WO2022039432A1

WO2022039432A1 - Western blotting module and cell migration observation module, and devices comprising same

Info

Publication number: WO2022039432A1
Application number: PCT/KR2021/010548
Authority: WO
Inventors: 김백길; 조남훈; 장연수; 강숙희
Original assignee: 연세대학교 산학협력단
Priority date: 2020-08-18
Filing date: 2021-08-10
Publication date: 2022-02-24

Abstract

A western blotting device according to the present invention separates a target through electrostimulation so that the target injected into a solvent is arranged in one direction in the solvent, or extracts the target to the outside of the solvent, and comprises: an electrode part electrically connected to an external power supply so that electrostimulation is applied to the target; a main body part which accommodates the solvent in an accommodation space provided therein and which is provided such that the target, injected into the solvent to which electrostimulation is applied, moves from the accommodation space to the outside; and an extraction part which is provided to be adjacent to the main body part so that the target moving from the accommodation space to the outside is extracted. In addition, a cell migration observation device according to the present invention comprises: a cell migration observation module in which a first solution and a second solution included in a plurality of solutions are provided to form separate layers with a cell migration layer therebetween, so that target cells moving to the cell migration layer are identified from the outside; and a fixing and supplying module which has the cell migration observation module fixed therein, and which is provided to enable the amount of a culture solution for an accommodated cell culture to be regulated in order for the culture solution to be supplied to the cell migration observation module.

Description

Western blotting module and cell movement observation module, and device including them

The present invention relates to a western blotting module for separating an object such that the object injected into a solvent through electrical stimulation is arranged in one direction in a solvent or extracting the object to the outside of a solvent, and an apparatus including the same, and more particularly A Western blotting module including a main body provided so that a solvent is accommodated in a receiving space provided therein, and an object injected into the solvent to which electrical stimulation is applied through an electrode can be moved from the receiving space to the extraction unit, and It's about the device.

In addition, the present invention relates to a cell movement observation module and an apparatus including the same, and more particularly, to a plurality of cells comprising a first solution containing a chemotaxis factor for inducing cell migration and a second solution containing target cells. The solution is injected, and the first solution and the second solution included in the plurality of solutions are provided to form a layer spaced apart from the inside with a cell migration layer interposed therebetween. It relates to a module and an apparatus including the same.

In general, it is necessary to spread and separate biomolecules such as proteins, DNA, RNA, etc. widely in a solvent using various separation solvents (e.g., Poly-Acrylamide Gel). In this case, SDS-PAGE (sodium dodecyl sulfate- polyacrylamide gel electrophoresis) can be used.

Specifically, SDS (sodium dodecyl sulfite) binds with biomolecules in the solvent to make them linear, losing their secondary, tertiary, and quaternary structures, and by giving the biomolecules a high density of negative charges, they are positively charged during electrophoresis. Make sure it is drawn towards the (+) pole. That is, biomolecules denatured with SDS can be electrophoresed on a solvent or PAGE (polyacrylamide gel) to precisely separate them from the solvent according to their charge and molecular weight.

Next, the biomolecule separated on the solvent is transferred to a predetermined transfer film or membrane (nitrocellulose film or polyvinylidene difluoride film, etc.) and fixed by blotting, so that the researcher can check the expression of the biomolecule. .

Specifically, in the transfer step, a wet transfer method can be used in which the protein is transferred while the solvent is vertically erected and filled with a buffer (or electrophoresis buffer). After that, overlap the membrane and place a filter paper and pad on both sides to allow the buffer to be sufficiently absorbed and at the same time to protect the solvent, and then place the cathode and anode at both ends.

At this time, since the protein present in the solvent is attracted to the positive electrode, the membrane is placed on the positive electrode side. When a voltage is applied, the proteins move slowly from the solvent to the membrane, ultimately allowing the protein to bind and exist on one side of the membrane.

For example, after performing SDS-PAGE in an electrophoresis device, the solvent is taken out of the device, stacked in the order of [pad-filter paper-solvent-membrane (transfer film)-filter paper-pad], and fixed with clamps (' After manipulation with a 'cassette' or 'sandwich'), blotting can be performed to transfer biomolecules in a solvent to a transcription film in the presence of a buffer solution (or a buffer for transcription).

However, conventional electrophoresis and blotting are performed manually by researchers using independent devices.

Therefore, there is a problem that the experimental results can be greatly affected by the skill of the researcher and laboratory conditions, and there is a problem that mass analysis is very difficult.

Also, in the related art, when the amount of the object used for analysis is small, there is a problem in that it is impossible to visualize the object transferred to the membrane (transfer film).

On the other hand, cell migration by chemoattractants is confirmed in various physiological and pathological phenomena.

Specifically, cancer cells secrete angiogenesis-inducing factors included in the above-described chemotactic factors, and may initiate angiogenesis by binding to receptors present in endothelial cells.

For example, endothelial cells stimulated by angiogenesis inducers begin to grow and secrete protease. It moves toward the place where the angiogenesis inducer comes out, and then, when the migrated endothelial cells are connected to each other and each endothelial cell curves (cuvature), the length of the lumen increases and a loop is formed. blood enters the

Recently, in order to discover a gene that induces or suppresses such angiogenesis or to develop a drug involved, a study of recording the above-described cell migration image is being actively conducted.

However, since the conventional module and device for observing cell movement imaging used a two-dimensional method of transferring cells from left to right or right to left to confirm cell movement on the ground and plane, the cells were classified by type by layer and step by step. There is a disadvantage in that it has limitations in various observations depending on the

In order to solve the above problems, the present invention accommodates a solvent in a receiving space provided therein, and a body provided so that an object injected into a solvent to which electrical stimulation is applied through an electrode unit can be moved from the receiving space to the extraction unit It is a challenge to provide a Western blotting apparatus comprising a portion.

In addition, when the amount of the object used for analysis is small, the main body portion is provided to face each other with respect to the accommodation space so as to enable visualization of the object transferred to the membrane, and the first side and the first side are partially open It is characterized in that it comprises a second side connected to the first side so as to be perpendicular to the first side, it is a task to provide a Western blotting module having a length shorter than the width of the second side.

In addition, the present invention is to solve the above problems, and a plurality of solutions including a first solution containing a chemotaxis for inducing cell migration and a second solution containing target cells are injected, and a plurality of The first solution and the second solution included in the solution are provided to form a layer spaced apart from the inside with a cell migration layer interposed therebetween, and a module for checking target cells moving to the cell migration layer from the outside, and a device including the same intended to provide

In addition, another object of the present invention is to provide a cell movement observation module prepared to be connected to a plurality of cells so that the cells can be observed in various ways according to the type of each layer, step by step.

The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to solve the above problems, according to one aspect of the present invention, the western blotting module includes a main body in which a solvent injected into a subject is accommodated and opened in one direction; and electrode units provided on both sides along the opening direction of the main body to extract the object to the outside of the main body.

First, the electrode unit may include: a first electrode member in which a current path for arranging the object in one direction is formed by separating the object in the solvent; a second electrode member in which a current path for moving the object injected into the solvent in an open direction of the main body is formed; and an extraction member provided adjacent to the main body to extract the object moving along the opening direction of the main body.

Here, the current path of the first electrode member and the second electrode member may be in a direction perpendicular to each other.

In this case, in the first electrode member, a current path may be formed such that the object is arranged from the top to the bottom in the solvent.

Meanwhile, the electrode part may further include a cover member provided to surround the main body part and the extraction member, and in which the first electrode member and the second electrode member are formed in the inner space.

Here, the cover member may include: a first cover provided to surround one side of the main body so that the first electrode member formed on the inner surface is electrically connected to an external power source; and a second cover that surrounds the other side of the main body and is coupled to the first cover member so that the second electrode member formed on the inner surface is electrically connected to an external power source.

In addition, the first electrode member may include: a first electrode provided to protrude above the first cover and connected to an external power source; and a first conductive plate connected to the first electrode so that different poles are formed on the upper and lower ends of the inner surface of the first cover.

In addition, the second electrode member may include: a second electrode provided to protrude above the second cover and connected to an external power supply; and a second conductive plate connected to the second electrode so that different poles are formed on the center side of the inner surface of the first cover and on the center side of the inner surface of the second cover.

In order to solve the above problems, according to one aspect of the present invention, the western blotting apparatus separates the object such that the object injected into the solvent through electrical stimulation is arranged in one direction in the solvent, or the object is subjected to the solvent An apparatus for extracting to the outside of, an electrode part electrically connected to an external power source so that electrical stimulation is applied to the object; a body part that accommodates the solvent in a accommodating space provided therein, and is provided so that the object injected into the solvent to which electrical stimulation is applied can be moved to the outside in the accommodating space; and an extraction unit which is provided adjacent to the main body and extracts the object moved to the outside in the accommodation space.

First, the main body portion may have an open outer peripheral surface corresponding to the movement direction of the object so that the object can be moved from the accommodation space to the extraction unit.

Here, the main body may be provided in a rectangular shape elongated in the vertical direction.

