WO2022126845A1

WO2022126845A1 - Blood viscoelasticity detection device and detection method

Info

Publication number: WO2022126845A1
Application number: PCT/CN2021/074701
Authority: WO
Inventors: 蔡泳; 周奇; 刘勇; 周光银; 李欣
Original assignee: 重庆南方数控设备股份有限公司
Priority date: 2020-12-18
Filing date: 2021-02-01
Publication date: 2022-06-23
Also published as: CN114646588A

Abstract

A blood viscoelasticity detection device and detection method. The detection device comprises a pressure main pipe (1) one end of which communicates with the atmosphere and is an open end. The other end of the pressure main pipe (1) is a closed end and is branched with a pressure branch pipe (2). The pressure branch pipe (2) is provided with an axially movable pressure pump (3) and forms a seal. The open end of the pressure main pipe (1) is provided with a blood sample (4) and forms a seal. A closed pressure chamber (5) is formed between the blood sample (4) and the pressure pump (3). A pressure sensor (6) is provided in the pressure chamber (5). The detection method is implemented on the basis of the aforementioned device. The pressure pump (3) exerts an action force on the blood sample (4) during detection. When the displacement of the pressure pump (3) is the same, due to the differences in shear stress of different blood samples (4), the deformation amounts of different blood samples (4) are also different, so that the volume of the pressure chamber (5) is different, and thus pressure values in the pressure chamber (5) are different. By means of measuring and comparing the pressure values in the pressure chamber (5), the states of the elastic moduli of the blood samples (4) may be characterized. Finally, the relationship between pressure and time is plotted to facilitate the comparison of different viscoelasticities.

Description

A kind of detection device and detection method of blood viscoelasticity

technical field

The invention relates to the field of viscoelasticity analysis, in particular to a blood viscoelasticity detection device and a detection method.

Background technique

Thromboelastometry technology is to study the coagulation process by continuously monitoring the changes of blood viscoelasticity during blood coagulation. In the existing device, the blood coagulation process and viscoelastic changes are reflected by measuring the firmness of blood clots, as shown in FIG. , the end of the metal shaft 106 is fixed with a pin 103 and the pin 103 is inserted into the blood sample 101, the metal shaft 106 is periodically rotated around its vertical axis by the sensitive spring 108; the movement of the pin 103 can detect the firmness of the blood clot, and also The optical detection device 105 can be continuously recorded by beam deflection, and the blood clot firmness versus time can be plotted. However, the structure of this kind of device is too complicated; the processing of the pin 103 and the metal shaft 106 is difficult and the cost is high, and the whole device must work in a stable working environment.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a blood viscoelasticity detection device and a detection method with a simple structure and convenient measurement, aiming at the complex structure of the blood viscoelasticity detection device in the prior art.

In order to achieve the above-mentioned purpose of the invention, according to one aspect of the present invention, the following technical solutions are provided:

A blood viscoelasticity detection device, comprising a pressure main pipe whose one end is communicated with the atmosphere and is an open end, the other end of the pressure main pipe is a closed end and is provided with a pressure branch pipe, the pressure branch pipe is provided with a pressure pump and forms a seal, and the open end of the pressure main pipe is The end is provided with a blood sample and forms a seal, and a closed pressure chamber is formed between the blood sample and the pressure pump; a pressure sensor is arranged in the pressure chamber.

In the present invention adopting the above technical solutions, according to the principle of fluid mechanics, when the fluid flows in the pipeline, the shear stress inside the fluid will prevent the fluid from flowing, and under low-speed flow, the fluid exhibits elastic characteristics; Acting force to ensure that the acting force does not make the fluid move or the flow rate is as close to zero as possible. At this time, the fluid will produce corresponding deformation, and the elasticity of the fluid can be represented by the deformation value; and this device uses the principle of fluid mechanics, through the pressure A pressure pump is installed in the branch pipe, and the air in the pressure chamber is compressed by the movement of the pressure pump, and then a uniform force is applied to the blood sample through the air, so that the blood sample is deformed, and at the same time, it is ensured that the force does not push the blood sample. ; In addition, when testing, the pressure pump is used to exert a force on the blood sample. In the case of the same displacement of the pressure pump, due to the different shear stress of different blood samples, the deformation of different blood samples is also different, which makes the pressure chamber The volume is different, so the pressure value in the pressure chamber is different. By measuring and comparing the pressure value in the pressure chamber, the state of the elastic modulus of the blood sample can be characterized. The device separates the pressure main pipe and the pressure branch pipe to distinguish the area where the pressure pump and the blood sample are located, which facilitates the pressure pump to provide a small force to ensure that the blood sample does not move in the pressure main pipe.

