WO2022120808A1

WO2022120808A1 - Antimicrobial use of aquilaria sinensis leaf extract and composition thereof

Info

Publication number: WO2022120808A1
Application number: PCT/CN2020/135778
Authority: WO
Inventors: 曾铭堂; 温淑卿; 曾筱荺; 徐士昌; 曾子豪; 庄哲仁; 黄啸谷
Original assignee: 启动生物科技股份有限公司
Priority date: 2020-12-11
Filing date: 2020-12-11
Publication date: 2022-06-16

Abstract

Antimicrobial use of an Aquilaria sinensis leaf extract and a composition thereof. The antimicrobial Aquilaria sinensis leaf extract is obtained by mixing Aquilaria sinensis leaves with a solvent to pre-prepare an Aquilaria sinensis leaf extract under extraction conditions, filtering the Aquilaria sinensis leaf extract, and drying, and is used for killing or inhibiting microorganisms. The antimicrobial composition comprises a solution of the Aquilaria sinensis leaf extract and other suitable components.

Description

Antibacterial use of agarwood leaf extract and composition thereof

technical field

The present invention relates to the use of agarwood leaves, especially the use of an agarwood leaf extract for antibacterial purposes and a composition thereof.

Background technique

The agarwood tree is a kind of agarwood plant. When its wood is damaged by external force, it will secrete resin and form a fragrant phenomenon with the wood part, thus generating the so-called "agarwood". As a medicinal use, it is also made into a standing incense for worship because of its special aroma.

technical problem

In addition to producing agarwood and having the above-mentioned uses, the agarwood tree itself has its own practical value. For example, the Chinese Taiwan Published Invention Patent No. 202033212 discloses the use of agarwood extract, which is extracted from agarwood. "Agarwood"; another example is China Taiwan Announcement No. I389701 Invention Patent Case, which discloses an extract of "Agarwood husk" and its use in the treatment of cancer; another example is China Taiwan Announcement No. I687405 Invention Patent Case, Also disclosed are an "Agarwood tree seed" extract, a preparation method and its application in anti-allergy. However, the existing uses of the agarwood tree are all in its wood, husk or seeds, and its leaves are valuable but neglected, which is a pity.

technical solutions

In order to solve the above problems, the present invention provides an antibacterial use of agarwood leaf extract and a composition thereof, wherein the agarwood leaf extract is extracted from agarwood leaves.

An embodiment of the present invention provides the use of agarwood leaf extract for antibacterial and a composition thereof. Obtained by filtration and drying of agarwood leaf extract to kill or inhibit microorganisms.

Preferably, the extraction conditions are that the agarwood leaves are mixed with a solvent in a ratio of 1:10, and extracted at a heating temperature of 100° C. for 1 hour.

Preferably, the solvent is ultrapure water.

Preferably, the filtration is to collect the supernatant after coarse filtration, and then perform fine filtration on the collected supernatant.

Preferably, the rough filtration is to perform centrifugal filtration on the agarwood leaf extract at a rotational speed of 10,000 rpm, and the filtration time is 5 minutes.

Preferably, in the fine filtration, the supernatant is first filtered with qualitative filter paper, and then filtered through a glass fiber filter.

Preferably, the pore size of the glass fiber filter membrane is 1.2 μm.

Preferably, the drying is to put the filtered agarwood leaf extract into a frozen state in a low temperature environment, and then freeze-dry it into powder before storing.

Preferably, the temperature of the low temperature environment is minus 20°C.

Preferably, the microorganism is Staphylococcus aureus, and the minimum inhibitory concentration of the agarwood leaf extract is 6.25 mg/mL.

An embodiment of the present invention provides an antibacterial composition, comprising the aforementioned solution of agarwood leaf extract, water, glycerin (Glycerin), urea (Urea), and allantoin (Allantoin), etc. The weight percentages are respectively: 40%-55% of agarwood leaf extract solution, 35%-50% of water, 1%-5% of glycerol, 0.5%-1.5% of urea and 0.05%-0.3% of allantoin.

