WO2022119380A1 - Nouveau variant d'eca2 et utilisation associée - Google Patents
Nouveau variant d'eca2 et utilisation associée Download PDFInfo
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- WO2022119380A1 WO2022119380A1 PCT/KR2021/018229 KR2021018229W WO2022119380A1 WO 2022119380 A1 WO2022119380 A1 WO 2022119380A1 KR 2021018229 W KR2021018229 W KR 2021018229W WO 2022119380 A1 WO2022119380 A1 WO 2022119380A1
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- WIPO (PCT)
- Prior art keywords
- ace2
- converting enzyme
- angiotensin converting
- variant
- seq
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
Abstract
La présente invention concerne une variante de l'enzyme de conversion de l'angiotensine II (ECA2) ayant une affinité de liaison élevée pour et une neutralisation contre le coronavirus, une protéine de fusion la comprenant et une utilisation associée pour la prévention ou le traitement du coronavirus. Le variant de l'enzyme de conversion de l'angiotensine II (ECA2) de la présente invention présente une affinité de liaison plus élevée que la protéine ECA2 de type sauvage ainsi qu'une neutralisation remarquablement élevée contre le coronavirus, tel que l'inhibition de l'invasion et de la prolifération intracellulaires du coronavirus, et présente une neutralisation contre un large spectre de mutants de coronavirus. En tant que tel, le variant est utile pour inhiber la prolifération du coronavirus, prévenir une infection à coronavirus et traiter des symptômes d'une infection à coronavirus et peut être avantageusement utilisée en tant qu'agent, en particulier pour la prévention et le traitement de la COVID-19 qui est une pandémie en cours.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063120779P | 2020-12-03 | 2020-12-03 | |
| US63/120,779 | 2020-12-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2022119380A1 true WO2022119380A1 (fr) | 2022-06-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/KR2021/018229 WO2022119380A1 (fr) | 2020-12-03 | 2021-12-03 | Nouveau variant d'eca2 et utilisation associée |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2022119380A1 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024068777A1 (fr) * | 2022-09-28 | 2024-04-04 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Protéines ace2 modifiées présentant une activité améliorée contre le sars-cov-2 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6194556B1 (en) * | 1997-12-11 | 2001-02-27 | Millennium Pharmaceuticals, Inc. | Angiotensin converting enzyme homolog and therapeutic and diagnostic uses therfor |
| WO2018140456A1 (fr) * | 2017-01-24 | 2018-08-02 | Northwestern University | Variants actifs de bas poids moléculaire de l'enzyme de conversion de l'angiotensine ii (eca ii) |
-
2021
- 2021-12-03 WO PCT/KR2021/018229 patent/WO2022119380A1/fr active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6194556B1 (en) * | 1997-12-11 | 2001-02-27 | Millennium Pharmaceuticals, Inc. | Angiotensin converting enzyme homolog and therapeutic and diagnostic uses therfor |
| WO2018140456A1 (fr) * | 2017-01-24 | 2018-08-02 | Northwestern University | Variants actifs de bas poids moléculaire de l'enzyme de conversion de l'angiotensine ii (eca ii) |
Non-Patent Citations (3)
| Title |
|---|
| DATABASE GenPept NCBI; ANONYMOUS : "angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, partial [s - Protein - NCBI", XP055937372, retrieved from NLM * |
| HUSSAIN MUSHTAQ, JABEEN NUSRAT, RAZA FOZIA, SHABBIR SANYA, BAIG AYESHA A., AMANULLAH ANUSHA, AZIZ BASMA: "Structural variations in human ACE2 may influence its binding with SARS‐CoV‐2 spike protein", JOURNAL OF MEDICAL VIROLOGY, JOHN WILEY & SONS, INC., US, vol. 92, no. 9, 1 September 2020 (2020-09-01), US , pages 1580 - 1586, XP055888139, ISSN: 0146-6615, DOI: 10.1002/jmv.25832 * |
| KUI K. CHAN, DANIELLE DOROSKY, PREETI SHARMA, SHAWN A. ABBASI, JOHN M. DYE, DAVID M. KRANZ, ANDREW S. HERBERT, ERIK PROCKO: "Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 369, no. 6508, 4 September 2020 (2020-09-04), US , pages 1261 - 1265, XP055759385, ISSN: 0036-8075, DOI: 10.1126/science.abc0870 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024068777A1 (fr) * | 2022-09-28 | 2024-04-04 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Protéines ace2 modifiées présentant une activité améliorée contre le sars-cov-2 |
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