Further, the axial direction of the pressure main pipe is in the horizontal direction. The pressure main tube can be set in the vertical or horizontal direction, as long as the blood sample does not move in the pressure main tube; here, the pressure main tube is set horizontally to prevent the blood sample from being affected by its own gravity. Stress governs sports conditions.

Further, the ratio of the radius of the pressure main pipe and the pressure branch pipe is 1-5.5. Reasonably set the relative value of the radius of the pressure main pipe and the pressure branch pipe to facilitate fine-tuning of the force on the blood sample.

Further, a constant temperature device is provided on both the pressure main pipe and the pressure branch pipe. The constant temperature device ensures that the temperature of the blood sample and the pressure chamber is consistent throughout the measurement process, reducing variables and improving the accuracy of the test results.

Further, a detection cavity is provided on the pressure branch pipe, and the end of the detection cavity is fixedly connected with the pressure sensor to form a seal. Reasonably set the position of the detection chamber so that the blood sample can be loaded into the pressure main tube before the experiment starts; protect the integrity of the loaded blood sample and avoid the blood sample sticking to the pressure sensor, thus affecting the detection result.

Further, the pressure pump is connected with a control system, and the pressure pump performs periodic reciprocating movement along the axial direction of the pressure branch pipe under the control of the control system. Since the blood sample is in the process of gradual coagulation, the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. , to reflect the change in viscoelasticity of blood samples over time.

Further, when the pressure pump moves, the volume change of the pressure chamber does not exceed 20%. Reasonably set the volume change of the pressure chamber to reasonably set the movement range of the pressure pump, and then set the force on the blood sample reasonably, to avoid the blood sample moving in the pressure main tube, and to facilitate the measurement and the adjustment of the pressure pump position.

Further, the maximum change in the volume of the pressure chamber is 2ul-20ul, and the dosage of the blood sample is 20ul-60ul. The maximum change in the volume of the pressure chamber is set corresponding to the amount of the blood sample, which avoids the movement of the blood sample in the pressure main tube and facilitates the movement of the pressure pump.

Further, during the movement of the pressure pump, the relative position of the blood sample and the pressure main pipe does not change; the movement time of one cycle of the pressure pump is 0.5s to 10s, and the stay time of the pressure pump moving to the minimum volume of the pressure chamber is six minutes. one cycle. Reasonably set the movement period of the pressure pump, and make the pressure pump stop at the minimum volume of the pressure chamber, so as to facilitate the pressure sensor to measure the air pressure in the pressure chamber.

According to another aspect of the present invention, a method for detecting blood viscoelasticity is provided, including a blood viscoelasticity detecting device. In each movement cycle of the pressure pump, the starting point and the end point of the pressure pump moving in the pressure branch remain unchanged. The pressure pump performs periodic movement of the pressure branch pipe; the pressure sensor measures the pressure value of the pressure chamber in each movement cycle of the pressure pump, and the measured value is the pressure value at the minimum volume of the pressure chamber; the control system is connected with a signal processing system , the signal processing system analyzes the pressure value measured by the pressure sensor and draws a curve of the pressure changing with time. Since the blood sample is in the process of gradual coagulation, the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. ; By maintaining the same movement position of the pressure pump in each cycle, the volume reduction of the cavity in the pressure branch pipe remains the same. Changes in the viscoelasticity of blood samples; draw the relationship curve between pressure and time to facilitate the comparison of different data to qualitatively analyze the viscoelasticity of blood samples.