In addition, the main body portion is provided to face each other with respect to the receiving space, a first side of which is formed openly; and a second side surface connected to the first side surface so as to be perpendicular to the first side surface, and the first side surface may have a length shorter than a width of the second side surface.

Here, in the first side surface, a cut-out hole symmetrical to each other with respect to the accommodation space may be formed.

In this case, the incision hole may be formed in the first side surface so that the object can be moved to the extraction unit in a direction perpendicular to the longitudinal direction of the body unit.

In addition, the cut-out hole may be provided in a rectangular shape that is formed long in the vertical direction.

Here, when the object is arranged from the top to the bottom inside the solvent by electrical stimulation, the incision hole has a length corresponding to the arrangement and width of the object so that the arranged object can be transferred to the extraction unit. and width.

In addition, the main body may be provided with an adjustment member for adjusting the length and width of the cut-out hole.

In addition, the main body portion may be connected to a plurality of objects in a width direction of the first side so that a plurality of objects can be transferred to the extraction unit.

Meanwhile, the electrode unit may include: a first electrode member in which a current path for arranging the object in one direction is formed by being separated in the solvent; and a second electrode member in which a current path for moving the object injected into the solvent from the accommodation space to the extraction unit is formed.

Here, in the first electrode member, a current path for arranging the object in the solvent from the top to the bottom may be formed.

Next, the electrode part may further include a cover member provided to surround the body part and in which the first electrode member and the second electrode member are formed in the inner space.

In this case, the first electrode member may include: a first electrode provided to protrude above the first cover and connected to an external power source; and a first conductive plate connected to the first electrode so that different poles are formed on the upper and lower ends of the inner surface of the first cover.

Furthermore, the Western blotting apparatus according to an embodiment of the present invention may further include a fixing part for arranging the plurality of electrode parts in a current path direction of the second electrode member.

In addition, so that the object is separated and arranged in one direction in the solvent by electrical stimulation, the buffer for electrophoresis, which has one end and the other end of the solvent having different potentials, is introduced into or discharged from the electrode part, or is added to the solvent by electrical stimulation. It may further include a receiving unit accommodating the electrode unit so that a transfer buffer for forming a flow of ions is drawn into or discharged from the inside of the electrode unit so that the injected object can be moved from the receiving space to the extraction unit.

In addition, it may further include a switch provided on the receiving part, electrically connected to the electrode part to control the direction of electrical stimulation applied from the electrode part to the main body part.

In order to solve the above problems, according to one aspect of the present invention, a cell movement observation device includes a plurality of solutions including a first solution containing a chemotaxis factor for inducing cell migration and a second solution containing target cells As the injected cell migration observation device, the first solution and the second solution contained in the plurality of solutions are provided to form a layer spaced apart from the inside with a cell migration layer interposed therebetween, and move to the cell migration layer a cell movement observation module for externally checking the target cell; and a fixed supply module for supplying the culture solution to the cell migration observation module by fixing the cell movement observation module therein and adjusting the amount of the culture medium for cell culture accommodated therein.

Here, the cell movement observation module includes: a body member provided with an accommodating space for accommodating the plurality of solutions therein; an injection member provided such that the plurality of solutions are injected into positions arranged at a predetermined interval on the body member and moved to the receiving space at different heights; and connecting members provided on both sides of the body member so that the plurality of body members are connected to each other or the body member is fixed to the fixed supply module.

In this case, the connecting member may include: irregularities protruding from one side of the body member; and a recess corresponding to the shape of the unevenness on the other side surface of the body member.

In addition, the accommodating space may be provided in a rectangular shape elongated in the vertical direction to accommodate the plurality of solutions.

In addition, the main body member may be provided with a cut-out groove communicating with the lower portion of the accommodation space.

Next, the injection member may include a plurality of injection holes arranged at predetermined intervals on the upper surface of the body member so that the plurality of solutions can be injected.

The injection member may be connected to a side surface of the accommodation space by being bent toward the accommodation space inside the main body member by connecting a plurality of transfer pipes to the lower end of the injection hole in correspondence to the arranged positions of the injection holes.

Next, in the fixed supply module, on one side of the inner side, a fixture introduced into the groove is formed to protrude in a vertical direction from the lower surface, and a fixing groove introduced into the unevenness on the other side of the inner side may be formed in a vertical direction from the lower surface. there is.

In addition, the fixed supply module may include a partition member having one side connected to the fixture and the other end connected to the fixing groove in order to adjust the amount of the culture solution accommodated.

Here, in the cell movement observation module having the configuration as described above, a rectangular receiving space formed long in the vertical direction to accommodate the solution is provided, and a cut-out groove provided in the lower portion of the receiving space is provided to communicate with the receiving space. a body member; A plurality of injection holes into which the solution is injected are arranged at a predetermined interval on the upper surface of the main body member, and a plurality of transfer pipes connected to the lower end of the injection holes corresponding to the respective positions of the arranged injection holes are formed inside the body member. an injection member bent toward the accommodating space and connected to a side surface of the accommodating space; and a connection member provided with concavities and convexities protruding from one side of the body member and grooves formed to correspond to the shape of the concavities and convexities on the other side of the body member to be connected to the object.

In this case, the injection member may be connected to a side surface of the accommodation space at different heights from a plurality of transfer pipes based on the lower surface of the accommodation space.

In addition, the body member may be provided so that the inside of the accommodation space can be checked from the outside.

Furthermore, the injection member may be provided so as to check the solution moving from the outside of the main body member to the inside of the transfer pipe.

On the other hand, according to another aspect of the present invention for solving the above problems, the cell movement observation device is a plurality of cells including a first solution containing a chemotaxis for inducing cell migration and a second solution containing a target cell A cell migration observation device into which a solution is injected, wherein the first solution and the second solution included in the plurality of solutions are provided to form a layer spaced apart from each other with a cell migration layer therebetween, and move to the cell migration layer a cell movement observation module for confirming the target cell from the outside; and a receiving module for accommodating a culture solution for cell culture in order to supply the culture solution to the cell movement observation module; and a fixing module for fixing the cell movement observation module inside the accommodation module.

At this time, the cell movement observation module, the body member provided with a receiving space for accommodating the plurality of solutions therein; and an injection member provided so that the plurality of solutions are injected into positions arranged at a predetermined interval on the body member and moved to the receiving space at different heights.

In addition, the injection member may be provided with a plurality of injection holes arranged at predetermined intervals on the upper surface of the main body member so that the plurality of solutions may be injected.

At this time, the injection member is formed with a plurality of transfer pipes connected to the lower end of the injection hole in correspondence with the respective positions of the arranged injection holes, bent toward the accommodation space inside the body member, and connected to the side of the accommodation space. .

In this case, in the cell movement observation module according to another aspect of the present invention for solving the above problems, a main body member and a plurality of the injection member may be formed and arranged in one direction.

Next, the fixing module may include a plurality of fixing members that protrude upward from the lower surface and are arranged in one direction so that the cell movement observation module can be introduced into the interspace.

In addition, the fixing module may be formed with a holding member protruding upward from both sides of the lower surface so as to be arranged in parallel with the fixing member so that the ends are bent in opposite directions.

In addition, in the fixing module, a culture solution supply path for introducing the culture solution into the second solution along the incision groove of the cell movement observation module passes through the fixing member and the holding member in a direction perpendicular to the longitudinal direction of the fixing member. can be formed.

According to the western blotting module of the present invention and an apparatus including the same, there is an advantage that large-scale analysis is possible without being greatly affected by the experimental results by the skill of the researcher and the laboratory conditions.

In addition, the Western blotting module of the present invention and an apparatus including the same have an advantage in that it is possible to visualize the object transferred to the membrane even when the amount of the object used for analysis is small.

In addition, according to the cell migration observation module of the present invention and a device including the same, a plurality of solutions including a first solution containing a chemotaxis factor for inducing cell migration and a second solution containing target cells are injected, The first solution and the second solution included in the plurality of solutions are provided to form layers spaced apart from the inside with the cell migration layer interposed therebetween, and the target cells moving to the cell migration layer are removed from the outside according to the type of each layer by step. It has the effect of being able to observe in a variety of ways.

Effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

The summary set forth above as well as the detailed description of preferred embodiments of the present application set forth below may be better understood when read in conjunction with the accompanying drawings. For the purpose of illustrating the invention, there are shown in the drawings preferred embodiments. It should be understood, however, that the present application is not limited to the precise arrangements and instrumentalities shown.

