aids in fermentation of molasses using the condensate water obtained from the spent wash.
  • the conventional methods of spent wash treatment involve bio- methanation followed by irrigation or bio-methanation and secondary treatment followed by irrigation or disposal in surface water which leads to surface water contamination.
  • bio-composting is another traditional treatment method to treat the effluent
  • time and space are the main limitations for such industries to enable an anaerobic digestion followed by an aerobic digestion of the effluent.
  • the traditional method is feasible, the effluent colour and solids of the spent wash is not totally reduced.
  • the Central Pollution Control Board does not encourage the disposal of large volume for treatment and land application or ferti-irrigation, since this method of treatment pollutes the soil and groundwater.
  • An alternative option for effluent treatment is disposal by dilution in sea or estuary after bio-methanation.
  • the CPCB has enforced on minimizing pollutants from distillery to zero discharge. Additionally, the board has prepared the ‘Guidelines on Techno-Economic Feasibility of implementation of Zero Liquid Discharge (ZLD) for Water Polluting Industries’.
  • ZLD Zero Liquid Discharge
  • the recommended methods to achieve ZLD includes a combination of bio-methanation, reverse osmosis, multi-effect evaporator, incineration, spray drying or bio-compositing, etc.
  • the patent application “CN110819546A” entitled “ Method for recycling and reusing evaporative condensation liquid to produce yeast ” disclose a method for recycling and reusing evaporative condensation liquid to produce yeast.
  • an evaporative condensation liquid is adsorbed by resin, followed by mixing with a raw liquid to be reused in yeast production.
  • the growth of yeast in recycled evaporative condensation liquid is same as the growth of yeast in tap water with no major difference.
  • Volatile acids act as a major inhibitor of growth of yeast in the evaporative condensation liquid and upon the absorption of the volatile acids in the resin, they are subjected to heat-elution, the volatile acid is recovered by distillation and the rectifying residual liquid can be used as primary yeast washing water.
  • the recycle process of the evaporative condensation liquid and the high-density culture process are connected in series to achieve a purpose of no waste discharge.
  • the method provides a solution for high cost treatment of evaporative condensation liquid using a simple process. Further, reduces wastage of water resources and requires low equipment investment as well as an industrial promotion and application value.
  • the patent application “CN108928943A” entitled “ Method for treating alcohol waste liquid discharged from molasses alcohol factory ” discloses a method for treating an alcohol waste liquid.
  • the method comprises the steps of separating the alcohol waste liquid discharged from the molasses alcohol factory by a primary membrane, concentrating the resulting solution by a forced circulation evaporator, followed by subjecting the concentrated liquid to drying, and preparing a solid granule product from the dried concentrated liquid by a granulator, subsequently separating a dilute solution obtained after the primary membrane separation by a secondary membrane and a tertiary membrane, treating the resulting concentrated liquid obtained by an evaporative crystallizer, and carrying out solid-liquid separation after the crystallization to obtain a mixed solid salt to be recycled and reusing the water obtained after the tertiary separation in a production process without discharging.
  • a series of membrane technologies are implemented to perform treatment in order to remove the chloride ions in the waste liquid and membrane technology concentrating is carried out without heating.
  • the cost is one tenth of that of steam heating, thus saving energy and implementing an in-expensive approach.
  • large amount of reclaimed water is reused in the production process.
  • the patent application “WO2017072787A1” entitled “ Method and apparatus for zero discharge treatment of industrial wastewater streams ” discloses a method and apparatus to achieve zero discharge of waste matter.
  • the method and apparatus is applicable for treatment of wastewater such as distillery spent wash which may be raw, or molasses spent wash or bio-methanated spent wash.
  • the process involves subjecting the spent wash to evaporation, treating with chemical adsorbent, followed by solid-liquid separation, concentrating the resulting viscosity building material and finally subjecting to binder treatment.
  • the invention mainly aims to the reduce viscosity building substances from said wastewater streams and further removal of such viscosity building substance so that about 80 to 90% water is recovered from said wastewater streams.
  • the method further provides solid by-products useful as fuel and soil conditioner components.
