WO2022126845A1 - Blood viscoelasticity detection device and detection method - Google Patents
Blood viscoelasticity detection device and detection method Download PDFInfo
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- WO2022126845A1 WO2022126845A1 PCT/CN2021/074701 CN2021074701W WO2022126845A1 WO 2022126845 A1 WO2022126845 A1 WO 2022126845A1 CN 2021074701 W CN2021074701 W CN 2021074701W WO 2022126845 A1 WO2022126845 A1 WO 2022126845A1
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- 239000008280 blood Substances 0.000 title claims abstract description 112
- 210000004369 blood Anatomy 0.000 title claims abstract description 112
- 238000001514 detection method Methods 0.000 title claims abstract description 41
- 230000008859 change Effects 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 12
- 230000000737 periodic effect Effects 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 8
- 238000006073 displacement reaction Methods 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 description 18
- 230000008569 process Effects 0.000 description 9
- 230000015271 coagulation Effects 0.000 description 6
- 238000005345 coagulation Methods 0.000 description 6
- 208000007536 Thrombosis Diseases 0.000 description 4
- 230000023555 blood coagulation Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000013169 thromboelastometry Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N19/00—Investigating materials by mechanical methods
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
Definitions
- the invention relates to the field of viscoelasticity analysis, in particular to a blood viscoelasticity detection device and a detection method.
- Thromboelastometry technology is to study the coagulation process by continuously monitoring the changes of blood viscoelasticity during blood coagulation.
- the blood coagulation process and viscoelastic changes are reflected by measuring the firmness of blood clots, as shown in FIG. , the end of the metal shaft 106 is fixed with a pin 103 and the pin 103 is inserted into the blood sample 101, the metal shaft 106 is periodically rotated around its vertical axis by the sensitive spring 108; the movement of the pin 103 can detect the firmness of the blood clot, and also The optical detection device 105 can be continuously recorded by beam deflection, and the blood clot firmness versus time can be plotted.
- the structure of this kind of device is too complicated; the processing of the pin 103 and the metal shaft 106 is difficult and the cost is high, and the whole device must work in a stable working environment.
- the purpose of the present invention is to provide a blood viscoelasticity detection device and a detection method with a simple structure and convenient measurement, aiming at the complex structure of the blood viscoelasticity detection device in the prior art.
- a blood viscoelasticity detection device comprising a pressure main pipe whose one end is communicated with the atmosphere and is an open end, the other end of the pressure main pipe is a closed end and is provided with a pressure branch pipe, the pressure branch pipe is provided with a pressure pump and forms a seal, and the open end of the pressure main pipe is The end is provided with a blood sample and forms a seal, and a closed pressure chamber is formed between the blood sample and the pressure pump; a pressure sensor is arranged in the pressure chamber.
- the fluid will produce corresponding deformation, and the elasticity of the fluid can be represented by the deformation value; and this device uses the principle of fluid mechanics, through the pressure
- a pressure pump is installed in the branch pipe, and the air in the pressure chamber is compressed by the movement of the pressure pump, and then a uniform force is applied to the blood sample through the air, so that the blood sample is deformed, and at the same time, it is ensured that the force does not push the blood sample. ;
- the pressure pump is used to exert a force on the blood sample.
- the deformation of different blood samples is also different, which makes the pressure chamber The volume is different, so the pressure value in the pressure chamber is different.
- the device separates the pressure main pipe and the pressure branch pipe to distinguish the area where the pressure pump and the blood sample are located, which facilitates the pressure pump to provide a small force to ensure that the blood sample does not move in the pressure main pipe.
- the axial direction of the pressure main pipe is in the horizontal direction.
- the pressure main tube can be set in the vertical or horizontal direction, as long as the blood sample does not move in the pressure main tube; here, the pressure main tube is set horizontally to prevent the blood sample from being affected by its own gravity. Stress governs sports conditions.
- the ratio of the radius of the pressure main pipe and the pressure branch pipe is 1-5.5.
- a constant temperature device is provided on both the pressure main pipe and the pressure branch pipe.
- the constant temperature device ensures that the temperature of the blood sample and the pressure chamber is consistent throughout the measurement process, reducing variables and improving the accuracy of the test results.
- a detection cavity is provided on the pressure branch pipe, and the end of the detection cavity is fixedly connected with the pressure sensor to form a seal.
- Reasonably set the position of the detection chamber so that the blood sample can be loaded into the pressure main tube before the experiment starts; protect the integrity of the loaded blood sample and avoid the blood sample sticking to the pressure sensor, thus affecting the detection result.
- the pressure pump is connected with a control system, and the pressure pump performs periodic reciprocating movement along the axial direction of the pressure branch pipe under the control of the control system. Since the blood sample is in the process of gradual coagulation, the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. , to reflect the change in viscoelasticity of blood samples over time.
- the volume change of the pressure chamber does not exceed 20%.
- the maximum change in the volume of the pressure chamber is 2ul-20ul, and the dosage of the blood sample is 20ul-60ul.
- the maximum change in the volume of the pressure chamber is set corresponding to the amount of the blood sample, which avoids the movement of the blood sample in the pressure main tube and facilitates the movement of the pressure pump.
- the relative position of the blood sample and the pressure main pipe does not change; the movement time of one cycle of the pressure pump is 0.5s to 10s, and the stay time of the pressure pump moving to the minimum volume of the pressure chamber is six minutes. one cycle.
- a method for detecting blood viscoelasticity including a blood viscoelasticity detecting device.
- the pressure pump performs periodic movement of the pressure branch pipe;
- the pressure sensor measures the pressure value of the pressure chamber in each movement cycle of the pressure pump, and the measured value is the pressure value at the minimum volume of the pressure chamber;
- the control system is connected with a signal processing system , the signal processing system analyzes the pressure value measured by the pressure sensor and draws a curve of the pressure changing with time.
- the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. ; By maintaining the same movement position of the pressure pump in each cycle, the volume reduction of the cavity in the pressure branch pipe remains the same. Changes in the viscoelasticity of blood samples; draw the relationship curve between pressure and time to facilitate the comparison of different data to qualitatively analyze the viscoelasticity of blood samples.
- a pressure pump is set in the pressure branch pipe, and the pressure is adjusted by the movement of the pressure pump.