1 is a view showing an overall state in which a body part is combined inside an electrode part in a western blotting module according to an embodiment of the present invention;

Figure 2 is a view showing an exploded perspective view of the electrode portion and the body portion in the Western blotting module according to an embodiment of the present invention;

3 is a view showing a first cover of the cover member of the western blotting module according to an embodiment of the present invention;

Figure 4 is a view showing a second cover of the Western blotting module according to an embodiment of the present invention;

5 is a view showing a state in which the cover member of the Western blotting module is coupled according to an embodiment of the present invention;

Figure 6 is a view showing the overall appearance of the Western blotting apparatus according to an embodiment of the present invention;

7 is a view showing an exploded perspective view of the main body of the Western blotting apparatus according to an embodiment of the present invention;

8 is a view illustrating a state in which a solvent injected with an object is accommodated in the main body of the Western blotting apparatus according to an embodiment of the present invention;

9 is a view for explaining the first surface and the second surface and the transfer direction of the main body of the Western blotting apparatus according to an embodiment of the present invention;

10 is a view showing the first side of the main body of the Western blotting apparatus according to an embodiment of the present invention has a length shorter than the width of the second side, so that the solvent accommodated in the main body and the object (b) injected into the solvent are conventionally shown. A drawing for explaining a state in which a very small amount of subject transfer is possible compared with the solvent of the technology and the subject (a) injected into the solvent;

11 is a view for explaining a fixing part for arranging a plurality of electrode parts of the main body of the Western blotting apparatus in the current path direction of the second electrode member according to an embodiment of the present invention;

12 is a view for explaining a switch unit coupled to the upper portion of the receiving unit of the Western blotting apparatus according to an embodiment of the present invention;

13 is a view for explaining a state in which the current path of the electrode part is changed according to the sliding of the switch part of the Western blotting apparatus according to an embodiment of the present invention;

14 is a view showing the appearance of a cell movement observation device according to an embodiment of the present invention;

15 is a view showing a state of the cell movement observation module of the cell movement observation device according to an embodiment of the present invention;

16 is a diagram illustrating a state in which target cells included in the second solution move in the opposite direction to gravity by the chemotaxis factor included in the first solution in the cell migration observation module of the cell migration observation device according to an embodiment of the present invention; drawing;

17 is a view showing the appearance of a cell movement observation device according to another embodiment of the present invention;

18 is a view showing a state of the cell movement observation module of the cell movement observation device according to another embodiment of the present invention;

19 is a view for explaining a state in which the first solution, the hydrogel, and the second solution are stacked in the cell movement observation device according to another embodiment of the present invention;

20 is a cell movement observation module inserted between the fixing members of the fixing module in the cell movement observation device according to another embodiment of the present invention and fixed, and the culture medium is received along the incision groove of the cell movement observation module through the culture medium supply path. It is a drawing showing the state of entering the space.

*<Explanation of code>

10: Western blotting module

200: body part

400: electrode part

420: first electrode member

440: second electrode member

460: extraction member

20: Western blotting apparatus

1200: electrode part

1400: body part

1600: extractor

1700: fixed part

1800: receptacle

1900: switch unit

A1: first solution

A2: second solution

H: hydrogel

C: culture medium

M: membrane

30: cell movement observation device

500: cell movement observation module

520: body member

522: accommodation space

522a: scale

524: incision groove

540: injection member

542: inlet

544: transfer pipe

560: connecting member

562: irregularities

564: Yohom

600: fixed supply module

620: fixed groove

640: fixture

660: bulkhead

40: cell movement observation device

2200: cell movement observation module

2400: accommodation module

2420: cover

2600: fixed module

Hereinafter, preferred embodiments of the present invention in which the object of the present invention can be specifically realized will be described with reference to the accompanying drawings. In describing the present embodiment, the same names and the same reference numerals are used for the same components, and an additional description thereof will be omitted.

1 is a view showing an overall state in which a main body is combined inside an electrode in a western blotting module according to an embodiment of the present invention, and FIG. 2 is an electrode in the western blotting module according to an embodiment of the present invention and FIG. It is a view showing an exploded perspective view of the main body, Figure 3 is a view showing the first cover of the cover member of the Western blotting module according to an embodiment of the present invention, Figure 4 is a Western blotting according to an embodiment of the present invention It is a view showing a second cover of the module, and FIG. 5 is a view showing a state in which the cover member of the western blotting module according to an embodiment of the present invention is coupled.

The subject described in the present invention is injected into various separation solvents (eg, Poly-Acrylamide Gel) and spread widely in the solvent by unidirectional electrical stimulation to separate proteins, DNA, or any one of biomolecules including RNA can be

In addition, the buffer solution for electrophoresis described in the present invention serves to supply positive ions to the object to draw the negatively charged object in one direction, lower the pH, and prevent decomposition of the object during electrophoresis, As long as the object can play a role of making the object uniformly negatively charged, the components may vary, and thus the scope of rights is not limited.

In addition, if the buffer for transcription for Western blotting described in the present invention is accommodated in the receiving unit and electrical stimulation is applied in the one direction and the other direction as described above to perform a role for transferring the object to the extraction member or the extraction unit, the components may vary. and, therefore, the scope of rights is not limited.

However, it will be convenient for the transfer buffer to include NuPAGE® Transfer Buffer, NuPAGE® antioxidant, methanol, and deionized water.

As shown in FIG. 1 , the Western blotting module 10 according to an embodiment of the present invention may largely include a main body 200 and an electrode 400 .

First, the body part 200 is opened in one direction so that the object can be extracted to the outside of the body part 200 by the electrode part 400 , and can serve to accommodate the solvent injected into the object.

Here, the main body 200 has an accommodation space for accommodating the object, the solvent injected with the object is accommodated in the accommodation space, and the object is provided outside the main body 200. The electrode 400 to be described later is extracted. An outer peripheral surface corresponding to the moving direction of the object may be opened to be moved by the member.

At this time, the main body 200 may be provided in the form of a rectangle elongated in the vertical direction, and the receiving space formed therein may also be provided in the form of a rectangle elongated in the vertical direction.

At this time, the solvent accommodated in the accommodation space may be pre-solidified in response to the shape of the accommodation space after injection of the object.

In addition, the solvent may be provided to close the face open in one direction from the main body 200 and accommodated in the receiving space, and the object may be injected to solidify in response to the shape of the receiving space, and the shape of the solvent corresponds to the shape of the receiving space It goes without saying that the scope of rights is not limited by this, as long as it can be formed.

In addition, the main body 200 may include a first side surface and a second side surface, and specifically, the first side surface may be provided to face each other based on the accommodation space by being partially open.

Here, the second side is connected to the first side, and may be connected to be perpendicular to the first side.

In this case, the first side may have a length shorter than the width of the second side, and the first side has a symmetrical cut-out hole formed with respect to the receiving space, and the Western blow according to an embodiment of the present invention is formed through the cut-out hole. Western blotting, that is, transcription, which will be described once again, can be performed in a printing apparatus.

Specifically, the incision hole may be formed in a rectangular shape elongated in the vertical direction so that the object can be moved to the extraction unit in a direction perpendicular to the longitudinal direction of the main body 200 .

Here, when the object is arranged from the top to the bottom inside the solvent by electrical stimulation for electrophoresis, the incision hole has a length and width corresponding to the arrangement and width of the object so that the arranged object can be transferred to the extraction unit. can have

Due to this, even when the amount of the object is very small, visualization of the object extracted by the extraction member may be possible, and the Western blotting apparatus according to an embodiment of the present invention will be described in detail.

At this time, although not shown in the drawings, the main body 200 may further include an adjusting member for adjusting the length and width of the incision hole to adjust the length according to the arrangement of the object and adjust the width according to the amount of the object.

Here, the adjustment member is formed to slide on one side of the incision hole, and it will be convenient to provide the length and width of the incision hole to be adjusted.

The main body 200 having the configuration as described above may be connected to a plurality of objects in the width direction of the first side so that a plurality of objects can be transferred to the extraction member.

In other words, electrical stimulation is applied to a plurality of solvents accommodated in one main body 200 , and each of a plurality of objects injected into the plurality of solvents may be all arranged in one direction.

In the case in which a plurality of body parts 200 are connected in the width direction of the first side, although not shown in the drawings, the body part 200 is supported on the bottom surface to accommodate the solvent so that the solvent can be stably accommodated. It may be fixed through a fixing holder for, and the fixing holder may be provided to hold one side of the main body 200 and cover the other side of the main body 200 with a cover.

Next, the electrode part 400 may be provided on both sides along the open direction of the body part 200 to serve to extract the object to the outside of the body part 200 .

Specifically, referring to the exploded perspective view shown in FIG. 2 , the electrode part 400 may largely include a first electrode member 420 , a second electrode member 440 , and an extraction member 460 .

Here, the first electrode member 420 may be provided to form a current path for arranging the object in one direction by separating the object in the solvent.

That is, a current path for performing electrophoresis may be formed so that electrical stimulation in one direction is applied to the main body 200 .

In this case, in the second electrode member 440 , a current path for moving the object injected into the solvent in the open direction of the main body 200 may be formed.

That is, a current path for performing the transfer may be formed so that electrical stimulation in one direction and the other direction is applied to the main body 200 .

In addition, the extraction member 460 is provided adjacent to the main body 200 so that an object moving along the open direction of the main body 200 can be extracted.

In this case, the current paths of the first electrode member 420 and the second electrode member 440 may be in a direction perpendicular to each other, and in this case, the first electrode member 420 is arranged such that the object is arranged in the solvent from the top to the bottom. , a current path may be formed.

Although not shown in the drawings, the extraction member 460 is provided to enable wireless communication with the outside, and may further include a photographing module for photographing the object extracted by the extraction member 460 and transmitting the photographed image to the outside. .