  • the patent application “IN2535DEL2014A” entitled “ Simultaneous decolonization and reduction in organic matter of molasses spent wash using novel fungal strain Curvularia androponis ” relates to the use of novel fungus Curvularia andropogonis for treating the spent wash or contaminated water in the distilleries industry.
  • the invention provides a safe, cost-effective, compact, easy to use and environmentally friendly alternative to issues such as the treatment and disposal of colored industrial effluent of distillery.
  • the invention provides the treatment of colored effluent with the novel fungus Curvularia andropogonis for decolourization and reduction of COD.
  • the patent application “WO2013055676A1” entitled “ Processes for producing fermentation products ” discloses processes for producing fermentation products from starch-containing material.
  • the process comprises the addition of a bacterial alpha-amylase, a raw starch hydrolyzing alpha-amylase and a carbohydrate-source generating enzyme during liquefaction.
  • the invention also relates to compositions appropriate for use in processes of the invention.
  • the patent application WO2009134964A3 entitled “Enhanced fermentation process using molasses’ ’ discloses the compositions and methods for enhanced fermentation of molasses using transglucosidase.
  • the enzyme hydrolyzes non-fermentable sugars such as raffinose or stachyose.
  • the method also involves addition of secondary enzyme to hydrolyze granular starch, proteins and residual starch in the molasses.
  • the patent application US8110383B2 entitled “ Fermentation process starting from cellulosic biomass and involving the recirculation of detoxified stillage into the process” discloses the method for preparing a target chemical derivable from cellulosic biomass.
  • the method comprises the steps of providing a cellulosic biomass, subjecting the cellulosic biomass to aqueous pretreatment, and aqueous hydrolysis, followed by fermentation under conditions in which at least a part of the fermentable sugars are fermented into a primary target chemical, subsequently separating the primary target chemical from the fermented hydrolysate to obtain a spent hydrolysate comprising inhibitory substances.
  • the resulting spent hydrolysate is detoxified using a detoxification catalyst which decreases the concentration of at least one of the inhibitory substances.
  • the detoxifying catalyst is selected from the group consisting of wild type, mutant and recombinant filamentous fungi.
  • recirculating a portion of the detoxified spent hydrolysate after further purification is selected from the group consisting of wild type, mutant and recombinant filamentous fungi.
  • the invention overcomes the drawbacks of the existing prior arts by providing a suitable remedy to treat distillery effluents to achieve ZLD.
  • the present invention discloses a fermentation enhancing composition, which aids in the use or reuse of the spent wash.
  • the spent wash is heated continuously to collect the condensate water.
  • the said water is used for the dilution of molasses for yeast growth and fermentation, thus reducing the consumption of groundwater.
  • the fermentation enhancer composition comprises amylase at a concentration in the range between 25% and 40%, protease at a concentration in the range between 15% and 20%, and maltodextrin at a concentration in the range between 40% and 60%.
  • the composition aids in increasing the yeast cell count during fermentation and thus acts as a fermentation enhancer for diluting the molasses and reuse the condensate water for consecutive batches for yeast cell proliferation.
  • the condensate water treated with the fermentation enhancing composition increases the yeast cell viability and decreases the accumulation of volatile acids compared to the condensate water without the fermentation enhancer composition.
  • the preliminary laboratory results demonstrated an increase in the yeast cell count, cell viability, alcohol yield, fermentation efficiency due to the presence of the fermentation enhancer composition at a dosage of 5 ppm and a nutrient such as diammonium phosphate at a concentration of 200 ppm in the wort made of condensate water when compared to the control sample comprising a wort made of the condensate water alone.
  • the accumulation of volatile acids is more when condensate water or control alone is used in 5 subsequent cycles of fermentation and the accumulation of volatile acids decreases when the wort made of condensate water and fermentation enhancers are used under the same conditions.
  • the invention mainly relates to the recovery of condensate water from spent wash and reuse of the condensate water in diluting molasses for fermentation, thus conserving ground or freshwater for human use.
  • the advantage of the present invention is to conserve water and simultaneously reduce effluent.
