WO2022035382A1 - Système et procédé de prétraitement automatisé d'échantillons - Google Patents

Système et procédé de prétraitement automatisé d'échantillons Download PDF

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Publication number
WO2022035382A1
WO2022035382A1 PCT/SG2021/050476 SG2021050476W WO2022035382A1 WO 2022035382 A1 WO2022035382 A1 WO 2022035382A1 SG 2021050476 W SG2021050476 W SG 2021050476W WO 2022035382 A1 WO2022035382 A1 WO 2022035382A1
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WO
WIPO (PCT)
Prior art keywords
sample
tube
sample tube
tubes
capped
Prior art date
Application number
PCT/SG2021/050476
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English (en)
Inventor
Min-Han Tan
Geok Soon LIM
Original Assignee
Lucence Life Sciences Pte. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lucence Life Sciences Pte. Ltd. filed Critical Lucence Life Sciences Pte. Ltd.
Publication of WO2022035382A1 publication Critical patent/WO2022035382A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00524—Mixing by agitating sample carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00742—Type of codes
    • G01N2035/00752—Type of codes bar codes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00742—Type of codes
    • G01N2035/00772—Type of codes mechanical or optical code other than bar code
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G01N2035/00821—Identification of carriers, materials or components in automatic analysers nature of coded information
    • G01N2035/00831—Identification of carriers, materials or components in automatic analysers nature of coded information identification of the sample, e.g. patient identity, place of sampling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0403—Sample carriers with closing or sealing means
    • G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0418—Plate elements with several rows of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/046—General conveyor features
    • G01N2035/0465—Loading or unloading the conveyor

Definitions

  • the present disclosure relates broadly, but not exclusively, to automated systems and methods for pre-processing biological samples such as saliva/ sputum.
  • Saliva may be a preferred sample for mass testing of respiratory diseases such as COVID-19, MERS, etc. as compared to invasive swabs because it is accurate, easier to collect, and the risks of infection through aerosol are reduced for healthcare workers during specimen collection.
  • saliva and sputum solubilization is manual, gives rise to heterogeneous samples if not properly mixed, and can even be physically painful or injurious for medical laboratory staff because of repeated stress injury from sample shaking/mixing requirements. Diverse laboratory practices to solubilize saliva samples, or even worse, the omission of this step, can lead to reduced test accuracy.
  • sample pooling procedures allow for mass screening and surveillance testing to monitor for a community- or population-level occurrence, such as an infectious disease outbreak.
  • Sample pooling has several advantages including improved sample throughput and reduced cost per test.
  • sample pooling of during testing for respiratory infections are typically performed on nasopharyngeal or oropharyngeal swabs, nasal swabs, or midturbinate swabs but not on saliva / sputum samples due to the effects of sample dilution which can result in reduced test sensitivities.
  • An aspect of the present disclosure provides an automated sample preprocessing system, comprising: a first liquid handling module configured to:
  • Another aspect of the present disclosure provides an automated sample preprocessing method, comprising:
  • Figure 1 A shows a perspective view of an automated sample pre-processing system according to an example embodiment.
  • Figure 1 B shows a plan view of the system of Figure 1 A.
  • Figure 2 shows a simplified diagram of the system of Figure 1 A during sample tray loading.
  • Figure 3 shows a partial view of the system of Figure 1 A during bar code scanning.
  • Figure 4 shows a partial view of the system of Figure 1 A during uncapping of a sample tube.
  • Figure 5 shows a partial view of the system of Figure 1 A during dispensing of a reagent into the sample tube.
  • Figure 6 shows a partial view of the system of Figure 1A during capping of the sample tube.
  • Figure 7 shows a partial view of the system of Figure 1 A during mixing.
  • Figure 8 shows a partial view of the system of Figure 1A during transferring from mixer to output tray.
  • Figure 9 shows a plan view of an automated sample pre-processing system according to an alternate embodiment.
  • Figure 10 shows a perspective view of an implementation to fully enclose an automated sample pre-processing system according to an example embodiment.
  • Figure 1 1 shows a flow chart illustrating an automated sample pre-processing method according to an example embodiment.