Specifically, when an external user generates a photographing image request through an external terminal and transmits it to the external management server, the external management server transmits a control command for photographing the object extracted by the extraction member 460 to the photographing module, and photographing The module may receive the control command of the external management server, photograph the extracted object with the extraction member 460, and transmit the captured object to the external management server.

Finally, the external management server converts the received photographed image into data and transmits the photographed image to an external terminal that has generated a photographed image request, so that the extraction member 460 is not removed from the electrode unit 400 and remotely through an external terminal. It may be provided to confirm the transcription result.

The electrode unit 400 as described above may further include a cover member 480 , the cover member 480 is provided to surround the main body 200 and the extraction member 460 , and the first An electrode member 420 and a second electrode member 440 may be formed.

For example, the extraction member 460 is formed on one side in the transfer direction with the main body 200 as the center inside the cover member 480, and the main body 200 and the extraction member 460 are wrapped with filter paper (S1). Next, the pad S2 is provided to wrap the filter paper S1 again to protect the solvent from external stimuli, and the object separated from the solvent by sufficiently absorbing the buffer in the transfer direction is moved slowly to the extraction member 460 to the extraction member. An object may be coupled to one surface of 460 .

Here, the cover member 480 may include a first cover 482 and a second cover 484 .

The first cover 482 is provided to surround one side of the main body 200 and serves to fix the first electrode member 420 so that the first electrode member 420 formed on the inner surface is electrically connected to an external power source. can do.

The second cover 484 surrounds the other side of the main body 200 and is coupled to the first cover 482 so that the second electrode member 440 formed on the inner surface is electrically connected to the external power source. ) can play a role in fixing

3 , the first electrode member 420 may include a first electrode 422 and a first conductive plate 424 .

First, the first electrode 422 is provided to protrude above the first cover 482 and may be connected to an external power source.

In this case, the first conductive plate 424 may be connected to the first electrode 422 so that different poles are formed on the upper end and the lower end of the inner surface of the first cover 482 .

Specifically, the negative electrode of the first electrode 422 is provided to protrude to the upper left side of the first cover 482 , and the first conductive plate 424 is elongated in the vertical direction on the inner surface of the first cover 482 in the vertical direction. It may be formed and connected to the cathode of the first electrode 422 .

Here, the upper portion of the first cover 482 on which the first conductive plate 424 connected to the cathode of the first electrode 422 is formed is rotated along an imaginary axis perpendicular to the vertical direction so that the upper portion of the cover member 480 is opened. can be provided.

At this time, a buffer solution for electrophoresis may be injected into the upper portion of the rotating cover member 480 .

At this time, the anode of the first electrode 422 is provided to protrude from the upper right side of the first cover 482 , and another first conductive plate 424 bent in a 'L' shape is formed as the anode of the first electrode 422 . can be connected to

Specifically, another first conductive plate 424 connected to the anode of the first electrode 422 is spaced apart from the end of the first conductive plate 424 connected to the cathode of the first electrode 422 to be spaced apart from the first electrode 422 . ) connected to the anode, the first cover 482 is formed long downward along the side surface, and the end thereof may be bent to be parallel to the first conductive plate 424 connected to the cathode.

Accordingly, a current path for electrophoresis is formed from the bottom to the top, and the object injected into the solvent may be arranged from the bottom to the bottom.

In addition, the second conductive plate 444 connected to the second electrode 442 is also provided on the inner central side of the first cover 482 so as to form a current path for transfer, which will be described later, inside the first cover 482 . A first connector 444a may protrude toward the second cover 484 on the second conductive plate 444 provided on the central side.

Next, the second electrode member 440 may include a second electrode 442 and a second conductive plate 444 .

First, the second electrode 442 may be provided to protrude above the second cover 484 to be connected to an external power source.

In this case, the second conductive plate 444 may be connected to the second electrode 442 so that different poles are formed on the center side of the inner surface of the first cover 482 and the center side of the inner surface of the second cover 484 .

As shown in FIG. 4 , specifically, the anode of the second electrode 442 is provided to protrude from the upper left side of the second cover 484 , and the anode of the second electrode 442 is located on the center side of the second cover 484 . A connected square second conductive plate 444 may be formed.

In addition, the negative electrode of the second electrode 442 is provided to protrude from the upper right side of the second cover 484 , and extends downward of the second cover 484 at the lower end of the negative electrode of the second electrode 442 , and then the first cover A second connector 444b bent toward the first cover 482 may be provided to be connected to the first connector 444a provided in the 482 .

Accordingly, as shown in FIG. 5 , when the first cover 482 and the second cover 484 are coupled, electrophoresis is performed in the vertical direction, and transfer is performed in a direction perpendicular to the vertical direction.

A Western blotting apparatus for performing electrophoresis and transcription including the Western blotting module as described above will be described as follows.

6 is a view showing the overall appearance of a western blotting apparatus according to an embodiment of the present invention, FIG. 7 is an exploded perspective view of a main body of a western blotting apparatus according to an embodiment of the present invention, and FIG. 8 is a view showing a state in which a solvent injected with an object is accommodated in the main body of the Western blotting apparatus according to an embodiment of the present invention, and FIG. It is a view for explaining the first side and the second side and the transfer direction, and FIG. 10 (a) is a view showing a state in which a very small amount of an object is transferred in a solvent accommodated in a device prepared in the prior art, FIG. 10 (b) is a view showing a state in which a very small amount of the object is transferred from the main body portion having a length shorter than the width of the first side of the second side of the Western blotting apparatus according to an embodiment of the present invention, Figure 11 is an embodiment of the present invention A view for explaining a fixing unit for arranging a plurality of electrode units in the main body of the Western blotting apparatus in the direction of the current path of the second electrode member according to an embodiment, and FIG. 12 is a Western blotting apparatus according to an embodiment of the present invention. It is a view for explaining a switch unit coupled to the upper portion of the receiving unit, and FIG. 13 is a view for explaining a state in which the current path of the electrode unit is changed according to the sliding of the switch unit of the Western blotting apparatus according to an embodiment of the present invention .

As shown in FIGS. 6 and 7 , the Western blotting apparatus 20 according to an embodiment of the present invention separates the object so that the object injected into the solvent through electrical stimulation is arranged in one direction in the solvent, or As an apparatus for extracting the solvent to the outside, it may largely include an electrode part 1200 , a body part 1400 , and an extraction part 1600 .

First, the electrode unit 1200 is electrically connected to an external power source and can serve to apply electrical stimulation to the object, and includes the first electrode member and the second electrode member described above, and includes the cover member can

That is, the electrode unit 1200 of the Western blotting apparatus is an extraction member provided adjacent to the main body so that an object moving along the open direction of the main body is extracted from the electrode 400 described through the Western blotting module 10 . Except for 460 , the rest of the configuration and function are the same and similar, so the description of the shape of the electrode part 1200 will be omitted.

The main body 1400 accommodates the solvent in the receiving space provided therein, and may be provided so that an object injected into the solvent to which electrical stimulation is applied can be moved from the receiving space to the outside, and the object is moved from the receiving space to the extraction unit. It may be provided so that an outer peripheral surface corresponding to the moving direction of the object can be opened.

The main body 1400 is also provided with a first side 1420 and a second side 1440 similar to the function and shape of the main body 200 described above in the Western blotting module 10, and the first side 1420 ) is opened to provide an incision groove 1422, and a plurality of connections are formed in the width direction of the first side surface 1420 as shown in FIG. 8 to accommodate the solvent G injected with the object D. Therefore, a description of the shape thereof will be omitted and only the part to be described later will be described.

The extraction unit 1600 may be a membrane (or transfer film) that is provided adjacent to the main body 1400 and is extracted by coupling the object D moving to the outside in the receiving space, and has been described through an embodiment of the present invention. Since the extraction member 460 has the same function and structure, a detailed description thereof will be omitted, and the length of the first side 1420 of the main body 1400 to be described later is shorter than the width of the second side 1440 . The effect of having , will be described in detail as follows.

For example, the body part 1400 is formed to be elongated in the vertical direction, and electrophoresis is performed in the solvent accommodated in the body part 1400 by electrical stimulation of the electrode part 1200 , so that the object is arranged in a downward direction in the solvent. Then, as shown in FIG. 9, along the cutout groove 1422 formed in the first side 1420 of the main body 1400, electrical stimulation for transfer is applied to the main body 1400 in a direction perpendicular to the vertical direction. can

Figure 10 (a) showing a state that a very small amount of the object (D) is transferred in the solvent accommodated in the apparatus prepared in the prior art and the first side 1420 of the Western blotting apparatus 20 according to an embodiment of the present invention As shown in (b) of FIG. 10 showing a state in which a very small amount of the object D is transferred from the body portion 1400 having a length shorter than the width of the second side surface 1440, the object D is an extraction unit (1600) can be transcribed.

Here, in FIGS. 10 (a) and (b), when electrical stimulation for transcription is applied in both cases, the main body 1400 is omitted and the same very small amount of the solvent accommodated in the main body 1400 is the object (D) is injected and transferred to the extraction unit 1600 , and is a diagram illustrating a process in which the object D is coupled to the extraction unit 1600 .