Compared with the prior art, the beneficial effects of the present invention:

According to fluid mechanics, when a fluid flows in a pipe, the shear stress inside the fluid will prevent the fluid from flowing. Under low-speed flow, the fluid exhibits elastic characteristics; if a force is applied on one side of the static fluid, ensure that the force does not cause When the fluid moves or the flow rate is as close to zero as possible, the fluid will produce corresponding deformation, and the elasticity of the fluid can be represented by the deformation value. In this device, a pressure pump is set in the pressure branch pipe, and the pressure is adjusted by the movement of the pressure pump. The air in the cavity is compressed, and then a uniform force is applied to the blood sample through the air, so that the blood sample is deformed, and at the same time, the force does not push the blood sample to move; In the case of the same displacement of the pressure pump, due to the different shear stress of different blood samples, the deformation amount of different blood samples is also different, so that the volume of the pressure chamber is different, and the pressure value in the pressure chamber is different. The measurement and comparison of the pressure value in the pressure chamber can characterize the state of the elastic modulus of the blood sample. The separate pressure main pipe and pressure branch pipe of the device distinguish the area where the pressure pump and the blood sample are located, which facilitates the pressure pump to provide a small force to ensure that the blood sample does not move in the pressure main pipe;

Since the blood sample is in the process of gradual coagulation, the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. ; By maintaining the same movement position of the pressure pump in each cycle, the volume reduction of the cavity in the pressure branch pipe remains the same. Changes in the viscoelasticity of blood samples; draw the relationship curve between pressure and time to facilitate the comparison of different data to qualitatively analyze the viscoelasticity of blood samples.

Description of drawings:

The accompanying drawings forming a part of the present application are used to provide further understanding of the invention, and the schematic embodiments and descriptions of the present invention are used to explain the present invention and do not constitute an improper limitation of the present invention. In the attached image:

FIG. 1 shows the structure diagram of the blood viscoelasticity detection device in the prior art of the present invention.

FIG. 2 shows the structure diagram of the pressure pump in the first position of a blood viscoelasticity detection device and detection method of the present invention.

FIG. 3 shows the structure diagram of the pressure pump in the second position of a blood viscoelasticity detection device and detection method of the present invention.

Wherein, the above-mentioned drawings include the following reference signs:

1 pressure supervisor;

2 pressure branch pipes; 21 detection chambers;

3 pressure pumps; 4 blood samples; 5 pressure chambers; 6 pressure sensors.

Detailed ways

It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

In order to make those skilled in the art better understand the solutions of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only The embodiments are part of the present application, but not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the scope of protection of the present application.

1 and 2, a blood viscoelasticity detection device includes a pressure main pipe 1 whose one end is connected to the atmosphere and is an open end, and the other end of the pressure main pipe 1 is a closed end and is provided with a pressure branch pipe 2. The pressure branch pipe 2 is provided with There is an axially movable pressure pump 3 and forms a seal, the open end of the pressure main pipe 1 is provided with a blood sample 4 and forms a seal, and a closed pressure chamber 5 is formed between the blood sample 4 and the pressure pump 3; pressure sensor 6.

Preferably, the axial direction of the pressure main pipe 1 is in the horizontal direction.

Preferably, the ratio of the radius of the pressure main pipe 1 to the pressure branch pipe 2 is 1-5.5.

Preferably, a constant temperature device is provided on both the pressure main pipe 1 and the pressure branch pipe 2 .

Preferably, a detection cavity 21 is protruded from the pressure branch pipe 2, and the end of the detection cavity 21 is fixedly connected with the pressure sensor 6 to form a seal.

Preferably, the pressure pump 3 is connected with a control system, and under the control of the control system, the pressure pump 3 performs a reciprocating periodic movement along the axial direction of the pressure branch pipe 2 .

Preferably, when the pressure pump 3 is in motion, the volume change of the pressure chamber 5 is not more than 20%, preferably not more than 10%.

Preferably, the maximum volume change of the pressure chamber 5 is 2ul-20ul, preferably 2ul-10ul, and the dosage of the blood sample 4 is 20ul-60ul.

