WO2022117901A1 - Utilisation de formes de résistance de microalgues en agriculture - Google Patents

Utilisation de formes de résistance de microalgues en agriculture Download PDF

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Publication number
WO2022117901A1
WO2022117901A1 PCT/ES2021/070858 ES2021070858W WO2022117901A1 WO 2022117901 A1 WO2022117901 A1 WO 2022117901A1 ES 2021070858 W ES2021070858 W ES 2021070858W WO 2022117901 A1 WO2022117901 A1 WO 2022117901A1
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WO
WIPO (PCT)
Prior art keywords
microalgae
resistance
forms
species
cyanobacteria
Prior art date
Application number
PCT/ES2021/070858
Other languages
English (en)
Spanish (es)
Inventor
Miguel GARCÍA GUERRERO
José Antonio DEL CAMPO CASTILLO
María SEGURA FORNIELES
Lucía MORENO GARRIDO
Original Assignee
Algaenergy, S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Algaenergy, S.A. filed Critical Algaenergy, S.A.
Publication of WO2022117901A1 publication Critical patent/WO2022117901A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03—Algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like

Definitions

  • the present invention relates to the use of resistant forms of microalgae in agriculture. Furthermore, it refers to a method that improves soil quality, plant growth and/or crop yield.
  • microalgae have immense potential to contribute to responding to the challenges facing the sector.
  • Microalgae are microorganisms that possess chlorophyll a and other pigments, which have the ability to carry out oxygenic photosynthesis.
  • This heterogeneous group includes tens of thousands of eukaryotic species with different morphology, size and habitat, as well as the prochaotic cyanobacteria, cyanophyceae or blue-green algae.
  • Microalgae inhabit fresh and brackish waters, marine systems and soils.
  • microalgae in sustainable agriculture goes beyond biostimulation. Soil quality is a determining factor in agricultural production, with some microalgae being natural inhabitants of these ecosystems, where they play an essential role in determining the health, quality and fertility of agricultural soils. In fact, microalgae are basic in all stages of soil formation, including the initial phase of the establishment of a biological crust on an abiotic mineral substrate.
  • These cells are resistant and usually have a thicker and harder envelope or wall than that of vegetative cells, from which they differentiate, as well as because they contain a reserve of nutrients. In nature, they can act as transport vectors between different geographical areas, both locally and intercontinentally.
  • Some microalgae that are part of the soil microflora are capable of developing forms of resistance. Under favorable environmental conditions, the forms of resistance germinate, developing and multiplying the resulting vegetative cells, thus giving rise to the establishment of important colonies of microalgae.
  • the set of effects translates into an increase in soil fertility and the productivity of the crops that are grown in them, as a result of a biotechnological action, which preserves the environment and avoids the undesirable addition of chemical agents. . Consequently, all of them are positive effects and results that can contribute decisively to food security and thus to one of the main UN Sustainable Development Goals, specifically number 2, ZERO HUNGER.
  • microalgae In the cultivation of microalgae, suitable media are used for the growth of each species in question. To stimulate the production of resistant forms, it is necessary to modify the culture conditions in order to induce their formation, which entails the controlled alteration of the concentration of certain nutrients (eg nitrogen, phosphorus, sulfur, iron, silicon ) or other critical culture parameters, such as agitation, air supply, light irradiance or temperature.
  • the application of forms of microalgae resistance to soils is an efficient way to improve their quality and fertility. On the one hand, to promote improvements in the production of the crops that grow on the soils treated in this way. On the other, for the transformation of mineral edaphic substrate, of an arid or desert nature, into soils for agricultural use. Additionally, the forms of resistance can be used in bioremediation actions focused on the recovery and restoration of impoverished, degraded or contaminated soils.
  • one aspect of the invention is the method for improving the productivity of agricultural crops that comprises the application of an efficient amount of forms of microalgae resistance, in solid form or in liquid solution, to the land in which the plants grow. cultivate.
  • a second aspect relates to a method for improving the health and fertility of a soil comprising applying an efficient amount of resistant forms of microalgae, in solid form or in liquid solution, to the soil.
  • a third aspect refers to a method of recovery and restoration of impoverished, degraded or contaminated soils.
  • the land where the forms of resistance are applied is not fertile land, but rather degraded land not suitable for cultivation that is recovered for agriculture.
  • efficient amount refers to the amount of the product, resistant forms of microalgae, that, when applied, is sufficient to improve crop productivity or improve soil fertility.
  • resistance forms refers to cells that are dormant, latent, quiescent, or metabolically quiescent.
  • Another aspect of the invention is a bioactive composition comprising resistant forms of microalgae in a range between 30% and 99% of the total number of cells in the composition.
  • bioactive refers to a composition that is added to the soil in order to provide basic elements for the growth of the plant or for the health of the plant or for the yield of the crops. plant fruits.
  • Another aspect of the invention refers, therefore, to the use of the resistant forms of microalgae as a bioactive composition to improve the productivity of agricultural crops and the fertility of the soil, or to recover or restore impoverished, degraded or contaminated soils.
  • Figure 1 shows a microscope image of the resistance forms of the cyanobacteria obtained according to the described process.