- the air in the cavity is compressed, and then a uniform force is applied to the blood sample through the air, so that the blood sample is deformed, and at the same time, the force does not push the blood sample to move;
- the deformation amount of different blood samples is also different, so that the volume of the pressure chamber is different, and the pressure value in the pressure chamber is different.
- the measurement and comparison of the pressure value in the pressure chamber can characterize the state of the elastic modulus of the blood sample.
- the separate pressure main pipe and pressure branch pipe of the device distinguish the area where the pressure pump and the blood sample are located, which facilitates the pressure pump to provide a small force to ensure that the blood sample does not move in the pressure main pipe;
- the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. ; By maintaining the same movement position of the pressure pump in each cycle, the volume reduction of the cavity in the pressure branch pipe remains the same. Changes in the viscoelasticity of blood samples; draw the relationship curve between pressure and time to facilitate the comparison of different data to qualitatively analyze the viscoelasticity of blood samples.
- FIG. 1 shows the structure diagram of the blood viscoelasticity detection device in the prior art of the present invention.
- FIG. 2 shows the structure diagram of the pressure pump in the first position of a blood viscoelasticity detection device and detection method of the present invention.
- FIG. 3 shows the structure diagram of the pressure pump in the second position of a blood viscoelasticity detection device and detection method of the present invention.
- a blood viscoelasticity detection device includes a pressure main pipe 1 whose one end is connected to the atmosphere and is an open end, and the other end of the pressure main pipe 1 is a closed end and is provided with a pressure branch pipe 2.
- the pressure branch pipe 2 is provided with There is an axially movable pressure pump 3 and forms a seal, the open end of the pressure main pipe 1 is provided with a blood sample 4 and forms a seal, and a closed pressure chamber 5 is formed between the blood sample 4 and the pressure pump 3; pressure sensor 6.
- the axial direction of the pressure main pipe 1 is in the horizontal direction.
- the ratio of the radius of the pressure main pipe 1 to the pressure branch pipe 2 is 1-5.5.
- a constant temperature device is provided on both the pressure main pipe 1 and the pressure branch pipe 2 .
- a detection cavity 21 is protruded from the pressure branch pipe 2, and the end of the detection cavity 21 is fixedly connected with the pressure sensor 6 to form a seal.
- the pressure pump 3 is connected with a control system, and under the control of the control system, the pressure pump 3 performs a reciprocating periodic movement along the axial direction of the pressure branch pipe 2 .
- the volume change of the pressure chamber 5 is not more than 20%, preferably not more than 10%.
- the maximum volume change of the pressure chamber 5 is 2ul-20ul, preferably 2ul-10ul, and the dosage of the blood sample 4 is 20ul-60ul.
- the relative position of the blood sample 4 and the pressure main pipe 1 does not change; the movement time of the pressure pump 3 in one cycle is 0.5s ⁇ 10s, preferably 2s ⁇ 10s, the pressure pump 3 moves to the pressure
- the dwell time at the smallest volume of cavity 5 is one sixth of a cycle.
- a blood viscoelasticity detection method comprising a blood viscoelasticity detection device, in each movement cycle of the pressure pump 3, the starting point of the movement of the pressure pump 3 in the pressure branch pipe 2, The end point remains unchanged; the pressure pump 3 moves periodically along the axial direction of the pressure branch pipe 2; the pressure sensor 6 measures the pressure value of the pressure chamber 5 in each movement cycle of the pressure pump 3, and the measured value is the smallest volume of the pressure chamber 5
- the control system is connected with a signal processing system, and the signal processing system analyzes the pressure value measured by the pressure sensor 6 and draws a curve of pressure changing with time.
- FIG. 1 and 2 show the structure diagrams of the pressure pump 3 at different positions in a movement cycle.
- the pressure 3 moves to the point where the volume of the cavity in the pressure branch 2 decreases, the blood sample 4 is deformed accordingly.
- the maximum volume change of the pressure chamber 5 is 2ul ⁇ 20ul (preferably 2ul ⁇ 10ul, more preferably 3ul);
- the dosage of the blood sample 4 is 20ul-60ul (preferably 50ul: the more the blood sample 4 is, the thicker the blood thin layer is formed, and then the blood sample 4 under the same pressure, the smaller the deformation amount of the blood sample 4 is. , resulting in a smaller relative change in the gas pressure in the cavity, that is, as the volume of the blood sample 4 increases, the formation rate of the first blood clot in the blood sample 4 slows down; while the blood sample 4 has a smaller volume , that is, the thinner the blood thin layer formed, the more it can reflect the real blood coagulation situation, but the less blood coagulation speed is too fast and it is difficult to measure; Carry out the experiment, according to the experimental effect, determine that 50ul is preferred);
- the diameter of the pressure main pipe 1 is preferably 3 mm (when the amount of blood is constant, the thinner the pipe diameter, the easier it is to form a blood column; when the pipe diameter is thicker, it is easier to form a blood thin layer, and the blood thin layer is in the pipeline. Deformation is more likely to occur; by comparing the styles of the pressure main pipe with 11mm, 2mm, and 3mm diameters, the 3mm diameter is the best);
- the ratio of the radii of the pressure main pipe 1 to the pressure branch pipe 2 is 1 to 5.5 (the preferred value is 5. Since the force required by the blood sample 4 is small, when the ratio of the radii of the pressure main pipe 1 to the pressure branch pipe 2 is 5 , can increase the moving distance of the pressure pump 3 in the pressure branch pipe 2, and facilitate the adjustment of the pressure pump 3);
- the movement time of one cycle of the pressure pump 3 is 0.5s to 10s (preferably 2s to 10s), and the dwell time of the pressure pump 3 moving to the minimum volume of the pressure chamber 5 is one sixth of a cycle.
- the preferred period is 6s, so that while monitoring the coagulation process of the blood sample 4, the stay time of the pressure pump 3 moving to the minimum volume of the pressure chamber 5 is 1s, which is also convenient for the pressure sensor 6 to measure the pressure value there.
- the following values can be selected: the maximum volume change of the pressure chamber 5 is 3ul, the amount of blood sample 4 is 50ul, the diameter of the pressure main pipe 1 is 3mm, and the ratio of the radius of the pressure main pipe 1 to the pressure branch pipe 2 is 5, the volume change of the pressure chamber 5 is 5%, and the movement time of one cycle of the pressure pump 3 is 6s.