Preferably, during the movement of the pressure pump 3, the relative position of the blood sample 4 and the pressure main pipe 1 does not change; the movement time of the pressure pump 3 in one cycle is 0.5s～10s, preferably 2s～10s, the pressure pump 3 moves to the pressure The dwell time at the smallest volume of cavity 5 is one sixth of a cycle.

According to another aspect of the present invention, a blood viscoelasticity detection method is provided, comprising a blood viscoelasticity detection device, in each movement cycle of the pressure pump 3, the starting point of the movement of the pressure pump 3 in the pressure branch pipe 2, The end point remains unchanged; the pressure pump 3 moves periodically along the axial direction of the pressure branch pipe 2; the pressure sensor 6 measures the pressure value of the pressure chamber 5 in each movement cycle of the pressure pump 3, and the measured value is the smallest volume of the pressure chamber 5 The control system is connected with a signal processing system, and the signal processing system analyzes the pressure value measured by the pressure sensor 6 and draws a curve of pressure changing with time.

1 and 2 show the structure diagrams of the pressure pump 3 at different positions in a movement cycle. Correspondingly, when the pressure 3 moves to the point where the volume of the cavity in the pressure branch 2 decreases, the blood sample 4 is deformed accordingly.

Selection of experimental parameters:

(1) The maximum volume change of the pressure chamber 5 is 2ul～20ul (preferably 2ul～10ul, more preferably 3ul);

(2) The dosage of the blood sample 4 is 20ul-60ul (preferably 50ul: the more the blood sample 4 is, the thicker the blood thin layer is formed, and then the blood sample 4 under the same pressure, the smaller the deformation amount of the blood sample 4 is. , resulting in a smaller relative change in the gas pressure in the cavity, that is, as the volume of the blood sample 4 increases, the formation rate of the first blood clot in the blood sample 4 slows down; while the blood sample 4 has a smaller volume , that is, the thinner the blood thin layer formed, the more it can reflect the real blood coagulation situation, but the less blood coagulation speed is too fast and it is difficult to measure; Carry out the experiment, according to the experimental effect, determine that 50ul is preferred);

(3) The diameter of the pressure main pipe 1 is preferably 3 mm (when the amount of blood is constant, the thinner the pipe diameter, the easier it is to form a blood column; when the pipe diameter is thicker, it is easier to form a blood thin layer, and the blood thin layer is in the pipeline. Deformation is more likely to occur; by comparing the styles of the pressure main pipe with 11mm, 2mm, and 3mm diameters, the 3mm diameter is the best);

(4) The ratio of the radii of the pressure main pipe 1 to the pressure branch pipe 2 is 1 to 5.5 (the preferred value is 5. Since the force required by the blood sample 4 is small, when the ratio of the radii of the pressure main pipe 1 to the pressure branch pipe 2 is 5 , can increase the moving distance of the pressure pump 3 in the pressure branch pipe 2, and facilitate the adjustment of the pressure pump 3);

(5) When the pressure pump 3 moves, the volume change of the pressure chamber 5 does not exceed 10% (the preferred value is 5%);

(6) The movement time of one cycle of the pressure pump 3 is 0.5s to 10s (preferably 2s to 10s), and the dwell time of the pressure pump 3 moving to the minimum volume of the pressure chamber 5 is one sixth of a cycle. (The preferred period is 6s, so that while monitoring the coagulation process of the blood sample 4, the stay time of the pressure pump 3 moving to the minimum volume of the pressure chamber 5 is 1s, which is also convenient for the pressure sensor 6 to measure the pressure value there.)

To sum up, in the specific experiment, the following values can be selected: the maximum volume change of the pressure chamber 5 is 3ul, the amount of blood sample 4 is 50ul, the diameter of the pressure main pipe 1 is 3mm, and the ratio of the radius of the pressure main pipe 1 to the pressure branch pipe 2 is 5, the volume change of the pressure chamber 5 is 5%, and the movement time of one cycle of the pressure pump 3 is 6s.

Since the blood sample 4 is in the process of gradual coagulation, the viscoelasticity of the blood sample 4 will change with the passage of time; therefore, the pressure pump 3 is controlled to perform a reciprocating periodic motion, and the blood sample 4 will be deformed correspondingly, and at different times By maintaining the same movement position of the pressure pump 3 in each cycle, the volume reduction of the cavity in the pressure branch pipe 2 remains the same. It can reflect the change of viscoelasticity of blood sample 4; draw the relationship curve between pressure and time to facilitate the comparison of different data.