  • the present disclosure provides an automated saliva / sputum solubilization, pooling and pre-concentration workflow and system that may allow a robust, standardized, and repeatable process to pre-process heterogenous and nonNewtonian saliva / sputum samples into a more homogenous and less viscous sample for enhanced downstream molecular testing.
  • the automated workflow comprises some or all of the following:
  • a solubilization reagent e.g. dithiothreitol (DTT), or proteinase K
  • DTT dithiothreitol
  • proteinase K proteinase K
  • Figure 1 A shows a perspective view of an automated sample pre-processing system 100 according to an example embodiment.
  • Figure 1 B shows a plan view of the system 100 of Figure 1 A.
  • the system 100 includes a plurality of sensors, actuators, timers, etc. and a control circuit arranged to activate components of the system 100 to perform relevant actions.
  • sensors directly used in the workflow other sensors to detect whether a disposal bin needs to be emptied, whether consumables need to be topped up, etc. may be incorporated, together with displays providing outputs (e.g. error messages) from such sensors.
  • outputs e.g. error messages
  • the system 100 includes a first robotic liquid handling module 102, a mixing module 104 and an incubation module 108.
  • the liquid handling module 102 is configured to uncap a sample tube 104, which initially contains a biological sample, dispense a predefined volume of a solubilization reagent into the uncapped sample tube 104, and cap the uncapped sample tube 104, which then contains the biological sample and the solubilization reagent.
  • the mixing module 106 is configured to mix the biological sample and the solubilization reagent in the capped sample tube 104 received from the liquid handling module 102 to obtain a mixed sample
  • the incubation module 108 is configured to incubate the mixed sample received from the mixing module based on a predefined parameter to obtain a pre- processed sample. Further actions can be performed with respect to the pre- processed sample depending on practical requirements.
  • the primary saliva/sputum sample tubes 104 are labelled with respective optically-readable identifiers (e.g. barcodes or QR codes) and placed in a first tube holder tray (herein termed as input tray 202).
  • the input tray 202 comprises an array/grid and typically organizes the primary sample tubes 104 in a 24-tube (i.e. 4x6), 48-tube (i.e. 6x8), or 96-tube (i.e. 8x12) format. Other formats are possible, as will be appreciated by a person skilled in the art.
  • the input tray 202 may subsequently be loaded or inserted by an operator into a designated loading position of the system 100 for sample preprocessing.
  • the input tray 202 is transported to the next station or module of the system 100, for example, by means of a motorized axis, conveyor belt system or robotic gripper.
  • the barcode scanner 304 subsequently reads the 1 D/2D barcodes on the labelled sample tubes 104, and stores the sample IDs in a database or readable sample list file (e.g. .txt, .csv, .dat, .xml).
  • a database or readable sample list file e.g. .txt, .csv, .dat, .xml.
  • the sample tubes 104 are uncapped sequentially in a single-channel format (i.e. one at a time) using a uncapping/capping mechanism 402.
  • the uncapping/capping mechanism 402 has swappable adaptors 404 to permit compatibility with a wide range of sample tubes from different manufacturers.
  • the adaptors 404 can work with sample tubes of different heights and/or diameters, thereby improving versatility of the system 100.
  • the single-channel uncapping format also can minimize sample cross-contamination via aerosol generation and release during the uncapping process.
  • An example suitable uncapping/capping mechanism is the rotary gripper module EHMD from Festo Group.
  • a fixed volume of a solubilization reagent e.g. DTT or proteinase K
  • a solubilization reagent helps to reduce the viscosity of the saliva / sputum samples, in order to enhance downstream molecular testing.
  • 250pl_ of 500 mM DTT solution (Promega, Ref V3151 ) is dispensed into each sample tube 104 containing 4ml_ of saliva sample and the SAFERTM Sample Stabilization Fluid to aid in the solubilization of the viscous samples.
  • sample tubes 104 are recapped sequentially in a single-channel format (i.e. one at a time) as shown in Figure 6, using the same uncapping/capping mechanism 402 as described above with reference to Figure 4.
  • the uncapping/capping mechanism 402 and dispenser 502 can be collectively considered a liquid handling module in which successive actions are coordinated. For example, as shown in Figures 4-6, after uncapping the sample tube 104, the uncapping/capping mechanism 402 moves upward, allowing a swing arm 504 of the dispenser 502 to move into position to dispense the reagent. Once the dispensing is completed, the swing arm 504 swivels back and the uncapping/capping mechanism 402 moves downward to re-cap the sample tube 104.
