WO2022046957A3 - Cannabis dna constructs and methods of regulating gene expression in plants - Google Patents

Cannabis dna constructs and methods of regulating gene expression in plants Download PDF

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Publication number
WO2022046957A3
WO2022046957A3 PCT/US2021/047633 US2021047633W WO2022046957A3 WO 2022046957 A3 WO2022046957 A3 WO 2022046957A3 US 2021047633 W US2021047633 W US 2021047633W WO 2022046957 A3 WO2022046957 A3 WO 2022046957A3
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WO
WIPO (PCT)
Prior art keywords
plants
methods
cannabis
gene expression
dna constructs
Prior art date
Application number
PCT/US2021/047633
Other languages
French (fr)
Other versions
WO2022046957A2 (en
Inventor
Bridget PREISS
Cayla TSUCHIDA
Randall SHULTZ
Original Assignee
Arcadia Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arcadia Biosciences, Inc. filed Critical Arcadia Biosciences, Inc.
Publication of WO2022046957A2 publication Critical patent/WO2022046957A2/en
Publication of WO2022046957A3 publication Critical patent/WO2022046957A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Abstract

The present application relates to Cannabis DNA constructs and methods of regulating gene expression in plants. In particular, regulatory sequence elements from Cannabis are disclosed, including Cannabis Ubiquitin genes and functional fragments thereof, and their use in the expression of heterologous nucleotides in plant cells.
PCT/US2021/047633 2020-08-26 2021-08-26 Cannabis dna constructs and methods of regulating gene expression in plants WO2022046957A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063070642P 2020-08-26 2020-08-26
US63/070,642 2020-08-26

Publications (2)

Publication Number Publication Date
WO2022046957A2 WO2022046957A2 (en) 2022-03-03
WO2022046957A3 true WO2022046957A3 (en) 2022-04-14

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PCT/US2021/047633 WO2022046957A2 (en) 2020-08-26 2021-08-26 Cannabis dna constructs and methods of regulating gene expression in plants

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WO (1) WO2022046957A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160177404A1 (en) * 2011-08-18 2016-06-23 Courtagen Life Sciences Inc. Cannabis genomes and uses thereof
US20190352655A1 (en) * 2017-01-28 2019-11-21 Inari Agriculture, Inc. Novel plant cells, plants, and seeds
WO2019234754A1 (en) * 2018-06-07 2019-12-12 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Nucleic acid constructs and methods of using same
US20200056195A1 (en) * 2011-05-13 2020-02-20 Monsanto Technology Llc Plant regulatory elements and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200056195A1 (en) * 2011-05-13 2020-02-20 Monsanto Technology Llc Plant regulatory elements and uses thereof
US20160177404A1 (en) * 2011-08-18 2016-06-23 Courtagen Life Sciences Inc. Cannabis genomes and uses thereof
US20190352655A1 (en) * 2017-01-28 2019-11-21 Inari Agriculture, Inc. Novel plant cells, plants, and seeds
WO2019234754A1 (en) * 2018-06-07 2019-12-12 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Nucleic acid constructs and methods of using same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHRISTENSEN, AL ET AL.: "Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants", TRANSGENIC RESEARCH, vol. 5, no. 3, May 1996 (1996-05-01), pages 213 - 218, XP002725067, DOI: 10.1007/BF01969712 *
DATABASE Nucleotide ANONYMOUS : "Cannabis sativa maturase (matK) gene, complete cds; chloroplast gene for chloroplast product", XP055932463, retrieved from NCBI Database accession no. AF345317 *
U.S. NATIONAL LIBRARY OF MEDICINE: "NATIONAL CENTER FOR BIOTECHNOLOGY INFORMATION) PREDICTED: Cannabis sativa polyubiquitin-like (LOC115707819", MRNA, 18 May 2020 (2020-05-18), pages 1 - 2, XP055877760, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/nucleotide/XM_030635894.1> [retrieved on 20220201] *

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Publication number Publication date
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