WO2022171606A3 - Methods for base-level detection of methylation in nucleic acids - Google Patents

Methods for base-level detection of methylation in nucleic acids Download PDF

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Publication number
WO2022171606A3
WO2022171606A3 PCT/EP2022/052979 EP2022052979W WO2022171606A3 WO 2022171606 A3 WO2022171606 A3 WO 2022171606A3 EP 2022052979 W EP2022052979 W EP 2022052979W WO 2022171606 A3 WO2022171606 A3 WO 2022171606A3
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WO
WIPO (PCT)
Prior art keywords
methods
nucleic acids
methylation
base
level detection
Prior art date
Application number
PCT/EP2022/052979
Other languages
French (fr)
Other versions
WO2022171606A2 (en
Inventor
Frank Bergmann
Shwu shin CHANG
Peter CRISALLI
Abre De Beer
Dieter Heindl
Omid KHAKSHOOR
David L. PENKLER
Jo-Anne PENKLER
Martin Ranik
Meng Taing
Original Assignee
F. Hoffmann-La Roche Ag
Roche Diagnostics Gmbh
Roche Sequencing Solutions, Inc.
Kapa Biosystems, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F. Hoffmann-La Roche Ag, Roche Diagnostics Gmbh, Roche Sequencing Solutions, Inc., Kapa Biosystems, Inc. filed Critical F. Hoffmann-La Roche Ag
Priority to JP2023547833A priority Critical patent/JP2024506899A/en
Priority to EP22705528.2A priority patent/EP4291677A2/en
Priority to CN202280013794.8A priority patent/CN116806266A/en
Publication of WO2022171606A2 publication Critical patent/WO2022171606A2/en
Publication of WO2022171606A3 publication Critical patent/WO2022171606A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention includes improved methods and compositions for detecting methylation in nucleic acids. In particular, the disclosure is directed to methods of converting 5-hydroxymethyl cytosine (5hmC) and/or 5-methylcytosine (5mC) to Thymine (T). In addition, the disclosure is also directed to methods of detecting 5hmC and/or 5mC in a sample.
PCT/EP2022/052979 2021-02-09 2022-02-08 Methods for base-level detection of methylation in nucleic acids WO2022171606A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2023547833A JP2024506899A (en) 2021-02-09 2022-02-08 Method for base level detection of methylation in nucleic acids
EP22705528.2A EP4291677A2 (en) 2021-02-09 2022-02-08 Methods for base-level detection of methylation in nucleic acids
CN202280013794.8A CN116806266A (en) 2021-02-09 2022-02-08 Method for detection of methylated base level in nucleic acids

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US202163147307P 2021-02-09 2021-02-09
US63/147,307 2021-02-09
US202163150019P 2021-02-16 2021-02-16
US63/150,019 2021-02-16
US202163191079P 2021-05-20 2021-05-20
US63/191,079 2021-05-20

Publications (2)

Publication Number Publication Date
WO2022171606A2 WO2022171606A2 (en) 2022-08-18
WO2022171606A3 true WO2022171606A3 (en) 2022-10-06

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EP (1) EP4291677A2 (en)
JP (1) JP2024506899A (en)
WO (1) WO2022171606A2 (en)

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Publication number Publication date
EP4291677A2 (en) 2023-12-20
WO2022171606A2 (en) 2022-08-18
JP2024506899A (en) 2024-02-15

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