- the viscoelasticity of the blood sample 4 will change with the passage of time; therefore, the pressure pump 3 is controlled to perform a reciprocating periodic motion, and the blood sample 4 will be deformed correspondingly, and at different times
- the volume reduction of the cavity in the pressure branch pipe 2 remains the same. It can reflect the change of viscoelasticity of blood sample 4; draw the relationship curve between pressure and time to facilitate the comparison of different data.
- a determine the experimental parameters, set according to the preferred parameter values (pipe diameter of pressure main pipe 1, pipe diameter of pressure branch pipe 2, blood sample 4 amount) and load blood sample 4 into the detection device;
- the pressure pump 3 is periodically moved in the pressure branch pipe 2 through the control system; the pressure sensor 6 measures the pressure value of the pressure chamber 5 in each movement cycle of the pressure pump 3, and the measured value is the smallest volume of the pressure chamber 5 pressure value at
- the signal processing system analyzes the pressure value measured by the pressure sensor 6, and draws a curve of the pressure changing with time;
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Abstract
A blood viscoelasticity detection device and detection method. The detection device comprises a pressure main pipe (1) one end of which communicates with the atmosphere and is an open end. The other end of the pressure main pipe (1) is a closed end and is branched with a pressure branch pipe (2). The pressure branch pipe (2) is provided with an axially movable pressure pump (3) and forms a seal. The open end of the pressure main pipe (1) is provided with a blood sample (4) and forms a seal. A closed pressure chamber (5) is formed between the blood sample (4) and the pressure pump (3). A pressure sensor (6) is provided in the pressure chamber (5). The detection method is implemented on the basis of the aforementioned device. The pressure pump (3) exerts an action force on the blood sample (4) during detection. When the displacement of the pressure pump (3) is the same, due to the differences in shear stress of different blood samples (4), the deformation amounts of different blood samples (4) are also different, so that the volume of the pressure chamber (5) is different, and thus pressure values in the pressure chamber (5) are different. By means of measuring and comparing the pressure values in the pressure chamber (5), the states of the elastic moduli of the blood samples (4) may be characterized. Finally, the relationship between pressure and time is plotted to facilitate the comparison of different viscoelasticities.
Description
本发明涉及粘弹性分析领域,特别涉及一种血液粘弹性的检测装置及检测方法。The invention relates to the field of viscoelasticity analysis, in particular to a blood viscoelasticity detection device and a detection method.
血栓弹性测量技术,是通过对血液凝固过程中血液粘弹性变化的连续监测来研究凝固过程。现有装置中,通过对血凝块牢固度的量度,以反映血液的凝固过程及粘弹性变化,如图1所示,具体为:金属轴106通过由滚珠轴承107可旋转地固定到底板上,金属轴106端部固定有销103且销103插入血液样本101中,金属轴106通过敏感弹簧108围绕其垂直轴线周期性地旋转;销103的运动,可以检测血凝块的牢固度,也能够通过光学检测装置105,通过光束偏转来连续记录,并且能够绘制出血凝块牢固度与时间的关系曲线。但是该种装置,结构过于复杂;销103、金属轴106的加工难度大,成本高,且整个装置必须在稳定的工作环境下工作。Thromboelastometry technology is to study the coagulation process by continuously monitoring the changes of blood viscoelasticity during blood coagulation. In the existing device, the blood coagulation process and viscoelastic changes are reflected by measuring the firmness of blood clots, as shown in FIG. , the end of the metal shaft 106 is fixed with a pin 103 and the pin 103 is inserted into the blood sample 101, the metal shaft 106 is periodically rotated around its vertical axis by the sensitive spring 108; the movement of the pin 103 can detect the firmness of the blood clot, and also The optical detection device 105 can be continuously recorded by beam deflection, and the blood clot firmness versus time can be plotted. However, the structure of this kind of device is too complicated; the processing of the pin 103 and the metal shaft 106 is difficult and the cost is high, and the whole device must work in a stable working environment.
发明内容SUMMARY OF THE INVENTION
本发明的目的在于:针对现有技术中血液粘弹性检测装置的结构复杂的问题,提供一种结构简单,测量方便的血液粘弹性的检测装置及检测方法。The purpose of the present invention is to provide a blood viscoelasticity detection device and a detection method with a simple structure and convenient measurement, aiming at the complex structure of the blood viscoelasticity detection device in the prior art.
为了实现上述发明目的,根据本发明的一个方面,提供了以下技术方案:In order to achieve the above-mentioned purpose of the invention, according to one aspect of the present invention, the following technical solutions are provided:
一种血液粘弹性的检测装置,包括一端与大气连通且为开放端的压力主管,压力主管的另一端为封闭端并支出有压力支管,压力支管上设有压力泵并形成密封,压力主管的开放端设有血液样本并形成密封,血液样本和压力泵间形成 一个封闭的压力腔;压力腔内设有压力传感器。A blood viscoelasticity detection device, comprising a pressure main pipe whose one end is communicated with the atmosphere and is an open end, the other end of the pressure main pipe is a closed end and is provided with a pressure branch pipe, the pressure branch pipe is provided with a pressure pump and forms a seal, and the open end of the pressure main pipe is The end is provided with a blood sample and forms a seal, and a closed pressure chamber is formed between the blood sample and the pressure pump; a pressure sensor is arranged in the pressure chamber.