  • the sample tubes 104 are mixed to ensure homogenization of the sample with the solubilization reagent.
  • a robotic gripper transfers the sample tubes 104 onto a vortex mixer 702 which oscillates at a sufficiently high speed (rpm) for a period of time to result in sample fluids circulating and undergoing turbulent flow for effective mixing.
  • the vortex mixer may operate at a speed between 500 and 3000 rpm for a duration between 1 to 10 seconds, or longer, based on practical requirements.
  • the sample tubes 104 are loaded by the first robotic tube transfer module, e.g. using similar mechanism as the robotic gripper 302 in Figure 3, back to the input tray 202 at the respective positions where they are initially placed. This can both reduce the number of items required to run the system and ensure that each sample tube 104 is consistently associated with a position on the input tray 202.
  • the sample tubes 104 may be loaded onto a second tube holder tray (herein termed as output tray 802), in a complementary orientation or layout to that of the input tray 202. Such arrangement can similarly ensure that the sample ID sequence list is maintained such that the sample layout in the input tray 202 is complementary to that in the output tray 802.
  • Figure 8 shows one sample tube 104 stored on the output tray 802.
  • the output tray 802 once the output tray 802 has been filled with sample tubes, it is transported to a designated unloading position by means of a motorized axis, conveyor belt system or robotic gripper before further steps are taken. In alternate embodiments, the further steps may take place in a continuous sequence without the output tray 802 being unloaded.
  • an operator or a robot may remove the output tray 802 and incubate the sample tubes 104 at a particular temperature and for a period of time for completion of the solubilization process.
  • the sample tubes 104 are incubated at room temperature for at least 15 minutes. If a higher or lower incubation temperature is desired, the output tray may be placed in an incubator. In alternate embodiments, the sample tubes 104 may be incubated without the output tray 802 being removed. Following incubation, the pre-processed samples may be used directly for downstream molecular testing or may undergo additional treatments as described below.
  • multiple pre- processed samples in the primary sample tubes 104 are pooled together into a single tube.
  • a robotic handler may transfer either a portion or an entirety of the pre-processed sample volume from each sample tube 104 in a sequential order into a common single tube (herein termed as the pre-concentration tube).
  • Example ratios for pooling maybe 2:1 , 3:1 , 4:1 , 5:1 , 6:1 , 7:1 , 8:1 , 9:1 , 10:1 , etc. depending on practical requirements.
  • An optional general filter e.g. micron-scale filter membranes
  • medium speed centrifugation step may be included to remove any particulates or residues that may otherwise adversely clog or hinder downstream processes.
  • a robotic tube transfer system may transfer the pre-concentration tube onto a vacuum / suction pump module, or a centrifuge module to perform nucleic acid virus pre-concentration based on membranes or filters.
  • This step may significantly reduce the initial pooled sample volume to a working volume range of, for example, 100pl_ to 500pl_ depending on the viscosity of the pooled sample.
  • This additional step may also increase viral load in the pooled sample to increase test sensitivities.
  • This concentrated pooled sample may subsequently be transferred into a clean / sterile tube for downstream processing (e.g. ribonucleic acid (RNA) extraction, polymerase chain reaction (PCR) plate setup).
  • the transfer step may be specially calibrated to avoid transfer of any particulates or residues resulting from the previous upstream pre-concentration process.
  • Figure 9 shows a plan view of an automated sample pre-processing system 900 according to an alternate embodiment.
  • System 900 is similar to system 100 in several aspects.
  • system 900 also includes a turntable 901 for performing sequential steps.
  • system 900 also includes a labelling module 902 and a second robotic liquid handling module 904.
  • a plurality of primary sample tubes 906, each containing a respective sample, as well as a plurality of empty and unlabelled secondary tubes 908 are loaded onto the system 900.
  • the primary sample tubes 906 are placed in a primary tray 907, and the secondary tubes 908 are placed in a secondary tray 909.
  • the optically-readable identifiers e.g. barcodes or QR codes
  • a robotic arm picks up an empty secondary tube 908 from the secondary tray 909, and the labelling module 902 labels the empty secondary tube 908 based on at least one identifier, such that the labelled empty tube corresponds to at least one sample tube.