Here, the length of one side of the object D injected into the solvent G formed to correspond to the shape of the device prepared in the prior art shown in FIG. 10 (a) and the second aspect of the present invention shown in FIG. 10 (b) It is assumed that the length of one side of the object D injected into the solvent G received in contact with 1440 is the same as d2, and w1, which is the length of the other side of the solvent corresponding to the transfer direction in the device prepared in the prior art, is It can be assumed that it is equal to the length of d2.

At this time, since the length of the first side 1420 of the present invention is shorter than that of the second side 1440 , the length w2 of the contacting solvent G and the injected object may also be shorter than the length w1 and d2 .

In addition, when the same amount of the object is injected into the solvent, the volume of the formed object is the same, so the height h1 according to an embodiment of the present invention is higher than the height h1 that can be confirmed when the object is transferred from the apparatus prepared in the prior art to the extraction unit 1600 When the device is transferred to the extraction unit 1600, the height h2 that can be confirmed may be formed high.

That is, the object coupled to the extraction unit 1600 may be formed to be higher in the vertical direction and to be shorter in length in the vertical direction than in the prior art.

In other words, compared with the object D coupled to the extraction unit 1600 with a width of l1 and a height of h1 in a device prepared in the prior art, the object D coupled to the extraction unit 1600 of the present invention is l1 It has a shorter l2 width and a higher h2 height than h1.

In summary, the first side 1420 of the main body 1400 of the present invention is formed shorter than the second side 1440, and the solvent accommodated therein also corresponds to the shape of the first side 1420 and the second side 1440. By being prepared, when a very small amount of the object is injected into the solvent, visualization of the object D extracted from the apparatus according to an embodiment of the present invention may be easier than the object D extracted from the apparatus prepared in the prior art.

At this time, by adjusting as described above, the width of the incision groove 1422 may be adjusted in response to the amount injected into the solvent, or the length of the incision groove 1422 may be adjusted in response to the length arranged in one direction by electrical stimulation on the solvent. .

The Western blotting apparatus according to an embodiment of the present invention having the configuration as described above may further include a fixing unit 1700 .

11, the fixing part 1700 serves to arrange the plurality of electrode parts 1200 in which the main body part 1400 and the extraction part 1600 are provided in the direction of the current path of the second electrode member. can be done

Specifically, the fixing unit 1700 may be provided in a rectangular shape so that the lower surface of the electrode unit is in contact with the lower surface 1720 .

In addition, the fixing part 1700 is formed from the upper central side to the lower surface 1720 of the fixing part so that the electrode part 1200 can be introduced, and a plurality of lead-in grooves arranged in the current path direction of the second electrode member may be provided. there is.

In addition, the fixing unit 1700 may be provided with a handle on one side and the other side of the upper portion.

In addition, the fixing part 1700 may be provided with an upper side partially open with respect to the lower surface 1720 so that a buffer solution for electrophoresis or a buffer solution for transfer can be introduced into the electrode part.

The Western blotting apparatus having the above-described configuration may further include a receiving unit 1800 .

In the receiving unit 1800, the buffer solution for electrophoresis, which causes one end and the other end of the solvent G to have different potentials, is introduced into the electrode unit 1200 or discharged from the inside of the electrode unit 1200, or the solvent is stimulated by electrical stimulation. A transfer buffer for forming a flow of ions is introduced into the electrode part 1200 or the electrode part 1200 so that the object (D) injected into (G) can be moved from the receiving space to the extraction part 1600 . It may serve to accommodate the electrode part 1200 so as to be discharged from the inside.

Here, the method in which the buffer for electrophoresis is introduced into the electrode part 1200 or discharged from the inside of the electrode part 1200, or the buffer for transfer is drawn into the inside of the electrode part 1200 or discharged from the inside of the electrode part 1200 may vary. and, therefore, the scope of rights is not limited.

However, an electrophoresis buffer is automatically injected into the electrode part 1200 before electrophoresis is performed on the upper side of the receiving part 1800, and a valve (not shown) additionally provided in the electrode part 1200 when the electrophoresis is completed. ) is opened so that the buffer solution for electrophoresis is discharged from the electrode part 1200, the transfer buffer is automatically injected into the electrode part 1200 through the other side of the upper part of the electrode part 1200, and when the transfer is completed, the electrode A configuration in which a valve (not shown) additionally provided in the unit 1200 is opened will be simple.

The Western blotting apparatus 20 having the above-described configuration may further include a switch unit 1900 .

As shown in FIG. 12 , the switch unit 1900 is electrically connected to the electrode unit 1200 to control the direction of electrical stimulation applied from the electrode unit 1200 to the body unit 1400 of the receiving unit 1800 . It may be provided on the upper part.

The switch unit 1900 may include a switch body member, a sliding bar member 1920 , a contact member 1940 , and an electrode connection member 1960 .

The switch body member is coupled to the upper portion of the accommodating part, and the central side is open, and the groove part elongated in the transfer direction of the electrode part may be symmetrically formed for sliding of the sliding bar on one side and the other side with respect to the central side.

At this time, the sliding bar member 1920 is formed to be elongated in the transfer direction of the electrode part on one side and the other side of the upper surface of the switch part 1900, and may be symmetrically formed based on the open central side of the switch body member so as to slide in the groove part.

The electrode connection member 1960 is formed on the open central side of the switch body member, and two electrodes protruding upwardly on one side may be provided, and may be connected to the sliding bar member 1920 to slide.

At this time, the contact member 1940 is provided under the switch body member so that it is connected to the two electrodes of the electrode connection member 1960 and slides in the transfer direction of the electrode part 1200 in response to the movement of the sliding bar member 1920 . can be provided.

For example, when the sliding bar member 1920 is moved to the left as shown in FIG. 13 , the contact member 1940 is connected to the first electrode 1220 and electrophoresis is performed, and the sliding bar member 1920 is moved to the left. The Western blotting module 10 and the apparatus 20 according to an embodiment of the present invention are prepared to be transferred when the contact member 1940 is connected to the second electrode 1240 by moving from left to right. And there is an advantage that mass analysis is possible without being significantly affected by the experimental results by laboratory conditions, and visualization of the transferred object by the extraction member 460 or the extraction unit 1600 even when the amount of the object used for analysis is small may be possible

Next, an embodiment of a preferred cell movement observation device and cell movement observation module of the present invention in which the object of the present invention can be specifically realized will be described with reference to the accompanying drawings.

14 is a view showing a state of a cell movement observation device according to an embodiment of the present invention, and FIG. 15 is a view showing a state of a cell movement observation module of the cell movement observation device according to an embodiment of the present invention, FIG. 16 is a view showing a state in which target cells included in the second solution move in the opposite direction to gravity by the coinage factor included in the first solution in the cell migration observation module of the cell migration observation device according to an embodiment of the present invention am.

The chemotaxis described in the present invention may be a substance secreted by fibroblasts having chemotaxis to vascular endothelial cells. Substances expressing the may be included.

At this time, the first solution (A1) may be a mixture of fibroblasts secreting chemotaxis and hydrogel (H), and the second solution (A2) may be a mixture of vascular endothelial cells, which are target cells, and hydrogel (H). there is.

As shown in FIG. 14 , the cell migration observation device 30 according to an embodiment of the present invention includes a first solution A1 containing a chemotaxis for inducing cell migration, and a target moving by the chemotaxis. As a device into which the second solution A2 containing cells is injected, it may largely include a cell movement observation module 500 and a fixed supply module 600 .

First, the cell migration observation module 500 is provided so that the first solution (A1) and the second solution (A2) included in a plurality of solutions form a layer spaced apart from each other with a cell migration layer therebetween, It can play a role to check the target cells moving to the layer from the outside.

For example, the first solution (A1) is a mixture of fibroblasts secreting chemotaxis and hydrogel (H), and the second solution (A2) is a mixture of vascular endothelial cells, which are target cells, and hydrogel (H). In, the cell migration layer may be configured to include only the hydrogel (H), and thus, the target cells included in the second solution (A2) through the cell migration observation module 500 are included in the first solution (A1). The process of migrating toward the fibroblasts that secrete the chemotaxis can be confirmed through the cell migration layer.

The configuration of the cell movement observation module 500 for performing the above-described process with reference to FIG. 15 will be described in detail as follows.

The cell movement observation module 500 may largely include a body member 520 , an injection member 540 , and a connection member 560 .

First, the body member 520 may serve to accommodate a plurality of solutions including the first solution A1 and the second solution A2 provided therein with an accommodating space 522 .

Here, the front side of the receiving space 522 may be provided transparently so that the observer can check the cell movement.

In addition, the entire body member 520 is provided transparently, so that the injection process of the first solution (A1), the second solution (A2), and the hydrogel (H) forming the cell migration layer can be checked, and the first solution ( A1) and the second solution (A2) may be provided to check the formation of a layer spaced apart with a cell migration layer interposed therebetween and the movement of target cells included in the second solution (A2).

In addition, the accommodation space 522 may be provided in a rectangular shape elongated in the vertical direction to accommodate a plurality of solutions.