采用上述技术方案的本发明,根据流体力学原理,流体在管道中流动时,流体内部的剪切应力会阻止流体流动,在低速流下,流体表现为弹性的特点;如果在静止流体的一侧施加作用力,保证该作用力不使流体移动或流速尽可能接近为零,此时流体会产生相应的形变,通过该形变值可以表征流体的弹性大小;而本装置利用流体力学原理,通过在压力支管内设置压力泵,通过压力泵的移动来对压力腔内的空气进行压缩,进而通过空气给血液样本施加一个均匀的作用力,使得血液样本产生形变,同时保证该作用力不推动血液样本运动;此外,进行检测时,通过压力泵对血液样本施加作用力,在压力泵相同位移的情况下,由于不同血液样本自身剪切应力不同,因而不同血液样本的形变量也不同,使得压力腔的体积不同,从而压力腔内的压力值不同,通过对压力腔内压力值的测定并进行对比,可以表征血液样本的弹性模量的状况。该装置分设的压力主管和压力支管,将压力泵和血液样本所在的区域进行了区分,方便了压力泵提供较小的作用力,以保证血液样本不在压力主管内移动。In the present invention adopting the above technical solutions, according to the principle of fluid mechanics, when the fluid flows in the pipeline, the shear stress inside the fluid will prevent the fluid from flowing, and under low-speed flow, the fluid exhibits elastic characteristics; Acting force to ensure that the acting force does not make the fluid move or the flow rate is as close to zero as possible. At this time, the fluid will produce corresponding deformation, and the elasticity of the fluid can be represented by the deformation value; and this device uses the principle of fluid mechanics, through the pressure A pressure pump is installed in the branch pipe, and the air in the pressure chamber is compressed by the movement of the pressure pump, and then a uniform force is applied to the blood sample through the air, so that the blood sample is deformed, and at the same time, it is ensured that the force does not push the blood sample. ; In addition, when testing, the pressure pump is used to exert a force on the blood sample. In the case of the same displacement of the pressure pump, due to the different shear stress of different blood samples, the deformation of different blood samples is also different, which makes the pressure chamber The volume is different, so the pressure value in the pressure chamber is different. By measuring and comparing the pressure value in the pressure chamber, the state of the elastic modulus of the blood sample can be characterized. The device separates the pressure main pipe and the pressure branch pipe to distinguish the area where the pressure pump and the blood sample are located, which facilitates the pressure pump to provide a small force to ensure that the blood sample does not move in the pressure main pipe.
进一步地,压力主管的轴向在水平方向。压力主管可以设置在竖直方向或水平方向,只要保证血液样本不在压力主管内进行移动既可;此处将压力主管水平设置,避免了血液样本由于受到自身重力的影响,从而在竖直方向沿压力主管运动的状况。Further, the axial direction of the pressure main pipe is in the horizontal direction. The pressure main tube can be set in the vertical or horizontal direction, as long as the blood sample does not move in the pressure main tube; here, the pressure main tube is set horizontally to prevent the blood sample from being affected by its own gravity. Stress governs sports conditions.
进一步地,压力主管和压力支管的半径之比为1~5.5。合理设置压力主管和压力支管半径的相对值,方便对血液样本受到的作用力进行微调。Further, the ratio of the radius of the pressure main pipe and the pressure branch pipe is 1-5.5. Reasonably set the relative value of the radius of the pressure main pipe and the pressure branch pipe to facilitate fine-tuning of the force on the blood sample.
进一步地,压力主管和压力支管上均设有恒温装置。恒温装置保证血液样本、压力腔在整个测量过程中的温度一致,减小变量,提高检测结果的精准度。Further, a constant temperature device is provided on both the pressure main pipe and the pressure branch pipe. The constant temperature device ensures that the temperature of the blood sample and the pressure chamber is consistent throughout the measurement process, reducing variables and improving the accuracy of the test results.
进一步地,压力支管上支出有一个检测腔,检测腔的末端与压力传感器固定连接并形成密封。合理设置检测腔的位置,方便实验开始前,血液样本装入压力主管;保护装入血液样本量的完整性,避免出现血液样本粘附在压力传感器上,从而影响检测结果的状况。Further, a detection cavity is provided on the pressure branch pipe, and the end of the detection cavity is fixedly connected with the pressure sensor to form a seal. Reasonably set the position of the detection chamber so that the blood sample can be loaded into the pressure main tube before the experiment starts; protect the integrity of the loaded blood sample and avoid the blood sample sticking to the pressure sensor, thus affecting the detection result.
进一步地,压力泵连接有控制系统,压力泵在控制系统的控制下沿压力支管轴向做往复的周期性运。由于血液样本处于逐步凝固的过程,随着时间的推移,血液样本的粘弹性将产生变化;因而控制压力泵做往复的周期性运动,血液样本将产生对应的形变,且不同时间的形变量不同,以反映血液样本粘弹性随时间的变化。Further, the pressure pump is connected with a control system, and the pressure pump performs periodic reciprocating movement along the axial direction of the pressure branch pipe under the control of the control system. Since the blood sample is in the process of gradual coagulation, the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. , to reflect the change in viscoelasticity of blood samples over time.
进一步地,在压力泵运动时,压力腔的体积变化量不超过20%。合理设置压力腔体积变化量,以合理设置压力泵的运动范围,进而合理设置血液样本受到的作用力,避免血液样本在压力主管内移动的同时,方便测量与压力泵位置的调节。Further, when the pressure pump moves, the volume change of the pressure chamber does not exceed 20%. Reasonably set the volume change of the pressure chamber to reasonably set the movement range of the pressure pump, and then set the force on the blood sample reasonably, to avoid the blood sample moving in the pressure main tube, and to facilitate the measurement and the adjustment of the pressure pump position.
进一步地,压力腔体积的最大变化量为2ul~20ul,血液样本的用量为20ul~60ul。压力腔体积的最大变化量与血液样本的用量对应设置,避免血液样本在压力主管内移动的同时,方便压力泵的运动。Further, the maximum change in the volume of the pressure chamber is 2ul-20ul, and the dosage of the blood sample is 20ul-60ul. The maximum change in the volume of the pressure chamber is set corresponding to the amount of the blood sample, which avoids the movement of the blood sample in the pressure main tube and facilitates the movement of the pressure pump.
进一步地,在压力泵的运动过程中,血液样本与压力主管的相对位置不变;压力泵一个周期的运动时间为0.5s~10s,压力泵运动至压力腔体积最小处的停留时长为六分之一个周期。合理设置压力泵的运动周期,并使得压力泵在压力腔体积最小处进行适当的停留,以方便压力传感器对压力腔内气压的测量。Further, during the movement of the pressure pump, the relative position of the blood sample and the pressure main pipe does not change; the movement time of one cycle of the pressure pump is 0.5s to 10s, and the stay time of the pressure pump moving to the minimum volume of the pressure chamber is six minutes. one cycle. Reasonably set the movement period of the pressure pump, and make the pressure pump stop at the minimum volume of the pressure chamber, so as to facilitate the pressure sensor to measure the air pressure in the pressure chamber.