  • the invention plays a role in achieving zero discharge of effluents, aids in the conservation of ground or freshwater, provides a cleaner and eco-friendly technology to enhance the value of the waste or effluents.
  • Figure 1 tabulates the composition of the fermentation enhancer and their weight percentage.
  • Figure 2 illustrates a graph depicting the growth pattern of yeast during fermentation.
  • Figure 3 illustrates a graph depicting the viability of yeast cells during fermentation.
  • Figure 4 illustrates a graph depicting the accumulation of volatile acids in 5 subsequent cycles during fermentation.
  • Figure 5 illustrates a graph depicting the fermentation efficiency in 5 subsequent cycles during fermentation.
  • Spent wash refers to a residual liquid waste generated during alcohol production.
  • molasses is a byproduct obtained during the process of making sugar.
  • the invention discloses a fermentation enhancing composition, which aids in the use or reuse of the spent wash.
  • the spent wash is heated continuously to collect condensate water thus eliminating the need for groundwater to dilute molasses during fermentation.
  • Figure 1 tabulates the composition of the fermentation enhancers and their weight percentage.
  • the fermentation enhancing composition comprises an enzyme amylase at a concentration in the range between 25% and 40%, an enzyme protease at a concentration in the range between 15% and 20%, and a polysaccharide maltodextrin at a concentration in the range between 40% and 60%.
  • the composition aids in increasing the yeast cell count during fermentation and thus acts as a fermentation enhancer when added to the condensate water for diluting the molasses and reuse the condensate water for consecutive batches.
  • the condensate water treated with the fermentation enhancing composition increases the yeast cell viability and decreases the accumulation of volatile acids compared to the condensate water without the fermentation enhancer composition.
  • Example 1 Fermentation of molasses using condensate water for 5 consecutive cycles with fermentation enhancer composition
  • the molasses fermentation is carried out for 5 consequent cycles and the wort is prepared at a dilution of l:3w/v i.e., one part of molasses is diluted with 3 parts of condensate water.
  • Commercially cultured yeast is used for fermentation.
  • the nutrient such as Diammonium phosphate (DAP) is used at a concentration of 200 ppm.
  • the enzymes to facilitate fermentation is provided by the fermentation enhancer composition at a concentration of 5 ppm.
  • Figure 2 illustrates a graph depicting the growth pattern of yeast during fermentation.
  • the growth pattern of yeast is compared using groundwater, condensate water, condensate water with fermentation enhancer composition at 5 ppm.
  • the yeast cell count increased up to 4.6% during the fermentation in the wort made of condensate water with fermentation enhancer composition at a dosage of 5 ppm when compared to the sample comprising the wort made of the condensate water alone.
  • Figure 3 illustrates a graph depicting the viability of yeast cell during fermentation.
  • the viability of yeast cells is compared using groundwater, condensate water, condensate water with fermentation enhancer composition at 5 ppm.
  • the viability of the yeast cell increased to 5.8% during the fermentation in the wort made of condensate water with fermentation enhancer composition at a dosage of 5 ppm when compared to the sample comprising the wort made of the condensate water alone.
  • the fermentation is carried out by reusing the condensate water in 5 consecutive cycles of fermentation.
  • the parameters of the molasses and condensate water is checked at the commencement of each cycle and at the end of each cycle.
  • the parameter of the molasses and condensate water before initiating the fermentation is as follows: Molasses:
  • the first cycle is initiated with a batch size of 30 liters, DAP at a concentration of 200 ppm and fermentation enhancer composition at a concentration of 5 ppm is used.
  • the initial parameters before the start of the batch are as below: Brix: 20.9 pH: 4.34 Sp.
  • Figure 4 illustrates a graph depicting the accumulation of volatile acids in 5 subsequent cycles during fermentation.
  • the concentration of VA in the condensate water is 3180 ppm and the pH of the condensate water is 3.20.
  • the condensate water from cycle 1 is used for dilution of molasses for cycle 2 with a batch size of 15 liters, the initial and final parameters in each cycle are measured.
  • the concentration of VA in condensate water is 2400 ppm and pH is 3.11.
  • the condensate water from cycle 2 is used in cycle 3 with a batch size of 8 liters.