  • Figure 2 shows a micrograph of resistant forms of cyanobacteria in the process of germination.
  • the first aspect of the invention is the method to improve plant productivity, which comprises the application to the land or agricultural environment of an efficient amount of forms of microalgae resistance
  • a second aspect refers to a method to improve the fertility of a soil or to recover or restore impoverished, degraded or contaminated soils, which includes the application of an efficient quantity of resistant forms of microalgae to the land.
  • a third aspect of the invention refers to a bioactive composition, which comprises the resistant forms of microalgae in a range comprised between 30% and 90% of the total number of cells in the composition.
  • the bioactive composition comprises the resistance forms of microalgae and an agriculturally acceptable carrier of the resistance forms.
  • agriculturally acceptable carrier refers to a carrier that is known and accepted in the formation of formulations for use in agriculture or horticulture.
  • the forms of resistance preferably come from soil microalgae or terrestrial microalgae.
  • Soil microalgae are preferably selected species belonging to the Divisions: Chlorophyta, Cryptophyta, Cyanophyta, Euglenophyta, Heteromonyphyta and f ⁇ hodophyta.
  • Heterochyphyta More preferred among the Heterochyphyta are species of Bacillariophyceae (diatoms) and among the Cyanophyta (cyanobacteria) are strains of genera such as Anabaena and Nostoc.
  • microalgae in poor conditions for vegetative growth (suboptimal), have the ability to develop forms of resistance, which allow them to resist adverse conditions until the environmental and/or nutritional variables are again conducive to the development of vegetative cells.
  • This process can be reproduced artificially, forcing said conditions of nutritional and/or environmental stress.
  • the development of the resistance form is favored by controlling the level of phosphorus in the microalgae culture medium.
  • the development of the resistance form is favored under conditions of high carbon input to the microalgae culture medium.
  • the methods described above further comprise the steps of: a) cultivating the microalgae; b) induce the form of resistance; c) preserve the form of resistance; d) germinate the form of resistance.
  • Storage is preferably carried out in the dark. More preferably at a temperature between -20°C and 25°C.
  • the examples of the present invention demonstrate the formation of dormant forms, their possible conservation over time and their ability to germinate once they are in the right environment.
  • Example 1 Process of induction, isolation and stabilization of forms of resistance of bacillariophyceae (diatoms)
  • Cells from a diatom were cultured in standard liquid medium. After 10 days of growth, part of the vegetative cells were transferred to a medium with a low concentration (10 micromolar) of sodium nitrate and 150 micromolar of silicic acid, conditions that favor the massive production of forms of resistance. The temperature was maintained at 25°C in a thermostatted and illuminated chamber, using cycles of 12 hours of light: 12 hours of darkness.
  • the resistance forms obtained were dried with hot air at a moderate temperature, being ready for later storage. Periodic controls were carried out to verify that the resistant forms maintain their ability to germinate when placed in favorable conditions (humidity, temperature, nutrients, etc.)
  • Example 2 Process of induction, isolation and stabilization of resistant forms of cyanobacteria
  • the culture began with the transfer of the strains from solid medium (agar) to 250 mL flasks with standard culture medium maintained at 25°C and with orbital agitation.
  • the cultures of the cyanobacterial strains were subjected to nutritional stress due to phosphorus limitation.
  • the resuspended sediment was transferred in a volume of 600 mL to 2-5 L bottles with the low-phosphorus medium and cultured for 21 days. sampling every 3 days to follow its progression with measurements of absorbance at 750 nm and the formation of resistance forms by counting with a Neubauer Chamber.
  • the supernatant was removed from the centrifugation by decantation and the pellet was recovered in 600 mL of medium, repeating the process three times.
  • the resuspended sediment was transferred in a volume of 600 mL to 2-5 L bottles with the medium supplemented with carbon and cultured for 21 days, sampling every 3 days to follow the progression of the culture with absorbance measurements at 750 nm and the formation of resistance forms by counting with the Neubauer Chamber.
  • Figure 1 shows a photomicrograph of the concentrate of resistant forms of cyanobacteria.
  • the count of the resistance forms was carried out with a Neubauer chamber and the concentrate of resistance forms was centrifuged, eliminating the supernatant, leaving the sediment ready to proceed with drying and preservation tests and subsequent germination of the resistance forms.
  • the product obtained in the previous step was dried in an oven at 37°C for 24 hours. Once dried, preparations rich in resistance forms can be stored for several months in closed containers in the dark, either at room temperature (25°C) or at 4°C or even at temperatures below zero (-17°C).
  • Germination tests of the resistance forms were carried out 3 months after their production and storage. For this process to take place, it was necessary to resuspend and rehydrate the biomass of the resistant forms with complete culture medium.
  • This germination process also takes place in the soil, under conditions (moisture, temperature and nutrient availability) that allow the propagation and growth of cyanobacteria.
  • Dried resistance forms were resuspended in a small volume of complete medium.
  • the necessary amount of medium was added so that the absorbance at 750 nm was between 0.2 and 0.4.
  • the resulting resistance forms suspension was kept in a 100 mL flask with agitation in an orbital shaker and natural photoperiod.
  • Figure 2 shows the germination of the resistance form.