根据本发明的另一方面,提供了一种血液粘弹性的检测方法,包含血液粘弹性的检测装置,在压力泵的每个运动周期中,压力泵在压力支管中运动的起 点、终点保持不变;压力泵对压力支管进行周期性运动;压力传感器对压力泵每个运动周期内压力腔的压力值进行测量,且测量值为压力腔体积最小处的压力值;控制系统连接有信号处理系统,信号处理系统对压力传感器测得的压力值进行分析,并绘制一条压力随时间变化的曲线。由于血液样本处于逐步凝固的过程,随着时间的推移,血液样本的粘弹性将产生变化;因而控制压力泵做往复的周期性运动,血液样本将产生对应的形变,且不同时间的形变量不同;通过维持的压力泵每个周期的运动位置一致,使得压力支管内空腔的体积减小量保持相同,通过使压力泵连续运动,同时对压力腔体积最小处的压力值进行测量,可以反映血液样本粘弹性的变化;绘制压力、时间的关系曲线,方便不同数据的比对,以对血液样本的粘弹性进行定性的分析。According to another aspect of the present invention, a method for detecting blood viscoelasticity is provided, including a blood viscoelasticity detecting device. In each movement cycle of the pressure pump, the starting point and the end point of the pressure pump moving in the pressure branch remain unchanged. The pressure pump performs periodic movement of the pressure branch pipe; the pressure sensor measures the pressure value of the pressure chamber in each movement cycle of the pressure pump, and the measured value is the pressure value at the minimum volume of the pressure chamber; the control system is connected with a signal processing system , the signal processing system analyzes the pressure value measured by the pressure sensor and draws a curve of the pressure changing with time. Since the blood sample is in the process of gradual coagulation, the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. ; By maintaining the same movement position of the pressure pump in each cycle, the volume reduction of the cavity in the pressure branch pipe remains the same. Changes in the viscoelasticity of blood samples; draw the relationship curve between pressure and time to facilitate the comparison of different data to qualitatively analyze the viscoelasticity of blood samples.
与现有技术相比,本发明的有益效果:Compared with the prior art, the beneficial effects of the present invention:
根据流体力学,流体在管道中流动时,流体内部的剪切应力会阻止流体流动,在低速流下,流体表现为弹性的特点;如果在静止流体的一侧施加作用力,保证该作用力不使流体移动或流速尽可能接近为零,此时流体会产生相应的形变,通过该形变值可以表征流体的弹性大小;而本装置通过在压力支管内设置压力泵,通过压力泵的移动来对压力腔内的空气进行压缩,进而通过空气给血液样本施加一个均匀的作用力,使得血液样本产生形变,同时保证该作用力不推动血液样本运动;此外,进行检测时,通过压力泵对血液样本施加作用力,在压力泵相同位移的情况下,由于不同血液样本自身剪切应力不同,因而不同血液样本的形变量也不同,使得压力腔的体积不同,从而压力腔内的压力值不同,通过对压力腔内压力值的测定并进行对比,可以表征血液样本的弹性模量的状况。该装置分设的压力主管和压力支管,将压力泵和血液样本所在的区域 进行了区分,方便了压力泵提供较小的作用力,以保证血液样本不在压力主管内移动;According to fluid mechanics, when a fluid flows in a pipe, the shear stress inside the fluid will prevent the fluid from flowing. Under low-speed flow, the fluid exhibits elastic characteristics; if a force is applied on one side of the static fluid, ensure that the force does not cause When the fluid moves or the flow rate is as close to zero as possible, the fluid will produce corresponding deformation, and the elasticity of the fluid can be represented by the deformation value. In this device, a pressure pump is set in the pressure branch pipe, and the pressure is adjusted by the movement of the pressure pump. The air in the cavity is compressed, and then a uniform force is applied to the blood sample through the air, so that the blood sample is deformed, and at the same time, the force does not push the blood sample to move; In the case of the same displacement of the pressure pump, due to the different shear stress of different blood samples, the deformation amount of different blood samples is also different, so that the volume of the pressure chamber is different, and the pressure value in the pressure chamber is different. The measurement and comparison of the pressure value in the pressure chamber can characterize the state of the elastic modulus of the blood sample. The separate pressure main pipe and pressure branch pipe of the device distinguish the area where the pressure pump and the blood sample are located, which facilitates the pressure pump to provide a small force to ensure that the blood sample does not move in the pressure main pipe;
由于血液样本处于逐步凝固的过程,随着时间的推移,血液样本的粘弹性将产生变化;因而控制压力泵做往复的周期性运动,血液样本将产生对应的形变,且不同时间的形变量不同;通过维持的压力泵每个周期的运动位置一致,使得压力支管内空腔的体积减小量保持相同,通过使压力泵连续运动,同时对压力腔体积最小处的压力值进行测量,可以反映血液样本粘弹性的变化;绘制压力、时间的关系曲线,方便不同数据的比对,以对血液样本的粘弹性进行定性的分析。Since the blood sample is in the process of gradual coagulation, the viscoelasticity of the blood sample will change with the passage of time; therefore, the blood sample will be deformed correspondingly by controlling the pressure pump to perform reciprocating periodic motion, and the deformation amount at different times is different. ; By maintaining the same movement position of the pressure pump in each cycle, the volume reduction of the cavity in the pressure branch pipe remains the same. Changes in the viscoelasticity of blood samples; draw the relationship curve between pressure and time to facilitate the comparison of different data to qualitatively analyze the viscoelasticity of blood samples.
构成本申请的一部分的说明书附图用来提供对发明的进一步理解,本实用新型的示意性实施例及其说明用于解释本实用新型,并不构成对本实用新型的不当限定。在附图中:The accompanying drawings forming a part of the present application are used to provide further understanding of the invention, and the schematic embodiments and descriptions of the present invention are used to explain the present invention and do not constitute an improper limitation of the present invention. In the attached image:
图1示出本发明的现有技术中血液粘弹性检测装置的结构图。FIG. 1 shows the structure diagram of the blood viscoelasticity detection device in the prior art of the present invention.
图2示出了本发明一种血液粘弹性的检测装置及检测方法的压力泵在第一个位置的结构图。FIG. 2 shows the structure diagram of the pressure pump in the first position of a blood viscoelasticity detection device and detection method of the present invention.
图3示出了本发明一种血液粘弹性的检测装置及检测方法的压力泵在第二个位置的结构图。FIG. 3 shows the structure diagram of the pressure pump in the second position of a blood viscoelasticity detection device and detection method of the present invention.