At this time, the body member 520 may be provided with a scale 522a for observing the movement of the object on the inner rear side of the receiving space 522 .

Specifically, the scale 522a may be a groove formed in a direction perpendicular to the longitudinal direction of the body member 520 on the inner rear side of the receiving space 522 and arranged in a plurality in the vertical direction, and at this time, to observe the movement of target cells For this, it may be prepared corresponding to the cell migration layer into which the hydrogel (H) is injected.

Furthermore, when the main body member 520 is entirely made of a transparent material, the scale 522a is provided with the scale 522a members arranged at preset intervals and is mounted and detached from the outside of the receiving space 522 to correspond to the cell migration layer. It may be arranged to be.

At this time, it may be provided so that the arrangement interval of the scale (522a) member is variable.

In addition, the main body member 520 has a cut-out groove 524 formed at the bottom, and is provided to communicate with the accommodation space 522 so that the culture solution C is introduced into the accommodation space 522 along the cut-out groove 524 from the outside. It can be arranged so that

Furthermore, a membrane (M) is provided on the upper side of the incision groove (524), that is, in a space communicating with the lower part of the receiving space (522) so that the culture solution (C) is transferred to the receiving space (522) along the membrane (M). may be

Next, the injection member 540 is injected to a position in which a plurality of solutions including the first solution (A1), the second solution (A2), and the hydrogel (H) are arranged at a predetermined interval on the body member 520 . It may be provided to be moved to the accommodation space 522 at different heights.

In this case, the injection member 540 may include an injection hole 542 and a transfer pipe 544 .

A plurality of injection holes 542 may be arranged at a predetermined interval on the upper surface of the body member 520 to inject a plurality of solutions, respectively.

Here, the injection port 542 has an upper end having a larger diameter than the lower end, and an upper end of a transfer pipe 544 to be described later is connected to the lower end, thereby facilitating the injection of a plurality of solutions, and the upper end of the inlet 542 The leak prevention member provided in the form of an inverted cone hollow inside may be fitted.

The transfer pipe 544 is bent toward one side of the receiving space 522 through the inside of the body member 520 by connecting the upper end to the lower end of the inlet 542 corresponding to the respective positions of the arranged inlet 542 and receiving It may be connected to one side of the space 522 .

In addition, the transfer pipe 544 is made of a flexible material and can be connected to the side of the receiving space 522 . In this case, the side of the receiving space 522 can adjust the height of the part to which the conveying pipe 544 is connected in the vertical direction. It may be provided so that the height of the first solution (A1) or the second solution (A2) or the cell migration layer in the vertical direction can be adjusted to a desired height.

Next, the connection member 560 may be provided on both sides of the body member 520 so that the plurality of body members 520 are connected to each other or the body member 520 is fixed to the fixed supply module 600 .

Specifically, the connection member 560 may include an unevenness 562 and a groove 564 , and the unevenness 562 may be formed to protrude from one side of the body member 520 .

The unevenness 562 may be formed to be elongated in the longitudinal direction of the body member 520, and the ends may be provided in a radial shape in the protruding direction.

The concave groove 564 may be provided to correspond to the shape of the concavo-convex 562 on the other side of the body member 520, and specifically, it may be provided to correspond to the shape of the concavo-convex 562 provided with a radial end at the protruding direction. there is.

A plurality of body members 520 may be connected to each other in a direction perpendicular to the vertical direction through the connection member 560 as described above, so that movement of a plurality of cells may be confirmed.

For example, in the device as described above, the cell movement observation module 500 is provided with a rectangular accommodating space 522 elongated in the vertical direction to accommodate the solution, and a cutout groove provided at the lower portion of the accommodating space 522 . 524 includes a body member 520 provided to communicate with the receiving space 522, and a plurality of injection ports 542 into which the solution is injected are arranged at predetermined intervals on the upper surface of the body member 520, A plurality of transfer pipes 544 connected to the lower end of the inlet 542 are bent toward the receiving space 522 in the body member 520 to correspond to the respective positions of the arranged inlet 542, and the receiving space 522 The injection member 540 connected to the side of the and the concave-convex 562 protrudingly formed on one side of the body member 520, and the concave-convex groove formed to correspond to the shape of the concave-convex 562 on the other side of the body member 520 ( 564 is provided and may include a connection member 560 connected to the object.

At this time, the injection member 540 is connected to the side of the receiving space 522 at different heights with respect to the lower surface of the receiving space 522, and the plurality of solutions are stacked in the direction of gravity. cell migration in the opposite direction of

In addition, if the body member 520 can be provided so that the inside of the receiving space 522 can be checked from the outside, the front side of the body member 520 is transparent when the entire body member 520 is made of a transparent material. If it is made of a material and is provided so that the inside of the accommodation space 522 can be checked, it will be said that all of them fall within the scope of the present invention.

At this time, the injection member 540 may also be provided so as to check the solution moving inside the transfer pipe 544 from the outside of the body member 520 .

Although not shown in the drawings, the cell movement observation module 500 having the configuration as described above is provided to enable wireless communication with the outside, photographing the movement of cells inside the receiving space 522 from the outside, and capturing the photographed image or image. It may further include a photographing member for transmitting to the outside.

Specifically, when an external user generates a photographing request through an external terminal and transmits it to the external management server, the external management server transmits a control command for photographing the interior of the accommodation space 522 to the photographing member, and the photographing member is managed externally By receiving a control command from the server, the inside of the accommodation space 522 may be photographed and transmitted to an external management server.

Finally, the external management server may be provided so that the result of cell movement can be checked remotely through the external terminal by converting the received photographed image into data and transmitting the photographed image or video to the external terminal that generated the photographing request.

On the other hand, in order to supply the culture medium (C) for cell culture into the cell movement observation module 500 as described above, the fixed supply module 600 fixes the cell movement observation module 500 therein, and the culture medium ( C) may be provided to control the amount.

Specifically, the fixed supply module 600 has a fixture 640 drawn into the groove 564 of the above-described connection member 560 on one side of the inner side is formed to protrude in the vertical direction from the lower surface, and the other side of the inner side has irregularities ( A fixing groove 620 introduced into the 562 may be formed in a vertical direction from the lower surface.

As shown in the above-mentioned FIG. 14 , the fixture 640 is arranged spaced apart from one side of the inner side of the fixed supply module 600 by a predetermined interval, and a fixing groove is formed on the other side of the inner side opposite to the inner surface of the fixed supply module 600 . The 620 is spaced apart from each other and arranged so that a plurality of connected or single body members 520 can be freely mounted or detached from the fixing member 640 or the fixing groove 620 .

The fixed supply module 600 having the configuration as described above may further include a partition member 660 .

The partition member 660 may be provided such that one side is connected to the fixing member 640 and the other side is connected to the fixing groove 620 .

That is, a concave-convex 562 formed on one side of the body member 520 is formed on one side of the partition member 660, and a recess 564 formed on the other side of the body member 520 is formed on the other side of the partition member 660 on the other side of the partition member 660. can be formed.

Therefore, there is an advantage in that it is possible to save the consumed culture solution (C) by adjusting the amount of the culture solution (C) accommodated in the fixed supply module (600) supplied to the body member (520).

By having the above-described configuration, the cell movement observation device 30 according to an embodiment of the present invention is injected through an injection tube as shown in FIG. 16 and the second solution (A2), which is sequentially stacked from the bottom, hydrogel (H), the culture solution (C) is supplied to the upper and lower portions of the receiving space 522 in which the first solution (A1) is accommodated, and the target cells containing the fluorescent material are first transformed by the chemotaxis of the first solution (A1). The process of moving from the solution (A1) in the direction of the first solution (A1), that is, in the opposite direction of gravity, can be confirmed through the cell migration layer formed between the first solution (A1) and the second solution (A2). do.

Next, a plurality of the above-described cell movement observation module 500 is arranged in a direction perpendicular to the longitudinal direction (or up and down direction) of the body member 520 without the connection member 560 , and combined cell movement observation module 2200 ) And the cell movement observation device 40 including the fixing module 2600 and the receiving module 2400 will be looked at.

Figure 17 is a view showing the appearance of a cell movement observation device according to another embodiment of the present invention, Figure 18 is a view showing the appearance of the cell movement observation module of the cell movement observation device according to another embodiment of the present invention, Figure 19 is a view for explaining a state in which the first solution, the hydrogel, and the second solution are stacked in the cell movement observation apparatus according to another embodiment of the present invention, and FIG. 20 is cell movement observation according to another embodiment of the present invention It is a view showing a state in which the cell movement observation module is introduced and fixed between the fixing members of the fixing module in the device, and the culture medium is introduced into the receiving space through the incision groove of the cell movement observation module through the culture medium supply path.

As shown in FIG. 17 , the cell movement observation device 40 according to another embodiment of the present invention may largely include a cell movement observation module 2200 , an accommodation module 2400 , and a fixing module 2600 .

First, the cell movement observation module 2200 may include a body member 2220 and an injection member 2240 .

In addition, the body member 2220 may include an accommodation space 2222 , a scale 2222a , and a cutout groove 2224 , and the injection member 2240 may include an injection hole 2242 and a transfer pipe 2244 .

