WO2022154188A1 - Nouveau variant de polycétide synthase et procédé de production de xmp ou gmp l'utilisant - Google Patents
Nouveau variant de polycétide synthase et procédé de production de xmp ou gmp l'utilisant Download PDFInfo
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- WO2022154188A1 WO2022154188A1 PCT/KR2021/005073 KR2021005073W WO2022154188A1 WO 2022154188 A1 WO2022154188 A1 WO 2022154188A1 KR 2021005073 W KR2021005073 W KR 2021005073W WO 2022154188 A1 WO2022154188 A1 WO 2022154188A1
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- polypeptide
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
Definitions
- the vector used in the present application is not particularly limited, and any vector known in the art may be used.
- Examples of commonly used vectors include plasmids, cosmids, viruses and bacteriophages in a natural or recombinant state.
- pWE15, M13, MBL3, MBL4, IXII, ASHII, APII, t10, t11, Charon4A, and Charon21A may be used as phage vectors or cosmid vectors, and pDZ-based, pBR-based, and pUC-based plasmid vectors may be used.
- Another aspect of the present application includes a variant of the present application or a polynucleotide of the present application, Corynebacterium stationis ( Corynebacterium stationis ) It is to provide a strain.
- the strain of the present application is a microorganism having naturally polyketide synthase or XMP or GMP production ability, or a mutant of the present application or a polynucleotide ( Or a vector comprising the polynucleotide) introduced and / or XMP or GMP production ability may be a microorganism endowed, but is not limited thereto.
- the modification of the amino acid sequence or polynucleotide sequence of 4) and 5) above may include deletion, insertion, non-conservative or conservative substitution of the amino acid sequence of the polypeptide or the polynucleotide sequence encoding the polypeptide to enhance the activity of the polypeptide;
- a combination thereof may result in sequence mutation, or replacement with an amino acid sequence or polynucleotide sequence improved to have stronger activity or an amino acid sequence or polynucleotide sequence improved to increase activity, but is not limited thereto.
- the replacement may be specifically performed by inserting a polynucleotide into a chromosome by homologous recombination, but is not limited thereto.
- the vector used may further include a selection marker for confirming whether or not the chromosome is inserted.
- the selection marker is the same as described above.
- a plasmid (pDCM2, FIG. 1, SEQ ID NO: 11) for the insertion and replacement of genes in the Corynebacterium chromosome was designed, and the plasmid was synthesized using the Gene-synthesis service of Bionics Co., Ltd.
- a plasmid was designed to include a restriction enzyme that is easy to use for cloning with reference to a generally known sacB system related paper [Gene, 145 (1994) 69-73].
- the thus synthesized pDCM2 plasmid has the following characteristics.
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Abstract
La présente invention concerne un nouveau variant de polycétide synthase, une souche de Corynebacterium stationis comprenant le variant, et un procédé de production de XMP ou GMP à l'aide de la souche.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020210006130A KR102266232B1 (ko) | 2021-01-15 | 2021-01-15 | 신규한 폴리케타이드 신타제 변이체 및 이를 이용한 xmp 또는 gmp 생산 방법 |
| KR10-2021-0006130 | 2021-01-15 |
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| Publication Number | Publication Date |
|---|---|
| WO2022154188A1 true WO2022154188A1 (fr) | 2022-07-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/KR2021/005073 WO2022154188A1 (fr) | 2021-01-15 | 2021-04-22 | Nouveau variant de polycétide synthase et procédé de production de xmp ou gmp l'utilisant |
Country Status (2)
| Country | Link |
|---|---|
| KR (1) | KR102266232B1 (fr) |
| WO (1) | WO2022154188A1 (fr) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120096581A1 (en) * | 2000-12-15 | 2012-04-19 | The Salk Institute For Biological Studies | Methods of producing polyketide synthase mutants and compositions and uses thereof |
| WO2015134807A1 (fr) * | 2014-03-06 | 2015-09-11 | Salk Institute For Biological Studies | Variants de polycétide synthase et leurs utilisations |
| CN106635939A (zh) * | 2016-09-26 | 2017-05-10 | 江南大学 | 一种产多种l‑氨基酸的基因工程菌及应用 |
| KR101950141B1 (ko) * | 2018-08-01 | 2019-02-19 | 씨제이제일제당 (주) | 신규 아데닐로석시네이트 신세타아제 및 이를 이용한 퓨린 뉴클레오티드 생산방법 |
-
2021
- 2021-01-15 KR KR1020210006130A patent/KR102266232B1/ko active IP Right Grant
- 2021-04-22 WO PCT/KR2021/005073 patent/WO2022154188A1/fr active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120096581A1 (en) * | 2000-12-15 | 2012-04-19 | The Salk Institute For Biological Studies | Methods of producing polyketide synthase mutants and compositions and uses thereof |
| WO2015134807A1 (fr) * | 2014-03-06 | 2015-09-11 | Salk Institute For Biological Studies | Variants de polycétide synthase et leurs utilisations |
| CN106635939A (zh) * | 2016-09-26 | 2017-05-10 | 江南大学 | 一种产多种l‑氨基酸的基因工程菌及应用 |
| KR101950141B1 (ko) * | 2018-08-01 | 2019-02-19 | 씨제이제일제당 (주) | 신규 아데닐로석시네이트 신세타아제 및 이를 이용한 퓨린 뉴클레오티드 생산방법 |
Non-Patent Citations (2)
| Title |
|---|
| AISWARYA GIRIJA; MALLIKA VIJAYANATHAN; MUR LUIS A.; SONIYA EPPURATHU VASUDEVAN: "Ectopic expression and functional characterization of type III polyketide synthase mutants fromEmblica officinalisGaertn", PLANT CELL REPORTS, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 35, no. 10, 12 July 2016 (2016-07-12), Berlin/Heidelberg, pages 2077 - 2090, XP036056118, ISSN: 0721-7714, DOI: 10.1007/s00299-016-2020-0 * |
| DATABASE Protein NCBI; 18 August 2016 (2016-08-18), ANONYMOUS : "polyketide synthase [Corynebacterium stationis]", XP055950873, Database accession no. WP_066796749 * |
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| KR102266232B1 (ko) | 2021-06-17 |
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