其中,上述附图包括以下附图标记:Wherein, the above-mentioned drawings include the following reference signs:
1压力主管;1 pressure supervisor;
2压力支管;21检测腔;2 pressure branch pipes; 21 detection chambers;
3压力泵;4血液样本;5压力腔;6压力传感器。3 pressure pumps; 4 blood samples; 5 pressure chambers; 6 pressure sensors.
需要指出的是,除非另有指明,本申请使用的所有技术和科学术语具有与本申请所属技术领域的普通技术人员通常理解的相同含义。It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
为了使本技术领域的人员更好地理解本申请方案,下面将结合本申请实施例中的附图,对本申请实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本申请一部分的实施例,而不是全部的实施例。基于本申请中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都应当属于本申请保护的范围。In order to make those skilled in the art better understand the solutions of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only The embodiments are part of the present application, but not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the scope of protection of the present application.
如图1和图2,一种血液粘弹性的检测装置,包括一端与大气连通且为开放端的压力主管1,压力主管1的另一端为封闭端并支出有压力支管2,压力支管2上设有轴向可移动的压力泵3并形成密封,压力主管1的开放端设有血液样本4并形成密封,血液样本4和压力泵3间形成一个封闭的压力腔5;压力腔5内设有压力传感器6。1 and 2, a blood viscoelasticity detection device includes a pressure main pipe 1 whose one end is connected to the atmosphere and is an open end, and the other end of the pressure main pipe 1 is a closed end and is provided with a pressure branch pipe 2. The pressure branch pipe 2 is provided with There is an axially movable pressure pump 3 and forms a seal, the open end of the pressure main pipe 1 is provided with a blood sample 4 and forms a seal, and a closed pressure chamber 5 is formed between the blood sample 4 and the pressure pump 3; pressure sensor 6.
优选的,压力主管1的轴向在水平方向。Preferably, the axial direction of the pressure main pipe 1 is in the horizontal direction.
优选的,压力主管1和压力支管2的半径之比为1~5.5。Preferably, the ratio of the radius of the pressure main pipe 1 to the pressure branch pipe 2 is 1-5.5.
优选的,压力主管1和压力支管2上均设有恒温装置。Preferably, a constant temperature device is provided on both the pressure main pipe 1 and the pressure branch pipe 2 .
优选的,压力支管2上支出有一个检测腔21,检测腔21的末端与压力传感器6固定连接并形成密封。Preferably, a detection cavity 21 is protruded from the pressure branch pipe 2, and the end of the detection cavity 21 is fixedly connected with the pressure sensor 6 to form a seal.
优选的,压力泵3连接有控制系统,压力泵3在控制系统的控制下沿压力支管2轴向做往复的周期性运动。Preferably, the pressure pump 3 is connected with a control system, and under the control of the control system, the pressure pump 3 performs a reciprocating periodic movement along the axial direction of the pressure branch pipe 2 .
优选的,在压力泵3运动时,压力腔5的体积变化量不超过20%,优选不 超过10%。Preferably, when the pressure pump 3 is in motion, the volume change of the pressure chamber 5 is not more than 20%, preferably not more than 10%.
优选的,压力腔5体积的最大变化量为2ul~20ul,优选2ul~10ul,血液样本4的用量为20ul~60ul。Preferably, the maximum volume change of the pressure chamber 5 is 2ul-20ul, preferably 2ul-10ul, and the dosage of the blood sample 4 is 20ul-60ul.
优选的,在压力泵3的运动过程中,血液样本4与压力主管1的相对位置不变;压力泵3一个周期的运动时间为0.5s~10s,优选2s~10s,压力泵3运动至压力腔5体积最小处的停留时长为六分之一个周期。Preferably, during the movement of the pressure pump 3, the relative position of the blood sample 4 and the pressure main pipe 1 does not change; the movement time of the pressure pump 3 in one cycle is 0.5s~10s, preferably 2s~10s, the pressure pump 3 moves to the pressure The dwell time at the smallest volume of cavity 5 is one sixth of a cycle.
根据本发明的另一方面,提供了一种血液粘弹性的检测方法,包含血液粘弹性的检测装置,在压力泵3的每个运动周期中,压力泵3在压力支管2中运动的起点、终点保持不变;压力泵3沿压力支管2轴向进行周期性运动;压力传感器6对压力泵3每个运动周期内压力腔5的压力值进行测量,且测量值为压力腔5体积最小处的压力值;控制系统连接有信号处理系统,信号处理系统对压力传感器6测得的压力值进行分析,并绘制一条压力随时间变化的曲线。According to another aspect of the present invention, a blood viscoelasticity detection method is provided, comprising a blood viscoelasticity detection device, in each movement cycle of the pressure pump 3, the starting point of the movement of the pressure pump 3 in the pressure branch pipe 2, The end point remains unchanged; the pressure pump 3 moves periodically along the axial direction of the pressure branch pipe 2; the pressure sensor 6 measures the pressure value of the pressure chamber 5 in each movement cycle of the pressure pump 3, and the measured value is the smallest volume of the pressure chamber 5 The control system is connected with a signal processing system, and the signal processing system analyzes the pressure value measured by the pressure sensor 6 and draws a curve of pressure changing with time.
图1和图2,示出了压力泵3在一个运动周期的不同位置的结构图,相应的,当压力3运动至压力支管2内腔体体积减小时,血液样本4产生了相应的形变。1 and 2 show the structure diagrams of the pressure pump 3 at different positions in a movement cycle. Correspondingly, when the pressure 3 moves to the point where the volume of the cavity in the pressure branch 2 decreases, the blood sample 4 is deformed accordingly.