Another embodiment of the present invention provides an antibacterial composition, comprising the aforementioned solution of agarwood leaf extract, water, 1,3-butanediol, xanthan gum, etc., wherein the weight percentages are respectively : Agarwood leaf extract solution 40% to 55%, distilled water 35% to 50%, 1,3-butanediol 1% to 8%, and xanthan gum 0.1% to 0.8%.

Thereby, the present invention extracts agarwood leaves to obtain agarwood leaf extract, which has been proved by experiments that the use of agarwood leaf extract for antibacterial purposes can indeed effectively kill or inhibit microorganisms to achieve antibacterial effects, and use this as agarwood incense. New uses for leaf applications.

Description of drawings

Fig. 1 is the extraction process of the agarwood leaf extract of the embodiment of the present invention.

Description of reference numerals

Steps S1~S4.

BEST MODE FOR CARRYING OUT THE INVENTION

In order to facilitate the description of the central idea of the present invention expressed in the column of the above-mentioned summary of the invention, it is now expressed with specific embodiments.

The present invention provides an antibacterial use of agarwood leaf extract and a composition thereof. Antibacterial in the present invention refers to the ability to kill or inhibit microorganisms. The extraction process of the agarwood leaf extract is shown in Figure 1. First, the agarwood leaves are mixed with a solvent (as in step S1), and an agarwood leaf extract is pre-prepared under an extraction condition (as in step S2), and then The agarwood leaf extract is obtained by filtering and drying (such as steps S3 and S4), and the agarwood leaf extract is used to kill or inhibit microorganisms for antibacterial purposes.

Preferably, in the extraction conditions, the agarwood leaves and a solvent are mixed in a ratio of 1:10, and the extraction is performed at a heating temperature of 100 ° C for 1 hour, and the solvent is preferably ultrapure water. For example, but not limited thereto, the solvent can also be wine.

Preferably, the filtration is to collect the supernatant after coarse filtration, and then perform fine filtration on the collected supernatant. The coarse filtration is to perform centrifugal filtration on the agarwood leaf extract at a rotational speed of 10,000 rpm, and the filtration time is 5 minutes; the fine filtration is to first filter the supernatant with qualitative filter paper (use Whatman NO.4 filter paper) Filter, and then filter through a glass fiber filter, the pore size of the glass fiber filter is 1.2 μm.

Preferably, the drying is to put the filtered agarwood leaf extract in a low temperature environment to a frozen state, and then freeze-dry it into powder and store it (for example, place it in a medicine cabinet). The temperature of the low temperature environment The best temperature is minus 20°C.

In order to illustrate the antibacterial use of the agarwood leaf extract of the present invention, an antibacterial test was carried out with Staphylococcus aureus (ATCC 6538) as a test strain. Published standard, "Minimum Inhibitory Concentration (Minimum Inhibitory Concentration)" Inhibitory Concentration, MIC)", the test equipment includes a precision balance, a biological safety cabinet, and a bacterial incubator, and the test is carried out at a temperature of 23°C and a relative humidity of 62%.

The above-mentioned antibacterial test is carried out, including the following steps:

1. In a 96-well microplate (8 rows in total, 12 wells in each row), add 100 μL of sterile M-H medium (or MH broth) to the 1st to 12th wells of each row;

2. With 100 μL of the M-H medium in the 1st to 12th wells of each row, add 100 μL of the solution containing the Agarwood leaf extract from the first well of each row, and mix with the original 100 μL of the M-H medium in the first well. After mixing, it becomes a 200 μL mixed solution. Aspirate 100 μL of the mixed solution from the first well and add it to the second well of each row, and evenly mix it with the original 100 μL of the M-H medium in the second well to form a 200 μL mixed solution. Pipette 100 μL of the mixed solution from the second well and add it to the third well of each row, and so on for doubling dilution to the 10th well. Finally, take 100 μL of the mixed 200 μL mixed solution from the 10th well, leaving only 100 μL of the mixed solution. The mixed solution of the doubling dilution is in the 10th well;

3. Add 100 μL of Staphylococcus aureus solution to the 1st to 11th wells of each row, and add 100 μL of the drug solution to the 12th well of each row. The 11th well of each row is used as the experimental group, and the 12th well of each row is used as the control. Group;

4. The aforementioned 96-well microplate was placed in a bacterial incubator, and after culturing at a constant temperature of 35°C for 18 hours, the experimental group had no bacterial growth and obtained a test result with a minimum inhibitory concentration of 6.25 mg/mL.