The receiving space 2222, the scale 2222a, the incision groove 2224, the injection hole 2242, and the transfer pipe 2244 according to another embodiment of the present invention are in one embodiment of the present invention described with reference to Figs. Since the structure and function of the receiving space 522, the scale 522a, the cutout groove 524, the injection hole 542, and the transfer pipe 544 are identical and similar, the description will be omitted and only other parts will be described.

Here, the cell movement observation module 2200 may be provided as one unit module including only the body member 2220 and the injection member 2240 without a connection member in the cell movement observation module 500 shown in FIG. As shown in , a plurality of body members 2220 and injection members 2240 may be formed so that a plurality of unit modules are arranged in one direction.

In addition, although not shown in the drawings, like the cell movement observation module 500 described through an embodiment of the present invention, the cell movement observation module 2200 according to another embodiment of the present invention is provided to enable wireless communication with the outside, It may further include a photographing member for photographing the movement of cells inside the receiving space 2222 from the outside, and transmitting the photographed image or image to the outside.

A plurality of accommodating spaces 2222 showing how the first solution (A1), the hydrogel (H), and the second solution (A2) are stacked in the cell movement observation module 2200 according to another embodiment of the present invention through FIG. 19 . ) will be explained again.

First, the second solution A2 containing the target cells may be filled through the injection hole 2242 connected to the lower portion of the accommodation space 2222 .

Next, the hydrogel (H) is injected through the other injection port 2242 and is laminated on top of the second solution (A2) so that a cell migration layer capable of confirming the movement of target cells is stacked.

Finally, the first solution (A1) containing the fibroblasts secreting the chemotaxis is injected through another injection port 2242 to be laminated on top of the cell migration layer, and a receiving space for culturing target cells and fibroblasts (2222) The culture medium (C) is introduced into the upper and lower portions, and finally, the movement of cells in the opposite direction to the direction of gravity can be confirmed in the cell movement layer.

In order to supply such a culture solution (C), an internal space is provided and the upper part is formed so that the cell movement observation module 2200 can be fixed to the accommodation module 2400 for accommodating the culture solution (C) for cell culture. .

In this case, the accommodating module 2400 may further include a cover 2420 , and the cover 2420 may be provided to close the open surface of the accommodating module 2400 .

Here, the cell movement observation module 2200 may be fixed by the fixing module 2600 inside the receiving module 2400 .

The fixing module 2600 includes a plurality of fixing members 2620 arranged in one direction so that the perimeter specifically protrudes from the lower surface to the top, and the cell movement observation module 2200 can be introduced into the space between the perimeters. can do.

By providing a plurality of fixing members 2620 arranged in one direction as described above, a plurality of cell movement observation modules 2200 are introduced into the receiving module 2400 at once to supply a culture solution (C), or, conversely, cell movement is prevented. A plurality of completed cell movement observation modules 2200 can be withdrawn from the accommodation module 1400 at once.

In addition, the fixing module 2600 may further include a holding member 2640 .

The holding member 2640 may be provided so that the researcher can easily take out the fixed module 2600 to which the cell movement observation module 2200 is fixed in the receiving module 2400, if it can be provided so that the researcher can hold it. , shape, material, etc. may be varied, and therefore the scope of rights is not limited, of course.

However, for more detailed description, referring to the bar shown in the drawings for example, the holding member 2640 is disposed in parallel with the fixing member 2620 in the upper direction from both sides of the lower surface as shown in FIG. 20 . It is formed to protrude so that the ends are bent in opposite directions so that the ends protrude to the outside of the receiving module 2400 to be easily gripped.

In other words, the holding member 2640 is formed to protrude in the same direction as the fixing member 2620 , and an end of the holding member 2640 may be provided to be bent outwardly of the fixing module 2600 .

In this case, a culture solution supply path 2660 may be formed in the fixing module 2600 .

Specifically, the culture solution supply path 2660 serves to introduce the culture solution C into the second solution A2 containing the target cells along the incision groove 2224 of the cell movement observation module 2200, and the culture solution It will be convenient for the supply path 2660 to pass through both the fixing member 2620 and the holding member 2640 in a direction perpendicular to the longitudinal direction of the fixing member 2620 .

In addition, the culture solution supply path 2660 may be provided so that the incision groove 2224 penetrates in a fixed direction from the lower outer side to the lower inner side of the fixing module 2600, and the culture solution C is introduced into the receiving space 2222. Of course, if possible, it may be formed in a shape different from that shown in the drawings in some cases.

As described above, a preferred embodiment according to the present invention has been reviewed, and according to the cell migration observation module and the device including the same of the present invention, a first solution containing a chemotactic factor for inducing cell migration and a target cell are included. A plurality of solutions including the second solution are injected, and the first solution and the second solution included in the plurality of solutions are provided to form a layer spaced apart from the inside with a cell migration layer interposed therebetween, and move to the cell migration layer. It has the advantage of being able to observe the target cell from the outside in various ways according to the type of cell by layer, step by step.

In addition to the above-described embodiments, the fact that the present invention can be embodied in other specific forms without departing from the spirit or scope is apparent to those of ordinary skill in the art. Therefore, the above-described embodiments are to be regarded as illustrative rather than restrictive, and accordingly, the present invention is not limited to the above description, but may be modified within the scope of the appended claims and their equivalents.

Claims

a body part in which the solvent injected into the subject is accommodated and opened in one direction; and

electrode parts provided on both sides along the opening direction of the body part to extract the object to the outside of the body part;

Western blotting module containing.
According to claim 1,

The electrode part,

a first electrode member in which a current path for arranging the object in one direction is formed by separating the object in the solvent;

a second electrode member in which a current path for moving the object injected into the solvent in an open direction of the main body is formed; and

an extraction member provided adjacent to the main body to extract the object moving along the open direction of the main body;

Western blotting module comprising a.
3. The method of claim 2,

Current paths of the first electrode member and the second electrode member are characterized in that the direction is perpendicular to each other,

Western blotting module.
4. The method of claim 3,

The first electrode member,

Characterized in that the current path is formed so that the object is arranged from the top to the bottom in the solvent,

Western blotting module.
5. The method of claim 4,

The electrode part,

It is provided to surround the main body and the extraction member, characterized in that it further comprises a cover member in which the first electrode member and the second electrode member are formed in the inner space,

Western blotting module.
6. The method of claim 5,

The cover member,

a first cover 482 provided to surround one side of the main body so that the first electrode member formed on the inner surface is electrically connected to an external power source; and

a second cover that surrounds the other side of the main body and is coupled to the first cover (482) member so that the second electrode member formed on the inner surface is electrically connected to an external power source;

Western blotting module comprising a.
7. The method of claim 6,

The first electrode member,

a first electrode provided to protrude above the first cover 482 and connected to an external power source; and

a first conductive plate connected to the first electrode so that different poles are formed on the upper end and lower end of the inner surface of the first cover 482;

Western blotting module comprising a.
8. The method of claim 7,

The second electrode member,

a second electrode provided to protrude above the second cover and connected to an external power supply; and

a second conductive plate connected to the second electrode so that different poles are formed on the inner side central side of the first cover 482 and the inner side central side of the second cover;

Western blotting module comprising a.
An apparatus for separating the object or extracting the object to the outside of the solvent so that the object injected into the solvent through electrical stimulation is arranged in one direction in the solvent,

an electrode part electrically connected to an external power source so that electrical stimulation is applied to the object;

a body part that accommodates the solvent in a accommodating space provided therein, and is provided so that the object injected into the solvent to which electrical stimulation is applied can be moved to the outside in the accommodating space; and

an extraction unit provided adjacent to the main body and extracting the object moved to the outside in the accommodation space;

Western blotting apparatus comprising a.
10. The method of claim 9,

The body part,

Characterized in that the outer peripheral surface corresponding to the moving direction of the object is formed open so that the object can be moved from the receiving space to the extraction unit,

Western blotting apparatus.
11. The method of claim 10,

The body part,

Characterized in the rectangular shape formed long in the vertical direction,

Western blotting apparatus.
12. The method of claim 11,

The body part,

a first side surface provided to face each other with respect to the accommodation space, a portion of which is open; and

It characterized in that it comprises a second side connected to the first side so as to be perpendicular to the first side,

The first side is characterized in that it has a length shorter than the width of the second side,

Western blotting apparatus.
13. The method of claim 12,

The first aspect is

Characterized in that cut-out holes symmetrical to each other based on the receiving space are formed,

Western blotting apparatus.
14. The method of claim 13,

The incision hole is

characterized in that it is formed on the first side so that the object can be moved to the extraction part in a direction perpendicular to the longitudinal direction of the body part,

Western blotting apparatus.
15. The method of claim 14,

The incision hole is

Characterized in that it is a rectangle formed long in the vertical direction,

Western blotting apparatus.
15. The method of claim 14,

When the object is arranged from top to bottom inside the solvent by electrical stimulation,

The incision hole is

Characterized in that it has a length and width corresponding to the arrangement and width of the object so that the arranged object can be transferred to the extraction unit,