  • one secondary tube may correspond to one primary tube when a direct sample transfer is desired, while one secondary tube may correspond to multiple primary tubes when sample pooling is desired.
  • the labelling module 902 may employ a thermal printer to perform the concurrent labelling.
  • primary tray 907 and secondary tray 909 are affixed with optically-readable identifiers (e.g. barcodes or QR codes) that are scanned when the trays are placed in the system 900.
  • optically-readable identifiers e.g. barcodes or QR codes
  • the at least one primary sample tube 906 undergoes uncapping, dispensing, capping, mixing and incubation in the same manner as described above with reference to Figures 4-8.
  • the uncapping, dispensing and capping may be performed at station 4 of the turntable 901 , while the mixing is performed at station 5 of the turntable 901.
  • the at least one primary sample tube 906 is returned to the primary tray 907 at the position where it was originally taken from, while the labelled secondary tube 908 is stored in the secondary tray 909.
  • the primary sample tube 906 containing the pre-processed sample is transferred from the primary tray 907 to the second robotic liquid handling module 904, for example, by means of a robotic tube transfer module (e.g. a robotic arm).
  • a robotic tube transfer module e.g. a robotic arm
  • a primary sample tube which is ready for transfer or further treatments can be transferred from the primary tray 907 without having to wait for all the samples to be incubated. Such arrangement may improve the throughput of the system.
  • the primary sample tube 906 may be picked from the primary tray 907 and placed at station 1 of the turntable 901.
  • the primary sample tube 906 then undergoes mixing, e.g. using a vortex mixer, to ensure that sample molecules are evenly distributed.
  • the identifier of the sample tube 906 is read at station 2 of the turntable 901 and the sample tube 906 is moved on to the liquid handling module 904 at station 4 of the turntable 901 .
  • the primary sample tube 906 is uncapped, a predefined volume of the pre-processed sample is withdrawn from the uncapped primary sample tube 906, and the primary sample tube 906 is then recapped.
  • the withdrawn pre-processed sample is transferred to the corresponding labelled secondary tube 908 on the secondary tray 909.
  • a pipette arm may be used to withdraw the sample from the primary sample tube 906 and transfer the withdrawn sample to the labelled secondary tube 908.
  • the used pipette tip is automatically discarded and replaced by a fresh pipette tip to avoid crosscontamination.
  • the steps performed by the liquid handling module 904 may be repeated with other primary sample tubes in the output tray.
  • the example embodiment as shown in Figure 9 integrate additional modules to further improve the workflow, by ensuring that the empty secondary tubes are labelled correctly based on desired corresponding primary sample tubes and by minimising idle time.
  • the second liquid handling module 904 can help to perform sample pooling or sample transfer for downstream molecular testing, as described above, in a continuous workflow.
  • FIG 10 shows a perspective view of an implementation to fully enclose a sample pre-processing system, such as system 100 of Figure 1A or system 900 of Figure 9 according to an example embodiment.
  • the automated workflow may be performed in a fully or partially-enclosed environment with accompanying HEPA filters and/or laminar airflow systems.
  • HEPA filters 1002, 1004 may be disposed at air inlets while HEPA filter 1006 is disposed at air outlet.
  • a dedicated exhaust system exterior to the automated instrument, in the form of fan unit 1008, can keep the interior under negative pressure. Additional airflow sensors and alarms may allow the operator to monitor the internal negative pressure at all times. Further, an ultraviolet (UV) lamp allows the interior to be sterilized during regular maintenance.
  • UV ultraviolet
  • the sample pre-processing system may be disposed in a biosafety cabinet hood, such as one manufactured by ESCO Lifesciences Group, which can function with the cabinet door open.
  • a biosafety cabinet hood such as one manufactured by ESCO Lifesciences Group
  • Such cabinet may include open fronts for operator access, and utilise vertical laminar airflow where external air is run through a filter before it gets inside the cabinet and internal air is HEPA-filtered before it is released outside.
  • Figure 11 shows a flow chart 1100 illustrating an automated sample preprocessing method according to an example embodiment.
  • a sample tube containing a biological sample is uncapped.