The experimental process is:

a: determine the experimental parameters, set according to the preferred parameter values (pipe diameter of pressure main pipe 1, pipe diameter of pressure branch pipe 2, blood sample 4 amount) and load blood sample 4 into the detection device;

b: The pressure pump 3 is periodically moved in the pressure branch pipe 2 through the control system; the pressure sensor 6 measures the pressure value of the pressure chamber 5 in each movement cycle of the pressure pump 3, and the measured value is the smallest volume of the pressure chamber 5 pressure value at

c: The pressure value measured by the pressure sensor 6 is monitored by the control system, and when the pressure value remains unchanged, the pressure pump 3 stops moving;

d: The signal processing system analyzes the pressure value measured by the pressure sensor 6, and draws a curve of the pressure changing with time;

e: By comparing multiple curves to reflect the viscoelasticity of the blood sample 4.

The above descriptions are only preferred embodiments of the present application, and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of this application shall be included within the protection scope of this application.

Claims

A blood viscoelasticity detection device, characterized in that it comprises a pressure main pipe (1) whose one end is communicated with the atmosphere and is an open end, and the other end of the pressure main pipe (1) is a closed end and is provided with a pressure branch pipe (2), The pressure branch pipe (2) is provided with a pressure pump (3) and forms a seal, the open end of the pressure main pipe (1) is provided with a blood sample (4) and forms a seal, the blood sample (4) and the A closed pressure chamber (5) is formed between the pressure pumps (3); a pressure sensor (6) is arranged in the pressure chamber (5).
The blood viscoelasticity detection device according to claim 1, characterized in that, the axial direction of the pressure main pipe (1) is in the horizontal direction.
The blood viscoelasticity detection device according to claim 1, characterized in that, the ratio of the radius of the pressure main pipe (1) to the pressure branch pipe (2) is 1-5.5.
The blood viscoelasticity detection device according to claim 1, characterized in that, both the pressure main pipe (1) and the pressure branch pipe (2) are provided with a constant temperature device.
The blood viscoelasticity detection device according to claim 1, characterized in that a detection cavity (21) is protruded from the pressure branch pipe (2), and the end of the detection cavity (21) is connected to the pressure sensor (21). 6) Secure the connection and form a seal.
The blood viscoelasticity detection device according to claim 1, wherein the pressure pump (3) is connected with a control system, and the pressure pump (3) runs along the pressure branch pipe under the control of the control system. (2) Reciprocating periodic motion in the axial direction.
The blood viscoelasticity detection device according to claim 1, characterized in that, when the pressure pump (3) moves, the volume change of the pressure chamber (5) does not exceed 20%.
The blood viscoelasticity detection device according to claim 7, wherein the maximum volume change of the pressure chamber (5) is 2ul-20ul, and the dosage of the blood sample (4) is 20ul-60ul.
The blood viscoelasticity detection device according to claim 6, characterized in that, during the movement of the pressure pump (3), the relative positions of the blood sample (4) and the pressure main pipe (1) are different from each other. The movement time of one cycle of the pressure pump (3) is 0.5s-10s, and the residence time of the pressure pump (3) moving to the minimum volume of the pressure chamber (5) is one sixth of a cycle.
A method for detecting blood viscoelasticity, comprising the device for detecting blood viscoelasticity according to any one of claims 1 to 9, characterized in that, in each movement cycle of the pressure pump (3), the pressure The starting point and the end point of the movement of the pump (3) in the pressure branch pipe (2) remain unchanged; the pressure pump (3) performs periodic motion on the pressure branch pipe (2); the pressure sensor (6) The pressure value of the pressure chamber (5) in each movement cycle of the pressure pump (3) is measured, and the measured value is the pressure value at the minimum volume of the pressure chamber (5); the control system is connected with a signal A processing system, the signal processing system analyzes the pressure value measured by the pressure sensor (6), and draws a curve of pressure changing with time.