实验参数的选定:Selection of experimental parameters:
(1)压力腔5体积的最大变化量为2ul~20ul(优选2ul~10ul,更优选为3ul);(1) The maximum volume change of the pressure chamber 5 is 2ul~20ul (preferably 2ul~10ul, more preferably 3ul);
(2)血液样本4的用量为20ul~60ul(优选50ul:血液样本4量越多,形成的血液薄层越厚,那么血液样本4在同等压强下,血液样本4发生的形变量就越小,进而导致空腔中气体压强的相对变化量就越小,即随着血液样本4体积的增大,血液样本4中第一块血凝块形成的速率减慢;而血液样本4量较少,即形成的血液薄层越薄,越能反应出血液真实的凝固情况,但较少的血液凝固 速度过快,不易测量;因而通过对20ul、35ul、50ul、70ul、90ul的血液样本4分别进行实验,根据实验效果确定50ul为优选);(2) The dosage of the blood sample 4 is 20ul-60ul (preferably 50ul: the more the blood sample 4 is, the thicker the blood thin layer is formed, and then the blood sample 4 under the same pressure, the smaller the deformation amount of the blood sample 4 is. , resulting in a smaller relative change in the gas pressure in the cavity, that is, as the volume of the blood sample 4 increases, the formation rate of the first blood clot in the blood sample 4 slows down; while the blood sample 4 has a smaller volume , that is, the thinner the blood thin layer formed, the more it can reflect the real blood coagulation situation, but the less blood coagulation speed is too fast and it is difficult to measure; Carry out the experiment, according to the experimental effect, determine that 50ul is preferred);
(3)压力主管1的直径优选3mm(当血液量一定时,管径越细,着血液更容易形成血柱;当管径较粗时,更容易形成血液薄层,血液薄层在管道中更容易发生形变;通过对压力主管11mm、2mm、3mm管径进行式样对比,3mm的管径效果最好);(3) The diameter of the pressure main pipe 1 is preferably 3 mm (when the amount of blood is constant, the thinner the pipe diameter, the easier it is to form a blood column; when the pipe diameter is thicker, it is easier to form a blood thin layer, and the blood thin layer is in the pipeline. Deformation is more likely to occur; by comparing the styles of the pressure main pipe with 11mm, 2mm, and 3mm diameters, the 3mm diameter is the best);
(4)压力主管1和压力支管2的半径之比为1~5.5(优选数值为5,由于血液样本4所需的作用力小,当压力主管1和压力支管2的半径之比为5时,可增大压力泵3在压力支管2中的移动距离,方便压力泵3的调节);(4) The ratio of the radii of the pressure main pipe 1 to the pressure branch pipe 2 is 1 to 5.5 (the preferred value is 5. Since the force required by the blood sample 4 is small, when the ratio of the radii of the pressure main pipe 1 to the pressure branch pipe 2 is 5 , can increase the moving distance of the pressure pump 3 in the pressure branch pipe 2, and facilitate the adjustment of the pressure pump 3);
(5)在压力泵3运动时,压力腔5的体积变化量不超过10%(优选数值为5%);(5) When the pressure pump 3 moves, the volume change of the pressure chamber 5 does not exceed 10% (the preferred value is 5%);
(6)压力泵3一个周期的运动时间为0.5s~10s(优选为2s~10s),压力泵3运动至压力腔5体积最小处的停留时长为六分之一个周期。(优选周期为6s,达到对血液样本4凝固过程进行监控的同时,压力泵3运动至压力腔5体积最小处的停留时长为1s,也方便压力传感器6对该处压力值的测定。)(6) The movement time of one cycle of the pressure pump 3 is 0.5s to 10s (preferably 2s to 10s), and the dwell time of the pressure pump 3 moving to the minimum volume of the pressure chamber 5 is one sixth of a cycle. (The preferred period is 6s, so that while monitoring the coagulation process of the blood sample 4, the stay time of the pressure pump 3 moving to the minimum volume of the pressure chamber 5 is 1s, which is also convenient for the pressure sensor 6 to measure the pressure value there.)
综上,具体实验时,可选用以下数值:压力腔5体积的最大变化量为3ul,血液样本4的用量为50ul,压力主管1的直径为3mm,压力主管1和压力支管2的半径之比为5,压力腔5的体积变化量为5%,压力泵3一个周期的运动时间为6s。To sum up, in the specific experiment, the following values can be selected: the maximum volume change of the pressure chamber 5 is 3ul, the amount of blood sample 4 is 50ul, the diameter of the pressure main pipe 1 is 3mm, and the ratio of the radius of the pressure main pipe 1 to the pressure branch pipe 2 is 5, the volume change of the pressure chamber 5 is 5%, and the movement time of one cycle of the pressure pump 3 is 6s.
由于血液样本4处于逐步凝固的过程,随着时间的推移,血液样本4的粘弹性将产生变化;因而控制压力泵3做往复的周期性运动,血液样本4将产生对应的形变,且不同时间的形变量不同;通过维持的压力泵3每个周期的运动位置一致,使得压力支管2内空腔的体积减小量保持相同,通过使压力泵3连 续运动,同时对压力腔5体积最小处的压力值进行测量,可以反映血液样本4粘弹性的变化;绘制压力、时间的关系曲线,方便不同数据的比对。Since the blood sample 4 is in the process of gradual coagulation, the viscoelasticity of the blood sample 4 will change with the passage of time; therefore, the pressure pump 3 is controlled to perform a reciprocating periodic motion, and the blood sample 4 will be deformed correspondingly, and at different times By maintaining the same movement position of the pressure pump 3 in each cycle, the volume reduction of the cavity in the pressure branch pipe 2 remains the same. It can reflect the change of viscoelasticity of blood sample 4; draw the relationship curve between pressure and time to facilitate the comparison of different data.
实验过程为:The experimental process is:
a:确定实验参数,根据优选的参数值(压力主管1管径、压力支管2管径、血液样本4量)进行设置并将血液样本4装入该检测装置;a: determine the experimental parameters, set according to the preferred parameter values (pipe diameter of pressure main pipe 1, pipe diameter of pressure branch pipe 2, blood sample 4 amount) and load blood sample 4 into the detection device;
b:通过控制系统使得压力泵3在压力支管2内做周期性的运动;压力传感器6对压力泵3每个运动周期内压力腔5的压力值进行测量,且测量值为压力腔5体积最小处的压力值;b: The pressure pump 3 is periodically moved in the pressure branch pipe 2 through the control system; the pressure sensor 6 measures the pressure value of the pressure chamber 5 in each movement cycle of the pressure pump 3, and the measured value is the smallest volume of the pressure chamber 5 pressure value at
c:通过控制系统对压力传感器6测的的压力值进行监测,当压力值维持不变时,压力泵3停止运动;c: The pressure value measured by the pressure sensor 6 is monitored by the control system, and when the pressure value remains unchanged, the pressure pump 3 stops moving;
d:信号处理系统对压力传感器6测得的压力值进行分析,并绘制一条压力随时间变化的曲线;d: The signal processing system analyzes the pressure value measured by the pressure sensor 6, and draws a curve of the pressure changing with time;
e:通过对多条曲线进行比对,以反映出血液样本4的粘弹性状况。e: By comparing multiple curves to reflect the viscoelasticity of the blood sample 4.