  • a predefined volume of a solubilization reagent is dispensed into the uncapped sample tube.
  • the uncapped sample tube containing the biological sample and the solubilization reagent is capped.
  • the biological sample and the solubilization reagent in the capped sample tube are mixed to obtain a mixed sample.
  • the mixed sample is incubated based on a predefined parameter to obtain a pre- processed sample.
  • the system and method according to the present disclosure can reduce operators’ hands-on-time, thereby reducing manual labour and repeated stress injuries, reduce sample pre-processing time, allowing for ease of scaling up for mass testing and screening of large populations. Further, the system and method according to the present disclosure can improve sample testing throughput, making it more amenable to mass population-wide screening applications, and increase nucleic acid virus concentrations which would otherwise be diluted during the sample pooling process.
  • Table 1 shows an example comparing the time spent between a manual approach and the automated processing according to the present disclosure. It can be seen from Table 1 that there is a significant reduction of operator hands-on time through use of the system and method as disclosed, improving throughput of 1 Medical Lab Technologist (MLT) by 582%, and increasing testing capacity to 3000 samples/day/machine.
  • MLT Medical Lab Technologist

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Un système de prétraitement automatisé d'échantillon comprend un premier module de manipulation de liquide et un premier module de mélange. Le premier module de manipulation de liquide est conçu pour déboucher un tube d'échantillon contenant un échantillon biologique, distribuer un volume prédéfini d'un réactif de solubilisation dans le tube d'échantillon débouché, et boucher le tube d'échantillon débouché, le tube d'échantillon débouché contenant l'échantillon biologique et le réactif de solubilisation. Le premier module de mélange est conçu pour mélanger l'échantillon biologique et le réactif de solubilisation dans le tube d'échantillon bouché reçu en provenance du premier module de manipulation de liquide afin d'obtenir un premier échantillon mélangé.
PCT/SG2021/050476 2020-08-14 2021-08-16 Système et procédé de prétraitement automatisé d'échantillons WO2022035382A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10202007826S 2020-08-14
SG10202007826S 2020-08-14

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02275362A (ja) * 1989-04-17 1990-11-09 Hitachi Ltd 核酸試料調整装置
EP2458387A2 (fr) * 2010-11-30 2012-05-30 Kabushiki Kaisha Yaskawa Denki Système de traitement de spécimens
US20120318076A1 (en) * 2011-06-20 2012-12-20 Dietmar Kappelhoff System for processing closed sample tubes
US20120321516A1 (en) * 2011-06-20 2012-12-20 Gottlieb Schacher Device for decapping and recapping sample tubes
US20140241946A1 (en) * 2007-04-06 2014-08-28 Qiagen Gaithersburg, Inc. Open platform automated sample processing system
US20160114322A1 (en) * 2013-04-19 2016-04-28 Qichao Pan Parallelized sample handling
US20190302135A1 (en) * 2018-03-29 2019-10-03 Sysmex Corporation Sample pretreatment apparatus, robotic arm, and sample pretreatment method
WO2020132279A1 (fr) * 2018-12-19 2020-06-25 Nuclein, Llc Appareil et méthodes de diagnostic moléculaire

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02275362A (ja) * 1989-04-17 1990-11-09 Hitachi Ltd 核酸試料調整装置
US20140241946A1 (en) * 2007-04-06 2014-08-28 Qiagen Gaithersburg, Inc. Open platform automated sample processing system
EP2458387A2 (fr) * 2010-11-30 2012-05-30 Kabushiki Kaisha Yaskawa Denki Système de traitement de spécimens
US20120318076A1 (en) * 2011-06-20 2012-12-20 Dietmar Kappelhoff System for processing closed sample tubes
US20120321516A1 (en) * 2011-06-20 2012-12-20 Gottlieb Schacher Device for decapping and recapping sample tubes
US20160114322A1 (en) * 2013-04-19 2016-04-28 Qichao Pan Parallelized sample handling
US20190302135A1 (en) * 2018-03-29 2019-10-03 Sysmex Corporation Sample pretreatment apparatus, robotic arm, and sample pretreatment method
WO2020132279A1 (fr) * 2018-12-19 2020-06-25 Nuclein, Llc Appareil et méthodes de diagnostic moléculaire

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