Abstract

La présente invention se rapporte à l'utilisation de formes de résistance de microalgues en agriculture. L'invention se rapporte également à un procédé qui améliore la productivité végétale et/ou le rendement des cultures et/ou la fertilité des sols.
PCT/ES2021/070858 2020-12-04 2021-11-29 Utilisation de formes de résistance de microalgues en agriculture WO2022117901A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES202031219A ES2913973B2 (es) 2020-12-04 2020-12-04 Uso de formas de resistencia de microalgas en agricultura
ESP202031219 2020-12-04

Publications (1)

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WO2022117901A1 true WO2022117901A1 (fr) 2022-06-09

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ES (1) ES2913973B2 (fr)
WO (1) WO2022117901A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987002660A1 (fr) * 1985-11-05 1987-05-07 Soil Technologies, Corp. Compositions microbiennes et procede de traitement de la terre
US20080236227A1 (en) * 2007-04-02 2008-10-02 Flynn Timothy M Dry land erosion control using photosynthetic nitrogen-fixing microorganisms
US10457610B2 (en) 2012-09-20 2019-10-29 Core Intellectual Properties Holdings, Llc Methods and compositions for treating soil and plants
WO2020006545A1 (fr) * 2018-06-29 2020-01-02 Profile Products, L.L.C. Système de restauration de sol
AU2019240617A1 (en) 2018-12-05 2020-06-25 Jeffrey Lae Biofertiliser Composition
US10701941B2 (en) 2017-11-10 2020-07-07 Heliae Development, Llc Biomass compositions

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012151382A1 (fr) * 2011-05-03 2012-11-08 Algae Biosciences Inc. Système d'inoculation de sol à base de micro-algues et procédés d'utilisation
AU2018101956A4 (en) * 2018-12-05 2019-01-17 Jeffrey Lae Biofertiliser composition

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987002660A1 (fr) * 1985-11-05 1987-05-07 Soil Technologies, Corp. Compositions microbiennes et procede de traitement de la terre
US20080236227A1 (en) * 2007-04-02 2008-10-02 Flynn Timothy M Dry land erosion control using photosynthetic nitrogen-fixing microorganisms
US10457610B2 (en) 2012-09-20 2019-10-29 Core Intellectual Properties Holdings, Llc Methods and compositions for treating soil and plants
US10701941B2 (en) 2017-11-10 2020-07-07 Heliae Development, Llc Biomass compositions
WO2020006545A1 (fr) * 2018-06-29 2020-01-02 Profile Products, L.L.C. Système de restauration de sol
AU2019240617A1 (en) 2018-12-05 2020-06-25 Jeffrey Lae Biofertiliser Composition

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ES2913973A1 (es) 2022-06-06
AR124226A1 (es) 2023-03-01

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