  • the concentration of VA in condensate water from cycle 3 is 1714 ppm and pH is 3.20.
  • the condensate water from cycle 3 is reused for cycle 4 with a batch size of 5 liters.
  • the concentration of VA in condensate water from cycle 4 is 1337 ppm and the pH is 3.02.
  • the condensate water from cycle 4 is reused for cycle 5 with a batch size of 3 liters.
  • the concentration of VA in condensate water from cycle 5 is 1380 ppm with a pH of 3.09. Thus, a reduction in volatile acids is observed.
  • the accumulation of volatile acids is found to be 2900 ppm, which is relatively more in fermentation when condensate water or control alone is used in 5 subsequent cycles compared to the accumulation of volatile acids which decreased to 1380 ppm (cycle 5) from 4000 ppm (cycle 1) when the wort made of condensate water and fermentation enhancers are used under the same conditions.
  • Figure 5 illustrates a graph depicting the fermentation efficiency in 5 subsequent cycles during fermentation.
  • the fermentation efficiency increased to 13.1% and the alcohol yield increased to 12.3% during the fermentation in a wort made of condensate water with fermentation enhancer composition at a dosage of 5 ppm used in 5 subsequent cycles when compared to the sample comprising the wort made of only condensate water.
  • the addition of the fermentation enhancer composition to the condensate water maintains the fermentation efficiency same as groundwater i.e. 90+1%
  • the main advantage of the present invention is the recovery of condensate water from the spent wash to reduce the treatment and disposal of effluents.
  • the condensate water is used for diluting molasses during fermentation thus replacing the consumption of conserving ground or freshwater.
  • the invention is useful in conserving water and simultaneously reduce effluent at source. Apart from the economic impact in the plant, it also has a major sociological impact.
  • the invention is mainly useful in achieving zero discharge of effluents, aids in the conservation of ground or freshwater, provides a cleaner and environmentally friendly technology by enhancing the value of the waste or effluents.

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  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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Abstract

La présente invention concerne une composition activant la fermentation, facilitant l'utilisation ou la réutilisation de la solution de lavage résiduelle. La solution de lavage résiduelle est chauffée en continu pour recueillir l'eau de condensat. L'eau est utilisée pour la dilution de la mélasse pour la croissance et la fermentation de la levure. La composition d'activateur de fermentation comprend de l'amylase à une concentration comprise entre 25 % et 40 %, protéase à une concentration comprise entre 15 % et 20 %, et de la maltodextrine à une concentration comprise entre 40 % et 60 %. La composition aide à augmenter le nombre de cellules de levure pendant la fermentation et agit ainsi en tant qu'activateur de fermentation lorsqu'elle est utilisée avec de l'eau de condensat pour diluer la mélasse et réutiliser l'eau de condensat pour des lots consécutifs pour la prolifération de cellules de levure.
PCT/IB2022/051916 2021-03-05 2022-03-04 Une composition activant la fermentation pour faciliter l'utilisation ou la réutilisation du lavage résiduel WO2022185265A1 (fr)

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IN202141009260 2021-03-05
IN202141009260 2021-03-05

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013055676A1 (fr) * 2011-10-11 2013-04-18 Novozymes North America, Inc. Procédés de production de produits de fermentation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013055676A1 (fr) * 2011-10-11 2013-04-18 Novozymes North America, Inc. Procédés de production de produits de fermentation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "TECHNICAL INFORMATION SHEET: DIAMMONIUM PHOSPHATE -YEAST NUTRIENT", MURPHY & SON LIMITED, 1 September 2018 (2018-09-01), XP055967300, Retrieved from the Internet <URL:https://www.murphyandson.co.uk/wp-content/uploads/2018/09/Diammonium-Phosphate-powder-Rev-3.pdf> [retrieved on 20221003] *
SANGAVE, PREETI C. ET AL.: "Enhancement in biodegradability of distillery wastewater using enzymatic pretreatment", JOURNAL OF ENVIRONMENTAL MANAGEMENT, vol. 78.1, 2006, pages 77 - 85, XP024946281, DOI: 10.1016/j.jenvman.2005.03.012 *

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