Western blotting apparatus.
15. The method of claim 14,

The body part,

characterized in that an adjustment member for adjusting the length and width of the transfer region is provided,

Western blotting apparatus.
18. The method according to any one of claims 12 to 17,

The body part,

Characterized in that a plurality of connections are formed in the width direction of the first side so that a plurality of objects can be transferred to the extraction unit,

Western blotting apparatus.
10. The method of claim 9,

The electrode part,

a first electrode member in which a current path for arranging the object in one direction is formed by being separated in the solvent; and

a second electrode member in which a current path for moving the object injected into the solvent from the accommodation space to the extraction unit is formed;

Western blotting apparatus comprising a.
20. The method of claim 19,

Current paths of the first electrode member and the second electrode member are characterized in that the direction is perpendicular to each other,

Western blotting apparatus.
21. The method of claim 20,

The first electrode member,

Characterized in that a current path for arranging the object in the solvent from the top to the bottom is formed,

Western blotting apparatus.
22. The method of claim 21,

The electrode part,

It is provided to surround the main body, characterized in that it further comprises a cover member in which the first electrode member and the second electrode member are formed in the inner space,

Western blotting apparatus.
23. The method of claim 22,

The cover member,

a first cover 482 provided to surround one side of the main body so that the first electrode member formed on the inner surface is electrically connected to an external power source; and

a second cover that surrounds the other side of the main body and is coupled to the first cover (482) member so that the second electrode member formed on the inner surface is electrically connected to an external power source;

Western blotting apparatus comprising a.
24. The method of claim 23,

The first electrode member,

a first electrode provided to protrude above the first cover 482 and connected to an external power source; and

a first conductive plate connected to the first electrode so that different poles are formed on the upper end and lower end of the inner surface of the first cover 482;

Western blotting apparatus comprising a.
25. The method of claim 24,

The second electrode member,

a second electrode provided to protrude above the second cover and connected to an external power supply; and

a second conductive plate connected to the second electrode so that different poles are formed on the inner side central side of the first cover 482 and the inner side central side of the second cover;

Western blotting apparatus comprising a.
21. The method of claim 20,

Further comprising a fixing part for arranging the plurality of electrode parts in the current path direction of the second electrode member,

Western blotting apparatus.
27. The method of claim 26,

The buffer solution for electrophoresis, which has one end and the other end of the solvent having different potentials, is drawn into or discharged from the inside of the electrode, so that the object is separated and arranged in one direction in the solvent by electrical stimulation. Further comprising a accommodating part accommodating the electrode part so that the transfer buffer for forming a flow of ions is drawn into the electrode part or discharged from the inside of the electrode part so that the object injected into the receiving space can be moved from the receiving space to the extraction part,

Western blotting apparatus.
28. The method of claim 27,

It is provided on the upper portion of the receiving part, and is electrically connected to the electrode part, further comprising a switch part for controlling the direction of electrical stimulation applied from the electrode part to the main body part,

Western blotting apparatus.
A cell movement observation device in which a plurality of solutions including a first solution containing a chemotaxis for inducing cell migration and a second solution containing target cells are injected,

The first solution and the second solution included in the plurality of solutions are provided to form a layer spaced apart from the inside with a cell migration layer interposed therebetween, so as to check the target cells moving to the cell migration layer from the outside cell movement observation module; and

a fixed supply module for supplying the culture solution to the cell migration observation module, provided to fix the cell movement observation module therein and to adjust the amount of the culture medium for the accommodated cell culture;

A cell movement observation device comprising a.
30. The method of claim 29,

The cell movement observation module,

a body member having a receiving space therein for accommodating the plurality of solutions;

an injection member provided such that the plurality of solutions are injected into positions arranged at a predetermined interval on the body member and moved to the receiving space at different heights; and

a connection member provided on both sides of the body member so that a plurality of body members are connected to each other or the body member is fixed to the fixed supply module;

Cell movement observation device comprising a.
31. The method of claim 30,

The connecting member is

irregularities protruding from one side of the body member; and

a groove corresponding to the shape of the unevenness on the other side of the body member;

Cell movement observation device comprising a.
31. The method of claim 30,

The receiving space is

Characterized in that it is provided in the form of a rectangular shape elongated in the vertical direction to accommodate the plurality of solutions,

Cell movement monitoring device.
33. The method of claim 32,

The body member,

characterized in that a cut-out groove communicating with the lower portion of the receiving space is provided,

Cell movement monitoring device.
31. The method of claim 30,

The injection member,

It characterized in that it comprises a plurality of injection holes arranged at predetermined intervals on the upper surface of the body member so that the plurality of solutions can be injected,

Cell movement monitoring device.
35. The method of claim 34,

The injection member,

A plurality of transfer pipes are connected to the lower end of the inlet to correspond to the respective positions of the arranged inlet ports, and are bent toward the receiving space inside the body member, characterized in that they are connected to the side surface of the receiving space,

Cell movement monitoring device.
30. The method of claim 29,

The fixed supply module,

On one side of the inner side, a fixture introduced into the groove is formed to protrude in the vertical direction from the lower surface,

Characterized in that the fixing groove leading into the unevenness on the other side of the inner surface is formed in a vertical direction from the lower surface,

Cell movement monitoring device.
37. The method of claim 36,

The fixed supply module,

Characterized in that it comprises a partition member having one side connected to the fixture and the other end connected to the fixing groove in order to control the amount of the culture solution accommodated,

Cell movement monitoring device.
The cell movement observation module of claim 29.
39. The method of claim 38,

a body member having a rectangular receiving space formed long in the vertical direction to accommodate the solution, and having a cutout groove provided at a lower portion of the receiving space to communicate with the receiving space;

A plurality of injection holes into which the solution is injected are arranged at predetermined intervals on the upper surface of the main body member, and a plurality of transfer pipes connected to the lower end of the injection holes corresponding to the respective positions of the arranged injection holes are provided inside the body member. an injection member bent toward the accommodating space and connected to a side surface of the accommodating space; and

a connection member provided with concavities and convexities protruding from one side of the body member and grooves formed to correspond to the shape of the concavities and convexities on the other side of the body member and connected to an object;

Cell movement observation module comprising a.
40. The method of claim 39,

The injection member,

Based on the lower surface of the accommodating space, a plurality of transfer pipes are connected to the side of the accommodating space at different heights,

Cell movement observation module.
40. The method of claim 39,

The body member,

characterized in that it is provided so that the inside of the accommodation space can be checked from the outside,

Cell movement observation module.
42. The method of claim 41,

The injection member,

Characterized in that it is provided so as to check the solution moving inside the transfer pipe from the outside of the body member,

Cell movement observation module.
A cell movement observation device in which a plurality of solutions including a first solution containing a chemotaxis for inducing cell migration and a second solution containing target cells are injected,

The first solution and the second solution included in the plurality of solutions are provided to form a layer spaced apart from the inside with a cell migration layer interposed therebetween, so as to check the target cells moving to the cell migration layer from the outside cell movement observation module; and

a receiving module for accommodating a culture solution for cell culture in order to supply the culture solution to the cell movement observation module; and

a fixing module for fixing the cell movement observation module inside the accommodation module;

A cell movement observation device comprising a.
44. The method of claim 43,

The cell movement observation module,

a body member having a receiving space therein for accommodating the plurality of solutions; and

an injection member provided such that the plurality of solutions are injected into positions arranged at a predetermined interval on the body member and moved to the receiving space at different heights;

Cell movement observation device comprising a.
45. The method of claim 44,

The receiving space is

Characterized in that it is provided in the form of a rectangular shape elongated in the vertical direction to accommodate the plurality of solutions,

Cell movement monitoring device.
46. The method of claim 45,

The body member,

characterized in that a cut-out groove communicating with the lower portion of the receiving space is provided,

Cell movement monitoring device.
45. The method of claim 44,

The injection member,

Characterized in that a plurality of injection holes arranged at predetermined intervals are formed on the upper surface of the body member so that the plurality of solutions can be injected,

Cell movement monitoring device.
48. The method of claim 47,

The injection member,

A plurality of transfer pipes are connected to the lower end of the inlet to correspond to the respective positions of the arranged inlet ports, and are bent toward the receiving space inside the body member, characterized in that they are connected to the side surface of the receiving space,

Cell movement monitoring device.
45. The method of claim 44,

The cell movement observation module,

A plurality of the main body member and the injection member are formed, characterized in that they are arranged in one direction,

Cell movement monitoring device.
44. The method of claim 43,

The fixed module is

Characterized in that it includes a plurality of fixing members that protrude upward from the lower surface and are arranged in one direction so that the cell movement observation module can be introduced into the space between them,

Cell movement monitoring device.
51. The method of claim 50,

The fixed module is

Characterized in that the holding member is formed to protrude upward from both sides of the lower surface so as to be arranged in parallel with the fixing member so that the ends are bent in opposite directions,

Cell movement monitoring device.
52. The method of claim 51,

The fixed module is

A culture solution supply path for introducing the culture solution into the second solution along the incision groove of the cell movement observation module is formed through the fixing member and the holding member in a direction perpendicular to the longitudinal direction of the fixing member. ,

Cell movement monitoring device.