以上所述仅为本申请的优选实施例而已,并不用于限制本申请,对于本领域的技术人员来说,本申请可以有各种更改和变化。凡在本申请的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本申请的保护范围之内。The above descriptions are only preferred embodiments of the present application, and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of this application shall be included within the protection scope of this application.
Claims (10)
- 一种血液粘弹性的检测装置,其特征在于,包括一端与大气连通且为开放端的压力主管(1),所述压力主管(1)的另一端为封闭端并支出有压力支管(2),所述压力支管(2)上设有压力泵(3)并形成密封,所述压力主管(1)的开放端设有血液样本(4)并形成密封,所述血液样本(4)和所述压力泵(3)间形成一个封闭的压力腔(5);所述压力腔(5)内设有压力传感器(6)。A blood viscoelasticity detection device, characterized in that it comprises a pressure main pipe (1) whose one end is communicated with the atmosphere and is an open end, and the other end of the pressure main pipe (1) is a closed end and is provided with a pressure branch pipe (2), The pressure branch pipe (2) is provided with a pressure pump (3) and forms a seal, the open end of the pressure main pipe (1) is provided with a blood sample (4) and forms a seal, the blood sample (4) and the A closed pressure chamber (5) is formed between the pressure pumps (3); a pressure sensor (6) is arranged in the pressure chamber (5).
- 根据权利要求1所述的血液粘弹性的检测装置,其特征在于,所述压力主管(1)的轴向在水平方向。The blood viscoelasticity detection device according to claim 1, characterized in that, the axial direction of the pressure main pipe (1) is in the horizontal direction.
- 根据权利要求1所述的血液粘弹性的检测装置,其特征在于,所述压力主管(1)和所述压力支管(2)的半径之比为1~5.5。The blood viscoelasticity detection device according to claim 1, characterized in that, the ratio of the radius of the pressure main pipe (1) to the pressure branch pipe (2) is 1-5.5.
- 根据权利要求1所述的血液粘弹性的检测装置,其特征在于,所述压力主管(1)和所述压力支管(2)上均设有恒温装置。The blood viscoelasticity detection device according to claim 1, characterized in that, both the pressure main pipe (1) and the pressure branch pipe (2) are provided with a constant temperature device.
- 根据权利要求1所述的血液粘弹性的检测装置,其特征在于,所述压力支管(2)上支出有一个检测腔(21),所述检测腔(21)的末端与所述压力传感器(6)固定连接并形成密封。The blood viscoelasticity detection device according to claim 1, characterized in that a detection cavity (21) is protruded from the pressure branch pipe (2), and the end of the detection cavity (21) is connected to the pressure sensor (21). 6) Secure the connection and form a seal.
- 根据权利要求1所述的血液粘弹性的检测装置,其特征在于,所述压力泵(3)连接有控制系统,所述压力泵(3)在所述控制系统的控制下沿所述压力支管(2)轴向做往复的周期性运动。The blood viscoelasticity detection device according to claim 1, wherein the pressure pump (3) is connected with a control system, and the pressure pump (3) runs along the pressure branch pipe under the control of the control system. (2) Reciprocating periodic motion in the axial direction.
- 根据权利要求1所述的血液粘弹性的检测装置,其特征在于,在所述压力泵(3)运动时,所述压力腔(5)的体积变化量不超过20%。The blood viscoelasticity detection device according to claim 1, characterized in that, when the pressure pump (3) moves, the volume change of the pressure chamber (5) does not exceed 20%.
- 根据权利要求7所述的血液粘弹性的检测装置,其特征在于,所述压力腔(5)体积的最大变化量为2ul~20ul,所述血液样本(4)的用量为20ul~60ul。The blood viscoelasticity detection device according to claim 7, wherein the maximum volume change of the pressure chamber (5) is 2ul-20ul, and the dosage of the blood sample (4) is 20ul-60ul.
- 根据权利要求6所述的血液粘弹性的检测装置,其特征在于,在所述压力泵(3)的运动过程中,所述血液样本(4)与所述压力主管(1)的相对位置不变;所述压力泵(3)一个周期的运动时间为0.5s~10s,所述压力泵(3)运动至所述压力腔(5)体积最小处的停留时长为六分之一个周期。The blood viscoelasticity detection device according to claim 6, characterized in that, during the movement of the pressure pump (3), the relative positions of the blood sample (4) and the pressure main pipe (1) are different from each other. The movement time of one cycle of the pressure pump (3) is 0.5s-10s, and the residence time of the pressure pump (3) moving to the minimum volume of the pressure chamber (5) is one sixth of a cycle.
- 一种血液粘弹性的检测方法,包含权利要求1至9任一项所述的血液粘弹性的检测装置,其特征在于,在所述压力泵(3)的每个运动周期中,所述压力泵(3)在所述压力支管(2)中运动的起点、终点保持不变;所述压力泵(3)对所述压力支管(2)进行周期性运动;所述压力传感器(6)对所述压力泵(3)每个运动周期内所述压力腔(5)的压力值进行测量,且测量值为所述压力腔(5)体积最小处的压力值;所述控制系统连接有信号处理系统,所述信号处理系统对压力传感器(6)测得的压力值进行分析,并绘制一条压力随时间变化的曲线。A method for detecting blood viscoelasticity, comprising the device for detecting blood viscoelasticity according to any one of claims 1 to 9, characterized in that, in each movement cycle of the pressure pump (3), the pressure The starting point and the end point of the movement of the pump (3) in the pressure branch pipe (2) remain unchanged; the pressure pump (3) performs periodic motion on the pressure branch pipe (2); the pressure sensor (6) The pressure value of the pressure chamber (5) in each movement cycle of the pressure pump (3) is measured, and the measured value is the pressure value at the minimum volume of the pressure chamber (5); the control system is connected with a signal A processing system, the signal processing system analyzes the pressure value measured by the pressure sensor (6), and draws a curve of pressure changing with time.
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