According to the results of the above antibacterial test, it can be seen that the minimum inhibitory concentration of 6.25 mg/mL can be obtained by the antibacterial test of the agarwood leaf extract of the present invention, which indeed has excellent antibacterial effect and can be used as antibacterial purposes.

The present invention further provides an antibacterial composition, comprising the solution of the agarwood leaf extract, water, glycerin (Glycerin), urea (Urea), and allantoin (Allantoin), etc., and the weight percentages of the components are respectively It is a solution of agarwood leaf extract of 40% to 55%, water 35% to 50%, glycerol 1% to 5%, urea 0.5% to 1.5% and allantoin 0.05% to 0.3% to form an antibacterial water. The water may be distilled water or other kinds of pure water.

The present invention further provides another composition for antibacterial, comprising the solution of the agarwood leaf extract, distilled water, 1,3-Butylene Glycol and Xanthan gum, wherein each The weight percentages of the components are respectively 40% to 55% of the solution of agarwood leaf extract, 35% to 50% of distilled water, 1% to 8% of 1,3-butanediol and 0.1% to 0.8% of xanthan gum. Forms an antibacterial gel.

The above-mentioned embodiments are only used to illustrate the present invention, but not to limit the scope of the present invention. All the modifications or changes that do not violate the spirit of the present invention belong to the intended protection category of the present invention.

Claims

A kind of agarwood leaf extract is used for antibacterial purposes, and it is characterized in that, the agarwood leaf extract is obtained by mixing agarwood leaves with a solvent to prepare an agarwood leaf extract under an extraction condition, and then using the agarwood leaf extract Obtained by filtration and drying to kill or inhibit microorganisms.
The antibacterial use of the agarwood leaf extract according to claim 1, wherein the extraction condition is that the agarwood leaf and a solvent are mixed at a ratio of 1:10, and 1 is extracted at a heating temperature of 100°C. Hour.
The antibacterial use of the agarwood leaf extract according to claim 1, wherein the solvent is ultrapure water.
The antibacterial use of the agarwood leaf extract according to claim 1, wherein the filtration is to collect the supernatant after coarse filtration, and then finely filter the collected supernatant.
The antibacterial use of the agarwood leaf extract according to claim 4, wherein the rough filtration is to perform centrifugal filtration on the agarwood leaf extract at a rotational speed of 10,000 rpm, and the filtration time is 5 minutes.
The antibacterial use of the agarwood leaf extract according to claim 4, wherein the fine filtration is to filter the supernatant with qualitative filter paper first, and then filter it through a glass fiber filter.
The antibacterial use of the agarwood leaf extract according to claim 6, wherein the pore size of the glass fiber filter membrane is 1.2 μm.
The antibacterial use of the agarwood leaf extract according to claim 1, wherein the drying is to put the filtered agarwood leaf extract in a low temperature environment to a frozen state, and then freeze-dry it into powder after storage.
The antibacterial use of the agarwood leaf extract according to claim 8, wherein the temperature of the low temperature environment is minus 20°C.
The antibacterial use of the agarwood leaf extract according to claim 1, wherein the microorganism is Staphylococcus aureus, and the minimum inhibitory concentration of the agarwood leaf extract is 6.25 mg/mL.
A composition for antibacterial, characterized in that, the solution, water, glycerol, urea and allantoin of agarwood leaf extract as claimed in claim 1 are included, and the weight percentages of each component are respectively: agarwood leaf The solution of the extract is 40%-55%, water 35%-50%, glycerol 1%-5%, urea 0.5%-1.5% and allantoin 0.05%-0.3%.
A composition for antibacterial, characterized in that, containing the solution of agarwood leaf extract as claimed in claim 1, water, 1,3-butanediol and xanthan gum, the weight percentages of each component are respectively It is: the solution of agarwood leaf extract 40%-55%, distilled water 35%-50%, 1,3-butanediol 1%-8% and xanthan gum 0.1%-0.8%.